LOCUS       DAYYHS010000001        86066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000001 DAYYHS010000000
VERSION     DAYYHS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.001"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..1195
                     /locus_tag="ACHA6A_000001"
     CDS             71..1195
                     /locus_tag="ACHA6A_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8968245.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1220..1399
                     /locus_tag="ACHA6A_000002"
     CDS             1220..1399
                     /locus_tag="ACHA6A_000002"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968246.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            1422..2339
                     /locus_tag="ACHA6A_000003"
     CDS             1422..2339
                     /locus_tag="ACHA6A_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8968247.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2332..3165
                     /locus_tag="ACHA6A_000004"
     CDS             2332..3165
                     /locus_tag="ACHA6A_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8968248.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3248..4366
                     /locus_tag="ACHA6A_000005"
     CDS             3248..4366
                     /locus_tag="ACHA6A_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8968249.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4387..6258
                     /locus_tag="ACHA6A_000006"
     CDS             4387..6258
                     /locus_tag="ACHA6A_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8968250.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            6252..7475
                     /locus_tag="ACHA6A_000007"
     CDS             6252..7475
                     /locus_tag="ACHA6A_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8968251.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7561..9495)
                     /locus_tag="ACHA6A_000008"
     CDS             complement(7561..9495)
                     /locus_tag="ACHA6A_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8968252.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            9721..10377
                     /locus_tag="ACHA6A_000009"
     CDS             9721..10377
                     /locus_tag="ACHA6A_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8968253.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(10451..10804)
                     /locus_tag="ACHA6A_000010"
     CDS             complement(10451..10804)
                     /locus_tag="ACHA6A_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8968254.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            10996..11925
                     /locus_tag="ACHA6A_000011"
     CDS             10996..11925
                     /locus_tag="ACHA6A_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8968255.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11936..12772
                     /locus_tag="ACHA6A_000012"
     CDS             11936..12772
                     /locus_tag="ACHA6A_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8968256.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            13020..13796
                     /locus_tag="ACHA6A_000013"
     CDS             13020..13796
                     /locus_tag="ACHA6A_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8968257.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            13972..14715
                     /locus_tag="ACHA6A_000014"
     CDS             13972..14715
                     /locus_tag="ACHA6A_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968258.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            14708..16288
                     /locus_tag="ACHA6A_000015"
     CDS             14708..16288
                     /locus_tag="ACHA6A_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8968259.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKKPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(16539..17018)
                     /locus_tag="ACHA6A_000016"
     CDS             complement(16539..17018)
                     /locus_tag="ACHA6A_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8968260.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(17034..17786)
                     /locus_tag="ACHA6A_000017"
     CDS             complement(17034..17786)
                     /locus_tag="ACHA6A_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8968261.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17903..17973
                     /locus_tag="ACHA6A_000018"
     tRNA            17903..17973
                     /locus_tag="ACHA6A_000018"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17935..17937,aa:Gly,seq:ccc)
     gene            18334..19059
                     /locus_tag="ACHA6A_000019"
     CDS             18334..19059
                     /locus_tag="ACHA6A_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8968262.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            19314..19580
                     /locus_tag="ACHA6A_000020"
     CDS             19314..19580
                     /locus_tag="ACHA6A_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8968263.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            19577..20008
                     /gene="ruvX"
                     /locus_tag="ACHA6A_000021"
     CDS             19577..20008
                     /gene="ruvX"
                     /locus_tag="ACHA6A_000021"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8968264.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            20033..20338
                     /locus_tag="ACHA6A_000022"
     CDS             20033..20338
                     /locus_tag="ACHA6A_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8968265.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20419..21564)
                     /locus_tag="ACHA6A_000023"
     CDS             complement(20419..21564)
                     /locus_tag="ACHA6A_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8968266.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21625..22272)
                     /locus_tag="ACHA6A_000024"
     CDS             complement(21625..22272)
                     /locus_tag="ACHA6A_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8968267.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            22466..22759
                     /locus_tag="ACHA6A_000025"
     CDS             22466..22759
                     /locus_tag="ACHA6A_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968268.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            22803..23114
                     /locus_tag="ACHA6A_000026"
     CDS             22803..23114
                     /locus_tag="ACHA6A_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968269.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            23111..23735
                     /locus_tag="ACHA6A_000027"
                     /pseudo
     CDS             23111..23735
                     /locus_tag="ACHA6A_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            23876..24034
                     /locus_tag="ACHA6A_000028"
     CDS             23876..24034
                     /locus_tag="ACHA6A_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968270.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            24035..24397
                     /locus_tag="ACHA6A_000029"
     CDS             24035..24397
                     /locus_tag="ACHA6A_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968271.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            24434..25282
                     /locus_tag="ACHA6A_000030"
     CDS             24434..25282
                     /locus_tag="ACHA6A_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8968272.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            25272..26747
                     /locus_tag="ACHA6A_000031"
     CDS             25272..26747
                     /locus_tag="ACHA6A_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8968273.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            27013..27420
                     /locus_tag="ACHA6A_000032"
     CDS             27013..27420
                     /locus_tag="ACHA6A_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8968274.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            27423..27638
                     /locus_tag="ACHA6A_000033"
     CDS             27423..27638
                     /locus_tag="ACHA6A_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968275.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            27642..28022
                     /locus_tag="ACHA6A_000034"
     CDS             27642..28022
                     /locus_tag="ACHA6A_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8968276.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            28156..28311
                     /locus_tag="ACHA6A_000035"
     CDS             28156..28311
                     /locus_tag="ACHA6A_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8968277.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            28380..28853
                     /locus_tag="ACHA6A_000036"
     CDS             28380..28853
                     /locus_tag="ACHA6A_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8968278.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            28850..30544
                     /locus_tag="ACHA6A_000037"
     CDS             28850..30544
                     /locus_tag="ACHA6A_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8968279.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            30510..30695
                     /locus_tag="ACHA6A_000038"
     CDS             30510..30695
                     /locus_tag="ACHA6A_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968280.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            30699..31874
                     /locus_tag="ACHA6A_000039"
     CDS             30699..31874
                     /locus_tag="ACHA6A_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8968281.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            31867..33390
                     /locus_tag="ACHA6A_000040"
     CDS             31867..33390
                     /locus_tag="ACHA6A_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8968282.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            33446..33730
                     /locus_tag="ACHA6A_000041"
     CDS             33446..33730
                     /locus_tag="ACHA6A_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8968283.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            33717..34052
                     /locus_tag="ACHA6A_000042"
     CDS             33717..34052
                     /locus_tag="ACHA6A_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8968284.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            34347..34973
                     /locus_tag="ACHA6A_000043"
     CDS             34347..34973
                     /locus_tag="ACHA6A_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968285.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            35715..36551
                     /locus_tag="ACHA6A_000044"
     CDS             35715..36551
                     /locus_tag="ACHA6A_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8968286.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            36615..37046
                     /locus_tag="ACHA6A_000045"
     CDS             36615..37046
                     /locus_tag="ACHA6A_000045"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8968287.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(37108..38430)
                     /locus_tag="ACHA6A_000046"
     CDS             complement(37108..38430)
                     /locus_tag="ACHA6A_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8968288.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(38584..40224)
                     /locus_tag="ACHA6A_000047"
     CDS             complement(38584..40224)
                     /locus_tag="ACHA6A_000047"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8968289.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            40557..41027
                     /locus_tag="ACHA6A_000048"
     CDS             40557..41027
                     /locus_tag="ACHA6A_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8968290.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            41249..42352
                     /locus_tag="ACHA6A_000049"
     CDS             41249..42352
                     /locus_tag="ACHA6A_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8968291.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            42636..43316
                     /locus_tag="ACHA6A_000050"
     CDS             42636..43316
                     /locus_tag="ACHA6A_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8968292.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            43406..44440
                     /locus_tag="ACHA6A_000051"
     CDS             43406..44440
                     /locus_tag="ACHA6A_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8968293.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            44454..44789
                     /locus_tag="ACHA6A_000052"
     CDS             44454..44789
                     /locus_tag="ACHA6A_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968294.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            44773..47253
                     /locus_tag="ACHA6A_000053"
     CDS             44773..47253
                     /locus_tag="ACHA6A_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968295.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            47309..48868
                     /locus_tag="ACHA6A_000054"
     CDS             47309..48868
                     /locus_tag="ACHA6A_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8968296.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            48865..50229
                     /locus_tag="ACHA6A_000055"
     CDS             48865..50229
                     /locus_tag="ACHA6A_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8968297.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            50400..51470
                     /locus_tag="ACHA6A_000056"
     CDS             50400..51470
                     /locus_tag="ACHA6A_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8968298.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            51485..52153
                     /locus_tag="ACHA6A_000057"
     CDS             51485..52153
                     /locus_tag="ACHA6A_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968299.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            52173..52751
                     /locus_tag="ACHA6A_000058"
     CDS             52173..52751
                     /locus_tag="ACHA6A_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8968300.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            53152..54063
                     /locus_tag="ACHA6A_000059"
     CDS             53152..54063
                     /locus_tag="ACHA6A_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8968301.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(54441..55358)
                     /gene="rnhC"
                     /locus_tag="ACHA6A_000060"
     CDS             complement(54441..55358)
                     /gene="rnhC"
                     /locus_tag="ACHA6A_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8968302.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            55560..55997
                     /gene="zapA"
                     /locus_tag="ACHA6A_000061"
     CDS             55560..55997
                     /gene="zapA"
                     /locus_tag="ACHA6A_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8968303.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            56055..56603
                     /locus_tag="ACHA6A_000062"
     CDS             56055..56603
                     /locus_tag="ACHA6A_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM8968304.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            56718..59078
                     /locus_tag="ACHA6A_000063"
     CDS             56718..59078
                     /locus_tag="ACHA6A_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8968305.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            59165..59479
                     /gene="trxA"
                     /locus_tag="ACHA6A_000064"
     CDS             59165..59479
                     /gene="trxA"
                     /locus_tag="ACHA6A_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8968306.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            59661..61439
                     /gene="uvrC"
                     /locus_tag="ACHA6A_000065"
     CDS             59661..61439
                     /gene="uvrC"
                     /locus_tag="ACHA6A_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8968307.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            61584..62483
                     /locus_tag="ACHA6A_000066"
     CDS             61584..62483
                     /locus_tag="ACHA6A_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968308.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            62476..63738
                     /locus_tag="ACHA6A_000067"
     CDS             62476..63738
                     /locus_tag="ACHA6A_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8968309.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            63899..65797
                     /locus_tag="ACHA6A_000068"
     CDS             63899..65797
                     /locus_tag="ACHA6A_000068"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8968310.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(66127..66846)
                     /locus_tag="ACHA6A_000069"
     CDS             complement(66127..66846)
                     /locus_tag="ACHA6A_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8968311.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    67036..67083
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            67183..67515
                     /locus_tag="ACHA6A_000070"
     CDS             67183..67515
                     /locus_tag="ACHA6A_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968312.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            67502..69505
                     /locus_tag="ACHA6A_000071"
     CDS             67502..69505
                     /locus_tag="ACHA6A_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8968313.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            69524..69994
                     /locus_tag="ACHA6A_000072"
     CDS             69524..69994
                     /locus_tag="ACHA6A_000072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8968314.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            70015..70599
                     /locus_tag="ACHA6A_000073"
     CDS             70015..70599
                     /locus_tag="ACHA6A_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM8968315.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            70614..71600
                     /locus_tag="ACHA6A_000074"
     CDS             70614..71600
                     /locus_tag="ACHA6A_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8968316.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            71590..71901
                     /locus_tag="ACHA6A_000075"
     CDS             71590..71901
                     /locus_tag="ACHA6A_000075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8968317.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            71923..73704
                     /locus_tag="ACHA6A_000076"
     CDS             71923..73704
                     /locus_tag="ACHA6A_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8968318.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            73697..75073
                     /locus_tag="ACHA6A_000077"
     CDS             73697..75073
                     /locus_tag="ACHA6A_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8968319.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            75080..75715
                     /locus_tag="ACHA6A_000078"
     CDS             75080..75715
                     /locus_tag="ACHA6A_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8968320.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            75745..77100
                     /locus_tag="ACHA6A_000079"
     CDS             75745..77100
                     /locus_tag="ACHA6A_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8968321.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(77292..78686)
                     /locus_tag="ACHA6A_000080"
     CDS             complement(77292..78686)
                     /locus_tag="ACHA6A_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8968322.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            78778..79749
                     /locus_tag="ACHA6A_000081"
     CDS             78778..79749
                     /locus_tag="ACHA6A_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8968323.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            79746..80528
                     /locus_tag="ACHA6A_000082"
     CDS             79746..80528
                     /locus_tag="ACHA6A_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8968324.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(80697..81068)
                     /locus_tag="ACHA6A_000083"
     CDS             complement(80697..81068)
                     /locus_tag="ACHA6A_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8968325.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            81202..81645
                     /locus_tag="ACHA6A_000084"
     CDS             81202..81645
                     /locus_tag="ACHA6A_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8968326.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(81688..82497)
                     /gene="yidA"
                     /locus_tag="ACHA6A_000085"
     CDS             complement(81688..82497)
                     /gene="yidA"
                     /locus_tag="ACHA6A_000085"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8968327.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(82516..83883)
                     /locus_tag="ACHA6A_000086"
     CDS             complement(82516..83883)
                     /locus_tag="ACHA6A_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8968328.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(83867..84697)
                     /locus_tag="ACHA6A_000087"
     CDS             complement(83867..84697)
                     /locus_tag="ACHA6A_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8968329.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            84860..85297
                     /locus_tag="ACHA6A_000088"
     CDS             84860..85297
                     /locus_tag="ACHA6A_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8968330.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            85373..85993
                     /gene="rpoE"
                     /locus_tag="ACHA6A_000089"
     CDS             85373..85993
                     /gene="rpoE"
                     /locus_tag="ACHA6A_000089"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8968331.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        28362 a        14556 c        17845 g        25303 t
ORIGIN      
        1 tactaattcc gttaatatac ataagtgaaa gcgttttata taagaacttg aaaagaaaga
       61 agggatagac atgaaaaaaa gatacaaatt ggtgatagct ttgctaaaca ttaccctatt
      121 attcagctta agtgcatgtg gaaaaactaa cgaagaagta acagcaaaca tcgcagatcc
      181 aatgataaaa aaagccacta cattagaagt atatgtcgca aacggcaaac tgacaaacta
      241 tgttgaaaat gctgcaaaaa tctataaccg caaaactgga gcaaatattg acttaaaaat
      301 tgtgaatgta gcttcgggaa cagcaacagt acaaatgatc accccaaaat tggtaacaca
      361 cgaagatatg ccggatatta tctccatggc agacacttca gcagcaggtg tgctggaaaa
      421 atttcaagat gctttttata gtgctgatga atatggattt tatgacaaat atggcgatca
      481 gttttatgat caaaaattga atattttgcg tcaacaaacg agtaatgaaa cgattattcc
      541 ttgggctagt gattttaccc cagcactttg ttattatcaa ccaaaaaaat ttgctgaaat
      601 aggtgtagat tttgaagaaa tcagttcttg ggaagaatat atccaggtgg caaaagcatt
      661 aaaagaaaaa actggtagtt ttggaattgc cttgccagaa tctggggacc aagaattgtt
      721 tatcaacatg ctagcccagc agaatcaatc tttgcttgat gaagaaggaa atatccgtct
      781 gaatactaaa gaagcgaaac atgctgcaaa gttaatcaaa gaaatgatcg actcagatat
      841 tgtacatttc tacggtgcac aagatgctga gaaagcattt caagaatctg caatgtttgt
      901 tgctggcggc tggtatgcta cgaatatgtc tttgaatttt cctgatgcat ctgacaaatg
      961 gcgcatggct ccactgattc ctttttcaaa agaaaatcca ggaaaaggtg cagtatcagg
     1021 aggatcaagt ttttatgtcc ctaaagatgc aaaaaatcca caagtggcac aacaatttct
     1081 tacatttatg ctgacagatg aagaatgctt agccaacgca ctggagctgg gcgtggctac
     1141 atctaactca ttggcctatc aaacagaagc agctgagaaa aagtttgact actaaggagg
     1201 acaaaaatac tatgaactat tgaataaaat caatgaaaat acatcaaata ctgttttacc
     1261 agccacttat tcagatgcta cgtcttattt gactaccgct gcatataact attggcaaac
     1321 aggtgatttt gaaaattcat atctaaaaga agcagaaaat tttgctcata agtacggcgt
     1381 aaaattaact ggtgattaaa aaatattagg aggacttaac gatgatgcaa tcttcgttac
     1441 caagcaagac aataaagaac agcaagaaaa aagcaacaga tacgtctcat aaaaaaagtg
     1501 cttatggatt ggtcacacct tttgttattt tctttattgt gacgattttg attcccatca
     1561 ttatctcgtt tgcttttagt tttatgaaaa ttggctactc atttgaattt gttggatttg
     1621 acaattatct agctatcttc cgagatccat tgtttattaa atcttacacg aatgttttga
     1681 ttatcatggc aggatcaatt ccaataacga tgctattagc tatttacttt gctgttttat
     1741 tgaattcagc acagttaaaa ggaaaaggat tttttagaac tgtctattat attccaacca
     1801 ttacatcaac ggtggctgta gcttccgtat tcatgacttt tttcaatccg actgggttat
     1861 tcaatggatt tttgaatgcg ctaggaatta acggtgtaca gtggttaaca aatccttttt
     1921 ggatacgaat atcaatgatc atcacgatga tttggcttag tgtaggatat aacactattt
     1981 tatttttagc tggtcttcaa gggatttcta aagagatata cgaaagtgct gaaatagatg
     2041 gagcatcgaa atttcggcaa ttctttttat taacgatccc catgctaaag ccaatcattt
     2101 taatggccac agttttagcc actatcaatg gcttgggtaa tttcaatatc ccaaatatat
     2161 tctttgggac ttccaatgga ccagaaaatt cagctttggt cgtaggagtc aatttatata
     2221 aaacaagttt tgaaatggtt gattttggaa aagcctctgc tattgcctgg acgatggtgt
     2281 ttgtatctgt tatattatcg gctattcaat ttaaattcgg aggtgataaa aatgactaaa
     2341 caaaagatac aaagcagaat ttttacgtac gtcattattg tgtttggtgc cttaatattt
     2401 ttgatgccat atttatatat gattgtagct tcaacgcaaa gtaatcaaga aattttaagt
     2461 ggcggaatca atttcaagtt tggggatatg ctattaaaaa actggcaagc catcatggcg
     2521 agatataatt atcccttgat tatttggaat tcgattgtta ttgctgtaat cagtacagtt
     2581 ttagctacag ccagttctac aatggcgggt tatgcattag caaagtatcg ttttatgggg
     2641 aataaactgt tgttcaatac agttatgcta tctaggatgg tgcctttttt taccacattg
     2701 attcctttat tttacattat gagtcgttta ggattatcga atacttatgc aggtgtgatc
     2761 atcccttcga ttgccagtac aacttcagtg tttatgatgc gacaatatag cagtcaattt
     2821 cctacagcac tgatggaggc tgccagaatc gatggtgcca atgagtgggt gatttttacg
     2881 aaaatcgctg taccgatttt atcacctacg attattacta gtggtttact tatctttatg
     2941 ggaagttgga atcaatatct atttccatta gtcatgcttt cagataaaga taaatatacg
     3001 gttcccttaa ttatcaaaaa tatgacggtt tctagtacag gtgagccaat taattatggc
     3061 gcaattatgc taattttagc aacttcagtg atcccaatgg tcttgattta tgtgtgggcg
     3121 cagtctaaat ttaaggagaa tgacattgga gctgcaaata agtaaaaaaa ataacgaatg
     3181 ttaaccatct agttcgttct aggagcaact ccacattcat aaaaattaaa agaatggcgg
     3241 gatatccatg aatgaacaga cgaaggaatg gtcaagaaaa attttgaatg tagcttctga
     3301 aaagatagat gcggaattag acagaatggg gaataaaatt ccatatattt cttacaacgg
     3361 aaaatataca gacgtaataa aggacagagg aatcgactgg tggaccaatg gtttttggag
     3421 tggtatttta tggcagctct ttcacaagac aaaacgagaa aagttcaaag aggcagcaat
     3481 acaacaagaa caattattag aatctgccct gtaccatttt tcagacgttc atcatgatgt
     3541 aggtttttta ttcttaccat cggctattgc acattatcaa cagtctcaaa acaaacatag
     3601 tcgacaacta gggttacatg cagctacggt acttgctgga cgctataata tttcagggaa
     3661 ctatttagta gcatggaatg accataaacc tggttggatg attattgata gtatgatgaa
     3721 tttaccatta ctgtattggg cttcgaggga aactggtgat ccgaggttct ctaaaattgc
     3781 tcggcagcat gcagatacaa cagctaagta tctagtgagg aaagatggat caaccgggca
     3841 tatcgcagaa tttgatgcag aatcagggaa atatattcgg ttacttggcg ggcaaggata
     3901 caatgagaat tcttgttgga gcagaggaaa tgcatgggct ttatatggat tcgcgttaag
     3961 ttatcgttac acaaaaaatc cgtactatct agctatagca gaaaagatag cagtaaattt
     4021 tattcagaaa atcgaaaaaa ctggttatat tcctctcatt gattttgctg taaaagattc
     4081 gaatattgat accagtgctg gagtatgcgc tgcatgcggt cttctggaat tagcaggata
     4141 tgtttcctct gtacgatcaa aaatgtatca tgaagctgct atcaatatac tccaaaagac
     4201 agaaaagcac tatgcaaatt ggaatatgga tgaagatggg atcattggag gcggagcaga
     4261 agcataccat agagaactga cttataatgt gccactgatc tattctgatt atttttttat
     4321 tgaaggctta cttcgattag aacagtcgga attagctatt tggtagtaaa aagaggagaa
     4381 aatacaatga aacatccagt accactatta aggaccagac aggattactt tgacttattt
     4441 aaaaagataa atcaaccact ccagccgttt tatcgagcaa gtggcgcaca aattgattat
     4501 atacatcaag gggtaggata tggaaataaa attgcaggaa tggaaggatt ctctcgggtt
     4561 ttatggggag cggggcccgc aattgaccaa ttagatgaac gatggttgaa attaattcta
     4621 tcaggaataa aagctggaac agatccttcc catcccgatt attggggaga aatacatgaa
     4681 agagaccagc gtatggtaga aatgcccgca atgttattag cgttatatca tcatgaacaa
     4741 ttattatgga aaaaattaga tacaggagaa aaaagccaga ttattcattg gttctcgcaa
     4801 atttttgaat atgaatgtgc agatggtaat tggcaatttt ttaagataat tgtcggaaca
     4861 attatccaaa aatttggtta tccagttaaa gaatcagcac tgaaagaagc atttgataat
     4921 atagaaaatt gttatcttca aaatggctgg tatcgagaca gttcaagagg tcgccaagat
     4981 tattataatc cttttgcatt tcattattat ggtttgattt atagcgtact agaaccagaa
     5041 aagcccatta gccagatata taagaaccga gctattgatt tttctagaga ctatattcat
     5101 ttttttgcgg aagatggtgc aaatgtgcct tttggtcgaa gcatgattta tcgttttgca
     5161 gtttcaagct tttggtcagc tatgttgtgg gccgatcttt tgccttttaa attaggtgaa
     5221 atcaaggggt tagtcaatcg gaatataaga tggtggatgc aaaaagaaat atttgatgaa
     5281 aaaggtatcc tgaatattgg ttatacctac cctcaattga cattgactga accctataat
     5341 agtacgttat ctccattatg gctgaataag atttttttat tgttggcgct acctgaagac
     5401 catgagtatt ggacggctaa agaaatgcct atgcctattc gtgaaggaac taaacttctt
     5461 tcagaagcca atttgctcat acaacatgat aatggacata catttttttt gaatgcggga
     5521 caattagggc caaatttcca tacattgacc aatgaaaaat atttaaaatt tgcttattct
     5581 agtcaatttg gtttttcgat accgcgagcg aatcaattga aagcagaaat ggcaatggat
     5641 tcaatgctag ggattcagcg agcggacaca tatatcacga ccagttttaa gaatgagagc
     5701 atcaaaacct atggacagtt catcgttcga aataatgtac gagacattgt atgtaagaaa
     5761 gaggtacttg cctctacatg ggaacctact ttacaggcaa aaatcagaac ttggcttatt
     5821 ccttttgaag gctggcagat tcgcgtacac aaagtagaat tagatacaga atatgttcta
     5881 tatgagacag ggtttgctat tgaatgttct cctgagaaag aaggaataat catagaagaa
     5941 gacaaggaaa actatagggt gagtgaagct ggcttttcag gtattatctg tttaagtgac
     6001 gatactatta aacgaaaatc cacagctatt atgggtttgc ccaatacaaa cttgatgaca
     6061 tgggaaaaca cttttattcc tggattagag ggtacatttg gaaaaggaac ccattggctc
     6121 ggaacaggag tggttgctca tcaagatcgg gattatagtt tagaagtatg gaacaatcgt
     6181 cccaaaattg attttgatgg gacaaccggg ctaactatcc aaaataaaaa caataaaaaa
     6241 agactaaaat tatgctaaag gctggtgggg aaatgcttga taaagtgatg tttgaagaga
     6301 taatacatcc agaaaaatgg ataaatgaat gttataaaga attaatggaa aaagaatgga
     6361 ataatgagat cgagcgttgt cgcagacaca cggagaggtt aatcaaacgt atcagtaaca
     6421 gtcagtatca attcccaagc gagtgtccta taaaaggacg ttatccatta agagacaata
     6481 ttggttggac agaaggattt tggacaggaa ttaattggtt gttatttgag aagacgggtg
     6541 atcctttatt tcaacaaaca gctgaaaaag cagatataga ttttatccac aggattgact
     6601 gtcaaattat cactaatcat cacgatcttg gttttttgta ttctttatca accgtagcag
     6661 attataaaat aacgggaaat cgaaaaatga aggaagcttc aattgatgct gcagaaacat
     6721 tacttcagcg tttttgggag aaggcaggag ttatacaagc ttggggagat cctagtgatc
     6781 cttcacaaca aggtagaatg atcatggatt gttgcatgaa tcttccatta ttgtattggg
     6841 cgagtgaaga aactggaaat caaaaatata aaaaagctgc tcaggctcat attcaaaatg
     6901 cagcagatta tttagtaaga gaagatggtt caacttatca tacattttat atggatgtca
     6961 aagatggaaa accaagatat ggaagtacgc atcaaggtta tagtgataat tcctgttggg
     7021 ctagaggcca agcatggggg atttacggtt ttgcattaag ttatcgttat ttgaaagaac
     7081 aaaaatattt tgatttaagc aaaatattag ccaattattt tatgaatcgg ttgccagaag
     7141 ataccatttg ttattgggat ttgatctttg atgattctag cagagaagaa aaagatacat
     7201 ctagtatggt gatcatggtt tgtggattat tagaattaat agaatcaggt ctattagaca
     7261 aaacagaaaa gaacttatac cagtctgctg tttggaaaat tatgcgacaa ttgaaagaaa
     7321 gatatgctac aacggaagaa gaagatggct ttttaacaca gtctgtttat aataagccca
     7381 aaaataatgg ggtaaataca ccttgtattt ggggtgatta ctattattta gaagctttag
     7441 tgagaatgac tagtaattgg tcttcttatt ggtgaaaaag gtaaatatgg aatttttgtt
     7501 tgataaaata aaaaaagaaa aaccaaaaca gcatgaatgg tttctgtttt ggttttttcg
     7561 ttaattttca tattcttcta attccaagct tagttcttcc cattccgtca attttgtttc
     7621 ttgatcttgt ctagcagctt ctaaatcttg gttcagctca ttgagtttta cgtgatcttc
     7681 caggttttct ggcgcgacca tttcttgttc caactgatcg atccattcat cgatccgcgc
     7741 catttcttct tcaacctgag taatttttcg ttgaaggcta cggataagct tttgctgttc
     7801 tttgctttgg taataatcag atttgttcgt gggagcagga gcagattctg gctcatcttg
     7861 tgccaacaat tcggcgattt cttcttcttc ctgctttttc tctaaataat aatcatagtc
     7921 tcctagatag actttgctgc cattttcaga gagttcgacg acttttgtcg cgatccgatt
     7981 gatgaaatat cggtcatggg aaacaaaaag cagtgtgccg tcataatcga tcaatgcatt
     8041 ttctaatact tctttgttgt caatatctaa atggtttgtt ggttcatcca aaatcaaaaa
     8101 attttctttg ttcatcgcta acttggctaa agcaacccgt gctttttcac cgccacttag
     8161 cagagggatt ggtttttcta catcttcgcc agaaaataag aaagcaccta aagcacttcg
     8221 gatctctttt tctggagtgg taggatgctc atcccacaat tctgctagga tcgtcttgtt
     8281 tgtatgcaga tcagcttgac cttgatcgta atagccgatg gtgacatttg tccctagcgt
     8341 ttcttttcca cggataaaag ggatctggtg aatgattgat ttcagcaacg tcgatttccc
     8401 gataccattc ggacctacta gagcaatcgc ttcttgacga cggatatcta gattgattgg
     8461 ttcagataag gtgacattat catatccgat agcggcatct tcgatttgca agactacatt
     8521 tccagaaggc ttttctatac tgaatagaaa gtttgcttgt ttttcatccc cttgaggacg
     8581 atccagccgg tccatttttt ctaatgtctt tctgcggctt tgcgctcgtt ttgtggtaga
     8641 ggcgcgaaca aggttccgag caacgaagtc ttctaattta tggatttctg tttgctgctt
     8701 ttcataagcc ttccattcac ttgctaactg ttctgctttc aaatctagat atttcgaata
     8761 gttacctttg taatggcgca tttttcttct gcttaattca tagacttcat tgactacttt
     8821 atctaaaaaa taacggtcat gggaaacaat caataatgca ccagtatagc tttgcagata
     8881 attctcaagc caggaaagtg tctcgatatc taaatggttc gttggttcat ctagaatcaa
     8941 aatgtcaggg tgttgcaaaa gcatgcgagc taaagctaaa cgtgtgcgct gtccgcctga
     9001 taaagtatcg attttctgat cataaaatga agaatcgaat ttaaacccat gcaatacgga
     9061 acgaatttca ttttcataac catagccgtt tcgatctgaa aaatcgtgtt gcagccggtc
     9121 gtattctttt aaataagaac tataagcagg tgtgtctggc gtaccttcgc taatgaaaat
     9181 ctctagctcg cgcatccgct cttccatttg tctaacttgt tcaaaagctt tcagcatctc
     9241 gttccaaact gtttcattgg aatcaagtcc tgtgtcctga gcgaggtatc cgagagtcgc
     9301 tgttttattt ttagcgatct ttccttcatc aggtgcttca attccagcaa tgatctttaa
     9361 taaagtcgat tttccagcgc catttctgcc aactaaagcg acacgagcgc ctgtactgat
     9421 ttctaaatgt atgttttcaa ataagacttc tgcaccaaaa tggcgagcaa tctgattggc
     9481 ttgtaataat atcatggaat cctcttttct actttgtgaa ttctcctcaa ataaaaaact
     9541 atttttcaat tagtagtcta ccataaattc cattatactg cgagatttgc agaaatgata
     9601 aataaatgaa taaagaataa aatttcacaa tatctgagca atggaattga ttttttccta
     9661 taaatactgc tatgatggag aaatcaaaga tgtaattaga caatacgtgg aggtcatttc
     9721 gtgaaagatc atattattcc aaaggcgaca gctaaaagac tgcctttgta ttatcgctat
     9781 ttaaggattt taaataatgc tggaaagaaa aaagtgtctt caactgaatt aagtgaagcc
     9841 gttcaagtag atagtgcaac gatccgtcga gatttttctt attttggcga attaggaaaa
     9901 cgtgggtatg gttatgatgt agaagatctt atgcactttt ttgcaaagac attaaacgac
     9961 gatcaattga caaatgttgc gctgatcggt gtagggaact tgggaagtgc tttactaaaa
    10021 tacaaattcc atcaaagtaa tagcatacgt atcagttgtg cttttgatgt gaatgaggaa
    10081 atcgttgggc gcatcgtaga cggtatcccc gtttatccaa tggaagatat gtcagaacaa
    10141 atcaagatcc aacaaatcga agtagcaatt ttaacgatac ccgctgaaag agcacaagaa
    10201 gtagtcaatc agttaacgga agcaggaatc aaaggaatct tgaactttac cgctgctcga
    10261 ttgaatgctc cgtcagatgt attgatccaa aatgttgatt tgacgaatga attgcaaacc
    10321 ttgatctatt tcttgcattc tgacgcaaat gcgcattccg aaaatacagc agaataaaaa
    10381 taaaaagcga ttgaacaaat gaaggcaaaa ctttcacttg ttcaatcgct tttttctgtt
    10441 tggacaacga ttattttgtt cggaagattt cttcataaga aagggaatca tcttctgctg
    10501 tcagttcatc tagtagataa cggatttttt taaagtcgat gaaccgtgct tgccgaaaga
    10561 cttcttttcc ttgatgaaaa atcaggacaa ctggcgcagt cagcacttca aatttactag
    10621 ctacttctgg tgtttcaaat gcatccaaat gaagtgccgg aatatcataa tgtgtgagaa
    10681 gttcctctag tcgtggacga acagcgtgac aaacactgca attaggcatc gaaagataaa
    10741 tgacggcaac ggatgactgt tgcagtatgt cttgaacttc agtcaagtta gtggtttgtt
    10801 tcatttttat tctccctttt atgatagaag tgatatgtat gagaccttaa tttaatggga
    10861 ataacagaaa aagtcaattg aagtgctttg gtaaaaaata agagttgtgt aaaaatttac
    10921 agagcaactt gttttttttg aataaaagcg tatatttgca aagcgaatga actagaaata
    10981 aaaaggagga aactcatgtt ttcttttatt ccaaattcgg ttttgcagat gagtacgatt
    11041 ttcttatcga tcgttattga agcattacct ttcgtattat taggctgtct tatctcaggc
    11101 gccttacaag tatttttgac cccagaacgt gtgaagagat ggcttcctga aaatcgtatc
    11161 ttctctattt ttactggttc gatcttagga tttttctttc cttcatgtga atgcggcatc
    11221 gtaccgatcg ttcatcaatt tgttaaaaaa ggcgtaccag ttcacacagc gtttgctttt
    11281 atgctgactg ctccgatcat caatccaatc gtgatttttt ccacttttat cgcatttggc
    11341 aattcttggc agatggcaag ctggcgggtg ataggcagct tttttgttgc ccttgtgatt
    11401 ggtgtctggc ttgcctattt tcaaaaagac gctgtattga aagaaaaaat cacgtctaaa
    11461 gtagcagaaa atcaccatca tcatgtccat gaagaagaac aaagtgagaa gaatgaacca
    11521 aaaaatcagc tgatccatca aactgggcat gtgttgacac attcaatcga cgaatttttt
    11581 gatacaggtc gttatttgat cattggcgga ttaatcgcag ctgcgatgca gacttattta
    11641 ccaactagaa ttatgctaac gctcggctca acaaagattt tagccattat catcatgctg
    11701 ttattggcct ttacgatgtc tctttgctca gaagcagatg cgtttatagg atcttctttg
    11761 ttaagtctgt tcggcacagc acctgtagct gcctttttgg tctttggccc aatggtcgat
    11821 atcaaaaatc tactgatgat gaaacgatat tttacaggac gctttatttt atcgctgatc
    11881 ggactgattg cccttagcgt tattggattc acattattta tttaggaggg aaaatatgct
    11941 gcggtttttg attttatctg ggtatttcga attgatgatg tacttgcaag tttctggcaa
    12001 actcgatcaa tatatcaatg tccattatcg ctacttagct attctttcaa tgattttatc
    12061 tgctcttttg gctttagttc agctgtattt gtgggtcaaa aacggaaaag cagaagaaaa
    12121 ggcacacgag cacgatcatg accatgggtt gtcaaaacct tatcaacgtg gaatcgctta
    12181 tgtactgcta gctttgccag taatcgtggg gacgttgttt ccaacagtta gtctcgacac
    12241 tacgatcgta gaagccaaag gatttaattt cccagtaagt aaagaatcag taggggatcc
    12301 ggagatgcag acacaatact taaagccgga taccagtatt tatttcaaca aatcagacta
    12361 cttagaccaa atgaatgagt tgaaagaaaa gtatggggat aagcaaacta tcaagatcac
    12421 agatgaaaac tatttagaag ttatggagct aatttataat taccctagcg aattcattgg
    12481 caaaaaaatc agctatgaag gatttgttta ccaaacaccg aacgatagca atacagatat
    12541 cttcttgttc cgattcggca ttatccattg tgtggcagat tcaggagtct ttggtttgtt
    12601 gacgcatatg ccggaaggag taacggttaa aaatgatgaa tggtataaaa tagaaggaac
    12661 catccaatca gactattatc aaccattcaa gcgtgaaatc ccatctgtag ttgttacaaa
    12721 cgctgagaaa gtctctgctc cgaaaaatca gtatgtgtat cgttcttttt aaaagaacaa
    12781 aataaataaa catagctcag acagctgaca cgcaaggacg tcggttgtct ggcttttttt
    12841 gcatgatgta ttcatttgtt tgatatttat tttagaagca gcatcctgaa agtatttgat
    12901 atcggtttca aaggtgttgg aaaagattgt gaagagaatt acaatttatt cctaacatgt
    12961 atgttgaaaa gttgtatgaa atgattttga tagtaaaata tcgagtggag gtacttttta
    13021 tggtaacgcg tatatttatt ttggatgata gtttggtttt tgaaaaaatg ctagtagaaa
    13081 ttttggaaga atccagaatt ttattaagtc cttggaaatt tgagatcgtt cagggattga
    13141 acgttataca atttgttgaa ttcattaaaa ataatgaaat ctattcctca gatatttttt
    13201 tcctagacat cgatttgaac gcctattata gcggtatcga actaggcgaa atgcttagag
    13261 aaaagaatga aaagtgtaaa attgtttatc taacgtctca tccgaataaa gcaataactg
    13321 ttattaatcg aaagattacg cctagtggtt atttgataaa agaattcact tatgaaaaaa
    13381 ataaaaaaag catccaaagt tacttattga aagaaaaaga aaactatcct gttcaacatg
    13441 ctacgaaaaa aaggtggaca actgtccgtt atggtaatag cgatcactat atccattacg
    13501 atgaaatcct ttatctaatg agcatgccag gttataaaaa taaaataggc ctagtattga
    13561 aagatgaaga gattctcgta aacggcacaa ttaaaaaata taaaaaatca ctcgaatatg
    13621 agtatttatg taaagattta aaagcttata tcattaatac tgaaaacatt acatctatta
    13681 gcagagtaaa cgaaatagta acatttgcaa atggagaaca actagagatg ggaaagagaa
    13741 gtattgataa actaaagaat tttatgcaag aaaagataga aaatgaatca ttttaaagaa
    13801 aacaaagact catatcttac ctgttagaag agtgttttcc tgtagaagag aacaacccaa
    13861 gtagcagcct agtcgtttct atcctttttt gttatgcact ctttttgaca tttactccac
    13921 caataacgaa gggtacaata aaaagtaata acaaacaaaa ggagtacaga aatgattgaa
    13981 ttcatcaatg taagtaaaag ttacgggaaa aaacaagcat taaaagactt gtctctctcg
    14041 ataagacaag gggaaatttt tggattttta ggacataatg gtgccggaaa atcaactacg
    14101 attaaaagtt tagttagtat tattgaacca tcatcgggaa cgattttagt agatggaatg
    14161 aaattgacgg aaaatcgatt gagtatcaaa caaaaaatcg gttatgtgcc tgattcccca
    14221 gatatttttt tacaattgac ggctggagaa tattgggact tgatttcagc tgcctatgaa
    14281 ttagacacac aaaaaaaaga aaagcgttta gcagaattga cagcgctttt tgatatgtat
    14341 agtcatcaaa atgaaacaat cgcaagtttt tcacatggaa tgcgtcaaaa aacgattttg
    14401 atcggcacgt tgctgccaga tccagatatt tgggtattgg atgaaccttt gcaaggactt
    14461 gatcctcaag cagcatttga tttgaaagaa atgatgaagg ctcatgccgc aaaaggaaaa
    14521 acagtcattt tttctacaca tgtattggat accgcacaac agttatgcga taaactggct
    14581 attttgaaga aaggtgaatt gatttatcag ggggcagtta gtgaattatt gaatggctca
    14641 cctgatgaaa cgctagaaaa gatttatttg aaaatggctg gaaggcaagc gacaggagat
    14701 ggaccgtatg tataagcgac aattattaga attaattcga gttaatcttt tatatgttaa
    14761 tccacagctc acgaataaag caagagagaa aggaaaaaag ggaaagcgat taactatcta
    14821 tcttttgtct caaaatcttt tttcagggct gattttttta ctgatttacg gatttgctat
    14881 gtttgctatg aactttgtta aattgccagg tttctttact tattacgtgg cactttttgg
    14941 tatgttaggt ttaactcaaa gtatatcagt catttataat atcttttttg aaggaaatga
    15001 cctgcaagta tttttgcctc tgccttttgg acaagaacag gttttttcag caaagatttt
    15061 ggtagttgct ttgaccatca ttccttttgt ttttccgctt ctagtcatgt ttattttaac
    15121 cggatggcga gctgatctag ggattttgct tactatcatt atctcaatcc ttttgttttc
    15181 tctatttttg attattcttt tttgtctggc tagtttgatt gtttttggtt tggctcgaac
    15241 agcagtgttt aaaaaacata gaaaaacagt ttctagtatc ttgcttggca gctcgatgct
    15301 gatcgcattt gctggaatca tgtggatgaa tcatcaaacg gggactattg aaaatgagtt
    15361 aacagatcgg catccgattt cgatattatt gccattctat gatgctgcca gtcagccact
    15421 ttctcaacaa ggtttattgg gttggggaat actagctatc acggctctgg gatttttcct
    15481 tgctgtaaaa cagctgatag taccaaagct tacagagcaa ctgactgatt caaacagtga
    15541 gttcaaaata aaaagaaccc ataaaaagaa ccaaaatgtg catcagcttt tatttagtta
    15601 caatgctcaa ttagtaaaaa aacctaattt gattatgcaa gttctttctt cttccttgtt
    15661 aactccaatc atttttattc ttgcttttgc cctaggaggc gaaataaaat taactgattt
    15721 agataataag ttaatcggtg ttgttttcct ggtagggatc gcattggctt tcttaacagt
    15781 taatcagact tcttttatca gcaatcttat ttctttggat caagaaaatt ttctgtttat
    15841 ccgttctttg ccaatctcta tgaaccaata tttaaaagaa aagttcagat tcggatggat
    15901 attgcagagt tttttaactg gtgggatcgc attattaagc ggactgtctt ttcagctgcc
    15961 tttgtttttt atccctagct tgctatttgg ttctttattc ggctgctact tattgtcact
    16021 acgttatttc gctagagatt atcggttgct gctgctgaac tggaccaata ttaaccaatt
    16081 atttaacaga ggttcaggaa gtttgggttt agtcgcaact atgatgggtt cattgatcat
    16141 cagtattatt cttatcgtct tatacggaat gttggctttg ttttatccat tttggttagt
    16201 gaatattcct gtgatcggtt tagttctaat actttccgct ctatggatcg tttattatca
    16261 acgaacgttt tggaaaaaat ttgaataaag aagaggggcc gtgacaaaaa tcatgtcgca
    16321 gccccttttt tacagataga gggtgttcaa aagtcagttt ctcagtatta agtcaatttt
    16381 atgtaaaaat gtgagaagct atttttacaa aacttttttt aatactcata attacttctt
    16441 attgctcctg cggttacttg tcgcaatgtt tgtgagatat tttgtcatcg ctcaaggctg
    16501 aacgtctctt tcacaacctt ttctttgtta cacttcgttt atttaaaaag ccgttttttt
    16561 cgtttcatca tgattgcgtc acgctttact ttgaaattac ggatgttatg gttaaagact
    16621 tctgataata caagacctaa agcgattgct ccagcaatca taattgcttg gaccgcataa
    16681 tgagccgctt ctcgatagga ttcagtaacg agatttcgaa ccgcttgata agctaaacca
    16741 ccagggacta gaggaaccat cccgggaata ttgaaaatcg ttacgggcat tttcaaacgt
    16801 ttagagaaaa gatcgctgat gaatgctact cctaaagaac caagcaatga accaaaagca
    16861 gcagatccgc ctatttgaac acagatccag tagatcatcc agccaaaagc accagaaaga
    16921 ccacaacaaa tcaatgaacg cctaggtaca tttgtaataa cggcaaatgc cgcagtcgct
    16981 aaaaaactaa aagaaaactg aataaatatc gtccccatac ttgtgttcct ttcctaataa
    17041 aaaagctgga aaataaaagc aatcgcaaaa ccgatcatcg tagcagtcat catcgcttct
    17101 gttccgcgag aaactcctga gacataatgt ccagctagca aatcacggat agcgttcgta
    17161 atttgaactc caggaactaa aggcatcaca catccgataa tgattagatc ctgattgctt
    17221 cctaagccaa aacgtacgct gtagatagcg gctatgccaa tcaataaaga agataaaaat
    17281 tccgataaaa atttgattct aaaaaatttc aaactatata gataagctgc gtatcccaat
    17341 cctccaatga ctaaagtcac aggtaaatcg gtcatctttc cgccaaataa caccataatt
    17401 gttccactga tcactgccgc gctaacgatt tgcaaccaga caggaaagaa agaacggtct
    17461 cggtcgattg cttgcagttc gcagtaaagt tcttctaatg ttagctctga agcaacatat
    17521 ttacgtgaaa gatcatttac ttttactact ttttctaaat taatcgaacg gttggtaatt
    17581 tgttccattc ggatggtcga ggtacgatca agaccaataa agagaccggt ttgagtgaca
    17641 tagctgacca aacggaaatt tccagaagct aaagcaattc gactcatcgt atcttctacc
    17701 cgatacatct ccgcattgct ttccatcatg attttcccag cgagtaaaca agtgtccagc
    17761 aacagatcta attcttttcc cgtcatagca tcttctccaa taattgattg attagtttta
    17821 ttttactaca aaacatacca gaagcgtaaa gaataaaaaa acttgaggaa ggaataaaag
    17881 aggcgtatac tgtttttgta ttgcgggtgt agtttagtgg taaaatccca gcttcccaag
    17941 ctgatgtcgc gggttcgatt cccgtcaccc gcttaaaata ggttgtgaaa aagctgctct
    18001 gcaacaagta ataagaacag ccaagcaaaa acaacttctt atgtttttaa cttggatgga
    18061 cttattaccg cagatgcaag cttttgcgca ccgtttacca agcggttgtg aaatcagtgt
    18121 attgtcttga gtatgaagaa aatttttgaa taataataaa taccttacag atagagtggt
    18181 gactgattag agacgaggaa tccgtctctt ttttgtattt ttaagccatc ttaaaacagg
    18241 ttttattttt tgctgtttct atttctttct agtatttatt tataggggaa catatgttaa
    18301 gatttaggga gctgttagta aaggagtaaa gaaatgagca aaaaaacaac caaggcgatc
    18361 gtggggatcg tcgctgctgt gattgcaggg gcattcggtg tttccaccac acaaaaagat
    18421 tcagatatct ttcagcagat tacaactatt tttgattcca caccacaagc ttctcaagta
    18481 gcggatgtgc cagaatatga tggaaaacat caagaaatag aaatcaatgg gaatcagccg
    18541 gaatttacaa aagaggatct tagcctttcc aaaggaacat gggaaagcta ttcaaatatt
    18601 gataaattga acagagtagg acaagccaat gctatgcttg gaaaagattt gttccctact
    18661 gaaaagagag agcctttgta tattgacccg acaggttgga aacaaaagaa attaagtgac
    18721 gggcaatggt tatataatcg cagccattta attggtttcc agctgactgg tcaaaataac
    18781 aacattaaaa acttgatgac agggacacgt tcgttgaatg ctccatacat gcttgctcac
    18841 gaaaatgata ttgcggcata tatcaaagaa acaaatcatc atgtacgcta ccgtgtcact
    18901 cctcattttg aggggaatga actggtagcc aggggagtgc aattggaagc agaaagcatt
    18961 gaagacaaaa aaattgaatt caatgtcttt atctacaatg ttcaagatgg ctacacgatc
    19021 aattatgaga caggacaagc aacaaaaaca aaaaattagt agcagctatt cgaatttttt
    19081 atgaatatta tgaaaaaatt gtgtaaaaac atacattcaa agaaatatag cgtatacttt
    19141 atgaaaagag tacttttgat aaagctacag aaaaaaggaa aaaaacatac aaatagtgtt
    19201 tcttaattta ttcggaagtt tggaatattt gtttgtagtt ttccctttct tcgagtaaaa
    19261 taaggttgta gaaggtaagc tggaattacc ttgcgggaaa gaggtgtaga atcatgggtt
    19321 ttacagatga aaccgtacgt tttggttttg acgatagtcg caaaaaagaa gtgagcgaaa
    19381 cattagcgat tgtttatcgg gctttggaag aaaaaggcta caatccaatc aatcagattg
    19441 tcggctactt gctttctgga gacccgtcct acattcctcg ttatcgtgat gctcgaaatt
    19501 tgattcgccg tcacgaacgc gatgagatca tggaagtaat tgtcaaggac tacctctcaa
    19561 atcatggagt gaatttatga gagtaatggg cttggatgtc ggttctaaga cagtaggtgt
    19621 tgctatcagt gacccctttg gctggacagc tcaaggagta gagatcattc ggatcaatga
    19681 agcagaagaa gagtttggct ttgatcggtt gaaggaatta gtagcacaat atgaagtaga
    19741 ccggtttgta gttggattac ctaaaaatat gaacaattcc atcggaccaa gggcagaagc
    19801 ttccatgtca tacggagaaa agatcaaaga actattctcc ttgccagttg attatcaaga
    19861 tgaacgatta accactgttc aggcagaacg tatgctagta gagcaggcaa atacttcaag
    19921 agccaaacga aaaaaagtca tcgataaatt agcagctgtg atgatcttac aaaattattt
    19981 agacggctca ggtaagcaat tattttaata ataaaagaaa agaggtaatc ttatgacaga
    20041 acataaccat tcccacgacc atgaaggaca cgaacacatc acattggtgg atgaccaagg
    20101 aaatgaaaca ttatatgaaa tcctactaac agttgacgga caagaagaat ttggacgtaa
    20161 ctatgttctt ttatatcctg ctggtgtttc agaagatgaa gatgtagaat tacaagctta
    20221 tgcatacatc gaaaacgaag atggaacaga aggcgagtta gaacaaatcg aaacagacgc
    20281 agaatgggac atgattgaag aagtattcaa tacattcatg gcggaagaag aagaatagac
    20341 tataaagcta ggagaaacct tgatatgata gggtttctcc tttttttatt ttttactgta
    20401 tgccatattg tatgccatct agtcaatatt taagtgttgt actaattttt caacagtctt
    20461 ttctttcgtt tcttttgcca agtgaccgta acaatcgatc gtctgactaa tttttgtatg
    20521 ccgtaatcta gtttgaactt ctaccatagt agctccagcg tccaagagca ttgaagcatg
    20581 agtgtgtctt gctacatgca caccgagaaa tggaaccata gctttttcac aaatagtaat
    20641 caatcttgca taaatagcgt ggtgtgtgat tagttcttta tagataccac aaaaaatcat
    20701 agtcggatca gttacaccaa gtgtcagcat atatttccgc tgattaatct gccatttttt
    20761 taatatagaa attgttcttt catctagtgg aagaacccca gcactacaat cagttttagg
    20821 atcactgatt ttatagccat ttttggattg gcttagtgtt ttattgatgg tgaccgtatt
    20881 ttcttgaaaa tcaatgtctg accaattcaa tgcaagggct tcactgatcc taaccccgct
    20941 ataaaagaga aagcgtagta acgttttatc aaagtcgttt cgataagttt caggtttgct
    21001 gtttaggtaa tcaaacaagg tgatcagttg ttctttactg taaactttga ccttttcttt
    21061 atgcttagtg gtggttcttt taggcattaa tacgttgtct agtggattag tctcaataat
    21121 cccaagattg atagcgtagc taaaaatacg cttagaagtt gaatgaagta ccttgtattg
    21181 gtcagtagtt tcagcccagc tatttacaat ttgttgggct ttttttaccg tgacttcatt
    21241 cacacgaaga tttccatatt ttggaagaat ccactttcta aacttgattt cagttttcat
    21301 gaaggtggct tctcggacag tggttttata tgtttctagc cacaagtcat aaacttcttt
    21361 aaacgtttcg ttgttcgctt ttttaatacc gtttttatcg tagtccactt gcaagcgtga
    21421 caaggcaagc tgggcttctt tttttgtttt gtaacctcgt ctacgaacct tatcaggttt
    21481 gccagtcatt ttgttcattc caagataggc ttcatagagc caaaatttac cttttttagt
    21541 ttcatattgc ttgaatgttg ccataatagt tttccctcca tagtgtagca agccgtgggg
    21601 gcggttggag gaggcagttt attattattt tgaatagtgt ttatttagct cagtaacaag
    21661 agctgtgaac tcttttagaa gatcattttc ataaaattct tgatactctt ttttctcatc
    21721 tgtatttttt tctgaatcat taattaatga gtcatatcgt aaaagcgtat ttagaagagc
    21781 tgcgagataa gcaattgggg tactcccgtc aggaaattca attcgagatc ctgttttaag
    21841 atattttatt gcgtttgtta aataaatctt ctgtgagaca gtcaggttat cttcttcgat
    21901 gttattgata gcattagaaa gattagaatc aattatagag ttattttttt caatagtttt
    21961 tttcaattgc ttttcaagca catttcgctt aatttcgggc atcatatgca tatctcttaa
    22021 tgtataagat ccgtttaata gataggtagt ggatacatct aatacctttg aaagttgttt
    22081 aagcctggtg ttattgggaa gattatagcc attttcccaa ttactaacgg cacctttact
    22141 ggccggtggg gataatttac ctccaagttg ttccattgag agtccctttt caattctagc
    22201 cagtctaatt ctttgtcctg tctccactgc ttcggggtat ttttttctag ccacattttc
    22261 accaccattc atatattttc tctactatat cacgatttaa atatgtgtga aacataaaag
    22321 ttcaaaaaag tatgttaaaa ttcttgcaaa tgaaaaggta tgatggtata ttgagtttat
    22381 aagaggtata gaaatgtatt caagagcctc ttgcattttt ttaaaatctt tgtatagaaa
    22441 tgtatgctaa cgagggggga taaatatgga attaaaaatt gacaaactca cgcttgatga
    22501 tccagctttt aaaattattg acgatcgagt tgttcaacaa gtagagcaaa ctcaaggaac
    22561 gttactttca ttattagaaa aaaagtacca gtttccaaaa tatatgaaca aggcacaagc
    22621 tgctaagtat atgaacgttt catacaatac tatgatgcag aaatatgttc ctaacggttt
    22681 aaagttgatt atagttgatg gagtcgtaag aatttcacaa gatgaatgtg atcgctttat
    22741 ggaagaatac caaaaataaa cgcaagccgt gggggcaaag ggattaatag gaggaaataa
    22801 taatggaatc gacacaaaat aaagcaatcg aaaaagtatt atcaacaatt cttacagaag
    22861 acacagcggc agaattagca aacttggaag gcaaagcgct agaagaaacg tttgaatgtt
    22921 tgtacgaaca aatggactat caaaaattgc taccacaaga accaacggta agcggagtat
    22981 tgcgtggctt gaacgattta gtacaagcag agtttaaaga acgactttct attgaagagt
    23041 atcaagaaat tttgtatcag caagttgagt tgttagcgag cttgctaggg cttgaactgg
    23101 aggacgttga atgaaaactg aaatttggaa cggacacatt ataagatttg tcgatattaa
    23161 tgatgaatgg tgggcagtgg cgaaagatgt tgcggaagcg ttaggtctta aacaggttac
    23221 tagagccatt cactctttgc ctaaagatgg ggttactact agtaaggtca tcgattcatt
    23281 gggcagaaca caagatgtaa acatcatcaa cgaaaaaaaa tatttaccgc atggcattca
    23341 aaagccgtaa aaaagaagcc gaggcatttc aagactggat tttcgacatc atcaaagaac
    23401 tacgccagtc cacaggactt gaaggctttc aagtattccg catgctagac aaagaacacc
    23461 aaaaagaagc aatgactaaa ttaagccacg ctattactga accaaaacca gtagactaca
    23521 ttaaagcgaa tgtgatagcc aataaagctg tatcaactat ttatggtcat tccaaaatgg
    23581 taaaaaagaa agatatgact cctgaaatgt tggttgatcg tgaacctatt ttagatgaga
    23641 cagtagaatt aatgactgtt aaagaaaaat atgggttgca gttttcagtg agtgagaaaa
    23701 tttataatcg ttctgctgaa ttgcagacaa cttaggagca ttaacatgat cgagaacaaa
    23761 gaaaaagcct tatccataac agtcttggcg gacaggataa aggcaactta attgggataa
    23821 aacccgatat tctttgttcc gattatacca tattaaaaaa tcggagggaa ccattatgaa
    23881 acgaaactta attataggaa gcattttaat cggcggaatt ttcggcttat taccattaat
    23941 tgcaaaaata gctatctttg caggattagc gttatatctc ttcactgctt atgacgagta
    24001 cgactatcaa ttaagaactg gagggcataa gtagatgtac aaaacaaaac tattaaacca
    24061 actcgatagc cttgaattag aggaaattaa tcaagggatc gctgaactag aaaataatat
    24121 cgggaaaacc tactttggaa attcattcaa tgaaaaacta acagttttgt atgtactcaa
    24181 aaaacatgca gaacacaaaa taatttgtcg agaaatcaac gagctaaaaa atcagatttt
    24241 aaccgcatgg ttaaacatta ctgatatgca agaagcaagg gtgaaaacat ttaatacatg
    24301 ggtaaagtat cagaatcaat taaaaggtgc tgaatttgtt cgggatgggt tgaaatacga
    24361 acttgagcag ttgaagctca tggaggtgtc tgaatgattt ataccagtat tttgaaaaag
    24421 gaggagaggt ttattggcaa ataattatca agcagcaacg gaattacaaa agtcgggatg
    24481 gtcaatttat cccttatctc ctggaactaa aattccaata aaaggctcta gtggttggaa
    24541 tgatgcaact aacgattcta atcaaattaa tgaatggtgg acggaagatg caactagaaa
    24601 tattgggtta atgcttgaag atcatcaaat ggtggtagtt gacctagatc aacattctga
    24661 aacacgaaac ggcgttgaga attataaaaa acttgcttca atgtatgatc catttccttc
    24721 tacttatacg gaaatcacac caggtaaagg aatccatttc ttctttaaaa ttcctgatgg
    24781 tgtaattatc caacaagaaa caggtgcatt tgctgatatc ttcgggcgtg atgataaagg
    24841 taaaagttta agcgggattg atattatcac taaaggagtt ccaatagcgc caactatgat
    24901 tatttcaaga agtgttggga gagcatatga acctattgaa aacgtatctc taaataatat
    24961 tgcaccagct cccgattggc tagtgaacct actgctaaaa aaaccgcaag aaataaagac
    25021 tcaataccga cccgtagcaa aaacagggac agcaaaaaaa ttagatatga ttgttcaagg
    25081 cgctggcgaa ggtagtagaa acgatcattt aactaagtta tgcggttggt tactatggca
    25141 cggtgtagat aatcaaactt tagcagaatt gatttataca gcaaatgcgt acaatgaacc
    25201 accgctaaaa gataaagaag tgaatcaaat tattcgatcg atgattaaaa aagatttgag
    25261 ggggaaagct catggctcat aagcataaat tagatagtat tttagacttt cccgaagcca
    25321 gcgagcgtga agacaatatc attgaattga aaacctggat gtcacgatta agatgcaata
    25381 aagacgatca gattaaaagt aacagtgttg tgaatgcaga attgatttta accaatgata
    25441 gcaatttagc tggaacaatt gcttataacg aatttagcgg atacattcac ctattaaaag
    25501 attctccttg gatcaatcgt accgctggag aatgggaaga cagttttgag gatgccttaa
    25561 cggcatatat tgaagaaaac tacaatgtag tttttgatga caacaaaata cacaaagcag
    25621 tagtaaatgt ggctagaaag aatgttttca acccagtaaa agaacgtatc gaaaaagtta
    25681 aatgggatca acagcctaga ctggaaacac tgtttattga ccttttaggg gtagacgaca
    25741 acctttacac acgagaagta acaaaacgtt ggatagtcgg atcagtcgca cgtatctata
    25801 aaccaggaat aaagtttgag atcgttccag ttttggatgg accgcaaggg attggtaaga
    25861 gtaccgttcc agcattactt tacacggatg attttttcac cgattcatta gattcgctag
    25921 gtgaaaagaa agatgattat atgcagcttc aaggcaatgt aattattgaa cttggagaat
    25981 tatcttcaat gaacaaaact aaaattgaac agataaaaaa cttcatcagt gccaagatcg
    26041 ataaaattag acctccttat ggtcggaatg tcatagcttg ggcaaggcaa tgtgtattta
    26101 ttggaacgtc gaatgatggg cagtatttga aagatgatac aggaaaccgc cgtttttatc
    26161 ctttgccttg taagaataag ccaaagatga atccgtttaa aacaaaggat gaatattttc
    26221 ttcaagtgtt agctgaagca aaggtgcttt ttgataaagg acaacgaatc tattttagtc
    26281 ccgatgaaga taaagaagtc ttagaaattg caaaagacta tcaagaggat gccaagatgg
    26341 agaatccaat taaagaagca atcactaaat atttggaaat ggaaatcccg tataaatggg
    26401 aagaagctcc agcgtgggcg agacgttctt attatcagaa ctatcctgac agtaaatgta
    26461 atgagaaaaa tttacagcat ttctataatg gagctactat gcaacaagag ttttatttag
    26521 ttgatagcgt actcaccgct gatattttgg aagctgtttt tgataaacaa gcaaaggatt
    26581 tattgaatgg tcgatcagat gcagaaacta aaaagattgc cttgattatt agcagtattc
    26641 ctggttggga acggaagcaa ctgccaaaaa gaaataaacg acgaggattt tacaataagt
    26701 caaatgccta tgaaaataaa aatcgagctg gaaagtacaa aaagtaacgg gtagtaacgg
    26761 gtcagtaaca cctagcccgt tactagttta cccttactgc cccaagggat acagcaattt
    26821 ggtaacgggg taacacctta tttccttaaa acttttgaaa tagttagcta atatgctggg
    26881 gaataatgtt actggatttt acccgttacc tgttaccaaa acgctagaac ccttgtcccc
    26941 gttgggattg agtggtaacg ggtacctgtt acgtaggtgt taccacgtgt tacgaatcaa
    27001 ggaggaaagt aaatgctcaa tattatttca acaaacaaag caccgaactt tcaatataca
    27061 gatgaaatgg atcggtttct aatgaacacg ctggcattta gtgtgggatt ggtaacagaa
    27121 gattattcca catttgatcc agaagtatta aagataatgg aagaagaacc tgattggctg
    27181 caggagtcag tagcgtggtg tcaatcgcta gtagtgggat cattggtaga tagtggcaac
    27241 tatgatgata caggtgagct aatggatgaa tttaattgtc tgcttaacct atatgatcga
    27301 gcgagacaac gagagcttac atcgaatgag gataatttgt ttttaaacat ccatgataag
    27361 ttcttggctt tgctactaac agatgatgaa ttgataacta atttattgga ggtagaataa
    27421 ttatgcatat ggaaagtatt agatacttcg atggtgagaa acacattgag tattcaaaca
    27481 ctcaagaaga tgtagacttt attagttttg aggaagataa aatcactaaa gttaatttta
    27541 aagacggaac gtgcttgaag atagtttcgc cttatattga atacaaatca aaatgggaac
    27601 aagatgattc tgacgaacga gatcgctttg gttggtaatc tatggcagtt aaaaaacaat
    27661 gtaatcatgc tggatgtaaa acattgattg attataggca gaagtattgt gggaagcata
    27721 aagcaaagca aacggcaatt aagcgtgaag aaagaaagca aagcgaaggt aaatattttc
    27781 aattctatca aagtagaaca tggcgaaagg cttcatactt gtatcggtta aatcatccag
    27841 tatgtgagga ttgcctagaa gaaggcttga taaggaaagc tgatgttgta gatcacaaaa
    27901 ttgaactaaa agatgattgg aatagacggt tagatgaaac taattttcgt tctttatgcc
    27961 atgcacacca taattccaag acagccaatg aaagacaaag acgagaaaag agtacccttt
    28021 gagttaaggg ggcatagtgt gaaacttact gacaatcgat gcctactcat tttggtacaa
    28081 ataaccgttg tgaaaaggca taagggtaat tacaaataac gattacactt gtaatcacaa
    28141 gtgaaaaagt atataatgaa agtaggagat ttattgaaaa atacaattgt tattactgat
    28201 aaaacaggga aacagcgcac cattgagtta cctcattttg gtcaagtgac cattcaaatg
    28261 cagaacggaa aaatcattta tatagataaa ttagataaag aaaaattctg atcgaaaaac
    28321 gaaggaatgt gagcttaatt gcttacgttc ctttttcttt tgtctgaaag gaggacaaca
    28381 tgggacaccc taaaatatta cacgatacaa aaggcaatat atcaagtgaa gaaaaagcaa
    28441 tccgtgtgga cgctagagaa gaattgttca agcagcaacc attaataaat atcacgcccc
    28501 ctgactggat ggcagcgagc gctagaagtg aatggaatcg tatcgtacca acattaaaaa
    28561 aagattatcc attgagtgaa gcagattatg gttcattggt agcctattgt ttagcctttg
    28621 ctcgaatgaa aacagccgaa gccgagataa gaaaatcagg tacgtttatc acatgcgaaa
    28681 acggagtaaa gaaagccaat ccagcggttc gggttcaatc acaagctatg agtgatttga
    28741 aaaaacaagc cacctcacta ggtatgacct tagaatcacg atcaaaacta gctttgaaca
    28801 aggttaaaaa tgatgaaccc gaagacccgt tcaaagagtt gatgaattca tgaatgatta
    28861 cattgagaaa gtcctatcag gtaagctgat tgcacccaag aaaattatcc aagcgtgtga
    28921 gcgtcatata agcgatttgg agcgttctaa gtcagatagc ttcccttatg tgttcgatga
    28981 agaacaagcc accaaagcga ttaaattcat tgagttgcta ccatctacgg acggtaaagc
    29041 aatcaagatg ttaggatttc aaaaattcat tctaggcagt ctttatggct ggcgtactaa
    29101 agaagggaat tacaggcgat tcaatcgagc gtttaccagt atgagccgta agaatgggaa
    29161 aacgtatatc gcaagtggca tggctgccaa tgcgttgatt atggaacaag aaccagcaga
    29221 agcaaggcaa gtattgtttg taagtaacgc tttgaaacaa gctaaattgg gctatgatat
    29281 gctgtctaat tcacttagaa acgtggtcaa gtctagtaag tttttaagac cacaactgaa
    29341 aattatgaac tctaagattc agcacttgcc ctctaattcg ttcgctatgg cactggctag
    29401 tgaaaccagc acgctagatg ggtttgcacc aacaaccgca attcttgacg agtggcacga
    29461 agcaaaaact cgtaaaacgt acaacgtcat taagtcagga atgacccaac aaaagaacgg
    29521 cttattgtgt gttattagta ccgctgggct tgatttaaac gttcctatgt acgaggaata
    29581 cttattgtta gatcgtgtgc taaaaggcga agaacaagcc gacaggtact ttatagcgat
    29641 atgggaattg gacgatcccg aagaaattca cgatcaagag aaatggatca aagccaatcc
    29701 gatttttgaa agtgaagaaa tcaaaaaagt aatgattcca accattcaag atgatgtgaa
    29761 ccttgctttg aaacaaaata accttaattc tgtattggtg aaaaacttca atttatggag
    29821 acaagcgagt gaggacagct atatgattgc tgaagactgg caggcaaccg aagtagaacc
    29881 acaagatatt acaggcaagc ccgtttatat cggagtggat ttatctaaaa cagatgattt
    29941 aactagcgtt tcatggatcg taccgctaga taacggcgaa ctttattgtg attcacatag
    30001 ctttgtagcc accaaatatg ggcttcaaga caaagaaaag cgcgacggtt tgccttatcg
    30061 agaactagaa aaagctggtg agtgttccat tactcaatta gaaagtggaa tcgtggacta
    30121 tgaccaagta tttcagttta ttcaagattt gattcaagaa aatgatttgg aatgtatggg
    30181 gatttgttat gacccgtata acgctaattc gcttatcagt aaagctgaaa aagccaacta
    30241 cccaatgtta gaagtaagac aaggaacgat tactctaaac gttccgaccc gaacttttag
    30301 agaacaagtt tatgaaggca acgttattca caataaaaat acgattctca cccatgcagt
    30361 gaacaacgct attttaaaaa cggataacaa cggcattcag attaataaat caaagaacag
    30421 taacaaaatt gatccaatag ctgcattaat caatgcctat gtgtttgcaa tggattactt
    30481 caccacaacg gaaggagcga aagcagacaa tgaattttat acaagtgaag aattttcttt
    30541 ctaattacat tcataccgtt cttttacttc ttggattggt gtgtgtgttg gttgctatca
    30601 ccttactaac aaatgtctac tatggcttgt tagcgctggg catagtgctt attggggtag
    30661 cggtcatgct aaatacagaa cagaagggag gttaaaagat ggcatttttt aaagcgagac
    30721 aaaatacaac gggagatcct ttcctacaac atgtggtatc aatccaatcg gatgattaca
    30781 ccaccagctt tacaagcgtt cgtgcattaa gaaatagtga tgtgtttgca gccgttcgaa
    30841 ttattgctag tgatattgct tcaagtccga ttcaattggt caaaaacaat atgccacaag
    30901 ctgataatga actggtgaag ttactaaacg agaaacctaa ttcagaaatg gatggttggc
    30961 atttaaagtt tgctttggcg gtcaatatgt tgctaaacgg taatagcttt gcagagatta
    31021 aacgcaacgg tgaaaaggta gaagaaattc acttattacc taactcaagt gtaacggtta
    31081 ctcaattaga taatggtacg ttgtcttatc agattggtga taaaaaaaga cgtgtgaagt
    31141 ctagcgatat tttgcacttc aaatatttca cccaagatgg tttaacagga ttaccaccac
    31201 tttatgcgtt acgtgatgaa ctgaaaattc aacaagctgg caaccgcaca ttgcacaatt
    31261 tctttactcg tggtgtcagt gggtcaggta ttttgaaggt tcataagtca gatttagacg
    31321 gatcagcaaa gaacgcaatt cgtgaaaagt ttgaagaagc caacggttca agtagtggag
    31381 ataatgctct tagaacgatc attcttgatg aaacaatgga ctacaaaaca ttagaaataa
    31441 acaccgatgt tttgaagctt atcaattcca atgattggaa cacaaaacaa attgccaagg
    31501 cgtttggtgt accgattgag cgtttaggcg ttgaaaatga acattctagc accgttcaaa
    31561 gtaatctcca gtatatccaa agtacgctga ttcattactt taatgtgttt gtgagtgaat
    31621 tagatacgaa acttgaaacc aatatccgct ttaattccga tcagttacta gagactgatc
    31681 ctgaaacaaa agtaaaaaat gtattggatc aggtcaaagg gtcacttctc acgattaatg
    31741 aggggcggtc gaaaatgggg ctacccccca tggatggtgg cgatcgttta ctagcaagtt
    31801 tgaactttac gtatttagat acgttggaga aatatcaatt aaaagaacag gaaggagtta
    31861 caccagttga ataatgaaga agaaaaggaa aaacggctga cagaagaagc tgagctaaca
    31921 gccgattctc ccaaagtaga gaaagaaaat gaaaaacaac caacagacgg caaaactatt
    31981 tcaggctatg cgttgaaatt cgggcaaccg tcaaaagatt taggcggctt tgtagaagtg
    32041 attacacccg aagcattaaa agaggtggat ttatcaaatg tgttcttatt atatggacac
    32101 gattattcaa agccactagc tagtgtcaaa gcaggtacgt tgaaattgaa tgtcgatgat
    32161 acaggattac attttgaagc agagctaacc gacacgacct attcaaatga tgtgtatgag
    32221 aatatctcaa aaggggttgt tgattctatg tcctttgggt tcgtgcttgg attagattca
    32281 ttcgacaaaa aagaagatgg cacaattgaa cgatcaatag ataaaatcaa agcacttaat
    32341 gaaattagtg tcgtgaccgt tcccgcttat gattcatcaa atgtccaagt aaataagcgt
    32401 tcttacgaat cgtttatgag taacaaccaa gcaaaacaaa caaacaatag cttagaatcc
    32461 acttctaaag cacaaaagga gagtaataac atggaaaaaa ctttaatcga taacgaaaaa
    32521 acagaattgc gtgggtatga agaatatatc cgttcacaag gcgaagtgcg tgatggagtc
    32581 actactgtaa atgcagcggc agttgttccc gaagaagtaa tcggtgaagt ctttgatttg
    32641 aaacgttcaa attataactt agctcaatat gcaacagtaa aaacagtatc aaatggacaa
    32701 ggtaaatatc cagtagcaac taaccaacaa gcagtgttag caacaaaagc tgaacttgct
    32761 gaaattggtg atattgacgc tgaaatgttt acttcagttg attataaagt agaaactcgt
    32821 gctggtaaga ttgccttatc aaatgaggtt gtggaagatt cagcagtgaa tattgtacaa
    32881 gaggtcaaag atcagttagc gaaattggta gaaaacactg acaataagca tatcatggat
    32941 ttattaaaaa cattcactaa gaaaacggct gctacgttgg acgatttgaa acaactatac
    33001 aatgtggcat tagacccagc attaaataaa atggtaattc taaaccaaag cggatattac
    33061 cacctagata cattgaaaga ttcagatgga cgttacattt tacaacccga tgtgacagca
    33121 cctagtggta aatcattatt cggtatgcca gtagtattga ttgcagatac attgtttgcc
    33181 aatcctaaag ctggaacatt ccctatgatt atgggggata ttgcacaatc tatctttgtt
    33241 gctcgtagaa atcaagtaac gactcaatgg gaaaaattcg attactactc acaaggactt
    33301 gcagtgatcg ttcgcaacga ttataagaaa attgatcaaa atgcttcagt gtatattgag
    33361 tttacgccag ttgtaacacc aaaagtatag aaaaatattg ggcggcggtt tatcccactg
    33421 cctttttttc attaggagag ataaaatggt aattttggat agcataaaaa aaagtatgcg
    33481 gatcgatcac actattgatg atgactttat tcaacaattg attgatacag caggcgaata
    33541 tataaaaagt gctattgata gtagcgcaac ggataaagat atggataatt atcagcaatt
    33601 tgatttggcg gtgtcattac ttactcaaca ttggtatttg aaccgtcaag aagccagcag
    33661 cgaacggata ccagtaacgg tacaagcatt agtacaacaa atgagaggtg cttattatgc
    33721 caatcattaa gaatgtaaat gaattgactg agagagtcaa gtttaaaaag acaaaacggg
    33781 taaaagatga agatgggcag atggtggata gtgaagaaac tgtatttgaa tgttgggcta
    33841 atgtgcgttc acaaatgcta aaagacgtgc ttgctagtgt aggtaccatt cttgaaggaa
    33901 cgttgacgtt tattattcga tacgatcaag attatgaact aacaaacgat atgaaagtag
    33961 cttggaaaaa caagagttat aaaattattt caatcaatga aggaacggcg tttaaagatt
    34021 atacaacgat catagctaaa cagatttctt agaatgatta cacttgtaaa cgttgtgggt
    34081 atttagtata attaaggtag taaatgaagg gttagctgcc tgaattttgc aagacttagc
    34141 tagtcgaaat ctattgttgg actgaaaatt gtaggtgtgg ttgcaaacat gctggactaa
    34201 gaaagatgat agatgatgaa cgttcaattt taatacaggc atgctaggac tgattggatg
    34261 gttttggcaa gctgattgtg tagaaatcaa agaacacgtc tgttgtggac gtgttttttt
    34321 tgttataatt caaaagaggt gatttaatgg acaaagcaga gtttacaaat ttttattgga
    34381 agtattatct acatttagaa aatgagttta ttaataccac agattttgtg atgctcgatg
    34441 agaataattt caaaacattc tctattgaat atcaaaaact actactagcg attggttcgg
    34501 agtgtgagat tattttcaaa gaactttgcg gatttgatag cagtagcaat aaaagcataa
    34561 ctgattttaa agctgttatt aaatctagcg aattaatgtg tttggataat ggtgttaggg
    34621 ttatgaattc tttaacattg acttcactaa aaccttttgg agatgggtgg cctgaagata
    34681 caccagattg gtggaaaata tataacagag taaaacatgg tcgttcttca aattataagg
    34741 aagcaaattt ggaaaatgtg ttgtatgctt tagcaagtct atatttatta gaggaatatt
    34801 tgcataagaa agtaatacta gaaggtgaag tagactttat aagtccagag tcaaatctat
    34861 ttacgttatc ttgggaaaga aaacattcgt cgatgcaaaa agtgacgttt gaaaaagtgg
    34921 attcaaatta tgtagctcca ctcattgatt ttaaagaaca agaagaaatt taaaaacccc
    34981 cacggcttat tgaagttttt gtatgccata ttgtatgcca tttggtttaa atcgcttgaa
    35041 aaatagtgat agataactct aattcattta gtcataaacg ctgttttgat aacctttata
    35101 gttctttgat atgggtttct cattcatggc ggaagaagaa gaatagaaca aaagaatgaa
    35161 ttaataagat ataaaaaaag ggattgtgac aaacgtcttg tcacaatccc tttttccaaa
    35221 taaacgatgt tcaatcagct gcccctcggc attcgctcga aaaacggcga aatatgaaga
    35281 actattttgc catttgtttc tctaataatc aggtcaaaac gttgacttca cagtctcttc
    35341 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga
    35401 gaagctgttt tttgaaattg ttccttgtga ctcggagctg aacggcaggc tcacgacctc
    35461 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata agttcaacca aacgaaaatt
    35521 atgaggagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
    35581 cctcttttcg tttagcacta aatttcctta aaaagtagaa aagctttcga agcctcgttt
    35641 gtttctttta ctgtataata aaagaaaaga gtaacaatcg ctaaaagata taaacaagag
    35701 aaaggaaata caagatgatt ggtctgattt attttgtggt gatcgttctg gcgaacacag
    35761 ttggcgctat ttccgggatg ggtggaggcg tgttgatcaa accgattctt gatcttatcg
    35821 gcgcccattc tgttgcaggg atttcttttt actcaacagt tgcggtattc acgatgtcca
    35881 ttgtttcgac tgtgcgacaa gtatcaagcg ggaaatcact gaattggcag attgtcggtt
    35941 gggtatctag tggagctgtt gttggaggag tagtcggcaa cattgtattc gaagtttttt
    36001 tacagttatt tgaaaacgag aagcatgtgc agctgatcca aatttttttg acggtattga
    36061 ccttggtttt tgcgttcttc tacaccaagc atcatcagcc aaaatttcat ttgacgtcat
    36121 ggacttggta ccttatatgt gggggggttc tgggcttttt agctagtttt ttaggtattg
    36181 gtggtggacc agtcaatgtt tctttgttga tgctgatgtt tgcattgcca ataaaagaag
    36241 caacactata ttctctatct acgattttct tttctcaact ggcaaaattg gtaacgattg
    36301 ctttaacaag tagctttatg cgctttgatt tatctatgtt gttttatgtt atccccgctg
    36361 ctgttattgg aggtttgtgg ggagcaagat ttagcagggt tttgtctcca aaaaaagtga
    36421 cctttatttt tcaagtaatt gtcctcgtgg ttcttttgat caatctttac aatgctttcg
    36481 tcatcttaca aacattagga gaatctagac taacgcaagt caaaatcact gaaacatatt
    36541 gttctaaatg aaaaaaggag tatactgtga taaaaaaatt catttaaaaa aataaaaatt
    36601 ggctggtgat ttggatgttg cgggaaatgc gtttaggaga tgcagaagca ctacgagaaa
    36661 tcaatgcgga acaattagga tatgatgtac cattaaccgt tacggtgaga caaatgaaga
    36721 aactgttgaa ggagccagaa aaaaattttt tcttggtcta tgaagatcct acttctaaaa
    36781 aagcagctgg ctatgtacaa gcggagtcct atgaatcgat tttcagcgat ccgatgttca
    36841 atattatggc acttgctgtg tctaaacatg ctgaaaaaag aggaatcgga aaggccttga
    36901 tgactggcgt agaagaagcg aaaagacgag gaatatcagc aataagactt aattctgcag
    36961 aatacagagt agaagcacac cgattttatg aacgcatcgg ttatcattcc gataaaatgc
    37021 aaaaaagatt tttgaaaatc ctgtaaattt ttatcaaaaa atacctggtc atcaagccga
    37081 tacttgatga tcaggtattt tttttgacta agaaagtaat tgcatcaaga aacctgagat
    37141 gatcaaaacg atggcaccac caagccgatt gcccatttga gcaaaagcaa taagttccat
    37201 ccgattagct gcagataaga cagctacatt tcctgtacca cccatcgagt tgttgcataa
    37261 gccagcagta atagttgatt cgattggata taaaccaaac aaacgaccga caaatcctga
    37321 aactactgaa atcgcgataa cacttgtcaa acataagaca acaaattgcc atgtcaaagc
    37381 agaagccaat acatttagat tcaataacgc aataccgata ccggcaagaa cagctgctgt
    37441 caagtttttg acaatcaagt tgttgaacat gatagctgca tcttcgtaat attctggtaa
    37501 taggtttgcg attttacaga taacaacgat aatgatcatg aatgcgtaag catggacttt
    37561 aggaacaaaa tagttacaga tttgtcctaa gatgaagaaa gtcaatgata ccatcaaacc
    37621 gacacctaat tgtggaatgg aaggttttac atttctaggt tttccatcgc caaggtcgcc
    37681 tttttccatt aatttaccat taccgttgaa ttttggcatt gattgtcccg cacgagcaac
    37741 taacgctgca ccaatgatag ctaggacatt tgtcattgct gtcgcaggaa tcaggcggga
    37801 aatgatagaa gaagcgtccg tacctaaaac atttgcataa atcgttgata aaggtacagc
    37861 tccggctcca acaccaccag ccattgcggg caatgagata tataaaacgg aatttgcaaa
    37921 accatttccg ataagcatac ctacgagtcc tacggcaaaa aaggagacga ccattgaaat
    37981 aagtgcaacg ggaataaaac gaacagaagc tttcatcaat agattacggt tcataccaag
    38041 gatgctacca gtaatcaagg cagcaatata aaagtctaaa aagcccatat tgctgacaaa
    38101 gttttctgtg gattttacta catactcagg taagatccca aatgtagcaa tcgcggcaga
    38161 ggcaaagatc gcaaaaacgg agccaccacc taaataactt ttgacgatag ggatttttgt
    38221 tccgataaaa tgaaataaat taccgaaaat gaccaaaaca gcgattgggc caatcatatt
    38281 cgtcggtaat tttcctaaag ccattgcaat gatcaggaca aggaccatga tcaaataaag
    38341 gggcaaactt acgccggtaa tttttgttga ccaaaaccct tcttttttct tttcctcaac
    38401 tgcagttgcg gttgttgttt tttgtgccat ttttacagat cctttcttat tctgtatcgt
    38461 atgaaaaaag ccaaagagaa gcggcaaaag agcagtagtt ggctggctgc tgttttgccg
    38521 cagtcgcttt tttcttttta gacgtcatac aaagtttctg tatgttcttt tactataaca
    38581 atcttaatct tctaaagatt tatattcagg aacccattta gcagattcaa cagcttcttt
    38641 gatatccgtg atttcttcac gattcaattt ttgtgcaaca actgattttg cgacagtttc
    38701 agcaatcgtt tgagagaatt cagtgatttt tgctactgga ggcaagatag ctgctcctgg
    38761 ttttgttaca tcaacgattc cgcctaatgc tcgactagct tgagaaatga tttcagcatt
    38821 tactcgtgta gctgttgaag caatcaaacc aagccctaaa cctgggtaca tcaacgcatt
    38881 atttgcttga ccgatttggt aagttacacc gttatattcc acatcagcag cagggatacc
    38941 tgttccaact aaagcttttc cgtccgtcca ttcaatcaaa tcttttgctt ttgcttctgc
    39001 caattttgtt ggattagaca aaggcatgat gattggacgt ggggtatgag ccgccatttc
    39061 tttcacgatt tcttcagtga atgctccagg ttgggtagaa gtaccgatca tgatcgttgg
    39121 atggattgct tttacaactg cttctaagtt tgtcaattca tcagcattag tgaattctga
    39181 gcgtttacga gcgaatggga tttgaccagg agtcaatcct tttgtatctt caaacaataa
    39241 accctgttta tctacttgat agaaatgttg gcgtgcttct tcttcaggta caccttgacg
    39301 gatcatttca tctaaaagga tgtttgcgat acctacacca gcagtaccag caccaaatgt
    39361 caagatcgtt tgatctttca attgctcacc tgatatattc aatccaccta atacaccagc
    39421 tagtacaacg attcctgtgc cttggatatc atcattgaaa gtagtgattt tatcttcgta
    39481 tttttctaag atcgttgcag catttcctcg tccaaaatct tcccaatgta ataacaattc
    39541 tgggaataat tcagtagctg attgaacgaa ttgatcaatg aattcgtagt aagtttcgcc
    39601 ttctacacga gcatgacgat tccctaagta taatggatta ttcaataatt cttcgttatt
    39661 agtaccagcg tcgattgata caggcaagac ttgtgcagga ttgataccag ccgcagcagt
    39721 gtagaccatc aatttaccga tagcgatgtc aacaccgttc acaccccagt cacccatacc
    39781 taagattcct tctgcatctg tcacaacgat caaacgaatg tcgcgtccat ctgctgcgtt
    39841 tttcaaactt gctttgatat tttctggttc gtcgattgaa agaaatgcag catcttgtgg
    39901 tttcaagaaa atttcgttgt attgttcgat tgaatccgca acgacaggat cataaacgac
    39961 aggcatgaat tcaactaaat gttcgcccat caatttataa aacaatgtcc ggttcgtatt
    40021 gaatagattc attaagaaga tacgtttttc caaatcggtc tgtttgctta agtattgacc
    40081 gtaagcttgg acagattgtt gttccaatgt ttggacagta cttggaagca taccagtgat
    40141 tccgtatttt gcccgttctt cttttgtaaa agctgttcct ttatttagaa aaggattatt
    40201 taataagttt agccctgtaa gcatgaatga ttcctcctaa gttttaatac aaacgaagta
    40261 taggagtcag atgaggatat agtcaaaaag agcaggtata ataaatttta tttatatgag
    40321 taatagagtc aaaagaaatc aaaatagcgg tatttgttct ttttatatca acaatccttg
    40381 cttgattact atgcgtatct cttatatgct aaagaaagaa aagtagaaga ataaaggata
    40441 tgttagatag ataaatagaa tctctgaaga ataaatcacg tgatcgtgac gataaaatac
    40501 acttacgtca ggtgataaaa aatacataag tagaaaacta agaggaggaa cagaaaatga
    40561 aagaagttca attgatcaga aaaagcgaac tatcagaagg tggttgcaat gcatgcggcg
    40621 tagtagaagc aacaagctat acattaaagc ttggtgccaa caaagcgata atctctgagt
    40681 tgacagtagg cggtcttgtg gacagtctgg cgcttgctga aggttttatt ggagaagata
    40741 tttatgagat gttttcagaa atccgtcagt taaaaaaagg ggaaaactgc atagaggttc
    40801 atcatgagag cccgaatgtg agattcaaac gtggtgataa tgagatgatc ttcaataatc
    40861 atgtgtccga tcacaccgag ctatatggaa tcgtgaatca aatactaact gagctttttg
    40921 gattgggacc ttatgcgttc aaggaagaaa acggtaatcc aaaattaaac gaagaatggc
    40981 aagaaacgat tgaaatacaa agaaacaacc cgcacttatt tcaataggca tccacagtat
    41041 ttcgtaaaag ggactttgac acgacttttg tcaaagtccc atcgtttttt atctatttgt
    41101 gttctaaaca tttctttcag agtagtaaca actggaatga ctgaaatgta aacagattat
    41161 ttgcctgtat caatacggaa gagtaatata aagtgtgtaa acgctatcat attattcatt
    41221 caatttttta tagagggaag gcattgaaat ggaaaaaacg tatcaagcag gtacaaatga
    41281 aggaatagtg gattttatca atatggaaga tttagaaata gccgcttcac aagtgattcc
    41341 agctggtggt tatggttaca tcagcagtgg tgctggcgac ttatttactt atcaagaaaa
    41401 tgaacgggca ttcaatcatc agctgattat tcctcatgta ttaaaagatg tagaattacc
    41461 cgatacaact acacattttg acaaagaaac gctgactgca ccaatcatca tggcaccagt
    41521 tgctgctcat ggcttagctc atgtgaaggc ggagaaagct tctgcaaaag gtgtagcaga
    41581 ttttgggacg atctatacag caagttctta cgcttcttgt acattagaag agataagaga
    41641 agcaggcggg gagaaagcac cgcaatggtt tcaattttat atgagcaaag atgatgggat
    41701 caatttggat attcttgaag tggccaagcg taatggagca aaagcaatcg tattgacagc
    41761 ggatgcaaca gttggcggaa accgtgaaac agaccgcaga aatgggttta ccttcccact
    41821 gccaatgccg atcgttcagg cttatcaatc aggagtaggc caaacgatgg atgctgtgta
    41881 caaatcttcc aaacagaagc ttagtccaaa agatgtagaa tttattgctg ctcattctga
    41941 tttgccagtc tatgtgaaag gtgtccagtc agaagaagat gtctatcgtt cattagaatc
    42001 tggtgctggc ggaatctggg tatctaatca tggtggccgt caactagatg gtggtccagc
    42061 agcgtttgat tcgttgcaat atgtggcaga agcagtagat aaacgtgtac caatcgtgtt
    42121 tgacagcggt gtacgacgcg gacagcatgt tttcaaagca atcgcatcag gtgcagatct
    42181 tgttgctatt ggcagacccg ttatttatgg attgtcttta ggaggaagta caggagtcca
    42241 tcaagtgttt gatttcttta aaacagaatt ggaaatggtc atgcaattag ctggaacaca
    42301 aacagtagaa gatatcaaaa aaataaaatt gcgtgagaat cgttttattt aagaaacaaa
    42361 tgacgaaaaa aataggagtg cggcagatga tgccgtactc ctatttttat agttcaagga
    42421 tgtcactaat agaataattt gaaacgaaaa aataaaattg aaatattttt tcatacttaa
    42481 taaaaaagga agaatattca tgtaaacgat tttattgttt ttttctcttt tatacaaaaa
    42541 gggtcataat ttcaagtaag cggatgaatt catgaggatt ttttgcatac tgtctctact
    42601 atacttaggt cactaagaaa aaggagtgat ttattatgaa aaagaacatg attgggacat
    42661 tattactttc aacacttctt ctaggcggct tggcaacacc agcttttgct gagggtcaag
    42721 cgacttcaaa aggagatatt acctttactg aaccaacaaa taccgtggag cctcttaacc
    42781 ctactgatcc ttcaaaacca gtagagccag cagatccgga aaatccagca actggccaga
    42841 caggtccttt aacactggat gtagttcccg aattgccatt tggtacacac gagattgaaa
    42901 gcggaacaaa aacgtatcaa gtagacgctt caaaaaacga tacaccttat ctccaagtat
    42961 cagatcgtcg aggtgtaggt gcagatggcc aagcacaagg gtggaacgtg acagtttcgg
    43021 tttcagattt tgtaaacggt tcacaagttt tgcaaggcgc tgaattagat tttggaacat
    43081 ccactgttaa aagtacgtca gataacgaat ccactggacc aactagtcaa actgtaactg
    43141 ggttgagtaa agcaagtgca gcgacaccaa tatttacagc agcaaaagat caaggtctgg
    43201 gaacatggct ttctgtttat gatccagcaa atatcacact gaaagtgccg aaagcagcag
    43261 ctggaacatt cacggctgat ttgacttgga acctcgtggc tggcccagta gcatagagta
    43321 tgaaataaat tcaacaaact gcggtgaaaa tactataggt gaaggatttt ttaccgcagt
    43381 ttttgttgaa aggatgtggt ggacaatgaa tcaaagtaaa cgactgtttg tttcgttttt
    43441 ttctatttta agtctatttt tcatttttcc gtcaatcagc caagcaaagg attcagcagg
    43501 agactttgga atcaaaccgg tatttccaga gaaccaaata gataaagcaa tcggttattt
    43561 tgatttatta gtggcaccgg aacaaaatca gacactagaa gtaattatca gcaattcttc
    43621 agatgaagaa cgaacatttg aggtctctgt caatccggcg gtaacgagtg atggtggaac
    43681 gatcgattat agtcaaaaaa acccaacgtt agatgagaca ttgccgtttg atgtccgtga
    43741 cgttttgctc attgcgaaaa aggaaatcaa tgtatcagca catgcagaaa caactgtacc
    43801 tatagaagtg aaaatcccag caaaaagctt taagggacgt gtattagcgg gtattcatgt
    43861 ttcacctaaa gaagaagctg aaacagaaaa tgctacagaa ggagcacaaa tcaaaaatcg
    43921 tattgcttat aatctcgcag tcgttttaca agaaagtcaa gagacgatag aaccggatct
    43981 gaaactttta tcaggagatt tggacgaggt caacgcaaag ccaactgttc agcttcgttt
    44041 ccaaaatcct cagcctagga ttatcagcaa tttgatattt acgagcaaga tcttttatga
    44101 aaatcagctt tatattgaaa atacgtctaa tgcattttta gttgcaccaa attcgaattt
    44161 tcacttgaat cttgatttag ctggtgataa agcaaaagcc ggcgattacc gcgcagaaat
    44221 catagcaaaa tcaggcgaca gtaatgaatg gcgatttaca caaaacttta cgatcaaaaa
    44281 agaaaaagca caaaaagtaa atgaaaattc cgtttttgca gtacaagaac aatctttccc
    44341 atggctttat gcaggattgg ttgctgcagt tctagtgtta tggttgattt tgctgttttt
    44401 gtgggtccac aagaaaaaga aaaaaggaga aacaacatga tgaggtgatt tcgttgaaaa
    44461 catcgatctt attgggaggg attattgcaa gtttgctgtt cttcagctgg caagagccag
    44521 tccaagcaga taccaaaagt tcaggtacga ttacatttta tgatctaagt ccaattccgc
    44581 atccaagtgg ttcagtcaat ggcgcatcaa aacagcagac acagctaccg atttataaag
    44641 aatctaatca acatggcgta ttcccggaga cgaatgaaca aaagatccgt tttctttatt
    44701 tgatcattgg tatagaatta tggcttcttg cgtggttgtt gttatggaga agaaaggaaa
    44761 aggaggatga acatgttgct acaaaataaa ttttcatttt ttcgctcctt cttttttact
    44821 tcgctttttg ttttaagtat atttatcttt tcaccttcgg cagaagctgt tggagcaacg
    44881 cctcctgcca tttcttatga tccatacacg tatgaaaata tcgtacctcc aaatggtgga
    44941 cgtagtgcag tcaatggcgg gaagcttgtc caactaactg gggctaacca attgcctaaa
    45001 ttgactgcta cttatttgaa cgatggttca aatgatcctt ggaaaggtgg cacggtccgt
    45061 tttcattggg aaagccatgt gaaactaaca aatgtacttg atttggtgaa tatcactgca
    45121 caaggatttg catttgtggc gatcatcaat ttgccggaac atgtatcaag cagtgatgta
    45181 ttaaaagcaa ttgactggaa caacgcctac ttgtatattg ccggtgcccc catcaaggtt
    45241 actagcggaa gtttattatc agcaggaagc cataccttgc gttttgggat ggggtcgtgg
    45301 gatacaaata ctctattgac agatattatt gctattatta cgcgaggcga attcaacgtg
    45361 gacgatatcc cgcttcaatt tgatgttaca gctaatgtcg cgaagatgac agaaaacgga
    45421 gataaatatg atacgagtcc aaacaaaatt ttgaccaagg gtaaattaca accttctatg
    45481 tctaaaaagg ccgttttttc tgttgatttt tatgattcga ataatattat ccaagatcgt
    45541 atgttcaatt cgattttagc tccagctgca ggtagatttt atcctaaatt aactggcgga
    45601 aacttgaatc caggagcata tgcattttct gcaagtgcag agataaatac gtggaatagt
    45661 tacatctcac caagtgattc aacaggaaaa tataatacat ttgctgatac cacagaatac
    45721 gtggatggaa ataaccagaa ccttccaggc tctaccaata gtcggacatt gacgatgggg
    45781 ctgactgaag gacgttttga tcagttgcca tttagtcgct ttgatcgcgt agtcaactat
    45841 tttacaggaa aaaacgttac acaaggagca atactgactc atactccagc aagcacaagt
    45901 ggattgaatc agacagccaa tatcgtgtac agtgggaaag atgcgcaagg cggattgctt
    45961 tctcctgttg ctttaaaggt attggagcag tacaagctag acggaacggt tttaccaaca
    46021 aacttaacag ccgatagtca gtggggaaag ctcctttctt ctgcatcgta ccaagtcacg
    46081 gccaattggt cagcaactga actaaaaaag ggttcgatcc attacaggct ttattcgaaa
    46141 ggaacccaaa aattagcgac aggatggaca gatcaacttt tccaaacgat ccaatcaaat
    46201 aacgggggtg taaatacagc tacttctaca ctacctgcat tggcttcagg gcaatattat
    46261 tttgattatc gcttggtgga cgatgtactt actaacatgt atccgtcttt tgcgtacaaa
    46321 tggcagtctg aacaagctgg aatcaccact cttcctcaaa taacagtggc taattttcca
    46381 tctgtgagtg cagatagtca gctaaaaaac cttagcagac aacctgagac aggagatccc
    46441 ctgtctgctc tatcaggaga tacgattcaa gaaaatgtga cattcacgtt aacaaaatct
    46501 ggggatgcga taaccgacaa aaaaatcacg atctcacttc ctgaaaatac tacttatatc
    46561 aatggttcct taaaattaaa tggtacaact atcgcagata ccggcattca acaaggagtt
    46621 tctgtcccag cagatctatt gagcaaaata ggagatacca ttcatcttac ttacaactat
    46681 caattaaata ctgtagatac ttctttacaa tccgtcagca tactgacaaa ggcagctgta
    46741 ctttccagca atattacatt agcagatggt gctaagcttc ctaacccagt tgtccaaaca
    46801 tctgctaaaa caattcttgt ccccaaacaa gaattaacat tagttaatgt accagatgat
    46861 tttacatttg gtaacgatct ccccaaaccg ttgaagacaa gttattatga atcaaaaggc
    46921 gatttttctt ttgatgtgcg ggatactcgg cttccaagca cgtctccttg gcaactgaca
    46981 ggtacattga cttctttatt taagaatgat caaggtcaag aattaagcgg aacaaaatta
    47041 tacttcaatc attctggatc aaagcaattg attcagcaag gtcaaaacac gttgatttat
    47101 gagagtgatg gaacagctaa aggagaggtt ctcgttgact ttcccgatac cgatggttta
    47161 ctcttagagg taaatagcag tacgaatgct caacctggcg caacgtatca aggcatggtc
    47221 acatgggaac tgacagctgg gccaaccagc taagtgatta gaatttgtat ggatctgtga
    47281 taactgtcac agatcctctt tttcaaggat gagaaggaaa cttctgctgt taaaatcaga
    47341 aatgcttgaa ggagtggact tggaactttt cagtttgctt gaaaaaaatg aacaacggca
    47401 aatcaaactt ttccaacttt tattagagga aaaaggagag gtttccgtta ataacgtact
    47461 tacccgctta tcagttgatc gggctacctt gaaagaagat ctgttttatt tacaagctaa
    47521 cttggaaaat atcaaacaat tggaaattgt tatccaagaa gggaatattt tattaatcag
    47581 acatggaaat atagcagcag tcgatgttta ttatcattgt tacttgcgct attctacaaa
    47641 atatcagata cttctttatc tgttggaaaa tggctcattt gatcggcaga aacttgtcat
    47701 ggaacttaat atcagttcgg ctactttgtc tcgcagagtg aaggaattaa atgattcttt
    47761 aaatgagttt catctgaaaa taaaaagcgg gcggctgacc ggtccagaaa gtcaaattcg
    47821 ttatttttat ttccaattat tttggtttgg acgtccctat cttgaaaaca aaaaagaata
    47881 cagtgattta tcaggaaaca cttttttaga tctgctgcaa aaagagttta gttttgctat
    47941 tacagaagaa ggacgattga aatttgaaat atggctttcc attatgaaga agcgaatgcg
    48001 ctgtacgaaa aaatccaaac cagattatag caatcttcct gttggattgt ttcaaacgac
    48061 acaattttta ttggacctcc atacattttt gacccgttac ttctttcaat atgcctttcc
    48121 ttggaatcaa aatgaaacgt tggtttttta tcttttcatg atcagtaact ttacattaga
    48181 tacacataac tcgcatgtcc aggagttttt gataagcgaa aaatacaaag acgacgtagt
    48241 agctcaattg aatcgaacgt ttaaacaatc gatagaagaa tatttctctt tttatgtttt
    48301 tccagaagag atttcgcaaa agattgcgtt gacagtgatg caaaatcacg ctcattttgc
    48361 ctattttaga ggatggatga atatttttgg ccaagaagga ttaaaacagc ggttaactga
    48421 agtagaagat aaacaattcc tggttttatg tgaaagattg gtcagacaaa catttgctat
    48481 tgttgatttt actgaagtag acgaggaaaa tgcccgcttt gaattatttg ctcgctatgc
    48541 atctatttta cggacgctga tcaagcagtt agaattacgt ctgcaaattg cttatgatct
    48601 tccttatgaa aggctaatgg cggatatcgt gaaagaaatc atccaggaac aattgctgaa
    48661 tacgatcaaa tatgatttag tcgaatacga gaaagaaaag cagtatgata tcattttgac
    48721 tagccaattg aaagagtacc cgtcacagaa gtccgctaaa gtttttgtat tcactagcaa
    48781 cgaatataat tatgatttcc cctatttgaa ccaattttta aaagaatgtt atttagaaaa
    48841 attgaattta aggatgaata aaacatgaaa atcgtgatca ttggtgcttc ccatgaaggc
    48901 atcacagctg ccctaaatct aagaaaactt caaccgaaag cagaagtttt actgatcgat
    48961 gaagatcata aagatggact aggctatgtt tcaaatggaa tcaaccttta tttaaaagga
    49021 aagatacgta gtttagctga agttgcccat aacatgcgga ctcttcaaga aagtggggca
    49081 aaactgatca ccgaatggag agtaactgaa cttgatccag acaaacatca gctcatcctc
    49141 gcatccaaag aaggaaaaaa cgagaccata acctatgata aattaatcgt agcgacaggt
    49201 tcctctcctg tcaccttgta taaacaaatt gaagctgaaa atgtctacac ctataaaaat
    49261 ctagtgcaat ctaagcaagt acttgccgct ttaaaagaag caaaagaagt agtgatcttc
    49321 ggtgctggtt acattggatt ggaattagca gatgctttga gaaataaagg atacattatt
    49381 catctagttg attatatgcc gaatgtttta tcaagatatt tcgataagga tatgatcaac
    49441 agttttcaaa atcagctgca aactaagcaa ataaattttt atccaaatga attcttgatc
    49501 gattggaaaa aatcagaaga gaaagtagta tccgtacaac ttctgtctca aacaataaag
    49561 gcagatatgg tcattttttc tgcccaaact cgtcctaata cgacattgtt aaaagaaaaa
    49621 gtggctttgt atgaggatga gactgtgata gtcaatgaat atctgcagac aagtgatccg
    49681 gacatctatg cgattggaga tatcgtccca gtcagttttg acaaaaataa acggcatctc
    49741 tttttacctt tagttactcg agctgtacat atggcaagag cagtagcgct aactttgagt
    49801 ggtcagccaa ccgcttacga cctcagacaa aagataacag cagcagtcat tatagactac
    49861 tttttaggaa ctgtagggct gacagaagat gaagcgccat tcttggaaca aaataccggt
    49921 tcgtgttcag gagaatttga tctatttcca caatacgacg aagaaaacaa aacagtaaat
    49981 gcaaagctga tctaccatcc ggatacatta gaaataattg gcggacagct gataagccaa
    50041 gaattcttat tatcagattt gaacctgctt gcggatatcg tgaaacacaa aactacgatt
    50101 ccacagctag cagttgagga ttttggattt ctgtctgaat acacctctcg ttttcactac
    50161 ctcaatgagt tagcttttaa agtactagct aagagagcaa atagcaatag agtagataaa
    50221 agactgtgag aaatatcacg gtcttttatt gtgtggggat ataataaata ttaatcaatc
    50281 gagaataaaa aattcatata atgaaatagc ggaagaattt tttgagcata atattcgcca
    50341 aaaatttatc aagtgataaa attgttgtat aaaatgatta tttcaggagg aggagttaaa
    50401 tgtttttagc attaaatgaa attatgcatt caaagttacg ttatgcgtta gttgcagggg
    50461 tgatgttttt gatcgcctac ctggtatttt ttttgactgg gttagcttat ggcttagctc
    50521 aagataatcg aacagcagtc gacaagtggg aggcagattc gattgtgctt tcgaaagatg
    50581 caaattcaaa tctagggatg tccatgatta ctaagaagat agcagaagaa gtcgaaggtg
    50641 ggaaagtggc gtatcttgcc caaacacctg gagtcgtgac tagtaaagac agtacagaag
    50701 aaggaaaaat caatgttagc ttcttcggta tcgataaaaa tcaattcatc atgcccaatc
    50761 ttgttgaagg aaaagctttt gataacgatg atgaggcagt aggcgatatt agtttgaaag
    50821 aagaatatgg tctagccata ggagatacag ttaagttatc tggtagtgac aagacgttta
    50881 aactaactgg ctttacggat catgccaaat tcaatgtctc accagttctc tatacaacca
    50941 tcaatgctta ccaagaaatc cgcttcgaaa aagaagatac aagtgaaaat gcacggatta
    51001 acgcaatagt tgtcagaggt aaaatcagta atttacctga agatttagaa cagatcaaaa
    51061 tttctaaatt catcaatgaa ttacctggat ataatgcgca agtattgact tttggattta
    51121 tgatcggatt tttgatcgtt attgcggcta ttgtgatcgg gattttcatt tatgtactga
    51181 cgatgcaaaa gattaatatc tttggtgtaa tgaaagcaca agggatcaca ggaggattta
    51241 ttgcgcgttc tgtagtggca caaacattca ttttatcatt tgttggtatt cttcttggtc
    51301 tattaggaac agtgggaaca tcccttgttc tacctgatgc ggttccattc caaagcaatt
    51361 ggctgttttt tggcgtcatt agcttgttga tgttagtagt agctgtgtta ggcgcactat
    51421 tttctgttcg gacaatcgta aaaatcgatc cgttgaaggc aattggataa gaaggaggaa
    51481 gaacatgaaa gcaatagaat ttaaagaagt agaaaaaagt ttccaagatg gcgaccaagt
    51541 agttaaagca ttgaaagaaa caaccatgag tatcgataaa ggggaatttg ttgcaatcat
    51601 cggtccttct ggttcaggta aaagtacttt tttgactttg gcaggcggat tgcagacgcc
    51661 tagcagtggc tctgtattta tcaatgatca agaattcagt aatcaaaaag aaagcgaacg
    51721 cgttaaacta cgttttaaag aaattggctt tattttgcaa gcttccaatc tggttccttt
    51781 tctgacagta aaaaagcagt tattattagt agataagatt aaacatgaac atcgtcaaaa
    51841 agaagcaaac gaattatttg agcagcttgg cgtggataag ttgatcaaca agtatccaga
    51901 agaactatct ggtggagaac ggcaaagggt cgcaatcgct cgtgcattgt acaatgatcc
    51961 gtccattatt ctagcagatg aaccaactgc tagtttggat tcagaaaagg cacgagaagt
    52021 agtcgatatc ttggcaaaag aatcaaaaga caaaaataaa gcaacgatca tggttactca
    52081 cgatacacgg ctgattgaca aatgcgatag aatatttatg atggaagatg gtgtattgaa
    52141 agaaaaagaa taaccaaaaa gcaggtggaa aaatggtcca tcgatacaac aaagatacac
    52201 agcaaaaaat tttgaaagaa gcgactgact tgtttatgtc caagggatat ctcggcactt
    52261 caacaagaga aatcgcacaa aaagttggga tcacccagcc caatctctat cattatttcg
    52321 gtgataaaga aaaactatat acggcagttt tagaaaacca cttaaaagat gttggaaaag
    52381 ctttgagaga aatcgttcaa acaagcgaaa cgggatttca agtaaccttg acaaaaatgg
    52441 cgcagttttt gattgagaca catctggtcg atttgtttct gatgcttcac gatcttgaaa
    52501 gcaatctttc aaaggaaaca agagatcatc ttttctttct atggaagaaa aattatcgtg
    52561 aaccttttga agaaatcttt tcagcaaacc aatctgtgct aagaaatggg atttcaaaag
    52621 aaatcgcagc aaggcatttt ttcttagtgt tagcacctta catcactcgg tcgaaaagac
    52681 ctgtggaaga accgctaaat gtggcgcaat tgatcgattt atatttgaat ggtgtattag
    52741 gagaagcgta aagctgttat atagggagaa aatagttagg tgaagaagca agatatgacg
    52801 aagatcgtta tatcttgctt ctttatatag gtagtttttc ataaatacaa atttgttatt
    52861 ctctcatgat tatcagggta tatttttatt tattatgtcg gtgttccagg aagtgtttta
    52921 atttaataac gaagaaatta attgtttgta gaatgaagga aactatttat tgggtagatg
    52981 aatcaccacg aatagtcaaa aggagatttc aggatatgcc aaaaatgaaa aaaatcattt
    53041 tagcagtaat gttattttct acggttatat cttttacagc cccttttttg cttacttaag
    53101 ctgattttaa gttttggccc ttagtctgga tctacgaggt gagagtcaag aatgaaaaaa
    53161 ataattggag cttttgtatt tgcaatcatt atcattatta tctgttttca aatagatgtt
    53221 tttggtcaaa aaaagatagt tggacaaacg gtcagcagta cgaacagtac ggttacaaag
    53281 acaaaggaat catttgatga tacagaacaa gagacattaa tcaaaatcgg aaaaaatgac
    53341 cgtacactat atacagatga ctcgctaact accccacttg caactataaa cggcggagag
    53401 ctggcggagt ttctttctga aacaaaaaat gcttatcaga tcaagacaaa tgatggctat
    53461 actggatatt tagccctcga agatggaacc aaagtcacaa agaatataca aacaaaaccg
    53521 gaagagctat ctgatgcagt gattgtatta gatcctgggc atggcggaaa tgatactggg
    53581 gcattaagca atgatgaaca aacagaagaa aaagatataa cattaagtac agccataaaa
    53641 gtaaaaaagg cgctagagga tgctggagca acggtttatt tgacccatac tacagatgaa
    53701 ttagtacagc ttggagatat atgcgactac agtgaagaaa aaaaggcaga tgtctttatc
    53761 agtttacacg cagactctac ggaatatgcg aatgaagcaa ctggaatcac gacatattat
    53821 tactatggac aagaggaaac attagcacag acaattgctg actccttcac tgatctgcct
    53881 ctggattcaa gaggaatctc cactgggaac tatcaagttc ttcgagaaaa tcttcaacct
    53941 tctattttaa tcgagatggg ttatatgaat aatgatagtg atttaaagga attagtaaca
    54001 gacagttatc agcagcagat tgctgcaagt ttgacacaag cattgaccac ttattttcag
    54061 taataaataa gcccagccaa acaaaaatag ctcctcatat ttttcgtttg gccgggctta
    54121 ttatcgcaga tgcaagcttt taaacaccgt ttaaggaaga ggtcgtgagc ctgccgttca
    54181 actctgagtc acaaggagca atttcaaaaa acagcttctc atgtttgatg actattgtga
    54241 cttgtgaccg aggagttggc atgagaacac cgtttaagaa aaaggttgtg aaatcaacgt
    54301 tttgttctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    54361 gagctaatac cgaggatcag atgattgaac accgtttatt cggaacaagg gactgtgaca
    54421 tgacttttgt cacagttcca ttattttttt agtagcttca aagctttttc tgtattggca
    54481 aaatgaagtt tggcatattt ttcaagcata ggcatgccgc ctttctccaa tagttttgcc
    54541 gctacttggt cagatttcgt tccagctcct gacggaaggg tgattccagc ttcagctgat
    54601 gctttgtcta actcttccaa aaagctggca cgactgatga tagaagcagc agcaacagct
    54661 aaatgatatt gttctccttt agtaacgaag tacagttttt cttgtaattg atttttttct
    54721 aaacgcgcat attttttaaa atttgcttcc ggcgtaaact gatcgatcaa aatcgcttct
    54781 ggtttttctg gctcgatttt ttttagcagc aaatggatcg cttgattgtg caaagcgact
    54841 ttcatccgaa cggcattgta ttttggttgg atctcattgt atttttctgg agataagaca
    54901 agcagtttat aggggatggt tttttttaag atgtcagcaa gctggatgat ttggctatct
    54961 tttaactctt ttgaatctct aacaccaagt ttacgtaatt ctgcaagctg atcacgtttc
    55021 acataagcag cacatactgt tagcggaccg aaatagctgc catttccgac ctcatcacta
    55081 cctaaaacgg ataattgact gatatttttt ggcaaagaag aggctgtttt tgctggcttg
    55141 gcttgtttat cctgtgcctc cccccattta tcagcttcta ttgctgcatt cgtaccttga
    55201 aacatcactt ttccggatgt ataagcggta atggtcgtcc cgtctttttt tgcagcaaag
    55261 ctagtataag gaatctgttt atttaacaaa taagagcgat agtatgcttt catttgacta
    55321 agttggtcgt ttgttgcttt gataatgacg ttgctcataa aattctcctc gtttttatac
    55381 ataaaactat accgcaactc acgacttttc gttttctaag agattgcgag acatagcatt
    55441 tttttaaagt ttatcatata atagttaagt atgtgaaaaa tatttgtgga gaacggtgaa
    55501 agaaccaact tcatggtaca ctaaataaaa ctttcatcaa ataggaggaa atgattcgta
    55561 tggcgcacga aaaaacacgc tacaaagcgg tgattgccaa ccaaacatat actattatcg
    55621 gacgggaaac caagcatcat atggatatcg taacgaagct gatcaatgaa cagttagcag
    55681 aattgaaaca attatcacca caaatggata atgaacaagc agcaatcttg atggctgtca
    55741 atgcgctttc ggatcaattg aagaaacaag aaagaatatt ggagctggaa gaagaaacag
    55801 cagaattaaa gaaaaaaatg atcaaattta cagagcttga aaatcgtgtg aaacgcattg
    55861 aagcaatcga gaacgaagct cgggaagtat tgaaagaaaa tggtcaagca gatcatacca
    55921 ttaaaaatca tgtggaagca caacagattt taaacgagaa acgaaaagga caaattaaac
    55981 aaaaatcttc taattaagat ccttttatca aaataagaaa aatttaaaaa agttagaaga
    56041 gaaaggaacg tagcatgctg agtttgctga ttttattttt attgctaatt gcgttttttt
    56101 ctggagcacg tcgcgggttt gctttgcaaa tcatattcgc gattggatat gtattgtcat
    56161 ttattgctgc ccagcatttt tacaaaccat tagcaagtca tttggaatta tacattccat
    56221 acccagctgt caccccaaca tcaaagttag cattttttga tcaggtattt gcctttcacc
    56281 tagatgaagc tttttatgca ggaactgctt ttctgattat tttgctaatt ggctggctgc
    56341 ttacccgatt tgtgggggtt tttgtccatg gattgacgta tgtgccgatc ttgagacagg
    56401 tagactggat tgcaggaggc atcctgtcac taatcatggc ttacgttact atatttttga
    56461 ttttgcagtt acttgccttt gttccattag atgtggtcca aaatcaattc ggcgagcata
    56521 gtttcgctcg ttttatcgtt gagagaacgc catttcttac caacaagatg tatgagcttt
    56581 gggtaacgaa tattttgaat taaggaaaat agtgctggaa aatatttcgt tttttgtaca
    56641 gagtagttta tttaagaagg ctgcgtagcg tatgtccggc acagctcttt tttcatagtg
    56701 aaagataagg tgataaaatg aataaccgaa tccttgaaac attggaattt gaaaaagtca
    56761 aacagatggt cagacaattt gtagtgactg cacaaggaaa agaagaactg gcagaattgg
    56821 taccagtgag tgagaaacaa acgatcacaa actggcttca agaaactgag gatggattga
    56881 aagtgcaacg tctgcgaggc gggatcccta tacctaaaat tgaaaatatc cgtccccata
    56941 tgaaacggat tgagatcgga gcagacttga atggagtgga gcttgcacaa gtttccagag
    57001 tattgtctac gacaagcgaa ttgaatcgtt tcattgatga tctttctgac agtgaaatcg
    57061 aatttgcacg tttgtatatg tgggcagatc agttagtcac gatccctgtt ttgagccgcc
    57121 gtttaaagga agcaatcgat gaagacggac gcgtgactga tgacgcttct cctgagttaa
    57181 agagcataag acagaacatt cggcgaagtg aacaagctgt tcgtgaacag ttagatggga
    57241 tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat catcaccatg cgaaatgacc
    57301 gctacgttat cccagtcaaa caagaatacc gcggtgtatt tggcggtgta gtccacgatc
    57361 aaagtgcttc aggtcagact ttatttattg aaccaaaaca agtggtggat ttgaataatc
    57421 gtttgcgtca ataccaaatc gctgaacgaa atgagattca acgtatttta agtgaacttt
    57481 ctgctgaact tgttccccat cgtcaagaaa tcatccacaa tacttatgtg attggtaaaa
    57541 tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt aaaagccatc gtgccgggaa
    57601 tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca tccactgatc gatcaggaaa
    57661 aagtagtttc taatgacatc acaatcggaa aagactatca agccatcgtc attacaggac
    57721 ctaacacagg tgggaaaacg atcacgttga agactttagg tctgttacaa ctgatggggc
    57781 aagcaggatt accgattcct gcaggtgaag aaagtcaaat cggtattttt gaggaagtat
    57841 ttgcggatat aggagacgaa cagtcgattg agcaaagtct atcgaccttt tcttctcata
    57901 tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa aagtctggta ttatttgacg
    57961 agctcggtgc aggaacagat ccgcaagaag gtgctgctct agcaatcgca atcttagatg
    58021 atcttggtaa aaagtctgct tatgtgatgg caacaacgca ttatcctgaa ttgaaagttt
    58081 atggatataa tcgggcaaat acgattaatg ccagcatgga gtttgatgtg gatacattaa
    58141 gtccaacgta tcgtttgctg attggcgttc ctggacgaag caatgcgttt gaaatctcta
    58201 gccgattagg tcttgataca gaagttattg atgaagcgaa gcagttgatg aatgatgaga
    58261 gccaagattt aaacgaaatg atcacagatt tagagaatcg ccggaaaatg gcagagaccg
    58321 aatatctgga aatgcgtcat tttgtttctg aggcacaaga acttcacgat gatttgaaag
    58381 aagcttacag ctatttcttt gaagaacgtg aaaaagaaat ggaaaaagca aagaaaaaag
    58441 caaatgaagt cgtgtcagag gcggaagaaa aagcagaaaa aatcattgct gatattcgta
    58501 aaatgcaaca gcagattggg caaggaaatg tgaaagagca tcagttgatc gatgcaaaga
    58561 cccaattagc aaatctacat caagaagaaa ctttgaagaa aaataaagta ttgaagaagg
    58621 cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt ggttacgact tacggccaaa
    58681 gagggacgct tttacgtaaa aacgggaatc agtggcaagt agaaatcggt atattaaaaa
    58741 tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca aaaagagccg acacaacgag
    58801 ttatccatgc tgtacgatct gaatctagta gtcacgtgcc gaatcaattg gaccttcgag
    58861 gaaaacgata cgaagaagcg ctaagtgagg tagatcaata tttggattct gccattctag
    58921 ctggttatcc tcaagtgact attgtccacg gaaaaggaac aggcgcactt cgcaagggaa
    58981 ttactgatta tcttaaaaac catcggagcg tcaaaagctt tgagtttgca ccggcaaacc
    59041 aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag cttactaaag gtaagcagat
    59101 ggttcgctat ccaagtatag aaatgctata ctagtcatag attttaaaaa tggaggtctt
    59161 tgatatgtca caagtaatta cagataagac atttaatgaa gaaacagata aaggacttgt
    59221 attgattgat ttttgggcaa cttggtgtgg tccatgtcgt atgcaagcac cgattttgga
    59281 tcagttggaa caagaatatg atgaagaaga attcagaatt gctaaaatgg atgtggatga
    59341 aaatccagaa actcctcaac aattcggtat catgagcatt ccaacattaa tgttgaaaaa
    59401 agatggtcaa gtagtagaaa aagctgttgg tgttcattca aaagaacaac tacgtcaaat
    59461 gatcgatcaa tatttataaa aacttacagc aaaaataagc tgagggtggg actgaatcca
    59521 gttccgtcct cggttttttt gcggttgagt taaggttatt caaattaata ttttttgagt
    59581 ataattacgt tgtcaggctg gattgttttt ttgacagaca tgctataata actttaattg
    59641 aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa caaattagct ttacttcctg
    59701 atcagcccgg ctgttacttg atgaaagata aaaatggaac gatcatttat gtcggcaaag
    59761 ccaaaatatt aaaaaatcgt gtccgctcat attttactgg tagccataat acgaaaacgg
    59821 aacggcttgt tagtgaaatc gaagactttg aatacatcgt tacagaatcg aatatcgaag
    59881 cattgctctt agagatcaat ctgataaaga aaaatgatcc taaatacaac attatgttaa
    59941 aggatgataa aacctatcct ttccttaaga taacgaatga aaaatatccc cgtttggtga
    60001 ttactcgtaa ggtactaaaa gacaaagcac tgtattttgg accatatccc gatgtcggtg
    60061 cggcgaatga gacaaagaag atcctcgatc gattatttcc tctgagaaaa tgcaaaccta
    60121 gtcagacgaa ggagccttgt ctttactatc atttgggaca atgcctgtgt ccttattatt
    60181 tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga agtgaaaaac tttttaaacg
    60241 gaggacacga aaaaatcgag tcagagatcc atcagaaaat ggaaaaagct gctgaaaaca
    60301 tggagtttga aaaagctgca gaataccgag accagatccg tgccattgag acaatcatga
    60361 ctcgccaaaa aatgacaaat actgatttac tggatcgaga tgtatttggt tatgcgattg
    60421 ataaaggatg gatgtgtgtc caagtgtttt tcgtaagaca aggaaagcta attgaacgag
    60481 atgtttcaat gtttcctttc tataacgaag cagaagaaga tttcttgact tacctcggtc
    60541 agttttatca ggaaaatgag catttcattc ctaaagaagt cttgattcct gatacgatcg
    60601 acaagcccag tgtagaagct ttgttagcta cgaaagtgct gcaaccaaaa cgaggagaaa
    60661 agaaaaaatt agttgaattg gcgagtaaga atgcctcagt tgctttgaat gaacggtttg
    60721 atctgattgc cagaaaacag gaacggacga tcggagcagt tgaacgttta ggaaacgcga
    60781 tgaatatccc gaccccaata cgtatcgagt cttttgataa ctctaatatc atggggacga
    60841 atcctgtggc tgcgatggtc gtattcattg atggaaaacc ggcaagaaaa gaatatcgga
    60901 aatataaaat aaaaacagtc aacggaccgg atgattatgc atcaatgaga gaagtcatct
    60961 atcgcagata ttcgcgagtg attcgcgaag ggttgccttt acctgatttg atcttgatcg
    61021 atggaggaaa aggacaagta gatgtggcaa aagatgtctt agaaaatcaa ttaggaatcg
    61081 atattcctat tgctggcatg gtaaaaaacg ataaacataa aacaaatgag ttgctattcg
    61141 gtacagattt ggctgtagta ccgcttgagc gaaattcacc agagtttttc ttactccagc
    61201 gaatccaaga tgaagtccat cgattcgcaa tcactttcca tcggcagcta cgcagtaaaa
    61261 acagttttgc ttcccgatta gatgaaattg aagggttagg accaaaacga aaaaaaatgc
    61321 tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc cacgacagaa gaattacaag
    61381 aaatcggact gccgaaaaat gtcgcacaaa acgtatttga taaattacat caggaatgag
    61441 gaaagggcac tttacaaaga aagtaaagtg tcctatcatt ttatgtacaa caaaaagagc
    61501 gtttacgttt tgttggtaaa taataaatcg cttacgtctg gtgtctaagg atatatgtga
    61561 tttcttgaag aaggagtgaa gaaatgttag aagtcaagga tcttgttaaa acttttggga
    61621 actacacagc tgtcgatcat gtctctttcc aaatccccga tggaaaaatc atggggctaa
    61681 ttggccaaaa cggggctggg aagacaacga cttttcgttt gatcttggat tttttgacac
    61741 aagaccaagg agaagtgtta tggaatggcc atcatcttgg ggagaaagat tatgatgtca
    61801 tcggttatct tccggaggaa cggggacttt atccgaaagt aacgattcaa gaccaactgc
    61861 tttattttgc agaacttaga gggaaatcaa gaaaagagat tgaaccaaaa atcgactttt
    61921 ggatggaaaa atttcaggta aaagggaaaa agacggataa agtaaaatct ttgtctaaag
    61981 gaaatcagca aaaagtccag ctgatcgcca cgttaatcca tgaacctaaa ttgatcattt
    62041 tggatgagcc attcagcggg ttagatccgg taaatgctga attgctaaaa gatggaatat
    62101 tggaattgaa aaaacatggt tcatgtgtca ttttttctag ccacaatatg gataatgtag
    62161 aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa aatggttttg aatggaaaag
    62221 tacatgagat tcgtgaatca tttggccgta cgaagttatt cttggaatct ggtttaagcc
    62281 aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat ccttcatgaa gacaatacgt
    62341 tagagattac cttagaagat cctgaagctg ggaaagaaat ctttactcgt gcgactcagt
    62401 tcggctacat tcctatgttt aaccagcaac cgccaacttt ggaagagatt ttcaaattga
    62461 aagcaggtga aatcgatgcg taagttttgg attattacca aagatgtcta tttaaaaaat
    62521 gttaagtcga tttctttctt gatcatgata ttagtccctt ttatcatgct gggtatcatt
    62581 tatgtagcag gaaactttgc tcaacaaaat agtgatgtca ataaaatcgg cgtattggct
    62641 gataatgagc aaatcagtca gcaaatgagc caagtcaaaa cagatgattt tgagttcaaa
    62701 gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg aagaaaaaat cgatgcgtat
    62761 ttagtagtct caactgagaa tgataagata aatgggatgc tttatagcga aaattcatta
    62821 ggacaaagta cacaactact gatacaacaa caactgactg gcttgcaatc gatgcttcga
    62881 gcgagtcaat taggcatttc accagaagaa gtagctagct tgagccaaca agcaagtttg
    62941 actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga cagtcggaga agataactcg
    63001 acagtccaat acgtcgtaag ttatgtagca acgattgtct tgttcattat tattttgacg
    63061 tatgcgcaga tcattgcaca agagatcgct tctgaaaaag gcacgcggat catggaagtg
    63121 atcctgtcca gtacgactgc acaaaagcat ttttacggaa aattaacagg tgttttactt
    63181 gttgcattaa cgcaaatggc attatacggt attatttttg gtataggata cagtcaattc
    63241 aaagatatgg aaatggtcaa aaatatatta gacggtatct cattggataa cttgattgga
    63301 ccattcttgt ggtattctct tctattcatg atctttggca ttttgatttt ttctgtttta
    63361 gcagctttat gtggctcttt ggtcaacaaa gcagaagata cagcaaaagc aattttgcca
    63421 gtgacttatc tatctctagg cggatacatg cttggattga tactaggagc ttcagatcca
    63481 aacaatatcg ttattcggat cacttcgtat attccattct tatcttcata tattatgccg
    63541 attcgcttag caaatgagac agtcggtatg ggtggagcat tgatttcctt gttggtactt
    63601 gtagccgtaa cattcttatt gatgatcttg tctgcaaaca tgtacaaatc gaatgtactc
    63661 gtttatagcg aaggcggatt atggagttca ttgaaacaat cgatttcaat tatgaggaat
    63721 gaacgaaaaa aagcataaaa caatcctaaa atacagaagg aagctggaca tctattgttc
    63781 ccagcttctt tttgtataga cctattttat ttattttact atattggata tatattcatt
    63841 gaaaatagtg tataacaact ttataatatt gagtatacca atttttggag gcgttttaat
    63901 ggaccgttat ttagatttac tttcagaaaa attcccctca acggaggatg tcatcacgga
    63961 aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga acagagttat ttttgagtga
    64021 tatacatggc gaatttcctg cttttgatca tattctgcgt atcggttcag gaaatcttaa
    64081 agaaaagatc aaagaccttt tttctgaaca aatgacggaa acggatatca gccaattcac
    64141 tatttttacg gcttatccag aatatgcatt aacgactgat tggtacaaag aacaagataa
    64201 atcgcagctg atccatcatt tgatcgatct tttacggttt acgacagtca aatatacccg
    64261 gtcaaaagtt cgaaaaggat tgccgaaaga atatagctat atcattgagg aactcttgta
    64321 tatcgatgac cgtatcaatg gaaaaaaaga ctatgtaaaa aaaatcattg aacaacttgt
    64381 tttgatgaag gaagaagaaa aatttttaac gaaacttgca cagacgatcc aaaaatctgt
    64441 catcgatcat ttgcatatcg ttggagatat ttttgaccga ggaactcaag cagccaaagt
    64501 gatggatcga atcatggatt tttcttctgt agatatccaa tggggaaatc atgatatttt
    64561 gtggattggt gcgtattgcg gctctgaggc atgtttactg acacttttac ggattgcagc
    64621 acgctataat tatttatttg aattagagaa agaatatggg ttgaatttac gtccattgtt
    64681 ttcttttgct catcaaactt atcagaagaa tccagtattt gcgcctaaaa acagtaaaaa
    64741 tctatccgaa agagagcaag aagaattaga aaaaatccat caggcacttt ctatccttca
    64801 attcaaatca gaacatcagt tattggatcg tcgacctgaa tttaaaatgg acgatcgtaa
    64861 attgctggaa aaaatcgact atgaagcatc aacgattgat ttagaaggtc agctctatga
    64921 gttgaaagga acttgttttc aaacgatcca gccagatgat ccaaaaaaat tcttggaaga
    64981 ggaacaaaaa gtgattgata gtttgatgta ttcattccag cactctttga gaatgcaaaa
    65041 acatatgacg tttttgatcg aaaaaggggg aatgtatctc acgaacaatc agcatgtgct
    65101 gtttcatggc tgtatacctg tagacgagaa gggacagctc ttagcatttg aattagacca
    65161 atcttatcgc ggaaaagaac tgctgggctt ttttgaaaaa cagataactg aaagcgcaaa
    65221 agatctgact gcaaaagaag atttagcaac cgatttgatt tggtatgcgt ggagtgggcc
    65281 attttcgcct ttattcggca agagtaaaat ggcgactttt gaacgttatt ttctcacaga
    65341 gcctcatacg cacgtcgaga aaagcaatcc ttattatcac ttgcgagatg aagagtggtt
    65401 cagtgaaaaa attttagcag aattcgacac aaaagaagaa gggtcagcaa tcattaatgg
    65461 gcacacgcca gtaaaagtaa aaaaagggga gtcaccgatc aaagcaaatg gaaaagtatt
    65521 tgtcattgac ggaggtttat ccaaagctta tcaaaaaacg acaggaattg ccggttattc
    65581 actattatgc aattcttatg gtttccaaat cgtgacacat tatccgtttt tgcctattga
    65641 acaacaattt gcaaaaaaat ccgaccaaac aaatgtgaaa aaagtaatcg aacagcaact
    65701 accacgcaaa ctcaatcgtg atacaacaaa aggtatggaa ctgcagcagc agattaccca
    65761 gctcaaaagg ctgttggact atcgaaagga cgattgagaa gaattccagt gaacattgtt
    65821 ccatagactg gagactgaga caaaagtcgt gtcgcagtct ctttttccgg ataaacggtg
    65881 ttcttctgcc aactcctcgg tcacaagtca cactattcaa caaacatgaa aagctgtttt
    65941 ttgaaattgc tccttgtgac tcagagttga acggcaggct cacgacctct tggtataaac
    66001 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tgaaaagcca
    66061 ttttcacaaa atttttctta atactcgaaa ctaaacagct ttttcacaac cccctcagaa
    66121 agtctattaa aattcattca caaagaaatc gaataagcgc tgttcagatg gatgtacacg
    66181 gtgggtaagt tccggatgcc attgaacacc gaggatttta tgcatatcat ctttgctttg
    66241 aaccgcttca acgagtccat cgttagaaaa agcgatggct tttaataaag gtgaaagatc
    66301 tttgactgcc tgatgatggt aagaattcac aaaatatgga tcggggagaa ggttgcttaa
    66361 caaactgtct gaaacaatct tgacttcgtg agtcgtaaat tgcggggcag tgggctgttg
    66421 ttcatgtttt actgaccact ttggatataa tgaaagatct tgataaagtg taccttccaa
    66481 aacgacattc aatagttgga gtccccggca gactgcaaaa attggttttc tttgtttcaa
    66541 tgcttccaag atcagtgcct gttcaaattg gtctcgttga atatttgttt ccgttagctt
    66601 tggatgagga tcttccatgt aaagttgtgg actgacatct tgaccacctg caagaataag
    66661 tttgtcgatt tgtccaatca gttgaggagc tgaatctgga gacatgactg gcagaataag
    66721 aggcaatcct tgagcatcta aaactgcatc cacgaatcct tgcggtgtat agctcacttg
    66781 atttccttgg aaagtatctg ttgctcgaat cagttgattt ccggcaatgc cgatgattgg
    66841 tttcatttgt attcccccta aagtagactt attgtaacaa ggatttaaaa aaaattaaac
    66901 tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg taaagagaaa
    66961 tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat aataaaaagt
    67021 aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa ggggtgagaa
    67081 ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac atactttcaa
    67141 tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca agtgttagaa
    67201 gctttggaac aaatccgaaa agcagaagaa gaaaatgaaa agcgtgctca aacactgcgg
    67261 cagcagctga agatctatgc aacagaaaaa gagcaggaat tggaaacgat ccaaacaaac
    67321 tatcgacaac aactaaaaca gttggtggaa gaaaaagaac aatttgaaat acaagaatta
    67381 gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac tttgaaagat
    67441 caatatcaaa agcatcagag tcaagcgatc ggtgcgatca ttgaaagggt gaaagaaact
    67501 tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa atcaggagag
    67561 gattttacaa gcagtacaag gaatccaaaa cgttgaaatc cgggatctgt atcaaaatag
    67621 aacaaacaac cagtgggtgg agaagtattt tccagatacc acgattattg ataaagaagc
    67681 aaaattaagc gctttaggta atcggttgaa agacatccaa gaagctatcc aattcataca
    67741 acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac tttccttaca
    67801 agatttggaa gccaattatt ccgaagaaaa tttcataaat aaattgaaag aagtccttga
    67861 tttaaaagaa aagtgggaac aactaactga agaacgtgat cgtttagaag aagaggaaga
    67921 atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa gtaagacgac
    67981 catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg aagaactgag
    68041 taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag tttatctttc
    68101 gtatgttgct ttaaaaaaat cagttgctgt tatagaagaa attgctggga aatacgggtt
    68161 caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaactgcaag agaccaagca
    68221 gcaattatcc attgttcacg agcagcaaaa gaagataacg acagcgttag gtcagtgcag
    68281 tggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg cagaacgaga
    68341 aaaaataaaa gaacggttta tccatgcgac ttatctgatc gttttgcaag gttgggtaga
    68401 tgcagaagaa aaacagaatt tgctgcatgt gttaaacgaa accgtatcag aagaagatat
    68461 atacgtcagt ttcgaaacac caaactcaga agagattcag caggaagtcc ctactaaatt
    68521 gaaaaaccat ccgcttgtcg aaccttttga attgctgact gaaatgtaca gtctgccaaa
    68581 atatgaagaa gtagatccaa caccttggat gacaccattt tatcttgtct tcttcgggat
    68641 gatggtcgca gatgttggtt atggtttgct gatgctcatt gggacaatac tagctcaaaa
    68701 actacttgtt ctaccacgcg ggatgaaacg tttcgtaaaa ttctttgaga ttctctcgat
    68761 accatcgatc atctggggat tgatctacag ttcatttttc ggacagtcat taccaaaaga
    68821 actgtttggc atccgcttgc ctttcccaat cctatctacg acggatgacg tcaatacgat
    68881 tttgatttta tccgtgattt tcggtctgat acagattttg gttggattat ttgtggcagc
    68941 aaaggaaaat atcaggagaa aagattactt gagtgctgtc agtgatggat ttgcctggca
    69001 agggatccta attgggatcg ttattgcgtt ggttggtgcc atgttgataa aaaatacagc
    69061 atttgtttac cttggcgctg ggctatcgat cattgctgcc ttatgtatct tgatcgttcc
    69121 gattatccaa acgccgtcta aagtcaaagg tgctgccaaa ggtgcgtata atctttacgg
    69181 actgaccggc tatatcggtg atttagtcag ttatacacgt ttgatggcat tgggtatttc
    69241 aggcggaagt attggagcgg cattcaatat gctagttgct ttcatgccgc cagctgctcg
    69301 attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt ttttgacact
    69361 gctaagtgcc tatgtccatg gcgcacgatt gcagtatgtc gaattttttg ggaagtttta
    69421 tacaggaggc ggacgtgcct ttgatccgct gaaaacggcg gaaaaatacg tcaacatcaa
    69481 tcacaagaaa aaaaacaata aataattgga ggatttttat ataatgatgg attacttaat
    69541 tagtcaaaat ggtggaatgg tatttgcggt attagcgatg gcaacagcga cgattttttc
    69601 agggatcggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag cattgacaac
    69661 aagtcaaccc gaaaaattcg ggcaggctct gattctgcaa ttgcttccag gcacgcaagg
    69721 tttgtatgga ttcgttattg cctttttgat ttttatcaac ttaggaaatg acatgtcagt
    69781 tgtacaagga ctgaactttc taggtgcttc tctgccgatt gcttttacag gcttgttttc
    69841 aggtattgcc caaggaaaag tcgcagcggc aggtattcaa attttagcga aaaaacctga
    69901 acatgctaca aaaggaatca tttttgctgc gatggttgaa acatacgcga ttttaggttt
    69961 cgttatttcc ttcctattag tgttgaatgc ctaagcataa aggaggaaaa gcagatgaac
    70021 gccattgaaa aaatcatctc tcagatgaac gaagcagcag agcaggaaag agcggcgtta
    70081 gaacaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac gcaagttgaa
    70141 acagaacatc aaaaacaaaa agaaaaacaa atcgaattac tcgaaaaaaa ataccgtcag
    70201 ctaaggaatc gccagcaagt agaagtacga caagaaaatt tgaatgcgaa gcaggaattt
    70261 ttaagacgat tgtttgcaga tgcggtaact gaaatggaaa attgggatga atctgaacaa
    70321 atccagttta tcaaaaatgc attgtattca ttacctttga caggaaaagt agcattcatt
    70381 gctggagaaa aatcagcagc ctatctttct cagacgttgt tggatgaatg gaacaatgaa
    70441 cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg ttttctgata
    70501 aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga tatccaagga
    70561 acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag tgtatggaat
    70621 accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc aatgagacat
    70681 ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg aaatccacga
    70741 tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat cttttaaagg
    70801 agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa atcgtttgga
    70861 tctatacgat gcgttatact ttccataatc tgaaggtttt gacaaaagct gagatgacaa
    70921 atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa accttgaagg
    70981 atgccattca tacgcaagtt tcaaatgaac ttcctgaaag cattatggac agtatacgag
    71041 aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg atttatgatc
    71101 gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa gaattgctga
    71161 cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga ggaatattgc
    71221 agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt atcgaaaaag
    71281 aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt ctattagaga
    71341 cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatattgat ctcgtaaagc
    71401 ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa acccaggctt
    71461 tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc aaaaaccttc
    71521 gtttactggt tatcggcaaa agaagccatt tcgaatcaga aacaatcaga aagcgggtga
    71581 gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt tttgccattc
    71641 aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg tcaggcaatt
    71701 gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg tgcagcattg
    71761 agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt attgattccc
    71821 aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt agaaaaagca
    71881 gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat tggaaaaatc
    71941 ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag tatccaagac
    72001 atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg tggcgatgtg
    72061 gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc ggttcgctct
    72121 actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat gtttgacgga
    72181 atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt agggcgcgga
    72241 gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac agtagaagcc
    72301 ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa agttataaaa
    72361 cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga aaaaggttca
    72421 ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa aaatttgacg
    72481 atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt aaacccggat
    72541 gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac aaaaggagga
    72601 gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca tcaaattgca
    72661 aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg aaatgagatg
    72721 acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga atcgttgatg
    72781 gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg agaagcttct
    72841 atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga cgttgctatc
    72901 atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg acgtttggaa
    72961 gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc ggaatactat
    73021 gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag tataaccgca
    73081 atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca aaatacattg
    73141 cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag acattttcct
    73201 tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa gtatatggat
    73261 catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat tttgcaggaa
    73321 gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc agataatgac
    73381 cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca aaatgctttt
    73441 gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa gatgatcttg
    73501 acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag tgagatcatg
    73561 gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc ggaaacagaa
    73621 ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac gatcgtttca
    73681 gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg tcggtccatt
    73741 gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag tccgtatgca
    73801 aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag caatggtcca
    73861 aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat ttttaggcca
    73921 tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg gtttgggacg
    73981 accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta acggagaagt
    74041 aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta tctctgcaat
    74101 cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg gctctggtct
    74161 tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg attcatcaga
    74221 tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg aatttttcat
    74281 ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga atttagcaaa
    74341 cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg cagaatatct
    74401 tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca actatgcaga
    74461 ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag gatatcctgg
    74521 gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc gcggactaaa
    74581 aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa ctcatccaat
    74641 ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag agctttacaa
    74701 gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa aagataaggg
    74761 aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc tttttgcagc
    74821 ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat ctgctttatc
    74881 tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat atgtcaatca
    74941 aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg aactgctatc
    75001 gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca aatatctgcc
    75061 agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca tggaattgac
    75121 tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa aagataagca
    75181 agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc tgcgtcaaga
    75241 aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg cttcgatcac
    75301 ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt tgtctatcgt
    75361 agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg atgaacagct
    75421 gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg atcggtcaat
    75481 cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg aaaaaacttg
    75541 tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat tagaatatat
    75601 gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg aagaaaacga
    75661 gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg actaagtggt
    75721 atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa gaaacgtttg
    75781 ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg cggcggccta
    75841 cttaccctac cattttttag taatagtggg catgggacaa attttatcga tgcgcttttc
    75901 actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc agagcattgg
    75961 aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct tggttttatg
    76021 atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag cacacgtatt
    76081 atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct catgctttac
    76141 atcctaaaat tggcctttgt gatccaattg attggtacgg ttgctttatc ttttgtattt
    76201 gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc aatctctagt
    76261 ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca gcaaaatgtt
    76321 tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt catcgtatgg
    76381 cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa aatcgcatta
    76441 accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac agagcgcaat
    76501 ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt ttttatgagt
    76561 gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag ccatgcaggt
    76621 ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac ggccggcgga
    76681 ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt gaaaggacgt
    76741 acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg ggcacttact
    76801 ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc ggtgactgaa
    76861 acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc tgcgtttggt
    76921 acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa attgatcatt
    76981 atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc tctgatgaca
    77041 aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat gattggataa
    77101 tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa taatttttct
    77161 tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac tacccgttgt
    77221 tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg attcctttat
    77281 ttatcaatag gttataattc atattttttt attttttctt ttacgatact tagtccagaa
    77341 gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc tggttgcgca
    77401 cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa gtcattctca
    77461 attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg gacacgattc
    77521 gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt tgtaagactt
    77581 aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt gatgacttcc
    77641 acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat cttgctttgg
    77701 acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa ttttgtcatt
    77761 ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat gatcgtatgt
    77821 tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag accttctttt
    77881 tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc tataggatta
    77941 ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc ctgaatcaat
    78001 atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa atgatataac
    78061 tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct atcacctgga
    78121 gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac aatagataga
    78181 gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt agtcaattga
    78241 aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg gttattacta
    78301 gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact aaccagacca
    78361 aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc aaagacattg
    78421 ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc aattccatat
    78481 ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag ctctctttgg
    78541 gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc taattgttga
    78601 atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca agttaacggg
    78661 taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat accatacaat
    78721 tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga attgtctatg
    78781 aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc gattcaagta
    78841 atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc tcgattggtg
    78901 gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc ttatttgggg
    78961 gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca tcagtatcat
    79021 ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact acccaaagca
    79081 ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc gctggcaaca
    79141 gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg tcaacaagca
    79201 ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt tgtttacgat
    79261 attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta tcctaaaata
    79321 acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat catttgtacg
    79381 ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga cgcacatatc
    79441 aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt gatggaaact
    79501 tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga cctgcttata
    79561 gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg cgaattagtt
    79621 tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga cgcagttggt
    79681 ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga gaaaggggct
    79741 ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg ttcaaaggaa
    79801 atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg caaaaaattg
    79861 ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa gaatttgagc
    79921 ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca ttagcaacag
    79981 catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt tctactcaaa
    80041 gcggtaatct atttgtgaca agaattaaag attattatta catggatttt cctagtatta
    80101 tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct aagattaaag
    80161 aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg gtggctaaat
    80221 tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc atcgtgacag
    80281 cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta acaatcaatg
    80341 aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca gaaagaatga
    80401 ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta gaatgtgaat
    80461 taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa ggtacctcgt
    80521 ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca accttatagc
    80581 tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt ttgcgtctca
    80641 ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca tactaactat
    80701 cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta agaaggcgca
    80761 gtgccaggta atccttttaa gtcagtaatc tcaagaccag cagctttatg ttgctcgtgt
    80821 aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc tagatcatac
    80881 gcaccatgat catagttgta agtcaactct aattcatagt catccccagg aagtgtcaaa
    80941 taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat
    81001 gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca tgtgtgagcc
    81061 attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa gaagtaagga
    81121 aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata gaaaataaaa
    81181 ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa gcaatttgct
    81241 gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca gattgcagta
    81301 atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg agaagagacg
    81361 aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc agaaatttct
    81421 tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac ttattattat
    81481 aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc aactgaaaag
    81541 accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc
    81601 caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa agcgagtaga
    81661 tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg cttcagctac
    81721 accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa gagaaacggc
    81781 atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt tctcgttatc
    81841 tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc
    81901 cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt
    81961 tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg
    82021 ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt caggtgttcg
    82081 atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg
    82141 attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga cacggcgtgc
    82201 cattacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga tgattttacc
    82261 tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat tatcttgaaa
    82321 aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag tgcataaaac
    82381 gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt ttgttacttc
    82441 tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga tagacataat
    82501 ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat ggctggcaaa
    82561 ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat caaccatttc
    82621 ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag caacaagctg
    82681 actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg
    82741 atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag aattcacagc
    82801 agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt cttggactaa
    82861 ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg
    82921 tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa agtaagtgcc
    82981 caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt caagaaatgg
    83041 cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca actcgtgtgc
    83101 gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat ggaagtacac
    83161 ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca tgccactcat
    83221 ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg taagatcaaa
    83281 tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca ttcgatctgc
    83341 atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt tttggatcac
    83401 actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat acacttctgt
    83461 ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga agatatgttc
    83521 aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac ataaagtcac
    83581 cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa aatttcgaga
    83641 aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag taaaacggct
    83701 atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc gtcgtagacg
    83761 ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat ggatatagtt
    83821 atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat aaggggttgt
    83881 cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg ttgttccatt
    83941 gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca cttgcagaat
    84001 acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa aggggttggt
    84061 caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag ccatttgtac
    84121 caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgccca cgtttttcct
    84181 gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc cgacgctgag
    84241 cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga caataagaat
    84301 gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc aataggagca
    84361 tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg aaaatcaaag
    84421 aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt ctgatcactg
    84481 gagcgtaacc tgcatcttcc agtgtttgga tcccgtcact tagaaaagga gttcgaacat
    84541 ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag atttgctggt
    84601 gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag tcgttttttt
    84661 gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct ttgtataaat
    84721 tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa aaaggttttc
    84781 tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg tagctatgag
    84841 aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa tcaaattgcg
    84901 tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga aagggcaaat
    84961 ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg agacgggcga
    85021 tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga tccgtgcagg
    85081 agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat cctatcgaac
    85141 accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga
    85201 ccgtcccaca gcagggaagg ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat
    85261 cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa gtttgatttt
    85321 ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct gattggaaat
    85381 tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag cacacgcgat
    85441 cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga tccaaaacta
    85501 tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata ctgacttgaa
    85561 tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt catggtatgc
    85621 tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg aagatactcc
    85681 acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag acgcgatcga
    85741 ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg atgatctgtt
    85801 tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact tgcaagaaat
    85861 cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg aagaagattt
    85921 aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat tttctgactc
    85981 tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat cagcctatca
    86041 gaataaggag gtgtgacacg attttt
//
LOCUS       DAYYHS010000002        76272 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000002 DAYYHS010000000
VERSION     DAYYHS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.002"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHA6A_000090"
     CDS             complement(<1..300)
                     /locus_tag="ACHA6A_000090"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8968332.1"
                     /translation="MFQILKLSKNEIRHSNFLAFLFAPNETHNLSDEFFKMFLKRYID
                     SNDDTKAAINYFDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESG"
     gene            complement(264..389)
                     /locus_tag="ACHA6A_000091"
     CDS             complement(264..389)
                     /locus_tag="ACHA6A_000091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968333.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            complement(592..1071)
                     /gene="rlmH"
                     /locus_tag="ACHA6A_000092"
     CDS             complement(592..1071)
                     /gene="rlmH"
                     /locus_tag="ACHA6A_000092"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8968334.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1499..2788
                     /locus_tag="ACHA6A_000093"
     CDS             1499..2788
                     /locus_tag="ACHA6A_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8968335.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2827..3432)
                     /gene="ytpR"
                     /locus_tag="ACHA6A_000094"
     CDS             complement(2827..3432)
                     /gene="ytpR"
                     /locus_tag="ACHA6A_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8968336.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3637..4101)
                     /locus_tag="ACHA6A_000095"
     CDS             complement(3637..4101)
                     /locus_tag="ACHA6A_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8968337.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4123..4443)
                     /locus_tag="ACHA6A_000096"
     CDS             complement(4123..4443)
                     /locus_tag="ACHA6A_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8968338.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4587..5666)
                     /gene="pepA"
                     /locus_tag="ACHA6A_000097"
     CDS             complement(4587..5666)
                     /gene="pepA"
                     /locus_tag="ACHA6A_000097"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8968339.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5866..6216
                     /locus_tag="ACHA6A_000098"
     CDS             5866..6216
                     /locus_tag="ACHA6A_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8968340.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6439..7386
                     /locus_tag="ACHA6A_000099"
     CDS             6439..7386
                     /locus_tag="ACHA6A_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8968341.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7443..8861)
                     /gene="pepV"
                     /locus_tag="ACHA6A_000100"
     CDS             complement(7443..8861)
                     /gene="pepV"
                     /locus_tag="ACHA6A_000100"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8968342.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            9010..9855
                     /locus_tag="ACHA6A_000101"
     CDS             9010..9855
                     /locus_tag="ACHA6A_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8968343.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9859..10299
                     /locus_tag="ACHA6A_000102"
     CDS             9859..10299
                     /locus_tag="ACHA6A_000102"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8968344.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10349..10543
                     /locus_tag="ACHA6A_000103"
     CDS             10349..10543
                     /locus_tag="ACHA6A_000103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968345.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10679..11356
                     /locus_tag="ACHA6A_000104"
     CDS             10679..11356
                     /locus_tag="ACHA6A_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8968346.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(11764..12453)
                     /locus_tag="ACHA6A_000105"
     CDS             complement(11764..12453)
                     /locus_tag="ACHA6A_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968347.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12428..14557)
                     /locus_tag="ACHA6A_000106"
     CDS             complement(12428..14557)
                     /locus_tag="ACHA6A_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8968348.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14605..15942)
                     /locus_tag="ACHA6A_000107"
     CDS             complement(14605..15942)
                     /locus_tag="ACHA6A_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8968349.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(16194..16553)
                     /gene="rplT"
                     /locus_tag="ACHA6A_000108"
     CDS             complement(16194..16553)
                     /gene="rplT"
                     /locus_tag="ACHA6A_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8968350.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16606..16806)
                     /gene="rpmI"
                     /locus_tag="ACHA6A_000109"
     CDS             complement(16606..16806)
                     /gene="rpmI"
                     /locus_tag="ACHA6A_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8968351.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16878..17402)
                     /gene="infC"
                     /locus_tag="ACHA6A_000110"
     CDS             complement(16878..17402)
                     /gene="infC"
                     /locus_tag="ACHA6A_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8968352.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(17446..17571)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17722..18354
                     /locus_tag="ACHA6A_000111"
     CDS             17722..18354
                     /locus_tag="ACHA6A_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8968353.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18356..19261)
                     /locus_tag="ACHA6A_000112"
     CDS             complement(18356..19261)
                     /locus_tag="ACHA6A_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8968354.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19346..20005)
                     /locus_tag="ACHA6A_000113"
     CDS             complement(19346..20005)
                     /locus_tag="ACHA6A_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8968355.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            20321..21265
                     /gene="mvk"
                     /locus_tag="ACHA6A_000114"
     CDS             20321..21265
                     /gene="mvk"
                     /locus_tag="ACHA6A_000114"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8968356.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            21258..22235
                     /gene="mvaD"
                     /locus_tag="ACHA6A_000115"
     CDS             21258..22235
                     /gene="mvaD"
                     /locus_tag="ACHA6A_000115"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8968357.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            22248..23333
                     /locus_tag="ACHA6A_000116"
     CDS             22248..23333
                     /locus_tag="ACHA6A_000116"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8968358.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23330..24394
                     /gene="fni"
                     /locus_tag="ACHA6A_000117"
     CDS             23330..24394
                     /gene="fni"
                     /locus_tag="ACHA6A_000117"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8968359.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24643..25446)
                     /locus_tag="ACHA6A_000118"
     CDS             complement(24643..25446)
                     /locus_tag="ACHA6A_000118"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8968360.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25466..26308)
                     /gene="kduI"
                     /locus_tag="ACHA6A_000119"
     CDS             complement(25466..26308)
                     /gene="kduI"
                     /locus_tag="ACHA6A_000119"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8968361.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26324..27331)
                     /locus_tag="ACHA6A_000120"
     CDS             complement(26324..27331)
                     /locus_tag="ACHA6A_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8968362.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27338..27982)
                     /locus_tag="ACHA6A_000121"
     CDS             complement(27338..27982)
                     /locus_tag="ACHA6A_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8968363.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(28082..28840)
                     /locus_tag="ACHA6A_000122"
     CDS             complement(28082..28840)
                     /locus_tag="ACHA6A_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8968364.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28967..30118)
                     /gene="fucO"
                     /locus_tag="ACHA6A_000123"
     CDS             complement(28967..30118)
                     /gene="fucO"
                     /locus_tag="ACHA6A_000123"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8968365.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(30200..30511)
                     /gene="rhaM"
                     /locus_tag="ACHA6A_000124"
     CDS             complement(30200..30511)
                     /gene="rhaM"
                     /locus_tag="ACHA6A_000124"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8968366.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30513..31358)
                     /gene="rhaD"
                     /locus_tag="ACHA6A_000125"
     CDS             complement(30513..31358)
                     /gene="rhaD"
                     /locus_tag="ACHA6A_000125"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8968367.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31372..32628)
                     /gene="rhaA"
                     /locus_tag="ACHA6A_000126"
     CDS             complement(31372..32628)
                     /gene="rhaA"
                     /locus_tag="ACHA6A_000126"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8968368.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32625..34085)
                     /gene="rhaB"
                     /locus_tag="ACHA6A_000127"
     CDS             complement(32625..34085)
                     /gene="rhaB"
                     /locus_tag="ACHA6A_000127"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8968369.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            34238..35107
                     /locus_tag="ACHA6A_000128"
     CDS             34238..35107
                     /locus_tag="ACHA6A_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8968370.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(35233..36132)
                     /locus_tag="ACHA6A_000129"
     CDS             complement(35233..36132)
                     /locus_tag="ACHA6A_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8968371.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(36297..37121)
                     /locus_tag="ACHA6A_000130"
     CDS             complement(36297..37121)
                     /locus_tag="ACHA6A_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8968372.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(37132..38427)
                     /locus_tag="ACHA6A_000131"
     CDS             complement(37132..38427)
                     /locus_tag="ACHA6A_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8968373.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38443..38922)
                     /locus_tag="ACHA6A_000132"
     CDS             complement(38443..38922)
                     /locus_tag="ACHA6A_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8968374.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(38928..39956)
                     /locus_tag="ACHA6A_000133"
     CDS             complement(38928..39956)
                     /locus_tag="ACHA6A_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8968375.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(39973..41079)
                     /locus_tag="ACHA6A_000134"
     CDS             complement(39973..41079)
                     /locus_tag="ACHA6A_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8968376.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(41308..43905)
                     /gene="adhE"
                     /locus_tag="ACHA6A_000135"
                     /gene_synonym="adhC"
     CDS             complement(41308..43905)
                     /gene="adhE"
                     /locus_tag="ACHA6A_000135"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8968377.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(44210..44590)
                     /gene="yajC"
                     /locus_tag="ACHA6A_000136"
     CDS             complement(44210..44590)
                     /gene="yajC"
                     /locus_tag="ACHA6A_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8968378.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(44687..45832)
                     /gene="tgt"
                     /locus_tag="ACHA6A_000137"
     CDS             complement(44687..45832)
                     /gene="tgt"
                     /locus_tag="ACHA6A_000137"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8968379.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            46050..46748
                     /locus_tag="ACHA6A_000138"
     CDS             46050..46748
                     /locus_tag="ACHA6A_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8968380.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(46916..49555)
                     /locus_tag="ACHA6A_000139"
     CDS             complement(46916..49555)
                     /locus_tag="ACHA6A_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8968381.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(50174..51205)
                     /gene="queA"
                     /locus_tag="ACHA6A_000140"
     CDS             complement(50174..51205)
                     /gene="queA"
                     /locus_tag="ACHA6A_000140"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8968382.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            51398..52573
                     /locus_tag="ACHA6A_000141"
     CDS             51398..52573
                     /locus_tag="ACHA6A_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8968383.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            52577..53218
                     /locus_tag="ACHA6A_000142"
     CDS             52577..53218
                     /locus_tag="ACHA6A_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8968384.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            53215..54132
                     /locus_tag="ACHA6A_000143"
     CDS             53215..54132
                     /locus_tag="ACHA6A_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8968385.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            54129..54800
                     /locus_tag="ACHA6A_000144"
     CDS             54129..54800
                     /locus_tag="ACHA6A_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8968386.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(55341..55829)
                     /locus_tag="ACHA6A_000145"
     CDS             complement(55341..55829)
                     /locus_tag="ACHA6A_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8968387.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(56030..56296)
                     /locus_tag="ACHA6A_000146"
     CDS             complement(56030..56296)
                     /locus_tag="ACHA6A_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8968388.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(56296..56559)
                     /locus_tag="ACHA6A_000147"
     CDS             complement(56296..56559)
                     /locus_tag="ACHA6A_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8968389.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            57080..57220
                     /locus_tag="ACHA6A_000148"
                     /pseudo
     CDS             57080..57220
                     /locus_tag="ACHA6A_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(57362..59185)
                     /locus_tag="ACHA6A_000149"
     CDS             complement(57362..59185)
                     /locus_tag="ACHA6A_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8968390.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(59783..62488)
                     /locus_tag="ACHA6A_000150"
     CDS             complement(59783..62488)
                     /locus_tag="ACHA6A_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8968391.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(62794..63741)
                     /locus_tag="ACHA6A_000151"
     CDS             complement(62794..63741)
                     /locus_tag="ACHA6A_000151"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8968392.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            63931..64200
                     /gene="rpsN"
                     /locus_tag="ACHA6A_000152"
     CDS             63931..64200
                     /gene="rpsN"
                     /locus_tag="ACHA6A_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8968393.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(64376..64738)
                     /locus_tag="ACHA6A_000153"
     CDS             complement(64376..64738)
                     /locus_tag="ACHA6A_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8968394.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(64749..65870)
                     /gene="alr"
                     /locus_tag="ACHA6A_000154"
     CDS             complement(64749..65870)
                     /gene="alr"
                     /locus_tag="ACHA6A_000154"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8968395.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(65886..66236)
                     /gene="acpS"
                     /locus_tag="ACHA6A_000155"
     CDS             complement(65886..66236)
                     /gene="acpS"
                     /locus_tag="ACHA6A_000155"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8968396.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(66366..67877)
                     /locus_tag="ACHA6A_000156"
     CDS             complement(66366..67877)
                     /locus_tag="ACHA6A_000156"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8968397.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(68199..69551)
                     /gene="murF"
                     /locus_tag="ACHA6A_000157"
     CDS             complement(68199..69551)
                     /gene="murF"
                     /locus_tag="ACHA6A_000157"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8968398.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(69704..70780)
                     /locus_tag="ACHA6A_000158"
     CDS             complement(69704..70780)
                     /locus_tag="ACHA6A_000158"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8968399.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            70976..72721
                     /locus_tag="ACHA6A_000159"
     CDS             70976..72721
                     /locus_tag="ACHA6A_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8968400.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(73001..73438)
                     /locus_tag="ACHA6A_000160"
     CDS             complement(73001..73438)
                     /locus_tag="ACHA6A_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8968401.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(73422..74795)
                     /locus_tag="ACHA6A_000161"
     CDS             complement(73422..74795)
                     /locus_tag="ACHA6A_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968402.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(75117..75605)
                     /locus_tag="ACHA6A_000162"
     CDS             complement(75117..75605)
                     /locus_tag="ACHA6A_000162"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8968403.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(75627..76229)
                     /locus_tag="ACHA6A_000163"
     CDS             complement(75627..76229)
                     /locus_tag="ACHA6A_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8968404.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        21881 a        16293 c        13938 g        24160 t
ORIGIN      
        1 tccagactcc gaagacagaa ttttattttc gatgcacacc actattttat ttttttccga
       61 taccaataaa atatcaatat tcttattttc tctgtaaaca attaaatctt catagctatt
      121 aagtaaagca tcaaaataat tgatagcagc tttggtatca tcattagaat ctatatacct
      181 tttcaaaaac attttaaaaa actcatcaga taaattgtgg gtttcatttg gagcaaacaa
      241 aaaagctaaa aaattactat gtcttatttc atttttagat agcttaagta tctgaaatat
      301 atttgttttc ccagtccatt tagatagttt tcttcaatat catccatatc ctctaataga
      361 ttttttaaac actttttttc atctatcatg ttttttctcc ttgccattat tctaactaaa
      421 attttacgta tttgtttcta tgtctacatt atctttttaa cagtatctat ttttcttttg
      481 caacttactt ggctaccaaa gcaaataaaa aagttattta taaatttctc cctaccacta
      541 cctattgcta ttatttaaaa tagggataat tttttctctt aaaaaatatc attatttgtg
      601 gtatggagcc cccgcattga tccggaaggc acggtagatt tgctcgacaa ggatcagacg
      661 catcaattga tgtggatacg tcatttttcc aaaagagatc tgagcattgc ttcttttcat
      721 gacggcttcg ctgagtccta aagagccgcc gataacaaaa acaaggtggc tttttccttg
      781 gatacctaat tggtcgattt gtttagcaaa agattcactt gtaggatttt tcccttcaat
      841 tgctaaggca tagacgtatt cttgttcttt gattttcgct aagatccgtt ccccttcttt
      901 ttcttttact tgtctcattt gcgcttcgct taaattctcc ggggcttttt cgtcaggaac
      961 ttcgatcaat tcgatttttg cataggcatt cagccgtttg acatactcat tgataccttg
     1021 gatcaggtat ttttctttta attttcctac tgagataatt ttgatattca tgttttctct
     1081 cctttggtct tcatgctcac tttgtttatt ataatcaatt ttttcgtcta tatgtttatt
     1141 ttggctattt ttgttaaaaa gatttcccca acagttatgc acaacatcca caggtttatc
     1201 cacagaaaac agtgttattt taccttattt catcacattc attaggcaaa catacgtttt
     1261 ataagaattt atccacttta tacacaggct gtggatatgt tttatccctt atttttccaa
     1321 tcctatccgc gtagttttac acagtatctg tggaaaaaga aaatcaaata ttttcatctg
     1381 aaaacaatga tttttcctaa agtttttcat aaaaagttca cagtctcaaa gtagactaaa
     1441 gtcaacaaat aagactatac taacagtaga taaatgaatg taattggagg aggaacacat
     1501 ggatagaaaa aatgtgacac caaagatgaa aaaaagtaaa aataatagcc tctggcgtaa
     1561 gttaggtctg gggcttgttg gagggatcgt tggaggacta ctgacagctg gcatttttta
     1621 tgcagcgatg ggcactggca acaatagttc tactgctaca agcggcaatc agaattctgc
     1681 cggtgaaaca gttgtggaaa atgtcaaagt caatgtagat tctgatatca ccaaagcggt
     1741 agataaagtt caagacgctg tcgtttctgt tatcaactta caaagtcaaa accaatcaag
     1801 cggttttggc ggactattcg ggcagcaaga agaaagtagc agctcagatg atagtaattt
     1861 ggaagcttat agtgaaggaa gcggcgtgat ctacaaaaaa gacgggaata ctgcttatgt
     1921 agtcacgaat aatcacgtag tagatggcca gcaaggtctt gaagtcttga tgaaagacgg
     1981 tacgaaggta aaagctgaat tagttggaac agatgcttat tcagatttag cggtgttgaa
     2041 gatcaattca gacaaagtcg aaacagttgc ctcttttggt gactcaagtg ctctgaaggt
     2101 tggtgagcct gcgattgcga ttggttcccc actaggatct gaatatgcaa attctgtcac
     2161 ttcaggaatc atttcttcct tgaaccgaca agtgacaagt acgaatgaat cgaacgaaac
     2221 agtcaatatc aatgcgatcc aaacagatgc agcaatcaac cctggaaatt ctggtggtcc
     2281 tttagttaat atcgaaggac aagttatcgg tatcaactca agtaagatcg caagtacttc
     2341 tgaatctact tctaatgtaa gcgtagaagg aatgggattt gctattccaa gtaacgatgt
     2401 agtgaatatc atcaatcaac tggaaaaaga cggaaaagtg acacgtccag ctctaggtat
     2461 cacaatggtc gacttatctg ccgtttctac tcagcaacaa gaacaaatct tgaagattcc
     2521 ttcatctgta actaacggtg tcatcgtgac aaatgtccaa acagctacac cggctgaaaa
     2581 agctggattg aaacaatacg acgtcattac taagatcgat gacaaagaag tttcttctgg
     2641 tgtagaactt caatctgctt tgtataagaa aaaagtggga gattctgtca aaatcactta
     2701 ctatgaaggc tcgaaagaaa aaacaactac tgttgaattg acgatcgatc aatcggcatt
     2761 aaaacaaaat caaaatagtg aaaattaata aaaaagaggt tgtgacaaag acttgtcaca
     2821 acctctttat tttggaaatg cttctccgac tacggcatcc tcaggcaata ctaagattcc
     2881 tttttttgct ggagcatttg gcaaatgcaa ttcacgagcg gaacagatca ttccatagct
     2941 atctacccct cgcaaagttc ctggccagat catcattcca tctggcatca tagctcctgg
     3001 ttttgcaacg acaacttttt gtccagcttc gatattgggt gcaccgcaaa cgatttgcaa
     3061 tactgtacca ttgtctactt ctgtctcagt aatcgataaa tggtcagaat caggatgtgg
     3121 gacgcatgtt tttacgtacc cgacaacgat cttcggttca tgatcagctt caagctgttc
     3181 ttcgaatcct gcttctttga tcaatcgatt caatttcgct aattcctcat ctgttaagct
     3241 tacttggccg ttcccttcga ttgttagatg atcggagata tggaaaaagt tccaagcaac
     3301 aacttgacct tgttcatctt tgatccgagc aacattccat ttgcgttcaa cagtgttttc
     3361 tgttccttta tcgtcggcta taataaccat caaggtgtct ccgacgtgtg ctttgttata
     3421 agcaaaaatc atgttctttc ctccattatc ctttgctgag atttatctta acaaaaaatt
     3481 gcattcactg catgaaattt ttttcatgct ttatttccga agaagcctct tctggaacac
     3541 catttattcg taacaaggga ctgtgacaag acttttgtca cagtcccttt actgttttag
     3601 ttaattgatt aaaaaggtcg tacaagttcc acttgcttaa ttacttggct gttcttcttc
     3661 gtcagaaacg atcaagacat cacaaggtga atgacgtatc acgtagctgg ctacacttcc
     3721 tgtcataaag cgttcgactg catttagacc tgactgccca accatgatta gatcgatctc
     3781 atattttttt ggcaatttga ctgccatgga ttcacgaaca gaaccatatg caataattcc
     3841 ttctatattt gtgtaatcaa cagatttccc atattcttta cattcttcga tcatttcctt
     3901 ggctgtttca gtttgctgat cgactagatt ttgatcaata ggtgcatagc ccataaagtt
     3961 ataaaattgc tgatcgatta cgtttgctac atatactttc ccattattac ggcgagccac
     4021 ttctaccgct tttttgaaag cagcaagtgc ttgttcacta ccgtcgatac ctactaaaat
     4081 attcttatat tcttgattca tcttgacccc tcctaacaga tgtcagcctt ttgttacact
     4141 ttctaggaac gattcgattt cctgttttgt tttacgatct ttattcacta gacgacctaa
     4201 ttcttttccg tcttggatca cgacaaaact tgggatacca aaaatgttcc attcagcggc
     4261 tacatctatg tacttgtcac ggtctacttc aataaattgc caatcagaaa agtcattttc
     4321 aatttcaggc atttgcggtt tgataaaacg gcagtctccg caccagccgg cagtaaagaa
     4381 aaatacactc tttccagttt ctacgtagcc tgctaattct tctaaactat ttgggataat
     4441 cattctttgt cctctacttt ctgtttatgt atttttcaat taaattatac acctttcttt
     4501 cgaactaaca aacattgatc ctctttttat aaaaaaaatc tggcaaaaag caatcttttg
     4561 ccagatttta tagtttcact atcgcattat tttccgtata cgatcgtatt tactgttgat
     4621 ttatccaaac ctttcagtgt ttgaatcaac atttctcgtg cagcttcaaa atcgcgaata
     4681 ctgaacatcg tttgatgcgt atggatataa cgtcccacta cgccaatcac tgtacttggg
     4741 attccttcgt tttgtgtatg agcagcacct gcatctgtac cgccttttga aacaaagtat
     4801 tgataaggga tattgtttgt ttcagcgata tccagcaagt actcgcgcaa acgaggaagc
     4861 atgatcaaac ctggatcttg gatacgcatc aatgtacctt ctcctaaatg tccgaatgtg
     4921 ccatttttcg tcactgtatc atctgctgca gagcagtcta ctgcaaagaa cagatctggc
     4981 ttgaatttat tgacagaagc tttagaacca cgcaagccta cttcttcctg gacattcgcg
     5041 cctgcaatca atgtatgacc taatgtttca ttttgcaacg cttctaaagc ttctaatacc
     5101 attgtacagc catagcggtt gtcccatgat ttactgataa tattctttcc gttggctgtt
     5161 ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa ggacaccgta actcattgcc
     5221 tcttctcttg attcaaaccc agcatcgaac aaaatatcgg ttacttctac tgatttttgt
     5281 ccgcttgttc ctcgcaataa atgtggggga acggaagaag aaatacatgg ataattctta
     5341 ccagtagagg ttttaagtgt aaaacgctga gcagaaacaa cgtaaggatt ccagccgcct
     5401 agaggtacga ctttaaaaag cccgttttct tggatttgtg tcagcatgaa tccgacttca
     5461 tccatatgtg ctgcgaccat tacacggggt gcatccgctt cttcatgatg gcgcagacca
     5521 aaaatgccgc ccaaaccatc taactgtaac tcatctacca gcggttccat atgactttcc
     5581 atataagcgc gaacatcttg ttcaaaaccg cttgttcctt gtaattctgt taactctttg
     5641 attcgttgaa atgttttttc ttccatatgc tattcgtcta aatttcagac taaactcctc
     5701 tcagacttat attgatctgc ggataaaata tgattttttc tgcagaccga tcgtatttat
     5761 tatagcttat tttttctggt agcggttatt tatttcaaag aaaatatgag agtgtggtaa
     5821 aataagcaca aggagtaaca agtgagaaga aggaggtatg tccacatgta tgaagaaagc
     5881 gaactaagct attttaaagg cgggttagca gttggaatcg gtctaggatt attgagcggg
     5941 gtagcttcaa cgatttggta tcatcgcaaa aaagtattgt cagcagacca agtacttgaa
     6001 aatatcaagt catcttttct aaaagaaggt gcaatcgaag gttcttggat ctcttttgaa
     6061 aaggaaccta ttaggaaatt cgccgttcgg tcgagtgctt atcggggagg tctttctcga
     6121 atagaagatg gcgaaactgt ttattatgaa tttttagcag atgctcatac aggaaccgta
     6181 ttagaaattt caagaaaaaa aggtgcaaca gattaatacg tcaaaaaagt gagagaacga
     6241 atacgcaccc actattcaaa agagaggtat catgtccttc ttttgtagtg ggtggttttt
     6301 tagatagagc tttccaaaca tatattccag tattttcttt ttaaataaat gatatttagc
     6361 gggaaaaact agcatttcct ttgattatga ggtattttta atttagcttt tgttttctat
     6421 agttaaggag agtgaaaaat ggaacaacaa gaaaaaattg ctgttctgat tccttgttac
     6481 aatgaggaag caacaatttc aacagtcatt gcagacttta aacgagaatt accagaagca
     6541 gatatttatg tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggt
     6601 ggagcaatcg taaaaaaaga acctcgtcaa ggaaaaggaa atgtgatccg ccaaatgttc
     6661 tttgatatcg atgccgacta ttatttaatg gtagatggcg atgatactta tcctgcagaa
     6721 gctgtacatg gtttgctcga gaaattacgt tctggtgaag cagatatggt cattggtgat
     6781 cgcctatcaa atggaaccta ctttgatgag aataagagac ctttccacga ttttggaaat
     6841 aaccttgtta gaaatacaat tactagaatg tataaaacaa aaattcttga tgtcatgacc
     6901 ggctatcgag gattcaatcg tatctttgtc aaaagttttc ctattatgag ttctggtttt
     6961 caaatcgaaa cggaattgac gatccatgct ttagataaaa aattcaagtt cgttgaaatc
     7021 ccaatcgatt atcgtgatcg tcctgaaggc agcgaatcaa aattgaatac attctctgac
     7081 ggattcaaag tgatcatgat gattattaaa atgtttaaag attataagcc tttgcttttc
     7141 tttgggattt ggaccatctt cttcttttta tttggattgg tcacggggat tcctgtgatt
     7201 cgcgaatata tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga
     7261 ttgatgactt tagcacttct ttcattagta acagggttga ttttggatac agtcgtcacg
     7321 aacgctaaga aagaatatga attgaatcta tatcatgttt atcatgaata ccacagtaga
     7381 aagtaaaaaa aggatcaaga ctgcaaaaat aatttgcaat tcttgatcct ctttttaatt
     7441 aattatttcg ccaaacgata gatggcatct gcatagattg ctgttgctct gaacaagtca
     7501 tcaagactca taaattcgtt tgcttgatgc attgtatcag tatatcctgg gaacatcgct
     7561 ccataagcaa cgccacgttt caacaaacgt ccgtaagttc cgccaccgat gatttgttcg
     7621 tagccttttt ctccagtgtg gtcttcatag acttgcaata atgtagccac caatggatca
     7681 tccattggta cgtaatgtgg ttccattacg cgagcacctc gagtaactgt tgcgccttct
     7741 tgccccactg tttgttcgat gtcactttgc aatccgtcta ctgttacacc ttttggaaaa
     7801 cggaaattca tattgatgaa gttcgcttcc tctgtaccat tttcttcaaa agcaaatagt
     7861 ccagcattca ttgtcaattt gcccattttt tcatcttcat aagcgactcc tagtttttct
     7921 ccataaaaat cttcatggac ataagcagct gctacatgga taaattgttt agcagatcct
     7981 aggaattcat agttatctaa gaacaatgcc aagaaagaac ctgcattgat accagattgt
     8041 gggcttgcac catgtgcgcc tttaccaact aattcgattt ttacatacgt attgtccgct
     8101 tcaatcgttc cgcttactgg atttgcttcg atgaattggt aaaatgcttc ttccatcgcg
     8161 atagctgcat ctgcacttgg tacttgaagt gctgcagttg ctgttcctgg caccatattt
     8221 tcgcgaagtc cggaaacaaa agatttcaag acataatctc ctgcattgtc gcctttgaag
     8281 cgtaaagcta aagaaacatt tcctttttca ccattgatga ttgggaattc agcatctggt
     8341 gagaaaccaa aatctggttc ttcttcatgt tcgaaataat aagccatatc gccccagccg
     8401 ctttcttcat cgctgccgac tacgaagcga actttttttg atagtttcaa gtcaagttct
     8461 ttaatgatct tcatcgcata gtaagctgcc atacttggac ctttgtcatc gcttgatccg
     8521 cgtgcaaaga tctttccatc cttgattact ggttcataag gatctgtgtc ccagccatct
     8581 cctgcaggaa cgacatccat gtgtccaaat atccctagcg tttcgtctcc ttcgccaaga
     8641 tcaatatgcc ctgcatagtt atcgacattt tttactttaa atccatcacg ttcgccataa
     8701 gctaacatat gtttcaatgc gtcgcgaggt ccaggtccaa atggtgcatc tggtgttact
     8761 tttgagtcat cgcgctcgct gttgacacgt aaaagatttt tcaagtcttc aagcaagtct
     8821 tctttacgtg cttctacttc tttttgccaa tcaattgtca taaagttaat cctccactta
     8881 tttaagtctt cgtgttcatg ataccacaaa ttacagaaaa aaatagttca aaaaatctga
     8941 caattccttt tcttatctgt ttttctctaa aaaaaatata cgctataatg agtaaaagga
     9001 ggtttttaga tgctgataaa cgaacaacta gtgaaaagag tcatcacacc tcgtccaaaa
     9061 atttcacata aaggaacatt tggacgtatc gttttgattg gcggcaatga acaatatggc
     9121 ggagcaatca ttatgagtgc tttagctgcc gtacacagcg gagcgggtct cacgactgta
     9181 gtaactgatg aaaaaaatcg gagtgctctt cacagtcatc tgccagaagc aatgattgta
     9241 gtttggacgg atactgacca gtttctttcc gtaataaagc aagcagatgt actcttgatt
     9301 ggtcccggat tgggtacttc ttctaagagt ttggaacgtt tcaaacaagt actagaaaac
     9361 caatcaaaag aacaatggct ggtgattgat ggctctgctt taagtttact tgctgctaat
     9421 catcttttgc taccctatcc cgaacaaact gtacttacac ctcatcaaat ggagtggcaa
     9481 cggttgagtt ctctaccgat acaagaacaa cgggataaag aaaacaaaaa gaagcaacag
     9541 gaaatcggtg ctgtgctcgt tttgaaaagt catgagacaa aaatttatgg tcaccagcac
     9601 ttctacacca atcctttagg ttctgctgct atggcaacgg gcggcatggg tgacacttta
     9661 gctgggatga tcacaggatt tttagcccag tttcctcaaa atgaccaaac gattgcagca
     9721 gctatttatc ttcatagttt cattggagac cagctggcaa aagaacaata tattgttttg
     9781 ccgacacaaa tcagcgaagc acttccgaaa tggatgaatt atttttgtaa aaaatcaatg
     9841 gaaaacgagg catgagtcat ggagaaattt acaggttatg tgcgagaacc tttttattat
     9901 gaaacagatc aaatggggat tattcatcac agcaattata tccgctggtt tgaagaggct
     9961 cgggtagcct tattggtaca tctagggttt ccctataaaa aaatcgaaga atctgggatc
    10021 atcatccccg ttctggaagt aaattgcagc tataaagaaa tgatccatta cggagaaaaa
    10081 gtgagcatcc atccaactat ccaaaagtat acggggacac ggttggattt tagctacgaa
    10141 attgtgggaa ctgaagacaa aaaattaaaa actactggca gtagtaaaca ctgtttttta
    10201 ctttctgatt cgcaacgatt agtgaagctg agcaaagtca atccggagtt agatcagtta
    10261 ttccaagcgt acgcttcttt ggatgatgta aattcttaat aaagaccgtc taagcatttt
    10321 tcttgtattg tgtcgagtag ttcgtataat gaaaagaaaa aagcaggtga tccccatgta
    10381 tgtcgttaaa gtaatgcacg gttacatcga taaaactggc tgtcgcactc gggaaaaaaa
    10441 cttagacaat ctgctcattt tcaaagacaa aaaagaatcc gaagcttttg ctaaaagaat
    10501 cggcggtcgt gtcaaaccga tccaagaggt acgaccagat tgattcaata tttttctttt
    10561 cgaggctcta ataggcatgc ctatcagggc tttttttgca gactgtgttt ttatgcccac
    10621 acgatttgta agcgatttat gttataatag attatatctg catagaaagg aaggttaaat
    10681 ggttatcaat aatttgcaac agcaagctta tgaatctata cgtaaacaaa tcatttattc
    10741 cgagttagca cctggaagta aaatctccga taaagtgtta gaagaacaac tagctatcgg
    10801 gcgtacaccg attcgagaag cactgatcca actgcgcaat caagagcttg taaaaaccat
    10861 tccacagtcc ggcacttatg tttcaaaaat cgatatccga tctgcttcat tagcccgtta
    10921 tacgcgggaa aagttggaaa aaccgatcct gcaagaatgc agtgcgaaaa tgaccaagca
    10981 agatcaagct aaactagaaa aaatactaga acaaaccgat caggcagtaa tcgctcaaaa
    11041 taaaaaagct ttttttgatt tggatactga ttttcaccga acttgttatg aaattgctgg
    11101 aaaacgagaa atttgggatt ggctagaaag ttatagtacg cgtttcagat ggttacgttt
    11161 gacgatttca gaattagact ggggtcgagt attagatgaa catcagacaa tgcttcaatc
    11221 aatgattgat cataactttg atgaagtcgg atttctttgc acgatgcatt tacatatgat
    11281 tatcgaggag caagaatacg tgattcataa ttaccccgat tattttgaag atattcagga
    11341 cagacccatc aaataagagg ttttggaatc agcgttttga cctgagtatt agagaaagaa
    11401 acggcaaaat agttcctcat attttgccgt tttttttgag ctaataccga gggtcagctg
    11461 attgcacacc gtttattgag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    11521 gccaagtaaa aatagcttct tatgttttta acttggctgg acttattacc gcagatgcaa
    11581 gcttttgaac accgttttcc aagaggtcgt gagcctgccg ttcatctccg agtcacaagg
    11641 aacaatttca aaaaacagct tctcatgttt gttgaatatt ttgacttgtg accgaggagt
    11701 tgacaggaga acatcgttta ttcggaaaaa aaggactgtg acacgacttt tgtcacagtc
    11761 tctttatttg taatctaaat catactccgt acatcttttt tctatttttt ttatttcttc
    11821 taagatccct tggctacgaa tttcattata tggccaaagt aagactgcct ctaatacata
    11881 aggcgaaaaa tcgctttgat tcatctcttt tgaaaaactc atcgctgcta tctcgctcaa
    11941 tgcacgaatg tttgattgat gtgtataaaa aggcaaccgc cacagcacaa ttttttcgct
    12001 ttgcgtataa attcccttaa cagaagcttc gatgtggtga aatagttcat gtccaataat
    12061 caaagcttgc gctgtttctt tagagatcga agctagtcct gaacatttta atttttctaa
    12121 cggctccgtc atcaatcgaa tcccttcatc ttctgcataa gttgcaaaag tgattcgttc
    12181 gtttatttgc tctgattcac aggaaacgat tggaacagcc atttgttcac aaatttctcc
    12241 tacatcggca aaagggtact tttttttgat gctacatgcc gtttcgatac cacaatcgat
    12301 tgattgttgt ataagaattt tctgcttgga caaaggaatc tttttccaaa ataaatctcg
    12361 ccgaaactgg tagaatcccc aaatttcttc ggggatctgt tgtaattcgt ttagtaactc
    12421 ttctaactta tgtttgtttg atatttccgg caacgacgat cacttcttcc tccggttcca
    12481 tagtagatac ttccagatcc atcaaaagct gtagttgttc cagatccgtc gctgtaaaat
    12541 cagagactct tgctcctaag cagagataaa attctggtcg ggcaatcaat tcagtttgat
    12601 aaactgccat atcttcccaa agttgttcga cagctgtcat ataattggct gcagttgttt
    12661 tcatacatga agcatgtcca cgttggacct tgataaatcc tttttgatcg atgatccgaa
    12721 gacttccagc attttttgaa cgattctgtt cgccgcatac ataaaagaaa ggtgtatttt
    12781 ccaataagca aacttcattt ttatttaatc gcatatcctc tgcagcaagc tctagtgctt
    12841 cttctgttgt gatctccttg gttagatcgg ttgctttcac ttccgtagaa ccagtagcga
    12901 tagccgttac ttttgaagtc tgtggatcga tttctacgtg gacttcgatc gattctggtg
    12961 tagcgcctga ttcaatcgct ttattcatcg cttcattttt caacgatcgt atatcttctt
    13021 tacttggcga agggatgata cgttctacaa catctcggac cattgcaagt gctacaccaa
    13081 tagaagaaat cacttcggcg ttttcaggaa tcgaatattt cacacccatt ttattactaa
    13141 agtaagtaat caatgaggca gcgccgcccc ctacacctac aagagaaatc tgatctttct
    13201 ccaagtgata cttgtctgcc aattctaaaa tcaccggttc aatcttagca taggattttt
    13261 ccatgatctg ttctgcgatg tcttcaaccg tcgtatgaca ataatcagct aatgcttgga
    13321 tggcttttcg tgcagatggg acatttccat aagaaaaatg ttcttcttgc actaatccaa
    13381 ggacattggc tgcacatgta tttgtaatag tcacttcttc tccgtcttcc atcactacct
    13441 tgacgtaatc agcagggtct ccttctttag gagagaaaaa ttcaacctta ggacctttga
    13501 ttttttctgt ctccgtaaat actgcataat ctagaccggc aatatgtgca gaacgtggac
    13561 cgacatcgat gattcctgac tgattagccc gtaccatcga accgcctgcc acacctagaa
    13621 cccgaacatc taatgaagag atatatgttg ggtggccacc tacaattgaa taatcgattg
    13681 ctggacgtcc atttttaatg accccgatat tggttgttgt tccgcctact tcaaaataaa
    13741 caccattaga cgctctcaag tacatcaaag agcccatgac tgatgctgct ggaccggaaa
    13801 gcatggttaa aacaggtcgt tttttcattt cattgatttc cattactccg ccgtctccgc
    13861 gcataatcat caaagaaaca ttgacccctg cttctcgtac ggaatcttct gtagaggttg
    13921 ccgtatctag catttttggt aaaatagatg cattgattgc tgctgtccga gttcttcttg
    13981 tcaatccata taattttgtg atgtcagaag ccatcgttgt agggatcgat tttaccgatg
    14041 ctgcttcata aacaactcgt tctggttccc cattatctac tccaaatgcc atagaagaaa
    14101 ccaagacctc cgctctttct ctttctaaag aagaaatcgt ttctgaaaca cggtctacat
    14161 ttaagtgttt gacaggtaag aatgcattga caatctctat ttttttctta ttccctaaat
    14221 cgatatcgtt caatctagtt tgtcgttttg ctaaaaaacc ttctaatcca ccttttgcca
    14281 tcccgattac gccgactttt gctacgtctc cttcaatcag tgcattagtc gcttgtgtgg
    14341 tagaatgtgc aacaaacaca acatcttctg gactgatatt attttccagc aaacaatttt
    14401 gaaaagactg aacgacacct gcggctactc cacggacatc atcgtgggtt gttttcactg
    14461 aagattttcc gatgatttcg tgcgtcgcat tatcgattgc gactgctttc gtatgtgtac
    14521 cgccaacatc aattcccatt cgcacaaaac gtttcatttt cttttcctcc attcatctaa
    14581 tcgtttttat cgacgttttg tttactaggc gttcccgtac aataagaacg tgatcaggca
    14641 taagatcgct ccgatgatcc aaccagttgg gatggatttc ttcatatatt ctcgagaagt
    14701 tacttttgta taacctaatc cccaagcaac ccaagattgt gtaatatcta aatgctgagg
    14761 agcaatcgtt gtgattgcaa ataacgggaa caaaaaggct ggcgacatcg ttggattaac
    14821 agctaatacg acagctaaaa tagctgagcc tgaaccgact agattcattg gaccgcggaa
    14881 aaatcctagt ggtgtcaata tagcaaatac gattgccaag actaatggtg attgcggtat
    14941 ccaatctcca aaaatagctg aaaaataagg agaagcatac gcagctgaat tattgaacat
    15001 cgctaaggtc atcaagaaac caaccattgg cgcaacatct actgcgccat ctgtaaataa
    15061 tttcgcaaac tgacgacaga tttctttata acctccgcgc aattttccag taaacagcaa
    15121 tgcccagata cctgctagaa taaaacctaa gatgattgga agatctaaaa gaacgacccc
    15181 taaaactggt aataatacgg ctaaccatga gatcattggt gcgtcgccac cttcagctgg
    15241 tacgtttgct gccattgcat agcgtttctt tttgttcatt gatatattag cgaccgttag
    15301 taccacaact aagcttacaa tcatcccaat cataccaatt tggaaatagt cattataagt
    15361 atagctttca gccgctggat tgaaaccagc ataaatcgtt tgatattgtt tgaaattgac
    15421 gatattagca aagattccag ccataatcga gcccataaat gagaacagtg caaccggtac
    15481 tgaaatacct aatgacatca agattggcaa cacaataact gcaatagaaa tcactggtcc
    15541 gattcctgtc atcgacatga atataacagc ggttacaata catagtaagc tcatcgtgat
    15601 tcttggcttg tcccctccaa gttcaactac ctttcgaatc agggtagctg cgattccgct
    15661 atctactaga acacgcccga aaaaggcacc aaagaagaca tttaccaaga tcgatttggc
    15721 gtactcagct ggccccgtcg tatagatatg cgtcaacaca tcaattactc cttgcccttc
    15781 catcgccggg tttggtgaaa acgtgtttcc gactagcgct aatcccatcc atacagtagc
    15841 catgatggca aagccgacca tcaagttgta gccttttaca cagtaccaga ccatgaaaaa
    15901 gaatgagata actaacaaaa taccaataat aatatcactc atgttattcc tccgtccatt
    15961 ttgtttttct gataagcaaa ctaatatatc agtcaaactc attctaactt atgtagttag
    16021 cgctttcatt aaagggtgac cataaatgtt cagttttcaa ttaaaaaacc ttaattatgc
    16081 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
    16141 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
    16201 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
    16261 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
    16321 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
    16381 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
    16441 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
    16501 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
    16561 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
    16621 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
    16681 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
    16741 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
    16801 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
    16861 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
    16921 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
    16981 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
    17041 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
    17101 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
    17161 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
    17221 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
    17281 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
    17341 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
    17401 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
    17461 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
    17521 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
    17581 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
    17641 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
    17701 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
    17761 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
    17821 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
    17881 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
    17941 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
    18001 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
    18061 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
    18121 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
    18181 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
    18241 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
    18301 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
    18361 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
    18421 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
    18481 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
    18541 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
    18601 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
    18661 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
    18721 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
    18781 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
    18841 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
    18901 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
    18961 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
    19021 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
    19081 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
    19141 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
    19201 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
    19261 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
    19321 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
    19381 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
    19441 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
    19501 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
    19561 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
    19621 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
    19681 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
    19741 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
    19801 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
    19861 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
    19921 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
    19981 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
    20041 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
    20101 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
    20161 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
    20221 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
    20281 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
    20341 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
    20401 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
    20461 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
    20521 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
    20581 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
    20641 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
    20701 actattagaa aatgtccagc tttcggaaaa aatcgcccat ggtaatccta gtggaatcga
    20761 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
    20821 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
    20881 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
    20941 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
    21001 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
    21061 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
    21121 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
    21181 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
    21241 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
    21301 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
    21361 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
    21421 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
    21481 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
    21541 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
    21601 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
    21661 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
    21721 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
    21781 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
    21841 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
    21901 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
    21961 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
    22021 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
    22081 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
    22141 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
    22201 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
    22261 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
    22321 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
    22381 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
    22441 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
    22501 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
    22561 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
    22621 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
    22681 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
    22741 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
    22801 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
    22861 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
    22921 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
    22981 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
    23041 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
    23101 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
    23161 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
    23221 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
    23281 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
    23341 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
    23401 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
    23461 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
    23521 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
    23581 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
    23641 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
    23701 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
    23761 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
    23821 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
    23881 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
    23941 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
    24001 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
    24061 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
    24121 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
    24181 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
    24241 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
    24301 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
    24361 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    24421 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    24481 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
    24541 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
    24601 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
    24661 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
    24721 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
    24781 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
    24841 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
    24901 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
    24961 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
    25021 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
    25081 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
    25141 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
    25201 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
    25261 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
    25321 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
    25381 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
    25441 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
    25501 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
    25561 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
    25621 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
    25681 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
    25741 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
    25801 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
    25861 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
    25921 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
    25981 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
    26041 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    26101 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    26161 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    26221 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    26281 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    26341 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    26401 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    26461 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    26521 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    26581 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    26641 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    26701 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    26761 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    26821 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    26881 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    26941 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    27001 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    27061 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    27121 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    27181 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    27241 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    27301 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    27361 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    27421 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    27481 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    27541 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    27601 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    27661 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    27721 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    27781 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    27841 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    27901 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    27961 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    28021 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    28081 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    28141 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    28201 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    28261 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    28321 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    28381 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    28441 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    28501 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    28561 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    28621 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    28681 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    28741 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    28801 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    28861 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    28921 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    28981 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    29041 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    29101 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    29161 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    29221 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    29281 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    29341 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    29401 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    29461 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    29521 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    29581 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    29641 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    29701 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    29761 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    29821 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    29881 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    29941 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    30001 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    30061 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    30121 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    30181 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    30241 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    30301 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    30361 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    30421 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    30481 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    30541 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    30601 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    30661 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    30721 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    30781 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    30841 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    30901 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    30961 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    31021 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    31081 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    31141 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    31201 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    31261 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    31321 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    31381 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    31441 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    31501 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    31561 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    31621 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    31681 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    31741 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    31801 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    31861 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    31921 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    31981 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    32041 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    32101 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    32161 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    32221 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    32281 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    32341 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    32401 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    32461 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    32521 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    32581 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    32641 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    32701 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    32761 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    32821 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    32881 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    32941 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    33001 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    33061 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    33121 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    33181 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    33241 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    33301 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    33361 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    33421 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    33481 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    33541 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    33601 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    33661 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    33721 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    33781 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    33841 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    33901 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    33961 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    34021 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    34081 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    34141 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    34201 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    34261 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    34321 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    34381 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    34441 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    34501 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    34561 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    34621 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    34681 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    34741 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    34801 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    34861 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    34921 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    34981 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    35041 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    35101 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    35161 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    35221 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    35281 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    35341 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    35401 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    35461 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    35521 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    35581 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    35641 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    35701 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    35761 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    35821 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    35881 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    35941 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    36001 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    36061 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    36121 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    36181 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    36241 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    36301 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    36361 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    36421 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    36481 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    36541 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    36601 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    36661 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    36721 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    36781 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    36841 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    36901 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    36961 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    37021 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    37081 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    37141 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    37201 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    37261 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    37321 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    37381 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    37441 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    37501 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    37561 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    37621 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    37681 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    37741 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    37801 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    37861 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    37921 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    37981 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    38041 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    38101 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    38161 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    38221 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    38281 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    38341 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    38401 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    38461 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    38521 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    38581 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    38641 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    38701 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    38761 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    38821 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    38881 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    38941 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    39001 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    39061 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    39121 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    39181 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    39241 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    39301 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    39361 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    39421 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    39481 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    39541 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    39601 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    39661 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    39721 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    39781 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    39841 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    39901 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    39961 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    40021 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    40081 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    40141 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    40201 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    40261 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    40321 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    40381 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    40441 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    40501 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    40561 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    40621 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    40681 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    40741 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    40801 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    40861 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    40921 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    40981 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    41041 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    41101 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    41161 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    41221 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    41281 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    41341 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    41401 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    41461 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    41521 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    41581 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    41641 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    41701 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    41761 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    41821 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    41881 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    41941 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    42001 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    42061 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    42121 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    42181 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    42241 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    42301 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    42361 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    42421 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    42481 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    42541 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    42601 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    42661 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    42721 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    42781 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    42841 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    42901 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    42961 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    43021 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    43081 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    43141 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    43201 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    43261 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    43321 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    43381 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    43441 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    43501 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    43561 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    43621 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    43681 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    43741 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    43801 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    43861 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    43921 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    43981 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    44041 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    44101 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    44161 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    44221 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    44281 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    44341 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    44401 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    44461 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    44521 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    44581 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    44641 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    44701 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    44761 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    44821 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    44881 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    44941 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    45001 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    45061 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    45121 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    45181 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    45241 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    45301 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    45361 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    45421 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    45481 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    45541 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    45601 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    45661 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    45721 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    45781 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    45841 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    45901 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    45961 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    46021 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    46081 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    46141 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    46201 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    46261 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    46321 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    46381 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    46441 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    46501 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    46561 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    46621 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    46681 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    46741 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    46801 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    46861 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    46921 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    46981 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    47041 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    47101 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    47161 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    47221 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    47281 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    47341 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    47401 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    47461 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    47521 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    47581 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    47641 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    47701 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    47761 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    47821 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    47881 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    47941 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    48001 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    48061 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    48121 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    48181 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    48241 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    48301 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    48361 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    48421 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    48481 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    48541 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    48601 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    48661 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    48721 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    48781 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    48841 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    48901 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    48961 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    49021 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    49081 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    49141 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    49201 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    49261 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    49321 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    49381 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    49441 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    49501 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    49561 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    49621 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    49681 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    49741 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    49801 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    49861 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    49921 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    49981 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    50041 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    50101 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    50161 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    50221 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    50281 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    50341 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    50401 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    50461 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    50521 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    50581 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    50641 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    50701 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    50761 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    50821 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    50881 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    50941 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    51001 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    51061 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    51121 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    51181 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    51241 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    51301 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    51361 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    51421 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    51481 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    51541 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    51601 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    51661 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    51721 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    51781 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    51841 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    51901 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    51961 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    52021 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    52081 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    52141 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    52201 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    52261 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    52321 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    52381 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    52441 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    52501 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    52561 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    52621 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    52681 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    52741 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    52801 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    52861 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    52921 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    52981 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    53041 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    53101 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    53161 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    53221 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    53281 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    53341 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    53401 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    53461 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    53521 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    53581 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    53641 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    53701 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    53761 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    53821 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    53881 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    53941 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    54001 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    54061 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    54121 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    54181 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    54241 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    54301 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    54361 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    54421 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    54481 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    54541 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    54601 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    54661 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    54721 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    54781 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    54841 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    54901 tccttatggg caaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    54961 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    55021 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    55081 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    55141 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    55201 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    55261 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    55321 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    55381 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    55441 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    55501 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    55561 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    55621 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    55681 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    55741 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    55801 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    55861 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    55921 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    55981 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    56041 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    56101 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    56161 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    56221 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    56281 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    56341 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    56401 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    56461 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    56521 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    56581 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    56641 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    56701 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    56761 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    56821 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    56881 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    56941 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    57001 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    57061 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    57121 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    57181 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    57241 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    57301 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    57361 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    57421 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    57481 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    57541 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    57601 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    57661 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    57721 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    57781 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    57841 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    57901 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    57961 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    58021 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    58081 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    58141 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    58201 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    58261 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    58321 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    58381 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    58441 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    58501 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    58561 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    58621 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    58681 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    58741 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    58801 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    58861 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    58921 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    58981 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    59041 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    59101 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    59161 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    59221 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    59281 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    59341 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    59401 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    59461 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    59521 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    59581 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    59641 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    59701 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    59761 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    59821 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    59881 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    59941 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    60001 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    60061 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    60121 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    60181 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    60241 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    60301 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    60361 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    60421 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    60481 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    60541 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    60601 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    60661 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    60721 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    60781 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    60841 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    60901 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    60961 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    61021 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    61081 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    61141 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    61201 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    61261 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    61321 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    61381 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    61441 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    61501 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    61561 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    61621 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    61681 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    61741 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    61801 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    61861 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    61921 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    61981 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    62041 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    62101 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    62161 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    62221 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    62281 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    62341 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    62401 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    62461 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    62521 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    62581 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    62641 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    62701 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    62761 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    62821 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    62881 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    62941 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    63001 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    63061 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    63121 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    63181 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    63241 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    63301 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    63361 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    63421 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    63481 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    63541 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    63601 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    63661 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    63721 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    63781 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    63841 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    63901 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    63961 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    64021 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    64081 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    64141 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    64201 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    64261 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    64321 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    64381 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    64441 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    64501 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    64561 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    64621 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    64681 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    64741 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    64801 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    64861 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    64921 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    64981 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    65041 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    65101 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    65161 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    65221 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    65281 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    65341 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    65401 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    65461 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    65521 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    65581 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    65641 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    65701 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    65761 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    65821 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    65881 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    65941 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    66001 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    66061 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    66121 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    66181 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    66241 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    66301 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    66361 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    66421 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    66481 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    66541 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    66601 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    66661 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    66721 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    66781 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    66841 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    66901 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    66961 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    67021 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    67081 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    67141 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    67201 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    67261 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    67321 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    67381 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    67441 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    67501 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    67561 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    67621 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    67681 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    67741 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    67801 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    67861 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    67921 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    67981 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    68041 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    68101 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    68161 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    68221 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    68281 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    68341 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    68401 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    68461 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    68521 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    68581 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    68641 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    68701 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    68761 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    68821 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    68881 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    68941 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    69001 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    69061 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    69121 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    69181 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    69241 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    69301 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    69361 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    69421 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    69481 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    69541 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    69601 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    69661 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    69721 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    69781 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    69841 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    69901 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    69961 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    70021 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    70081 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    70141 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    70201 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    70261 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    70321 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    70381 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    70441 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    70501 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    70561 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    70621 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    70681 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    70741 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    70801 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    70861 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    70921 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    70981 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    71041 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    71101 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    71161 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    71221 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    71281 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    71341 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    71401 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    71461 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    71521 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    71581 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    71641 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    71701 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    71761 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    71821 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    71881 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    71941 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    72001 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    72061 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    72121 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    72181 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    72241 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    72301 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    72361 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    72421 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    72481 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    72541 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    72601 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    72661 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    72721 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    72781 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    72841 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    72901 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    72961 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    73021 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    73081 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    73141 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    73201 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    73261 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    73321 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    73381 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    73441 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    73501 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    73561 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    73621 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    73681 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    73741 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    73801 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    73861 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    73921 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    73981 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    74041 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    74101 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    74161 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    74221 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    74281 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    74341 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    74401 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    74461 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    74521 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    74581 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    74641 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    74701 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    74761 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    74821 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    74881 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    74941 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    75001 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    75061 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    75121 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    75181 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    75241 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    75301 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    75361 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    75421 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    75481 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    75541 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    75601 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    75661 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    75721 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    75781 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    75841 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    75901 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    75961 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    76021 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    76081 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    76141 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    76201 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    76261 aaactgcctc ct
//
LOCUS       DAYYHS010000003        64888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000003 DAYYHS010000000
VERSION     DAYYHS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.003"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1421)
                     /locus_tag="ACHA6A_000164"
     CDS             complement(<1..1421)
                     /locus_tag="ACHA6A_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8968405.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="ACHA6A_000165"
     CDS             1673..2854
                     /locus_tag="ACHA6A_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8968406.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHA6A_000166"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="ACHA6A_000166"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8968407.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="ACHA6A_000167"
     CDS             complement(4993..7704)
                     /locus_tag="ACHA6A_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8968408.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="ACHA6A_000168"
     CDS             complement(7714..8415)
                     /locus_tag="ACHA6A_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968409.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="ACHA6A_000169"
     CDS             8601..9200
                     /locus_tag="ACHA6A_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8968410.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="ACHA6A_000170"
     CDS             9281..10294
                     /locus_tag="ACHA6A_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8968411.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHA6A_000171"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="ACHA6A_000171"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8968412.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHA6A_000172"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="ACHA6A_000172"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8968413.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="ACHA6A_000173"
     CDS             11742..13115
                     /locus_tag="ACHA6A_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8968414.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="ACHA6A_000174"
     CDS             13472..15616
                     /locus_tag="ACHA6A_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8968415.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="ACHA6A_000175"
     CDS             complement(15668..16708)
                     /locus_tag="ACHA6A_000175"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8968416.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="ACHA6A_000176"
     CDS             complement(16985..18787)
                     /locus_tag="ACHA6A_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8968417.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="ACHA6A_000177"
     CDS             complement(18801..19553)
                     /locus_tag="ACHA6A_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968418.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="ACHA6A_000178"
     CDS             complement(19562..19924)
                     /locus_tag="ACHA6A_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8968419.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="ACHA6A_000179"
     CDS             complement(20100..20972)
                     /locus_tag="ACHA6A_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8968420.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="ACHA6A_000180"
     CDS             complement(21023..21511)
                     /locus_tag="ACHA6A_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8968421.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22440)
                     /locus_tag="ACHA6A_000181"
     CDS             complement(21649..22440)
                     /locus_tag="ACHA6A_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8968422.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22495..23103)
                     /locus_tag="ACHA6A_000182"
     CDS             complement(22495..23103)
                     /locus_tag="ACHA6A_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8968423.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="ACHA6A_000183"
     CDS             complement(23096..23413)
                     /locus_tag="ACHA6A_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8968424.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="ACHA6A_000184"
     CDS             complement(23569..24297)
                     /locus_tag="ACHA6A_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8968425.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="ACHA6A_000185"
     CDS             24456..25337
                     /locus_tag="ACHA6A_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8968426.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26617)
                     /locus_tag="ACHA6A_000186"
     CDS             complement(25574..26617)
                     /locus_tag="ACHA6A_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8968427.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26620..27288)
                     /locus_tag="ACHA6A_000187"
     CDS             complement(26620..27288)
                     /locus_tag="ACHA6A_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8968428.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27430..29766
                     /locus_tag="ACHA6A_000188"
     CDS             27430..29766
                     /locus_tag="ACHA6A_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8968429.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29881..30192)
                     /locus_tag="ACHA6A_000189"
     CDS             complement(29881..30192)
                     /locus_tag="ACHA6A_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968430.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30373..31848
                     /locus_tag="ACHA6A_000190"
     CDS             30373..31848
                     /locus_tag="ACHA6A_000190"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8968431.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31895..32479
                     /locus_tag="ACHA6A_000191"
     CDS             31895..32479
                     /locus_tag="ACHA6A_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8968432.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32481..32864
                     /locus_tag="ACHA6A_000192"
     CDS             32481..32864
                     /locus_tag="ACHA6A_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8968433.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33181..33465)
                     /locus_tag="ACHA6A_000193"
     CDS             complement(33181..33465)
                     /locus_tag="ACHA6A_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8968434.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33817..34527)
                     /locus_tag="ACHA6A_000194"
     CDS             complement(33817..34527)
                     /locus_tag="ACHA6A_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8968435.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34524..35996)
                     /locus_tag="ACHA6A_000195"
     CDS             complement(34524..35996)
                     /locus_tag="ACHA6A_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8968436.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36134..36607
                     /locus_tag="ACHA6A_000196"
     CDS             36134..36607
                     /locus_tag="ACHA6A_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8968437.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36760..37311)
                     /locus_tag="ACHA6A_000197"
     CDS             complement(36760..37311)
                     /locus_tag="ACHA6A_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8968438.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37416..37946
                     /locus_tag="ACHA6A_000198"
     CDS             37416..37946
                     /locus_tag="ACHA6A_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8968439.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38429..38872)
                     /locus_tag="ACHA6A_000199"
     CDS             complement(38429..38872)
                     /locus_tag="ACHA6A_000199"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8968440.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38888..40048)
                     /locus_tag="ACHA6A_000200"
     CDS             complement(38888..40048)
                     /locus_tag="ACHA6A_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8968441.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40555..42255
                     /locus_tag="ACHA6A_000201"
     CDS             40555..42255
                     /locus_tag="ACHA6A_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968442.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42357..43190)
                     /locus_tag="ACHA6A_000202"
     CDS             complement(42357..43190)
                     /locus_tag="ACHA6A_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8968443.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43494..45659)
                     /locus_tag="ACHA6A_000203"
     CDS             complement(43494..45659)
                     /locus_tag="ACHA6A_000203"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8968444.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45919..48213
                     /locus_tag="ACHA6A_000204"
     CDS             45919..48213
                     /locus_tag="ACHA6A_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8968445.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48206..48871
                     /gene="pgmB"
                     /locus_tag="ACHA6A_000205"
     CDS             48206..48871
                     /gene="pgmB"
                     /locus_tag="ACHA6A_000205"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8968446.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48864..49871
                     /locus_tag="ACHA6A_000206"
     CDS             48864..49871
                     /locus_tag="ACHA6A_000206"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8968447.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49893..50912
                     /locus_tag="ACHA6A_000207"
     CDS             49893..50912
                     /locus_tag="ACHA6A_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8968448.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="ACHA6A_000208"
     CDS             complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="ACHA6A_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8968449.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52669..54090)
                     /gene="tyrP"
                     /locus_tag="ACHA6A_000209"
     CDS             complement(52669..54090)
                     /gene="tyrP"
                     /locus_tag="ACHA6A_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8968450.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="ACHA6A_000210"
     CDS             complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="ACHA6A_000210"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8968451.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYIFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56501..57757)
                     /gene="tyrS"
                     /locus_tag="ACHA6A_000211"
     CDS             complement(56501..57757)
                     /gene="tyrS"
                     /locus_tag="ACHA6A_000211"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8968452.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57869..58074)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58337..59638
                     /locus_tag="ACHA6A_000212"
     CDS             58337..59638
                     /locus_tag="ACHA6A_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8968453.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59707..60306)
                     /locus_tag="ACHA6A_000213"
     CDS             complement(59707..60306)
                     /locus_tag="ACHA6A_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8968454.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60560..61441
                     /locus_tag="ACHA6A_000214"
     CDS             60560..61441
                     /locus_tag="ACHA6A_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8968455.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61454..63064
                     /locus_tag="ACHA6A_000215"
     CDS             61454..63064
                     /locus_tag="ACHA6A_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8968456.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63141..63800)
                     /locus_tag="ACHA6A_000216"
     CDS             complement(63141..63800)
                     /locus_tag="ACHA6A_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8968457.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64083..64748)
                     /locus_tag="ACHA6A_000217"
     CDS             complement(64083..64748)
                     /locus_tag="ACHA6A_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8968458.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        19458 a        13217 c        11704 g        20509 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga
    25981 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    26041 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    26101 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    26161 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    26221 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    26281 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    26341 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    26401 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    26461 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    26521 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    26581 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    26641 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    26701 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    26761 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    26821 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    26881 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    26941 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    27001 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    27061 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    27121 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    27181 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    27241 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    27301 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    27361 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    27421 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    27481 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    27541 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    27601 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    27661 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    27721 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    27781 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa
    27841 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    27901 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    27961 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    28021 cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag
    28081 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    28141 agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa
    28201 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    28261 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    28321 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    28381 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    28441 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    28501 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    28561 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    28621 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    28681 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    28741 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    28801 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    28861 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    28921 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    28981 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    29041 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    29101 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    29161 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    29221 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    29281 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    29341 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    29401 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    29461 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    29521 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    29581 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    29641 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    29701 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    29761 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    29821 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    29881 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    29941 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    30001 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    30061 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    30121 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc
    30181 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    30241 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    30301 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    30361 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    30421 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    30481 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    30541 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    30601 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    30661 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    30721 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    30781 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    30841 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    30901 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    30961 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    31021 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    31081 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    31141 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    31201 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    31261 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    31321 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    31381 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    31441 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    31501 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    31561 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    31621 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    31681 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    31741 ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    31801 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    31861 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    31921 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    31981 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    32041 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    32101 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    32161 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    32221 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    32281 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    32341 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    32401 tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa
    32461 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    32521 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    32581 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    32641 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    32701 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    32761 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    32821 agattttaga ttggttggaa aaaatagatt tccatatcaa ataaccagtt tattataaag
    32881 atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    32941 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    33001 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    33061 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    33121 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    33181 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    33241 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    33301 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    33361 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac
    33421 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    33481 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    33541 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    33601 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    33661 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    33721 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    33781 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    33841 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    33901 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    33961 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    34021 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    34081 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    34141 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    34201 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    34261 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    34321 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    34381 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    34441 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    34501 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    34561 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    34621 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    34681 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    34741 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    34801 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    34861 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    34921 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    34981 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    35041 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    35101 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    35161 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    35221 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    35281 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    35341 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    35401 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    35461 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    35521 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    35581 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca
    35641 ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    35701 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    35761 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    35821 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    35881 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    35941 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    36001 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    36061 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    36121 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    36181 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    36241 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    36301 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    36361 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    36421 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    36481 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    36541 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    36601 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    36661 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    36721 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    36781 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    36841 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    36901 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    36961 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    37021 attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc
    37081 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    37141 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    37201 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    37261 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    37321 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    37381 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    37441 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    37501 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    37561 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    37621 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    37681 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    37741 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    37801 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    37861 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    37921 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    37981 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    38041 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    38101 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    38161 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    38221 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    38281 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    38341 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    38401 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    38461 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    38521 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    38581 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    38641 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    38701 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    38761 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    38821 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    38881 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    38941 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    39001 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    39061 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    39121 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    39181 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    39241 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    39301 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    39361 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    39421 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    39481 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    39541 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    39601 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    39661 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    39721 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    39781 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    39841 ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    39901 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    39961 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    40021 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    40081 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    40141 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    40201 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    40261 aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc
    40321 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    40381 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    40441 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    40501 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    40561 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    40621 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    40681 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    40741 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    40801 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    40861 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    40921 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    40981 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    41041 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    41101 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    41161 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    41221 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    41281 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    41341 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    41401 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    41461 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    41521 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    41581 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    41641 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    41701 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    41761 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    41821 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    41881 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    41941 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    42001 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    42061 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa
    42121 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    42181 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    42241 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    42301 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    42361 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    42421 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    42481 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    42541 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    42601 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    42661 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    42721 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    42781 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta
    42841 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    42901 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc
    42961 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag
    43021 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    43081 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    43141 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    43201 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    43261 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    43321 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    43381 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    43441 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    43501 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    43561 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    43621 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    43681 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    43741 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    43801 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    43861 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    43921 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    43981 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    44041 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    44101 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    44161 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    44221 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    44281 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    44341 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    44401 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    44461 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    44521 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    44581 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    44641 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    44701 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    44761 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    44821 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    44881 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    44941 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    45001 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    45061 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    45121 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    45181 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    45241 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    45301 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    45361 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    45421 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    45481 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    45541 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    45601 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    45661 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    45721 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    45781 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    45841 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    45901 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    45961 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    46021 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    46081 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    46141 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    46201 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    46261 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    46321 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    46381 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    46441 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    46501 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    46561 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    46621 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    46681 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    46741 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    46801 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    46861 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    46921 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    46981 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    47041 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    47101 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    47161 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    47221 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    47281 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    47341 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    47401 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    47461 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    47521 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    47581 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    47641 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    47701 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    47761 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    47821 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    47881 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    47941 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    48001 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    48061 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    48121 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    48181 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    48241 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    48301 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    48361 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    48421 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    48481 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    48541 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    48601 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    48661 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    48721 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    48781 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    48841 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    48901 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    48961 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    49021 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    49081 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    49141 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    49201 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    49261 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    49321 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    49381 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    49441 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    49501 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    49561 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    49621 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    49681 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    49741 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    49801 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    49861 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    49921 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    49981 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    50041 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    50101 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    50161 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    50221 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    50281 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
    50341 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    50401 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    50461 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    50521 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    50581 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    50641 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
    50701 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    50761 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    50821 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    50881 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    50941 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    51001 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51061 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    51121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    51181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    51241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    51301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    51361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    51421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    51481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    51541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    51601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    51661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    51721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    51781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    51841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    51901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    51961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    52021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    52081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    52141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    52201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    52261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    52321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    52381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    52441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    52501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    52561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    52621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
    52681 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52741 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52801 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52861 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52921 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    52981 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53041 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53101 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53161 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53221 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53281 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53341 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53401 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53461 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53521 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53581 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53641 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53701 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53761 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53821 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53881 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    53941 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54001 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54061 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54121 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54181 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54241 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54301 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54361 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54421 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54481 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54541 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54601 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54661 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54721 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54781 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54841 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54901 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatatatat
    54961 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55021 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55081 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55141 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55201 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55261 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55321 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55381 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55441 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55501 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55561 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55621 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55681 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55741 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55801 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55861 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55921 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    55981 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56041 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56101 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56161 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56221 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56281 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56341 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56401 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56461 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56521 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56581 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56641 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56701 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56761 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56821 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56881 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    56941 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57001 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57061 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57121 cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57181 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57241 cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57301 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57361 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57421 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57481 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57541 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57601 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57661 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57721 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57781 tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg
    57841 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57901 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    57961 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58021 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58081 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58141 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58201 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58261 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58321 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58381 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58441 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58501 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58561 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58621 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58681 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58741 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58801 tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58861 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58921 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    58981 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59041 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59101 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59161 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59221 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59281 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59341 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59401 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59461 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59521 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59581 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59641 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59701 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59761 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59821 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59881 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    59941 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60001 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60061 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60121 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60181 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60241 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60301 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60361 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60421 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60481 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60541 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60601 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60661 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60721 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60781 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60841 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60901 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    60961 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61021 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61081 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61141 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca ctcgatgaag aaggaacact
    61201 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61261 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61321 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61381 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61441 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61501 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61561 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61621 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61681 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61741 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61801 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61861 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61921 agcggattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    61981 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62041 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62101 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62161 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62221 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62281 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62341 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62401 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62461 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtag tcttgatttg
    62521 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62581 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62641 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62701 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62761 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62821 atggcttcct cgaatcggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62881 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    62941 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63001 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63061 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63121 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63181 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63241 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63301 tgatccttcc ctgtcgcttt agctaagtca agcagataga gagcgactcg ttcttctact
    63361 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63421 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63481 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63541 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63601 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63661 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63721 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63781 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63841 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63901 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    63961 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64021 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64081 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64141 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64201 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64261 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64321 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64381 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64441 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64501 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64561 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64621 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64681 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64741 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64801 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64861 aactgcgatg agcatcaccg cagtttta
//
LOCUS       DAYYHS010000004        62122 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000004 DAYYHS010000000
VERSION     DAYYHS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.004"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHA6A_000218"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHA6A_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8968459.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="ACHA6A_000219"
     CDS             complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="ACHA6A_000219"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8968460.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(2466..4544)
                     /gene="topA"
                     /locus_tag="ACHA6A_000220"
     CDS             complement(2466..4544)
                     /gene="topA"
                     /locus_tag="ACHA6A_000220"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8968461.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="ACHA6A_000221"
     CDS             complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="ACHA6A_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8968462.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(5579..6346)
                     /locus_tag="ACHA6A_000222"
     CDS             complement(5579..6346)
                     /locus_tag="ACHA6A_000222"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8968463.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="ACHA6A_000223"
     CDS             complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="ACHA6A_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8968464.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            7459..7857
                     /locus_tag="ACHA6A_000224"
     CDS             7459..7857
                     /locus_tag="ACHA6A_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8968465.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            8094..8636
                     /locus_tag="ACHA6A_000225"
     CDS             8094..8636
                     /locus_tag="ACHA6A_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8968466.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(8719..9270)
                     /gene="lepB"
                     /locus_tag="ACHA6A_000226"
     CDS             complement(8719..9270)
                     /gene="lepB"
                     /locus_tag="ACHA6A_000226"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8968467.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(9422..9649)
                     /locus_tag="ACHA6A_000227"
     CDS             complement(9422..9649)
                     /locus_tag="ACHA6A_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8968468.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(9646..10164)
                     /gene="msrA"
                     /locus_tag="ACHA6A_000228"
     CDS             complement(9646..10164)
                     /gene="msrA"
                     /locus_tag="ACHA6A_000228"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8968469.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(10184..10897)
                     /locus_tag="ACHA6A_000229"
     CDS             complement(10184..10897)
                     /locus_tag="ACHA6A_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8968470.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(10900..11796)
                     /locus_tag="ACHA6A_000230"
     CDS             complement(10900..11796)
                     /locus_tag="ACHA6A_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8968471.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(11954..12796)
                     /locus_tag="ACHA6A_000231"
     CDS             complement(11954..12796)
                     /locus_tag="ACHA6A_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8968472.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            12928..13581
                     /locus_tag="ACHA6A_000232"
     CDS             12928..13581
                     /locus_tag="ACHA6A_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8968473.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(13644..14171)
                     /locus_tag="ACHA6A_000233"
     CDS             complement(13644..14171)
                     /locus_tag="ACHA6A_000233"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8968474.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(14191..15138)
                     /locus_tag="ACHA6A_000234"
     CDS             complement(14191..15138)
                     /locus_tag="ACHA6A_000234"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8968475.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(15150..17033)
                     /locus_tag="ACHA6A_000235"
     CDS             complement(15150..17033)
                     /locus_tag="ACHA6A_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8968476.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(17196..19274)
                     /locus_tag="ACHA6A_000236"
     CDS             complement(17196..19274)
                     /locus_tag="ACHA6A_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8968477.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(19287..19562)
                     /locus_tag="ACHA6A_000237"
     CDS             complement(19287..19562)
                     /locus_tag="ACHA6A_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968478.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(19924..20379)
                     /locus_tag="ACHA6A_000238"
     CDS             complement(19924..20379)
                     /locus_tag="ACHA6A_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968479.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(20533..21741)
                     /locus_tag="ACHA6A_000239"
     CDS             complement(20533..21741)
                     /locus_tag="ACHA6A_000239"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8968480.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(21853..22185)
                     /locus_tag="ACHA6A_000240"
     CDS             complement(21853..22185)
                     /locus_tag="ACHA6A_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8968481.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            22333..23208
                     /locus_tag="ACHA6A_000241"
     CDS             22333..23208
                     /locus_tag="ACHA6A_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8968482.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(23290..23871)
                     /locus_tag="ACHA6A_000242"
     CDS             complement(23290..23871)
                     /locus_tag="ACHA6A_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8968483.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(23877..25136)
                     /locus_tag="ACHA6A_000243"
     CDS             complement(23877..25136)
                     /locus_tag="ACHA6A_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8968484.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(25138..26184)
                     /locus_tag="ACHA6A_000244"
     CDS             complement(25138..26184)
                     /locus_tag="ACHA6A_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968485.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(26419..26694)
                     /locus_tag="ACHA6A_000245"
     CDS             complement(26419..26694)
                     /locus_tag="ACHA6A_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8968486.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(26937..28247)
                     /gene="der"
                     /locus_tag="ACHA6A_000246"
     CDS             complement(26937..28247)
                     /gene="der"
                     /locus_tag="ACHA6A_000246"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8968487.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(28429..29658)
                     /gene="rpsA"
                     /locus_tag="ACHA6A_000247"
     CDS             complement(28429..29658)
                     /gene="rpsA"
                     /locus_tag="ACHA6A_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8968488.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(29793..30473)
                     /gene="cmk"
                     /locus_tag="ACHA6A_000248"
     CDS             complement(29793..30473)
                     /gene="cmk"
                     /locus_tag="ACHA6A_000248"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8968489.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(30522..31157)
                     /locus_tag="ACHA6A_000249"
     CDS             complement(30522..31157)
                     /locus_tag="ACHA6A_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8968490.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(31223..32626)
                     /locus_tag="ACHA6A_000250"
     CDS             complement(31223..32626)
                     /locus_tag="ACHA6A_000250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8968491.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(32613..33644)
                     /locus_tag="ACHA6A_000251"
     CDS             complement(32613..33644)
                     /locus_tag="ACHA6A_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8968492.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            33675..33896
                     /locus_tag="ACHA6A_000252"
     CDS             33675..33896
                     /locus_tag="ACHA6A_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8968493.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(34061..34798)
                     /locus_tag="ACHA6A_000253"
     CDS             complement(34061..34798)
                     /locus_tag="ACHA6A_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8968494.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(34951..36291)
                     /locus_tag="ACHA6A_000254"
     CDS             complement(34951..36291)
                     /locus_tag="ACHA6A_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8968495.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(36528..38051)
                     /locus_tag="ACHA6A_000255"
     CDS             complement(36528..38051)
                     /locus_tag="ACHA6A_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8968496.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(38429..39040)
                     /locus_tag="ACHA6A_000256"
     CDS             complement(38429..39040)
                     /locus_tag="ACHA6A_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8968497.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(39163..39288)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(39370..40086)
                     /locus_tag="ACHA6A_000257"
     CDS             complement(39370..40086)
                     /locus_tag="ACHA6A_000257"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8968498.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(40092..40661)
                     /gene="scpB"
                     /locus_tag="ACHA6A_000258"
     CDS             complement(40092..40661)
                     /gene="scpB"
                     /locus_tag="ACHA6A_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8968499.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(40645..41415)
                     /locus_tag="ACHA6A_000259"
     CDS             complement(40645..41415)
                     /locus_tag="ACHA6A_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8968500.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(41412..41783)
                     /locus_tag="ACHA6A_000260"
     CDS             complement(41412..41783)
                     /locus_tag="ACHA6A_000260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8968501.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(41802..42689)
                     /gene="xerD"
                     /locus_tag="ACHA6A_000261"
     CDS             complement(41802..42689)
                     /gene="xerD"
                     /locus_tag="ACHA6A_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8968502.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(42785..43258)
                     /locus_tag="ACHA6A_000262"
     CDS             complement(42785..43258)
                     /locus_tag="ACHA6A_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8968503.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(43363..44226)
                     /locus_tag="ACHA6A_000263"
     CDS             complement(43363..44226)
                     /locus_tag="ACHA6A_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8968504.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(44422..46521)
                     /locus_tag="ACHA6A_000264"
     CDS             complement(44422..46521)
                     /locus_tag="ACHA6A_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8968505.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(46741..47850)
                     /gene="rpoD"
                     /locus_tag="ACHA6A_000265"
     CDS             complement(46741..47850)
                     /gene="rpoD"
                     /locus_tag="ACHA6A_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8968506.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(47874..49715)
                     /gene="dnaG"
                     /locus_tag="ACHA6A_000266"
     CDS             complement(47874..49715)
                     /gene="dnaG"
                     /locus_tag="ACHA6A_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8968507.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(49897..52212)
                     /locus_tag="ACHA6A_000267"
     CDS             complement(49897..52212)
                     /locus_tag="ACHA6A_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8968508.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(52397..53257)
                     /locus_tag="ACHA6A_000268"
     CDS             complement(52397..53257)
                     /locus_tag="ACHA6A_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8968509.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(53316..53948)
                     /locus_tag="ACHA6A_000269"
                     /pseudo
     CDS             complement(53316..53948)
                     /locus_tag="ACHA6A_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            54115..54876
                     /locus_tag="ACHA6A_000270"
     CDS             54115..54876
                     /locus_tag="ACHA6A_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8968510.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            54902..57493
                     /gene="mprF"
                     /locus_tag="ACHA6A_000271"
     CDS             54902..57493
                     /gene="mprF"
                     /locus_tag="ACHA6A_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8968511.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEVIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(57681..58868)
                     /gene="tuf"
                     /locus_tag="ACHA6A_000272"
     CDS             complement(57681..58868)
                     /gene="tuf"
                     /locus_tag="ACHA6A_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8968512.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            59236..59592
                     /locus_tag="ACHA6A_000273"
     CDS             59236..59592
                     /locus_tag="ACHA6A_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8968513.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(59833..60837)
                     /locus_tag="ACHA6A_000274"
     CDS             complement(59833..60837)
                     /locus_tag="ACHA6A_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8968514.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            61003..61854
                     /locus_tag="ACHA6A_000275"
     CDS             61003..61854
                     /locus_tag="ACHA6A_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8968515.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            61871..62116
                     /locus_tag="ACHA6A_000276"
     CDS             61871..62116
                     /locus_tag="ACHA6A_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968516.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT        17242 a        13024 c        10691 g        21165 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
     1141 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
     1201 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
     1261 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
     1321 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
     1381 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
     1441 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
     1501 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
     1561 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
     1621 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
     1681 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
     1741 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
     1801 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
     1861 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
     1921 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
     1981 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
     2041 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
     2101 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
     2161 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
     2221 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
     2281 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
     2341 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
     2401 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
     2461 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
     2521 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
     2581 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
     2641 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
     2701 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
     2761 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
     2821 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
     2881 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
     2941 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
     3001 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
     3061 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
     3121 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
     3181 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
     3241 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
     3301 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
     3361 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
     3421 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
     3481 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
     3541 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
     3601 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
     3661 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
     3721 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
     3781 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
     3841 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
     3901 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
     3961 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
     4021 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
     4081 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
     4141 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
     4201 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
     4261 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
     4321 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
     4381 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
     4441 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
     4501 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
     4561 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
     4621 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
     4681 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
     4741 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
     4801 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
     4861 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
     4921 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
     4981 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
     5041 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
     5101 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
     5161 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
     5221 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
     5281 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
     5341 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
     5401 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
     5461 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
     5521 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
     5581 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
     5641 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
     5701 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
     5761 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
     5821 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
     5881 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
     5941 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
     6001 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
     6061 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
     6121 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
     6181 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
     6241 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
     6301 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
     6361 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
     6421 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
     6481 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
     6541 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
     6601 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
     6661 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
     6721 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
     6781 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
     6841 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
     6901 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
     6961 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
     7021 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
     7081 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
     7141 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
     7201 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
     7261 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
     7321 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct
     7381 atatatgaat ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac
     7441 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
     7501 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
     7561 gaagaagctg gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat
     7621 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
     7681 ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa aatttgctca aattgcaaaa
     7741 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
     7801 ttagaagtcc aagatctaga cggaaataca ttagaacccg tatggatgaa gatgtaagca
     7861 gataactaat ctgacgtttt ttataacaaa aaaacaaagg cggatgatac gataagtatt
     7921 ctccgcctct atttttttta ttcgtacaat ctctttttta cagtttataa aagcagtctt
     7981 ctcacccttt ttttaattgt tagatgccag tgtatgtaaa tcaccaatgt gtcatgacac
     8041 attggtgatt ttatggtatc cttttattat taactgacaa aggagagaat attttgaaca
     8101 ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa tgcaagattg ttgtcgacct
     8161 ctgctctttt attagccctt gatgtcatct tatacaagat agcgattggt cctgcttatt
     8221 ttcaaatcac attcggattt ttaagtatgg cgcttatggg ttatctatat ggaccgatct
     8281 gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt cacgatattt ggttcaggaa
     8341 cgtttcaagc tgcttttctt atcacggctt tccttggtgg atttattaat ggcttatttt
     8401 tatatcataa aaagataaac ctgccttccg tcatcgctgc ccaattattg attatgatcc
     8461 cagtcagcct ttttatcaat tcttggctga tgtctgtttt gtttggtgca gatttcaaag
     8521 taatttttct aggtagattc atcaggaata tgatccaaat ccctattcaa ataatggttg
     8581 tttattcatt tatcaaaact ttagaggcta gaaaagtgtt taaacgagct gtttgatttt
     8641 acgtactaag gtagtaaact gattttggat taaacaataa agcgtgcagt ctcaacagac
     8701 tgcacgcttt attgtttctc actggatcca ttttatttca tcaagtggat aatagacaaa
     8761 tcttgcttta cctagaatat cagttttatt gatcgtgcca aacgaacgac tatcttttga
     8821 aactcttcga ttatctccaa gtacaaaata attatcttcc ggaagcttgg attgtcccgt
     8881 tagctcttgt agtgtaaagt ccgtcgtata ggatgcattt gcatgatctg atttcaggtt
     8941 tttagttaga taaggttcct taactacttt cccattgatc ttcaattgat cattttggta
     9001 gctgacattt tctcctggaa gaccgattac tcgtttgata taaatcgtgc catctgctaa
     9061 ttgaaaaaca acaatatcaa atcgtctgat ctccgaaaat ttttccatca caaccatatc
     9121 accttgcttc aacacgtttt ccattgaatt gccttccact ggtaccggga tgaataaaaa
     9181 tccccgcaac atgaacgcaa ggaatacaga aacaagtaag tatttgaata caagccagaa
     9241 gcgattaata tagcgttgtt tttttgtcaa tctctatcaa tccatccctc tttttttcat
     9301 tctttcttta ttataagaaa aatatcaaaa aaatgcgact agatagctga aataaaaata
     9361 attctttcaa ttaggctatt ggacaaaaaa cttttttgat cataaagtac aaaactatct
     9421 cttatatgtc atgattattt tctaaatatt tttcccatgc ttcgtcaaag atatccatgg
     9481 acggcaaata gtcgacattc agttccagat aattagaaat ttcttcgtag ctagagctgt
     9541 gcttaggaaa ttggatgtct ttaccggctt ctgtagcgaa aacagataaa gcatcatgtg
     9601 tgccaccgcc tcgcagtgtc attaaataat gaaaaaagct tcgattcacc cttcgttcct
     9661 ccaattttct tcgataaagg cagcccggcg tgcatggttt cttttaaaat tttcaggatg
     9721 ctttttataa tactcttgat ggtcttcttc tgctagataa aatggttggg ctggttcgat
     9781 ttttgtaacg attggctgtt cgaaacgtcc actttcagct aactgtttct tacttttttc
     9841 agcaatgatc cgctgttctt ctgtcaaata aaagatcacc gggcgataat tgtcaccgcg
     9901 atcctcgaat tgtccgaaag cgtcagttgg atcagtttgc tgccagtaaa gttctactaa
     9961 ttgttcatat gaaatcaatg ttggatcata gataatctcc actgcttctg tatgtccagt
    10021 cgtttggctt tttacttgtt cataagtcgg atctggtaca tgccctccag tatatcctga
    10081 catcactgta tggataccag gaagcgtgtc gaaaggctgg atcatacacc agaagcagcc
    10141 tcctgcaaat attgctcgtt ccattatgat tccccctcta atactactca tccgatgatt
    10201 ttttattttc ttttgtcttg ctttcagtac tgctggtgct agttttgttg gtatttgagt
    10261 tgtttgaagc atcagaggta tcttttggca aatatagact aaaccgaatg tcatcattta
    10321 ctaagtcgat tttatctgct ttaatataca tcccgttttg cattcggaat tgatccaacc
    10381 taagcatgat agtcagatca tctgtattga cttcgatcca atctggtaat ttatagtttc
    10441 tcttgatcat attcatcaca tctttgattg gcaaactcaa tgttccgata gaaagacttc
    10501 tagcttttag ttggacattt ccgttttcca tcacatacgg atcaaaatat aaatagaaat
    10561 cgataggaaa acctagtact tcgaaagtac cattgagtag tgcttcattt tccaaataaa
    10621 atttatattc aatatcacta tctttttgat actcactaag gaaaaagctg atgatttgat
    10681 tgactttttc tttatttgaa ttgatcgtta agacaggatc tccttgtctt tccgtaagag
    10741 ctggtatttc tgggagttct ggttcccgat tagcaaaaat acggctgcct aaaaatacgg
    10801 tcgaaccaag tactacccca agaaggataa ggaaggcgaa tttccagtaa tttgttttct
    10861 ttgttttgtt tgaagatacg tcagatcttg ttttcatgtt tattgatcat cctccttctc
    10921 taaccattgg ttttgtgtct cgatcagctg atctctcact gcatttgcca tcaattgata
    10981 acctaaatta tttggatgga aatgatcttc ttcatagagc acattatttt tgatgccatt
    11041 tagattttct gtcgtgctag ttgtttgatc tgttccttct tctgtgacac ctacttcctg
    11101 attcaatcct tgataaagca aatcattgat tgggataaag tagcagttcg gcattttttt
    11161 tgtcaccgtt tctgttcctt tgttccaatt gttaacaatt gtctgcatgt cagtgatctc
    11221 aggaaagttc aaataaaatg gattgtagat gcctaaaatg taaataggtg cggttttatt
    11281 gagggatcga aattcttcca atagttctgt gacattttcc tgatagtttt tcagtggctt
    11341 tttaaaggaa ttgaccgtta acccgaataa attattttgg ataactttca tcaaatcatt
    11401 accgccaaca gtcagtgtga taaaatcagc tgttttgata ttgtttcgaa tttcttcttt
    11461 tgtttttact cgctttaaga tctggtcact tcgttcacca tttacaccat agttttctgt
    11521 ttcaaccgcg gtcaaattat aacggtcttt tagatcattt gcgacaaggg gaacgaatcc
    11581 tccttgcttg gtttgatctc ctactccttc agtcagcgaa tctccgatag ctatgtagtg
    11641 aagtttttct tttttatttg gatcttctct cacagtagtt actggtttca ttagtgggcc
    11701 agctttcggt atagaagcag ctaaacctgc atagatgatg atcgcaatta ataatgggaa
    11761 caaaacgagg atcaaatgtc gtttcgttaa tttcattttt tcacctcttg tattcgattg
    11821 gttctaatgt tatcaaaaat tttatcaaaa aggtagtctt tatgaaaaaa gaaaacaatt
    11881 attttcctaa ttttataaag aaaaagagaa taagctgcaa gcctttcagg ctgagggctt
    11941 atcctctttt cgactaatct acataataca tgattgcaaa cgcgtcttta ccagtatgcg
    12001 tagcgataac cggattcgta tgcagtacag ggatatgcat gtccgggaat aattcttgca
    12061 aaccctcttt gaattcttgc gccaattctt ttgcatccgc atgtgaaata ccgatttgtc
    12121 gcacatttgg caattctttc agttcatttt tcaaattatt aaaccattta tcgaacgttt
    12181 tctttcctcg ccccttagtt acagggatta attcagaatg ggcgaaattc atcaccacgc
    12241 gtatgttcaa aacactagac agcaaaccag ttgttcggct gattcttcca ccttttacca
    12301 agttatctag tgtggatatt ccgatgtata attttgtatt gtcttgtact tttttgactt
    12361 cttcaaggat tttctctact gttttacctt cttttgctaa ttcagctgct cgaattacct
    12421 gaaaagataa tccttggtca gtggtatcac tgtcgataac cgttactttc gtttttgtta
    12481 gattactggc ttgtcttgct gcttctactg ttccactcaa gccttttgtc atatggatcg
    12541 aaatcacttc actgccgtct tctcctagct catcatacat cgagatgaat tctccaattg
    12601 gtggttggct tgttttgggc agattttttg ctttcgccat catttccata aatctttcgc
    12661 cgtctagttg gtcatcatcg ggatatactg ttccgtcgat catgatggat agcggcatga
    12721 cacgaatgcc taaagcatcg cgtacatttt tttccatcgt acatgaagaa tctgttacga
    12781 tttttacatt tgtcattatt ttctctctct ctttctttaa caagatgcct tattcatgtt
    12841 aaactacatg taagacaatt tctattttta gtataacaga caaataatga tttttcatta
    12901 attacaccta gtaaaggagc cgttttcatg gaaaaaacga aatttaatcg aacgtatgaa
    12961 atattaaatg aagtgtttaa tgcagtcacc catggaattg ggactggatt gagtatcgcg
    13021 ggactagtgg ttcttttagt aaaaggtgcc cacttgcatt cctccctcca tattgtctct
    13081 tatgctatct atggatcgat gatgattctt ttatttttaa cgtctacttt gtttcattca
    13141 ttgattttta caaaagcaaa aaaggtgttc caagtattcg atcatagttc gatttttctt
    13201 ttgattgcag gaagttatac tcctttttgt ttgctgagta tccgaggatg gctaggttgg
    13261 actttatttg tattaatctg gttgtttgcg attcttggca ttatatataa agcgttgacg
    13321 ttgcataaac gggagagagt atcaaaagta tctacgatca tctatatttt tatggggtgg
    13381 ctctgtgtcg tggctgctgt tcctctttat cactctcttg gaatgactgg tgttattttg
    13441 atggtagcag gcgggctttc ttacacgata ggcgcattct tttatagctt gaattctatc
    13501 cgctatatgc atgttgtttg gcatttgttt gttatgttag gtgcaggttt tatgtacttc
    13561 tctgttttat tctttactta ataaaaaaaa taaagctatg gatgggacag ctctaaataa
    13621 cagctagtct atccatagct tttttatatc gtcaattcgc ctgtcccgct ttttacagga
    13681 ttctcattac gctgatacgt ttcaaattga tgcggataac gatttttttc atccatttct
    13741 ccttgactag tattgatcag tgaccaatca gaccagtcaa caggtggaaa ataagcatct
    13801 ccatcgaata catgatggat gactgtacgg tataatatcg tacaaaatgg cagaaattct
    13861 tgataaacag ctgaccctcc agcgatgaaa aagataccgt ctgtttcttt ttccagagca
    13921 agcacgtctt ccacactatg catgacatgc acgccttctg ctttatagga agaatctctc
    13981 gtcaaaacaa tcgtctgacg attcggcaac ggacggctac ccatcccttc gaatgttttt
    14041 cgtcccatca ctagtgtatt tgcttctgtc atttgtttga agaatttcag atcatttggc
    14101 aaacgccaag gaagacggtc ttcttttcca atcaatccgt tttcatcctg tgcccaaatt
    14161 gcagctaaca ttttctcatc ctttctgatt ctaaacagcg ataggagctt tgatcgccgg
    14221 atgtgggtca tatccttcta attggatatc ttccatctca aaatcaaata ctgactgctt
    14281 ttcctgattc aaaactaaag tcggaaatgc gcgtatttct cggctcaatt gttctttcat
    14341 ttgttcgata tgattgatat aaagatgagc atctcctaat gtgtgaacaa attcgccaac
    14401 cgctaaacct gtttcatgtg caattaggtg agtcagcaac gcataacttg caatgttgaa
    14461 cggaacgcct agaaacacat ccccactgcg ttgatacaat tggcagctta gccgtccctc
    14521 ttgaacataa aattgaaaca tcgtatgaca aggaggcaaa gccatgctag gaacatcttc
    14581 cggattccat gcagaaacga tcaatcttct agaatcagga gtctttttga ttgcttcgat
    14641 cacttgtttg atttgatcga taaagccacc gtcttttgtt tcccagtgac gccattgata
    14701 gccgtagatg tgtccaagat cgccgtatgt ttcagcaaag gaatcatcgg ttaggatctt
    14761 ctcgcaaaaa agcttgctct gctttttata ttcttcattg aattcaggat ctgtcaaaca
    14821 tctatggcca aaatcagtca tatctggtcc attatagtct ggacttttga tgtagcgttc
    14881 aaatgcccat tcatcccaaa tatggttttt attttgtaat agatagcgga tattcgtatc
    14941 tccttttaaa aaccaaagca attcgctttt gatcaatcca aaaggtactc gtttcgttgt
    15001 taacaaagga aacccttttt gcagatcaaa ccgcatttgg tatccgaata agcttaatgt
    15061 gcctgtgcct gttctgtctt ctttacgatg cccttcttcc aacaattttc ttcctaagtc
    15121 aagatacgct tgttccatcc tttttcctcc taatcttcta cccattcact caagtattcc
    15181 caccgtgcca ttttttcttc aagttgtgct tctgtttcag atacgtcgtt ttgcaattct
    15241 tgtaatcgag tgaaattatc tccttgatga ttcatctctt cttggagcaa tgagattttt
    15301 tcttcaagtt ctgcaatctc atcttcaatc gtttcccatt ctttttgttc catgtaagat
    15361 aatttcttct tttcagcagg ttccttgctc tgtatttttg gtttcgcttt ttcagcaggc
    15421 tgctgggtct ctttttgctt cagtaaataa ctggacatgt ttccgtaata aagttcttgt
    15481 tttccgtttc cttggaatac taataactta tctgctacct tgtccaagaa ataacggtca
    15541 tgggaaacag tgatcacagc acctggaaaa cttctaaaat aatcttccaa aatcgtcaat
    15601 gttgctatgt ccaaatcatt ggtcggttca tccaataata aaacattagg ttgctgaatc
    15661 agcagtttca acaaaaataa ccggcgtttt tctccgcctg aaagtttgcc tatcagtgtc
    15721 ccatgcataa atcttggaaa taaaaatcgt tcaagtaatt ctgctactcc gatttgactg
    15781 ccatctgctg tttttgcttc ttcagcagct tcttgtaagt aagcaatcat ccgtttatct
    15841 ggtgccattt cttcattttc ttgtgtataa tacgcaagat gaaccgtttc accgatagac
    15901 attgtaccgc tatccagggg aattctgcca gctagaatat taagcaatgt cgattttccc
    15961 gcaccatttt taccagtaat acctagtctt tcccgagatt gaatcaataa atctaaatgt
    16021 tctagcagtg tttggtcatt tatacggtaa ctgccatctt tgatttccag tacttttttc
    16081 cctaaacgtt gtgtcgctag gttcagctct aaatcatctt cttggtttac ttggtacaag
    16141 ttctccttta gatcttcgaa acggttgatc cgtgcttgct gcttggtgcc acgtgcctgt
    16201 acgccagccc tcatccaagc taattcctgt ttataaagcc gcttgcgctt ttcttcttgt
    16261 tctacttcta cacgatcacg ttctgctttt tctaagacgt aagcttcata atttccttta
    16321 tactcgtata attttccaaa tgaaagttcg aagattcggt ttgctactct atctagaaaa
    16381 taacgatcgt gggtaaccat caatagtgcg ccacggtatt gtttcaaata gttttccaac
    16441 cactcgatcg cttcataatc taaatggttt gttggttcgt ctagtaatag taaatcgggt
    16501 tcatcaatca acacttgcgc cagactaact cgttttttct gaccacctga taaagtactg
    16561 atttttttat ctaattcact gattcctaat ttttgcaaaa taatctttgc gttggtatct
    16621 gtagtccaag catcttcttt attcatttgt tcttctgctt tagcataacg tttttgcaca
    16681 tcagggtctt ctccgttttc ccctagttcg atcaatgctt cctcatatgc tcgaacagtt
    16741 tggatcaagg ggctgtttcc ttgaaagact gcttggagaa cagttaattc atcggaaaat
    16801 tcagctgctt gagaaagata accaatacgg taatcagttg gataaaacac tgtttgccga
    16861 tcaccgtcgc tgctatcgat accggcaata atatttaaga gacttgtttt acccgtcccg
    16921 ttcgtcccaa tcaacccgat acggtctttt tcgtggacca aaaaagaaat ctggtcaaac
    16981 aaatcttttt caccataagt ttttttgagg tcagttactt ttaattcttt caacatgttc
    17041 cctccttatc ttggatattg tatcataaaa atagtgaact tcaggaaaat ttttttggta
    17101 tttgttcttt ttgtgttctc aaaaaaagaa caggaccgat tcgatcctgt tctgatatgt
    17161 tttgtttttt ataaaacttg aaaattttta cctttttatt ttattggact cgtttttttt
    17221 gactttgctc gaaacgcaaa tcggaaagaa aagttatcag atggtttgtt catcgtataa
    17281 gattctaaca aattgatatt cctgcgatag ctgttcgcag aaaacgtcgt gattttttca
    17341 gcaacttcgt attcatcaat gatttttctt tcaagataat aacctcttag catttttttc
    17401 gtaaacagac tatcttgctg gatcatccac gaagaaatca aattccccga gttcattttt
    17461 attccttcaa gtttgcgttg ctggataaaa aattgaacca tcgtttcatc ataaatatca
    17521 gtcaagttat ctaaactttc aataatcaac tgagtatttt tttgatatgt ctctctgatt
    17581 tcttggatat cttcatcact aataaatgtc tgacgatcag catatcttct ttgccaaaac
    17641 attttcggat ggagaacgat tcgcattaac ctacggacaa caatgaacca aaagccgata
    17701 aaagacaaaa tctggcttgt gatagaatgt tcgaatcgag aaagaaaacg ggagtaaaaa
    17761 cgataaccat tagaagaaag cttgcccgaa cgataacttt catccaatcc atcccgttcg
    17821 atcgacaaaa tcagtgcttg tatttcttgt acttcttgct tttcatcatc ggttaaatct
    17881 tccaagtaca gatcacgtat ccgttcttga taattttcta tcactgcttc agtggctaaa
    17941 aattcttttg tattacgatc aatttccttt atttcttttc gcaaaacttc aacgacctct
    18001 tctaaaatca tcaaagcatt caaatccgtg cttttcgctt cctctgtttc cgtcagcata
    18061 ggcaagacaa tgatccctat gaccaaagtc actaagatga cacccgccgt cagaaacaaa
    18121 agcaaagacc gctggtaaaa catcatatta ttgattgaaa atggcaaaat aaaaatcgta
    18181 gccaaactga ctgttccctt tacaccgcca aatgttaaga tcaagatctc gttcaactgt
    18241 tttttgaact ttttactgcc atctttgaat acataaaaaa gactgataga aatgaatctg
    18301 atcacaaaaa gcatgacaga gattaatacg ataatcgcta ataaaagacc gtttggatat
    18361 aaaccgtctc cccatactgg cgagaacact tgcgtcaatt cgattcctaa aaatataaat
    18421 actaatgcat tcagtgtaaa agcaatagtt gtccatgtac tgtgggacaa gctcgacaat
    18481 tctgcatcaa acacagagat tttcctaaaa ccagaagctt ggagaatacc tgctgccact
    18541 gcagcaatga ttccagatac atcaaatgcc tctgccaata cataggctag aaaaggaagc
    18601 aaaagttcga tcagcagata ccctgtgaca tcttgtgctg atgctttttc gatcaaatga
    18661 attatttttt gtttgaacca gacaagcaaa aagccgatca aagctccccc aatactagaa
    18721 actactagac tcatccccgc gtttactgct gaaaaagatc cggtgatcag cgcaccaaga
    18781 gcaaactgga aggcagtaac accagatgca tcattcaaaa gaccttctcc ttccaaaata
    18841 tgcatcgcct ttttagggat attcactcgt ccagaaagag aactgactgc taccgcatct
    18901 gtcggaccta acgctgctcc aaaagcaaag caggcagcaa gtgggacact tggcaacagg
    18961 aatgataacg tcgcacctac cccgacaagg gtcaaaatga ctcctccaaa tgccaagaac
    19021 aaaattgtcc cgaaattttt gagaatcgaa gaaatatctg ctttttctcc ttctctaaat
    19081 aacaatggtg caataatcat aactaagaac aattcaggtt caaaatcgat tgattgtccc
    19141 cactctgtca gacctaatac cactccaagg aaaatttgga tcagtggagc tggaatcatt
    19201 ggcaaaatgc ggctagctat gttcgataag gtgatcgtta ctgcaaatac aataactaac
    19261 tcagcaaatt ccatcgtcca tcccctttat tttttatttt gtgtaagcat ttttaaaaat
    19321 tgttcttttt tctttaaaat tttggtttcg actacttcaa cctgtgtttg gttcccctct
    19381 aattcacact gtacacgttt tgactccaaa cgaagcagat catgtttcag acgttccaac
    19441 tgacgataca tcaatatatt tcttttacgc aacttacaaa gtatttgacg gcggtcatcc
    19501 atcgaaaggg cgccatagcg atcatgcaac agtttttttt gttgttcaaa actctctctc
    19561 attttccaga tctcctatac acaaacttcc atcattatag caaagttctt tttggatgaa
    19621 aaatatttca ttgtttcatc gtttattgct agttagaaat ttccataaat ttttcaacgc
    19681 ctcattctat tccaaatagt atcagatatc aagtaaaact taagaaagat aaaagtttct
    19741 gacaaaagga tgtttacttt cccattcctg cctttcttgt tcttttatca caacaaaaaa
    19801 atggggctgc gacaaaaatc ttgtcacagt cccttattct gaataaacgg tgtggaagag
    19861 acagcctatc agtagtaata agacacatct ctttcacagc ctcatttttg cgtaaaaatc
    19921 gatttattcg aaaaattccc cgtatacatc ttctacacgt tgatctggat tgatcacgat
    19981 atacaagtta cctgtttttt cgctgatttt tgttgattga tattgattgt ctaatccttc
    20041 tgttccttta tctagatatg ggaagtacac aagatcaggt gtgccatttc ggtaaaggat
    20101 ttccattcgc tcgatatctt cgtatttcaa tgcgcgatta aacatgccca tttccagtcc
    20161 gcctaggttg atatcatgtg tcgcaactgc gtccccttca tggaagattt cgattttaaa
    20221 tccagcacac ggatggattt caacaaattc actgccattg attcgtccaa aacttgttgt
    20281 gatttgtttg atccaaagat cgccgatatg acggcggttg atcgtccagg tttctccatt
    20341 tctaaaataa aattttagcg ctgtcatttc tttactcatt aaaaagactc ctttatgatg
    20401 taatttattc ttgtactacc tcagtttagc acaaatttat gtaagtggtc acatcttatg
    20461 ttttttcttc taatgacgta tctgcccact ttaagtagcc aacaaagctg agcagataca
    20521 gtctagtgtt ttttaaatca atttttcttt cgataaaaaa tccaaaacct gttttttatc
    20581 attttttatt ctttgttcta cgacggcttt ttcagctaat tgcaaagctt cactaaccca
    20641 cttaccaggt ttttttccag tttcttctag caagatttta ccatctactg ctaattcgct
    20701 taatgacttg ataggtaaac gatcatagga ttctcctacg gcatccatct gaggtgtttc
    20761 gtcaaaatag atcatcaatt cttctacttg cagtgcatgt tttttgccta agaagtacaa
    20821 ttcgaatggt tccaaagctc cagtttctcg ttgtcgtagt ccttggacaa gtgcttgaac
    20881 gtctttaatc atttggttag aacacttcca cgatttcaaa aaggaacgaa tctcattttc
    20941 ttttagatcc agttggctga tcaataatgc ccatgcttgg ctctcttctt tcaatgggac
    21001 ggatggtaat tcagcaaaac gtaaaagtgc ttcgccttct tctttcaaac cgggacagca
    21061 aatgtagcac tctgtttcaa taaatgcatg gattcccgcg gcccgttcat ttcccaagag
    21121 catcttgatg aattccacat ttatacgttc tacagatatt ttacctaata gctcgtggtt
    21181 ttctttgatg gcgtagaagg tttcttcctc taatttgaac cctagttgac tgacaaaacg
    21241 caacccgcgc atcattcgta aggcatcttc atggaaccgc tcatgaggat tgcctacagc
    21301 tcgcaagatc tgttcttcta gatcttgcag accatcgaac aaatcgatga tttctccatc
    21361 ttctttcaaa gcaaacgcat tgatcgtaaa atctcttctt ttcaaatctt ctgccaacga
    21421 acgaacgaat tcgacatgat ctggtctacg gtagtcctga taagttgatt ccgtccgaaa
    21481 tgtggtgatc tcatattgtt catcattttc taataccaac acagttccat gttcgatccc
    21541 aatatctatc gtttttggaa aaagctgttt gatttcttct ggaaatgcgc ttgttgcgat
    21601 atccacatca tggattggct ggttcagcaa gatatcacga acacttccac ctacaaaata
    21661 agcttcatat cctgcattct ctaatttttt cagaactgga attgcctttt ggtattccag
    21721 aggtaattgt tttaatttca tttttccaac ctttcctgcg tgtttcttgt cctgagataa
    21781 agactcagtt gcatggtgac tgtttgacaa gtccgtttca gagtcttcaa ggccaggacc
    21841 acttcatctt gtttactcgt ccttacgctc aaagcgatta tggtctcttt gattgaattt
    21901 ttccatgttt ttttcaaaaa cttcagttaa gtcgatattc aatgaattag ccatgatcat
    21961 cgtaacgaat aaaacatcac caagttcttc tgctaccgag ttaactggtt cagaagcctt
    22021 tttgcttttt tctccataag tgtggttgac ttctcttgcc aattcaccta cttcttcagt
    22081 taatcgagcc atctgagcta atggagagaa ataccccacc ttgaattgct ggatatattc
    22141 atccacttcc tgctgcatac tgtataaaga acgcttttta tccatctttt tccctccaaa
    22201 actatcttac caaaacttta acgcaaatcc tagataagag caattgagaa aatcgaattt
    22261 ccatgctaca tttaggaagg aatttctaaa tggaaattct gtgaaaatac ataattgaaa
    22321 gctggaattc atatggtcaa acgaaaaaca gtaaaagaag ttctttttat cattatagga
    22381 acaagtattt atgcatttgg tcttgtttac ttgaacatcg ctaatcattt agcagaaggg
    22441 ggagtctcag ggatcacctt gatcttaaga gctttatttg gtattgatcc agcttatacg
    22501 actttattaa tcaacatccc attgatttta ctaggaggaa agatcctcgg taaacgttcc
    22561 ttggcctata ctgttctagg tacactttct ttgtcattct ttttatggat ctggcaaagg
    22621 gttccactag aaatcaattt acagcatgat ctactgatcg tttctctact tgcgggttta
    22681 gttgcaggtg taggaagcgg gatcgtctac cgcatgggtg gaacaacagg cggcagtgat
    22741 attatcgctc ggattttaga acaaaattac ggcatcacta tggggcgttc cctattggca
    22801 tttgatatca tcgtcttact tgcttcatta acctatatcg acttgaaacg tatgatgtat
    22861 acgttgattg tttcgtatgt tttcagtcgt gtgattgatt cgatcttaga tggcggatat
    22921 tctgcaaaag gtctacttgt cgtctccaac aaaagcgaag agattgcacc actactgatg
    22981 accggtctgc aaagaggtgt gactttcctt tctggagaag gcgcattctc tggtaattca
    23041 aaaaaaatca tctatatggt cgttagctct agtgaattaa ctgaagctaa gaggatcgtc
    23101 cacgagattg acgaaaaagc tttcctttca atcatcaatg tgcatgaagt ggaagggcaa
    23161 ggctttactt atttgaagcc acagacaaga ttactgaaaa aactataaga aaaaagacaa
    23221 agataggcta tgaggaaaga ttccacatcc agctgacctt tgtctttttt agtactatac
    23281 tatttttaac taattgttat gttcacttac ccggttactt ttcttttgtg tctgcaaaag
    23341 gatctttcgg ttaacttcat cagataattt caaaaaagca tccctatctc catcttctag
    23401 tgcttgatcg atttgagcat aaagcatttt tagttttgcc tcttcttctt tttcttgaaa
    23461 atatcgttca attaattcag tatcttcttc actgactgaa gaatcccaag aagaaaatgg
    23521 attgtcttct aagatagata gatattctct tgtctgccaa gcattttcga atatacattc
    23581 cacatataaa ggctttttcc aattcaaacg gatttcatga aaaatctggt ctgtgtccgt
    23641 aaattctttt ccctgcaaaa acaatttcat gggttcccct tcaacagata gatcactgat
    23701 ctgcagtcct cgatccgcaa aattcgcccc ttcaacaaag tggacattgc ttaaaatcgc
    23761 ttcatgattg gcaagataat tcaagatcca aaaaacttct cttcggctaa aagatacatg
    23821 attgaccatc cacgttaaaa aattcttctt ttcccgtaca tcaattagca tttttctcac
    23881 ctctctgtta ggtcatctaa taatgacagt gcctccatat ctcctggttc aagtgttaaa
    23941 taataagtca ataattcttg cgctcttttc agctgccctt cttctcttaa gaaaaaggca
    24001 tactctttca agaaatctgg ttcatgtgat agttcatgat aagcctgttc atagtgtaca
    24061 gctgcaacgg taaagtcttc taattcatta taagcatggg ctaggttcca ttctgcataa
    24121 ggattagcag tttcttccat ctgattgatc acttgtatca catcttcata tctttcatca
    24181 tccaagtaaa gattacttaa agtaagtaat gtttcatctt gtttgtctcc tacttctaaa
    24241 gctttcagta aaagttcttc tgctcgctgt ctatcatgca gacggaaagc attttccgag
    24301 gccaggtgat aaagttcgac ttggtaagga ttttctttga ttccttcttc taacactgtt
    24361 tgtgcttctt caatcatttc ttcttcttga agagcttgtc ctagatatag atataacgat
    24421 tgataatgcg gattaacttc tcgaagttct tccagatagt tgattgcctg ctgattttct
    24481 ttcaattgaa gattcgtaaa agctagctgg aacagtcgat catctgtcct ttcttctttc
    24541 agtgcttctt ctaaaggtac gatagctgct tcgaattctc cttgcatact taaggattgt
    24601 cctaaacgtt cctgcatcga gattcctgaa atttcattta tcccgcttgc caacaatgtt
    24661 tcataaacca ctgctgcctc tttgaaacga tcaactgaaa aatacaattc agctagcgca
    24721 aactggatca atggttcatc aggaagcaac ccagctgctt ctttcaattt tgcttcactc
    24781 acttcaggga ttccgataac ttggtacaaa tcagctatcg ccaataagct ttgcggatag
    24841 taatcacttt gcttatccac tttttccagg tattcaaaag ctaagtcgat ctcattatct
    24901 tcgattgcaa tttctgctaa cgggatgttc agtccgtcat tttctgggta ttggttgatc
    24961 agttgttcaa aaatctgttt tgcttcgatc aaaaagccaa tagccagcaa ttcttcgcct
    25021 agttcagcta atatatctgc tgggtcttct tttaatgctt tttctaaaaa gatttgtgct
    25081 tgtgacaagt cttcatctga taaagcttgc aacatttttt cgctatttgt attcatttca
    25141 attccctctt atctcactga cgatttgttg aaataccgtc attgttctca ataattcttt
    25201 ttcgtttgga tgttggtatc cgtctattat acctattttt tctaaaacaa gtattttttt
    25261 gcttttttct gccaaaaaaa gaactttttc caaatactcc atctttgaaa tctgttccag
    25321 ccagtggatt ggatagccaa gcaagtccag ccacacaaaa aagtttttta gttggaaatc
    25381 aaaaggatat gcttggagat tccacacgaa atgaaacatc attcctaaaa attttttcat
    25441 actagcagaa agtagatggc cattttcggt cagataaaaa gcttgttcaa acagtttacc
    25501 ataatttgac aggttttctg cctctaattg ataaaaatag atcatttctt cgatcatccc
    25561 ggcaaacggc cgagtcgtta attgcttttt atttggatag tttttataca gattttgcaa
    25621 aaaacgatga tcacaaatca ttccgcaaca gataaaagtc aaaaagtcaa tctgctttcc
    25681 gtccacctgc cgctcgctga ttgtttgatc ataaacgatg cgttctggca gattgatggt
    25741 ttgcagcact acacgatttg gtagctgcaa aatcgtccgc tgcggtgtta atgctcggct
    25801 tagagaacgg atcgatacag ggatcatcca tagtgatgaa tcaagggttg tatgtttttg
    25861 aaaaaaacct gccagatcca tgatcccttc atttccaaat ccgatgataa acatggattg
    25921 attttcggga tagcgtttga cgaaatcaag caggttcttt aattctgtta aatgattgca
    25981 ttgagtgttg gcacaaatat accagtcatt gtttgcttga tttgcaaata acgggttgat
    26041 cttatctgca aacaaatcat aatagcgctg attcgttaaa aataagattg gctgatcaat
    26101 ggaaagttct cttagctgac tgactagcgt ttctccgtaa aacactttcg tcaacgcatc
    26161 ttctacttga taatcaagtt ccatacgcat ccctcctctt cttattttta ttttaccatt
    26221 agtaagcaaa ttctagaaaa gataagagca aagctcacct cataacttgc aaataaataa
    26281 aaattaaata aaatacatgt gttttctatg tatgacatac ggattcggaa tctctgtctc
    26341 ttatttattc cttttctgct aggaaataaa aagaaaagca cttgtagccc tagagccaca
    26401 agcgcttttt caactgtctt atttaacagc gtcttttaat gctttacctg gtttgaatgc
    26461 aggtactttg ctagctggaa tttcgatttc ttcaccagtt tgtgggttac gacctttgcg
    26521 agctgcgcgt tcgcgaactt caaagttacc aaaaccgatt aattgaactt tttcgccgtt
    26581 agctaaagca tcttgaatag ttgaaaatac agcatctact gctgcagttg catctttttt
    26641 agttaaatca gtagctgaag caactttttc gattaattct gctttgtttg ccatggatag
    26701 attcacctcc tccacaagag atatgataga aatatcattt agttaaaact ttctgagtgg
    26761 tccagaagtt taaaaagaaa tattgattaa cttcaatatt ctgttatcac gatatcatat
    26821 gaaccgctta atagcaaggt ttttaccaat tttcacatgc tttttttagt catagaaacg
    26881 taacaaacga aactgtcttt ttcttttgaa aaaaaaacgt cgatatggta aaatgcctat
    26941 tttctcctac gagggataat tcggatggac gtcccttcaa aagtaaatgc ttttctgatt
    27001 tgattttcta agaaacgttc ataagaaaaa tgcatcaatt cctcttcatt gacaaaaacg
    27061 acaaatgtag gtggtttgac agcgacttgt gtagcataaa aaatcttcag gcgttttcct
    27121 ttatctgttg gtgttggatt gattgctaca gcatccataa tgatatcatt caacacagcc
    27181 gaagagatac gaagattctg gttcatactt acttgttcga tcatttcagg cagacgttcc
    27241 aaccgttgtt tagtcactgc agaaacaaaa acgattggag cgtaatcaag ataccggaat
    27301 tcatcacgaa tctcttgttc gaaatcacgc attgtattgg tttctttctt tacaagatcc
    27361 catttattca cgacgataat aattccacga ccagcttcat gggcgtaacc cgctactcgc
    27421 ttgtcttgtt cccgaatccc ttcttcagca ttcagtacca tcaaaacgat atcagaacgg
    27481 tcaatggcac gcattgcacg catcacgctg tatttttcag tattttcgta tacttttccg
    27541 cgtttacgca taccagctgt atctatcatt aagaatttct gaccactttc agaaacgaag
    27601 tgggtgtcga ttgcgtctcg ggtcgttcct tcaatatccg aaacaattac acgttcttct
    27661 cctaaaatcg cattgatcaa agaggattta ccaacatttg ggcgaccaat caaactaaac
    27721 ttaatcgtgt cgtcgtcctc ttcttcagtc tcttcagaga aatacttcac tgcttcatct
    27781 aaaatatcac caatccctag tccatggctt cctgaaacag gataaggatc gcctagccct
    27841 aaggaataaa attcatagat atcgtttctc atttctggat tatctacttt attgactgct
    27901 aaaatcactg gtttattact tctgtataaa attttcgcaa ccatctcatc tgcgtctgtc
    27961 acgccttcgc gtccgctgac tacacaaatg attacatctg cttcttcaat cgcgatttct
    28021 gcttgatgct tgatctgatc cataaaaggt tcatcgccaa gatcaattcc gcctgtgtcg
    28081 atcacgctga attctcgacc taaccattca ccttttgcat aaatacgatc acgagttact
    28141 ccaggagtat cttcgacaat cgagatacgc tcaccagcga tgcggttaaa aatcgtagac
    28201 ttaccgacgt tcgggcggcc aacgattgca attgttggat ttgccatatt atttcctcct
    28261 cacaagtttc caatctcact tagtttaacg aatgcattgc caactttcaa gcatagattg
    28321 tcaattaaac gaagtatttt tattccttat tgaaatacta atcgcttttc aaaagaaaaa
    28381 ctgagacaaa agcagctgct ttcatctcag tcacagatac aaaatcaatt atttttcaga
    28441 atcatcggtt gctttttctt ctacttcttc tttcaacgca tcacctaaaa tgtctcccat
    28501 agtaaatcca gtattttctt ctggtaattc ataagactca acattttttg gttcttcttc
    28561 tgaagctggt ttttcttcta aagctttgat actcaatgca atacgatgtt cttctggatg
    28621 gatctctaat actttcactt tcacttcgtc accttcatga agcacttcat gtggtgtcgc
    28681 aatatgttta tgtgaaattt gagagatatg gactagccct tctactccag ggaacacttc
    28741 aacaaaggca ccgaagcttg tcaaacgttt aactgttccg tctaatactg tgccaactgg
    28801 agcttttgta tcgatttctg accaaggtcc aggctgtgtg tctttgattg acaaggaaac
    28861 acgttcttgt tcaggattga tagacaacac tttgacattt acatcatcat tgacagccaa
    28921 tacatcactt ggttttgcta catgactatg tgaaatctct gagacatgga ctagaccgtc
    28981 aattccgcct aggtcgacaa atgcgccaaa atcagtaaga cgtgcaacac gtccactgag
    29041 aacatctccc tcatggatag ttgaaagaat ttcttttttc ttttcttctc tttcgttttc
    29101 cactaccgct ttatgcgata agatcaaacg attttctgat ggttcgattt cgatgatttt
    29161 aaagctcaat gttttgcctt tatagtctga aaagtctgca acaaaatgat cttccaccat
    29221 tgatgcaggg acaaaaccac gtacaccgac gtcaacgact aaaccgcctt tgacaacatt
    29281 tgtaacaggt gcttcgatga ccttgccctc ttggaaatct ctttcgatat cttcccaaac
    29341 ttttttagca tctaaacggc gttttgaaag tagatagctg ccgttttctt tatctttgcc
    29401 gatcgtactg ataacgacta aatctaatac atcaccgact tttactactt cgctgatatc
    29461 ttctaccggc aaagttgata attcttttgc cggaacgact ccttcaactc cagccccttc
    29521 gattccgaca atcacttgtt tgtcttcaat gactaaaact tcgcccttaa caacatcgcc
    29581 aacttttact tcttgtacgc ttttcattgc gtcttccatt gactggttgt tctcttgatt
    29641 ttgatcaaat tctgtcataa cacttgtcct cctaaccaac attctgccgt aagtacggta
    29701 ctgtttttag tttaattgat tattcaaaaa aaataaagcg atttcgttcg ttttccttga
    29761 aaaaacaatt gtttttcatt ttttttctta atttaaaaaa gaagataacc ttttttcaaa
    29821 acaacagctt ttattgctgc aacgacttct ggaatcgatt tgccagtcgt atcgatacga
    29881 acagcatcct ctgcttgctt cagcggagag acgtcccttg ttgaatcata atggtctctt
    29941 tcctgtatct ctttcgtcag tgtttcaaga tcagtcggaa ttcctttttc ttggttctct
    30001 ttgaaacgtc tttctgcacg ctcacttacg cttgctacta aaaagatttt cacttctgca
    30061 ttcggtaata cagctgtccc gatgtctctt ccgtccatga caacgccacc gcgttcccct
    30121 atttttcgtt ggacagcaac taattcttct ctgacttttc catgtgcaga gacttcagaa
    30181 acagcttttg tgacatctgg ttgacggatt tctaaagtca catcttttga tgctatgaat
    30241 acatgctgtc catcttcttg ttgcgaaaaa gagataggat actttttgat cagcgaaact
    30301 agcccttctt catctgaaaa agatacattg ttcgtaatag ctaaataagt tactgcgcga
    30361 tacatggcac cggtatctgt ataaatataa ttcaattctt ttgctaatat cttggcaacg
    30421 gtacttttcc cagatgaagc aggcccgtcg attgcaatac tgatttgtgt catgatataa
    30481 ctcctttttc tcagaaaaaa agccgcaaag cgcggctatt attatttaat tcttaattct
    30541 tgaccagggt aaagcataaa gttgtttgga tctaagccat tcaattcaaa caattgatca
    30601 gtggtgatcc ctgcacgctc agccacttgt tttggacctt cgcctgcttg aacagtcgtt
    30661 gttgaaccgg ctgcagctga tgaagaaggt gttgtttctg ctgcattgct gcttgaatcc
    30721 cctgcttgat aagttgcctc acttgaggaa gtcggtgttg tctctgctgc aatactgctt
    30781 tctgacgtta cacctgtatc ttgagacaca gtagattctt ccgcgattgt actagattct
    30841 actgttgaag tctcagttga ctcctctttt gttgtacttg tagaactttc tactactgat
    30901 gtcgaagttt cagaagaagt tgctgctgtt gcattgttgc ttgatccatt tctgatccag
    30961 aaataagcac ctgctggaat agcgatacat aagaccagta aaatcaaaaa tagcgaaaga
    31021 aataatgtat ttcctttctt ttgttgtctc tgagaacttc ttgatgaaac ttcatcatct
    31081 gtatcgtaga ttggttgttc ccatggttct tgtgtttcgt tattttctgt agattgatgt
    31141 ctatcttttc tgctcactgt tttacctcct aaactctatc gtgtgtatta taccatagga
    31201 aaaagtgtct tgtcctaaaa aatcaatgtt ccatgttttt tttaaataaa tttagtaaaa
    31261 tcgttcccca atgaagttgt tcagtcttag aaaaaagctt cctctttggc agaagttctg
    31321 cttttgctcc atcaatatca caacaattct gcggcttttc ggagagtttt tcgccaaaat
    31381 aagctaagat gaattttctg cggcagtttt cttcattaat atagcgaagc atctgctgca
    31441 ggcggaattc tttttctttt tcctgacgtt tgagccgttc cacccaattt tctggttttt
    31501 cagactgctg gatcagctcc atccattttt tttgcagctc atcaaaggga gcttgctctg
    31561 tggcatattc caaataaagc tcaaaacttt gtcgctgttc tctagaaagt tgattaaaga
    31621 aataatggat acgttcatcc ccactttgat aaaaaagaat tgctgcactt tcttcctgat
    31681 ctctgcctgc tcgaccgatt tcttgtacat agttttctag actgtctggc aaatcataat
    31741 ggaccacata acggatatca gatttatcga tccccatacc aaatgcattc gtagcaatga
    31801 gaaactgcag ctgatttttt acaaactgct gttgaagttg tcttctttga gctgcatcaa
    31861 gaccaccgtg atagtatcca actgtaaaac gttccctgaa taaatgatac aatcgttcga
    31921 cttctttttt tgttgcacaa taaatgattg ccgctccttg tgctctttcc atgaagtgtt
    31981 caagttcttg ttctttttgc tgcgttttcc gaacaaaaag cgagatattt tgccgattga
    32041 ctgattgtct gatctctttt ggtggtcgat caaatagtac atgacgtata tctcttgcta
    32101 ctaaatctgt tgctgtcgcc gttagtgcca gtgtgaccgg gtttcctaaa agctttcgga
    32161 tttttcctaa ctgttgatat tctggacgga aatcaactcc ccattgagac acgcaatgag
    32221 cctcatctac tacatataac gcaatatttt gttttttcag atgctctaaa agagaaggat
    32281 tagtcaacat ttcgggactc aaaaacaaaa atttatattg cgatagatga cggaggacgt
    32341 atcttctttc agattgcgaa agtaaactat tgaaagcagc tactcgtttc tcccctcgtt
    32401 tttgtaattg cgtcacttga tcttccatta gagaaagcag cggtgacacg atgatgacca
    32461 gaccttccat taaatacccc gtcaattgat agcaaaggct ttttcccgtt cctgtaggca
    32521 aaatagcgag agtatcttct ccatcaagca acgcctgaat aatttcttct tggccgggac
    32581 gaaatgaatc aaatccaaaa tattttttta attcatttat taacattctg tatcgcttcc
    32641 tttctcaata gatagatttg atacaaacga aattctccat aatctaattg tccatctaca
    32701 ttccattcgc taaagcgcca ctctcgcacg gaatccttat ttggttccaa acgttcgatt
    32761 gtttctgcag agagaatcca ctcgtatgga aaatcatcaa aaaacaaggc ccactcaata
    32821 agatgatctg ttactgttcc tctttttaaa tgacggattg ccatgatctg gtcaattgtc
    32881 tgaccattca taaacatctg cttggtgatc atcatgcttt gattgaaatt ttgctgtaat
    32941 aatgaatcga tcaaagcgtg ccaaagcgaa tctggccgtt tttcgatttg tgctaacaaa
    33001 aggtggatac atctgctttg ttgcagataa accgctgact catcattgtt ttttgccagc
    33061 tgataaggaa gcagccctgt tcgctcattt ccagataatt ggttagctaa aaaatctgct
    33121 gcttcttctg gtatctccga aaacagctgc accaaactct catacgcagt atcaatcaaa
    33181 atttcacgtg gcaattggct ttcactaagc cattttttta gttggaaagt ataaaaagga
    33241 cttgtctctg ctggaagata gtcttgaata cccgttgcca gattagaaat tacttgaaca
    33301 gcaaatttaa tgagccgcca actagtttcc cctgttctgc catagcggtc aaaacgcaaa
    33361 ccagtatgtt ctatttgatg ttcggacaac atatcagccc ctgcaggcgt tagctttgct
    33421 tcattttcat ttatttcgat ccaaccttgc tgcgcaattt tttgcaaaat ttgataaaac
    33481 ttatcctgct tcaattctgg aaaactgtta tggataaaca aaagttcgtg ggtaaatcca
    33541 taaataagta ctgaagtcgt tcgttttccc acaaggagtt ggtaaagaga actaatccgc
    33601 agcttgtgtc ctgatgaaaa tagcgataaa ataaaaacaa tcatattatt ctccttaagt
    33661 taggatggtt agaaatgtca ctactatgta aacttgtacc tgaacgctgt attgcatgcg
    33721 gtctttgtca aatcgaagcg ccagatattt tcgattatga tgaagaagga atcgttttat
    33781 ttgcccaaga accagaggct catcaacagt tcgtgatgcc ggaagaagaa acatatgtaa
    33841 aaaaagcgta tcaaaaatgt ccagttcgag cgattttatt agaaaataaa atataaatat
    33901 aaacgaaact ttaaaaacgt acactaagtt ctctaaactt gaaaataagc cgggatctgt
    33961 ttctcttgtt ccgtgaaatc ccgacttatt ttgaaagatt catttgctat ctatagttaa
    34021 ttgactagtt ttttcacgga ccctatagga cccatttata ttatttcaac tttttcagcc
    34081 agttttccaa tgtattgacg atcacagttt gctggtctgc gttagaaatt tctgcctctc
    34141 catctccttt ttgctcacca tagctgccaa aacctccatg atttccgcca ttgattgata
    34201 catactctgt tctttccggt aaatactgct tagcttcttg ataagcttcc tgattcaata
    34261 ctttatcttt tgtcgccgtg atcgataaaa caggaacagc tgattctgtc aatgttcctt
    34321 ttttgtcggg ataacttgcc atgaagaata ccccttttaa atgtttgtct gaatgattct
    34381 tattgacata acgacttgcc attacgccac ctaatgaatg gccaccgatc acataatcgg
    34441 aatttggaaa atcttctttg accttatccg ctttgtttcc gcccatcact gctagatcaa
    34501 gtggcatttt gacaatagcg actgaaaagc cgttttttgc taaatcttgc gcccaaatac
    34561 tataactttc tggctctacg agagcaccag gataaaaaat aagtaatgga gactttccat
    34621 gattcttttt ctctccttca aaaaataagt aatcttcttt gttttttcct tcttttacca
    34681 acacttgcgc ttctgacgat ggttcgtacg tattttgctg tatatagtaa taaccggctc
    34741 caccgagtaa tcctaaaatg atgactaagc caattagtat tcgtctaaat attttcactt
    34801 tctcccacct tttctatctg agtgacccct ttttcttaat caagtatgat tataccttaa
    34861 aaaaataaga gagaaatgca aactttccag catcaaataa aaaaagacgg aatatccatt
    34921 tgaatatccg ccctttttgt atgtttattt ttatttagca cataaacgat acacagcttc
    34981 tgcataaatt tccattgctt tatacatact acttaattcc catcgttcat tcgcctggtg
    35041 cataaagtca ggagtatcag ggaacatcgc accgaatgcc acacactggt tcatcgtacg
    35101 agcaaaagta gcgccgcctg atacaaacgg ttcagtcatg tctccagtaa tatcgcgata
    35161 tgtccccaat agatttttca ctaactcact gtctagtggc acgtataatg aatcaaggaa
    35221 gtcaaattct tcataagtca acccatattt ttctgctgtt ttagttaatt tagctactaa
    35281 atcatctttc ttgaatgtaa cagggattct catatctaca ccgatttttg tttgttctgg
    35341 tgtgatttcc aaacttgcaa agttcatcgt tagctcgccg gattgttcat cttctgtttt
    35401 gcccacgaca gcttcaccag ttgcattttc ttgaactaat tttcctaaga aattgattgg
    35461 gccaaaatca aatacttctg aaagagcaat tgccagacga gaaatagcat tcactccttg
    35521 tgctgcatct ttcgcgtgga tacttttacc aagaacgacg atgccttcac cttgttcttc
    35581 gaaatcgaat tcgtgttttt tcaatgcttc ttttacttca gaaagctttt ctccagagta
    35641 aggagctgca tcaggaacaa cattcaacgc tcctccagct tttacagaaa actcgttcgt
    35701 tcctggtcct gtcaagtaag cttgcaataa gcctttttct gcataaatta aaggaaattc
    35761 agcatctggc gcaaatcctt gcgtaattcc ttcttctttt tcgttatatt tttctaaaca
    35821 acgccataaa ttttcttcat ctgtaccaaa gataaaacga atacgtgtct tgaattccac
    35881 gccagcatca atcaaagctt tgacggcata cattgccgca atagaaggac ctttgtcatc
    35941 ttgggatccg cgaccaacta accagccgtc ttttatagtt ggatcgaatg gttttgtttc
    36001 ccaattattt tcgtcccctg ctggaacaac gtccatatga cacaagatac cgaataattc
    36061 atctccagag ccaatctctg aataaccgta atagccttct ggatctttaa atgttctaaa
    36121 tcctagcttt tcactgattt ccaatacttt atccaaagct tcaatcactt ttttaccgaa
    36181 gggatgtcct tgtcctgaat ctgcttcatc tagtacggaa ggaattcgga tcaattcgct
    36241 caatgattct aatgcttgtt catgatgttg ttctgtcaca aattttttca tgatattatc
    36301 ctcctaatat gttaaaaagg gtcatgtcaa attcctccga cacaacctct tcttctcaaa
    36361 taatgacgtt ttaagcctgc tccatcagca agcagaatgt tcttaacaaa tgatgaaaga
    36421 aaaaacactc actgaatgga gctattgact tatccacgtg cttatcgata gccgtttatc
    36481 ggtttcttca ttcttatgat agaccttgta cctgtatttg tctaggttta gaacattgct
    36541 gctacaccta ggatcacaca gatggctaca aataatatcg caattaattt accggtaaat
    36601 ttccaccaca ctgaaacatc gaaacgagca attgctacag cacccattac gatagcagat
    36661 gttggtgtaa ttaagtttac taaaccagat gcagattgat aagcagtgat tactaaatcc
    36721 ttaccgacgc cggcaaattc acccataggt cccatgattc ccattgttgc agcagctaag
    36781 ccggaagttg aaggaatcaa gaatgacata gggatataga aaataaatgt aataatgatg
    36841 aatacacttg aagataatga acttaatcct tgttcgcccc aatgtaagat ggtgtctgtg
    36901 attaatccat tgttcataac cacttggata ccacgagcaa cagctactac gattgctaca
    36961 ccgatcaaat cagaagcacc agctaaaaat tcagagacaa attcagactc tttcatgcca
    37021 taagtgattg ctactacgat ggacattact aagaataaca tagtaatttc tgggaagtac
    37081 cagtcaccta atggaagcat gcttttacct agaaccgtac ctagtaccgg aaggtttgtt
    37141 aaccagtttg tcaatgattc aaaaatcgtg aaattcgcat tcaaggttgt ccaaggaatc
    37201 aaactgatga tcatgatacc aaaagtaata aagaacaata cattaacatg tttttgtttt
    37261 ttcgtgatta cttcttgacg accgattgct tcaatatcga aatgcttcat atcttcttta
    37321 cgttggttgt atactaatga ttttgttgga tctttttcga tctttgatgc ataacgatac
    37381 acatatacaa tagaaatagc taacaaaata acgaacatca agaatcgaag accaatacct
    37441 tcacccatcg aaatcccaac agcttgtgaa gctacgcctg ttgcaaaagg atttaccgtt
    37501 gaagctagac agcctacttg agaccctact aatacgattg ctacagctac tactgtatca
    37561 aagcctacag ccatcatgac tgggatcagt aaagcataga atggaatcgt ttcttctgcc
    37621 attccgtaag tagagccgcc taatgcaaat aacagcatca atactggaat aagcattttt
    37681 tcttttcctt tgaatttctt cacaactgaa gcaatcccgg catctagtgc ccctgttttg
    37741 ttgataatac ctaaaaatcc accaacaacc aaaatgaaga aagagattgg gattgcagca
    37801 ggtgtttctt tagttagctc ccccgctggt acacctagca tcccattgat tggcgccatg
    37861 aaaatatccc acagcccttg aggattttgt tgtactcgtt catatgatcc agcgacgatg
    37921 ttgcctgcat cgtctacact atagtgccca gctggtataa accaagtaag tacagcgatt
    37981 actgcaataa taataaataa gaccgtatac gctgaaggca tttgaaattt tttctttttt
    38041 gtttgttcca tcttcttctt cctttctttt ccgttagaga caacaatttt ggctctaatg
    38101 ttattttatc aaaaagagta tcagaacaaa aataataaca cgcattttgt aaaacttttg
    38161 ttcaaaaagt tcgtttttcc ctattccctc ctcttttttc aggtttttaa agctttcttc
    38221 tttttcattg tagcgtttcc ttttttttat aaacaaacat tttcgtcttt taaaaggaga
    38281 agaatttcac atttgcaaag aattgattga tttttatgca taaatcaaaa aaatactcaa
    38341 aacaaacaaa taaaacgttt acattattgc atgtcttgta tttttttcca aaaaaaaaga
    38401 ccaagaaatt tcttgatctt tttgatattt atttgactgc tgtatgacgt tgttcgaact
    38461 gatgttgttt tcgacttaac catggaagaa gttgcgcatg cagcactaaa aagacagcac
    38521 tcacgatgaa tcctttgatc aagttgaaag gaacgatccc aatcagtaca tagtttgcta
    38581 aagacatgcc cagcatctgt gttgcgttca gccctaaaaa cattaagtaa agaggcgtaa
    38641 taacgaaata atttgcgata ctcataaaaa tagttagagc gatggttcct gtaatgacac
    38701 ctaaaaattt gtttcttcct ttatgctgtt tctttacact aaagaaataa taaatcggga
    38761 atgtaaagat cgttgttgat aagaaactag cagtgtctcc taccaaatta tccggagaaa
    38821 aacctgtagt cagcagatgt aatactgaac gcaagaacgc tgttgcaact ccggccgctg
    38881 gaccgaataa gaacatgctg atcaataccg agatatcact aaaatcgatc ttcaaaaaac
    38941 taaaagccgg catgatcgga aaagcaaaat actgtaaaac taatgcgatc gcagcaaaca
    39001 tcgctactgc aaccattttc tgtacacggg tgttcttcat ttgaaatcct cctgttagga
    39061 ctcctcttca gcgaactttt tgacgtttct ctccaaatga aaaaactcta ttttccaggg
    39121 aaaatagagt tagagttatt tgatcaagcg tcgtcaaata agctctatct tctttatcca
    39181 gactatactg tcggtatcgg aatttcaccg attcagctat ttttttgcaa aataggtcgt
    39241 ggactataac caccggtcgg gaatttcacc ctgcccctga agacgaacct tatataattt
    39301 ttgtttacat attcattata catatctatt caaaaaacgc aacatctaac acttataaaa
    39361 agtaagtgtt cattttgagt ggttcagcaa actgctgact tcttttttgt tcagttcacg
    39421 gtaatcccca ggacgtaggc cttgtaaagt cagatctccg tatttttcac gttttagttt
    39481 ttgcacagga agaccaactg cttgaagcat atttttcact tgatgattac gtccttcgtg
    39541 gatcgtcaat tccacgatac tgtggtttgt cttaggatcc actgataaaa tttggaattt
    39601 agctggagat gtttttcgtc cttcgatctt tattcctttt gtcaaaggtg caagcatcga
    39661 ttttgtagcg atccctttta cctttgcaac gtaaacttta tccacttcat gttttggatg
    39721 tgtcaggcgc tgagcaaaat ctccatcatt tgtcagtagc aagatccccg aagtatcgta
    39781 atccagtcga cctacaggat agatacgttc ttttaccatc ggcaaaagat ccgtaacaac
    39841 ttttcttcct ttgtcatctg caacagcaga aataactcct ttaggtttgt acaataaata
    39901 gtaaccgtat tcttcttgat aaattgggac ttcatccact tccacagtgt catgtttccc
    39961 aacttttatc cctaattccc ttacaacttg tccgtttact tttacacgtc ctgcaaggat
    40021 gaattcttct gcttttcgac gagaagtgat gccagcgtgg gcaattactt tctgcaatct
    40081 ctccattaca attattctcc tttattcatt ccatcaaaga atagatctgg tgacacgtct
    40141 tcttcaagtg aaatttccat ttcttgtgta tcaggcagtt cttctaatga tttcaaacca
    40201 aaataatcta agaagtagcc agttgttcca tataatatcg gccgacctgg tccttctact
    40261 ctaccttttt cttcaatcag tttatgggca gctagacgct gtaccgctcc tgaaacctga
    40321 actcctcgaa tctgctcgat ttcagctctt gtgattggct gtttataagc gatgattgct
    40381 aaggtttcga cggcggcttg agataatgtg tttgatgtcc cttgcgcata tcgcttaagt
    40441 aagggagcca gctctttttt agtcgttaat acataatggc tttccgtttc aaaaatatgc
    40501 aaagctgagt gcgagttttg agtgtaagat tcttttaatt ctttcactaa acttgtgact
    40561 tctgcttcgc tgagatttaa cagtccgctt aattcttcat tactgatccc ttcctcgcca
    40621 acaataaaaa gaagggcttc aagttcactt aatttcgtca tgttcggtat ctcctactgc
    40681 tggataaagc ataattgatt catagttttc tttttgttgc gcaatgatct gccctttttt
    40741 catcaattct agcaaagcca taaaagtagt gacaacttca cttttagaag gtgactcaaa
    40801 aaaagaatcg aaagtacagc catgcgcatt tctttttata cgctcttcga taaataccat
    40861 tcgttcttcg atcgttgtgt catcgggcgt gatcgttttt tccacttttt gagaaaattt
    40921 cttacgttct aagattttct gcatggcacg gatcaaatcg atttttttga cttgtcctcg
    40981 cttcaacggc ttttcagcat ccgctaaccc atcgatgttc ataggttctt ttgtgtaata
    41041 aagacttcgt tcctctgctt tttctgtcag tctttcagct gcatatttat atttacggta
    41101 ttctaaaagc tgagtgacca attcttctcg aggatcttct tcccataatt cttcatcgtc
    41161 tgtgacgaat tcttggacag gaagcaatgt cttgcttttg attgccatca atgtagcagc
    41221 catcactaag tattctcctg caacagctag ctcgagtgtt ttcattgtat ggatataatt
    41281 catatattgc tccgtgatcg tagcgatcgg tatatcatat atatcgattt ccatttgctg
    41341 aatcagatga agcagcagat cgagaggacc ctcgaataca tctaatttaa tattgatctc
    41401 cgtcaactca ttcactcttt cttctttttc tttgctagcc attttccaat gtaaggctct
    41461 gtgatttcag ttgcacggat ggagacatcc gggtactttt cctgtaattc atctaacatt
    41521 tgaaagccca tattctcacc gcgataagac ggattcaaag aaatgtcatg gatgatcagc
    41581 tgatcgttcg ttgcccagta aataccaata atcccttgaa tgttggttga accttctggc
    41641 gtaaagaaaa aaagttcata atcgtcttca gattcgtacg tatcaatctc cttcaacaaa
    41701 gaagttttac ctgtcagttt cttatggaaa ctcaatagtc ccatagcaat tttacggttt
    41761 tctttccggt aatgtgttaa catcttctca ccttcctatg actaagccct tggaaaatgc
    41821 tgcttataaa catccgtcat tcttttcttc gtgatatgtg tataaatctg agtcgtagag
    41881 atatccgcgt gacctaacaa ttcctgtact gtccgcaaat cagctccatt ttctagcaga
    41941 tgtgtggcaa agctatgccg cagcgtatgt ggtgttactt ctttgtaaat cccagcctct
    42001 cgtactaact gcttcagatt tttccagatt ccttgccgag aaagaccttt cccatgatta
    42061 ttgacaaata catgcatttc agatgcgtcc tttacaagta atggacgtgc ttcgtctaag
    42121 tagcgttcca gccattggat cgcataatca cctaaaggaa tgatccgctc tttatctcct
    42181 tttccaactg tctgtaccag tccaagagaa agatgaagat ctcccaattt caatccaatc
    42241 aattcactga cgcgcatccc agtagcatac atgacttcca agattgcacg atctcttatc
    42301 cctaacgttt ttgtcgtatc aggtgcttca atcagctttt ctacttctgt cagagtcaat
    42361 gtacttggta atttctgtgc tttcttcggc gtatcgatat gttgcatcgg atcatgatca
    42421 gtgatacgct cttgccgtaa aaactgatgg aatcttctga gacttgagat catccgagta
    42481 acagttgctg tcgctttttt ttcttcatgc aacttctcca gaaaacggat gactaaaaag
    42541 cgatcgacat cctgccatga agcaattttc tgctcctgta gaaaatgcaa gtactgttcc
    42601 aaatcacgtt catagctttg tcgtgtattg gcggacaatc cccgttcaat attcaagtag
    42661 tggagataat cgttgatttg ctctttcata ataatccccc tcatgcctct ctatcatatc
    42721 agataccgct tcattctgat agtctttttc cataatctga gcaaagaaaa agccgtctag
    42781 acactcagac ggactggttt cttttttctt tttcatagca ttcctgacag attccatgga
    42841 acgttagccg atgatctttt actaaaaaat gataacgttc ttctactact tgctccactt
    42901 caagtaaaag atcctcttct acttcctcga tcgtgccgca gttcaagcat agtaaatgat
    42961 gatggaaatg tttggcgcct tcttttctca aatcataacg ggctaatcca tcattaaaac
    43021 ttactttatc gacaattttc aattctgtca agatttcaag ggttcgataa acagtagcaa
    43081 gtccaatttc cggactcttt tttttcacta aaaagaagat ttcttctgca gataaatgct
    43141 ctttctcatt ttccaaaagc acaagcagag tagcttctct ttgcggtgtc agcttaaacc
    43201 ccgcttcatg tagctgctgt tttgtttttt tcatcgccga aatagcattc atgaccattt
    43261 actcaacccc aacaatttat aataattaca aataaaaaaa gatataacac tatgttaatt
    43321 aaaattatta taagctagtt aagaaaaaca tgcaagggat tctcaattag attttttgac
    43381 tagttttgtc acaccgtttt cttgtgtaat caatccttgt ttcaaaagat tacctaatgc
    43441 gcgcttaaat gctcctttgc tgataccaaa agctttcatg atttcttctg gatccgattt
    43501 atctgtaaag ttgatttgct gatctgcaga acgttctaaa atggttaaaa tcatttgtgc
    43561 atcatctgat atagcttcat atgctcttgg cttcaatgaa agattcagta caccgtctgg
    43621 tcggacacca atcactcgac cttttatctg ctcgcctaga cgaggttcat tatatcgttc
    43681 agaaggatgg ataaacccaa gatagaaatc ttccgttaaa acgtacgtac ctgttaattt
    43741 caagcggtat gctgttccta tcacatcttt gtttttcatt tcttctgttc catatttgct
    43801 taaagatttg aatatttttt catctgctaa actgccccaa atacggtctt ttgcatcaac
    43861 tctaagggaa atcatcaagc gatccccttt tttaggccat agctctgtca ttgtcggcaa
    43921 ttcatcaaga gatacgacaa agtctttatc tggaagaccg atatcgacaa acgcacctaa
    43981 atcttttcgt gtagcagtaa cgattccaaa agcgtaatga ccgatacggc ttttaggaat
    44041 ttgtgtagtg atccgatttt catgtttttg attttggtaa ccgaatcctt caactgcttc
    44101 accgattttg tgttcacctt ctgatttgct taagccaaag gtttgtccgt ttttttggac
    44161 aaagtagttt ttttcatttt catcaataat aatacctgta aatatagttg ctaataaatc
    44221 gttcattttt ttctccctct atagtgaaag gttttcttaa tacatttctt tgctatcata
    44281 acataatgtt atctacttga atagctgagc cttggtttgg aaaggtgaaa aaaggagtat
    44341 gacaacagtt gtgtcatact cccctttttc gaataaacgg tgttaaacgg caggctcaca
    44401 gcctctttca tttgcctaaa attaattgct cccatttcct ccgaaccatc cagttttaca
    44461 gttgatggtt acgatgtaaa atggcaaagc actttctcca atgcctttca tcaatgcaga
    44521 ttttgttgaa gtggcatata gattataata gccttcccca ttttcgattc tggctttttc
    44581 taatctgtaa cctccttcaa cgatatatcc acgttggata catgtagcaa tcactttgct
    44641 ttggacacct ggtgcccacg tccatgctgg attagatgca tcggcaatat cacttgctct
    44701 ggcagatttg attggctgat tcgctgagtt tgaagaggaa ggggtcgagg atgccgcttc
    44761 ttgctgtgct gcttgtgcga ccgcagcttg ttgagcggct tgagctgcag ctgctgcttg
    44821 agcggcttgt tttgcttgtt cttcagctgc ttttttcgct tcttctgctt ttttcttttc
    44881 ctcttcttct tttttctctt tatcagtgag catcttatct acttgtttca attgttcatt
    44941 caactgagca gatagatctg tatttttcac tttttctact gcttcttttg ccgtcttgta
    45001 ttggtcgcgt gttgctgaat ccactacttt tccgtcttta aagactatag ctgttttttc
    45061 tttagctgtt tgcaattcag catattgatt tttagcttct gtttgtgctt gttgaatcaa
    45121 ttttccaaaa tctgtgttat ccgtttcgat tgcttggatc tctttatctg ctttcaactt
    45181 gatgtcatct gctagatgat catcgacgat ggcaggtgct atgaaccact cattcacttt
    45241 ttggatatct cggatcttag attggatatc ttggtaagtt gtttctaact ctgaatatcc
    45301 tttttcattc ttaagactgg ataaatcatt ttttagtttt gataaatctt gatgaatcat
    45361 atctgcacga ataaattgat ggtgatcatc tgtataaaag gcagcaagtt tctcatgaag
    45421 ttcttccatt tgttgctgtc tttttgcttc tgctgctact tcggcttcta ctttttgatg
    45481 atggtcataa taagcccagc ttcctccacc gagtagaagt actgctgcta aaacgagata
    45541 aggtactttt tttgattttt tcttttgcgc tttatctgga tctgtttttt ctaagtttga
    45601 ttcttgatgt gttttttcag tatctggcac gattgttatt gatttcgctt ctgcttcaaa
    45661 cagcttaggc tggttctctt ctttttctat ttttgcttca agatcagtac cctctttgct
    45721 tacagattga ttttcagcat cggattcact atttatttca tcgggctctg ttgacttttt
    45781 gtctgtgttt tcgagtattt cttttttctc tgcttccggc agtttattcg ttttttcctt
    45841 ttctaattgt ttttcagtaa atacttcagt tttttctgtg atatccgttt ttacgtcttt
    45901 tatttttact gtttctttac ctgtggcgtt ttctctatca gagtcttttt cagtttcttg
    45961 ttccttttct ggctctactt tgttttcctg tgcaattttt tcactgatca ttttgagcag
    46021 ctctttcgcg tgctcttctt ttgtatcttc acgatgtgct ttgatataag cggatagtat
    46081 cgaattttct tcctcattat cgacgatttc tttcagttca gctactcttc ttttttgttt
    46141 tgattcaagc gtttcttctt gattctttcc tgattcttcc gaagtctcat gaacagttgc
    46201 tttcttctgt tcatttttct ctatattagt tacttgatcg tgagcaggtg gttcttcgtc
    46261 ttctggttct gctttttcga ataattccat tacagattct agaggtaaat ctttcaattc
    46321 cgaccattcg attggatcgt ttaattctgt atcaggaaag actgcatctt cagttgattt
    46381 atcctgcata gctgcatctg tatttgtttc ttcaattgat tcttttattt gctgatcttc
    46441 tgcagttatt tgatgaccgc agtctggaca gatagtattc tgctccattt ttttcgatcc
    46501 gcaatttgga cattttttca tcacttagcg atcggctcct ttactaggtt tacgctacca
    46561 tttaatctat catattctat acgtcacgcc aatagcttat ttttaggatt tacttaaaaa
    46621 taatgaatga atttccttcc tatagaaaaa aaagcttgag actgcgtagt ctcaagcttt
    46681 ttttctttta aaataaacca aacagtttta ctgatttctc ttctggtcaa tagtccacgc
    46741 ttattctaag aaatctttta attgttttga acgagaagga tggcgcaatt ttcttaatgc
    46801 tttagcctcg atctgacgaa tacgttcccg agtaacacca aatactttcc ctacttcttc
    46861 caatgttctc gtccggccat catctaaacc aaaacgtaag cgaaggacat tctcttcccg
    46921 atcagtcaat gtatctagta catcttccaa ttgttctttt agtaactcat aagctgcgtg
    46981 ttctgctgga cttgttgctt cctggtcttc tatgaaatca cctaagtgag agtcatcttc
    47041 ttcaccgatt ggcgtttcaa gggaaacagg ttcttgcgcg attttcaaga tttctcggac
    47101 tttttctgtt ggaagatcca tttccgcacc gatttcttct ggcgtaggtt ctcttccaag
    47161 gtcttgcagc aattgacgtt ggatacgtat caatttatta attgtttcaa ccatatgaac
    47221 tggaatacgg atcgtacgtg cttggtcagc gattgctcga gtgatggctt gacgaatcca
    47281 ccatgtagca taggttgaaa acttaaatcc tttgcggtag tcaaattttt caactgcctt
    47341 catcagtccc atgtttcctt cttggatcaa gtcaaggaac tgcatgcctc gtcctacata
    47401 acgtttagcg atacttacta ctaaacgaag gtttgcttct gccagacgct gtttcgcttc
    47461 ttggtcgcct tcttcgattt tcaatgctaa cgctacttct tcttccgcag ttaaaaggga
    47521 gacacggcca atttctttca gatacatacg aaccgggtca ttgattttta caccggttgg
    47581 cgcagaaaga tcttctgttt ttgcttgttc agcttttttc tcagcactct tcaagctgtg
    47641 aattgatgga tcaccatttt catccaccac actgatacct gcgtcttcaa tcttttggat
    47701 caatttgtcc atttcatctg cattcaaagt gaatggtgta gcaagctgat tggataaatc
    47761 atcatataca acttgtcgtt tgggtttgtt ttctttgatg aaagctgcta ctgctgcttc
    47821 atacgttttt ttatctgttc catttgccat aaaagaaggc cccccttgag tagctatttg
    47881 gcttgtttta actgtttcgt taaattgatg atttcaatcg tcaattcaag ctcaagctgt
    47941 ttgtttccag ttcgtctggc ttcttgctgc atttgttttt ttaatgttat ttcatcagct
    48001 aagccagatt tgctgatcac atctagcaag tcttggattt ctcgtggcga actttcttct
    48061 gccaccggta aatattcgat ttccaccacc aaccgcctta aattttcatc ttgcaaaaag
    48121 tctaaaaact ctgcaaggtt gaattcataa tgttgtgaga catatgcatc gaataaaaaa
    48181 taaacttctg catatgcatc atgaataaac tgaaattctc gttcttttaa aatattacgg
    48241 acagaaactt ctttaaatgc acgatataat agaatctgct ctgccttttc tacttgcgtc
    48301 aaaggtctct ttttccgtgc agtttcttgt ctcgtaacct gagtgtgttg actttcctga
    48361 ggttcttgac gattttgctg atttgtcctt cgaagatcgc gcagttgttt ttgtatcgtc
    48421 tctctgccga tctgaaactc tgttgacagt tgagatagat acatatcttg ttcgattggt
    48481 gaaacaactc ggctaagttc aactagtaat tcttcgaggt attcgatctt gtctttttca
    48541 ttatctaaat ttttcccttg ttttaaataa cgtgatttaa aagtaaagac tgtttcgcgc
    48601 ccatgagcca ctaattcttg gaaagatgct tctccatact tacggagata ttcatcagga
    48661 tcaagctttt ctggaacgac tacgacagaa agtgctaatc gactgttttg ccgaagcaat
    48721 tcaatccctc gattggttgc ttcaaaacct gcgttgtctc cgtcatagca aaagaccaat
    48781 tctttcgtca gcttttccat ttgttggatc tgttgagctg ttagacttgt ccccatagaa
    48841 gctaatccag ttttgatacc agcctgccat gcagcaatca catccataaa cccttcgaac
    48901 aacaaaattt ctccgttttt ccggatatcg cttcttgctt tatcaagatt aaataaaact
    48961 tgtcttttat tgaacagttc tgtttctgga ctattcaagt atttgggctg atctttttcc
    49021 tgcccttttt caggtgcaag ccagcgtcct gaaaaaccga tcgtctggcc ttgagaatta
    49081 cgaagtggaa acatcactct ttgataaaaa cgatcagaca tacggccatc atcatgcgtg
    49141 acaaacaatc cagagcgttc taacaattca tttgaaattg attcattctt gaatacttgc
    49201 tgaagaaaag aacgctcatt aggtgcaaaa cctatgttga attcttcaat taattctaaa
    49261 gacaacccac gactttgcaa atattctaaa gctggctgtc cagcttttgt atgcaatagc
    49321 atgtgatgat agatttccgc tgctttttca tgagcagcaa tcagtttccc cgtagcagat
    49381 tcaggatttt ggacagctat agtacgctga cgccattgat cttctaacgg tatctgttcg
    49441 aagtctgcca cttttaccac tgcttctgga aaacttagac cttctagttc ttgaagaaaa
    49501 gtaaacacgt ttccgccttt accacaacca aagcaatgaa aaatctgctt gtcttctgca
    49561 acagaaaaag acggtgtacg ttcttcatga aaaggacaca gccccaagta atttttacca
    49621 gactttttca actggacata ttgtccaatc acctcaacga tattcgtttg actgcgtact
    49681 gtttcgataa cctcttgagg aatccgttga gccatttttt caccccctgc tctattgata
    49741 tcttttgggt ttaacagaag atatttcagg agaataaccg gtaaaaaatc gcactaactg
    49801 aaatcagtgc gattttaaag gacacaatta tacatgtttg attgtatcat atttcattgc
    49861 tttttcaagt ataacacatt tctatctcat taagcatcaa tttgctactc ttttcagtaa
    49921 ctgattttga atatcagcct tccagtacaa tactcgaaga acaatattta cgagcatgaa
    49981 tacaaagaag tatcctacgc tctctcccaa ccctaagttt acttccaaaa ttccatggaa
    50041 cagcattgca gctacatata tccctacgat cgtcgttact gctaggaaga tgcgcaatgg
    50101 gttgaaggga taacaagctt ttacaactgc catgcaactg atcccaatca gcaagtaata
    50161 catcaaagta gtagtttcag catttcccca tgcgaatgtt tttccaagta gatacaccgc
    50221 aatgatattc actactacaa gcaaggcatt tggtaaagca ttaataagtg ctgtaggcaa
    50281 ataacggtaa gtgatttttc ttttatcttg ttcaaaagat aaaaagaagg aaggataccc
    50341 ttcaatcgct aaatcaatca gcgtgatttg gatagggata aacggaaatc caattgctgt
    50401 aacggcacat ataatcgata aaataaatga ataaatcgtt ttgataaaaa agatgcctga
    50461 gactttcgtc acattgttga ccactcggcg tccttcaaac aggacttctg gcaatgtagt
    50521 aaaatcagag tccaacaata ccagattcgc aatctgacgt gttgctccat caccttctgc
    50581 catagcaatg ctgcaatctg cttctcttaa cgccaaaaca tcattcacac cgtcgcctgt
    50641 cattgcaacc gttctgccgc tttctttcaa ttcatttacc agcaatttct tttgctgcgg
    50701 actcactcgt ccaaaaaccg tatagcgatg agcagcttct cgcacttcca tttcttcagt
    50761 aatttgagaa agatcgacat acgcatcata attcggtaat cctgcacgtc tagcgatatt
    50821 ggagacagta aaaggattgt ctccagaaat cactttcaga tccacacctt cctcatgaag
    50881 ataagctagt gtttcttgag cgttttttcg gattggatca tcgatttcaa acagagcaac
    50941 cggctcaaca aatgctggtt tttcttcgtt caattgctga tttggagcga tccccaacat
    51001 aagtacgcga tagccattcg cttgggctgt ttttagactg tccggaagcc gatcttcttc
    51061 caccaaacgt tcaggcgctc ctacgtacac gactcccagt tctggaaatt ccatcgcgcc
    51121 ccatttgcga tcagaagaaa atgcgacttt gtttgtcaca tcataccgat tagaaagctc
    51181 aaaataatct ctcatagccc gcatagtgat gttgttatca ctgctttctg ccagatacgt
    51241 ccccataatt tctgggatgg ctttctcgta cagcggatgt agtgcttcta cccgctggat
    51301 actcattttc ccttcagtaa tggtccctgt tttatccaag cataatgtat cgacatgggc
    51361 cagcgtttca attgaataca tgtcttgaac aaggattcgt ttttttgcca gtttggttac
    51421 ccctgtcgtt aatgcaatac ttattagcaa taccaaaccc tttggaagca tgcctaacaa
    51481 agctgctgca gatgcaataa cagaaatttt gatagaagca tcgcggatga acagtgcttc
    51541 caaaaatagt atgatcccca aaggaataat cacgaaacta gtgaatttgg atactttgcg
    51601 aatcgaatct acaagttcag aacgaatagc tttgtgagtc tttgcctcgc tagtcaagcg
    51661 gacagcataa ttatctgcac ctactcggat aacttcagcc atgacagaac cgcttgtcaa
    51721 aaagcttcca gaaagcaatt cagctccagt ttctttgaca atcaaatcag attcgcctgt
    51781 caaaagagct tcatttgctt ctgcccgtcc agatataacc tgtaaatcgg ctggtatctg
    51841 ttctcctgct ccgattttta ccaaatctcc taataccagc tctttagtag aaatcgattg
    51901 ttctttaccg tcccggatca ctagtacttc tgattgggaa acaatcgata atttccgtac
    51961 aagatttctt gcatgtattt cttgataaat tccaataacg atattgacga caataattgc
    52021 taaatacgcc atgttggaat acgcacctac aaataacaga caaatcccaa taccaaagtt
    52081 cagaaaattg aataacgtca atatattatc gctgaatatt tgttttgtac tttttgatgc
    52141 atcttcctgg tagtcatttt gctggccaga agccattttc tgaactacct cttgctgtgt
    52201 aagtccattc attttctttt tctccatttt ccgccaactt tcaaaaataa tcacgtgttt
    52261 aagtctagcg tgttccaaga ttaaaaacaa cttgttttta taaaatttat agaaaaacac
    52321 acgaatgatt ttacaaaaaa gatgccaacc tttttgtttg aatcacccgt gtgctgtttt
    52381 tattatcaat ctatctttac ttatattgtt cctccaaacg gctcatccac caacctagtg
    52441 ctgtaccggc tataaaaaga acaggacaag ctagtacccc agcaactccc aatcccgcgt
    52501 aatcggtagg aatgatcata atcgttgaag caaaaactac acctaaaatg aaatggaata
    52561 gcttgctata tgcctttgag aaaatgtaat ccatgatttt tgacaatcca agaacacaaa
    52621 gaatcccacc aattccgatc ggcacaatca cttcaagtcg tacttcttta aatccgtcag
    52681 ccatcgcttt atacaatccc atatacacca aaaaattaga agggctcaat cctgggacga
    52741 tcatacccaa accaatcaat ccgccggcaa tcagccatgt tccaaaattc tgatcgactt
    52801 gcccgtcaaa taattgagcg ccgtataata aaaacaaata agccagaaca aaagtaacaa
    52861 ccatgatgat cacatcagaa gtatctcttc cttttttccc tgcttctttc cataacgcag
    52921 gaacagttcc agcgatacat ccgacaaaaa accataatat cgttgtttca tacgcaccta
    52981 ataaaaaact gactaaaaaa gaaagtagaa agatgcctgt cactccgcct agaccaacag
    53041 gcagaaaaaa caaaacattt tctttgaaat tttttgtaag gtgggctaaa aaagaaatca
    53101 gccgctcata catgccaaag acagctgcta atgcaccacc gctgactcct ggcaagataa
    53161 atcctgaacc gataaacatt cccttcacaa accgtaacat ccagtctttg gttgtgtacc
    53221 ccacttcttg attttccatt ttttgccttt tttccactat ttgcgtactc cttctttcat
    53281 ttaattttat tcatgattga acatttcctg ctattttact taaagacttt ttcataggtc
    53341 tctattattt tttcattcat tttgacaata tcaggataaa acatgttacc tccattatga
    53401 tagagatagc cgccattgta aagcaaggac tggatccgcc aatatcggta ttgtttccct
    53461 tcctcatttc ctaaaagtgg gctcagcaca tctttagaat acgcttctgc taattctact
    53521 gtgttttctc cgccatgatt ttttacatag gcaatataat ctttcccaaa attatatgct
    53581 tggacagctg tccataaatc gcatccttgt ttctttgctt ctactatcat ttccgccaaa
    53641 tatttgacac cttgatcaat actgtcagct ggattatcaa tgatctcagc ttctccatgc
    53701 agactttccg agctctgcat gagatctgtc ccttttcctt ttgattcggt caatattatc
    53761 gccttgatca aattctcgta gttttccata tgataatttt ttgcagctgt tgttatttct
    53821 atttgatagc tttccactcg ttttaccgaa cgataagttc ttcctcataa aaacaacacc
    53881 gcaccaataa ccgtcagaat gatcatgtaa aaaagaatac ggataataga taatcgtttt
    53941 cgcttcattt catcacctag ttattttgac atcttttttt atcttactat ttctttatct
    54001 tcttggctat ttcttccttg atttctgtgt acgccttaag aaaaaatgaa ttttcctgta
    54061 ttttttgaca tcttacgaaa atgatcgttg tttttcagta aaaacaattg cccaatgact
    54121 gatataatcg ttatacttta cacagtaggt gattatgtga aaaaagaaaa gaaatatgca
    54181 aaaatgccag atgggagcga aatttactat gaaaagagcg gtcaaggctt ccctctcttt
    54241 ttactgcatg gcaatgacgg cagcggccgc tttttttcag aacaggtccc tgtactcgaa
    54301 cgctactata ccgtgtatct agtagatagc cgaggacatg gtcgttcgac aaacgaagca
    54361 tctatgttga attttcagtt aatggccgag gatctgaata cgatcatgtt gctggaaaaa
    54421 atcgatcagg cagattttct tggatttagt gatggagcaa atctagcact ggtatttgcc
    54481 agcagcttcc ctaaaaaagt tcatcgattg attttaaatt ctggaaacac gcttgttaaa
    54541 ggcgttcgtt tttctgccag agtcatcagt aatattcatt atgcatgggt atggttactc
    54601 tctttatttc gcccaagtct tcgcaaaaat cttttagtta tcaaactttt gctgcatgac
    54661 atcgggctta cagaaaatga tttaaaaaaa atcaactccc ccactttgat catcgtggga
    54721 aaaaaagatg taatcaagct aaaacattct ttgtatatag ctaaaactat accaaaagct
    54781 tcctttgtgc ttgtgaaaga acaaggacat gaattagcta gaaaagatcc tgaacgcttc
    54841 aatcgagaag ttcttcaatt tctttctgaa acataactat taggaataga aggtgattta
    54901 tttgttaaaa aataccatac aatggctgaa aaaccaccta ggactgttca aaacaatctt
    54961 tttgatttct gtcattgtca ttattgtcgg tgaattgatg tccatcggta aaactctatc
    55021 tatccagcaa ttaggagaaa cgtttgccac tatacctgta tggaaaagcg gactaatgct
    55081 attgattggt cttatctctg tgttacctat ggttggttat gatattattt tgaatcggat
    55141 gcttgaacaa aagcagaatc ctcgttacct gtttgagaca agctggctga tcaatacgat
    55201 caacaatatt gccggtttcg gcgggtttgt tagtgtgggg cttcgctctg aactatatgg
    55261 acaaaagaaa gagagcagaa acgtgatcca agcattatcg aaagttttct tgtttttgat
    55321 ggctggactt tctatttaca gtctgctttc ttttttactt gtagtcttca cgccagttgc
    55381 tccttttttg aagcaatatt ggatatggtt gatcggcggc ggtctatatt ttcctgtcgt
    55441 ctttttattt actactttca aaaagaaagg attccttggt ggtacctcac ttaaaactca
    55501 aggacagcta ttgctagttt cattgttgga atggaccggt gtcctgttta gtttcctttc
    55561 tgttggttat ttaatggaaa tccggattga tctgtggcaa acaattccat tattcattgc
    55621 tgcttcagtg ataggaatcg tgtctatgat tccaggagaa attgggagtt tcgatgtaat
    55681 gatgattatc gggttgtccg ctattggtgt gccaagagaa atagtcgttg tgtggatctt
    55741 gctctatcgc ctgttctact acatcattcc gtttttgatt ggaatcgtct ttttcttcaa
    55801 aaatatcggt tccacttttg atcagcgtta ttctggtatc cctaaacaat tagcaacaga
    55861 aattgcgcat aaaatcgtgg ttgtcttatt atacttttct ggaatcatgc tagtactttc
    55921 tgccactatc ccgcaagctt ttacagagtt tcgctggctt catagtttga acccattgaa
    55981 atttcatttt attatccaat ttcctagtat actattggga tttcttttaa tcattatggg
    56041 cagagggatc gcagcacgtg tgaaacgagc atatttgcct acaatcttct tgattgttct
    56101 agctttactt tatgttttat taagtgattt tagtttcaca ccggtggttt tcttgtctat
    56161 tctgcttctg atcattcttg cttctaaaaa cgaattattt cgggaacaat tgatctattc
    56221 atgggagtgg cgtacgatcg atggaatcat tattggtgca ctgtccttgc tttacatcgt
    56281 tattggggta tacaatctgc cggatttccc tcatcggcgt caccatttta tttccttttt
    56341 cttatttcct tctgaaaaga tttggttttc gggattgttg gccatcatcg ctgttagttt
    56401 catgatcgtt ctatttgttc atttccttca aggagaaaag aaacagattg gcgaagcctt
    56461 taatgaagaa aaggccctga agatccttac aacttatgga ggaaactccg atagtcaatt
    56521 gatcttctta aaagataagc ggatgtttgc ctatgaaaaa gacggtgaac caactgttct
    56581 tctccaattc gcttgcttca ataataaatg tattgtaatg ggtgatcctt ctggtaagaa
    56641 agaagatttt ccagaagtaa tcgaagcttt tatcgaagaa acagatcgac tatgctattt
    56701 accagttttc tacgaaacaa gcgaagagat tgttatgatt ttgcatgaat tcggctacga
    56761 tttcattaag atgggggaag aagcttatgt agacctgaat agttttacaa cttcagggaa
    56821 aaaaatgaag ggcactcgtg ctgtcttgaa tcggatcgaa cgagaaggat ttacatttga
    56881 tgtcttacaa ccaccatttt ctactgagca aatgagtact ttcaaaaata tctctgataa
    56941 ttggttgggt tcacgaaagg aaaaagggtt ttctttaggc tttttctctg aagattatct
    57001 acaaagagcg cctattgcag ttgtcaaaaa tacccaagaa gaaattgtcg cttttgcaac
    57061 gatcatgcct acttacacga acaataaagt aggaacgatt gatctgatgc gatatgattc
    57121 taaaaaagca ccttctggca gtatggattt cttgttcttg catttgtttg cctatatgaa
    57181 agaagaacat atccaatggt tcaatttagg gatggcccct ctttcaaatg tcggaacttc
    57241 cagaaagagt tttttacagg aaaggattgc atacttggtt tacgagtttg gttctcattt
    57301 ttattctttc catggattaa gagaatataa aagcaagtac gctacaaact gggtctcacg
    57361 ttatactcta tattctagag atagttggat tgcatatgtt atgattgctt tgctgattat
    57421 tgataatgcg ccagttgagc agacacaagg tagaataagt ggtttgaaaa aatggatacg
    57481 aagaaagtat taaaaactgc ataccgatag atgaacactc tcatgatgaa taaaagagac
    57541 tgaagtaaaa cgaaagttca tgcttctaca atctcttctt atacaattga agaacaaaga
    57601 gcggtccaaa cagaaatcct gtttggaccg ctcttttatt ttaaacattt ttcattacca
    57661 aaatattcga gtcgcgtttc ttatgcttcg atctctgtaa cgatacctga acctactgtc
    57721 cgtccgcctt cacgaattga gaatgtcgtt ccgttttcaa cagcgattgg atggatcaag
    57781 tcaacatcaa tcgtcacgtt gtcaccaggc atgaccattt ctgtatcttc tggcaatgtg
    57841 attgttcctg taacatcagt tgtgcggaag tagaactgtg gacgatagtt gttgaagaat
    57901 ggagtatgac gtccaccttc ttcttttgtc aacacgtaca cttctgcttt gaattttgta
    57961 tgtggagtaa tggaacctgg tttagcaagt acttgtccac gttcgatatc gtcacgttgg
    58021 atcccccgta acaacacgcc tacgttatcc ccagcttccc cgtaatctaa cgttttacgg
    58081 aacatttcta cacctgttac aactgctttt tgtgtttcag gtttgatccc tacgatctct
    58141 acctcatcac cgacacgaac agcaccacga tcaatacgac ctgaggcaac agttcctcga
    58201 cctgtaattg agaagacgtc ttccactggc aatagtaatg gtttatctgt atcacgttct
    58261 ggtgttggga tatattcatc tactgtatcc atcaattcca taatagcagc ttcagcatct
    58321 ggatcgcctt gcaatgcttt taatgcggaa cctttgatca caggagtgtc atcgcctggg
    58381 aaaccatatt cgctcaataa ctcgcgaact tccatttcta ccaaatcgat caattcttca
    58441 tcatcgacta aatcaacttt gttcaagaaa acaatcaggt attttacacc aacttggcgt
    58501 gacaataaaa tgtgttcacg tgtttgaggc atcggaccgt cagttgcaga aacaactaag
    58561 attgcaccat ccatttgtgc tgcccctgta atcatgtttt tcacatagtc cgcgtgtcct
    58621 ggtgcatcaa tatgcgcgta gtggcgtttt tctgtttcat attctacgtg tgcggtattg
    58681 atcgtaatcc cacgttcacg ttcttctgga gctgcatcaa tactagcata gtcttgagga
    58741 tttgctaggc cttttttgcc taatactgta gtgatcgcag cagttaaagt agttttccca
    58801 tggtcaacgt gtccaatcgt accaatgtta acgtgtggtt tacttctgtc ataatgttct
    58861 tttgccataa tttacaaacc tcctaaataa tatagatcac tgaaaaattt cagctctcac
    58921 ctttttatta tagtcttttt ttgactaaat caaaaagaat acgtccgact tactttttgt
    58981 aagattcgaa ctaagcttat tatacgtcaa tattggtcaa agtcaactaa gagcactcgt
    59041 ctgtcttata cgcgaaaaaa gtacctttta taaataaaaa aatgtgtttt ttgagaaaaa
    59101 cttcaagata gtccttaact ttcttttgaa gcaataagaa caaaatgagg aaaagtttaa
    59161 aattcagctg ttgccaacaa ctttcaatag acgaaatctt ctatttcgtt aataataagg
    59221 aaagaggtga agtacatgaa aaaattaatt ggcgtacttg tcgttttagg catatttctc
    59281 ggcggaagtt ttgcagctta tcattatttt tacggcggcg aagcttatta tacaaaaatc
    59341 acgacgaatg gcgaaatgtc agctacccaa gcagataacg gagagaaatt cacgacttat
    59401 acctacaagc aagcggctta taataaagat ggtgaaaaaa aacaggtgac gcttcgcgaa
    59461 gaaagagaac atccattaaa gatgaatgcg tatcttaaac tcaaagtcaa tcctcgtaaa
    59521 ggtgtattaa gctgggaaga aataaaggct tctgaagtgc ctaaaaatgc ggcagaaaaa
    59581 atcgataatt agtaaatggt cttaaaattt tattagataa aaagaggctg ggacagaagc
    59641 gtttaactcc aagaaataag aaggagttgc ttctgcttcc gccgtttata cgtgtttaga
    59701 gcgtgagaca aaagtgttct ttacttttgt ctcacgctct ttttttgtta tattcgtctt
    59761 ttccgatcct tctcttgaca caattcattt aactgatcac tggtacttga acacctaata
    59821 ttctagtcat ttttataaac gattcaatac ttcatccaca tttttgatca tcacggatat
    59881 cgtaccatcc ggattattca caaattcaat caaatctggg ttgcgatata catctactgg
    59941 tacgatcagt tcgatcccgt ttgatagctt gaatttttgt tttccaaatt ttttctcaga
    60001 aatttcttgt acttctttta caggaggtgc ttctgggaca aatccttgct cttgtacttc
    60061 ttctttaaaa gccatttttg ccgtgatgtt ttccttgaag actttttccg caatcatctc
    60121 atgatccaac cgaccttttt cttctatcgt atcatagaca gcttctttca aatcagccat
    60181 gatatcaaat tcttctgtct caaatttttt tccaagatgc ttggctactt tttggatcac
    60241 ttttacattt tcttctaaag aaggtgcagg aacactttcg atcactcttc cagaaaaata
    60301 aagctctttt ttgccagaaa actcatattt tttttcaatt aattcgtagg tccaatcagt
    60361 caaattcaca gcgaatgctt catctgcttt ttgtgttttc gcccctagaa ttgctcgatt
    60421 caaaatcaag cggttgtcga tccctgcttc gtctgcttca acaaagtgag tgtaagcctc
    60481 acgataattg actttcaata aagataagta catcactgta tctaattcat acaagacaac
    60541 aaatacatct gcactaggtg cttcttcact ttcttggtag atatcgaacc accgctgaac
    60601 gattgcttca cttgcttcca caaaattttc ttgagcgatt ttaagtgatt caacgatcat
    60661 gctttcttct gctagttgtc cagtttttgt ttgagcagaa gagatttttt ggatcttctt
    60721 ttgaagaaaa tcacgaatat attcttttgt cagatccagc tctttttgtg aaaagactgg
    60781 agaaccagtt tctctgtcaa taatatgcag aattgctttt tttaaataga tatccatttt
    60841 ctcattccct tctgtcttag tttacggaaa aaaaatcaaa aataccagaa tcaaaatcat
    60901 tttcttttaa acaaaaattt caagagtaga aacaaaatgg attctttttt tcgaaaaatg
    60961 cgctacactt atagttgaac gaataaaaaa ggagctgaaa gtatgaattc attaacaagc
    61021 acttatcgtc tatcaaatgg ttacgaaatc ccggtagtag gattcggcac atggcaaaca
    61081 cctgatgggg atgtcgctgt ttcctctgtt aaagaagcgc tggctgcagg ttatcgccat
    61141 atcgatactg cccaaggata caagaatgaa gaaagcgtcg gccaagcgat taaggaaagt
    61201 ggaattccta gagaagagat attcttgaca acaaaattat ggaatgcaaa ccacagctat
    61261 gaacttgtga tgtcttcttt tgaagaatca ttgaagaagc tccaaacaga ttatttagat
    61321 ctgtttttga tccactggcc aaaccctgtt gcttttcgtg ataattggga acaagcaaat
    61381 gcagatacat ggcgagcatt tgaagaactg tatgaagcag gtaaaatcaa agcaatcggt
    61441 gtgagcaatt tcttacccca ccatctagat acactagcaa aaacagcaaa aattatgccg
    61501 atggtcaatc aagttttcct tgcaccaggc gaattacaga caactgtagt tgagtatgca
    61561 aaaaaacatg atatgatcct cgaagcatat agtccattag gcactggtaa aatcttcgat
    61621 gtgcctgaaa tgaaacaaat cgctgaagca catgacaaga cgattgcaca agttgctttg
    61681 cgttggtcgc tgcaacacga attccttcct cttcctaagt cagtgacacc aagcagaatc
    61741 aaagaaaata ctgaattatt tgattttgaa ttaacagaag aagaaatgaa acaaatcgat
    61801 cagttagatg gtgtagtcgg caaagcaaaa gacccagata ctacccaatt ttaagtcgaa
    61861 aggtgatttt atggatccaa tcgaacgctt gaattctctc tcggaagaag ttacccagac
    61921 ttttcattcc gattttgtct ttttgatcga tgcagagaaa atccaacatt ttcctgcaag
    61981 aaactggaca catgatcaaa tcatcgaaga actgaaaaaa cggttcgatc attcgctaat
    62041 ggtcaaaccc tggcacgaac atgaagtcat ttattcacct gaactacctg tttttgcctt
    62101 gataccgaaa aaatagaaag aa
//
LOCUS       DAYYHS010000005        61712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000005 DAYYHS010000000
VERSION     DAYYHS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.005"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHA6A_000277"
     CDS             complement(132..509)
                     /locus_tag="ACHA6A_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968517.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHA6A_000278"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHA6A_000278"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8968518.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHA6A_000279"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHA6A_000279"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8968519.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA6A_000280"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA6A_000280"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8968520.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHA6A_000281"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHA6A_000281"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8968521.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHA6A_000282"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHA6A_000282"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8968522.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHA6A_000283"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHA6A_000283"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8968523.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHA6A_000284"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHA6A_000284"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8968524.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHA6A_000285"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHA6A_000285"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8968525.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHA6A_000286"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHA6A_000286"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8968526.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHA6A_000287"
     CDS             10661..11575
                     /locus_tag="ACHA6A_000287"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8968527.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHA6A_000288"
     CDS             complement(11708..13273)
                     /locus_tag="ACHA6A_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8968528.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHA6A_000289"
     CDS             complement(13266..14717)
                     /locus_tag="ACHA6A_000289"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8968529.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHA6A_000290"
     CDS             complement(14731..15747)
                     /locus_tag="ACHA6A_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8968530.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHA6A_000291"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHA6A_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8968531.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHA6A_000292"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHA6A_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8968532.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHA6A_000293"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHA6A_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8968533.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHA6A_000294"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHA6A_000294"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8968534.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHA6A_000295"
     CDS             21340..22764
                     /locus_tag="ACHA6A_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8968535.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHA6A_000296"
     CDS             complement(22806..23063)
                     /locus_tag="ACHA6A_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968536.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHA6A_000297"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHA6A_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8968537.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHA6A_000298"
     CDS             24337..25314
                     /locus_tag="ACHA6A_000298"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8968538.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACHA6A_000299"
     CDS             25339..26982
                     /locus_tag="ACHA6A_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8968539.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHA6A_000300"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHA6A_000300"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8968540.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="ACHA6A_000301"
     CDS             28558..28833
                     /locus_tag="ACHA6A_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8968541.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="ACHA6A_000302"
     CDS             28839..29606
                     /locus_tag="ACHA6A_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8968542.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="ACHA6A_000303"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="ACHA6A_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8968543.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33636
                     /gene="recQ"
                     /locus_tag="ACHA6A_000304"
     CDS             31864..33636
                     /gene="recQ"
                     /locus_tag="ACHA6A_000304"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8968544.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33750..34052
                     /locus_tag="ACHA6A_000305"
     CDS             33750..34052
                     /locus_tag="ACHA6A_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8968545.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34055..35218
                     /locus_tag="ACHA6A_000306"
     CDS             34055..35218
                     /locus_tag="ACHA6A_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8968546.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35244..38672
                     /locus_tag="ACHA6A_000307"
     CDS             35244..38672
                     /locus_tag="ACHA6A_000307"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8968547.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38693..39817
                     /locus_tag="ACHA6A_000308"
     CDS             38693..39817
                     /locus_tag="ACHA6A_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8968548.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39798..40244
                     /locus_tag="ACHA6A_000309"
     CDS             39798..40244
                     /locus_tag="ACHA6A_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8968549.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40228..40566
                     /locus_tag="ACHA6A_000310"
     CDS             40228..40566
                     /locus_tag="ACHA6A_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8968550.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHA6A_000311"
     CDS             40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHA6A_000311"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8968551.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41242..41733
                     /gene="coaD"
                     /locus_tag="ACHA6A_000312"
     CDS             41242..41733
                     /gene="coaD"
                     /locus_tag="ACHA6A_000312"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8968552.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41723..42766
                     /locus_tag="ACHA6A_000313"
     CDS             41723..42766
                     /locus_tag="ACHA6A_000313"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8968553.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42925..43653
                     /locus_tag="ACHA6A_000314"
     CDS             42925..43653
                     /locus_tag="ACHA6A_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8968554.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43769..44014
                     /locus_tag="ACHA6A_000315"
     CDS             43769..44014
                     /locus_tag="ACHA6A_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8968555.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44011..44229
                     /locus_tag="ACHA6A_000316"
     CDS             44011..44229
                     /locus_tag="ACHA6A_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8968556.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44365..44904
                     /locus_tag="ACHA6A_000317"
     CDS             44365..44904
                     /locus_tag="ACHA6A_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8968557.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44897..47170
                     /locus_tag="ACHA6A_000318"
     CDS             44897..47170
                     /locus_tag="ACHA6A_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8968558.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47208..48254
                     /gene="holA"
                     /locus_tag="ACHA6A_000319"
     CDS             47208..48254
                     /gene="holA"
                     /locus_tag="ACHA6A_000319"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8968559.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHA6A_000320"
     CDS             complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHA6A_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8968560.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48997..49266)
                     /locus_tag="ACHA6A_000321"
     CDS             complement(48997..49266)
                     /locus_tag="ACHA6A_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968561.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49528..49656)
                     /locus_tag="ACHA6A_000322"
     CDS             complement(49528..49656)
                     /locus_tag="ACHA6A_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968562.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49736..50191)
                     /locus_tag="ACHA6A_000323"
     CDS             complement(49736..50191)
                     /locus_tag="ACHA6A_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8968563.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50329..52257)
                     /locus_tag="ACHA6A_000324"
     CDS             complement(50329..52257)
                     /locus_tag="ACHA6A_000324"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8968564.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52454..53287)
                     /locus_tag="ACHA6A_000325"
     CDS             complement(52454..53287)
                     /locus_tag="ACHA6A_000325"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8968565.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53479..54183)
                     /locus_tag="ACHA6A_000326"
     CDS             complement(53479..54183)
                     /locus_tag="ACHA6A_000326"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8968566.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54206..54832)
                     /locus_tag="ACHA6A_000327"
     CDS             complement(54206..54832)
                     /locus_tag="ACHA6A_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8968567.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55158..57533
                     /locus_tag="ACHA6A_000328"
     CDS             55158..57533
                     /locus_tag="ACHA6A_000328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8968568.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57557..58048)
                     /locus_tag="ACHA6A_000329"
     CDS             complement(57557..58048)
                     /locus_tag="ACHA6A_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8968569.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58235..59155
                     /locus_tag="ACHA6A_000330"
     CDS             58235..59155
                     /locus_tag="ACHA6A_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8968570.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59309..59653
                     /locus_tag="ACHA6A_000331"
     CDS             59309..59653
                     /locus_tag="ACHA6A_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8968571.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59843..60343)
                     /locus_tag="ACHA6A_000332"
     CDS             complement(59843..60343)
                     /locus_tag="ACHA6A_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8968572.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60871..60954
                     /locus_tag="ACHA6A_000333"
     tRNA            60871..60954
                     /locus_tag="ACHA6A_000333"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60905..60907,aa:Leu,seq:cag)
     gene            complement(61278..>61712)
                     /locus_tag="ACHA6A_000334"
     CDS             complement(61278..>61712)
                     /locus_tag="ACHA6A_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8968573.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20241 a        10663 c        13217 g        17591 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32881 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32941 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    33001 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33061 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33121 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33181 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33241 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33301 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33361 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33421 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33481 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33541 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33601 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33661 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33721 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33781 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33841 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33901 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33961 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    34021 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34081 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34141 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34201 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34261 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34321 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34381 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34441 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34501 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34561 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34621 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34681 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34741 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34801 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34861 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34921 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34981 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    35041 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35101 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35161 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35221 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35281 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35341 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35401 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35461 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35521 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35581 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35641 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35701 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35761 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35821 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35881 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35941 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    36001 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36061 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36121 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36181 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36241 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36301 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36361 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36421 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36481 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36541 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36601 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36661 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36721 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36781 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36841 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36901 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36961 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    37021 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37081 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37141 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37201 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37261 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37321 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37381 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37441 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37501 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37561 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37621 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37681 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37741 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37801 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37861 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37921 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37981 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    38041 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38101 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38161 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38221 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38281 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38341 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38401 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38461 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38521 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38581 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38641 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38701 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38761 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38821 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38881 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38941 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    39001 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39061 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39121 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39181 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39241 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39301 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39361 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39421 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39481 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39541 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39601 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39661 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39721 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39781 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39841 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39901 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39961 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    40021 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40081 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40141 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40201 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40261 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40321 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40381 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40441 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40501 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40561 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40621 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40681 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40741 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40801 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40861 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40921 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40981 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    41041 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41101 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41161 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41221 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41281 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41341 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41401 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41461 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41521 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41581 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41641 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41701 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41761 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41821 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41881 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41941 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    42001 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42061 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42121 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42181 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42241 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42301 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42361 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42421 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42481 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42541 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42601 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42661 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42721 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42781 agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42841 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42901 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42961 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    43021 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43081 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43141 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43201 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43261 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43321 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43381 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43441 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43501 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43561 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43621 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43681 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43741 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43801 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43861 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43921 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43981 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    44041 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44101 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    44161 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    44221 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    44281 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    44341 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    44401 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    44461 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    44521 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    44581 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    44641 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    44701 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    44761 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    44821 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    44881 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    44941 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    45001 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    45061 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    45121 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    45181 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    45241 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    45301 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    45361 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    45421 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    45481 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    45541 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    45601 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    45661 tttgtttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    45721 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    45781 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    45841 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    45901 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    45961 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    46021 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    46081 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    46141 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    46201 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    46261 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    46321 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    46381 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    46441 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    46501 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    46561 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    46621 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    46681 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    46741 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    46801 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    46861 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    46921 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    46981 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    47041 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    47101 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    47161 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    47221 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    47281 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    47341 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    47401 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    47461 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    47521 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    47581 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    47641 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    47701 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    47761 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    47821 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    47881 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    47941 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    48001 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    48061 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    48121 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    48181 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    48241 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    48301 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    48361 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    48421 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    48481 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    48541 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    48601 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    48661 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    48721 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    48781 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    48841 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    48901 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    48961 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    49021 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    49081 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    49141 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    49201 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    49261 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    49321 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    49381 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    49441 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    49501 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    49561 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    49621 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    49681 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    49741 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    49801 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    49861 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    49921 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    49981 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    50041 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    50101 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    50161 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    50221 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    50281 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    50341 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    50401 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    50461 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    50521 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    50581 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    50641 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    50701 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    50761 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    50821 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    50881 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    50941 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    51001 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    51061 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    51121 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    51181 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    51241 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    51301 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    51361 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    51421 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    51481 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    51541 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    51601 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    51661 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    51721 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    51781 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    51841 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    51901 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    51961 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    52021 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    52081 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    52141 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    52201 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    52261 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    52321 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    52381 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt
    52441 gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa
    52501 tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga
    52561 tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt
    52621 ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa
    52681 acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa
    52741 tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga
    52801 cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac
    52861 gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca
    52921 aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca
    52981 aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat
    53041 cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa
    53101 cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg
    53161 aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg
    53221 gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa
    53281 aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg
    53341 agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca
    53401 cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta
    53461 aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat
    53521 ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt
    53581 tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa
    53641 aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca
    53701 gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc
    53761 cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc
    53821 tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca caccccacat
    53881 ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat
    53941 aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat
    54001 tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc
    54061 taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac
    54121 tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt
    54181 cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct
    54241 gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa
    54301 acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc
    54361 taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc
    54421 tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag
    54481 tataagtttt catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat
    54541 catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg
    54601 ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat
    54661 cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt
    54721 gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga
    54781 ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc
    54841 ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca
    54901 aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg
    54961 gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt
    55021 agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt
    55081 ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag
    55141 agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat
    55201 ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag
    55261 gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct
    55321 ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg
    55381 gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat
    55441 ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct
    55501 atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga
    55561 cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt
    55621 tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca
    55681 ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc
    55741 agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag
    55801 aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag
    55861 gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag
    55921 ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga
    55981 cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat
    56041 atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga
    56101 atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg
    56161 aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat
    56221 ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt
    56281 ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac
    56341 tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc
    56401 atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag
    56461 atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc
    56521 aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac
    56581 aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg
    56641 atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta
    56701 ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt
    56761 ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg
    56821 cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc
    56881 tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg
    56941 cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag
    57001 atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa
    57061 cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg
    57121 aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag
    57181 gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc
    57241 aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc
    57301 agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg
    57361 atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat
    57421 tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa
    57481 tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt
    57541 cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt
    57601 tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct
    57661 gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt
    57721 tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac
    57781 ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca
    57841 gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa
    57901 ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg
    57961 acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata
    58021 caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata taaatattta
    58081 ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc
    58141 tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata
    58201 ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag
    58261 attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta
    58321 ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc
    58381 ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt
    58441 gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag
    58501 ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac
    58561 tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt
    58621 atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca
    58681 aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat
    58741 cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat
    58801 gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac
    58861 cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt
    58921 actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac
    58981 ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat
    59041 caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat
    59101 gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat
    59161 caggtttatt ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt
    59221 aaacgagaat cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa
    59281 taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca
    59341 agaaatagaa ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga
    59401 attaggaatc gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa
    59461 tggagaaaca gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt
    59521 aacagaatct atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa
    59581 actagtctgg tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt
    59641 ggggatacga tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa
    59701 gccttttatc ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc
    59761 tgctatatta taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct
    59821 ttccttttgg aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat
    59881 cttcacgata aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt
    59941 tatttacaga caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg
    60001 ctaatccttg tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat
    60061 ggccagcgcc gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat
    60121 actcatgttc atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat
    60181 cgattcctaa agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat
    60241 agccataagc attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat
    60301 aagctttctt taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa
    60361 gtaaccttat tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt
    60421 ttatttattg tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata
    60481 aaaaaattat ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg
    60541 aattacttca gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat
    60601 ccaaatgaaa gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat
    60661 aaaatagtta ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa
    60721 cagatttttt caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag
    60781 atggcataag ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa
    60841 cacatgtaat cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta
    60901 gcttcaggcg ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt
    60961 gtttttttat aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta
    61021 aaagaatata ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt
    61081 gtgtgaaggt gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt
    61141 ctgatacaag aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa
    61201 aagaggtcat gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa
    61261 cgttcaaata atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc
    61321 caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag
    61381 tagtcaccat ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt
    61441 gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt
    61501 gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct
    61561 gcttgtgttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca
    61621 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
    61681 tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DAYYHS010000006        60621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000006 DAYYHS010000000
VERSION     DAYYHS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.006"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(313..2205)
                     /locus_tag="ACHA6A_000335"
     CDS             complement(313..2205)
                     /locus_tag="ACHA6A_000335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968574.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="ACHA6A_000336"
     CDS             complement(2202..3929)
                     /locus_tag="ACHA6A_000336"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968575.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="ACHA6A_000337"
     CDS             complement(3945..4595)
                     /locus_tag="ACHA6A_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8968576.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="ACHA6A_000338"
     CDS             4799..5095
                     /locus_tag="ACHA6A_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8968577.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="ACHA6A_000339"
     CDS             5287..5907
                     /locus_tag="ACHA6A_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8968578.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="ACHA6A_000340"
     CDS             complement(5999..7165)
                     /locus_tag="ACHA6A_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8968579.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="ACHA6A_000341"
     CDS             complement(7177..7530)
                     /locus_tag="ACHA6A_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8968580.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="ACHA6A_000342"
     CDS             7658..8680
                     /locus_tag="ACHA6A_000342"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8968581.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8699..8860
                     /locus_tag="ACHA6A_000343"
     CDS             8699..8860
                     /locus_tag="ACHA6A_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968582.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9119..9553)
                     /locus_tag="ACHA6A_000344"
     CDS             complement(9119..9553)
                     /locus_tag="ACHA6A_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968583.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="ACHA6A_000345"
     CDS             complement(9663..11522)
                     /locus_tag="ACHA6A_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8968584.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11523..11663
                     /locus_tag="ACHA6A_000346"
     CDS             11523..11663
                     /locus_tag="ACHA6A_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8968585.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11640..12804)
                     /locus_tag="ACHA6A_000347"
                     /pseudo
     CDS             complement(11640..12804)
                     /locus_tag="ACHA6A_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13111..14535)
                     /gene="araA"
                     /locus_tag="ACHA6A_000348"
     CDS             complement(13111..14535)
                     /gene="araA"
                     /locus_tag="ACHA6A_000348"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8968586.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14551..15249)
                     /locus_tag="ACHA6A_000349"
     CDS             complement(14551..15249)
                     /locus_tag="ACHA6A_000349"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8968587.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15206..16867)
                     /locus_tag="ACHA6A_000350"
     CDS             complement(15206..16867)
                     /locus_tag="ACHA6A_000350"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8968588.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17085..18485
                     /locus_tag="ACHA6A_000351"
     CDS             17085..18485
                     /locus_tag="ACHA6A_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8968589.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18737..19816)
                     /locus_tag="ACHA6A_000352"
     CDS             complement(18737..19816)
                     /locus_tag="ACHA6A_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8968590.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19902..20861)
                     /locus_tag="ACHA6A_000353"
     CDS             complement(19902..20861)
                     /locus_tag="ACHA6A_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8968591.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20873..22384)
                     /locus_tag="ACHA6A_000354"
     CDS             complement(20873..22384)
                     /locus_tag="ACHA6A_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8968592.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22406..22969)
                     /locus_tag="ACHA6A_000355"
     CDS             complement(22406..22969)
                     /locus_tag="ACHA6A_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8968593.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22975..23838)
                     /locus_tag="ACHA6A_000356"
     CDS             complement(22975..23838)
                     /locus_tag="ACHA6A_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8968594.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23852..24748)
                     /locus_tag="ACHA6A_000357"
     CDS             complement(23852..24748)
                     /locus_tag="ACHA6A_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8968595.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24923..26212)
                     /locus_tag="ACHA6A_000358"
     CDS             complement(24923..26212)
                     /locus_tag="ACHA6A_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8968596.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26184..27701)
                     /locus_tag="ACHA6A_000359"
     CDS             complement(26184..27701)
                     /locus_tag="ACHA6A_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8968597.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27899..28789
                     /locus_tag="ACHA6A_000360"
     CDS             27899..28789
                     /locus_tag="ACHA6A_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8968598.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28862..29686)
                     /gene="nadE"
                     /locus_tag="ACHA6A_000361"
     CDS             complement(28862..29686)
                     /gene="nadE"
                     /locus_tag="ACHA6A_000361"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8968599.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29698..31170)
                     /locus_tag="ACHA6A_000362"
     CDS             complement(29698..31170)
                     /locus_tag="ACHA6A_000362"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8968600.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31807..32169)
                     /locus_tag="ACHA6A_000363"
     CDS             complement(31807..32169)
                     /locus_tag="ACHA6A_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8968601.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32392..33144
                     /locus_tag="ACHA6A_000364"
     CDS             32392..33144
                     /locus_tag="ACHA6A_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8968602.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33284..34207)
                     /locus_tag="ACHA6A_000365"
     CDS             complement(33284..34207)
                     /locus_tag="ACHA6A_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8968603.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34222..34494)
                     /locus_tag="ACHA6A_000366"
     CDS             complement(34222..34494)
                     /locus_tag="ACHA6A_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968604.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34838..36025)
                     /locus_tag="ACHA6A_000367"
     CDS             complement(34838..36025)
                     /locus_tag="ACHA6A_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8968605.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(36132..36344)
                     /locus_tag="ACHA6A_000368"
     CDS             complement(36132..36344)
                     /locus_tag="ACHA6A_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HFM8968606.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(36435..36887)
                     /locus_tag="ACHA6A_000369"
     CDS             complement(36435..36887)
                     /locus_tag="ACHA6A_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8968607.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(36900..37310)
                     /locus_tag="ACHA6A_000370"
     CDS             complement(36900..37310)
                     /locus_tag="ACHA6A_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8968608.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            37585..37797
                     /locus_tag="ACHA6A_000371"
     CDS             37585..37797
                     /locus_tag="ACHA6A_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8968609.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            37800..38012
                     /locus_tag="ACHA6A_000372"
     CDS             37800..38012
                     /locus_tag="ACHA6A_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968610.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            38152..38307
                     /locus_tag="ACHA6A_000373"
     CDS             38152..38307
                     /locus_tag="ACHA6A_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968611.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(38304..38528)
                     /locus_tag="ACHA6A_000374"
     CDS             complement(38304..38528)
                     /locus_tag="ACHA6A_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8968612.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            38606..38794
                     /locus_tag="ACHA6A_000375"
     CDS             38606..38794
                     /locus_tag="ACHA6A_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968613.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            38933..39061
                     /locus_tag="ACHA6A_000376"
     CDS             38933..39061
                     /locus_tag="ACHA6A_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968614.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            39120..39455
                     /locus_tag="ACHA6A_000377"
     CDS             39120..39455
                     /locus_tag="ACHA6A_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968615.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            39688..40629
                     /locus_tag="ACHA6A_000378"
     CDS             39688..40629
                     /locus_tag="ACHA6A_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8968616.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            40631..41521
                     /locus_tag="ACHA6A_000379"
     CDS             40631..41521
                     /locus_tag="ACHA6A_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8968617.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            41564..42364
                     /locus_tag="ACHA6A_000380"
     CDS             41564..42364
                     /locus_tag="ACHA6A_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8968618.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            42379..43230
                     /locus_tag="ACHA6A_000381"
     CDS             42379..43230
                     /locus_tag="ACHA6A_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8968619.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            43296..43430
                     /locus_tag="ACHA6A_000382"
     CDS             43296..43430
                     /locus_tag="ACHA6A_000382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968620.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            43431..43892
                     /locus_tag="ACHA6A_000383"
     CDS             43431..43892
                     /locus_tag="ACHA6A_000383"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8968621.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            43918..44370
                     /locus_tag="ACHA6A_000384"
     CDS             43918..44370
                     /locus_tag="ACHA6A_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968622.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            44476..44670
                     /locus_tag="ACHA6A_000385"
     CDS             44476..44670
                     /locus_tag="ACHA6A_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968623.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            44701..45087
                     /locus_tag="ACHA6A_000386"
     CDS             44701..45087
                     /locus_tag="ACHA6A_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968624.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            45084..45287
                     /locus_tag="ACHA6A_000387"
     CDS             45084..45287
                     /locus_tag="ACHA6A_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8968625.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            45284..45580
                     /locus_tag="ACHA6A_000388"
     CDS             45284..45580
                     /locus_tag="ACHA6A_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968626.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            45656..46072
                     /locus_tag="ACHA6A_000389"
     CDS             45656..46072
                     /locus_tag="ACHA6A_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8968627.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            46333..46470
                     /locus_tag="ACHA6A_000390"
     CDS             46333..46470
                     /locus_tag="ACHA6A_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968628.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            46880..47110
                     /locus_tag="ACHA6A_000391"
     CDS             46880..47110
                     /locus_tag="ACHA6A_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968629.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            47269..48102
                     /gene="terS"
                     /locus_tag="ACHA6A_000392"
     CDS             47269..48102
                     /gene="terS"
                     /locus_tag="ACHA6A_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8968630.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            48095..49510
                     /locus_tag="ACHA6A_000393"
     CDS             48095..49510
                     /locus_tag="ACHA6A_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM8968631.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            49522..51051
                     /locus_tag="ACHA6A_000394"
     CDS             49522..51051
                     /locus_tag="ACHA6A_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8968632.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            50969..52021
                     /locus_tag="ACHA6A_000395"
     CDS             50969..52021
                     /locus_tag="ACHA6A_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8968633.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            52023..52340
                     /locus_tag="ACHA6A_000396"
     CDS             52023..52340
                     /locus_tag="ACHA6A_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968634.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            52467..53090
                     /locus_tag="ACHA6A_000397"
     CDS             52467..53090
                     /locus_tag="ACHA6A_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8968635.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            53105..53995
                     /locus_tag="ACHA6A_000398"
     CDS             53105..53995
                     /locus_tag="ACHA6A_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8968636.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            54018..54233
                     /locus_tag="ACHA6A_000399"
     CDS             54018..54233
                     /locus_tag="ACHA6A_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968637.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            54245..54586
                     /locus_tag="ACHA6A_000400"
     CDS             54245..54586
                     /locus_tag="ACHA6A_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968638.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            54586..54957
                     /locus_tag="ACHA6A_000401"
     CDS             54586..54957
                     /locus_tag="ACHA6A_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968639.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            54950..55345
                     /locus_tag="ACHA6A_000402"
     CDS             54950..55345
                     /locus_tag="ACHA6A_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8968640.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            55347..55724
                     /locus_tag="ACHA6A_000403"
     CDS             55347..55724
                     /locus_tag="ACHA6A_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8968641.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            55725..56333
                     /locus_tag="ACHA6A_000404"
     CDS             55725..56333
                     /locus_tag="ACHA6A_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8968642.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            56333..56674
                     /locus_tag="ACHA6A_000405"
     CDS             56333..56674
                     /locus_tag="ACHA6A_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968643.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            56916..59705
                     /locus_tag="ACHA6A_000406"
                     /pseudo
     CDS             56916..59705
                     /locus_tag="ACHA6A_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            59695..60435
                     /locus_tag="ACHA6A_000407"
     CDS             59695..60435
                     /locus_tag="ACHA6A_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8968644.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            60432..>60621
                     /locus_tag="ACHA6A_000408"
     CDS             60432..>60621
                     /locus_tag="ACHA6A_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968645.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKP"
BASE COUNT        19547 a        11039 c        11229 g        18806 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa ctcttcttct
    12061 ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgctt gcataacagc
    12121 atatttatca acaatttcac ttcttcctac attgataagt acagcatctt ttttcatttt
    12181 ttctatattc gcctgatcga tcaatccttt tgtatcttct gtcaacggca gcatgactac
    12241 gatgtaatcc gaggtagaaa gaactgtttc taaatctgct tgccgaacat aatccaactg
    12301 ggcatcgcgg atagaataac ccaagacatc catccctaag ctatagcaac ttagcgctac
    12361 ttttgtgcct atcgctccaa gaccaagaag accgatggtt ttgccttgca attctcgtcc
    12421 cacataagca cttcttttgt tttctgcctg ttctaaaata ttaggacctg ttagtgtttg
    12481 taccatacga cttgcttcga ttatcggacg ggaagataag agcaaacagc acaaaacaag
    12541 ctcttttact gcattagcat ttacacctgg tgtattcatg acgatcgtcc cgttttcaga
    12601 agctttttca acgttgatcg tgttcacccc aacaccagct cttgaaattg ctagcaaatc
    12661 tggtgtaagc cattcatctt tcactttcgt actacgaata actattcctt cggctggcaa
    12721 atcatctatt attttgaaat attccccttg taacttttct atctcttgtt ctggaatagc
    12781 atcaatcaaa tgaacgtgat acataaaacc ccctccttac taacaagtat ctcgaaattt
    12841 ctgcaattag acaactgttt accatttgac ttcctagaag caaaaagagc agttagagca
    12901 gagtaggctg agtgataaaa ataaggctat gacaagttac tgccataagc cttattttcg
    12961 agagcaaaat attgagggtg agatatcttg ataaatggtg ttccgttagc tgacattgat
    13021 aataagacta aatttcaaaa aagttgaaaa gcaaatttcg tcaatctctt cttatttctt
    13081 aaaactaagt ggctttttca caacctcttt ttatctaata accactgttt ccaactcaac
    13141 taattttgcc cacgtttcaa tttgatctgt tgttagattt agcgagacaa cggtatgatg
    13201 accgccacct gcctgaatcc atgaatggac accttcatgg aaattcggtt tagttttcca
    13261 taacacacgt gcaacaggta aattaggtgc ttcagtcgtt ggttcaaagg cttctacttc
    13321 attgatcagc agtttataat gcgtaccgaa atctgccatc gatacgacta ccccttctcc
    13381 tgctttccca tcaaatacaa gacgcgccgg atcttctcta tctcccattg ataaaggaga
    13441 aacaacgatt cttggtttcg tcgcagcaag cgtcggatct acttccatca tatgggattc
    13501 caagattgct tcttggccac tagctagctc gtaagtataa tcttccatga atcctgtatc
    13561 tttccctcga gccatgattt tcagcaaccg atcaatcgct gcagttttcc agtcgccttc
    13621 cccagcaaaa ccgtatcctt ctgccatcaa acgttgtaca gctagtccag gtagttgttt
    13681 cattccatac aggtcttcaa aattagtcgt aaaagcgtta tagcctcctt cttctaagaa
    13741 acggcgaata ccgatttcct gctgagcttg tacttttaca tgttcctccc atgtcttttc
    13801 ttcataatca ccatagtcga aggtatacaa ttccttgtac tcttcaaata attgatcgac
    13861 ttcttcgtct gatacgcgat cgattacttg tacaagatct ccgataccaa aataatcaac
    13921 agtccaacca aattgaatct gtgcttccac tttgtctcct tctgtgacac ccacgtttct
    13981 catgttgtca ccaaaacgag ccacttttat tccaaaactt tcattatagg ctactgcaac
    14041 atccatccaa tcagcaatct ctttttggat ctcaggtcgt ttccagtagc caacgacgat
    14101 tttattttgt ttatttaatc gagcattgat gaacccatat tcgcggtcac catgagcgga
    14161 ttgatttaaa ttcatgaaat ccatatcgat cgttttccaa ggaatgcttt cgttatattg
    14221 cgtagctaaa tgaagcaatg gtttttgtag aagctttgtc ccgcggatcc acatttttgc
    14281 tggtgagaaa gtatgcatcc aagtgatcac acctgctact tcttctcgat aattgacctc
    14341 tttcatgatt tttgtgatcg tatcagcagt aactgctaat tcttgaaggc ggattggata
    14401 aggcaattgg ccattttcat tcagttcatc gaccatttct tgtgcatgtt tctttacttc
    14461 tcgcagtgct tcttccccat ataaatgctg tgatcctaca acaaaccaaa attctttttc
    14521 tccaatattc aacataatta gccactcctt ttatttgttt tgtccgtaat acgcattttt
    14581 tccgtgtttt cgtaaataat gtttatctaa aatccgttga ggcagctctt ctgaaaatga
    14641 attgagctgt ctggtaaata aattcatctt gcatacttca tctagtacga ctgcgttcat
    14701 taccgcactt tgcgcatctt tcccccaagt aaatggaccg tgtccgtgta aaagtacccc
    14761 tggaattgct aagggatcaa ttttgcgttc tttgaatgtt tctatgataa cttttcctgt
    14821 ttcatattcg tacccgctgt cgatttcctg ttgtgtcaaa aatcgagcac aaggtaccga
    14881 tccataaaaa gtatctgcat gcgtcgtccc catcgcagga acatccaatc ctgcttgtgc
    14941 ccagatcgtc gcccatgtcg agtgagtatg gacgattcct ccgatttgag gaaatgaacg
    15001 atataaaaca gcatgagtag gcgtatccga agagggattg agtttacctt caatgatatg
    15061 attgtccaag tccacaacga ccatgtcgtc tgctgtcatc tcttcataac tgactccgct
    15121 aggtttgata acgaaatatc gtgtttcagg atcaaaagcg ctcacatttc cccatgtata
    15181 tttgacgagt ccgtgtttag gcaagtcaag atttgcttgg tatacttctt ctttcagttg
    15241 ttccagcatt ttctatcact cctttttctt gcaattgatg aatcgctgtt ttttcaatgt
    15301 cgatcccctc acggtaacgt ctcataaata tctgatagcc tgctatttct tctgctgaag
    15361 gtgtgatggt gattccttgg tcttgtccaa acacgtcttc tgccaagaag ttttctaaag
    15421 acttcccatg tcgatccatc atataggctg ctaataacgc gattccccac gctccacctt
    15481 ctccggctgt gtccatcact gtaactggtg cctccatagc agatgctaga atccgctgtc
    15541 caacttctgg tgttttaaaa atccccccat gccccactaa acgatcaatt tggacattct
    15601 cagatttcaa tatatccatt ccgattctca ttgcaccaaa agcactggct aaatggatcc
    15661 gcatgaaatt cgctaaatca aaacggctac ctggtgtacg tacaaataat ggccttcctt
    15721 catttactcc tgtaatgttt tcaccggaaa aataaccata agaaagtaat cccccgcaat
    15781 ccgtatcgcc ttctagcgct ttgttgaata ataccgtgaa taatttatct ctgcttatct
    15841 ccatacccaa agaattggta aattcttcaa aaatactgac ccaagcattg atatccgaag
    15901 agcagttatt cgtatgaacc atgcctacta gttctcctga aggtgtagtg accatgtcga
    15961 tttccggatg gacattttct agttcttttt ctaatacgat catagcgaag gcggaagtac
    16021 cagccgaaat gttccctgtc cctttggcta cactgttcgt agcgaccata ccggttcctg
    16081 catccccttc tggcggacaa accgggattc ctggtctcaa atttcccgta gggtcgatca
    16141 gataagcccc tgattcgctt aacttgccag cttcttctcc tgctactaga actttaggca
    16201 aaatgtgatc caagtcaatc gctacgtgcg ctttttcgat taactgacca aaaatatttt
    16261 tcatttcttg atggtaggtt ttcgttgaag gatcgatagg aaacatgcct gatgcatctc
    16321 ctacgcctag tactttctca ccagtaagct gccaatggac ataacctgca agcgttgtca
    16381 aaaaattaat ttctttcaca tgttcttctt tattcaaaat agattgatac aaatgagcga
    16441 tactccatct ctgcggaata ttgtattgaa atttttccgt taatattcgt tctgcttctt
    16501 cagtgattgt atttcgccaa gtacgaaatg gtactagtaa ctgttccttg ctatcaaatg
    16561 ctagataacc gtgcatcatt gcactgaacc caatcgaccc gattgtcgta agattctcgc
    16621 catagatttc gaatacttct gctgccattt ttcggtaact gacgcgcagt cctttccaaa
    16681 tatcttctaa tgaataggtc cagataccat tttctagttt gttttcccac tcataactac
    16741 cagcggcaat aggagtaaaa gatgaatcaa tcaatacagc tttgatccta gttgatccaa
    16801 actcaatccc taaagatgta cgaccatcta tgacatctaa catactttgc gcatttactt
    16861 ccttcataat gatttaactc cctccttctc gtcaaacctg atcaaaccgt ctttttagta
    16921 gcgcttacaa tgattattgt atacttttgt acgtacatgt caagcagtta tttacaagaa
    16981 tacaataaaa aacacttgtt ttgtacgtac ataagttata aaatgaaatt gcttacaaaa
    17041 tctgatcaac cgccttctcg aaattctaga aggagagaat acttatgaaa aaagaaaaaa
    17101 aagtttcctc atcgtttatt tatttttttg gcgcatttgg cggcattcta tttggatatg
    17161 atattggtgt tatgaccggc gccttacctt ttttacagca tgattggggt ttggcaggta
    17221 aagcttcttt gataggatgg atcacgtctt cggtcatgtt aggtgcgatc cttggcggct
    17281 ctttatctgg tcttctttct gataagcttg gacgacgaaa aatgattctt ctttctgcac
    17341 ttatttttat ggctggttct gtcctttctg catctgcccc tcacaatggt tcttattttc
    17401 tgattgcagc aaggattctt ctaggtttag ctgttggcgc tgcatctgcc ctagttcctg
    17461 cttatatgtc tgaaatggct cctgctagac taaggggaag attgtcaggg atcaatcaag
    17521 tgatgattgc ttctggcatg ctcctttcct atgttgcaga ttatttgcta aaagggttgc
    17581 ctgaaacgat ggcttggcgc gtgatgttag ggctcgctgc tgtccccgcg ctgattttat
    17641 ttttcggtgt tttagctcta ccagagtcac cgcgttttct tatgcaatct ggccgcttgg
    17701 aagaggcaaa aagagtattg aactatattc ggacaccaaa agaagctgaa caggaattcg
    17761 aacaaatcca gctgaatgtc aaacaagaaa agacaaccgg aacttcttgg catactttgt
    17821 ttttagaaaa atatcgttcc ttagtcattg ccggtattgg tgtagcagta ttccagcaat
    17881 ttcaaggagc taatgctatt ttttactata ttccgctgat cgtagaaaaa gcgactgggc
    17941 atgcagcgag cgatgcattg atgtggccga tcatccaagg aatcatttta gttgctggtt
    18001 ctttgctctt tttggttatt gcagacaagt tcaatcggcg aactttattg aaaataggcg
    18061 gtagcgtgat gggactttct ttcatcctac cagctgtttt aggaactgta ctggatgctc
    18121 atacaaattc gttgttgatt ctcttgtttt tatgtattta tgtcgcattt tattcttgca
    18181 catgggctcc actgacatgg gtaatcgtag gcgaaatatt cccactagct gtccgcgggc
    18241 gagcatctgg cctagcctct tcttttaatt ggattggttc tttcctagtt ggcttgcttt
    18301 ttcctgtgat gactgcttct atgtctcaag aaatcgtttt cggcattttt gggatcatct
    18361 gctttttagg ggttttattt attcaagaga tcgtaccaga aactcgtggg aaatctttag
    18421 aggaaatcga acaaagtgca tctaaaaaaa cttatcctaa acgtatttct atccatcatt
    18481 cttaaagagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaac cgcttagttt
    18541 ccagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgatgaata ttgtgacttg
    18601 tgaccagttg gtaggagaac accgtttagt ggagaggtgg tgaaagaaac gttcagcctt
    18661 gagcgatgac aaaagctctc acaaacattg cgacgagtaa ccgtaggagc aataagaggc
    18721 ggtaaaaacc cgtctcttat ttttcgtttt ctttcactgt ttcccgttca actagtacag
    18781 gttcatagag aatatttggc tgtgcctgtc ctttttcaat cgttgtgacg atccaccgtg
    18841 cggcatcttg tcccaacttt tcttttggat gcgtcaatgt tgtcaatttt ttattcccta
    18901 aatggcttaa agaagaatcg tctgttccta cgatcgatag gtcatctgga atatgatagc
    18961 cattttctgt caacttatct agcattaaac tagctatttg atcgttatag caaatgatgc
    19021 catctaaagc ttgttcttct ttcaaccgtt gtactgtttt ttcagcgatt gtttctcgtg
    19081 tttccgtggt gaaagtaatg atatcttccg tcaaaaaacg cacattatac tgttcacacg
    19141 cgctgataaa acctttcatc ctgtattttc cttgaagatc gtcgattttt gtgatgaaca
    19201 acagacgctg atgtcctttt tttatcaaat attctgctgc cataaaaccg actttcacat
    19261 catctaaaca aatataagag gcatttaact cttcataagc agcattgatc atcaccatcg
    19321 gaatgccttg ttctctcaaa gcaacataaa gtgctaagtt tgggttatac tgattacttt
    19381 tcgtaggctc aacgatcaaa ccatctaccc cataagcaat cattttcttc aaacactctt
    19441 tctccaattc gtgatcgtta ttcgtactgg ctaaaagcaa tgaataccca gattctcgta
    19501 attcattttc gattcctcgg ataatcgacg gaaaaatata atcagagaga tatgtggtga
    19561 tgacaccgat cgtttttgtt ctgttcaatg gtgttttttc tttcttatag ctgtcatcta
    19621 catatgtgcc cgaccctttt tctttacgca gatagccctc atttaccaac acagcgattg
    19681 cttgccgaat cgtatgtctg ctgacgtcat atatttcttg cagcttgaat tctgagggaa
    19741 tggttgtatt tacttcgtat tcctcacgaa ggatcttttg ttttatctct tctgcaatat
    19801 cttgatattt tgccatttgc cttgcccctt tttatttgta cgtacatata tcataacgag
    19861 tctgcaaggt ttattcaagc aaaaatcaag gtttcttgtg ctcataaggc atacggaaca
    19921 ggttcaccaa attctggaaa tccttgctca ttccacgtaa agcattgcgc gttcgcgtgc
    19981 cgattaggat tgtctaacgg atcccctttt tggttcttct ctggtcgagc atgatagatc
    20041 aaaatatcaa agcacccgtc ttctgaaacc gtaaacgagt tgtgaccagg accaaattgc
    20101 ttattttttt tagaagaaac gaatactggc tccgataatt tatgccatga ttcttttaat
    20161 aaaagattct tgtgctcatc agcccacagc aggcccatgg catagttttc atctgttgca
    20221 ctaccagaat acgtaatgaa tattttccca ttgcgagtaa ggactgctgg accttcattt
    20281 actgaaaaac cccgtttttc ccattcatac tcaggtatag aaaggaggat ctgcttcccc
    20341 tttaacgtcc aaggattatc catttcggat agataaagat tagaattccc agtaatttca
    20401 ggatcttgtt gggcccacac ataatatagc tttccttgag aagagaaagt tgttccgtct
    20461 aaactgaaag tttccctttt cgtttttatt tgtccctttt ctatccaatc tgtattcatt
    20521 ggatctatat gctgattttc aagtacgaac atgcggtgat gatgagattc atcacgtata
    20581 gttttatgat tgcttgcagc aaagtaaata taccattttc cattgatata atggacttca
    20641 ggtgcccaga tcaattcact catcggtcct tcttcatgag cctgccatac aatcaggctt
    20701 gcagcatgtt ctaattcatt cagtgacttt gcccttctta attcaatcac ttggtagcct
    20761 ggaaccgatc ctgtaaaata ataataacca tctgtatgtt tatataccca aggatctgcc
    20821 cgttgccgaa cgataggatt attataaagt gtcttcatca taacaaattt cctcacattt
    20881 tctttattct aattacattc caagagtatt tttctatccc tacatttaat tcgtcatgtt
    20941 tttctattga aaaatagttt atttcaggtt ttattttttc tggttcttca cgtgtatttt
    21001 tgtccaacag atcgctactg gttaatgtgt agtgctctcc ttgatctagc aaattgaaat
    21061 catgttcaat cgttatcttt tgttgttcat ttgtgcgatt gatcaagaaa atagtcagct
    21121 gattgccgtt atctacgatc acactgtcca cataaggaac attttttatc ttttcacaat
    21181 cataaacagg gcaaatgatt tgttgtttca atacttgacc tttagcatat ttcgataagt
    21241 gttgcatcac ataataagtt ggattcctcc atgcttgtcc tttagcatct gtcagtatta
    21301 aaggtattgt gtttactaac aatgattgac aactaatctt tacgcgatct gcgtgtcgta
    21361 aaatagccag ccctaatgaa ccaaataata atgtatcttc catagtaaag taacaccaat
    21421 cgatgggact accgacttca aaatccctat gtcttttttc cggttgggca tgtacattcc
    21481 attcatcaaa cgctagatac attgttttct cacttctcaa atatcctttt acataatcac
    21541 aagtagcaac gatttcttca atttgacgat cgaatcgata agcttttgca aggtatgtcg
    21601 ctgtatcatc acgctcattt ttaggaggtt tagtcaagtc ttcttcatca tacttatcaa
    21661 tatagttatg caaagctaaa taatctacat tctcataagc ttcttctaat gcgatacgat
    21721 cccattcagg gtacgatggt aaacgtggcg tagagcttcc aactaagatt gtttcaattg
    21781 aatcatccac taacttcatg acttttgagg cttcatttgc taacctacca tattcataag
    21841 cactcttagc ccccacttgc catggaccgt ctagctcatt ccctaaacac cataatttaa
    21901 cgccaaaagg ctttttttgc ccatttttta ttcttaaatc actccagaag gttccttttg
    21961 gaaaattaca atattctact aaattttttg cttcatcaat tccttttgtt cctaaattca
    22021 ccgtgaatat cggtttcgct tgtacatgac tgatccattt aaaaaattcg tggagtccaa
    22081 aagaatttgg ttctatacct cgccatgcta aatcaatttt ttttggtcgt tgttctttcc
    22141 tacctacagt atccacccaa tcataaccag atgtaaagtt tcctccagga tagcgaatga
    22201 cacctaattc taattccttt acataagaga ggacatcttg tctaaacccg tcaccatcag
    22261 ctgtttcatg gcccggttca taaatccctg tatagacgac acttcccata tgctcaacaa
    22321 atgagccaaa cagccgagaa ctgatctctc ctaccgtaaa atcctctctg atttttactt
    22381 tcacttcttc gtccaacagc tttccctacc tttcatttcc tatagtcaag ataatagata
    22441 caagtttcca tacgccacca gcaaagataa aaataaaagg agggaaaaga aatatgagca
    22501 gtacaaatcc taataacaat acgcaacaaa gaatatttct caaaaaatca aaaaatactt
    22561 tccatgcact ttgttcaaaa atttgtaaaa atgtgatatc tttgttttca gaaatcacat
    22621 atatccagga taaaagaaac atggaataaa taagtaaaac tacatatcca ctaattgaaa
    22681 gtacgattgt ctgttcttct aataattgat taacaagaaa ataaataatg atcgttaaca
    22741 gactaaggaa caagttctta ccaaaatgtt gctttattcc ttgaaagaaa aaataaatga
    22801 tatttcctga tccctcacct ttccattgcg ccattacata aaaaacagca catgtagcag
    22861 gcacgatagt aataacaggc aaacacacga ttacccagag aatacctaat aagatgtaat
    22921 cagaaaccac aatcaactgt ccataaagtt tagtctcaac tattttcatc ttcctcactc
    22981 tttcattcca gacatagctg ttccttgaac aaaatatttc tgtgcaaaaa tataaaccac
    23041 tagtaatggc agcattgaaa taaccgttcc agccattaca ggtccataaa aagaaatgtt
    23101 ctgccctgta aataaagcta accctgctgg taaagttctc atttttgaac tacttgtcaa
    23161 tatcaatgga taaagcaagt cattccaact attcatcagc gtgaaaatcc ctaatgataa
    23221 aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa atattaaact cacttaatcc
    23281 atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta ataaaaaagg aacgcatcat
    23341 gaagatacca aatgcagttg tcatacgagg aatgatcaaa cctgcataag tatcaagcat
    23401 gccaagcaaa ttgacttcaa taaatagagg aatcatgaag acttggaatg ggatcatcat
    23461 tgtcagcaaa acgaaataga acaaaactga ttttcctttg aaacgcatac gagcaaatgc
    23521 ataccccgca agagaatcaa agaatacaga agtgatcact gtccctccag caaaaatcac
    23581 tgtatttttg atgtaatcta ataaagggat cgttgtccaa accttaatat agttatctag
    23641 ggtatagcta tctgcaaata ttctaggcgg aaatgagata atgtcttttt cgtgtttaaa
    23701 agaagaaata atcagccaca aaaaaggaaa cgcaataacc aacaatataa ctagcatact
    23761 aatcgttcga atgatttttt taccactaac tgcttttcga ctattcacag taactgtaat
    23821 cgagctttct ggtgccatac aggtttccct cctatttcat ttgtttttct ttccgtgaca
    23881 tgtacacatt agtggagata gctatagttg caacaatgat aaacaaaacc gaagacagtg
    23941 ctgatgcata gcccaattca taaggtgctg taaatcctcg actgtaaata tattgaacag
    24001 ctgtttctgt cttaaattgt gggccaccag cggttgcaac atatacttgg tcaaacactt
    24061 gcatcgaacc aatcagattc gttaaaatac aaaaacctaa ggagggaacg atctgaggta
    24121 gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc catttgagca gattcataca
    24181 aagagggaga gattccctga atagcagtca agagaatcgt catagtaaca ccaaaattct
    24241 tccaaacagt catgatggct accgtcggca tagcaagggt tggatcgcga aaaaattgag
    24301 ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc ccctacattc ggatcaagca
    24361 acatagaaaa aataatcgca attgacgtca atgaacaaat aactggaatg taaaatgtcg
    24421 aacgacaaaa acgattgaat ctggtatttt tagataatgc tactgctgct aataaaccaa
    24481 cgataatttg tgttggcgtt tcgagtacgg tgaaatataa agaatgaatc aacgaattaa
    24541 ctgctcgttc atcactaaat atacgtgtga aattttcaaa acttacaaaa tttcttgatg
    24601 taaagaaaat attcaaatct gtaaaactaa tagcaaatgt tcctaaaagc ggtatgacag
    24661 taaataataa taaaactaaa aaagatggag ttaaaaacaa atacgcagca cgatcaggac
    24721 gattccaata tttggtgaat ccgttcatat agtccacctc atctttcatt ttgataaaat
    24781 atacaatctt ggtttaaacg aatctgaata agatatccga acaatcgctg tgatttttgc
    24841 aataacacct atctgcaaat actaggtcga tcattcagat atctgtatct tgccaacagt
    24901 ctatcctgta taaagttctg tactattttt gcagtaattg attgatggca tcatctgcat
    24961 cgttcatgag tgtctcagga tcttctcctg ctaataattt ttcaatcagt ggattgatga
    25021 tatcgttatt gatcgcagag atttgggtaa aacctggtaa atatggctct gcatagtcca
    25081 tttgtttcga taattcagat acaataggat cattttttac ttcttcatct tttgctactg
    25141 aatccaagta ggcaggaaaa ccattttcga tactccattt tttaccaatt tcagttgtat
    25201 tccaatattt caaaaactca tagacagctt cttttttatt attatctgta cttgccggaa
    25261 tggcatatcc tacgccatct aaaatagctg tttttttgtc tgaatcactc ccttgtggta
    25321 aggtagtcac attataatca atctcgtttg ctttcaatcc gttatttaac catggcccat
    25381 tgatttcaat ggctagttga ccagcattca ttaaattgtc catttcggcg ccagaaatcg
    25441 attctggacc agtcttgtct acatggatca ttttttggat cgcttttaac gttttaaggt
    25501 tctcaggaga cgcaaattca gacttagtat aatcatcgtt cactaattta ccgccatttg
    25561 ctaacatcca gttatataac ggtgcagttc catccttatt gaagacaaaa cctgatacat
    25621 ttttactact atcagttaat tttggtgcca tttctgtcaa ttcttcccaa gtagtaggtg
    25681 gttgttcagt atctaatcca gcttgtttaa acaaatcatt attccaatac atatacatgc
    25741 cttgtacctg catggggaca aaatactgtt taccttctac ttcacctaat tcaatagccg
    25801 tttctttaaa atcagattta tctactccat catattccca gaaatcgtct aatggttgta
    25861 ccgctccgtt ttctacatac tgtgcaaaat ctggataatt taatgcaata aagtcaggtg
    25921 cttttttaga agaaatcgct gtaggaagtt tttcattcaa attggcccaa gtcatgatat
    25981 ccatttcaat tttgatgttt ttatcatttg ttttattaaa ttcattaacg atttcttgca
    26041 atatttcacc atcagatgca gtaaacccat tccaaaaagt caaagtcact tctttatcag
    26101 aatcagattc tgctgaactt ccgccaccgc aagctcctaa aatacctgtt gcagctacca
    26161 acaaagagcc aattaaccat tttttacctt tcattttttg ttcctccttc atctgataaa
    26221 cgacatctga ccacattcca agatagcggt ttcaataaac aattgatttt cccatcttca
    26281 gcttttacat ttgttgaatg acttggtttt acttgttcac ttttaggaag attagttgct
    26341 tttgtatcaa atccactcat ttcagaaaaa tcaatcactc cctctaatga taattcagac
    26401 aagtgaacag ttagattcat ttcttcttct ccacggttaa ctgcaaatat gacaagttct
    26461 ttgtcattta ttacggctat actttctaaa tacggcactt cttcaaaatc ttccgtcgca
    26521 tagtttgctg aacgaatttg tgggcttaac acctctcctt gtccatacac agcagcttgc
    26581 ataaatggat aatagatcgt ctgtctccaa gcttctccgt ttgtttctgt cctgattggt
    26641 gcaataacat ttaccaattg agcaagacaa gcaatcttta ctctatctgc atgtttcaat
    26701 aaagtgatta atagacagcc taatagtaat gcatcttcaa aattatagtt atcttctagt
    26761 aaaggaggag caatctgcca aggcgctgct tgctcatctt gttccgctga atgataccaa
    26821 atattccatt catcaaagga caggttgatc tgcttcttac tatgtttctt cgccttgact
    26881 gtatcgcaca tcgctgtcac ttcagaaatg aaccgatcca tgtctaagga acgtgcaaga
    26941 taatgagaaa gatcattttt ttggttgcca taatattgat gcagagacaa ataatctatt
    27001 tgatcgtacg cttgatcaag aactgttaac tcccaatcac caaatgtagg catttgccga
    27061 ttcgaacttc cgcataaaac cacctcaatt gaatcatcta cgagtttcat cgccttagcc
    27121 gtttcctctg ctaaccgtcc atattcttcc gctgttttat gtccaatctg ccaaggaccg
    27181 tccatttcat tccctaaaca ccatgtctta attccaaaag ggctctcttg accattcgaa
    27241 cgccttaaat cactccaata agttccttta ggaaaattac aatattcgac taaattacga
    27301 gctgcatcta ctccacgagt acccagattg acagccatat tgatttcggc ccccacatgc
    27361 tgacaccagt tataaaattc atgaatccca acttcattcg tttcaattgt ccgccaagct
    27421 aaatccaacc ttctaggtct atttgctatg ggcccaatac tgtcttccca acgaaatcct
    27481 gacacaaaat ttccaccagg atatctaatc atgggaatgt tcaattcttt taccaagtga
    27541 atgacgtctt ggcgaaatcc acttttatta gctgatggat gatctggctg ataaatccct
    27601 tcatatactg ctcgtcccat atgttcaata aatgaactgt acaaccgcgg atcaactttg
    27661 cctacacgct tgacactact aatcaacatt gtggcttcca taactgacct cctttaattt
    27721 atacatgaat aatacacctc gttgaaagcg ttgtcaataa tataaatgtt aaacttactt
    27781 attttttatc gattttaatt aaccaaaata ttaataatga ttaaaacaaa taatttaata
    27841 taacttttat taacaaatat gttaatatat aagaaaaacc ttttaagagg tgatatatat
    27901 gcaactacaa ataaacgaag aatccttacc ggtatatgaa gcattagcta gtaaaacaag
    27961 gatcaagatc atccagctac tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga
    28021 acttggagtg agcagtgcta tcacgacgat gcacgtgaaa aagctagagg aagcaaacat
    28081 catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga
    28141 tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat cgatacctat
    28201 tggtcactat acaaattatg caattgaacc aacatgcggt ttagctacga tccatgattt
    28261 tatcgggaaa gtggatgaac cacgctattt tatggatcct cgaaggatgg atgcccgcat
    28321 tctctggttt accagcggat ttgtcgagta tcaggcacct aattttttaa atacagagga
    28381 tacattggag atgttggaag tttctgtgga aatttcttct gaatttcctt tttcaaatga
    28441 caattggcct tcagatatca ctttcagcct aaatggtgtg gagctaggaa catggacgag
    28501 ccctggagat tttgctgata tccgcggtaa atatacgcct gattggtatc cagataatct
    28561 taatcaatac ggcttgctga agacgatccg aattaccaaa cacttgacga atatgaatgg
    28621 agaacccttg tcaaacatca cgatcaacga catccctaaa gagcaagaca cttggcattt
    28681 gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc tgtactttat ttggtaaagg
    28741 attcggtaat tacgatcagg atatcaaatt aaaagtttac tacagctgat atttttcttt
    28801 tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt ttgctttatt
    28861 gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt
    28921 tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct tctagataat
    28981 catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt ggtttcccat
    29041 cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc aatgcttgaa
    29101 gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg gctccaccat
    29161 caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc cctaatacgg
    29221 ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt gctttgatat
    29281 ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt acttgtgcat
    29341 caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag tcaagagcag
    29401 cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg ataaattgat
    29461 agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc aaacggcctg
    29521 ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga aacggatatt
    29581 ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt ggatcaattg
    29641 ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat tctcctccta
    29701 tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag ccattttatg
    29761 gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt ctcgtttata
    29821 ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt ttagtgcagg
    29881 taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc gagctgtaaa
    29941 gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga tagcttcttc
    30001 ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc catcaaaatt
    30061 tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg cattgcttga
    30121 taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta gcttgaatac
    30181 tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac cccatacatc
    30241 aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt cattcgaagc
    30301 atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac gcttagatat
    30361 ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt cacctaattc
    30421 tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag tgtcgactaa
    30481 gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat catttccata
    30541 agattggact aacgaatgag catgggtccc gctagctggt atcccaaata tcttcccagc
    30601 acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc cccagactgc
    30661 tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt ctccgatgac
    30721 agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca tattcaacag
    30781 tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatta atggttcatt
    30841 tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt caaaattttg
    30901 taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag caatatcgga
    30961 atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg caaaaaccgc
    31021 gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat gtaaatcagc
    31081 tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg tatgcagcgt
    31141 caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc aagttttcat
    31201 ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt ttttctgaaa
    31261 aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta aaactatcct
    31321 ttcttattgg tatttaacct aaacgagaaa gaatacttat tttattcaat gtatccgaga
    31381 cacgaagaaa ttagcgtttt ctatttttct tacttgataa aataagtaga ttttcctact
    31441 acaatcgtag ccgttaagat accgatgatt gaacaaatat aaccaaatgt ctctacaaca
    31501 taatcaatgg caaaagtttt tgaagcaata atacgtcttt ccttttttgc ttcttatccc
    31561 caaagaaacg gatataactt attatgtatt gaattttttt ggaaaggcga attatgacaa
    31621 gatatacctt gcgttgaaaa aactcgcgga aaaaatatca cacgttttta aaaagataca
    31681 aagtaagaaa gcagctgtca atagaatgga aacaaaaatg gcgagacatc aatcagtctc
    31741 gccttgtttt ttattgtata cattagatat ggagcaggtt accataaaga tgattattag
    31801 tcgttattaa atagaatcct tcgtattttt ttgttctatc actacaatat ctttatttgg
    31861 acgtttgaat tgtattccgg cacgttctag ctcctgtttc actcctacat ggactttgac
    31921 atcttctcgt gtcattttta aaacgtgatg aatcgcatag tcgatttctt gccttgtgat
    31981 ttttttcttc gtattgatca tcacgacttg ctttttatca tatgtgtcta ccaatagcag
    32041 ttgtgttcga tctaacgaaa ataatttgta ataattaata gccgtattcc caaaaaagaa
    32101 ttccactact ttaggataaa atgtcttaat atttaaatta ctattcacag gtgtttcgat
    32161 tagcttcatc tcattcctct tttctatata gtcgatttag atccaatctt aagttatgtt
    32221 accatctttc ctagcattaa ggaatcggtt acaattgaac ctttaaagac aaaaggattt
    32281 accttttatc acttaatagt ataacataat ttcgtgagaa aaaaatacaa ttttttcttg
    32341 accatctcgt tacgtaaagg attatgctta catttgtcag gaggaaaaat aatggagtac
    32401 acaataaaga aaatgtcaga gatatcagga gtcagtccga gaaccttgcg cttttatgat
    32461 gagattggac tgctaaaacc agctaggatc aattcttcag gttatcggat ttatggcaaa
    32521 aaagaagtcg atcgtctgca acacatctta ttttatcgaa caatggcatt caagttggat
    32581 gatatccaag aggtcttaga caatccatct tttgaccacc agaaagcttt aatcaagcat
    32641 cagcaaatgt tgctggaaaa acgggcacaa atcgataccc tcttgacaac tgtccaacag
    32701 acacttgata tgtacgaagg aggaagaaaa atgagtgaca aagaaaaatt tgaagggttt
    32761 aaacaacaaa agttgaaaga aaatgaagaa tcctatggac aagagattcg acaaaaatac
    32821 ggaaaaaaaa aggttacagc atccaatgaa aaatggatga atatggataa agaaacgtat
    32881 gaagaaatgc aaactgttga aaagcagatg ttatttgatc tcactcttta cttaaaagaa
    32941 ccagaagatg aaagcttagc agaccgtatc ttccaagctc ataagaagtg gttctcttac
    33001 agctggcctg aatatcagcc aaaagcgcat aaaggactag gaatgatgta cgaagccgat
    33061 gaacggttca ctgcatatta tgacgagcga agtggtcatg gtgcagcaaa agctttaaat
    33121 gaaattatcc aaaaaaaggc gtaatctttt acatgatcca acaaaaaagt acacgtaaaa
    33181 tgacctaata acaaaacagc agacacccaa gttagatttt tatgctaact tttggtgtct
    33241 gctccattct ttcacaagtt ttttcatcct actttaaata tattcatcgt tgaataaacg
    33301 tataaggtat tttcacttta tccttttttt ctgtaagaaa caattgaaat gccatttctc
    33361 cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag aagctcgctg atcagcaaat
    33421 tatcgcgtcc aatcaccact ggtgtcttat cttgcagata gctttgcaag atgcctgctg
    33481 caaccatatc cgaatttgta aagacaccat caactccaag ctctttaaaa aagaagcctg
    33541 cttgttttcc tttctctgca tctatacaat cccaaaaaac aagtgattca tcaaaagatg
    33601 gaaaaagttg atgacatagc tgcaaagtga tttttgagtt ataacttagc ctgccacttc
    33661 gccccaacgt gatgcccaat tgctttatac cttgctgctt caaataagta agaccttctg
    33721 taattgattg ttctcgatca ataaagacac agcttgcctg ttttcccttg atttcttcac
    33781 aaaaaactag aggtccatat ttttgatatg ccaataaatc atctagtgta ttggcacgag
    33841 acgtaatgat caatccatcg aaagctttag ctgcaaattc ttcaagatac cttcgctctt
    33901 tttctttatg atagcccgtc ggtaaaagtg tcactttgta atcataatca aacgctgctt
    33961 caatgatccc actgatcaac tgatcaaaat acggatgatt ggtataaggc agaatcactc
    34021 caatgctttt cgtttttcca taacttaact gtctggcatt tgcattaggg atataatcga
    34081 cttctttcat gaccgcaagt atttctttcc ttttttcctc atctacatat ggatgtccat
    34141 tgattacacg tgaaatagta gaaactgaat aacctgtgag ctttgcgata tctcgaatgt
    34201 ttgccactgc tgcttcgcca cctatacaaa aagattttct tgtaaatagt ctaccacagc
    34261 tggaccgcat tcaaaaaact gatacatttt tccttcatgc gaaaaactga tccgctgccc
    34321 gccccaatat atatcttttg tcaaattttc gatttcattc attggtattt cttttactat
    34381 attcctattc aacccttgaa aactgtacaa cgttaatacg gatcccttga actcgacaaa
    34441 cgcataaaaa taattgttcg ttgtgccatc tgttatctct acataagtat tcattgcaag
    34501 ccctcctttt gatactttga gtatagtgta tgaaacgcgt ctcatagcaa ggagtttgtt
    34561 ttgccaaaga aaagttttat tcctctttca ttattaccaa agtctcttat tttaaattga
    34621 atacattata atgatactgt aaatatatct ttattattag tgaatggagt caagtccaca
    34681 atcttgtgta aaagttcaac ctcatcaaaa gcacaatgtt ttgcgttgtg aacctaactg
    34741 ccatgccttt ctttctgagg agagaggtca ggagcagtag cgcagcgact cttgatgtct
    34801 ctcctcagaa agaagagact cgcacaggtt catggtgcta aatgttattc gaattaaatt
    34861 ctaaatattt tcgtggagaa gccagccact cagtgtcttt ttccatcaaa agtgttccaa
    34921 ttatccgagt gatggaattg atatttggaa aaatccgaat cactcgttct ctccggcgaa
    34981 tctcttcgtt caaccgttcc aacacattgg ttgtacgaat ccttcggcgg atattttcag
    35041 gaaaagacaa aatttgaata gcatcttcaa agccgttttc taaaatgtcg caagcagcac
    35101 ttaattttga atcacaacca tatttttcca agaagtgctc ttttctttca cgtgtcaatt
    35161 caagttcaga ggctttaaag atactcttca actcgtcttt tacatcagaa gaaactttct
    35221 ttggcagctt atcaaaaata tttctcaaaa agtgggcttg gcatctttgc cagctcgcac
    35281 ctaaaaagtt cttgcgaaca gccttaacta accctttgtg tgcatcagaa atgaccagtt
    35341 ttacaccgaa caaacctcgt tgaacgaacg attgaaaaac ggtatcccaa ttatcttccg
    35401 attcgccgtc ttgaacatca aaaccaagaa tcgtacgtcg tccatcagaa tcaatgccga
    35461 taccaatata tacacctttt gagacaatac gatgattttc ccttactttc atatacaggg
    35521 catcacacat gaaaaatgga tattccgagc cttctaggct ccggtttctc cactcaaaaa
    35581 caaatggatc caactgtttc atcagatttg aaacgaatga tttagaaaca gtcgcaccat
    35641 ccgttagcag ttcaactgtt ttagttactt tgcgagtgga gacaccagaa atgaccattt
    35701 caatcatggc agcgatcaaa gctttttcgc ttcgctgata tctttcaaag atagtaggac
    35761 taaacttacc gtctctggtt cgtggaacat gaagttctaa cgtaccaatt cgagttgtaa
    35821 acgaacgatc gtagtaacca tttctttgtg attgccgttc ttctgaacgt tcataggcat
    35881 ttgcctgaat atattgattt cgttgttctt ccatcagctg attaaacatg atagtcataa
    35941 gtgtttttgc ggtatcattc gcaccgcttt cgtttataat tgcttgaatt tcttcgcttt
    36001 caaaagtaaa atgtacttga gtcattgata tcagtcctat tcgagtatgt tttctgcaaa
    36061 aaacattgta ctcctaactg agatcaatgg cttttttgat gcttcacttt tacacaatta
    36121 tatggacgta atcaattaat atcatttttt aacatagtaa tttgtttatt taaatctgat
    36181 agtgtttctt ccttacttaa tataaatctt tctctatcat gtaatttatt tagtttttca
    36241 tcattttttt gttctaattc ataagcttct ttttttattg tgttagcttt cgacagttta
    36301 gtgattccta taattaataa aataagtcca atcaataata acattcaaaa ctcctcattt
    36361 ctttgatata attattgtag agtttactca gaaatgagga aagagtccat gtgaaagcat
    36421 gggctttttt atttttaatg ttgtaatact aaatgttcaa gattatgagg aagccctaaa
    36481 tattcaataa tttggaactg actcatatca gataaatctt gttcatctat tgttctagtt
    36541 aacatttcaa ttgcaaacat attagcttca gcttctatac cataaatcat acaactaagg
    36601 ttgtttgctc taaaagctgc tgtgcaaata cctttatgga gtctaacgtg ggctatttca
    36661 tgccataata caaatctttt catagaaggt gataggtttt gattaattaa tatcacataa
    36721 catcttttat tgtgtatcgt ttgtcctaat gttttattcc ctaaatcagt ttcaattaaa
    36781 tctatatcag aatatttgat tattttatat ggattaatcg catcagatcc aaactctaca
    36841 aataattttt ctatttcttc taatatccag tatttgttta aatgcatacc aaagcccacc
    36901 tattgacgat attttttagg agtgaatttt ttctttgctt taactttggt taaggcaagt
    36961 gcaagttgaa tggcatcgta taatttttcc ttatcttcat cgtctaaagg ttcaccataa
    37021 taatttgttt cagcatctga attcaagcct tcaagtaatt tttcagcttg taaagcaata
    37081 tcttgtttat ctttttctgt taaatcataa taatggcgtt tatcagtacg tcctaaaaga
    37141 taatcagttg atacattaaa gtaatctgca actttttgta ttttatctcc agatggagta
    37201 cttttattcc atctagacat gcttccattt ccgaaattta actttctttc aagttctgcc
    37261 aacgtcattg aacgttcttt taataagatt tttatacggt ctaatagcat aggatatgca
    37321 ttcctttcaa aataccgaca aaaaaagtat ttttctgcaa attatctatt tacaaataga
    37381 gaatttgcag ttatactagt tttgtaagca agatatttac ggcatacaaa gactaaaagc
    37441 aataaactaa tagtctcccc agacatcagt aagctcgctt tttgtgcgct gtattttgta
    37501 tgcttacata atagcatatt tgcagttttt gtcaaactta ttagacaaaa atattagaaa
    37561 atattcgata agggggtatg aagcatgact ttaaaacagc aagtattaat tgctttagta
    37621 aagaaaggtt ggagtcaaag agagttagct cgacgaatgg gtatatcaat tacttattta
    37681 cgagatatct tcatcgaaaa aagaaaacct aaggaacgtt taaaacaaat cgaagaactt
    37741 ttggatatca agttagaagt agattctaat gatcagccat cagaacagga ggcgtaaaaa
    37801 tggtacaacg tttgagcaaa gcggagttta aagagaaata taagtattct gaaagtactt
    37861 atcaaagaag aatgaaggaa tttaaaaaat cacgttttag tgaggggtat aacgcagtta
    37921 cttccaaaga aataacaatt gatgtcgatt tatatgaaca gtttagaaca tggcgttcta
    37981 aaaatcgatt tagagttaga gctgtttgtt aatagctaaa aaatctaaat tcaccacttc
    38041 tgaaaagagg agttgctgct ctcctttcaa gtttgccaaa gtccacgttt tttcattttg
    38101 gcaactcctc ttcttggagg tggtgacggc gagagacgaa ggagggatga catgaaggaa
    38161 gaatcaaaaa tagggatttt gaatgagaac aattacacaa gattaattat tgaaacagaa
    38221 gaagggaaaa aagtagcaga agttacattg accgatgcaa ctcctgctga cggttatcgt
    38281 attcgattaa ctcctaaata tgattaacct tttggtgggt gtggatcgtt tccgtgacta
    38341 tcacggcttt gaatttttcc atctctgcca tgaataaata gctcagatcc ttgattttta
    38401 gaaatctctc tagcgatttt tactgcttct gattttgtat tagtatgtgc agtcgcttta
    38461 gaatttccag cacctttgac attccaacca ccattggaat taggtacaac atgttgattt
    38521 tttcccatag ttttcacctc catttctata gaaatatttt atcaaataga aatggagaaa
    38581 ttcaataaac gaaaggagaa gtgatatggc atataccttg caacaagaac atcaaattct
    38641 cggtttgatt aaacaacgca ggaaacaatt acaagatgac cgtgcagcgc ttagaaaagc
    38701 cgatgagcta tcagataggc aagctgaact aattgcttct gaacttgagg atttgagaat
    38761 gctagaaata aaaaataggg agattagatt atgaagaaga cagacacact ttttatagga
    38821 ttcattttgg ggttattagt gattgtagcg caccaaagta ttatcggggg aagcttgttc
    38881 gcagcattga tggttttaat caatctgctt gattcaaaag aaaggagcaa ctatggcacg
    38941 agaagaagcg ctaaaaatcg gtaaagtgat tgctgataat cggtggggaa atagccgccc
    39001 tattatttta agcaagcaac atatcgaaaa gcaaaaagca tggcaacaaa taaaaaagtg
    39061 actctgccag ccagcaaaga gtcacataca aatttataaa actaggagaa ttatatcaca
    39121 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    39181 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    39241 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    39301 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    39361 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    39421 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    39481 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    39541 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    39601 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    39661 aagcaccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    39721 gaatggcttg aagaccgtaa gaaaggcatc ggaggttcgg atgttggaac aattttagga
    39781 ttgaataaat ggaaatctcc ttatcaacta tggctagaaa aaacaggaca agtagtactt
    39841 gaagaatcag gaagtgagcc cgcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    39901 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    39961 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    40021 gaatgcaaaa cagctaatca atttctcggt aaagaatggg aaggcgaaga ggtaccgctt
    40081 agctatctct gtcaagttca acattacatg aacgttctaa acaaagacta ttgttacatc
    40141 gctgtcttaa tcggtggtca aaaatttatc tggaagcgga ttgaacgaga tcaagagctg
    40201 atcgatacaa tcactgaaca attagtagag ttttgggaaa caaacgttct tggaggtatc
    40261 gagccaatta ttgatggtag tcaagcgaca gctgatttct tgaaagagaa gtatgcagat
    40321 gttgaagacg ttcaaacgac gttaccaatt catttcgatg aactagttga acagaaaaac
    40381 gaactcaaac ggactaagaa agaaattgaa tcagctatcc gtcaagtaga caacgagatt
    40441 atcagcgagc taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa catcatcagt
    40501 tggaaatttg tccgtacgag acgtatgaac tcgaagaaac tagcagagaa atatccagat
    40561 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaagggag
    40621 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagcaa agccacagaa
    40681 acaagttgca ccaggtcaat taggacttaa agctttgatg aatacaccaa caatgagaaa
    40741 gaagttcgaa gaggtgcttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    40801 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    40861 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    40921 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    40981 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    41041 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    41101 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    41161 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    41221 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    41281 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    41341 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    41401 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    41461 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    41521 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    41581 caaattacat cgaaaagtga tgaattcatt cgtttggact aatccttata tgtataaatt
    41641 atggaacttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    41701 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    41761 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    41821 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    41881 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    41941 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    42001 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    42061 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    42121 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    42181 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    42241 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcca atcgtgatta
    42301 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    42361 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    42421 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    42481 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    42541 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    42601 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    42661 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    42721 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    42781 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    42841 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    42901 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    42961 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    43021 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    43081 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    43141 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    43201 ataaaaccag tcaaaggagc gatcgcatga atttgactaa acaagaaaaa agaatctgtc
    43261 gtcagataat gttgcaaatt ataggtgatc gtgacatgga cgaattatct caacaagaga
    43321 gtcgaatggt tcggaagata agtaagctaa ttatttatcg ggatacagaa agatttctca
    43381 ctgttttagg taacgtctac gagaatccag agttattgga ggtagagtaa ttgccaaaaa
    43441 tattagatgc atgttgcggt agtagattgt tctggtttga taagaacaat tctgacgtaa
    43501 catttatgga ttgtcgtcag cagtatgaag aattatcaac aggtcatgtg atcaatgttg
    43561 atccagatgt ggtcgcagac tttcgcgata tgccttttga agatgatgtg ttcgatatgg
    43621 ttgtatttga cccgccacac ttaagacatg tcggcgaaaa ctcatggctt gccaaaaaat
    43681 atggaaaatt agacgaactt tggccagaag acatcaggca aggttttgca gagtgtatgc
    43741 gagttttacg accttcagga acattaattt ttaagtggaa cgaggaacag ataccacttt
    43801 ctgatgtgtt agaggctatt ggagaacagc cattgtttgg taataaacga agcaaaacac
    43861 attggcttgt gtttatgaaa aaattcagct aacggaggaa acaaaggagg aaaagtaatg
    43921 aagattgtat taaataaatg ttttggtggc tttgggttga gccatgaagc aaaaatggaa
    43981 atttttaaaa gaaaaaatat tgaagtattc ccatacatca ataactttag ctatgactca
    44041 gatgatgaat acacgagata cacaggccaa aagctaggtt cgatggattt tatctactat
    44101 ttcaaaaaag acccgaaaat cgacaaagtt acaggaattt atagcgaaat tgaccgactg
    44161 tacggaattg ctgatgattc cagtttttca agtgattcaa acagaggtga taaggacctc
    44221 gttgctgttg tcgaaaaact aggaagtgag gccagtggac catatgcaag tcttaaagtc
    44281 gttgatatac ccgatggggc tgagtgggaa ataagtgatt atgacggcgt tgaaactgca
    44341 cattatggtt ttcaaacagg tagtatttag tccactaatg acaactaaag aggaggcaga
    44401 aggatgagtg aagatgaata ctttgaacag tagtctatta acgacagaga tttttaactg
    44461 gaaaggggtg tagtcatgga cgcatcggaa gtagtaaata aaattaatag tctcaaagag
    44521 gttttcggag acgtcgaagt agtgataaac gcacaaggct atgacaacgg attttttaag
    44581 cagattaccg acattagcat tcagcaagga atggaagacg aagatggaaa ctttatcgat
    44641 gaagtagcca ttttagtgac ttgcgagtaa gtaattgaca gctaaagagg aggcagaagg
    44701 atgaaaaaat tgcaccaact catatctgag aaagaaagcg aactccaaaa tttagaagat
    44761 agtttgggat tggggtttcc aattgttgag caagctaaaa tgactcaaat cagtcatcta
    44821 cgacttgaat tggaagattt aagacaaata gaaaaatcaa ttcaactcaa cgacaaccag
    44881 aaaatcgtgt tggagtggtt gaaaagcgag acaattttaa ctagagaagc accaatatta
    44941 tctgttaatg ctttttctga taaaaattta ctaggaaaat tacctgataa agtacgcaaa
    45001 gcttataaac tattggcttg taaacaagaa tatgaagtcc tatcagcatt cgctcaatgg
    45061 ggattagaac aggaggaagc ggaatgaaca acagacatcg cagaataaca aaactaagaa
    45121 aacaggaact gaatgtacta aagacaaagt ttgaaaaaga atatggaatt tcagcagaag
    45181 aaacatataa agtggcaagt cagtttgttg ctgatgcgag taatgctatt cgtaagtttg
    45241 ggatttcgat attaaatgat gatcgtaaat gggaggaaat gagatgaaaa taaaagacgg
    45301 attttacgct agtagtcatg gtatcggcgg tttaatgcta gacatgccga taaagaaccc
    45361 taaaacacgt cagaaatcaa aattcaaaat cggtgacatg gtccgatgta aagcagaaga
    45421 gttcatctat ccgtttcgtg gatatgtaga gcatgtctat aatcattcag caatcattca
    45481 cattgaaaac acgatggaat gcgataagtg gttagcgaaa agcaaagata atttagcagt
    45541 ggctcgtttg gtggatatgg aactaatcaa tgacaaataa aaaagccgga tcgctccgac
    45601 tgattcaata aatccaacac atttattata tcacataaag gagcggtttg acttgatgca
    45661 attgttacga gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa
    45721 ctttcggcgt ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat
    45781 tacggatatg ccgaaggcac cgaagcacgg caataaggca gaagacgcga tcattcagat
    45841 gatggatata gaagcggaga gagacgcaat actagcggct ttgatggctc ttagtctgat
    45901 tagccgtcag atactctact acagcttctg tgacgtaaac aagcactcta attatgaaat
    45961 agggcaattg atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt
    46021 aatcgaattt gcagaagcat acaaaaaagg cgtgttagtt cagtatcgtt gattttgtag
    46081 ggtttttgta gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc
    46141 gaaagattag tgataggtct gagacaaaat aaaatgtaag ggaggaaatc tccctcatcg
    46201 ttttaactta agcttcgata gacagcaacg gaaatattaa gaataaggat gtgaatttca
    46261 actccttcta aattgttctt attatctatc atccgttgtt gtctattaat ttatgcatcg
    46321 gaggaaaaac gaatggataa agaaatcaaa gtaactgtca aattagattt gactgaacta
    46381 aaagaactgc tcaacaaggc tagtgaccaa gtcgaacagt tacaagaaac tttagatgaa
    46441 attgctaatt ttaaaatcca agtttcttag cagtatattt tttagcagct gcactcataa
    46501 aatcatacca agtatcagaa aaatataact taactaaata attaaaaaag gtgggtaaat
    46561 agaaatgacg gcgttaataa tttcaatttt tgcgctctgt cttaatgtct atatgattgg
    46621 atttaaaaat ggacaaaata aaaaatagta gcagccgaga ataattttat agtgtcactg
    46681 tggcggaaat agaagacgca aaggtggagg taggaagcgt atggcgcact atcgggacct
    46741 tgtacaaaac tagaaaggac tagtacgtgt gtggtgcgag ccctagggag gaacaggata
    46801 accgcctgtg tgtaggttgc tattacatat tagatcactc tttgagtggt ctttttattt
    46861 acataaagga ggctacataa tgagaaacta ctggtatata tcgctaacta atgaatatcc
    46921 tcgaaccatt gatgattgtt cagtgcgtgt tgtgcgttct gtccaaatca aaaagaagta
    46981 ctccattgtt gaaatgacca gagaagctac gccaaaagag attgataaat gcaaacttct
    47041 ttattgcggt catggtttct atgatgaacc aaacattcaa aataatatta acaagaattt
    47101 gagggattag aatgcaagaa atggcttaaa aatctccaat caggacaaat caagttagac
    47161 aaagtttccg atttaaagat attgattgaa gctgatttaa tgctaaaaga tattgaaaat
    47221 tagaaaacaa aactcaacct aagaagattg cgaggtggtg tgtattgaat ggcaagacaa
    47281 cgtgatccaa gacgtgatga agccaaaaga atttggttag aatccaacgg agaaaagcag
    47341 ttaaaggaaa ttgcatctga attaaatgtt tcagattctc aggttagaaa atggaaatcg
    47401 caagacaaat ggagcgctga attgaaaagt aacgttacca atggcaaaag taacgttact
    47461 aatcaaggtg gcgctcctat tggtaatcaa aatgctaaag gtaacaaagg aaacagcaga
    47521 gcatcaccgc caaagagaaa taagaatgct cttaaaactg gtgagtatga aacaatattt
    47581 tttgacacgt taaccgataa tgagaaggac atctattcta gtttgaatga tgatccttct
    47641 tttgctttgt ccgaagaaat acgacttctt aaaataagac aacttcgaat gatgaaaaga
    47701 atccaacaag ctgaagctgg actaaatgat gaagaagtcg aacgattgca gcagctaaga
    47761 aagattaaaa ctccgattga aaaaaatggt aaaaagctag aaatcaagcg tgaggttatg
    47821 caagatgtgc agattagcag aaaaaaacat cgcaaaattg atgatattct ttcaattgaa
    47881 gattcattga ctcggattag caaccagtta gctaaagcca tcaagcaaat gaatgaactt
    47941 tatatgaatg aatacagaac tgatttaatt aaagctcaga ctgataagat ccaagctgag
    48001 acaaatgaaa ttggcggaaa taattcaggt gaagaaatag aagaatggaa acaggcagtt
    48061 ttaaatgccg caaacaaacg ggcggtgaaa gaaaatgaat aatgaattta ttccttttgc
    48121 tgatattggt tctgccattg attattacta tgataaacca gtagcttttt gccaagatat
    48181 tttgcatttg aatcctgatg aatggcaaga aaatgtttta aatgatttag ctgaattttc
    48241 aaaggtttct gttcgttctg gtcaaggagt tggaaaaaca gcattagaag caggagcaat
    48301 actttggttc ttaacgtgtc gaccctacgc taaagtaata gcaacagctc cgacaatgaa
    48361 gcaactttac gatgtacttt gggcagaggt agctaaatgg ttaaatgata gcttgatcaa
    48421 aaacttactg aagtggacaa agaccaaaat ttatatggtt ggtgattcag agcgttggtt
    48481 tgctacggct agaacagcga ctaaaccaga aaatatgcaa ggttttcacg aggaccatat
    48541 gttgattgtg gtagatgaag cttctggtgt gtctgatcca attatggaag ctattcttgg
    48601 tacgctatca ggttttgata ataagctgtt gatgtgtgga aaccctaata atattgaagg
    48661 tgtcttttac gattcccaca attcagaccg tgataaatac agagttcata aagtatcaag
    48721 ctatgatagt aaacgtacaa acaaagacaa tatagaaatg attcttaaaa aatatggaaa
    48781 agaaagtgat gttgctcgtg tccgtatttt tggagaattt cccaaaggtg cgttggattc
    48841 atttatcagt cttgaaacgg ttgaattggc tacagaaaaa caaattagtg attctttagt
    48901 caataaaaca acggttgctc atattggtgt tgacgtagct cgatatggtg atgattctac
    48961 gattctcttt cctagaattg ctaccagggc attggagtat gagaagtatt caaaacgtag
    49021 cactatggaa acaacaggat atgtcatcaa catggccaag aatctaatga gtcaatatcc
    49081 gagtattgat aaagtgatga ttaaagtcga tgacactggt gtcggaggtg gtgtaaccga
    49141 ccgcctagaa gaacttatag aagacaagca ttatcctttt gaggtgtttg gagtgaataa
    49201 cggttcaaca tcagaagacg atttttacga taatttaggt actcaactat ggggaaacat
    49261 caaggaaatg ttagaagaaa atatgacagc aaatcttaac ggagaacagc ctgttattga
    49321 attgccttct gatagttcgt taatcaaaga attaagtact cgcaaattca aaatgacaag
    49381 tagaagtcgt atacgtttag aaagtaaaga tgatatgaaa aagcgaaata ttggtagtcc
    49441 cgatattgct gacgcactgg ctttagcgtt ttatgagcca ccaagtcact atcaatttat
    49501 tcaattttag gaggtgagtt tttgattagt acagtattat ctttagagag atataagaag
    49561 ctacgtgtta aatatgcaac acaaattgaa gatggaatgt ttgatccgaa tggttttata
    49621 gaatatatga aaccattttt tgctgatcgc gaaagaaaat atctagctta tactagcgaa
    49681 aagaatgaaa tagacaatag accaaagcct aacactgata ttgtaaaagt taataataaa
    49741 cttcatgctg gtatgtattc aattgtcgta gaccaagcag tcaatcattt tactggtata
    49801 cctatcaaat gggattacga tgtatcggaa caaaaaagaa cactcattca aagattaaaa
    49861 gataaattct taaaaaatga tatagaactt ccaacagttc cagaagcttt taacaaatta
    49921 acaagcaatc ttgattccat gagattcgca atgcttgatt ctgaaactgc aacttttcaa
    49981 ggagcttgtg gagtagcttt ccgattgtta gagcctgtgg aagaagatga tggttggaag
    50041 ttaagagcaa gtaatattga accttggaga gcagaaagat atggaaatgc cggaatctat
    50101 atcaaagaaa agtatgactc ttaccagaaa aaattttttc aagaaatgaa agttatgaca
    50161 agaaataaaa ttctgacata tgcttgctat ggtgatttga attttgttac aagcggaacg
    50221 ttcaaaaaaa ttgatgagac cgataaccct ttggggacga ttaccttggc agaatttaaa
    50281 aataatacga atcgttattg tgattttgaa gtagctgagg aaattggaga tgcaattgat
    50341 cgtgctttgt ctgatcaaca aaacgaaatc gaacaattta agcttgcgta catgcttgtt
    50401 actggcacaa caatgagcaa aggcactgct aaagaaatga tgaatcagct agggattatc
    50461 aacttaaaag accctactgc aaaagctgaa tatgtcacaa aaaaattagc aaaagatttc
    50521 aatgaatatc atatggatct attgaaaaaa cagttttaca ccatttgtaa agcaatcgat
    50581 tttaacgatg aggtgtttaa atcaaacagt tctggagaag ctcgcaagtg gcaaatcatt
    50641 agcctagaag cgaaaacaaa tactaaagag caatatttta gagaaggatt gaaagaatgc
    50701 gcagaaacaa ttgctgcttt tcttaagttt catgacaaag tagaaattga gcctgaaaaa
    50761 attattttta ctttctctag atctttgcca actgatttaa gttatttagc tgaagcttta
    50821 cctaaacttg caccatacgt atccaaacga actattcaga gccaaattcc atttgtaaca
    50881 gatgttgatt atgaaaatga aatgatggaa ttggaaagtg gaagtgctta tccagatagt
    50941 gaatacaatt ttggcggagg tggcaatagt gacgatagaa acgaagtatt ggaccaaacg
    51001 tcgagaactg gaggatcaag caaggctcaa acaagaaaat cagacactta aaaaattaac
    51061 tagtgtattt cctgaagcac tgaaagagat acaagcaaaa ctattatcac aagctgactt
    51121 acacaatatc acttatccag aaatgatgga gttttacagt acaagtaatc agaaaaaata
    51181 tcgtgaatat gtggaaaaaa attataagtc attaaaaatg tatgatgcaa aatacaaaga
    51241 gtttatcgat gaattttttc caccatttga ctatgcaaaa gtcaatcgct tattacaaat
    51301 acgatcagat gtatttaaaa ttcttgcaga atatgcgatg gatgcagatg tgaatcaata
    51361 tttttctgat cgcttagagg aaattcttca aagaacatat tcttctaatg ctaatgtttt
    51421 tgttcaactt ttaaacgttg atataccaaa ttatttacca gaaaatgaaa ttaaacatta
    51481 tttgaactat ccatggtcag gaaagacatt ttcaagaaga ctttggggaa atatttcatc
    51541 gcttgaacag aaactctcta atgctattgt aaaaagtgtt gctagtggag aaggcgttat
    51601 acacgcatta aacaccatga gattagactc agaaatttgt gacatgttta agttagaaga
    51661 atcaaagtat aacaaagcga tagaaaatct cgttcgaacg gagtatgcaa aatttgcaca
    51721 agatggtatt gaaaaatcat atttagaaac aggtattgag gaatacaacg tattgactgc
    51781 aaaagatgag agagtttgcc ggatttgtgg aggaaaggca agtaagaatc cctataaact
    51841 gaaagatgct gtcataggtg aaaatcgagc acctttccat agtcgttgta gatgtacgga
    51901 tgttcctaat ttaccaaaat taggaaagga tattgatgaa gaatatgacc gtttatttgg
    51961 cgatttatta gatgagtttg cacatgattc ttttggaatt aatttgaaac ggaggaagta
    52021 gaatgaaaga tttttttgaa gcagtactaa caattaatgt aaatgctgat attgcagaag
    52081 cctacaaaac agctattgag tctgagaacc atcctaatgg cttgagagac cattggaatg
    52141 gcaattatgc ctacgtggtt attggcgatc aaactgttaa ttatcaagat aatactccag
    52201 ttgataagaa taccgttaat ttaacgattc aattattatc tcattcatta ccaaatttaa
    52261 aagaaacagt tgattggtat gaaaagatgg gatgtattgt tgttagaact gactacaaag
    52321 aaggaaagtc tagtaattag gcttttttat tttgtccgaa atgacgttaa actagcgcaa
    52381 tactgggctt ggttgaatgg tggggcgcaa ctattaaaac tcaaagcaat gcggggcgtg
    52441 aaaacgaatc gtggggcgaa aggagaatga gcatgaaaca caaatcatta atgccaatga
    52501 atttgcaata ctttgccgaa ggtgacgatc aaaagttttc ttttgatgac ttcaaatctt
    52561 ttgtggaatc caatgaagaa gctcaaaaat ttattcagtc acaatctcaa agtgttgccg
    52621 acaaacaatt ggaagcttgg aaacaaaata acttagataa aattaaacag gataccatca
    52681 aggaatatga ggaatctaag aaaaacaagt cacctgagca aattcaactg gaaaaactac
    52741 aagctgaatt tgaagcagaa aaagcgttgc gtgtgacaag tgataataaa gcatttgttg
    52801 cagaacaaat tgctggatta gaactagatg gagagttaaa agagtctatt tctcaattta
    52861 tgctaaataa tcttgttagt tcggatacag atttcactaa gaatgctgtt gaaggtttta
    52921 caagtgtttt gaatgcaatt aatgagaaac atgcagatgc attaaaagaa ctacaaatga
    52981 agtctgcatt tggtggaact caacaatcga ataaccaggt tcagcagaac aatgaaacat
    53041 taacaaatcc agaagaacaa ttaggacaaa ttcttcaaca atttaactag gagagtgaaa
    53101 aattatgaaa aaaacatctt taaataattt agagtatttg gatatttcac cagcgattaa
    53161 tgctatgcaa gtaccaaata cacctttttt aagctattta cttggtgctg gaaaaacaga
    53221 gccagcaaac tcgacagaaa ttaaatggcg tgaatatgat atcaacaacg atgattcttc
    53281 tgaaaaactt gagggcggag aatatccaga tgctgaatca ggtcgaactt ggtttaacaa
    53341 ctatactgaa atttttagaa aatcaacctc tgtatctggt acattagatg ctattaatgt
    53401 gaatggagtc ggaaatgaat taactaatca agtagcacta cgtggtatgg aaatgaaaat
    53461 tgatttgaac cgaaaattga ttactggtgt aaaagctgat gaaaatggtt ctaaaggtcg
    53521 tcgaatgaat gggattttga acttgatcaa ttcagcaaat aaggcagaaa cagccactgc
    53581 gggtgcagta acaagaaaag atatcgatac tttatttaaa ttgatgtatg aaaaaggtta
    53641 tatgggagaa aaactatgct tgatttctcc agatatgcag gagttaatga ctgatgagtt
    53701 agatggaaaa tcaacaaaaa ttgttcagtt cggggaaaga gtaacttttg gattgcaaat
    53761 tggaaatatc gtgtctaatt acggtacagg tattgctcta ctagaaccat cattgccaaa
    53821 aggaacaatt gccgcaatcg atactaatta tgtgaaacta cgtccattac gtgaatggag
    53881 agcagaagaa cttgcaaaaa caacggattc tagacgtatc ggtcttgtag gtgaatactc
    53941 tcttgaatac aatgcttcaa actctggggc aattttaaat ttaaagtctg aataaaaggg
    54001 agttagtact cccttttttg ataggaggaa ttacagtggt aaaaaaagat gaaactaaaa
    54061 aagatgaagt cgtgaaatat agagtaggta aaactaaaaa ttttgttggg tttgttcatc
    54121 ctaaaactcg tagatttatc acagcagatt caaataacga atttatcatt tctatagatg
    54181 ataaagaagc aattgcaatt ttggaagatg caattgatgt taatgaaatt taggaagtga
    54241 tctgatggat gaatcgctaa aaacggaaat cattgagtct acaaaagaag attttccaga
    54301 tttgagtgaa gaacgcataa ctaatttatt agaaataatt ttgctagaaa ttgaatcata
    54361 caacacttgt aaaaatgata tttcatggga aaagttaaaa agcgtgatta gtgaagtgtt
    54421 gtataaaata ataaaaaatg aatcagaaaa aacagtatct tcagttagac gtggcgatac
    54481 aacgattagt tatgcttcaa caacaaatga tgttagtgag ttacttctag gctatggtga
    54541 tttgatacga agagttattg gttgtggagg attggagttt ttttaatgaa tgaagcagat
    54601 attttagaaa ctacttatga agatagttgt attattgaaa gactgacgga cattgaagat
    54661 tctaatacaa atattactat tcaagattac aaaaaagtat atgataatcc tatttcttgc
    54721 gctctttcac aaggtcaaat cgatggacta gcagtcatag aagatggaga gatggtaaat
    54781 gtttcaactg acacatataa attatttgtt catcctaaga ttaaactaaa aaaaggagat
    54841 cgaataacaa taactcaaaa ggccagtggc ttaatttttt ctctatttgc tactaagcct
    54901 ttttactatc ctagtcattg tgaagtgaat ttgataggaa gtgagaaaaa tgggtaatct
    54961 caaatatgaa tcaaatgcag aaaagatcat tgagaatttt aaaaatatga ctgtaattgc
    55021 tcaaaaagaa ggaatatcct tcgttaacga ttcaatgaat aaagtcgtta gtttagctaa
    55081 accattaact cctgtaaaat caggtaattt aaggcgagga tatcgtgtag taaaagctag
    55141 aaaactatca agcggtcgaa ttgttggagc agttataaac aatgaacatt attttaaata
    55201 tgtagaagaa ggtcatagga ctaaaaatgg tggctttgtc aaagggaaat tcatgttaac
    55261 tcgtgcaacg aatcttgcaa atatgtctta tattcctcga agatttaaac aaatggcaat
    55321 aaaaatcgtt aagaaaggaa agtaacatga aagatgaaat cattgctgca atcagtagca
    55381 aattaaaaga aatctatcct gatgggacaa tatatcttga ttcagttatg cagtcaacta
    55441 aagattttta tttcgtttta tcagtaatgg aatctggaac tgaaaatgta ggaattgata
    55501 ttcaaaatgt ttctttctta attgatattg cattgattga taataaacct aatagaaatt
    55561 taattaatga attagtctca cgttgtggga ctttttttaa tacgattaca atcgatgagc
    55621 aaatattatt tccaaaagcc tatttacctg atgaagcaga tggtgttcaa catattcgtt
    55681 ttacattaga atttccacaa tatattgaat ggagtgaaag ataaatggga gaaaaaagaa
    55741 gtaaaaccgg aattatttct gtcgaaaaac ctacttggtt tcctttggaa gatgagacag
    55801 ggaattttcc ggtatatggt actgcgttgc caatgggaac agcagtaagt atcaaaccaa
    55861 cagctaatta tgaaacaaca caagattatg gggattcagt ggttcaagat caatttacag
    55921 cttttggtgg tgcagaagtt gaactggaag caaatggata tactcataaa gttttatcta
    55981 caattactgg agggaaaatt gttaaaggtg gtgccttgcg ttctggagaa gatattgcgc
    56041 aagatggtgc gtttgcatat agacgtaaaa aatcgaatgg gaaataccgt tatacggtct
    56101 tttataaagg acagtttgct ctagattcag atgaaacatc aacgattgaa ggaagtaaag
    56161 taagttttac tcatccaact tggaagggtt catttgtaga tgtaccagga cttggatata
    56221 tgtattcagt tgatgaagat gatgaaggcg tggataaagc aatgatcgga aattggttta
    56281 caaaagtagc aattccaatt gaagaagcag aactttcagg aggtacagaa taatgtcaaa
    56341 atatcaaaca acgattaaat taacgaaaaa agatgaagaa ggtaaatatg agcaagtaca
    56401 atttaaatct gctgaatttc taccaggccc agtcgtagaa gaagcagcag aagtaatgga
    56461 gggaatgcaa aatgctgtag atggaaaatc agtatcagaa gcattaagtc gagcttattc
    56521 atttattgca gatattttgt ttgaaggaca gtttactggt gaagattatc gtcaagggat
    56581 cgatgctcga gaaattgctt cgttgacagg taagttatta aaatctgtta ctgcaggctt
    56641 tgatgaaact tatacagaaa cgaaaaaaaa gtaagcgaag ctctcaaata tccttttttt
    56701 aaacattcga ttacttatcg agaattagat ttaaagacgc aattactcga agcaggttgg
    56761 acgttaccag aaattgaacg tactgacttt gacgagttaa tgcgtctttt tgcttttcga
    56821 gatgcagtaa aagagcatga ggatgttgag tactacgata acttcaccca attttaggag
    56881 gtgatacttt gaataacgat gacctaattc tgaagatgat actagatgaa tctggtttta
    56941 ctgccggtat gaataatgct gtcaaaaagt tgaactcttt tgatgaaaca atcgaaagaa
    57001 aaagtagaaa tagtggcagt tcattaggta gtatttggaa aatatttgct ggaagttttc
    57061 tagctagcgg agtaactaga atcgtaggag ctggctttga tctaatcaag ggttccataa
    57121 gtggagcggt tgatcgagta gatacgatga ataacgctct acgaaatttc caaaatatgg
    57181 gatttagtaa ttcagaaatt atgaagaata tcggaaagaa tgggctttta tctcaaggta
    57241 ttcaaggact tcctactgct ttgaatgatg cgataagtca tgttcaactt cttgcttctt
    57301 ctacaggtga tttaactcgt tcaactcaaa tatttaaagc tttaaatgat ggaattcttg
    57361 gtttcggtgg ttcaactgat caagttaatg aagctgttat tcaattatct caaagtttct
    57421 caaacggaaa agtagacgca caaacttgga actcaatgat caacgctcaa cttggaccta
    57481 ctctttctgc tattgctaaa aagatgggaa ttacaatggg agatctgaaa gaaggtttat
    57541 ctcaaggtaa gatttctgtt gaagaattcc aaaatcaatt aatagaaatg gataccaaag
    57601 gtggcggagg acttaaatca ttaagtcaaa tcgctaaaga ttctactaag ggaattaaaa
    57661 cctctataca aaatgcaaaa acagctgtta cacgtggtgt tggtgaggta atagaaggat
    57721 taaataaagc attagtagat tcagatttag gtggatttaa gggaattatt gataaagtgg
    57781 gcagttcaat ggaatcgttc ttaaaagtaa ttgctgcaaa tattcctaca gcagtatcat
    57841 ttttaagtaa tttatttaac gaaattcaaa aatttggttc tgctttgaaa ttcatgatgc
    57901 cctttcttgt tcctgcagct accgcttttg gagcgttcat gtttcaactt aaaggtatac
    57961 cagcaataat aaaaagcttc aataatttta agaatgccat aattggcgtt ggaaattcat
    58021 taaagattat gggggcaata gctgctgcta attcatttgt tttaattatt ggtgcagtcg
    58081 ttggtgcaat agctgttttc ggttacttta tggcaaccaa tgaagagctt agaaacaagg
    58141 ttatatctat ttggaatgat gtgaaagatt ccgtaattgg tgtattaaaa aatataaagg
    58201 actggggaat tgatacttgg aattctgcta aataaatggc atcaaatgca gttgagggtg
    58261 tcaaagatgc ttggtcagga acaaaggaat ggttttctaa cacctggaaa ggtattaagg
    58321 atggggccac aggtttattt gataaaacag tagagacttc aagaaatgca gtcgatagtg
    58381 taaaaaatgc atggtctaat atgaagaaat ggttttctga tacttggcaa agtataaaag
    58441 attcagccag agaaaaatgg aatggaatca aaggttctat tatgtcagtt gctggtccat
    58501 tgattacagg tataaagaat gcatttttac atgttacttt ttatctagag accttatgga
    58561 ataatttagt tgagattggt aaaaacgtct ttgagatttt aaaaaatgtt atacttgcgc
    58621 cggttttgtt tattacctcc atgatttctg gtggatggga agaaacaaaa aataatatga
    58681 ttgctgtttg gaataatatt aaagaaagtg ccataaatat ttgggaatct ataaagaata
    58741 tatttgtaag ttattttaca aatatttatt ttgctgcact caatatttgg acagggttca
    58801 agcttacatt gataaatatt tggaatgaag tggtaaatca agctaaatcg atttggttaa
    58861 acataaagta tttttttatt aatctttgga ttgatataaa atattttgca attcagaaat
    58921 ggattgaatt gaagttttct attattcaaa cttggatcga tttaaaatat aatgccatta
    58981 ctacttggaa taatattaaa cagtttttca aagatacttg gaaaaatatt aaagatacgg
    59041 catacaatac gtggatttct ataaaaaata ccatgattaa tacatggaat aacattaagg
    59101 actctttctg gaatattgtt actggaattg ttaattctgc tgaaaatgca tggacaaatc
    59161 taaaaaatgg tgtttcaaat gctattaatc gggtaaaaga aatctttaat tcgttaaggg
    59221 aagtcaattt atttgaaatt ggcaagaaca tcattgatgg acttataaat ggtgtaaaag
    59281 aaaagtggaa tgctttgaaa gatactatca aaggtattgc aggtaatatc aaaaattgga
    59341 ttaaaggagc tttagatatt cattctccat caagatggat gcgtgatatg gttggtaaaa
    59401 atattgttca aggtattatc gttggtattg ataaagagca aagtaagtta gatcaaacca
    59461 tgacaaattt agtaaaaaca ccgtctgttc aacctgtagt aaatggaata agtcacctac
    59521 caattacaca aacaaagcaa aatatatctg ctagtgaaac aaaagaaatt catttacatt
    59581 taaatgttta tggagattta cctgattcaa tggttcgaaa gattactaca aaaattaaaa
    59641 cagaattgac aagacaaatg aaacgagatg ctggtgcagt aggaggaaca ttatatgcaa
    59701 cttaagagag gacaattttt tattaatcaa cattattctt ctgaatttaa tgtgtatatt
    59761 caaaacaggc ctgcttctgt ttcagctagt cgagtaattg aattgagaga gcgagaagga
    59821 aatgattcta tcattattga taaaggctat tacaaaaatg tgactagaaa gattgagtgt
    59881 tactataaag caccttcaat tgatatggtg caggagtggg aagatcgaat cactgaatgg
    59941 ttagatatgt cgtcttatag tgattttatt ctttactatg atgaacaata tatttatcaa
    60001 gctgtagtaa tagaaggacc tgaatttaaa ggaacaagaa aaacaggaaa tattgtccct
    60061 tttgaattta cagtgagtat tcgaccattt aaggaaaatt acaatggtag atttactatt
    60121 cgacaaacag aaacttttga aatttataac ccagagaagt attcttcaaa accgcttatt
    60181 aaattgagtg gttctggaga tgcttctttt tatattaata aggataaata tgatttgaaa
    60241 tcgttggaca gagaattatt tatagattct aaattagaag aggcttatcg aaaattagat
    60301 ggtaatttag aacatcagga tcaggtcacc ttatttttag attttccatt tttatatcct
    60361 ggaaaaaatg aaattaaatg gacgaaaaat attcattcat ttgaaataat gcctaggtgg
    60421 tggagaaaag tatgaaacca agaatatata gtcctactga aacagatttc tcaacgaatg
    60481 gcttaggaat tttaaaggat tgtacaagat gtgaaatata tgaagtagct aatggaaaat
    60541 atgaattaga gttggattat cctttaggaa ctagatttga tgaatatttt gaaaatgact
    60601 atcaaataaa agcaaagcca a
//
LOCUS       DAYYHS010000007        57168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000007 DAYYHS010000000
VERSION     DAYYHS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.007"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..315
                     /locus_tag="ACHA6A_000409"
     CDS             85..315
                     /locus_tag="ACHA6A_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8968646.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            412..1713
                     /gene="murA"
                     /locus_tag="ACHA6A_000410"
     CDS             412..1713
                     /gene="murA"
                     /locus_tag="ACHA6A_000410"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8968647.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1726..1902
                     /locus_tag="ACHA6A_000411"
     CDS             1726..1902
                     /locus_tag="ACHA6A_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8968648.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1899..2033
                     /locus_tag="ACHA6A_000412"
     CDS             1899..2033
                     /locus_tag="ACHA6A_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968649.1"
                     /translation="MINKNHSGSSRYEVSHEAEHDKRKQKGTLTLVAIPFFSKRKEGI
                     "
     gene            2037..2333
                     /gene="yidD"
                     /locus_tag="ACHA6A_000413"
     CDS             2037..2333
                     /gene="yidD"
                     /locus_tag="ACHA6A_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8968650.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2518..2985
                     /locus_tag="ACHA6A_000414"
     CDS             2518..2985
                     /locus_tag="ACHA6A_000414"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8968651.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3070..3954
                     /locus_tag="ACHA6A_000415"
     CDS             3070..3954
                     /locus_tag="ACHA6A_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8968652.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4068..4304
                     /locus_tag="ACHA6A_000416"
     CDS             4068..4304
                     /locus_tag="ACHA6A_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8968653.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4392..4472
                     /locus_tag="ACHA6A_000417"
     tRNA            4392..4472
                     /locus_tag="ACHA6A_000417"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4426..4428,aa:Tyr,seq:gta)
     gene            4479..4550
                     /locus_tag="ACHA6A_000418"
     tRNA            4479..4550
                     /locus_tag="ACHA6A_000418"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4511..4513,aa:Gln,seq:ttg)
     gene            4675..4845
                     /locus_tag="ACHA6A_000419"
     CDS             4675..4845
                     /locus_tag="ACHA6A_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968654.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5065..5772
                     /locus_tag="ACHA6A_000420"
     CDS             5065..5772
                     /locus_tag="ACHA6A_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8968655.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5831..6475)
                     /locus_tag="ACHA6A_000421"
     CDS             complement(5831..6475)
                     /locus_tag="ACHA6A_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8968656.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6601..6888
                     /locus_tag="ACHA6A_000422"
     CDS             6601..6888
                     /locus_tag="ACHA6A_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968657.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6996..7316
                     /locus_tag="ACHA6A_000423"
     CDS             6996..7316
                     /locus_tag="ACHA6A_000423"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8968658.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7397..8710
                     /locus_tag="ACHA6A_000424"
     CDS             7397..8710
                     /locus_tag="ACHA6A_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8968659.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8707..9426
                     /locus_tag="ACHA6A_000425"
     CDS             8707..9426
                     /locus_tag="ACHA6A_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8968660.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9554..10105
                     /gene="raiA"
                     /locus_tag="ACHA6A_000426"
     CDS             9554..10105
                     /gene="raiA"
                     /locus_tag="ACHA6A_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8968661.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10327..12861
                     /gene="secA"
                     /locus_tag="ACHA6A_000427"
     CDS             10327..12861
                     /gene="secA"
                     /locus_tag="ACHA6A_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8968662.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12987..14088
                     /gene="prfB"
                     /locus_tag="ACHA6A_000428"
     CDS             join(12987..13058,13060..14088)
                     /gene="prfB"
                     /locus_tag="ACHA6A_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8968663.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14193..14879
                     /gene="ftsE"
                     /locus_tag="ACHA6A_000429"
     CDS             14193..14879
                     /gene="ftsE"
                     /locus_tag="ACHA6A_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8968664.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14872..15756
                     /gene="ftsX"
                     /locus_tag="ACHA6A_000430"
     CDS             14872..15756
                     /gene="ftsX"
                     /locus_tag="ACHA6A_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8968665.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            15908..17356
                     /gene="cls"
                     /locus_tag="ACHA6A_000431"
     CDS             15908..17356
                     /gene="cls"
                     /locus_tag="ACHA6A_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8968666.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17693..18550
                     /locus_tag="ACHA6A_000432"
     CDS             17693..18550
                     /locus_tag="ACHA6A_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8968667.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18665..19585
                     /gene="pstC"
                     /locus_tag="ACHA6A_000433"
     CDS             18665..19585
                     /gene="pstC"
                     /locus_tag="ACHA6A_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8968668.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19585..20469
                     /gene="pstA"
                     /locus_tag="ACHA6A_000434"
     CDS             19585..20469
                     /gene="pstA"
                     /locus_tag="ACHA6A_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8968669.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20484..21290
                     /gene="pstB"
                     /locus_tag="ACHA6A_000435"
     CDS             20484..21290
                     /gene="pstB"
                     /locus_tag="ACHA6A_000435"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8968670.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21306..22064
                     /gene="pstB"
                     /locus_tag="ACHA6A_000436"
     CDS             21306..22064
                     /gene="pstB"
                     /locus_tag="ACHA6A_000436"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8968671.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22077..22754
                     /gene="phoU"
                     /locus_tag="ACHA6A_000437"
     CDS             22077..22754
                     /gene="phoU"
                     /locus_tag="ACHA6A_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8968672.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23331..24023
                     /locus_tag="ACHA6A_000438"
     CDS             23331..24023
                     /locus_tag="ACHA6A_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8968673.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24020..24787
                     /locus_tag="ACHA6A_000439"
     CDS             24020..24787
                     /locus_tag="ACHA6A_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8968674.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24887..26455
                     /gene="liaX"
                     /locus_tag="ACHA6A_000440"
     CDS             24887..26455
                     /gene="liaX"
                     /locus_tag="ACHA6A_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8968675.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26752..27072
                     /locus_tag="ACHA6A_000441"
     CDS             26752..27072
                     /locus_tag="ACHA6A_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8968676.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27072..27422
                     /locus_tag="ACHA6A_000442"
     CDS             27072..27422
                     /locus_tag="ACHA6A_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8968677.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27589..28527
                     /gene="hprK"
                     /locus_tag="ACHA6A_000443"
     CDS             27589..28527
                     /gene="hprK"
                     /locus_tag="ACHA6A_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8968678.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28542..29372
                     /gene="lgt"
                     /locus_tag="ACHA6A_000444"
     CDS             28542..29372
                     /gene="lgt"
                     /locus_tag="ACHA6A_000444"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8968679.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29416..30441
                     /locus_tag="ACHA6A_000445"
     CDS             29416..30441
                     /locus_tag="ACHA6A_000445"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8968680.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30456..31394
                     /gene="galU"
                     /locus_tag="ACHA6A_000446"
     CDS             30456..31394
                     /gene="galU"
                     /locus_tag="ACHA6A_000446"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8968681.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31519..31959
                     /locus_tag="ACHA6A_000447"
     CDS             31519..31959
                     /locus_tag="ACHA6A_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8968682.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31962..32564
                     /locus_tag="ACHA6A_000448"
     CDS             31962..32564
                     /locus_tag="ACHA6A_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8968683.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32623..33729)
                     /locus_tag="ACHA6A_000449"
     CDS             complement(32623..33729)
                     /locus_tag="ACHA6A_000449"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8968684.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33939..34940
                     /gene="ccpA"
                     /locus_tag="ACHA6A_000450"
     CDS             33939..34940
                     /gene="ccpA"
                     /locus_tag="ACHA6A_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8968685.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35277..37622)
                     /locus_tag="ACHA6A_000451"
     CDS             complement(35277..37622)
                     /locus_tag="ACHA6A_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8968686.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37901..38803
                     /locus_tag="ACHA6A_000452"
     CDS             37901..38803
                     /locus_tag="ACHA6A_000452"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8968687.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39016..40791
                     /locus_tag="ACHA6A_000453"
     CDS             39016..40791
                     /locus_tag="ACHA6A_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8968688.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40857..42443)
                     /locus_tag="ACHA6A_000454"
     CDS             complement(40857..42443)
                     /locus_tag="ACHA6A_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8968689.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42694..42918
                     /locus_tag="ACHA6A_000455"
     CDS             42694..42918
                     /locus_tag="ACHA6A_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8968690.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43059..44810
                     /locus_tag="ACHA6A_000456"
     CDS             43059..44810
                     /locus_tag="ACHA6A_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8968691.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44810..46594
                     /locus_tag="ACHA6A_000457"
     CDS             44810..46594
                     /locus_tag="ACHA6A_000457"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968692.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46920..47771
                     /gene="licT"
                     /locus_tag="ACHA6A_000458"
     CDS             46920..47771
                     /gene="licT"
                     /locus_tag="ACHA6A_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8968693.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47867..49747
                     /locus_tag="ACHA6A_000459"
     CDS             47867..49747
                     /locus_tag="ACHA6A_000459"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8968694.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49787..50689)
                     /locus_tag="ACHA6A_000460"
     CDS             complement(49787..50689)
                     /locus_tag="ACHA6A_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8968695.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            50920..52323
                     /locus_tag="ACHA6A_000461"
     CDS             50920..52323
                     /locus_tag="ACHA6A_000461"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8968696.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52323..53756
                     /locus_tag="ACHA6A_000462"
     CDS             52323..53756
                     /locus_tag="ACHA6A_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8968697.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            53901..54827
                     /gene="trxB"
                     /locus_tag="ACHA6A_000463"
     CDS             53901..54827
                     /gene="trxB"
                     /locus_tag="ACHA6A_000463"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8968698.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55041..56768
                     /locus_tag="ACHA6A_000464"
     CDS             55041..56768
                     /locus_tag="ACHA6A_000464"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8968699.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56783..57133
                     /locus_tag="ACHA6A_000465"
     CDS             56783..57133
                     /locus_tag="ACHA6A_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8968700.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        18550 a         9944 c        12341 g        16333 t
ORIGIN      
        1 gtatacttgt ttttcctttt atacatttta tggtattatc attttattag ttattataaa
       61 acaatcgtta gaaaggacgt cattatgcaa gtatacggaa ttgatgcggt gcttcgaatc
      121 gtgagtcatt tggcgtttat ctacttagcc ttctggtctt tacgttcgtt acgcattgaa
      181 acttttttca gaccaatgca taatacgcaa attagaatgg caatcgtact tttttcaatt
      241 tttttagggt acactgcaag taattttttc ttagaagtta tcgctttatg ccgaaatctt
      301 ttcgttagcc tgtaaatttt aaaaaaaggt actattttaa aagcaaggat gttataatag
      361 aaaagatttt gagtaagtgt atgctcattg gaattttttg gagggaacaa catggaagag
      421 atcatcgtaa gaggtggcaa tcaattaaat ggaacagtaa gaattgaagg agctaaaaat
      481 gctgttttgc ctattttagc tgctagtttg ttagcagaag aaggtattac gacattagac
      541 aacgttccga ttctttctga tgtcttcact atgaatcaag tgattcgtca cttaaacgta
      601 gatgtagatt ttgatgaaca aaagaatcaa gtaacaatcg acgcttcaag acaattagaa
      661 atcgaagcgc cttatgagta tgttagtcaa atgcgtgctt caatcgttgt gatgggtccg
      721 ctacttgcca gaaatggaca tgcaaaagta gctatgcctg gtggatgtgc gattggtaaa
      781 cgaccaatcg atttgcattt aaaaggattt caagctttag gagcaaaaat tattcaaaaa
      841 aacggctata tcgaagcaat tgcggatgaa ttgatcggta acacgattta tttagatttc
      901 cctagtgtag gtgctactca aaatatcatg atggctgccg taaaagcaaa aggaacaacc
      961 atcattgaaa acgtagctcg cgaaccagaa atcgttgatt tagcaaacat tttgaataaa
     1021 atgggcgctc aagtctatgg agctggtact gaaacaatgc gtattgaagg cgtggatcat
     1081 ttacatgcag tcaaccactc aatcgtacaa gatcgtatcg aagcaggtac ctttatggtt
     1141 gctgctgcaa tgacacaagg caatgtgctg attgcagatg ctatctctga acataaccgc
     1201 ccattgattt ctaaattgat cgaaatgggt gcagaaatca ttgaagaaga aggcggagtc
     1261 cgtgtcatcg gaccaaaaca tatcttacct acagatgtaa aaacgatgcc tcatcctggt
     1321 ttccctacag atatgcaagc tcaaatgaca gctatccaat tagttgcaga aggtacaagt
     1381 gtagtgactg aaacagtgtt tgaaaatcgt ttccaacatt tagaagagat gcgccgtatg
     1441 aacgctcatg tcaaaatcga cggaaatgta gcaatcatgg atggaaacca tgaattgcaa
     1501 ggtgcagaag tatatgcaac agacttgcgg gcagcagcag cattagtttt agctggcttg
     1561 aaagcaaatg gaatcacacg tgtgcgtaac ttgaattatc tagaccgtgg atattataat
     1621 ttccatatca aactacagca attaggtgca gacgttgaac gtgtagacat ggatcaaact
     1681 tctgctgaaa aaacagcaca aacgattgct taaggaagtg gctgaatgag taaccaacga
     1741 tatatcttat taactttgat aaagatttta atggtgatct tattgcttat ccttcttttt
     1801 gtggccggaa cgatgatcgg atatggtgtc atcggtggcg gcaatccatt taaagtattt
     1861 cagccaagtt tgtggattca tattcgagac ttttttcatt gataaataag aatcacagtg
     1921 gttctagtcg atatgaagtg agccatgaag cagaacatga taaaagaaaa cagaagggaa
     1981 cgttgacgtt agtcgcgatc ccttttttta gtaaaagaaa agaaggaatc taattgatga
     2041 agaagatatt gatttattta gttagaggat atcagcgctt tatttcgcct gcatttccac
     2101 ctagctgccg ttattaccca acctgttcca attatatgat aaaagcaatt caaacccatg
     2161 gcgcgctaaa agggacggta atggggactg cacggatctt acgctgtcat ccttttgtca
     2221 aaggcggtat cgattatgta ccattgacat tccgattaac gcgaaataag aaagataaaa
     2281 aagaaccaga ttataaaaag gttgtgaaaa atacgggtag cactgagcga taaagaaaca
     2341 atttggaaaa tagcttctta tattttgcaa aattgtgagt tattgccgaa gtgttggatt
     2401 ttgaacaccg tcaaaaaaaa ggttgtgaaa aatacggcta gcactgagcg ataaagaaaa
     2461 agaggaaatt tattgtcaaa gtaacaactc cgcaactact tatatggggg acaaaaaatg
     2521 atcgaagaac taaacaagaa agatttacca tggtccttgt tattagatgc agatcctgat
     2581 aaagaaaaag ttcaagtata tgtttccaga gggagtggct ggatatggaa agaaaaagaa
     2641 aaaacaatag gtgtactgat ctatgtggca agggaaaaag aatacgaaat cgtcaatgta
     2701 gcagtaaccc caacgtatca aggaaagggg attggcggaa aattacttga gacagctttt
     2761 cagaagcttt cacaactcgc ttccagtcaa actcagatca tcatacgaac cggcagtact
     2821 agcagtgcgg cgttacatct ctatcaaaaa atgggctatg tggagattgg tcgagtcaaa
     2881 gactatttta tccatcatta ctctgagccc atttttgaaa atggagatca attaagggat
     2941 caagtgattt tagcaattca cttacctaaa aatagcttta actaataaaa tttcaattca
     3001 agaaaatgct ttcaaatgct aaatagatac attacaatag ctttagcaaa caaaaaggag
     3061 aatattttta tgggaatatt agtttttgat tttggtggat cggctgtcaa atatggttgt
     3121 tgggatggca aagagattaa aggtaaggga caatttgcaa cgcctgaatc ttgggaagaa
     3181 atgaaagcgc aattatttca agtttataaa aaaatactcg tttcttttga agtcgaagga
     3241 attggaatca gctctcctgg agtagtgaat aatgagaaac aggtgattga gggaatcagt
     3301 gcgattcctt atattcatgg atttaatatt ttccgagatt tggaaactct gtttcaactt
     3361 ccagtaacga ttgaaaatga cgccaactgt gccggaatgg ctgaatttta tgaaggtgct
     3421 gcccgcgatt atcaagatgt tgcttttgtt gtggcaggaa ctgggattgg cggggcgttg
     3481 ttcacgaaag gacaaatcaa taaaggggca catctttacg gtggagaatt tgggttgatg
     3541 ttcttaaatg gcgataagac attcagtaaa cttggtacag cggtacagat ggcttggcgt
     3601 tattgtgaac gcaaaggtct cgataaaaat gcttataccg gaaaagatgt ctttgaatta
     3661 gcagaaactg gagatgcaat agcaaaagaa gaagttgaga gtttttatac gtatcttaca
     3721 aagggattat tcagtattca attttcgttt gatccagaag tgatcgtatt aggcggcggg
     3781 gtttctgcaa aagaagggct cattgatgag atcaaaagcc gaatgaagaa gctgacagaa
     3841 caattcgatc tgcatgattt cgaaccgcag attttattat gtgaatacag aaacgatgct
     3901 aatcttgtag gtgcagcagc aaactatctc gctgtacgtc aaggggagaa ttaagagctt
     3961 tagagaaggt gcagcttgca taaaagaagt gaatgaaatg aataggagat ttttacctaa
     4021 ggaatgtttt atttgctgca gtatgataaa atatctgaga ggtgaaaatg atgtcaaaaa
     4081 aaaataaaga tattgaagtg cgtatagaag aagtgaaaaa agatatcaaa ggtaagaatt
     4141 atgaagtcac tcagttattt atagggaaaa aaatgattgg tgagatctta gcctatggac
     4201 caaaagaata tgaaattttc tttggtgaag aagattttgg aaaagaaaaa agtcttgaaa
     4261 atgcgataga aacagtgatt cgacactgga atctgcacga ataaaaaaat atcaaaaaaa
     4321 ctttcaataa agggttgcaa aaatctggaa aattaggtat tataactaag tcaggttgat
     4381 acaaaccttt cggaggagta gcgaagtggc taaacgcgac ggactgtaaa tccgttcctt
     4441 agggttcagt ggttcgaatc cactctcctc catttcattg gggtatagcc aagcggtaag
     4501 gcaagggact ttgactccct catgcgttgg ttcgaatcca gctaccccag tataccacaa
     4561 tcgtaagctc tcgttagttc gagagctctt ttttttgctt tgctaccttg ttccttgaag
     4621 attctgctga tttaagatag agttaagtta actacaagaa ttggagggat ccacatgaaa
     4681 tggaaagaaa aagtagacga agcagctgaa aagttatatg atttggtcaa atcagaaaaa
     4741 tacaatattg aagtgaatat tccgaaaaaa ggcggaaaag cggtccgggt aaaatcaaaa
     4801 cgcccaacga atcatacaaa aaaatggatg gctaaaaacc gctaataata acaaaagatc
     4861 cataagtcgg cattttcatc gattttaggg tcttttgtta ttttctaggt tggacatatc
     4921 taaaaaattg tttctgacaa taggagtagg aagaacgatg tgtgtctaaa tcctctgttg
     4981 atggatagaa acctttatct ttttcagttg tgaatcaggt atataccagt tataatagaa
     5041 gaaacgaaag gacgggtgta cgttatgtcg aatcaattac ctgtatatat acagattcat
     5101 gaccaattaa aaaatgaaat cgaacaaggt atatggaaag tcggagaccg tcttccttca
     5161 gaaagagaat tagcagtgaa attcaatgtc agccgtatga cgttgagaca agcaattcaa
     5221 aacttggcag atgaaggaat cttagaaaga aagatcggtt cgggtacata tgttgcacga
     5281 gaaaaagtac aagaaaaaat gtctggtgca acaagtttta cagaaattat ggaatcacaa
     5341 aatcgagtac cgtctagtcg gacaatctct tatttcttga cttcgccaag ctcaagtgaa
     5401 atggaaaaac taaacttgag taaagatgac acgatcttgc gaatggaacg tattcgtttc
     5461 gcagatgatg ttcctatttg ttttgaagtt gccagtgtgc cacaaaagct gatcgatggc
     5521 tacagcaaag cagagattac tgaatcgttg tatcgcacgc tagaagaaaa aggcgggaaa
     5581 aagatcggtc cagcgaatca aacgattact gctgtgttgg cttctgagaa aattgctgac
     5641 tatttagagg taaaacgagg agatgcagtt cttcgtttgc gccagatttc ttattttgaa
     5701 gacggtacgc cgtttgagta tgtgcgtacg caatacgtag gaaatcgttt tgagttttat
     5761 ttggaaaaat aaaaaacagg tgatccctga ttccgtatta gaaataaaag agaatcagaa
     5821 tcacctgttt ttatatatga tcagttacca gttgctcgtg ataagaaaca ggaccctctt
     5881 caaattggac ttgtccgttc agcagctcaa tgatggattc tttaaagtgg tcagcacttt
     5941 tttcatctac agcacagatg acgctaactt gatcagtata ttggatttct tttataaaaa
     6001 aatgttggag ttcaagataa ttctgtaatt ttcctagttg cggatacgtg atcatcacag
     6061 agatctcctg ttgcaaggta ccttctacga ttccaatgtc agacaatgct tgagagaccg
     6121 catgagtgta tgctcgtatt aatccgcctg tgccgagttt cgttcctcca aaatatcgag
     6181 tgactactgc agctacattg atcagttgat tttttttgag gacttccaac atcggtatgc
     6241 ccgccgttcc acttggttct ccatcatcgc tgcttcgctg gatttcattt ttttctccaa
     6301 tgatgaaggc gctgcagtta tggttcgctt tccaatgttc tttttttatt tcttgaatga
     6361 aactttttgc ttcgtcctct gattctattc gtctaagaga acagataaag cgtgattttt
     6421 tgatctcgat ctcactttgt ccattttctt ttattgtacg atagcgctcg atcatcttgt
     6481 cacctctttt ttcattttct ttattcagta taaataaagt tctaggttgt ttcaacactg
     6541 taacggttgc gcaattgctg tagtatgcga taataaagac aaaggcaggt ggatctgggg
     6601 atgcaggaac gaatgaaaaa gtacgatgca attacgcatt atttgaagaa caatggcggc
     6661 tctcaagtaa cgttgacctt tacccaattt gacgaactgc tgtttcccag taacggactt
     6721 cccaaaacag caagggaaag tacagattgg tgggcaaatg attataaaca ccctgaaaaa
     6781 ggtgcctatg gctggataaa tgcaggctat gaagtagttg tcatcaattt ggacaaagaa
     6841 tatgtcgtgt tcaataaatt agtgaaatct agttggctat ttgattaaat aaaccgcgtt
     6901 tagaaaacgt tgacaaattt ctttcttttt atataataga gtaacagacg cgaagaaaac
     6961 gtttgcgtag tttgttttaa aaaggagaga aaagtatggc taaaaaaaca atcatgcttg
     7021 tatgttcggc tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa gctgctgaag
     7081 caaaaggaat cgactctgat attttcgctg tgtccgcttc tgacgcagat aacaatcttg
     7141 caaataaaga cgtggatgtg ttgttattag gcccacaagt tcgctttatg aaagctgatt
     7201 ttgagaaacg tctggaacca aaaggcattc ctttagatgt catcaatatg gctgattatg
     7261 gtatgatgaa tggcgaaaaa gtgttagatc aagcaatcac gctcataggt gaataaatag
     7321 aacagtataa ctatcgaaca agcgatccaa aaaatagatc gcttgttttt tcgtatattt
     7381 ttttagggcg aataatatga atcatttttg gggtagaagg cttctgacga gagatattaa
     7441 agcaatagac tgtgaggagg taaatttacc aaattatgag aagatcgcag cagtggaacg
     7501 tttgagcaac tggattcttt gtcatcgctg tggaatgaga acgtccgtca ttgatggaca
     7561 gcttgcagat tacggttatt tttgtacaca ttgtctatat ttagggcgat gcgacactca
     7621 acaagattta tatttgtttg atcagcctga agcaaaacct cgagaagtcg tctttttttg
     7681 gacaggacag cttactgaaa aacaaacgga aatctcaaag agaatacttt gccattcaga
     7741 aaaaagacat catcttattt gggctgtaac aggtgcaggg aaaacagaga tgctttatcc
     7801 tattttagtg gaaactttaa aagctggagg aagagtagcg atttgtacac cgcgagtcga
     7861 tgtttgtaat gaattgtttt tacgctatcg ttatgttttt ccagaagaaa aaatcacgtt
     7921 gctccatggg aatacagcaa cggcttatac ctttacttct tttgttatct gtacgattca
     7981 tcagctatac cgtttttatc ggtcctttga tttaatagtg atcgatgaga tcgatgcgtt
     8041 tccttataac ggggatgcag gattagctca tgctgtgcaa acagcgatta aaccagacgg
     8101 acgattcatc tacttaagtg ccacacctga taaaaagtta ctaaaggaga tcaagcagac
     8161 gtttgacatc cacaaacttg ctttacgctt tcatcggcgt ttgcttcctg aacccgtctt
     8221 ggttttcatg aataattggc gacaaaaatg caagaacaag aagaagataa aatctttggt
     8281 gcggatcatt catcaattaa ttaaagaaaa ccatatcctt ttattttgtc caagtatcac
     8341 gatgatgcat cgtttgaaag ccacacttca agaggctctt ccgagatatg agatcacaga
     8401 tgtttcttca agagacaatc agcgggcgga aaaagtccag aaaatgcgag aagcaaaata
     8461 tgacatactg ttaaccacga tgatccttga acgtggagtc acatttgaaa gaatatccgt
     8521 tatcgtttta ggagcggatc atccggtttt tactaaatct tctttggtac aaatcgcagg
     8581 aagagccgat cgtaaagggc cttatacgaa cagtcaagta tattttttct atgaagaaaa
     8641 aactgcagct attcgaaaag cttgtcagga aatcaaagaa atgaatgaag aagggaagaa
     8701 gatactgtga ggtgcagttg ctgcagtaaa gatatcgtcc aaaatttaac attcagcgag
     8761 attattggtg tttcgaaata cagattacct cagacttgtt cagtttgtcg cgcacaattt
     8821 catttattgg aaacaagagg ctgttctggc tgtcaaaaac caatccattt caatctagag
     8881 agtacgaaaa agaaagtaaa ttctatcccc gtttactgca gtgactgtca agaatggaag
     8941 aagaaatatc cgacctatga ttttcagcac cgtgcctttt ttgtgtacga tgaaaatatg
     9001 aaggcctggc tcaatcaata caagtttatg ggagatatcc gactagcagg cacctttgca
     9061 tataaatgga cgcagataaa aaaagaatac ccgacataca tctattgtcc aataccttta
     9121 tctgaaaaaa ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt gaaagcagca
     9181 agaattcctt ttcagatgct actcaaaaga gagaagcatc tgctggctca agcaaaaaac
     9241 acgcgaaagg aaagattgga gatgcctcaa ccttttgaac ttgcagtcac cttttctaag
     9301 atacaaaata aaaaaatatt gattattgat gatgtctata caacaggaca gactcttttc
     9361 cacgcagcag attgtttgca gatcgcacag ccgaaaatca tccgaactat ttctttggcg
     9421 agatgaatta gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat
     9481 ataatagaat taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa
     9541 aggggtaatt tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc
     9601 gattcgcgac tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc
     9661 cgatgcaacg gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt
     9721 aacgatccca ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc
     9781 aagtatcgat ttggttgttg acaaattgga acgccaaatc cgcaaattca aaacaaaaat
     9841 taatcgtaaa tcccgtgaaa cgggcatgaa tactgcaaat gctgctgctt tattcaatga
     9901 agaggacgaa aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt
     9961 gaaaccaatg gatagtgaag aagcagtctt gcaaatgaac atgctgggac ataacttctt
    10021 tatttttgaa gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa
    10081 atacggactg attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc
    10141 agctactgac agctgccgca agtttttttg agtttcctcc tcttttagat gaaatatgaa
    10201 taaattatga agttcttact ttaatacgct taagatattt caattatcta taactaatga
    10261 taaaatgaaa agtaaagtct acatagtctg gataatacac agaaggaaaa ggagattaca
    10321 caacgcatgg ccaatttgat tcgttcactt atcgaaaacg ataagaaaga actaaaacgt
    10381 ttagatagca ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc attaactgat
    10441 gaacaattac aggcaaaaac tgctgaattc aagggcagat accaaaaagg agaaacatta
    10501 gatcaattac ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg tgtattaggt
    10561 ttatatccat accacgtaca attgatgggt ggaatcgtgc ttcacgatgg aaatatccct
    10621 gaaatgcgta ctggtgaagg taaaaccttg accgcaacga tgccagtata tctaaatgct
    10681 ctgactggcg aaggggtaca cgtagttacc gtcaatgaat atttagcaac ccgtgactcg
    10741 acggaaatgg gcgaacttta taatttctta ggattaacag tggggttgaa tatcaactca
    10801 aaatcttcgg atgaaaaacg cgaggcttat aattgtgata tcacttatag tacgaataat
    10861 gaattaggat ttgactactt gagagacaat atggtcgttt accgccatca aatggtacaa
    10921 cgtccgctga attatgccat tgtcgatgaa gttgactcaa tcttgattga tgaagcgaga
    10981 acgccgctga tcatttcagg gcaagcagaa aaatccacag ctttatatac aagagcagac
    11041 aacttcgtga aacgtttgaa agaagaagaa gattataaaa tcgatgtaca atcaaaaaca
    11101 atcagtttaa ccgaagcagg gatcgaaaaa gcagaagaaa cattcggttt ggataactta
    11161 tacgacattg aaaatactgc attgacccat cacttggatc aagcattgag agccaactat
    11221 atcatgcttt tagatatcga ttatgttgtc caagacggaa aagtactgat tgttgaccag
    11281 ttcactggtc ggatcatgga tggacgccgt tattcagatg gacttcacca agcaatcgaa
    11341 gctaaagaag gcgtagagat cgaagacgaa acgaaaacca tggcgacgat cactttccag
    11401 aactatttcc gtatgtacaa gaaacttgcg ggaatgactg gtacagctaa aacagaagaa
    11461 gaagaattca gagaaattta taacatccaa gtataccaga ttccgacaaa tcgtccggtt
    11521 atccgtgatg atcgtccaga tttgctttac ccaacattac aaagcaagtt ccacgctgtc
    11581 gtgcaagata tcaaagatcg ctaccgcaaa ggtcaacctg tgttagtcgg aactgttgcg
    11641 gttgaaacat ctgagttatt atcagatatg ctgaatcaag aacgtattcc tcatgaagtg
    11701 ctgaatgcga aaaatcactt taaagaagca gagatcatca tgaatgctgg tcaaaaaggt
    11761 gctgtgacga ttgctaccaa catggctggt cggggaacag atatcaagtt aggacttggg
    11821 gtaagagaac ttggtggtct agcagttatc gggacagaac gacatgaatc tcgtcgtatc
    11881 gataaccagc ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc acagttctac
    11941 ttgtcattgg aagatgattt gatgaaacga ttcggttcag aacgaatcaa atctttctta
    12001 gatcggatga agatcggtga ggaagatgct gttatccaaa gcaagatgct gacgaaacaa
    12061 gtagaatctg cacaaaaacg tgtagaagga aataactatg acacacgtaa aaacgtctta
    12121 caatacgatg atgtgatgcg tgaacaacgt gaagtaatct ataaacagcg acaagaagta
    12181 atcatggaag aaaaaagctt atccaagcca ctaatgaaca tggtcaaacg gacaatcagt
    12241 cgtgtagtag atgcgcatac acaattagag aaagaaaatt ggaatttgga aagtatcatc
    12301 gattttgccg gcagtactct tgtccatgaa gatagtatta ctcttgcaga tatcgaagga
    12361 aaaacagctc aggagatcaa agagtatcta aacgaacgag cacaagaagt atttaatacc
    12421 aaagcggctc aactaaatgg tccagagcaa ctgttggaat ttgaaaaagt cgtgatcttg
    12481 cgtgttgtgg actctaaatg gacagaccat attgatgcaa tggatcaatt acgtcagtca
    12541 atcggcttac gtgcttatgg acagaacaat ccattagtcg aatatcaaac agaaggctat
    12601 aagatgtttg aagatatggt aggcgccatc gagtatgaag tcactcgaat ctttatgaaa
    12661 gctgagatcc gtcaaaacgt acagcgtgaa caggtttctc aaggaacagc ttctcactca
    12721 gaagacggag atgccaaaga aaatagtaat actagcgcga aaaaacagcc aatccatgtg
    12781 aacaaggtcg gaagaaatga tccttgtcct tgtggaagcg ggaaaaaata taagaactgt
    12841 catggaaaaa atgcaaacta atttctgaca gcaggattgg tgagacaaat tcttttttta
    12901 agaattcgtc tcaccttctt tctgttattt gctgattctg ttagactaga tataatgaaa
    12961 aaagtatagt gaaagaagga aaaaacatgg aaatatccga aattcgaaat ctcctgacag
    13021 aaatgcagga aaaggtcaca agcttcagga ggtctctttg acttagacca gctggaagaa
    13081 gacattgcgg aagcagaagc aaaaatggct gaacctggtt tttgggatga tagcgaagct
    13141 gctcaaaaag tgatcagtga aaacaatgca aataaagaaa cttatgatca attccattct
    13201 cttgctgaag aactggaaga aatcgaattg atggtggaga tgttgcagga agaacctgat
    13261 gcagccatgc aagaagaagc agaaacacgt atcaatgcgc tagatgaaaa aatgaagaca
    13321 tatgaattat ctatgttgtt agatggtcct tatgatcgaa acaacgccat cgttgaattg
    13381 catccagggg ctggcggaac ggagtcccaa gattggggca gtatgctttt acgtatgtat
    13441 acacgctggg cagagcagca tgggtatcaa gtagagacac tagattatca agcaggtgat
    13501 gaagcaggaa tcaagagtgt gaccctactg atcaaaggac acaacgcatt tggttatctg
    13561 aaatcagaaa aaggtgttca tcgccttgta cgtatctcgc cttttgactc agcgaaacga
    13621 cgtcatactt ctttttgttc ggtagatgtg atgccagagc ttgatgaaac gatcgatata
    13681 gaaatcaatc cagatgattt gaaaatcgat acgtaccgag caagtggtgc aggtggtcag
    13741 cacatcaata aaacagaatc tgctgtacgg atcactcact tgcctacagg aacagtagta
    13801 gctagtcagg cacaacgttc acaattgaaa aaccgtgagc aagcgatggg gatgttaaaa
    13861 gctaagcttt atcaattaga agtggaaaag aaagaacaag aagcagctgc acttcgagga
    13921 gaacaaaaag aaatcggctg gggttcacag atccgttctt atgttttcca tccatattct
    13981 atggtaaaag accatcggac aaacgtagag acaggaaatg tccaagcagt tatggacggt
    14041 gaccttgatt tatttattga cggctattta aaaatgcatc tacaataaga acccattgtt
    14101 aaattgaaat aaaattgtaa gattttgcaa gcatgttgaa acattgccat gctataatag
    14161 ctaaagactg aaaaagatat ggagagaacg ccatgattga aatgaaggat gtaatgaaga
    14221 agtactcaaa tggtacgacc gctatccgca atctctcaat tgtcatcgaa caaggagagt
    14281 ttgtctatgt ggttggtccg tctggagccg gtaaatctac attcatcaaa ttgatgtatc
    14341 gagaagaaaa agcttcaaaa ggaactttga acgtcgcagg tcatgatcta atagcaatca
    14401 aaaaccgcga agttccttat ctacgcagag aaatcggcgt ggtgttccaa gactataaat
    14461 tattaccaag aaaaacagta tatgaaaatg ttgcgtacgc tatgcaagtt atcggcagaa
    14521 aacctcggga tatcaaaaaa cgagtaatgg aagtgctgga ccttgttggt ttgaaacaca
    14581 aagttcgagt ctttcctagc gaattatccg gtggggaaca gcaacgtgtc tctattgctc
    14641 gggcaatagt gaatacgcct aaagtactta tcgcggatga accgacagga aatctggatc
    14701 cagaaaactc atgggaaatc atgaaattat tagatcggat caatgcccaa ggaactactg
    14761 tcgtgatggc tacacataac agtacgatcg taaatacgat tcggcaccga gtaattgcta
    14821 tcgaaaacgg ccgcattatt cgagatcaag cggaagggga atatggatac gatgattaga
    14881 acctttttcc gccatttatt agaaagtatc aagagtttga aacgtaacgg ctggatgaca
    14941 gttgcttcag tcagtgcggt aacgatcaca ttgactttag tcggtgtatt catggcagtt
    15001 atcatgaacg caactaaatt agctcaagat atagaaggaa acgttgatgt ctctgtattc
    15061 gtagatatcg gtacaaaaga cgaagagatg aaaaagctag aaaaagaatt gaatgactta
    15121 gatcatgtta aaaaagttga attttcaagc aaagaacagg aattgaaaaa aatccaaaac
    15181 gaaatgggtg attcctggaa attgtttgaa ggtgacaata acccgttata tgatgtgtat
    15241 gtagtaagtg ctactgatcc ttcttataca aaacaagtag cagaagaagc agcagatttg
    15301 aagaatgttt ctcgttctga ttacggtgga gcttcctcag atcggatttt ccaaatcgct
    15361 ggtgcagttc ggacatgggg attagttgca gcggcattgc tgctgttcgt agcgatgttt
    15421 ctcatttcga acacgattcg gatcacgatt ttgtctagac agcgtgaaat tcaaatcatg
    15481 cgtttagtcg gtgcaaaaaa tggatatatt cgctggccgt ttttccttga aggtggctgg
    15541 atcggtctga tcggtgcgat tcttccgatt ttgatcatca catttggtta tgcatggttt
    15601 ttccaattga tcaatccatc attattacgt tcccattatt cattgattca tccacaaaat
    15661 atcgtttgga aaatcaatct tttaatggtc ggcattggtg tgatcattgg ttcacttggc
    15721 tcgatcattt ctatgagacg tttcttgaaa ttctaatacc acaatataaa taaaatgaga
    15781 aagttggaca gtttcgttcc agctttcttt ttttattggc taggagaata gaaggaagcg
    15841 gcttttttcc ctttacttta aaaaacaact tgggtattct taagttggta gaagaagagg
    15901 tgaagccgtg aaaattatcg ttgacaattt ttttacgatc cttttgatta tgaatatttt
    15961 gctttccttt atcatcgttt ttcgagaaag aaaggaaact gctcaaacat gggcatgggt
    16021 gctcgttttg atgttcattc cagttgtagg atttatttta tatatttttt taggaagagg
    16081 gatttccaaa gagaaaatct ttgatttgaa gatccaaggg aaaatcggta agaacctgga
    16141 aattgaagaa gacaaacagg cattgatgcg agggctttat cctcatccgc ctactggaca
    16201 agtagatgtg aaacaattga tctatatgct cacggtattt gaaagtacgc tctatacgac
    16261 taaaaatgaa atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc tgattgaaga
    16321 tatcaagcag gcagaagacc atatccactt ccagtattat atttatcgca gtgatgcatt
    16381 aggtgaagaa gttcgagatg cattgacaga ggcggcaaga cgaggggtaa aagtcagagt
    16441 ccttcttgat gcatggggtt ccacacaagt ttcttccagc ttttttcaga atttgaaaaa
    16501 agcaggcgga gaagtcgcat ttttctttcc tctatttgtt ccgtatatta atcctcggat
    16561 caattatcgc aatcatcgta aaatcgtagt tattgatggg aaaatcggat atacaggtgg
    16621 gttcaatgtt ggaaatgaat atctaggatt ggtaaagaaa tttggttatt ggcgagacaa
    16681 tcatttgcgt atttacggag aagcggtgta taccttgcaa aaccgttttt tgatggactg
    16741 gaattctcag catacaaaag aagtaggcta ttctcccaaa ttttttccgt ccattcactc
    16801 tactggtgat atagctgttc aaatcgtgac aagcggacca gataccgaac atgaacaaat
    16861 caaaatgact tatttgaaaa tgatttcgtt agccaaacgg gagatattga tccaaacgcc
    16921 ttattacata ccggatggat cgattcatga agcactgaag ttggctcttc tttcaggcgt
    16981 aaaagttcat atccaaatac ctaacaaacc agatcatctt ctcgtttact gggcaacgta
    17041 ttctttcgct gcagagctaa tcgagtatgg agcaaggatc gaaacatatg agaatggatt
    17101 tattcatgca aagacgatga ttatcgatgg tggcattgtt tcagttggct cagcaaatat
    17161 cgatgttcgt tcatttcgct tggactttga ggtcaataca ctggtttatg atgaacgaat
    17221 ggcttcacgt gtaagaaaag ctttttttga agactcgaaa atttctacgc atctcacaaa
    17281 agaaatgtat gaaaaccgag gaataatcat caagatgaaa gaaggattag caagattgat
    17341 ttcgccgctc ctttaaaaca taaaaaggac atgagaacca tcaacagatg gagatctcgt
    17401 gtccttttct aacggggcca tttctgagag tacgtcagaa atggagaaag cgtctcgcat
    17461 gagcgaaatt aactttgggg tatctcttgt tttttgggga aagcaagaaa taagctaata
    17521 tatgctatta aaagagacac atttcaaagc cagtatagca tagaaacttt taatgagata
    17581 gtttttttta caattatgag aatttatttt gttatttaat gattttgatt cgttttcatc
    17641 aagtaatgaa aatgataaaa taaacatact ttaggattaa aggagatttc taatgaaaaa
    17701 acttaaatta ttcttaccaa taataggatt agctattctt ttatcaggct gttcaaagtg
    17761 gattgatcga ggagaatcca ttacggctgt tggctcttct gctttacagc ctttagtaga
    17821 aacagttgct gaaacttacc agacagaaaa tcctggaaaa ttcatcaatg tacaaggggg
    17881 cggaagcgga actggtttgt ctcaagtaca gtcaggagca gtcgacatcg gaaactctga
    17941 tttgtttgcg gaagaaaaat cggggatcga tgcctcagca ttagtcgatc atcgagtagc
    18001 agttgttggg atcactccta ttgtgaacaa ggatattggc gtaaaagaca tcagtatgga
    18061 aaacctgaaa aaactattta caggcgagat caaaaactgg agtgaagtcg gtggaaaaga
    18121 tcagccagta gttatattga accgcgcttc aggaagcgga acccgtagca cgtttgaaaa
    18181 atgggtattg gacggagaaa cagctgttca ggcacaagaa caagattcaa gcggaatggt
    18241 gcgccagatc gttgccgaca cacccggtgc aatcagctat gttgcttttt cttatgtttc
    18301 cgatgatgta gatacgttga atgttaacgg tgtagaaccg acagatgaaa atgtgacaac
    18361 gaacaaatgg acgatttggg catatgagca tatgtacaca agagatcatc caaaaaaact
    18421 gacaaaagaa tttttagact atatcttatc tgatgaagta caagataaca tcgttggaga
    18481 actaggttat atccctgttt ctcatatgaa agtcgaacgt gattggcaag gaaatatcgt
    18541 taaagagtaa tctttacaca aaatttacaa acttagctaa acttttacat gttattaaaa
    18601 ccaaattgat gttcaatttg tacactgagt gagtatgtta aacctgatta agggggagtc
    18661 atctgtggaa gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa aaatggaaca
    18721 gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc gtgttggttg tattatctat
    18781 tttttatttt gtagctagta aaggattggc cacctttttt actgataaga tcaatgtatt
    18841 tgattttctg tttggtacag attggaatcc aagccaaatc ggagcaaatg ggaaaccaat
    18901 ggtaggggca ttgcctatga tcacaggttc gtttattgtg acctttttat ctgctatcgt
    18961 tgccacgcca tttgccattg gtgcagcagt attcatgacg gagatctctc ctaaaaacgg
    19021 aacgaagatc ttacagccgg tcatcgagct gcttgtaggt attccttctg tcgtttatgg
    19081 ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt atttttggcg ggactgggtt
    19141 tggtatctta gctggtacat ttgtgttgtt cgttatgatc ttgccgacta tcacaacgat
    19201 gactgttgat gcattaaagg cagtgccacg gcactatcgg gaagcttcat tagcgcttgg
    19261 tgccacaaga tggcaaacaa tctacaaagt cgtgctacga gcagccgtgc ccgggatttt
    19321 aacagcagtc gtttttggta tggctcgtgc gtttggtgaa gccttagcta tccaaatggt
    19381 catcggtaat gcagcattga tgccaacagg tcttactact cctgcttcta cattgacgtc
    19441 catcttaaca atgggaatcg gaaatacaat catgggaaca gttgaaaata atgtgttatg
    19501 gtcattagcg ctgatcttgc tattgatgtc attattcttt aatatcgtga tccgtatgat
    19561 tgggaagaaa ggagagctga aataatgaac gcaaaacgct cagataaaat tgctacaggt
    19621 gttttatata tagtttcggg gatcatcgta ttgattttag cagctttact actttatatc
    19681 ttagttcggg gaataccgca catttcatgg tccttcttga ccgaaccttc caaagcctac
    19741 caagaaggcg gtggaatcgg gatccagcta tttaactcga tttatttgct tttgatcacg
    19801 atgatcatca gttttcctat ttctttaggt gcgggtatct atctttcaga atatgctgga
    19861 aaaaactggc taacagatgt gattcgcacg tcgattgaaa tcttgagctc gttgccttct
    19921 gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc aattccagta tggtttctcg
    19981 attatttctg gtgcattggc tttgactgta ttcaacttgc ctcttttgac tagaacagtt
    20041 gaggagtctt tacaagcggt ccattacaca caacgagaag caggattggc cttaggtttg
    20101 tcacgctggg aaacagtgat aaaagtggtg gttccagaag cgacaccagg gattttgact
    20161 ggtgtgatcc taagttctgg aagaattttt ggtgaagcag ctgcattgat ctatacggct
    20221 ggacaaagtg cacctgcgtt agattttacg aactggaatc cattaagtgt ttcaagcccg
    20281 atcagcattt tccgtcaagc tgaaacatta gctgtccata tttggaagat caatacagaa
    20341 ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt gattattgct
    20401 gttttactgt tcaacttcgg cgctcgtgcg attggcaaac gattataccg aaaaatgact
    20461 tcagcctaag gagaagaagc aaaatgaaaa aatataactg gaatgaaaca aatgtcatca
    20521 ccatgccaaa agagaaagaa gtagcactat atacggatga tctacatgtc tggtatggcg
    20581 acaatgaagc aatcaaagga gtggatctgg aatttgaaaa aaataagatc actgcactga
    20641 ttggtccttc cggctgcgga aaatctacgt atcttcgttc gttgaatcga atgaatgatg
    20701 ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca aggcgtagat attaatgcgc
    20761 ctcaagtgga tgtttatgag atgcgcaaac ggattggcat ggtttttcaa cgtcctaatc
    20821 cgttcagcaa gtcgatttat gacaatatta catttgcttt gaaacagcac ggcgaaaaaa
    20881 acaaaaagaa attagatgaa atcgttgaaa caagcttgaa acaagctgcc ttgtgggacc
    20941 aagtaaaaga cagcttggac aaaagtgcct tggctttatc tggcgggcaa cagcaacgtt
    21001 tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt gctgctagac gagccggcaa
    21061 gtgctttgga ccctatttcg acaggaacgg ttgaagaaac acttgtaaac ttgaaagaag
    21121 actatacgat catcatcgtg acgcataaca tgcaacaagc agctcgtatc agcgactata
    21181 ctgccttttt ctatttagga aaagtaatgg aatatgacaa aactaacaag atatttacca
    21241 gaccaaaaat tcaggcaacc gaagattatg tgtccggtca ttttggttaa gacggaggcc
    21301 tcaacatgac aaaagagatt atttcatcta aagatcttca tctgtattat ggaaagaaag
    21361 aagcactaaa agggatcgat ctaagcttca atcaagggga aatcactgcg atgatcggtc
    21421 cttctggttg cggaaaatcc acttatctgc gttctttgaa ccggatgaat gacctgattc
    21481 cgaatgtaac gatcaccgga agtgttttat ataaagacaa agacatctat ggtccaaaaa
    21541 cagatgttgt tgaactaaga aaagaaatcg gcatggtatt ccagcagcca aacccatttc
    21601 ctttttctat ctatgaaaat gtcatctatg gattgaaatt aaaaggggaa aaagataaaa
    21661 aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc gtctgtttgg gatgatgtga
    21721 aagacaaatt gcataaaagt gctttatctt tatccggtgg gcagcagcag cgtgtatgta
    21781 tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt ggatgaacca acaagtgcgc
    21841 ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt agaattaaga gagcggtata
    21901 cgatgatcat cgtgacccat aatatgtctc aagcttcacg tatttcagat aagacggcat
    21961 tctttttaga tggtcacttg atcgaataca atgatacgaa gaagatattc atgaatccag
    22021 aaaaacaaga gacagaagat tatatttcag gtcgatttgg ataaggagga aacggaatgc
    22081 tacgtacaca atttgaagaa gaattgctga atctccataa tcagttctac gaaatgggga
    22141 tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata catcaaacac gataaaatct
    22201 tagcccaaga agttatcgaa aacgatataa atatcaataa catggaaaca cgcttggaga
    22261 agaaaagttt tgaaatgatc gcattgcaac agccagttac aactgatttg cgtatgatca
    22321 ttacggtcat gaaagctagc tcggatttag aacgtatggc cgatcatgcg gtgtctgttg
    22381 ctaaatccac gattcgcgta aaagggcaga cgagaatacc ggaaatcgaa aaagagatct
    22441 ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt cctagttgct tatgtcaaaa
    22501 cagacgaaaa agacgcacgt acgatcgcca atatggatca aagagtcaat gactatttca
    22561 atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa tcctgagaca gtcatcagtg
    22621 gaacagacta cttgaatgtt gccagttatc tagaacggat cggagactat gtgacaaaca
    22681 tctgtgaatg gatcgtctac ttggctacag ggaaaatcac tgagttaaat acgaaccaca
    22741 atgagatttt ctaaactgaa gctgtgatta tgccgtttgt ttaggaaaag aggttgtgaa
    22801 atcagcggtt gtgaaaaagc tgttctgcat caagtaataa gaacagtcaa acaaaaaata
    22861 gctcctcata tttttcgttt ggctggactt attaccgcag atgcaagctt ttgaacaccg
    22921 tttattcggg aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc atttcataga
    22981 aaggataaag aatgcaaaaa aaaattagca tcatcactat attttttatt tgtgtgctta
    23041 cttcatgggt tattcagcat caagccgctt tatatgaagt acctgtcatc gagatagaaa
    23101 aagtacgaat aaaagaggat catcaattga ttacaggaaa actgatgaac acggatcaaa
    23161 aaggagaatc gatttattta tcggttcctt atcaaaaaaa tcaaatagaa acagcaaata
    23221 taaaagcgag acaaatgtat ctagtccaaa tgcatggaga tgaatggcag ctagtagcaa
    23281 aaaaaaatga tggctggctt ttcttcttca caagtatctt cattggcgcc atgttgctta
    23341 ttggtggaaa acagggtcta tttgctttag gaagtctcat catcaatatc gttttacttt
    23401 tacttttgtt gctcatcttt actaaaacag aagggatttc tctattaaat atatctatcc
    23461 tgttcgtcgt tatagggatg attattagca tattggcttt agatggatgg aatagaagca
    23521 gtataatcaa aatttcagct gcagttacga gtgttttttt agcatttttt atttgtcttt
    23581 tgacaatgaa tcattggaaa gaccaaggac tgcgctatga ggaactgaat tatctgacac
    23641 gaccgtatcg ttctgttttt ctttctagcg tactgattgg aacagctgga ggaacactcg
    23701 ataccgttat cacagtcatt gcgacattag aagaattgac taagcgacaa ccagaaataa
    23761 cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg agatgtgatt ggctcgatga
    23821 ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt ttatatttag
    23881 gaaatcaatg gtcgttcaaa gaaacgatag aacagcattt atcattggaa tttttacgcg
    23941 tcattgctgg cagcttagga atagtattat cgatccctat ctcggtcctc ttttttctga
    24001 ctgtaaggag gctgaaacga tgaatgtact tattatactt gttgggattt tagcaatcag
    24061 cgtgctactt attggcggta cgcaaggtat gtatattctt ttaggcttat tcatcaattt
    24121 agggatcttt ttcctgttgt tattcggtta tcatcagaag tggccgatac tggcattgtc
    24181 ggtcatagga tttttattga ttgctgtggt tatcttattt tttatcaacg gttataatct
    24241 aaaaatgcgt gcagcttttg tgagtatact gatcttttta ttttgtttcc tactattgct
    24301 tcctatcact gattttttag caatccaagg ttttacatcg gtagaactag aagaattatc
    24361 tggcttagat aagactttag cgattgattt tagattactt acgagatcgt tgcttctgat
    24421 tagtcttagt ggtgctgttt tagatgcgag tgtagcaatt tctagtggga cattcgaagt
    24481 atatcaggca aatccgcaat tatcttttta ccaattgcgc cattctagtt tcgctattgc
    24541 taaaaaaata ttggcgtcaa cagtaatgac acttcttttt gcattcatgg gaagttcttt
    24601 agcgttgatc ctctggttta ttgatttaga cattcctttt cagcaagtga tcaatgaaaa
    24661 atcatttgtt ctagaatata cgatggctat tcttaccaca ttgtccgcac tacttgttct
    24721 tcctttaacc tgcgtgacta cagcatatct ctttacaaaa aagaaagaac aactcaagat
    24781 ggagtaaaag ttacaactta agtcctatga catttttact gaatcagtgc ataatatgga
    24841 tgtaaggaaa agcttgcaaa caattttgct taaggaggca atttccatga aagaaagaga
    24901 acgtatttta gatttagtga aaaaaggaat cctttcgact gaagaaggct tagatttatt
    24961 agaaagcatg gcaactgaaa aagacgaaaa gcaaatcaaa aaagaagcag acaaggtaac
    25021 tgctagtcat aaagaaaaag atcaggcaag tcaattgatc gataaacttg aaaacggcga
    25081 agaagaaatt tctgagccag ttgatccaaa ggaaagagaa agacaagatc aagaaaactt
    25141 agaaaagatc ttagatgaac tagcaactga agcgaataaa acttcggcgc gtctagatga
    25201 agtaaatgct caattcgctg acaataaggc agcacgcaat gagaaacagg aagcattgat
    25261 gcagctgaat acaaaagaag aattaggcga gttgacagaa gaagaattag cacagcgtca
    25321 gacgttagaa gcagaaatca aagaattaga agctagtggc gatacactgt tagaagaaca
    25381 attgaaatta gaagcagaat taaaagatat ccgtaagaat caatggtctg agaaaaaaga
    25441 aacgtttact gataagtttg aattgccaga tgattggaaa gatcaagcaa cagatacact
    25501 gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat tcttgaagaa
    25561 aacattccaa actgtttcag aaacagtgaa tgacaacatg gaatggaaag atgtcagctt
    25621 acgtgtccct gggattgcga caacgaaatt tgagcatgaa ttttactatg aagctcctgc
    25681 agcaagtatc cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag
    25741 caacgatgtg aaagttgaag ctaagatcaa actttacgga aaaatgggtg ctgaaccatt
    25801 tgaagcattc tctgaacgga gccagattga ggttaatgaa gatcatatct ccttccaaat
    25861 tccaaataaa cgtgtgcgtg cagaccttgt tttctactta cctaaacgag tatacgacca
    25921 cgcagctatt aaacttctaa atggcaacat catgattgaa acattggaag ctaaagatat
    25981 ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa cgatgctgga
    26041 agtagaaggt gtaaacggca atatcgatat tcgaaacggg aacatcttag attctattat
    26101 cgaaacagta aatggtacag tgacatttgg tgcgacagca gaaaaccttt ctgtgtcttt
    26161 ggtaaatggt gatgtacgtc ttacgattaa agaagataat ttgaaaaaag tagaagcaag
    26221 ttcagtgaac ggaaatgtca aagtcgcttt accagatggc atcggtttgg aaggacatgc
    26281 taaaacaagt cttggaagta tcaacagccg tctgtccaat tacgaagtag ttcgtgagaa
    26341 aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatggtg aaatcgcgca
    26401 agtgcaactt tcaacgacaa caggaagtat ctatttaaaa gatacagata aataaagagg
    26461 ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat tttgtaaaat agcttcccct
    26521 attttacaaa attgtgcctt attgccgata gtctatctct tgaacaccgt ttaccaagag
    26581 attatgaacc tgccaattat cgccgagtca caaggaataa tttcagaaaa agctcgtcac
    26641 gttttatgaa tatggtaatt tgtgaccgag aattttgcag aaagacaccg attaagaaga
    26701 aggttgtcac aacgattttc ttcatttaaa atagtagaac agaggtgaga catgatgaaa
    26761 aaaaagttga caaaatctaa taaaaatgtt gttttgacag ggactttagc aggaatagga
    26821 gaatacatcg ggattgatcc gactgttatc cgtgtgcttt atgtattcct gagcttagtt
    26881 gcttttggaa gtccaattat tttatacatt ttattagctt tgattatccc atttgcgcgt
    26941 tctgcacgaa caaactatgg acataacaat gcatattatg aaaaaaacaa ttatcgtgaa
    27001 caaactcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga tgacgaatgg
    27061 agtgactttt aatgtcgtac tttcaacgta ttttagttaa tacgttaaca ttcatatcat
    27121 tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacggcc gttcttgcca
    27181 gcttcgtatt gtctgtgttg aatatgtttg tcaaaccgat tttgacgata ctgtccctgc
    27241 cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg attcttaaaa
    27301 tgacctcctt ttttgtggga gcagaacatt ttagtttttc ttcattcggt gcagcgttgt
    27361 tagtagctat cttgatgtcg cttgtcaatg taattgtcag cgaacataac agtagaaaat
    27421 aaaagaaagc tgtgccgaac tatcggcgca gtttttttat ttagaatcag gaatgcgttt
    27481 tctattacat tggtaattaa ataaaaacgt tttttttaat tggtagtact tttggctaga
    27541 caatgataaa atgatagaga acactctaat agaaatgagg tagcatgaat gtcagaagtt
    27601 gtaaaagtta gccaattagt gaaaaaatta tctttggaaa tcgtgacagg cgacgagaaa
    27661 agcttagacc gtgtcatcgt aactggggac atctcacgcc caggactaga gttgactggt
    27721 tattttaatt attattccca taatcgttta cagctatttg gcagtaaaga aatcagtttt
    27781 gcggaacgga tgatcccaga agagcgtttg atcatcatga gaagattatg tagtgaagat
    27841 atgcccgcat ttattatttc aagaggatta ccagctccag aagaaatgat ccaggcagca
    27901 aataaaaatg ggatagctgt actgcgttct cccatctcaa cttctcgttt attaggagaa
    27961 ttatccagtt atttagatgg tcgccttgcc cctcgaacaa gtgtccacgg tgttttagtg
    28021 gatgtttacg gattaggtgt actgattcaa ggtgacagtg ggataggaaa aagtgaaaca
    28081 gcgttggagt tgatcaaacg cggccatcga ttgatcgcag acgatcgagt agatgtttat
    28141 cagcaagatg aattaacggt gatcggagaa ccgcctaaaa tcctggaaca tctgattgaa
    28201 atccgaggaa tcgggatcat tgatgtaatg aatctttttg gtgcgagtgc ggtacgtggc
    28261 tctatgcaag tccagttggc tgtttatctg gaagcatggg caaaagataa gaaatacgac
    28321 cgtctgggct ctgaagacac gaccgttgaa atcgcggaag tgggcattcc acaaataaaa
    28381 atccctgtca aaacaggacg aaacgttgca atcatcattg aagtagccgc gatgaatttc
    28441 cgtgcacgta caatgggctt tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg
    28501 attgaagaaa actcaggcaa cgactagcaa ggagtgacaa tatgttagca caaatcaatc
    28561 gagttgcttt tgagcttgta gggattccta tctactggta tgcattgatc atcgtatcag
    28621 gtatcattat cgctatgtgg cttagcagca gagaggctgt tcgggtagga ttaaaagcag
    28681 atgatgtgac agattttatg ttgtggggat tgcctatctc cattattggc gctcgcttat
    28741 attatgtttt atttgatctt ccccagtata tagctgatcc aatccaaata ttcaatatcc
    28801 gttcaggagg cttagctatt tatggcggac tgattgcagg cgcgatcacg ctgtattttt
    28861 ttactaaata tcattttatt tctttgtgga cttttttaga tatcgctgca ccaagtgtat
    28921 tattagcaca ggcaatcggt cgttggggaa atttcatgaa ccacgaagca tatggaccag
    28981 acacgacgcg aagcttctta gaaagtctgc atttaccgca attcatcatt gataatatgt
    29041 atatcgaagg gatctatcgt caaccaacat ttctttatga atcactttgg agtttgattg
    29101 gatttatttt gcttttgttt ttacgaaaga aaccacacct tttgaaaaaa ggcgaagtcc
    29161 ttttatgtta tctaatgtgg tatagtttcg ggcgtttctt tattgaagga ttacgtacag
    29221 acagtcttta ctttttaggt gtgatccgta tttctcaatt attatctgct gtgttatttg
    29281 tcggtacatt gattctattt atttggcgca gaaaaaaagg aaatttaccg gattatgacc
    29341 gctcattagg aaaaaatcaa caaattatct gatttttaga gcgttaaaaa tgaaaaaaat
    29401 ttcaggagtg gtaaaatgaa ttatagagta gcggttttag gaccaggctc ttggggtact
    29461 gctttggcac aagtcctcgc cgaaaacgga catgacgttc ggatttgggg acatcgtgct
    29521 tcacaagtag aagaaatcaa cacccaacac acgaatcaac gttatttacc agatcattat
    29581 ctacccgatt ccatccaagc attcaccaat atggaagatg cagtaaaaga cgcagatgcg
    29641 gtgctattcg tcattccaac gaaagcaata cgttctgttg caaaagaact agtgcctaaa
    29701 ttgaatacaa agccagtgat catccatgca agtaaaggat tagaacaggg aacacataaa
    29761 aggatctctg acatcttgtt agaggaaatc ccagcagaaa aaagaaaagc tgttgttgtt
    29821 ctttctggac caagccatgc agaagaagta gcaaaacatg acatcactac gatcacagct
    29881 gcaagtacag atgcaaaagc agcaaaatat gtacaagagc tgttcatgaa taattatttt
    29941 cggatctata ccaatcctga tgtgatcggt gtagagatgg gggcagcttt gaaaaatatt
    30001 attgctattg gtgctggcgc tattcacggc ttagggtttg gcgataatgc aaaggcagca
    30061 atcatgacgc gcggattagc tgagatcagc cgtttaggtg tagcaatggg tgccaacccg
    30121 ctgaccttta tcggattaag cggaataggc gatctagtcg ttacttgcac aagtgttcat
    30181 tctaggaact ggcgcgcagg caacttgtta ggacaagggc agcccctaga agaggtgcta
    30241 gccaatatgg gaatggtcgt tgaaggtgtg aatactaccc aagcagcgat ggaactagcc
    30301 caaatgatgg aaatcgaaat gcccatcact caaacaatct atgaagtgct gtatgaacaa
    30361 aaagaaatca aaacagccgc aaaagaaatc atgttacgtg atggaaaaaa agaaaatgaa
    30421 ttctcattaa caattttttg aaggagcgaa tgtatatgaa agttaaaaaa gcagttattc
    30481 cagcagcagg attaggaaca agatttttac cagcaacaaa agctatggca aaggaaatgt
    30541 tgccaattgt tgataaacca acaatccagt tcatcgttga agaagcgttg aaatcaggta
    30601 tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat cactttgatt
    30661 caaatattga attagaaaca aacctaagtg aaaaaggaaa gaccgaatta ttgaaattag
    30721 tagaagaaac gacagatgtc aacttgcatt tcatccgtca atctcatcca aagggtttag
    30781 gacatgccgt tcttcaagca agagccttta ttggaaatga accatttgtc gttatgctag
    30841 gcgatgatat aatggaagac gaagtaccat tgacacagca attaatcgat gattacgaac
    30901 gtactcatgc ttctacgatc gccgttatgc aagttcctca tgatgaaact tcaaaatacg
    30961 gaattatcaa tccaggagaa gtattggaag atgggttata caatgtcaaa aactttgttg
    31021 aaaaaccaga tccaagcaaa gcaccaagtg atttagctat tattggacgt tacttattga
    31081 ctccagaaat ttttgatgta ttgtccgagc aacaaccagg agccggaaat gaaatccaac
    31141 taactgacgc aatcgataca ttgaataaaa cacaacgcgt atttgcccgt aaattcacag
    31201 gtaaacgtta cgatgtcggt gacaaatttg gttttatgaa aacaagtatt gaatatggtc
    31261 taacacatcc agaagtaggt gcacctttac gtgagtacat cctgtcttta ggtaaaacat
    31321 tagaagcaga aggaagcaag atcgatcata atccaaaagg aataacagaa atcaaagaag
    31381 aaaagaaaaa ataacccttt tttctaacga gcttcagctg ctaattgaat aaaatgtagt
    31441 tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct atccttatag aaaagagaga
    31501 caagaaggga gtttgatcat gtcaggagga gaaattgcag gattgatcgc agcaatcgcc
    31561 tttgcgatac ttgtcgtttt tatcgttcgt gtgttgttgc aagtttctaa aactttagaa
    31621 aaagtagatc agactgtagc agaagcaaat acgacgatcg aaatcgtcac aaaagacgta
    31681 gatcttttgt ctagacaagt agaaggctta ctggtcaaaa gtaatgaact gctcttggat
    31741 atcaatggaa aagtcgcaac gatcgatcca ttgttcacag cagttgctga tttaagtgaa
    31801 agtgtatcag aacttaatca ttctagtaaa aacatcgcga cgaaagtcgg cggaatcggt
    31861 aaaacgacag ctaaagcgac ggttgccgga aaagtcggtg ggacagcgat gaaatttttt
    31921 aaaaacaaaa aacatactga aacgacagga ggaaaataaa aatggctaaa aaaggtggat
    31981 ttttattagg tgcagtagtc ggaggcacag ccgcagcaat cgccgcgtta ttgttagcac
    32041 caaaatctgg taaagaactt cgggatgaat tagctgccca agcggatgat ttgatggata
    32101 tggcttcaga ttacacagat atggcgaaag aaaaatcgac tgagttaaca ggtattgcaa
    32161 aagaaaaagc gcaagatctt tctcaacaag caggtgatct agcaggtagt gtcaaacaaa
    32221 aagtaagtga atcagcagat gaagcaggaa gtgaatctga cgatatcttg ttaaatctta
    32281 aagaacaatc agctgactta tctaatcgtt tcaaaaaaac ggcagaagat atgaaaaaac
    32341 ctgcagatga attaggaagt attgctaaag atacttcaga agatatcatg attgatgtaa
    32401 aagacgctgc tggtaaagca ggagatactg tcaatgaagg aatgtcagaa gcaaaaccag
    32461 ttgcagatga ctcaaaagaa atgatgaaaa tggcaaaaga agatgcgaaa gaagctaaaa
    32521 aagaaataaa agatacaatg acagattcat ctacaaatca ctaaaatcac aaatttatag
    32581 aataaaaagg tgggagcgga ctagctctca ccttttttac tcttattttt ctaaaacaag
    32641 taattctttt ggtgtagtcg tgaatggcac acagccatcg cttgtcacat atacacagtc
    32701 ttcgatacgt acccctactt tttcaggaat atagattcct ggttcaagag agaagcacat
    32761 cccttcttcg attactaagt catttccttc tacaagggaa gggaattcgt ggacggttgt
    32821 tccgatcccg tgacctaaac ggtgattgaa atattcgccg tagccatagc tgttgatcac
    32881 gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct ggacggacgg cttcttgtgc
    32941 ggcaagctgt gcttcaagag tgatgttgta gattttttct tggaagtcgg ttggtttttg
    33001 ataagcaatt gtacgagtag catcactgca atagccattc caaacaacgc caagatcgaa
    33061 taacacaagt tcattcggtg aaatcttcgt atttcctggt gtaccatgag ggcttgcagc
    33121 atttgctcct gcaaggacta atgtgtcgaa agacatttgt gaaacacctt gttttttcaa
    33181 ttggtattca atttctgcaa tgatcgcttg ttcagtcacg ccttctttca ctgctttaaa
    33241 gccaatctca aatgcaacat cggcccattt accagcttcg atcagtttgt ctttttcctc
    33301 tagagttttg atcagttgta gacgttggat gatggaagtt agatcagtgt aagaatccgt
    33361 tcccgtcaag atatcagaga tagcatcgaa gcgctcgaca gtcaaggcgt tcttttcaat
    33421 ccctacattg ccaggctgac catagcgagc acgaagttct tttccgatga tctcccaagg
    33481 gttttcgctg tctaaatagc cacggacatc atacgcccaa ctactgtttt gagcatcttc
    33541 aacttccaaa gcaggagtga aaaggaaagg atcttgttca agagggataa aaagagctag
    33601 tacacgctca tgaggatcac tttcgtatcc ggaaaagtaa gcgatgtggc cagggtcgct
    33661 gatataagcc aattcgactt ggtgttgtgc catccattga cgtagttcat tgatttttac
    33721 gttattcatt tggcaaccta ctttctaaat taattttgta ttcaataatt gtaccactga
    33781 tttattaaaa gaccaacgtt ctaaaaactg aaaaaagtaa aaattttcat gaaaacagag
    33841 ataaaacggt tgcaattctt gagattattt gatattctat tttagaaatg taagacgtat
    33901 tttcagaaaa catataaaat atgaatagga gaacacaaat ggaaaagcaa acaatcacta
    33961 tctatgatgt agcgagagag gcaaatgttt cgatggctac tgtttcacgt gtcgtaaatg
    34021 gcaatccaaa cgttaaacca gcaacaagaa aaaaagtctt ggaagttatc gatcgcttgg
    34081 attatcgtcc aaacgcagtt gcgcgtggct tggcaagtaa gaaaacaaca acagttggtg
    34141 taatcatccc tgatgtcagc aatatgttct ttgcttcttt agcacgtggt atcgatgatg
    34201 tggcaacaat gtacaaatac aatattatct tagcaaattc agatggcaac gaccaaaaag
    34261 aagtcaatgt attgaataac ttactggcta agcaagtgga cggcgtgatc ttcatgggtc
    34321 accatatcac agacgaaatc cgtggagaat tttcacgttc aaaaactcct gttgtattag
    34381 ctggctctat tgatccagat gaacaagtag gtagcgtaaa cattgattat gtatccgcaa
    34441 caaaagatgc agtaaacaaa ttggcaaaaa gcggcaataa aaaaatcgct tttgtcagtg
    34501 gtgcgttgat cgatcccatc aatggacaaa atcgtctgaa aggctataaa gaagcattaa
    34561 gcgaaaataa cctaccatat agcgaaggat tgatctttga agcacaatac aacttcaaag
    34621 atggtctatc tctagtcgat cggatccata atagcggtgc aacagctgtc tatgtttcag
    34681 atgatgaatt agccatcggt attttggatg gtttattaga ccgtggcgtg aaagtaccgg
    34741 aagaattcga aatcatcaca agtaacaatt cactactgac agaagtagct cgtcctcgtt
    34801 taacaagtat cacacaacca ttatacgatt taggggcggt atctatgcgc ctattgacga
    34861 aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat cttgccttat ggaatcgaag
    34921 aaaaaggatc tacaaaataa taaaaggaca accattcaga ggttgtgtgc ctgccgttta
    34981 actctgcgtt acaaggagca atttcaaaaa acagcatctc atgtttgatg aatattgtga
    35041 ctcgtgattc ggagttggca ggagaacacc gtttatttgg gaaaaggggt tatgacaaaa
    35101 ttttgttata atcctttttt tgtgtcttaa aaaagagaga aagtggttga aattacattt
    35161 atcgtatata aaacaatccg tgtatagatg ctgcattttg ttcaccacca tcaaaaaaat
    35221 aagggactgt gacaatacta ttgtcacaat cccttattac tgtgtattct attaaatcaa
    35281 tcatctttct catctgattc tttagttgct tgattcgctc ttgcataagc tgaagcggtg
    35341 ttccagtaat ctgtatagtt ttcatcggta ccgccaacgc caaatttaaa ggcgcttttc
    35401 ttcggtccat ttttcgccca tagcgattca actgtttttc ctggtgtttg gatggatttt
    35461 ttattgacag taacttttcc aggtgtttga ccagtaaatc cagatacttt tttcttcatc
    35521 acatcagatg ataattcgaa tttctcatca gtgccaaaaa tgtcaggttt tgcttggtaa
    35581 gcttgattga tcagattggc aagataatta gccgaacgta tccttgcttt gctgtccatt
    35641 ccagtattgt catcgtgccc tgcccagcta ctgatggtaa tagacggagt agagacaatt
    35701 agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc tgcttttcct
    35761 agatttccgt ttaagcttga gatggcatct ttgaatggtg tagtgatttt tgcattgatc
    35821 acactacgca tcatgtcatt catgatcgat gccgttgctt ccgaatagat ccggacagag
    35881 ttgctttcgt gtttataaat tacatttcct gcgttatctg tgatggaatc gatgatatag
    35941 ccttcttcat agacgccgcc gtttgctaaa gcctgaaatc cgttggtctg ttgaagcgtc
    36001 gtgacatcta ctgtacctaa aggagcagat tcaacgcccc aattgtcatt tgccgggtag
    36061 ttcatctttg ccaagtagtg ctcgtaagca tattgtttcg atccaccgga attcaacaca
    36121 tcttgatata agtgataagc cgggatattg ttggaccatt ctagtgactc acggacggtt
    36181 tggaaggtgt tcgatccttc gttagtagca ttgacgattt tctcgccagc attttctcct
    36241 tcttgccaag ttgttgcata atctgataca cgggattctg atccgataag ccccatgtcg
    36301 attgcaggtc cataaacaag aactggtttg attgatgagc cagcttgacg ttctgtatcg
    36361 aaggcatggt tattttgatt ctgactgtaa ttccgtccgc caacgaatcc gtagatgcgg
    36421 ccagttctgt tatccatcaa tacatttccg gtttctactt gactgccagc accatcatct
    36481 aacatgtatc cataattttc aacgccattt tgcaatgcag catagacatc tttatcgatt
    36541 gtcgaatgga ttgtatatcc tttattttgg atcgtttgct gtgccttttg ataataagcg
    36601 tcactgaccg atgaatcaga catatcagcg ttgtcttttt cagccaattg ttgtgcaatg
    36661 attcttgttg cttcattcat cacagtataa tacaagaatt ctctgtcagt ctgttctgcg
    36721 atttgttgtg gtagaaagtc ttttgtaagg tcataggctt ttgcttcttc gtattgttct
    36781 ttcgtaattt ggccttcacg atacatactg aataaaacaa aatcttttct ggcaaggccg
    36841 gcagaaagat cttcttttag cgcgcctgtg ttcgtgtatg gactatacgt gatcgggctt
    36901 tggggtaatc cagcgatata agcagcttgc ggcagcgtca catcttttgc atctacacca
    36961 aagattcctt ttgcagcttc ctggactcca gcaatgtttt gtcccttatt attacgacca
    37021 aatggtgaga cattcaaata cgtagcaatg atctcatctt ttgaaaagta tttttctact
    37081 tgtgcagaaa gaatgatctc gtttgcttta cgtttgaatg ttgtttcatc tgtaaggatc
    37141 tgctgtttga ctaattgctg tgtcaaagtt gatccaccag aagagccaat accagtagct
    37201 tcagatacta atgctcgtaa aacagcttta ggaacgtaac cttggtgttt atcgaagtat
    37261 tcatcctctg tgctgataat tgcagttttc aataaaggtg aaatcttatc agatgaaata
    37321 gtcgtgcgca tcagatctgt ctggattttt gatatctctg tattatctgc ataaacaatc
    37381 tttgacgttt ctgtaatgtt tcctagttct gtctgcagtg cttttttagt cggcaattgt
    37441 gtgtcttcga cgagataggc aaaatagcca gcgcctattc caatacctag agctgcgaac
    37501 atcaaaagaa gaacagtaat aaaaacagtc aatgattgaa aaactctaaa aataatctta
    37561 ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt tttgcgtggc
    37621 aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa tagaacaaaa
    37681 aaacagacaa ggctgttttt ggcaggaatt tcttttttaa aagagttaaa acgtgagaaa
    37741 atgatcttta ttataaaaga gttcttaagc ttttctttag agaaaatttc agttactatg
    37801 gagaacaggg caaagaaaaa tcaatcattt tctttgtata tcatcttacc aatatatagg
    37861 aattgttagt aacaattgcc tttcaaggag gaatccatcc atgttattag gctcacacgt
    37921 cagcatgagc ggaaagaaaa tgctgttagg agcagcagca gaagcagcca gttatggatc
    37981 ttcgactttt atgatctata caggcgcccc tcaaaacact agaagaaaag ccatcgaaga
    38041 gatgaatatt gatgccggaa aagcgtacat ggcagaaaac ggtctaacaa atatcgtcgt
    38101 tcatgcccct tatatcatta atttggggaa tacgatcaaa ccagaaatgt ttccttttgc
    38161 cgtccaattt ctgagagaag agattgttcg tgcggaagct ttaggcgcta ctcaaattac
    38221 acttcaccca ggtgctcatg taggggcagg aacagaagcc ggaattgcta gaattattga
    38281 aggcttgaat gaagttttga caaaagaaca gacggcacaa atcgctttgg agacaatggc
    38341 aggtaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca tcgatggagt
    38401 aaaattaaat gaaaaattat ccgttacttt tgacacctgt catacgaatg atgccggtta
    38461 taatattaaa gaagactttt caggtgtaat ggaagagttt gataagatca tcgggttaga
    38521 tcggcttaaa gtactccata tcaatgattc taaaaatccg caaggttcgc ataaagaccg
    38581 gcacgccaat attggttttg gaacaatcgg attcgacacg ctgaataaga tcgtccatga
    38641 tccaatgttt gacaatattt cgaaaatctt ggaaactccc tatgtcggca aagataaaaa
    38701 gaaagcttac gcaccatatg gaaaagaaat cgccatgttc aaagcacaaa tattcgatcc
    38761 agaagtgttt cctgagttat tagcagaaga agtgatactc taagattttc tatctgacag
    38821 cagcaaaaaa ggaccaggca cattgccttg tccttttttg tttaagaaac aaaggagtac
    38881 tttttgcagt ctttttcatc atgcatcttc tcccccctct ttttaaattt agacaaacaa
    38941 ggtatactgt tagaaaagta aaaaaacgaa aagtttcaca agcaagtggc aaaacgtgat
    39001 tggaaggacg aaaagatgct gaacacttta aaacccttga tcaaccaact aaaaaagaaa
    39061 catacatact ttattcttgg catagcccta ttatttacaa ttattcgttt gtatttatat
    39121 agtaaagcaa tctatggaac agacatgtcg gcaacctatg atgaccagct gctgattcgc
    39181 tatgctcaaa atattttgga cggtaaatgg ctaggggagt atacgactac aacactttcc
    39241 aaaggaatct cctacagctt atttttagta cttgcacata aactgcatct ttcttatgct
    39301 gttttgttcg gcatgttcag tatttttgcc agtatattgg ttgcactcgc gtttaaaccg
    39361 atcgtgaaga acaagtatct tcttttaagt atctattttt attttttgtt ttctccgatt
    39421 agttttacac atgaatattc cactcgaaca tatcgtaatg ccctcgtgat tccggctgtt
    39481 gagatcatta tcgcttgttt actagctatt tattaccgca aatacggttc tagcagaaaa
    39541 ttgctgccat ggtttttagg gctttctgtc gcttttcctt tcttttggtt cattcgcgaa
    39601 gattctatct ggcttcttcc ttttactgta gtagctttgg gtatcagttt cgtccagatc
    39661 ttgctagggc aagacttttc tttcaaagaa gtaaagaagt taaaaaaacg actctttctt
    39721 ctctcgaaac gggaatggct gaaactttca ttgtttttac ttcctatagt tggcttttcc
    39781 atcgtctatc agtggatcga atataaaaat gagacaacat acggtattgc aaccatcaat
    39841 gaccgttcca gtggagaatt tggacgtttg atgaaacaac tgatccgaat agatgatgaa
    39901 acgaacttga ataaagaaaa cagtgagata tgggtctcac atgctgcact aaataaagca
    39961 ttagccgcat ctcctacatt ccatactgtt tctacccgta ttgacgaatt atatacgact
    40021 cacgcgtgga ctcaaggtgg aaaagtaaaa gaattacccg gagatatcat cttttgggca
    40081 ttgcgagatg tggtaagcga atctggttat taccaaaaca atgcaaaaga aaccaatgat
    40141 ttttggaaaa aagtcaacca agaattagaa gaagcctatc acaagggaac catcaaaaag
    40201 aaaaaagagt tctatctgat gggatcagga gatggaaaga cgatgagtga tctgccaatc
    40261 gtattcgatt tccttcaatc aggatacgct tatgcatggc gatataaaga tttccatcaa
    40321 ggaggaaatt atagtttcgg aacgcaagaa caagtcaaac aggctcaaga attattaaac
    40381 cttccgctaa tggagaattg gatcgatagt aatgaaccga gaacacctaa tttccaatta
    40441 acaaaatcag caaaagtcgc gaatgtcatc ataaaaattt accaagttct ggctattccc
    40501 gtgctgattg cttcaatagc agggatatta cttatgatac taggaaccgt atttgcaaaa
    40561 gctgatcgtg cctattatag ttcctctttg attgtggtca ccggattaat actgacacaa
    40621 ttggtttttc ttttcggtgt ctcgtggttt tgttcttatg cgccagaaaa aaaggattat
    40681 tttttaagtg tctacactgg cgcaggcgtg ccattaacgc aattcatcgt agtaacaagc
    40741 atgctagttt tcagtaaatt aaatctgaaa aaggctttat cttctaaata aaaagaccct
    40801 tggtagctag atttttaacc taactatcaa gggtctttgt gtggaaaaat cagtttctat
    40861 tcgatttctt ctaccgtttc ctgcaaacgc tgttcatatt ttttgtttcc tttatataaa
    40921 tcaccaatcg tccatgccaa caggagaagg acaagaacaa tgattacata agcgatgtga
    40981 tcagccaatg ctagttgagc agaagtggct tgttcaccaa agaaactttc gatctgtcct
    41041 ggtaatcctc gtaagttcaa gtaagttaga ctaatcacag agaaccagcc aagaagtttt
    41101 acgatgaatg aatttttaaa tcggctaccc atctccgctt ggctgttggt catcatcagt
    41161 aaagggatca tcgaaaacgg caacgcaaaa gccaagaaca cctgggattc attcatcaag
    41221 ttattcaaag cggtatgttc ttcgatcgtt ccttttgtac ttgtaaacaa aacacaaatc
    41281 aatacaggaa tcacagaaag caatcgtgtg accagtcttc ttagccaaat cgggatacgc
    41341 atatggatga agccttccat gatgacttga ccggtcaacg taccagtaat agtagagttc
    41401 tggccagaag caagaagtgc tacagcaaat aaagtggata agataccaga ttttgctaca
    41461 gcaatcaaga taccattgct catagctgat ggatcagata aggcttcata cagaccaaaa
    41521 aaggaaggat cttggacaga accggttttg aagacagcga cacccatgat caatagcagt
    41581 gcattgacta gaaaagccat cgacaattgg atattagagt cccaagtaga aaaacgtaca
    41641 gcttgtgcta catcttcttc gtcttggtga tcaatcttcc gagtttgaga aactgctgaa
    41701 tgaagatata agttatgcgg catgactgtt gcaccaataa tccctaaagc tccagtcagc
    41761 ggagtttcgc cgccgatgac cggtgagtct gagaatgtat gactgttagg gataaagcca
    41821 gcaaataaac tgtgccaatc tgggttagac aagccaactt gatagacaaa aacgaataaa
    41881 atgacgaaaa ttaagcagac aaccagtgct tcgatcttac gaaacccgac ttttgtcagt
    41941 agtaagagaa cgaaaacatc taatacagta ataaagacgg caatcaccaa tggaatatca
    42001 aaaagtaagt agagggcgat tgctgcacca atgacttctg cgatgtctgt tgccataatg
    42061 gctaattcag tcaagatcca taaaatgaca cccaatgtct tacttgtgcg tgctcggatt
    42121 gcttgggcca aatccatttg ggtcacgatt cctaattttg ctgccatata ctgtaaaagc
    42181 atagcaatca gactagacat caaaataact gacatcaata aatattggaa attttgtcca
    42241 ccagtgatcg aagtggacca gttacctgga tccatataac caactgctac taatgcacct
    42301 ggtccagagt aagcaagaag tgttttaaaa aaacttttcc cctttggtac atcaactgtt
    42361 ccatttactt cagctaaaga taaaccattt gtatgttcaa taagtcgatg cttatgaaat
    42421 tgtttgttgg attgattatt caagaaaaaa ccacctttcc tatttatcta cgattacatt
    42481 ctacacctca aaaagaaaaa attaaagaaa aatattcggt atgcctaatt tattttcgtg
    42541 gcgcgtcttg ttagatgacg aaagaaattt tgttctcctc taaaaaaacc actatttttt
    42601 gcatgaaact aaagaatacg cctttctttt ttcaataaac tttagtaaaa taagtcattg
    42661 agagcaattc ggaaatagaa aggagagatt tatatgttat caacaggtat tgtcgtactt
    42721 attgctatta ttgctttgct aatcggcgca gtaggtggct ttttcctagc gcgtaaatat
    42781 atgaaagact atttagaaaa aaatcctccc gttaatgagg acatgttgcg atcaatgatg
    42841 atgtcaatgg gacaaaaacc atctgagaag aaaattcgcc agatgatgca gcaaatgaag
    42901 aaccaaggga aaaaataggt tctgacccaa cgacttttta cgggctgatt ttttcagtcc
    42961 gttttttatt ttgtaatttg ttagactatt cagattattc agcaaaaaga caagcacaga
    43021 caatcctagg ctcttgataa agacaaagga ggagtaaaat gtcgattttt agaaagttag
    43081 gctggttttt tcgtcaggaa aagaaacatt atttaattgg ggttttctcg ctattcgcag
    43141 tggccttggt acagttagtt cctccaaaag tgattgggat catcattgat gagattgccg
    43201 ataaaaattt atcagtaaag atcattttcg ggtggatctt cctattgatt atcgctgctt
    43261 tcttacaata tctttttcgt tatatctggc ggacgaatat ttggggaagt gcagctcggt
    43321 tggagaaaga acttcggcag cagttattca aacattttac aaaaatggac aatctctttt
    43381 accaaaaata tcggactggg gatttgatgg ctcatgcaac gaatgatttg aatgcgatcc
    43441 agaatgtagc aggagctggt atcttgacct ttgcagattc agtgattacg ggcggaacaa
    43501 cgatcattgt gatgattttg tttattgatt ggcgcttaac actgattgcg ctgattcctc
    43561 tgcctttgct ggccgtcact tcacgagtgc tggggtcgaa actccatgat gcttttcgtg
    43621 attcgcaaga agctttttct agtatcaatg ataagacaca agaaagtatc actgggatca
    43681 aagtcattaa aacatttgga caagaaaaag aagatctagc ggattttgaa gaaaagattg
    43741 aagatgcaat tattaaaaac aaacgagtca attttctaga ctccttattt gatccattca
    43801 ttaccttgat tatcggtctg tcttatgttg caacaattat tttaggtgga aagtatgtct
    43861 tggaaggcac gatctcgatc gggcagatgg tctcatttat tagttatatc ggtatgcttg
    43921 tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt ggaaagagga aatgcttctt
    43981 atgatcgcgt caatgaactt ttgcatgaaa aaacgcatat cgtggaacaa ctgggtgcag
    44041 tgcagacccc ggtaaaaggc gagctgaatg tagctatttc ctcttttaat tatccgaaag
    44101 aagaatcacc agcattagaa caaatcaagt ttgcaatcca agaaggagaa acattaggta
    44161 ttgttgggaa aactggtgcg ggaaaaacaa cgatcatcaa attactgatg cgtgaatacg
    44221 atgattatca agggaaaatc caatttggcg gacgttcgat caaagaatat tcattgaatg
    44281 ctttattagg atcaatcggc tatgtgcctc aggatcactt cttgttttcg atgaccattc
    44341 gagataatat ccgtttttct aatccaaaac tttcagaaga agaagtagag catgcggctt
    44401 ctttagcatt tatcaacaat gaaatcaagg aattaccaaa aggttatgat accttagttg
    44461 gagaacgtgg agtctcttta tctggcggac agaaacaaag gatttcgatt gctcgagcat
    44521 tgatcaaaaa tcctgaatta ttaatattag atgatgcact ttctgcagta gatgccaaaa
    44581 cagaagaagc tatcttgcat aatttaaaag aaataagaaa agagaaaaca actatcattg
    44641 cagctcaccg tttaagtagc gtgatgcatg caaaagaaat cattgttgtc gatcaaggga
    44701 aaatcattga acgaggaacc catcatgagc tgcttgcact aggcggctgg tatgaacaaa
    44761 tgtgggagaa acagcaatta gaagcgaaga tagaaggagg cgaggcctga tggatgaaaa
    44821 ttatcaatcg gaatggacaa aatcgattcc acttaaagaa cagaaaaaga tcatcaaacg
    44881 actgatgaag ttcgccaaac cttttagaac aacgttcatc gttgctatct tatttgcgtt
    44941 tgctctgtct gtgatcaatg tcctattgcc aaggatcatc caaactttta tggatgatca
    45001 tctggctaaa cagtcggcta cgacacaagt tattttgttt tttgctggta tgtacttatt
    45061 tggagtccta gtaaaaagta tcatttggtt tttccaatgg tatttgtatt ccatggcttc
    45121 attgaagacc tatcagtata ttagggtaaa attatttgaa aagctgcata cactaggtat
    45181 gcgttatttt gatcagacac cggcaggatc aaccgtttct cgagtgacga atgacacaga
    45241 aacgctattt gaattttggt acgtcttttt gatggtattg actgggatct ttgcagtcgt
    45301 ttcttcattc attgccatgt ttcaaatcaa tggagaaatt gccttgtgga ctttggtgtt
    45361 cttgccgatt ttaggaattg tcatctggta ttaccagaaa tttagttctc gtatttaccg
    45421 aaatatgcgg gaaaaattaa gtcagctgaa tacaaaattg aatgaatcga tttccggcat
    45481 gcgtattatc cagcagtttc gccaagaagc tcgtttagct aaagaatttg aacaagtgaa
    45541 caatgagtat ttagacacgc gctatgcgat gatcaaaacg aattcgattc tgttaagtcc
    45601 gatcatcaat tttctatatg cattggcgat tgcgttggcg ttgactttat ttgggatcga
    45661 tgcgttgcat tctcccgtag aagttggact gatctatgca tttacgactt atgtccaggg
    45721 attttttaat ccaatgtctc aaatgatgga ctttttgagc atcttcacag atgggatggt
    45781 tgctggtagt cgtatcttga agattatgga tacggaagaa tatgcaccac aacaaaaccc
    45841 tggagcagat caaatgatta cttcaggtaa gattgagttt cgcaatgtaa gcttttctta
    45901 tgatggagag cacaatgtat taaacaatat ctcttttgtt gctaatccgg gagaaaccgt
    45961 tgcattagtg ggacacaccg gaagtgggaa aagctcgatc atcaatgtgt tgatgcggtt
    46021 ttacgaattt tatgaaggtc agatattgat tgatgatcat gacattcgag aatatccaat
    46081 ggaagagtta cgtaagaaaa tggggctggt cttgcaagat gctttcttgt tttatgggga
    46141 tatcgcagga aatattcgga tgatgaatcc agctatcaca gacgaacaga tacgccatgc
    46201 ggctgagttt gttcaagcaa acaaattcat cgatgaatta ccaaaaggat atcactcaaa
    46261 agtgatcgaa catggtgcca gctattctag tggacaacga cagctgatca cttttgcgcg
    46321 aacaatcgtg actgatccta aaattctagt attggatgaa gcaacagcaa atatcgatac
    46381 tgaaacagaa ggattgatcc aagaaggatt ggcgaaaatg cgacaaggac ggacaacgat
    46441 tgcgattgcc catcggctgt caacgattcg agatgcagaa ttgattttag ttttagacaa
    46501 aggtcatata gtagaacgcg ggaatcatga aacacttcta gcgcaaaatg gtttgtatgc
    46561 agatatgtac aaactgcaaa gtagtggtat ataaaagatg ttgtgacagt agcattttga
    46621 cctaagcact agagtgaaaa taaaacatag cttcccatat ttttcatctg tgagagttag
    46681 tgtcgtagtt accagattta aaaaagacga cgtcagctag tcagaagtgc ctaatagccc
    46741 ctaagaaagc gttgacaaaa tcgtttgctt tcgataatat aaagaaaacg atacaacttg
    46801 gatagtgatt gcctgatgca agctaaacct gatgagtctc ccatggtttc atggacggct
    46861 ttttgggttt ttctttttgt tttggataca gccctatata tcatggaaag gagcaaaaca
    46921 tgctgattga gaaagtactg aataacaacg tcgtaataac aagggatgaa agagatcggg
    46981 aaatgattgt gatgggaaaa ggattggcat tcaaaaagaa ggtgggtgac ccgattcttg
    47041 actcatgcat cgacaaaaaa tttcagttga caaatgacgg actttcacaa cagttccaag
    47101 aattggcaca agcgattcct cttgagtact tagaaatttc ttgtgaaatc attgaatata
    47161 caaaagaggc tctgcaagtc catttaaatg attctgtgta tatttcattg acggatcact
    47221 tgtatacagc tatcgaacgt caaaaaatgg ggatctttgt gccaaatgta ttgctgtatg
    47281 atatcaaaca actgtttcct aaagagtatg ccattgggaa aaaaacagtg aagaaaatct
    47341 cggataaata ccacatcaag ttatcagaag acgaagccgg atttatcgca ttgcatttag
    47401 tcaatgcaca aggcattcaa agccagcaag acaataccta cctgtttacc acaacaatac
    47461 aggatatttt aaatatcgtc cgctattact ataagattac atttgatgaa gattcagtcc
    47521 acttttttcg attcgtcaca cacctgcgtt tttttctgac acgtgtccta gatggagcga
    47581 aatcagaaga tggagaagtt tctgaggaat tgttccaagt tattcaaaaa aaatataagg
    47641 atacgtttgg ttgcgttgag aaaatagcag actttttaac cggggctttc gactaccaga
    47701 tgtccacgga tgaaaaaatg tatttaacga tccatactgc gcgtttagtg catgagaatc
    47761 gttgtaaata aataaaaaag gaggacaaat taacaagaac gtatgaaata agggtagtga
    47821 cattcagttg gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca
    47881 ggaattagca gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac
    47941 acactgtgtg acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt
    48001 tttgaaaaag atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt
    48061 aatcgggaat catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga
    48121 tgaagcagaa gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt
    48181 aagtggctgt ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt
    48241 aaatgctttg ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct
    48301 gaatgggata ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc
    48361 gaaaaaattt catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc
    48421 gactgtacaa ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag
    48481 cttgctgggt cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta
    48541 tactagtagc gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat
    48601 tgccaagaaa tggattcctg cagtcgtcca gacctttttg gtttcttttt ctgtgttatt
    48661 aatcgcactt cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct
    48721 attaagcacg ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct
    48781 agggtttctt tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc
    48841 gattatgcag ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag
    48901 ttttgcacag acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa
    48961 ggcgctttgc atgccagctg tgatttcagg aatcttcggc gtaacagaac cggctattta
    49021 tggaatcacc ttaccgaaga agaagccatt tatttactcg atgattggtg gtgctgtttc
    49081 aggaatctat ttgatggcat ctggaacaat cagctacata atgggcggat tagggatttt
    49141 tggggtggta aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg
    49201 cattgctatt gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc
    49261 aggtgaaaca gatgctatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca
    49321 aggacaaatc gctccactaa atgcagcgaa ggatgcagct tttgcacaag gaacactagg
    49381 tcgaggaatc ttaatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt
    49441 tatgaccctg ttcccgacga aacatgcaat cggaatggtt tctgaaacag ggctggaatt
    49501 gctgattcat gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt
    49561 gcagcaagga gaaagagtga gacaaggcga tctgttgcta cgcttcgaca aaaagaaaat
    49621 cgaaaaggaa ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt
    49681 ggatattttg gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact
    49741 gacctgaacg gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt
    49801 tattttcaaa attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt
    49861 tttgaccgac agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac
    49921 tatataacac gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg
    49981 aatacagtcg ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg
    50041 aatcaccgat actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg
    50101 tacgaaggat cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcgctcg
    50161 tacaattgta cgcttcatag aatgattcac caaaagtggt gacaattgtg acatccattt
    50221 tgatgcgctt cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga
    50281 agatcacgat tttttttgcc tggagcagta aatatttagc tttttgcagt tggtcatgat
    50341 gaatgagaga aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga
    50401 ttgtcataat accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc
    50461 cttcaagata ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac
    50521 ccattttttt tgctacacgg atgagtgtag acgggtggac ataacatcgc tcagctatcg
    50581 cttggatggt catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat
    50641 tttctgaagg agaaaaggag atttctttga ttttttcacg taataacatg tagatacctt
    50701 cttcttattt ttttacgtaa attataactt ttttgaaaat gttttgtaaa cggatttata
    50761 ctatataact taaaataggc tttttttgac aagatattta caaaaatcca ctagaaaaca
    50821 gtaacttttg taaatgttac tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa
    50881 aaagataatg aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc
    50941 gctacgattg gtggaggcag cagttacacc cctgagttga tggaaggatt tatcaaaaga
    51001 tatgaggaat taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag
    51061 cttgagatcg ttagtcagat gtctcagcgt atgtgggatg cttctccata tgaagtcaag
    51121 atctatgcaa ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag
    51181 tttcgtgtcg gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg
    51241 atgattgggc aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca
    51301 gtcgttttgg caatcgttga ggacatgaag cgtttatgtc caaatgcttg gctgatcaat
    51361 ttcgcgaacc caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa
    51421 gtgattggat tatgcaatgt gccagtgatg gcgatgatga cagaaccagc aatgcttggc
    51481 aagaaaaaag aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa
    51541 gtagcagata ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa
    51601 gataatggat taccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa
    51661 atgaacatga tcccttgtgg ctaccaccgc tattactatc gtgaagaaga aatgctggaa
    51721 catgctttag aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa
    51781 gcagagctat ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa
    51841 aaacgaggtg gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga
    51901 aataaagaaa cacagatcgt tgtatctaca aagaatgatg gagctgttcc tgacctgtcg
    51961 gcagattgtg ttgtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg
    52021 tttggttctc ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg
    52081 ctgacgatcg aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc
    52141 aatccactta tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca
    52201 caccagcagt atttaccgca attcaaggaa acaatcagcc gattaaaaga agaaggaatc
    52261 gaagttcaag atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta
    52321 taatggggtt cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg
    52381 gcgcttatga tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag
    52441 accgttttcc tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt
    52501 atccagcaaa aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg
    52561 cagaaatggg gttcaagact tatcgaatgt ctatcgcctg gagtcgtatt tttccaatgg
    52621 gggatgagaa agagccgaat gaagctggct tgaaatttta tgaggatgtc tttaaagaat
    52681 gccaaaagca cggaattgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt
    52741 tgatcaaaga atatggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt
    52801 gtcgagtcat cttcaatcgc tataaaggac ttgtcaaata ttggctgaca ttcaatgaga
    52861 tcaatatgat ccttcatgca ccatttatgg gcgcagggct gtattttgaa gaaggagaaa
    52921 atgaagaaca aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa
    52981 cgaagattgc tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag
    53041 catattatcc atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag
    53101 aaaattattt cttcattgat gtccagtcac gaggagaata tcccgcttat gcattgaaag
    53161 aaatggagcg aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag
    53221 aacatactgt tgattttatc tctttttctt attattcttc acgagtttcg actaccgaca
    53281 acgaactctt ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc
    53341 aagcaagtga atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata
    53401 tttatgatcg ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt gcagtcgata
    53461 cgccagatga aaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata
    53521 tccaggcgat gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt
    53581 ggggatgtat cgatttagta tcagctggta ccggtgagat gaagaaaagg tacggattca
    53641 tttatgtcga tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat
    53701 tcaactggta taaaaaagta attgattcaa atggagaaaa tctttcgaac aattgatcaa
    53761 tgatttataa aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag
    53821 aaacgcaagc agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt
    53881 aagtaaagga gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga
    53941 tgactgctgc tttgtatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg
    54001 cccctggcgg ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga
    54061 tcatgggccc cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa
    54121 atgcttatgg aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg
    54181 aagataaaag ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat
    54241 taggcgtacc tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg
    54301 atggtgcgtt tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag
    54361 aagaagccat ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg
    54421 aattacgtgc acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta
    54481 tgtgggatac agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag
    54541 cacgtaatgt caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg
    54601 ttggactgga tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct
    54661 ggattccaac agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg
    54721 ttcgtgaaaa agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac
    54781 aacaagtgta taaatacttg gagtctttag aagaaacaac agcataagtt taaaaagagt
    54841 gaaagaacgg tttcaattgt tcttttactc ttttttttcg cgtgaaatcc actgaatccg
    54901 atttatgtga atacttgcaa tcatgcttga taaaagaatc ctttcttgat ttaagcgttt
    54961 tttggtcctc atactttcat atctaataag tgaatgctat acttattaag aaccatattt
    55021 cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag
    55081 aaaatttaga agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac
    55141 ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag
    55201 gacctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac
    55261 gtttcatgga tacacaaggt gaagaaacaa aacgccgagg tgtcgctatc gcttatgact
    55321 cacgccatca gtcaccagaa tttgcaatgg aagcagccaa aacactggca catcacaata
    55381 tcccgtctta tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc
    55441 atttgaagac gtttgcggga atcatggtaa ccgcatctca caacccagcg gcttacaatg
    55501 gatataaagt ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga
    55561 cgaaatatgt ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag
    55621 ctaaacatag cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag
    55681 aaatcaaagc agtgacgatt gatcgtgaat tggttgaaac aatggggaaa gatttgaaac
    55741 ttgtgtacac gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag
    55801 ctggattcga acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta
    55861 cggtcaaatc gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag
    55921 aaaaagaggg agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag
    55981 cggttcgttt gccagatggt tcttatgaag tattgactgg gaaccaaatc ggagcattgt
    56041 ttgctcaata tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg
    56101 tattgaaatc aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta
    56161 ctatgttcaa tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caatttgaag
    56221 aagaccattc acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac
    56281 cttttgttcg cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt
    56341 actataaaaa acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg
    56401 gctatttcgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa
    56461 tctctgcttt gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag
    56521 ttattcaaac agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa
    56581 aactaacaac accgccatcc gacgtattga agtatacatt ggaagaaaat ggctggatcg
    56641 ctatccgccc ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct
    56701 ctacagaaga cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag
    56761 aagaataaaa aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt
    56821 caagttggag gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca
    56881 attattcaaa atgttgagtg accccactcg tcttaaaatt ctgctatatc tgaaagacgg
    56941 ggaacaaaat gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca
    57001 tcaactacgt ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat
    57061 tttgtatagt cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt
    57121 ggaacatcaa taacgatagg tgcaaataga taaacactgg agtgtgac
//
LOCUS       DAYYHS010000008        56962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000008 DAYYHS010000000
VERSION     DAYYHS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.008"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(166..669)
                     /locus_tag="ACHA6A_000466"
     CDS             complement(166..669)
                     /locus_tag="ACHA6A_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8968701.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1028..2053
                     /locus_tag="ACHA6A_000467"
     CDS             1028..2053
                     /locus_tag="ACHA6A_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8968702.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2376..3254
                     /locus_tag="ACHA6A_000468"
                     /pseudo
     CDS             2376..3254
                     /locus_tag="ACHA6A_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            3730..3846
                     /locus_tag="ACHA6A_000469"
     CDS             3730..3846
                     /locus_tag="ACHA6A_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8968703.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4774..5112
                     /locus_tag="ACHA6A_000470"
     CDS             4774..5112
                     /locus_tag="ACHA6A_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968704.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5206..5436
                     /locus_tag="ACHA6A_000471"
     CDS             5206..5436
                     /locus_tag="ACHA6A_000471"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8968705.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5836..6207
                     /locus_tag="ACHA6A_000472"
     CDS             5836..6207
                     /locus_tag="ACHA6A_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8968706.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6481..7173)
                     /locus_tag="ACHA6A_000473"
     CDS             complement(6481..7173)
                     /locus_tag="ACHA6A_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8968707.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7176..7961)
                     /locus_tag="ACHA6A_000474"
     CDS             complement(7176..7961)
                     /locus_tag="ACHA6A_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8968708.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8198..9598
                     /gene="lacG"
                     /locus_tag="ACHA6A_000475"
     CDS             8198..9598
                     /gene="lacG"
                     /locus_tag="ACHA6A_000475"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8968709.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9623..9940
                     /locus_tag="ACHA6A_000476"
     CDS             9623..9940
                     /locus_tag="ACHA6A_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8968710.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            9953..11626
                     /locus_tag="ACHA6A_000477"
     CDS             9953..11626
                     /locus_tag="ACHA6A_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8968711.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11640..13271
                     /locus_tag="ACHA6A_000478"
     CDS             11640..13271
                     /locus_tag="ACHA6A_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8968712.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(13762..15327)
                     /gene="guaA"
                     /locus_tag="ACHA6A_000479"
     CDS             complement(13762..15327)
                     /gene="guaA"
                     /locus_tag="ACHA6A_000479"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8968713.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15386..16309)
                     /gene="coaA"
                     /locus_tag="ACHA6A_000480"
     CDS             complement(15386..16309)
                     /gene="coaA"
                     /locus_tag="ACHA6A_000480"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8968714.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16505..17110
                     /locus_tag="ACHA6A_000481"
     CDS             16505..17110
                     /locus_tag="ACHA6A_000481"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8968715.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFSNAEIVTKDKGYYIIKSVKE"
     gene            17418..18434
                     /locus_tag="ACHA6A_000482"
     CDS             17418..18434
                     /locus_tag="ACHA6A_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8968716.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18575..19876
                     /locus_tag="ACHA6A_000483"
     CDS             18575..19876
                     /locus_tag="ACHA6A_000483"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8968717.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            19922..20584
                     /gene="deoC"
                     /locus_tag="ACHA6A_000484"
     CDS             19922..20584
                     /gene="deoC"
                     /locus_tag="ACHA6A_000484"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8968718.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20605..21003
                     /locus_tag="ACHA6A_000485"
     CDS             20605..21003
                     /locus_tag="ACHA6A_000485"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8968719.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21069..22151
                     /locus_tag="ACHA6A_000486"
     CDS             21069..22151
                     /locus_tag="ACHA6A_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8968720.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22363..23925
                     /locus_tag="ACHA6A_000487"
     CDS             22363..23925
                     /locus_tag="ACHA6A_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968721.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            23918..25051
                     /locus_tag="ACHA6A_000488"
     CDS             23918..25051
                     /locus_tag="ACHA6A_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8968722.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25051..26007
                     /locus_tag="ACHA6A_000489"
     CDS             25051..26007
                     /locus_tag="ACHA6A_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8968723.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26106..27272
                     /gene="deoB"
                     /locus_tag="ACHA6A_000490"
     CDS             26106..27272
                     /gene="deoB"
                     /locus_tag="ACHA6A_000490"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8968724.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27296..28114
                     /locus_tag="ACHA6A_000491"
     CDS             27296..28114
                     /locus_tag="ACHA6A_000491"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8968725.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28131..28838
                     /gene="deoD"
                     /locus_tag="ACHA6A_000492"
     CDS             28131..28838
                     /gene="deoD"
                     /locus_tag="ACHA6A_000492"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8968726.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29136..29822)
                     /gene="gpmA"
                     /locus_tag="ACHA6A_000493"
     CDS             complement(29136..29822)
                     /gene="gpmA"
                     /locus_tag="ACHA6A_000493"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8968727.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(29994..30575)
                     /locus_tag="ACHA6A_000494"
     CDS             complement(29994..30575)
                     /locus_tag="ACHA6A_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8968728.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30708..31391)
                     /gene="rpiA"
                     /locus_tag="ACHA6A_000495"
     CDS             complement(30708..31391)
                     /gene="rpiA"
                     /locus_tag="ACHA6A_000495"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8968729.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            31710..32123
                     /gene="rpsL"
                     /locus_tag="ACHA6A_000496"
     CDS             31710..32123
                     /gene="rpsL"
                     /locus_tag="ACHA6A_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8968730.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32207..32677
                     /gene="rpsG"
                     /locus_tag="ACHA6A_000497"
     CDS             32207..32677
                     /gene="rpsG"
                     /locus_tag="ACHA6A_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8968731.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            32771..34858
                     /gene="fusA"
                     /locus_tag="ACHA6A_000498"
     CDS             32771..34858
                     /gene="fusA"
                     /locus_tag="ACHA6A_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8968732.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35030..36217
                     /gene="tuf"
                     /locus_tag="ACHA6A_000499"
     CDS             35030..36217
                     /gene="tuf"
                     /locus_tag="ACHA6A_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8968733.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36624..36932
                     /gene="rpsJ"
                     /locus_tag="ACHA6A_000500"
     CDS             36624..36932
                     /gene="rpsJ"
                     /locus_tag="ACHA6A_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8968734.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            36963..37592
                     /gene="rplC"
                     /locus_tag="ACHA6A_000501"
     CDS             36963..37592
                     /gene="rplC"
                     /locus_tag="ACHA6A_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8968735.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37620..38243
                     /gene="rplD"
                     /locus_tag="ACHA6A_000502"
     CDS             37620..38243
                     /gene="rplD"
                     /locus_tag="ACHA6A_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8968736.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38243..38533
                     /gene="rplW"
                     /locus_tag="ACHA6A_000503"
     CDS             38243..38533
                     /gene="rplW"
                     /locus_tag="ACHA6A_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8968737.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38572..39405
                     /gene="rplB"
                     /locus_tag="ACHA6A_000504"
     CDS             38572..39405
                     /gene="rplB"
                     /locus_tag="ACHA6A_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8968738.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39448..39726
                     /gene="rpsS"
                     /locus_tag="ACHA6A_000505"
     CDS             39448..39726
                     /gene="rpsS"
                     /locus_tag="ACHA6A_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8968739.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            39749..40096
                     /gene="rplV"
                     /locus_tag="ACHA6A_000506"
     CDS             39749..40096
                     /gene="rplV"
                     /locus_tag="ACHA6A_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8968740.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40110..40766
                     /gene="rpsC"
                     /locus_tag="ACHA6A_000507"
     CDS             40110..40766
                     /gene="rpsC"
                     /locus_tag="ACHA6A_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8968741.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            40769..41203
                     /gene="rplP"
                     /locus_tag="ACHA6A_000508"
     CDS             40769..41203
                     /gene="rplP"
                     /locus_tag="ACHA6A_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8968742.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41193..41381
                     /gene="rpmC"
                     /locus_tag="ACHA6A_000509"
     CDS             41193..41381
                     /gene="rpmC"
                     /locus_tag="ACHA6A_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8968743.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41406..41672
                     /gene="rpsQ"
                     /locus_tag="ACHA6A_000510"
     CDS             41406..41672
                     /gene="rpsQ"
                     /locus_tag="ACHA6A_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8968744.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            41730..42098
                     /gene="rplN"
                     /locus_tag="ACHA6A_000511"
     CDS             41730..42098
                     /gene="rplN"
                     /locus_tag="ACHA6A_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8968745.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42135..42443
                     /gene="rplX"
                     /locus_tag="ACHA6A_000512"
     CDS             42135..42443
                     /gene="rplX"
                     /locus_tag="ACHA6A_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8968746.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42471..43010
                     /gene="rplE"
                     /locus_tag="ACHA6A_000513"
     CDS             42471..43010
                     /gene="rplE"
                     /locus_tag="ACHA6A_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8968747.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43029..43214
                     /locus_tag="ACHA6A_000514"
     CDS             43029..43214
                     /locus_tag="ACHA6A_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8968748.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43250..43648
                     /gene="rpsH"
                     /locus_tag="ACHA6A_000515"
     CDS             43250..43648
                     /gene="rpsH"
                     /locus_tag="ACHA6A_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8968749.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43680..44216
                     /gene="rplF"
                     /locus_tag="ACHA6A_000516"
     CDS             43680..44216
                     /gene="rplF"
                     /locus_tag="ACHA6A_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8968750.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44377..44733
                     /gene="rplR"
                     /locus_tag="ACHA6A_000517"
     CDS             44377..44733
                     /gene="rplR"
                     /locus_tag="ACHA6A_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8968751.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            44754..45254
                     /gene="rpsE"
                     /locus_tag="ACHA6A_000518"
     CDS             44754..45254
                     /gene="rpsE"
                     /locus_tag="ACHA6A_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8968752.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45269..45448
                     /gene="rpmD"
                     /locus_tag="ACHA6A_000519"
     CDS             45269..45448
                     /gene="rpmD"
                     /locus_tag="ACHA6A_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8968753.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45492..45932
                     /gene="rplO"
                     /locus_tag="ACHA6A_000520"
     CDS             45492..45932
                     /gene="rplO"
                     /locus_tag="ACHA6A_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8968754.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            45932..47227
                     /gene="secY"
                     /locus_tag="ACHA6A_000521"
     CDS             45932..47227
                     /gene="secY"
                     /locus_tag="ACHA6A_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8968755.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47289..47936
                     /locus_tag="ACHA6A_000522"
     CDS             47289..47936
                     /locus_tag="ACHA6A_000522"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8968756.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48127..48345
                     /gene="infA"
                     /locus_tag="ACHA6A_000523"
     CDS             48127..48345
                     /gene="infA"
                     /locus_tag="ACHA6A_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8968757.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48378..48494
                     /gene="rpmJ"
                     /locus_tag="ACHA6A_000524"
     CDS             48378..48494
                     /gene="rpmJ"
                     /locus_tag="ACHA6A_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8968758.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48512..48877
                     /gene="rpsM"
                     /locus_tag="ACHA6A_000525"
     CDS             48512..48877
                     /gene="rpsM"
                     /locus_tag="ACHA6A_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8968759.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            48905..49294
                     /gene="rpsK"
                     /locus_tag="ACHA6A_000526"
     CDS             48905..49294
                     /gene="rpsK"
                     /locus_tag="ACHA6A_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8968760.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGVRPPKRRRV"
     gene            49374..50312
                     /locus_tag="ACHA6A_000527"
     CDS             49374..50312
                     /locus_tag="ACHA6A_000527"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8968761.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50340..50720
                     /gene="rplQ"
                     /locus_tag="ACHA6A_000528"
     CDS             50340..50720
                     /gene="rplQ"
                     /locus_tag="ACHA6A_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8968762.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            50992..51831
                     /locus_tag="ACHA6A_000529"
     CDS             50992..51831
                     /locus_tag="ACHA6A_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8968763.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            51807..52676
                     /locus_tag="ACHA6A_000530"
     CDS             51807..52676
                     /locus_tag="ACHA6A_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8968764.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52666..53463
                     /locus_tag="ACHA6A_000531"
     CDS             52666..53463
                     /locus_tag="ACHA6A_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8968765.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            53666..54412
                     /gene="truA"
                     /locus_tag="ACHA6A_000532"
     CDS             53666..54412
                     /gene="truA"
                     /locus_tag="ACHA6A_000532"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8968766.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55076..55270
                     /locus_tag="ACHA6A_000533"
                     /pseudo
     CDS             55076..55270
                     /locus_tag="ACHA6A_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55458..56234)
                     /locus_tag="ACHA6A_000534"
     CDS             complement(55458..56234)
                     /locus_tag="ACHA6A_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8968767.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            56357..56764
                     /locus_tag="ACHA6A_000535"
     CDS             56357..56764
                     /locus_tag="ACHA6A_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFM8968768.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        18206 a        10163 c        12143 g        16450 t
ORIGIN      
        1 ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt
       61 agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga
      121 tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac
      181 agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat
      241 aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca
      301 taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat
      361 accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt
      421 tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac
      481 aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa
      541 agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag
      601 cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac
      661 ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca
      721 taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt
      781 ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg aaaagttgct
      841 atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt ctccaagttg
      901 tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac taggtaatgc
      961 tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac taggaaggag
     1021 ggatagattg ttcaagactt attttcgttt caataaaata caagataatc cttttcgtat
     1081 catgaatctg atgctactta tctttattgt attatggtta ggttacataa atcatgattt
     1141 tttgatttca agtttttcat cattagggat ctttactttt ttctattatc aaaacatacc
     1201 gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg ttgctttcgt
     1261 tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag ctttggttgc
     1321 tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg gcttgttttt
     1381 tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat taccgatcgt
     1441 ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt
     1501 actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa gatttcacga
     1561 agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc tttccgttta
     1621 tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat gtgcatcgat
     1681 tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc tgattggatc
     1741 gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc agttgcagac
     1801 catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta gaaattatgc
     1861 agttgctaat atttttctta atccgatggc gttgatgcta tctacactta ttcgtggagc
     1921 atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa gtttcatcgg
     1981 tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg ttgttcgtca
     2041 aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag atttttgtca
     2101 caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa attcacacaa
     2161 aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa attttgttta
     2221 tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga
     2281 tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat
     2341 accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg
     2401 gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc
     2461 ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg
     2521 agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag
     2581 acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc
     2641 aaagttggaa aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa
     2701 tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg
     2761 accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg
     2821 agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt
     2881 gcctttctct tcatattcct tccgatgcag atttatctca gaataagata agagatagtt
     2941 acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt
     3001 acacagatac ttggttacta tcacccaatc ttactaaata gttgaaaaaa gagtcaaaaa
     3061 tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag
     3121 tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt
     3181 tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta
     3241 ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa
     3301 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
     3361 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
     3421 cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac
     3481 gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa gagggccttt
     3541 tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta
     3601 gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca
     3661 caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg
     3721 gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat
     3781 atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa
     3841 aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc
     3901 aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca
     3961 tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat tccagttata
     4021 tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt
     4081 tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac
     4141 ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg
     4201 ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac
     4261 cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc agctcactcc
     4321 atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa
     4381 ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta ctattgctag
     4441 aataacatca aggaataata agttttggtt ttcagataat agtcgataag ttgtgtattg
     4501 gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc actattacaa
     4561 gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa atgaatagtt
     4621 gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt caatttgtga
     4681 ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat tgtagtgttt
     4741 attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa
     4801 catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg
     4861 gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat
     4921 atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta
     4981 gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt
     5041 gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata
     5101 ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa aatttactag
     5161 atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag
     5221 ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg
     5281 aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa
     5341 ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta
     5401 gaagagtttt tggattatgt tagtatgcaa cagtaaggga gttcttatta tgaaaaattt
     5461 tgacacatat tatgttattg ttttttcgat atctttgctg ttaatattac taactttaat
     5521 gcagtataaa gagtatccag agtattttat tttaactttt gtatatttag taagtagcta
     5581 cacacttcta tataaattca gaagttcttc tagagtttct aaagtttgtt tcttaattag
     5641 cttattgatt ctataatatt tttgatttat ataggaaatt aaaaatatta tagaatcaac
     5701 actcagagaa cgggttggtt tttaatagat ttaataagta ctgataatga aatttctcct
     5761 ggttgggaaa acgaagttgg ttaccctgta gaagaatttt ttcaagatta ctttgcggag
     5821 tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt catcaatcaa
     5881 tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc cattatttct
     5941 tcagggatta gtgactatat tgatatgagt ggtgaaatca attatggcga taaagacaaa
     6001 attgtacgca tgataaaagc ttttatcaaa acttttgatg tgaaggcaga tcttaagttt
     6061 aatgatactt acctagtagg aaaattaatt aatatccttg ggacagagaa agatacggaa
     6121 acgtttattt cctctttgtc tatgttgttt acagataagc atttttcaac tcagcttggt
     6181 ggagaggaac tagtggaaat cttttaacca ttacgcccag cattaataaa agtatatcaa
     6241 aaaagtactc ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa cactattttt
     6301 aatgttaatt atagatagag atgaacgcgt catatggtta aaagaatgaa aaagtcaaag
     6361 atacactgct atatttcact aaggatatag cagtgtatct ttcatgtcgt ttcactggca
     6421 gttttttata aaaggaggtg tgcatttcag ttgcttttct ttggtagtta gttgaatcgt
     6481 ttaagaatta acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa aatctttgat
     6541 ggttccatct ttatttagct gtgcctgttc aaatactgtt ttgtttttac cgaatggatc
     6601 aattgcaata attgttccga tccccgcacg tttggcagca agtaatcctg agtaagcatc
     6661 ttcgataaca agacaatcag ccggagctaa gctcagtttg tctgccgctt taagaaaaat
     6721 atccggttgc ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt cccagtcaaa
     6781 ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga cagtagccgt
     6841 ggcaatcgtt aatggaatct gttggtcagt cagctggtca agagtcgctt caagcccttt
     6901 tgtcaactga agctcgtttt cattttgcaa gcaaagcata cgataatagc tttctttttc
     6961 ttctgacaat acggcgatct cagaggcggt caaatgctct gaaatgaaat gtttcaaaat
     7021 ctccgtattc gtccgaccat gaatattttt taaaatatcc tcgtctgata aaccatcttt
     7081 tgtatgatca tgtatcatat gcagccaagc agcttcatgc agatgagagt ccaaaaacat
     7141 tgttccgttg aaatcaaata taatcccttt catagttacg ctcctatagt ggtattttcg
     7201 gttgaatcag caatttgcag atcgacatat tgtgcatagt gctcttgaac ctcccgcgag
     7261 atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac ataaaaatca
     7321 tctcgattga attttttatt atcaacgagt agatatttct tacgggaatt atttagagcg
     7381 atctgctgtg cttctccttc ttcggtactg taagtagaga tcgcttcgtt gtgaacgcca
     7441 tttgaactga taaatgcttt tgtaaacttt agtcttctta aattgtcgtt ggtgatcggt
     7501 ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa tcagtagaat ttctggtcca
     7561 tctgcttgag caagaatatc aaaaactgga taattatttg taatgatccg aattttcttg
     7621 ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa aatcgtttct
     7681 ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt ttttttcttg aatctgcaga
     7741 gtggcttttt cagtgtgaga aagttcttga tctaaagtgt aggaaagact ttgagcccct
     7801 ccatggacac gcagcagctt gcccgatttt tctaattcat ctaaatctct gcgaacggtc
     7861 atatcagaaa catctaattg attgatgatt tcattaacgg taaggattcc ttttttattg
     7921 accatttcta cgatagtaag caatctttct tttttcaaca ttgtcttcac ctctaattta
     7981 aggataatct atcatataaa cggacaacag tcaatctaat tcgaacaaaa gtaaacattt
     8041 agggtggttt ttatgtttgt tacaatagat tttattgctg ttttctggtt tttatgatta
     8101 tttgtgtttg tttttgttga caaatgtttt ggatgcgttt acaatgcaat cagttagaaa
     8161 gagcgaaaac tgattataga tttgaggaga gatgtacatg cttaaattac cagaagattt
     8221 tatttttggc ggagccacag cagcctatca ggttgaagga gccactaagg aaggtggcaa
     8281 aggcgctgtt gcttgggatg actttttaga ggaacaaggg cgattcagtc ccgatccggc
     8341 aagtgatttt tatcatcaat atgcaaagga cattgaattg tgtgaacgtt ttggtgtaaa
     8401 tggtctaaga ttatccattg cttggagccg catttttcct gatggagctg ggaaaccaaa
     8461 tccagaagga atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa gaaacgtaac
     8521 gccgtttgtg acgttgcatc attttgatac ccctaaacgt ttgtttgacc atggagattt
     8581 tttgaatcga gaaacaatag aagcatttgt ttcctatgcc attttttgtt ttcatgaatt
     8641 caaagaagtc aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa ccaatcaata
     8701 tttgttaggt gttttcccac caggaatcaa atatgatttt acaaagatcg tcgcctgtct
     8761 tcataatatg atggttgctc atgcccgtgt agtgaactat ttcaaagaaa acgaacttcc
     8821 gggtgaaatt ggtgtagttc actcattaga gacgaaatat gcggcaacag acgcgccgga
     8881 agataagcat gctgcctttt tagacgatgc attgtcgatc cgtttcttat tagatgctac
     8941 ctatttaggc tattattcaa ctgaaacctt gactgctctt gatgagatat gtgaagcaaa
     9001 tcaggccagc tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg
     9061 taatgactac ttagggatca atcactacca atgccatttt gtcaaagctt acgatggaga
     9121 aaatgcaatc catcataacg gtacgggtga aaaagggacc tctgtttata aagtgaaggg
     9181 aatcggagaa cgtatttata aagaaggaat tcctcgaact gattgggact ggctgattta
     9241 tccagaaggt ctatatgact tgttgctgcg aattaaaagc gattacccac attacaacaa
     9301 aatctatatc actgagaacg ggatggggta taaagatcag tttgaagatg gcattattat
     9361 ggatcagccg agaatcgact atttgagagt ctatttggaa tccttaagca aggcgataac
     9421 agccggggta aatgtgaagg ggtatttttt gtggtcgctt atggatcttt tctcatggac
     9481 aaatgggtat aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc agaagcgtta
     9541 cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa accaaaacga ttatttaata
     9601 agtgataatg gaggaagaaa agatgaataa agaggaatta caaatgcttg gttttgagat
     9661 cgttgcatat tcaggcgacg ctcgcagtac attattgaaa ttattgaaag aagtaagaca
     9721 aggaaacttt gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt taacccttgc
     9781 acataattcg caaacaaaaa ttttagcgga agaagcatct gggaaagaga tggagatggg
     9841 attcattttt atccatgggc aggatcattt aatgaccact ttattgctga gagatctgat
     9901 tcaagacttt attgtactgt acagacagaa tcaaggatag gagaagaacg ctatggatgg
     9961 aatcattaaa caaatcgaaa aagggaaacc attttttgaa aaaatctcac gtaatagtta
    10021 tttaggagcc gttcgtgacg gatttttaac agcgatgcca gctattttat tttcaagcat
    10081 tttcattttg attgctgcgg ttcctgagat ttttggttac caatggcctg aatctgtctc
    10141 cacatggcta tggaaagtct ataattattc gatgggtgtt gtagggttac tcgtttcagc
    10201 aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat
    10261 caacaccacc tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc
    10321 gattgaaggt ggattttcaa cggaatatat ggggacaaag ggactactgg cttcctttgt
    10381 cgctgcattt ttaacagtta atttatataa gttttgtgtg ctgaaagata ttaccattaa
    10441 aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc cgagatattt ttcctttctc
    10501 tttttcagta tttgcggccg ttttaattga tttaggcatt cgacaaatct tccaaatctc
    10561 atttgcagaa gcaatcatta cggtgctgca gccgctattt acagcagctg acgggtactt
    10621 aggaatcgct attatctggg gagcgatggc gctgttttgg tttgtcggtg ttcatggacc
    10681 gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca
    10741 attgttcaat tccggtgaac aagcttctaa agtactgacg gtgggtttag ggaattttgt
    10801 aggaactatg ggaggaactg gggcaacatt ggttgttcct tttatcttcc tactctttgc
    10861 taagtctaag cagttgaagg cagttgggaa agcttccttt attccagtaa gtttcgctgt
    10921 caacgaacca ttgctatttg cggcacccat gattttgaac ccttattttt ttattccatt
    10981 tttgttaaca cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg tattgggcat
    11041 gaacagcttt atgtacgttt tgccttgggc aactccagca ccgatcggtt tggttctagg
    11101 aacgggcatg ggtatcttag cttttgttct agcagcggtt ctaatcgtcg ttgacttcct
    11161 gatctatcta ccattttgca aagcatacga tgctgtctta gtagagaaag aagcacaaaa
    11221 agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt
    11281 ggcagcgagt caagttatca gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc
    11341 tggcgcaggg acaagtggga tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa
    11401 agcaccgatc agtgcaatgg cgggtgctta tggttctcat tacgaaatgc tgcaggattt
    11461 tgatatggtg gtcttagcac cgcaagtcaa ttcttactat gatgatatca aagaagacac
    11521 cgatcgctta ggaatcaagc tggttgcgac aaaaggagca gaatatatcg gattaacgag
    11581 agatcctgaa aaagctgttc agtttattct gaaccaattt caataaagga ggagccataa
    11641 tgaaaatggg aatttggttg cgtaaagctg ttttatttgc agctgtgtta ggggctttgg
    11701 gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga gcagatattt
    11761 cgatcctgtc agatatggaa aaatctggtg caaaatatta tgaagacgat atttcaaaag
    11821 atgccttaga gattttaaaa aataatggtg tcaactatgt tcgactgcgt ttgtggcagg
    11881 atccttataa tagtaatgga gaaagttatg gagcagggac aaatgatctg gaaacgacga
    11941 ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt gctgctggat tttcactaca
    12001 gtgatttttg ggttgatcct gggaaacaga atcttcctaa agcatggcaa ggtctgacct
    12061 ttgaagaaat gaatacggca ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc
    12121 agctggacgt ttatcctgat atggttcaga tcggcaatga actgaatagc ggaatgctct
    12181 ggccttatgg caagtcttgg ggagaaggcg ggggagaatt tgatcgattg gctgcttttt
    12241 tgaagtctgg tatccaagct gttcgagaca ctcaacctaa aacaactcca gtaatgctcc
    12301 acttagctga tggtggcgat acaggagcat ttacttggtg gtttgatgaa atcacgagtc
    12361 gaggtgtgtc atttgattta attggagtgt cgtattatcc gtattggcac ggttcaatga
    12421 atgaacttca aaccaatatg aatgcgatca gccaacggta tggtaaagaa gtgatcgtag
    12481 ttgaaacagc ttatgggcat acaacagcga atgccgatac tatgccaaat gcttttggtg
    12541 aagccgaagc agctgcgggg ggatatgaac caacaccgac aggacaagca gagtatttgc
    12601 tggatcttgc cgatcggatt caggcagtac cgaataatcg cggggcgggc ttcttctatt
    12661 gggaacctct ttggtacaac ggaaatgtga gttgggcaac acaagcaggt atgaattatc
    12721 tcagtgttca atccactatg ggaaacgaat gggacaacca agcgatgttt gattttgctg
    12781 gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata aatctagtta
    12841 aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg caaacctggt
    12901 ttcaacaagg aaccgctaag ggtacgatca aaacggaagc gggcactttt tttggggact
    12961 acaaactaac tttttggcag gatcaagcat acgaggcatc agcttatcaa aatgttagtg
    13021 gcataagcaa aggaacttac acattgagtg cctatgtaat gtctggggga gagtttgata
    13081 ctaatcagtt atatatcaaa aattatggtg gagcggaact aaatactgcg gttgttaaaa
    13141 caagtgaatg gacaaaaatt acaatcaaag acattccaat tacaacaggt gtttgtgaaa
    13201 ttggggttta tagcaaggct tcaggaaatg cttggacgaa cgttgatttt gtttcattgg
    13261 tgaaacaata gactgcggat cgtttcgatt caagatcgga ggttcccttc agccacatcg
    13321 ttagcgtaga aggcaggaat cgaacctgca cctcgccttt gccaaaaata accagaaacg
    13381 atttccagtc atttttacca aaacgagtaa agcaccagac ttcatctaca ctttgtatta
    13441 tgctaaagag agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca
    13501 gataacataa agtttgttta agaaagggta accttagaat tccgtcgtga atatgatttg
    13561 agcacttaga tggatagaaa aaagttctcg gaaaacagtt cgtttttcga gaacttcttt
    13621 ttatgataag aatgaaacaa attaacttat attatagtct cttttggtag ggaaccatct
    13681 tatttttaat gaaaatcaag cattttagct attgaagaat gttgatttat agggattccc
    13741 cctccatatt atgttggctc tttattccca ctccactgtt gctggtggtt tagaagtgat
    13801 gtcatacacg atacggttga cgtgggctac ttcgtttacg atacgtacag agattttttg
    13861 caagacttcc catgggatac gggcaaagtc agcggtcatt ccgtcgattg aagttacagc
    13921 acggataccg actgtgtagt cgtaagtacg tccgtcaccc ataacaccga cagagcggat
    13981 ccctggaaga acagtgaaat attgccagat atcacggtca agtccagcag ctgcgatttc
    14041 ttcacgcaag atagcatcag attcgcgtac gatttctagc ttgtcttcag tgatttcgcc
    14101 aagaacacga ataccaagac caggaccagg gaatggctga cgccaaacga ttgaatcagg
    14161 catgcctaac tgagtcccta attcacggac ttcgtctttg aataatgtgt ttaatggttc
    14221 gatcaattca aattgcatat cttctggcag tccacctaca ttatggtgag atttgatcgt
    14281 ctgtgctgtt tctgtaccag attcgataac gtcagtatac aacgttcctt gtgctaggaa
    14341 agagacgcct tcttcaccag caagttttgt tgcttcgtca tcaaaaacat atacgaattc
    14401 attcccaatg attttacgtt tttgttctgg gtcagataca cctttcaact tgtctaagaa
    14461 gcgatctctt gcattgactt tgatgatgtt caagccgaat tttccgccta gactttccat
    14521 tacttgttcc gcttcgcctt tgcgtagcag accgtggtca acaaagatag atgtcaattg
    14581 gtcaccgatc gctttttgta aaagaacccc aacgacacta gaatcaacgc cacctgaaag
    14641 accaagcaag acttttttgt caccaacttg ttcacggatt ttttctactt gcatatcgat
    14701 aaagttatcc attgtccagt taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag
    14761 caattcgatt ccgtattcgg aatgacgtac ttctgcatgg aattggatac cataaaaatt
    14821 acgtgttgtg tcttggatag aagcaatcgg acaatctttg cttgttccga ctgtttcaaa
    14881 gccttcagga actttagtta ccaagtcacc atggctcatc catactgttt gttgttttgg
    14941 tgtttcttta aacaagacag cttgatcgct tgttacttct aacaaggctt tcccatattc
    15001 gcggttttta gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt gcataccata
    15061 gcaaatccct aatacaggaa tacctaaatt gaagatctca ggatcgatcc caaatgcatt
    15121 ctcatcatat acactgtttg gtccgccaga aagaatgatt ccctttggat tcatcgcttt
    15181 tacttcttct gcagtaatgc ggtggctcaa taattcggaa aagactccaa gatcacgaat
    15241 acgtcttgtg atcaattggt tatactgact accgtagtct aatacgataa tcttttcaac
    15301 gttcgtcata tcggcaacgt ttgtcaaatt tattacccct atccctttaa attttatctc
    15361 aaaatagtca aactatttta aaatgctaat attttcgcat gaaaatcgaa tcgatgatat
    15421 gattttcagt tttatggaga attatatcgg ctcttcctcg tgtaggcaag atatattcct
    15481 gtaaattttt caaattcaca tttttccata catcttttgc cattttaaag gcttcttcac
    15541 gatctccgat agcaaaacga taataataat tctctggatt ttggaaagcc gtatccagta
    15601 acgcaccaaa acgatccaaa taccattttt cgatcagcac gggatcggca tcgacataga
    15661 cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat
    15721 tgatcccctc aacgatcaag atatcaggct gctggatcaa ttcgtacttg tctggaatga
    15781 tatcatacac atcatgtgag taaacaggta cttttatctc ttcctgacca gattttacac
    15841 ggttcaagaa atcgatcagc atctccatat cataactttc aggaaatcct ttacggtcca
    15901 tgattccacg ctcttttaat atacgatttg gataaagaaa tccgtcagtc gtgatcagct
    15961 gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg gctgttgtac
    16021 tttttccaac ggctacactt ccggcgatcc cgataataaa aggcggtact tctacgtatt
    16081 tatgaagaaa taaaccttta ctcaagaaca aagattcaaa ttctttcata taaagataaa
    16141 tcagatgagc aagaggtaca tagatatctc gaacatcctg catggaaatg cggtcattca
    16201 aacttttgat attatctagt tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt
    16261 ctcgccattc ttctcttgcc acacggtagt agttcattgg atcattcata ttagcctcct
    16321 gcgttctccc atcaatggaa ctattttaac acaaaataaa cgcaaacccc accacttaag
    16381 agagattgcc gatctttttg tataatcttt ataatagaaa gaaacggagt cttattcttg
    16441 acttgcgcac ggatacataa aaggatagac tgttgtcatg aaagaaatga gaaggatgga
    16501 agacatgaca aatcattact attctgaacg cccagatacg gcgcacgatt ttgaacaatg
    16561 ggcttttgag ttaaaaggca gaacctttca atttgtaaca gattctggtg tattttcacg
    16621 tgaaacagtg gattatggtt cacgtgtcct gattgatgct ttcgattggg aagagttacc
    16681 tgatgaagga aagatcttag atgtgggatg cgggtatgga ccgattgggt tagctttggc
    16741 atttgcgaca cagcggtttg ttgaaatggt cgacattaat gaacgagcgg taagtctagc
    16801 gcaaggaaac gcaaagcgta accaaatcga acatgttgat atccatcaat cgaatatcta
    16861 tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc aatccgccga tccgtgctgg
    16921 aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct cggttgaaga agggcggtac
    16981 actgacaatt gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga
    17041 aacatttagt aatgcagaaa tcgtcacaaa agataaagga tattatatca tcaaaagtgt
    17101 gaaagaataa ctagctgaaa aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt
    17161 acgggtacgt atcagttctt tttttgttga gaagttttta aaaacaaaga actatttttt
    17221 cttcaaaaag aaatagtagg aacagagtcg ttgtttttta cgataaaata aataagatac
    17281 agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc aaaaaacata
    17341 ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg gaatttagat
    17401 agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg gcaggcgtat
    17461 cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt gataagacaa
    17521 gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat ttcgcttcaa
    17581 atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc actgatttct
    17641 tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc tatgtgatca
    17701 tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa gagctttccc
    17761 accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag aaatatgcat
    17821 tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc
    17881 gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt
    17941 ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga tactatcaat
    18001 tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg
    18061 ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca tcaaaaaaat
    18121 tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg gcgatcggat
    18181 gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca gtcataggat
    18241 tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg cagcagccaa
    18301 gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa
    18361 gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat
    18421 tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag gctagttagt
    18481 gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct aaaaataatg
    18541 tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc tgattgaaaa
    18601 aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac
    18661 gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt tttatcaaga
    18721 catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg gtgatgtcat
    18781 cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg gcgttggtga
    18841 cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag tggctaaaat
    18901 gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag caattcccgg
    18961 atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc
    19021 ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg ccttgcgtga
    19081 cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga gtaaaaaaat
    19141 cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg ctttcatgaa
    19201 gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga aactggctaa
    19261 ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag ctatcggaaa
    19321 tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag aagatctagt
    19381 agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag ctaagacaac
    19441 cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt
    19501 caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc cagaacgaat
    19561 tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat
    19621 cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga
    19681 agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg acactgtgac
    19741 aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt ctgtcattga
    19801 attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt tgattcatga
    19861 catcatcaca gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa ggggaaaaga
    19921 aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat ctaaagaagc
    19981 tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct gcatcaatcc
    20041 agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg tatgtacagt
    20101 aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa caacagatgc
    20161 catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa
    20221 ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa aagatcgtgc
    20281 gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca tccgagcttg
    20341 cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg
    20401 cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt
    20461 caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg aagctggtgc
    20521 tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg gtgaaggcta
    20581 ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat caacacagct
    20641 attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg cgcttgtctc
    20701 gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc atttggatta
    20761 acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga aaaaacattt
    20821 acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg tgcatgtcga
    20881 caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg agacaatggt
    20941 gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga aaaagatcta
    21001 taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt ttaggaggct
    21061 ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta gcagcagggt
    21121 tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc aaaagctcat
    21181 cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac cgttcattca
    21241 accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa
    21301 atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca aatattgacc
    21361 aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta aaaccagcga
    21421 tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat gatgtcatcg
    21481 atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct tatcttgcag
    21541 gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag
    21601 gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag
    21661 aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct gctccagata
    21721 aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc ttccatgctt
    21781 caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa tcaggcgaca
    21841 aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa tacacattaa
    21901 acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga acagtagtag
    21961 aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac actgtttatg
    22021 gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag
    22081 cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca gaaactcctg
    22141 aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag gctatggttt
    22201 gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg ataagggaac
    22261 aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg aatcttagtc
    22321 aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca agaaaatatt
    22381 gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa tgacaacatc
    22441 aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa
    22501 tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa aattttgatg
    22561 cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg gattgggatg
    22621 gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat cgtgttggga
    22681 agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa
    22741 gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga tatttcagtt
    22801 ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga tgtactgatt
    22861 tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat cgtgatcatg
    22921 aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt agatgaaatc
    22981 aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg tacagtcaac
    23041 gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac ggtttcattc
    23101 aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga aaatctagtg
    23161 gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt tcgtgctggc
    23221 gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat ccaagctttg
    23281 actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga catcactaat
    23341 aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga ccgtcataaa
    23401 tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac ctatcatcaa
    23461 aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca tgccagagaa
    23521 ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa agctttatca
    23581 ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc tgatctgttg
    23641 attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat tcataaacgt
    23701 ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga attagaagaa
    23761 attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt cggtatcgtt
    23821 gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta taccttagaa
    23881 gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt
    23941 ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag gcgcgatatt
    24001 gatggctgct tttggttttc agcccatcca aggttattct tctatgctag atgcagccct
    24061 tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga tttttacagc
    24121 attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt ctggacaagc
    24181 actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt
    24241 tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg cagcggctgt
    24301 tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa
    24361 ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg aaagctatcg
    24421 ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt taccatggtt
    24481 aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct
    24541 tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc gttctgttgg
    24601 attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga tcgttttgtc
    24661 gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat taggaacgta
    24721 tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa tggctgtttc
    24781 cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt tcagtatttt
    24841 gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt
    24901 atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc
    24961 taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat cgaaaccaac
    25021 gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat tgccttgatc
    25081 cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg cggaactttt
    25141 tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat gggtgctttt
    25201 agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac accatggatc
    25261 gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc agtagcaaca
    25321 gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat ggcacctgct
    25381 ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa catcacgcag
    25441 aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg accaattttc
    25501 tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct agcttgggtg
    25561 atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct
    25621 gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact gatttctggc
    25681 ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa cttttcagct
    25741 ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg taagtggaat
    25801 cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt gagtatcatt
    25861 ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat tgctccatat
    25921 ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc aaaagcaaac
    25981 gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg ggttacggga
    26041 agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag aaagaaggaa
    26101 taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt ggagaagcac
    26161 cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt gcgaaagaag
    26221 caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt cctcttgaag
    26281 gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa gtttctgtag
    26341 ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa actccatttc
    26401 gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc
    26461 gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat tacggtgaac
    26521 accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc cttcagatcg
    26581 cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa ttcgttcgtg
    26641 atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg tacgtgggtg
    26701 aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat ccattcggac
    26761 acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt ggaaagatca
    26821 acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc aatatggatg
    26881 gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc tttaccaatt
    26941 tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat gcgcatgcga
    27001 ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa gacgacttgc
    27061 taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat catacgcgag
    27121 aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag
    27181 gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct tcgactgaaa
    27241 atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa agcaaatgac
    27301 caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag gagtaaaaga
    27361 aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg aaatcgaaga
    27421 tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag tagttgggca
    27481 tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca tgcaagggag
    27541 attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac gtgtgatggc
    27601 cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca acgaatcatt
    27661 tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg aaaatccttt
    27721 gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc aagcttatac
    27781 gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga
    27841 aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc
    27901 tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca tcgtagcagc
    27961 tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag caggtatgca
    28021 agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac aatcttttaa
    28081 agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa atgagtgtgc
    28141 atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga gatcctttgc
    28201 gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat caggtacgcg
    28261 gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa ggaacaggaa
    28321 tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat ggtgtaaaga
    28381 aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag
    28441 tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc caagcctttc
    28501 attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata gctaaagaaa
    28561 agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac aaagatgatt
    28621 tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg gaagcagcgg
    28681 ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg accgttagcg
    28741 accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa
    28801 tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa aaagctgccc
    28861 tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg acttggctgg
    28921 acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt gaatctgccg
    28981 tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt gatgaatatt
    29041 gtgacttgtg accgagaagt tggcaggaga aaaccgttta tacgaaaaaa gggactgtga
    29101 caaatcgtgt cacagtccct tttttatagt gttttttata ggtagtattt tttagcaact
    29161 gttaagtcat catttaattc ataaacaagt ggttgacctg ttggaatttc aagatccatg
    29221 atgtcttcgt cagagatacc ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt
    29281 gcagctacta aaacagtttt gttgtctagt aatgctggag caatttcatc ttgccagaat
    29341 ggcaaagcgc gttctaatgt cactttcaag ttttctccac caggaatatc gcgtttgtct
    29401 aacattgcgt aacgacggtc atttgctgca gaaccttcgt cagtttcttc cattaatgga
    29461 ggaagagtat cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta tttttcagct
    29521 gtttcttttt tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa gcgccatgat
    29581 ttgatttgtg gtacccaaag ttgatctgaa tattctaaga tcaagttaca tgttttgatt
    29641 gcacgagtta aaacagaagt gtaagcaaca tcaaattcga ttccagcttc tttgattttg
    29701 cgtccgcctt cttttgcttc ttcgattcct tctggtgcta gatttacatc agcccaacca
    29761 gtaaattggt tcaatgcatt ccattcacta agaccgtgac gagaaaaaac taattttggc
    29821 atgtttagct ctccttttaa gataggttta tatttctgaa attttcagtt ctgttttatt
    29881 gtacaatttt ttggaagaga tttccatcga aaaattagat aagattctat gagaaaacaa
    29941 gtagagaata taaaaaaacg aacagtgccc acgcaactgt tcgtttttag gtattagatg
    30001 cataattgct cgatttcttt ttcaggtaaa ttgatatatt tataacatgt tccaactgaa
    30061 acaccgcatt ttgaagaaat aaattggatc gtttcttttt tttcataata gagacgtcgg
    30121 atttttttga tcgtttttgc atcgattttt ggtcttccgc cgatttttcc ttttttacga
    30181 gcttcatcta atccaactaa tgtacgttct ttgatcaatg cgcactccat gtttgctagg
    30241 ttttccatta attggaaata gatttttccc atttcgcgcg tatcgattcc ttcatcaaga
    30301 ctaatcaagt gcagcccgcg atctttgaat aattgcgtga gttctgttaa ttgccgcgta
    30361 gatttgccta atcgatgaag ttggcaaatg actagtgcat cgccttgttg catgtcagcg
    30421 atcactgact ccaaatgaac agcagtagaa tcattttgtt ccacttcata tgtctcatgg
    30481 aaaatttctt gacaaccaaa gtcagataat tgatcaacct gagttgttaa atcaacgtca
    30541 tttatggttg tgcgtgcata accaataatt ttcatggtga ttcctttcta gcagtgattt
    30601 tctaactgga tataacagta aaaatgtagc ataaagtacg tcaaaaagat agttttttcg
    30661 caaattttcc gctgtctttt tttattggat gatcaagcag tttttcttta tttacgacgg
    30721 attgcgtcaa gaatctcaac gcctttttct gagccgacaa ctactttaga aaccatgttt
    30781 aggaaaagcc catgttctac taccccaaca agttcatcta aataggtagc taagctttct
    30841 ggctgttcga tgacttctag atgcagatca atgatgtaat gaccaccatc tgtggttaag
    30901 acttctccgt tttcgtctgt acgcaagacg ggttggaatc ttttttcttc aaaaatatga
    30961 agtagctgtt gggagccata agggatcact tcaactggga gtgggaactt cccaagttta
    31021 tcgactaatt tagaagaatc aacgatccaa atcgtttctt ttgagtacgt agcgactatt
    31081 ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt gaaaatcagc actgatttca
    31141 tcagcaccgt ctattgtcaa atcgactacg gggacttcat caatactctt taacggtatt
    31201 cctaattttt ccgcttgttc ttttgtcgca tttgaagtag taactcctgt gatacgtaaa
    31261 ccttcttcac gcatacggcg accaatctct tctaccatat aataggcggt tgaaccggtc
    31321 ccgagaccta cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc agctaattgt
    31381 tttaaattca tctttgcgtc cacctttttt tagtttgagc atcgtgcttt cctcactatt
    31441 ttagcgtgat ttacggtaaa aagataccat tcttttttat tccaataaag ctttttcaaa
    31501 aaaaattgca tctcttgttg acatgttttt tcattcgtga tattctaaca aaggtgcttt
    31561 atctacaggt ttctgtgaag aaacgtgctg actgtttatc aaagcatatt tgataaggat
    31621 gcagacattg catttatttt ttatcacaaa aaaagaacca cctggatgtg tggaactaga
    31681 cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat taaccaatta gtacgtaaac
    31741 ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt gaacaaagga tataacagct
    31801 ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg
    31861 gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt
    31921 caaacttaat tgaagttact gcttatatcc cagggattgg tcataactta caagaacaca
    31981 gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc aggggtacgt taccatatcg
    32041 ttcgcggtgc tttagatact gctggtgtta acgaccgtaa acaaagccgt tctaaatacg
    32101 gaactaaaaa acctaaagct taattttaat tattaaacaa atgaaacttg cttctactat
    32161 atagaagtaa ttactatgaa caatatttcg gaaggaggag ttacggatgc cacgtaaagg
    32221 tcctgttgca aaacgtgatg ttttaccaga tcctatttat aactcaaaat tagtaactcg
    32281 cttgatcaac cgtgtaatgg ttgacggaaa acgcggtatt gcagctaaca ttatctataa
    32341 tgcctttgac ttgatcaaag aatctacagg taacgatcca ttagaagtgt ttgaacaagc
    32401 aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta
    32461 ccaagtacca gttgaagttc gccctgaacg ccgcgttact ctaggtcttc gttgggtagt
    32521 aaactacgct cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa aagaaatcat
    32581 ggatgctgca aacaacacag gtgcttctgt taaaaaacgt gaagacactc ataaaatggc
    32641 tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt tggtcggctt
    32701 ttataagtcg acctcagata gtgtccttat ttttagcaac tagatagtaa agagaggaga
    32761 aacaaggaga atgaccagag aattttcttt acaaaacact cgtaatatcg gtatcatggc
    32821 ccacatcgat gctggtaaaa cgactgctac tgaacgtatc ttgtactata ctggtaaaat
    32881 ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg gactggatgg aacaagaaca
    32941 agaacgtggt attacgatca cttctgctgc tactacagca caatggaagg gtcaccgaat
    33001 caacatcatc gacacaccag gacacgtaga cttcacagtt gaagttgaac gttcattgcg
    33061 tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac ctcaaactga
    33121 aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg caaacaaaat
    33181 ggacaagatc ggtgctgact tcttatattc agtatcaact ttacatgacc gtttgcaagc
    33241 aaacgctcac ccaatccaat taccaattgg tgcggaagac gacttcacag gaattatcga
    33301 ccttgtaaca atgaaggctg aaatgtatac aaacgatctt ggaacagaga tcgaagaaac
    33361 agaaattcct gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc
    33421 tgtagctgaa actgatgaag aacttacttt gaaatactta gaaggtgaag aaatcactga
    33481 agctgagtta aaagaaggta tccgccgcgc aacagttaac gtagaattct acccagtact
    33541 atgtggttca gcatttaaga acaaaggggt tcaattacta ttggacgctg ttcttgatta
    33601 cttgccatca ccgttagata tcccagctat caaagggatt gaccctaaaa ctgatgaaga
    33661 agtagaacgt cctgcagacg acagcgctcc tttctcagct ttagcattta aagttatgac
    33721 tgacccattc gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt tacaatcagg
    33781 ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta tcctacagat
    33841 gcatgccaac tctcgttcag aaatctctga agtttacgct ggagatatcg ctgctgctgt
    33901 tggtttgaaa gacacaacta ctggggacac actatgtgat gaaaagagtc tagttattct
    33961 tgaatccatg gaattcccag aaccagttat ccaagtcgct attgaaccta aatcaaaagc
    34021 tgaccaagat aaaatgggtg tggctttaca aaaactatct gaagaagatc ctactttccg
    34081 tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg aacttcactt
    34141 agatatcatc gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc
    34201 tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta agtttgtacg
    34261 tcagtctggt ggtaaaggtc aatacggtca cgtatggatt gaatttacac caaatgaaga
    34321 aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat
    34381 tccagctgtt gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc
    34441 attagtagat atcaaagcta aactttacga tggttcatac catgatgtcg attcaaatga
    34501 aactgccttc cgtgttgctg cttctatggc attacgtgca gcagctaaga aagctaatcc
    34561 agtaatcctt gaaccaatca tggctgtaga agttgttatc cctgaagatt atttaggtga
    34621 tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg
    34681 cggccaacaa gttgttcgtg cgatggttcc acttgctgaa atgtttggtt atgctacaac
    34741 tcttcgttct gctactcaag gtcgtggtac atttacaatg acatttgacc actacgaaga
    34801 tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac ggcggaaaag ctgaataatt
    34861 tcttgaaacc ggatagtcct gcaaagagat tatccaaaga gactatattt ttaatatagt
    34921 ttcgtgtaaa ataattatcg atgatatggg catgtggtgt accttgtgta catcatatac
    34981 ctatcagaca aataaaaaat atattgtatt tttaggagga cattttaaaa tggcaaaaga
    35041 aaaatttgac cgttctaaac cacacgttaa cattggtact atcggacacg ttgaccatgg
    35101 taaaactaca ttaacagctg caatcacaac tgtactatct aagaaaaacg gcggccaagc
    35161 tatggcttac gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa tcacaatctc
    35221 aacagctcac gttgagtatg aaacagatac acgccactac gctcacgttg actgcccagg
    35281 acacgcggat tacgttaaaa acatgatcac tggtgctgct caaatggacg gagctatctt
    35341 ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg
    35401 tcaagttggt gttccttaca tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga
    35461 agaattacta gaattagttg aaatggaagt tcgtgaccta ttaacagaat acgaattccc
    35521 tggtgacgat gttcctgtag ttgctggatc agctttgaaa gctctagaag gcgacgcttc
    35581 atacgaagaa aaaattcttg aattaatggc tgcagttgac gaatacatcc caactccaga
    35641 acgtgacaac gacaaaccat tcatgatgcc agttgaagac gtgttctcaa ttactggacg
    35701 tggtactgtt gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg acgaagttga
    35761 agttgttggt attgctgaag aaacttcaaa aacaacagtt actggtgttg aaatgttccg
    35821 taaattgtta gactacgctg aagctggaga caacattggt gctttactac gtggtgttgc
    35881 acgtgaagac atccaacgtg gacaagtttt agctaaacca ggtacaatca cacctcatac
    35941 aaaattctct gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc atactccatt
    36001 cttcactaac taccgtccac aattctactt ccgtacaact gacgtaacag gtgttgttga
    36061 attaccagaa ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg aagttgaatt
    36121 aatccaccca atcgctatcg aaaacggtac taaattctca atccgtgaag gcggacgtac
    36181 agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc acttaagttt taaagtaaag
    36241 caacatttat tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg ttccgagctt
    36301 ttttatttat tgcaggatca ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa
    36361 ataaaagagt cagtattttc taaatgaaac tttatgaaaa caaaatgttt tttacaagaa
    36421 aaagccttgc tttgtgagtt tatatatatt ataatgacaa aggtgcgttt actttagtga
    36481 attgcacaga cgtaatcaga atgatttcgt cggctaagaa acgagaggtt gcgacacgcc
    36541 cggacgcttt gccacgaacg agcgtgacgg aaattttcgt ggagctatgt ctacttttca
    36601 aaataggcga aggagggaac aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt
    36661 atgaacaccg tattttagat caatcagcgg ataaaatcgt ggaaactgca aaaagaactg
    36721 gagctagcgt gtctggtcca attccattac caactgaacg cagtctttac acaatcatcc
    36781 gtgcgactca taaatacaaa gattcacgcg aacaattcga aatgcgtaca cacaaacgtc
    36841 ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag ctagacttac
    36901 catctggtgt aaacatcgaa atcaaattat aaaactaaaa caaaaataat ggaggtgtac
    36961 tcatgaccaa aggaatctta gggaaaaaag tgggaatgac acaaatcttc actgaatctg
    37021 gtgaattaat tccagtaact gttgtagaag ctactccaaa cgtagtacta caagtgaaaa
    37081 caatggaaac ggatggctac gaagctattc aagttggtta ccaagatatg cgtgaagttt
    37141 tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct aagcgcttca
    37201 ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt aggaaaagaa attacagtag
    37261 atgttttcca agcaggagac attattgatg taacaggtac tacgaaaggt aaaggattcc
    37321 aaggggttat caaacgccac ggacaaagcc gcggaccaat ggctcacggt tctcgttacc
    37381 atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc
    37441 ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg
    37501 atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa
    37561 ttactatcaa atcagctgtg aaagctaaat aattgtagga agaaaggagg aactaaggaa
    37621 tgccgaatgt agcattattc aaacaagatg gaagccaaaa cggtgaaatc acacttaacg
    37681 aagaaatctt cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac
    37741 gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg
    37801 gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct atccgctcac
    37861 cacaatggcg tggaggtggc gtagtctttg gaccaacacc acgttcttac agctacaaac
    37921 ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa
    37981 acaacttggt agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac
    38041 aagttcttgc taacttatct atcgactcta aagtattggt tgttctagaa aaaggtaacg
    38101 actttgctgc tttatcagca cgtaacttac caaacgtttc tgtagtaact tctgataatg
    38161 tgagtgtgct agacgttgta tcaaatacaa aagtgttagc aacacaaact gctcttactc
    38221 aaattgagga ggtgcttgca taatgaactt actagacgta atcaaacgcc cagtgatcac
    38281 tgaaaaatca atgcttgcca tggatgacaa gaaatacact tttgaagtgg acactcgcgc
    38341 aaacaaaact ttagtaaaac aagcggtaga agctgctttt gacgttaaag tcaaaaacgt
    38401 gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag gatatacaaa
    38461 aaaacgtcgc aaagcgatcg ttacattgac tgaagattca aaagaaatcc aattattcga
    38521 agctgctgaa taagcactaa tagataactc attaaattag gagggaaaaa cgtggcgatt
    38581 aaaaagtaca aacctacctc aaatggtcgt cgtaacatga caggttctga ttttgctgaa
    38641 atcacaacat caacacctga aaaatcattg ttacaaccat tgaaaaacca tgctggacgt
    38701 aacaacaacg gccgcatcac tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc
    38761 gtgatcgact tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac gatcgaatat
    38821 gatccaaacc gttcagctaa catcgcgtta gtacactatg aagatggagt gaaagcttac
    38881 atcttagcac ctaaaggact agaagtgggt atgaaacttg tttcaggtcc agaagcagat
    38941 atcaaagtcg gaaatgctct tccattagaa aacattccag ttggtactgt tatccacaac
    39001 attgaaatga aacctggtaa aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa
    39061 gtacttggta aagaaggcaa atacgtattg atccgcttga actctggtga agttcgtatg
    39121 atcttagctg catgccgtgc tacaatcggt tctgtaggta acgaacaaca cgaattaatc
    39181 aacatcggta aagctggacg ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc
    39241 gtaatgaacc ctaatgatca cccacacggt ggtggtgaag gtaaagctcc tatcggacgt
    39301 aaagcaccag tttctccttg gggtcaacca gctcttggtt acaaaactcg taacaaaaaa
    39361 gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata aataattact ttgaccatgt
    39421 gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg accttttgtc
    39481 gatgagcatt taatgaaaaa agttgaagct caagcagggg ttgaaaagaa aaaagttatc
    39541 aaaacttggt cacgccgttc aacagttttc ccaagttttg ttggatatac catcgcagtt
    39601 tatgatggac gtaaacatgt accagtttac atccaagaag acatggtagg acacaaatta
    39661 ggtgaatttg cacctacaag aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa
    39721 cgctaatttg aggggaggat aaatccaaat ggcagaacaa atcacatcag ctaaagcaac
    39781 tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta gtaattgacc ttatcagagg
    39841 taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat ctgctggaat
    39901 cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa aataactttg acttagatgt
    39961 tgaaaacttg gtagtaagcg aagcttttgt taacgaagga cctacaatga aacgtttccg
    40021 tcctcgtgca aaaggatcag cttctccaat caacaaacgt acaagtcata tcacggtagt
    40081 cgtatcagaa aaataaggag ggataatcag tgggtcaaaa agtacatcca attggaatgc
    40141 gtgtaggcat catccgcgac tgggatgcta aatggtatgc tgaaaaagag tatgctgagt
    40201 tcttacacga agatttaaga atccgtaaat ttatttcaac taaacttgct gatgctgctg
    40261 tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta
    40321 aaccaggtat ggttattggt aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa
    40381 acaaattaac tggtaaaaga atccatatca atatcgtgga aatcaaaaaa ccagatctag
    40441 atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc
    40501 gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc
    40561 aagtatctgg tcgtctaaac ggagcagata tcgctcgttc agaaggatac tcagaaggaa
    40621 ctgttcctct tcatacttta cgtgcggaca ttgattacgc atgggaagaa gcagatacaa
    40681 catacggaaa actaggagtt aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa
    40741 aaaacactga gaaaggaggg aaataatcat gttagtacct aaacgtgtaa aacaccgtcg
    40801 tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag cattcggtga
    40861 atacggtttg caagctgttg attcacattg gatcacaaac cgccaaatcg aagctgctcg
    40921 tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta tggattaaaa ttttccctca
    40981 caaatcttat actgccaaag caattggggt acgtatgggt tctggtaaag gggcacctga
    41041 aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga
    41101 agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga aaactaagat
    41161 cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc agagaattaa
    41221 ccactgccga aatgcttgat caagaaaaac aattaaaaga agaattgttt aatcttagat
    41281 tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga
    41341 ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta attatggaag gaggccatta
    41401 agcgtatgac tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg gtttcagaca
    41461 aaatggataa aacaattacc gttgctgtcg aaacaaagaa aaaccaccct atctatggca
    41521 aacgcatgaa ctattctaag aaatacaaag tgcatgatga aaacaacaca gcaaaagttg
    41581 gcgacatcgt gagaattatg gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt
    41641 tggaagtagt cgaagaagca gttattatct aataacacga gattttccta tatagattga
    41701 atgcaaaatc tgaaaggagg atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg
    41761 ctgataactc aggcgcacgt gaaattttga cgatcaaagt ccttggtgga tctggtcgta
    41821 aaactgctaa catcggtgat gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg
    41881 ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc
    41941 gtacagacgg ttcatatatt aaatttgatg aaaatgctgc agtgattatt cgtgatgata
    42001 aaagccctcg tggaacacgt atctttgggc cagttgcacg tgaactacgt gaaaacaact
    42061 tcatgaagat cgtttcacta gcaccagaag ttttataatc ttgagacact tatcaaagga
    42121 ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt taaagttatc actggaaaag
    42181 acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa aaaagataaa gttattgtcg
    42241 aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca agctgctcct caaggtggaa
    42301 ttgttgagat ggaagcgcca attcatgttt ctaacgtgat ggtggttgac tctactggtg
    42361 tagctggccg agttggctac aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa
    42421 ccggtgaagt tttagataaa taaaagttta aggaaggagg gtcttactga atgaaccgcc
    42481 taaaagaaaa atatcttaaa gaagtaactc catcattgat ggaaaaattt aactatagct
    42541 ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg
    42601 tatcaaacgc taaaaactta gataaagcag ttgaagaatt aactttgatc actggtcaaa
    42661 aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc
    42721 caattggtgc aaaagttaca ttgcgcggag aaagaatgta cgaattctta gacaaattgg
    42781 tttctgtttc actaccacgt gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg
    42841 gtcgtggaaa ctatacttta ggtattaaag aacaattgat cttcccagaa gtagattatg
    42901 atttagtaga taaagtacgt ggtatggaca tcgttattgt aacaacagca aactctgatg
    42961 aagaatctcg tgagttgttg acacaattag gcatgccatt ccaaaaataa ttaaaggagg
    43021 cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa
    43081 cacaagctta tactcgttgc gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc
    43141 atctttgccg tatttgcttc cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga
    43201 aagctagctg gtaaaaagta aattcgcata agaaggaggt aaaagatcaa tggtcatgac
    43261 agatccaatt gcagattttc taactcgtat ccgtaacgca aacatggtta aacatgagtc
    43321 tttagaagta cctgcatcaa aaattaaacg tgacatcgct gaaatcttga aacgtgaagg
    43381 tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct
    43441 aaaatttggt aaaaacggcg aacgtgttat cacaaactta aaacgtatct ctaaaccagg
    43501 tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt taggtatcgc
    43561 aatcatttcg acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg
    43621 cggcgaagtt atcgcttacg tatggtaatc aagtagaatc tacaaggagg tgtctttaag
    43681 tgagccgtat cggaaataaa gtcgttgtta tccctgaagg agtaacagtt actcaagacg
    43741 gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac tcgtactttt tcagcagata
    43801 tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa
    43861 tgaaaacaat tcatggaaca actcgtgcga acttcaacaa catggttgta ggtgtcagcg
    43921 aaggtttcca aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa ttacaaggat
    43981 caaaacttgt attgaacgtt ggttactcac atccagtaga aatcacacca ccagctggag
    44041 taactgttga agtaccagct aacacacaag taattgttaa aggtgcgaac aaagaagaag
    44101 ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag
    44161 gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt aaataatcgt
    44221 ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca tttggctgaa gtttaccttc
    44281 aaaaaaatac atttttactt gtgccgcagc ttctattttt cgaaacgtat ttactgcttg
    44341 aaatagtagt acaataaaaa taaagaggtg acaattgtga ttacaaaacc agataagaac
    44401 aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc
    44461 ccacgcttga acgtttttcg ttctaacaaa aacatctacg ctcaaattat tgatgacgta
    44521 gcgggtgtaa cgctagcaag tgcctctacc ttggataaag aaatttcagg tggaacaaaa
    44581 acagaagcag cacaagctgt cggtaaacta gttgctgaac gtgcagctga aaaaggcatt
    44641 aaagtagtag tctttgaccg tggtggatac ctttaccatg gccgtgtgca agctttagct
    44701 gaagctgctc gcgaaaatgg actagaattt tagaagaagg aggaatacca ttcatggttt
    44761 atatcgatcc aaaacatttg gaattagaag accgcgttgt tgcaattaac cgtgtaacaa
    44821 aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa
    44881 acggacacgt agggtttggt acaggtaaag ctcaagaagt tcctgaagca atccgtaaag
    44941 caattgaaga tgcgaagaaa aacttagttg aagtacctat ggttggttca acaatcccac
    45001 acgaagtaat cggtgctttt agtggtggta gaatcctaat gaaacctgct gtagaaggtt
    45061 ctggggttgc tgctggtgga ccagttcgtg ccgtattaga attagctggg gtagcagata
    45121 tcacttctaa atctttaggc tcaaatacac caatcaacgt tgttcgcgca acagttgaag
    45181 gtttgaaaca attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag
    45241 aattaatcgg ataaggagga caacaataat ggctgaatta aaagttactt taaaacgcag
    45301 tattatcgga cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc ttacaaagat
    45361 taacagttca gtggttaagc cttctaatga agcaattaaa ggcatgatca atactgtttc
    45421 tcacctagtg gacgttgaag aaatttaatt tagaaaaatc ataactagaa cattaaggag
    45481 gtgccaaacc tatgaaactt catgaattaa aacctgctga aggttcacgc caagtacgta
    45541 accgtgttgg tcgtggtact tcatctggta acggaaaaac tgccggacgc ggacaaaaag
    45601 gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga caaacaccat
    45661 tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac tatgcagtca
    45721 ttaatcttga tgtattgaac cgctttgaag acggagctga agtaactcct gttgctttag
    45781 ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct aatggggaac
    45841 taaacaagaa attaactgtg aaagcagcaa aattctctaa agcagctcaa gaagcaattg
    45901 aagcggctgg tggatcaatc gaggtgatct aatgtttaga ctattaaaag atgcttttaa
    45961 agtaaaagac attagatcga aaatcctatt tactgtattt gtgttgtttg tatttcgcct
    46021 aggtgcacat attactgtac caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt
    46081 gccatttttc aacatgttga acttggtaag tggtagtgcg atgcaaaact tttccatttt
    46141 ctcaatgggg gtttcaccat acattactgc ttctatcgtc attcagcttc ttcaaatgga
    46201 catcgtgcct cgatttgttg aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa
    46261 tcaagcgact cgatatctga cattagtatt ggcatttatc caatctgtcg gtatcactgc
    46321 tggattcaat caatacgctc aattaggttt cgtgaataat cctaacgtag caacttacgt
    46381 aacgatcggt ttgattctaa cagccggtac gatgtttgtt acttggctag gagaacagat
    46441 cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt tttgcaggta tcatttctag
    46501 attgcctgaa agtatcagag aaatttatga agattacttt ataaatattg atccttcaga
    46561 catatggaaa tctgctattt ttgtggccat tttactagta gcggtcttgt tgatcgtgac
    46621 attcgtaaca tttttccaac aagctgaacg caagatcccg atccaatata cgaaacgcgt
    46681 agctggcgcg cctacaagca gctatctgcc gttgaaagta aatgcagctg gagttattcc
    46741 agttatcttt gctagttcgt ttattgctac gccaaatgct gttttacaag cattgagcgg
    46801 tagattcggt tctgaaaact ggtacaaagt gatgatggag atctttaact ataatacagc
    46861 acctggtgct acgatttata cgattctgat cgttgccttc actttcttct acgcctttgt
    46921 tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt atataccaag
    46981 cgtacgccct ggtaagggga ctgaagagta tgtctcaaga ttactgatgc gtttaagtac
    47041 agttggtgca gtcttccttg gtttagtggc attgcttcca atcattgctc aaatggtttg
    47101 ggatcttcca caatcaatcg gattaggcgg tacaagctta ctgatcgtta tcggtgtagc
    47161 tttagaaact gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg ttggattcat
    47221 tgagtaagaa agcgtgttga ggtttcccct caacacacgt tctcacacat ttattaggag
    47281 ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc aggtaaagga acacaagctg
    47341 aaaaaatcat tgatgcttat ggtattccgc acatctcaac aggagatatg ttccgcgcag
    47401 cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc ttatatggat aaaggtgcat
    47461 tagttcctga tgaagtaaca aatggaatcg tgaaagagcg actagctgaa ccagatacag
    47521 agaaaggctt cttattagat ggctttccac gtacgctaga tcaagcaaaa gctctggacg
    47581 caatgctaaa agatttgaat aaaaaaattg atgctgtcat cgatatccat gttggtgaag
    47641 aaatcttggt cgaacgtcta gccggccgat ttatctgcag caattgtgga gcaacttacc
    47701 ataaaatttt caaccctaca aaagttgaag acacatgtga tcgttgcggt gggcatgaat
    47761 tctatcaaag agaagatgat aaacctgaga cggttaaaaa tcgtctggct atcaacatca
    47821 aaaatagtga accaatcttg gcttattata aagaacaagg tttgctaaac actattgatg
    47881 gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa aatcattgaa aaatagcacg
    47941 attgcccgta gccgacttgc attatgcagt tactcttgtc aagttcaata aattatgata
    48001 gaatatgtca gtccgacttc tagagaatcc tctagatgat ccattttgag aattttgagg
    48061 tgggaactgc agtttccgcc attttatcgt gtgaaaatgc gaaacaagta caaggaggta
    48121 ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga aactttgccg
    48181 aatgcaatgt ttaaagttga actagaaaac ggacaccaag ttcttgctac tgtttcaggt
    48241 aaaattcgta tgcattacat ccgtatctta cctggtgaca aagtaacggt tgaattgtct
    48301 ccatacgatc ttaaccgtgg ccgtattact tatcgcttta aataattgta ctccgtaaaa
    48361 ctacaggagg tattatcatg aaagtaagac catcagtaaa accaatgtgc gaacattgta
    48421 aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca aaacataaac
    48481 aacgtcaagg ataattggag gtgctataaa tatggctcgt attgcaggag tagatattcc
    48541 tcgtgataaa cgtgtagtag tttcacttac ttatatctat ggtattggta acactactgc
    48601 taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa
    48661 tgaacaaaca gatgctatcc gtgcagaagt agataaatta aaagttgaag gggatcttcg
    48721 tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc ggttcttacc gtggaatccg
    48781 tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa
    48841 aggcccagct cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa
    48901 cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg
    48961 tattgcacat atccattcta cattcaataa cactatcatc atgatcactg atactcacgg
    49021 aaatgcatta gcgtggtcat cagctggttc attaggtttc aaaggaagca aaaaatcaac
    49081 tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg aacacggatt
    49141 aaagactgtt gaagtaacag ttaaaggacc tggttctgga cgtgaagcag caattcgttc
    49201 attacaagct acaggtttag aagtgactgc aattcgtgac gtgactccag ttcctcataa
    49261 tggtgtccgc cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat
    49321 cagtaacgtc attgaacaag acacttttcg ttttgaaagg ggtaaagata agaatgattg
    49381 agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa agattatggc aagttcatcg
    49441 ttgaacctct tgaaagaggg tatgggacta ctttagggaa ttccctacgt cgtattttat
    49501 tatcttcttt gccaggtgct gccatcacta atattcaaat cgatggtgta ctgcatgaat
    49561 tctctaccat caaaggtgtt agagaagacg tcactcaaat catcttgaac attaaaggct
    49621 tagcgctgaa aatgtatggc caagaagaaa aaacccttga aatcgatatt accggacctg
    49681 ctacagtaac tgctggcgat attatcgttg atagtgatgt agaaattctt aacaaagata
    49741 tgtacatctg tagtgtctct gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg
    49801 gacgtggtta tgttcaagca gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc
    49861 cagtcgattc aatctacaca cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg
    49921 ttggacaccg tgaagatttt gataaattga cgatggagat ttggacagat ggttcaattg
    49981 aaccgttgga agcaatgagt ttgtctgcga aaatcatgac tgaacatttg gatatttttg
    50041 tgaacttaac tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa
    50101 aagaaaaaat gttggaaatg acgatcgaag agttagactt gtctgttcgc tcttacaatt
    50161 gtttgaaacg tgcaggaatc aatactgtac aagaacttac aaataaatct gagcctgaaa
    50221 tgatcaaagt ccgtaactta ggacgtaaat cattagaaga agttaaagca aaattgcacg
    50281 acttaggttt aggtttacgt aaagaagatt aaaacctttt acgaaggagg gaaaccaacg
    50341 tgagttatcg taaactagga cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa
    50401 caactgattt attaatcaac gaacgtattg tgactactga agctcgtgcg aaagaagttc
    50461 gctcaactgc agaaaaaatg atcactttag gtaaacgcgg agatttgcat gcacgtcgtc
    50521 aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg
    50581 tcattgaatc agctttacaa aaactgttca acgatattgc acctcgttac gcagaacgtc
    50641 aaggcggata cacacgcatt ttgaagacag aaccaagacg cggtgatggt gcaccaatgg
    50701 ttgtcttaga acttgtctaa aatcacacta ataacatcac tttatagatg attcttttag
    50761 agtgttatga tgttggaaga tatttttccg agtctagctc agcgctgctc ctcaagcgaa
    50821 tatcgcttgg ggagtaagca ctcatcataa agtgtttttt tataccttca attacctgtc
    50881 atgacagata tgtttgaagg cataaaaaaa cattttaagc gagaaataat tgaaaatttt
    50941 gtcttttttt gatagtgtta gtaggaactt taggatgaag aaggagtaat catggagcca
    51001 attattgaat tagaaaagat aaattataaa taccagccag atgatcttcg ccccgcattg
    51061 aaagatgtct cctttacaat tgataaaggt gagtggatcg ctattattgg acacaatggt
    51121 tcaggaaaat cgacacttgc caaaacgatc aatggattgc ttctgccaga atcagggatc
    51181 gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt ggacgatccg acagatggtt
    51241 ggaatggttt ttcaaaatcc agacaatcag tttgttggtt cgacagtaga agatgacgtt
    51301 gcttttggtc ttgaaaatca agggattccc agagaagaaa tgctagtgcg tgtgaaagat
    51361 gcacttgaga aagttagaat ggctgagttt gcttcacgcg agcctgctcg tttatcagga
    51421 gggcaaaaac aacgtgttgc cattgccggc gtggttgcct tacgacctga tatcattatt
    51481 ttagatgaag caaccagtat gctagatcca gaaggtcgtg aagaagtgat ctctacgatc
    51541 aagaagatca aagaagaaag tcaattgacc gttatatcta tcactcatga tattgatgaa
    51601 gcagctaatg ccaatcggat actagtgatg agacaaggcg aacttgtccg tgaaggtaca
    51661 ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact taggtctaga tctaccattt
    51721 cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg atgtgcccag tgaatatatg
    51781 acagaagaaa ggatggtgga ttggttatgg acatccgttt tgaacaagta gacttcactt
    51841 atcagccgaa tacaccattt gaacaaagag ccttatttga catcaatatg acgatcaaag
    51901 aaaacagtta tactgcatta gtagggcata ccggaagcgg gaaatcaacc ttacttcagc
    51961 atttgaatgc gcttgttaag ccaacaagcg gtacagtcca tattggagaa cgagatattc
    52021 agccggatac ggataataag aatttaaagc ccatccgaaa aaaagttggc atcgtctttc
    52081 agtttccaga agcacagcta ttcgaagaaa cggtagcaaa agacattgct ttcggtccta
    52141 aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca ttagaacaag
    52201 ttgggctgga tgaaagctat ttggaacgtt cgccatttga actttcagga gggcaaatgc
    52261 gtagagtagc aatcgctggt gtgcttgcca tgaggccgga agtacttgta ttggatgaac
    52321 caacggcagg acttgatccc cagggtagaa aagaaatgat ggagatgttt tggcgtcttc
    52381 ataaggaaca gcagatcact attgtattag ttacgcattt aatggatgat gtagccaatt
    52441 ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg gagccgcaag
    52501 aggtctttca aaacattgaa tggctaaaag aaaaacaact aggcgtacct actgccaccg
    52561 agtttgcaga agaattaatg gctaaaggga tgaactttgc taccttgcct ttgacagctg
    52621 aagaattagc agacgctatc gtccattatg ctggaggaaa tgcccatgat gaataaattg
    52681 atatttggtc gttatatgcc tggagattcc ttgatccatc gattggatcc ccgagctaaa
    52741 ttattagcca gtttttattt tatcggaatc atttttttag caaataattg ggaaacgtac
    52801 cttttgttgg catttttcac attactttct gtcttcttgt caaaagtaga tttgaaattt
    52861 tttatccgag gcattcgccc attgatctgg ctaatcctct tcactgtagc tttgcaggtc
    52921 ttgtttacaa gtggcggaga aacttattgg agttggggtg ttttccatat cactgaattt
    52981 ggtgtgatga acggcctctt tattttctgt cgattcgtgt tgatcatttt tatgtcgact
    53041 ctactcacgc ttactacacc gcctttggaa ttatcggatg cgattgagta cttgcttcgt
    53101 ccattgaaag ccgtacggtt cccagtccac gaaatttcat tgatgctgtc gattgctctt
    53161 cgttttgtgc ctacattgat ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc
    53221 ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga aagcagtcat tcctttgctg
    53281 attcctttat ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc tatggaagca
    53341 agaggctaca aaggtgggga aggaagaacg aaatatcgtg tattgcattg gcataataga
    53401 gacacgatgg ttatcattgc ttttgtatta ttaactgtgc tactgatctt tttgagagga
    53461 taaacgttca gtgggccgct atctattctt ttcttgctga tctgtttgca agaaaggatg
    53521 cgagcagtgt atgacaagaa atattagggt ctgtggcaga ctaaggtcac aggcctcttt
    53581 cttttatctg aaaagaaaga acgaaaaaat tttataaaaa aatcattatt ccagataacc
    53641 ttttaggaac aaaggagaga aaaacatggt acgttacaaa gcaatcattg catacgacgg
    53701 aacaaatttt aatggattcc aaaaacagcc aaatgggcgt actgttcaag aagaagtaga
    53761 aaaaacatta caaaaaatgg caaatggaaa agaaatcacg gtctttggtt cgggaaggac
    53821 agatgccgga gtacatgcga ttgggcaagt aatccatttt gattatccag aggagcgtcc
    53881 gttagaacga atgcgtttcg cgctagatac ccagtcacca gaggatattg ctgtaagaca
    53941 agtagaaatt gtttcagaag acttccacgc aagatatttg gtcaaagaaa agacgtatca
    54001 atttcgagtg gatataggga aaccacgcag tccttttcga cgccattatg caagttattt
    54061 tccttaccct cttgatttgg aaaaaattca gcgggcgctt cctgatttat tagggactca
    54121 cgatttcacc tctttttgtg catcaggaag ctcgatagag gataaagtac gtacgatcta
    54181 cgaggctaag atggaggtaa atgaaacaaa agacgaattg ttgtttacat ttaggggaaa
    54241 tggcttcttg tataagatga tacggatctt agtaggaacg cttttgaaaa ttggcaatgg
    54301 ccgattgcct gaagatagta tcccagatat tattgcgaaa aaagatcgta atgcagcagg
    54361 acctacagca catcctgaag gactttattt gtatgaagtg aaatatgaat aagaggttgt
    54421 gacagaagtg gtcagcttca agaaataaga aggaatttac gaaaattact tctcaaaatt
    54481 ttgcaaaatt tcggcttatt ttcgaaggag ctacttctgg aacaccgtga atcagaggtt
    54541 gtgaaaaagc tgccctgcat caagtaataa gaacaaccaa acaaaaatag ctcgtcatat
    54601 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    54661 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacggaaaa atagttcctc
    54721 atattttgcc gtttgttgag ctaataccga aggtcagctg attgaacacc gtttaagaaa
    54781 gaggtcgtga gcctgtcatt taactctgag tcacaagtaa caatttcaaa aaacagcttc
    54841 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    54901 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttaatgag
    54961 gacagtcgga acaggtttgt catttttata tttaaatggc acaaattttt ctaattattt
    55021 gtcatttagc gctgaaaatt tttgctattt atctaaaaaa gtgataacta cttaagatga
    55081 agcagaatat ttacgccata cggcatacaa ccgcgaaaaa tataaaaaaa agaaaacgat
    55141 tgaacgcgtt tttgcagtcg caaaggaaaa gcatggaatg cgctacatca agtatcgaga
    55201 cttggaaaac aatcaggtgt atacgctgat tgtttttgct gcaatgaatc tgaaaaagtt
    55261 gtccacttgg cagttggaac atatgtcttc atatctttta ttatacaaaa atagcaaaag
    55321 caccagtcta cactcttagt aaagaggaaa ctgatccttt tgtctacatt cttaaagtac
    55381 ttaaactaag tactttattt tactctttag gattgataca gtagcatcta tccctttatt
    55441 aataatcttt tattttttta aataaagatg ttgtctccat cttcaggcac taaaactttt
    55501 tcggaaattc ctttttcttt attgaaagac tttaattctt ctcttgacag tccccaatgg
    55561 tttacagctt ccatatgaac ggcaataatt gtggaatctg gtattgcttt atgcaattca
    55621 tataaatcgt ctttccccat aataagagat tctccaacta aaaaatgatt atctccagca
    55681 tttgctacta tgatttctgg attatactga tcaatagttt gtttaacagc ttcataccaa
    55741 actgtatctc ctgtaaagta tagtgtcttc tcagtcgaat gtttgaaaac aataccgcaa
    55801 acattaccta cataattcaa gatttctcct ctgccatgtt tgccttttgt tttaaccaat
    55861 tgtatagatt caaaaactgt gtcttctcgt agtacctcca catttttaaa accatatagt
    55921 cgaatttcat tcgcatcatt ttcatcttgc acaaataatt ttatttcttt aggtaggacc
    55981 tctttcgcta tatcgtccca atgatctaga tgtaaatgtg ttacaataac agcatcaata
    56041 ccgttgataa tatcttcgac tggcattggc aaatcaacta gaggattcat ttgatctttt
    56101 cttggtgatg gaaaaggacc tttcgaaata aatggtggaa gtgttccttt ttccgctaac
    56161 attggatcaa ttaaaaattt tttacctcca tagtctaaga tagctgttgc attacgaatg
    56221 tgatgtatat tcataaacgt tgtctccttt ttgttgatat gaaatatttt tagtataatt
    56281 caattccata caataaataa accgaacaaa aaaagtcttt tactattttt acttaattta
    56341 tgaaaggttg tttaccatgt tagaccaaac agatataaat atcattgagg aactatcaaa
    56401 aaatagtcga atcacaatga aagaacttgg agaaaaagta catttgactg gacccgctgt
    56461 ctcggcgaga gtgacaaaac tagaggaaag tggagttatt gaaaattata cagtaaaatt
    56521 aaatatggaa aagctagggt tttcgattca cgctttttta attataatta cgcaaaattt
    56581 aaatcataaa ccctatttat catttattaa aaatcatgaa aaattcttga ttagaaatta
    56641 taagattagt ggagggggct gttatctttt ggaatgtaga tttacttcaa aaaaatcaat
    56701 gaacagcttt ttggaacagc ttaatgaata tgcgaattat aaattatcta ttgttataga
    56761 ttaataataa aactagcaga tggtaaaaat actaatatgc gagacacaca ttcaaccata
    56821 actatataac ttatcaattt aaaactaaac tgatggaaac aagtaaggca aaaacattta
    56881 tattttgccc tacttatttt tttaattaaa ttcattgatg attcatattt agaaaattct
    56941 atgaaggcca aatgagtatc ct
//
LOCUS       DAYYHS010000009        55939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000009 DAYYHS010000000
VERSION     DAYYHS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.009"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..837
                     /locus_tag="ACHA6A_000536"
     CDS             85..837
                     /locus_tag="ACHA6A_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8968769.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            834..1748
                     /gene="pfkB"
                     /locus_tag="ACHA6A_000537"
     CDS             834..1748
                     /gene="pfkB"
                     /locus_tag="ACHA6A_000537"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8968770.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1766..3697
                     /locus_tag="ACHA6A_000538"
     CDS             1766..3697
                     /locus_tag="ACHA6A_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8968771.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3808..5367)
                     /locus_tag="ACHA6A_000539"
     CDS             complement(3808..5367)
                     /locus_tag="ACHA6A_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8968772.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5522..7756
                     /gene="pcrA"
                     /locus_tag="ACHA6A_000540"
     CDS             5522..7756
                     /gene="pcrA"
                     /locus_tag="ACHA6A_000540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8968773.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7773..9809
                     /gene="ligA"
                     /locus_tag="ACHA6A_000541"
     CDS             7773..9809
                     /gene="ligA"
                     /locus_tag="ACHA6A_000541"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8968774.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9915..10220
                     /gene="gatC"
                     /locus_tag="ACHA6A_000542"
     CDS             9915..10220
                     /gene="gatC"
                     /locus_tag="ACHA6A_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8968775.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10220..11692
                     /gene="gatA"
                     /locus_tag="ACHA6A_000543"
     CDS             10220..11692
                     /gene="gatA"
                     /locus_tag="ACHA6A_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8968776.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11689..13119
                     /gene="gatB"
                     /locus_tag="ACHA6A_000544"
     CDS             11689..13119
                     /gene="gatB"
                     /locus_tag="ACHA6A_000544"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8968777.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13129..14184
                     /locus_tag="ACHA6A_000545"
     CDS             13129..14184
                     /locus_tag="ACHA6A_000545"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8968778.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14295..15671
                     /gene="rlmD"
                     /locus_tag="ACHA6A_000546"
     CDS             14295..15671
                     /gene="rlmD"
                     /locus_tag="ACHA6A_000546"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8968779.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            15880..16716
                     /locus_tag="ACHA6A_000547"
     CDS             15880..16716
                     /locus_tag="ACHA6A_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8968780.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            16865..17086
                     /locus_tag="ACHA6A_000548"
     CDS             16865..17086
                     /locus_tag="ACHA6A_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8968781.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            17087..19804
                     /locus_tag="ACHA6A_000549"
     CDS             17087..19804
                     /locus_tag="ACHA6A_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM8968782.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            19797..21752
                     /locus_tag="ACHA6A_000550"
     CDS             19797..21752
                     /locus_tag="ACHA6A_000550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8968783.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            21718..22878
                     /locus_tag="ACHA6A_000551"
     CDS             21718..22878
                     /locus_tag="ACHA6A_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8968784.1"
                     /translation="MKERQRKKMANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKF
                     QEIIDFVNENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEP
                     DGEFLADSVEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHF
                     EKYKSLFDQVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGK
                     TNARMHVVYENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGY
                     IYVLKSLSTNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNV
                     NAQLLERTLHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(22929..23132)
                     /locus_tag="ACHA6A_000552"
     CDS             complement(22929..23132)
                     /locus_tag="ACHA6A_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8968785.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(23148..24980)
                     /locus_tag="ACHA6A_000553"
     CDS             complement(23148..24980)
                     /locus_tag="ACHA6A_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8968786.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(24983..25429)
                     /locus_tag="ACHA6A_000554"
     CDS             complement(24983..25429)
                     /locus_tag="ACHA6A_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM8968787.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(25422..26372)
                     /locus_tag="ACHA6A_000555"
     CDS             complement(25422..26372)
                     /locus_tag="ACHA6A_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM8968788.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            26767..27384
                     /locus_tag="ACHA6A_000556"
     CDS             26767..27384
                     /locus_tag="ACHA6A_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968789.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            27481..27687
                     /locus_tag="ACHA6A_000557"
     CDS             27481..27687
                     /locus_tag="ACHA6A_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968790.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            27692..28006
                     /locus_tag="ACHA6A_000558"
     CDS             27692..28006
                     /locus_tag="ACHA6A_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM8968791.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            28007..29149
                     /locus_tag="ACHA6A_000559"
     CDS             28007..29149
                     /locus_tag="ACHA6A_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM8968792.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            29151..29693
                     /locus_tag="ACHA6A_000560"
     CDS             29151..29693
                     /locus_tag="ACHA6A_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM8968793.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            29754..31691
                     /locus_tag="ACHA6A_000561"
     CDS             29754..31691
                     /locus_tag="ACHA6A_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFM8968794.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            31902..32672
                     /locus_tag="ACHA6A_000562"
     CDS             31902..32672
                     /locus_tag="ACHA6A_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8968795.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            32665..33009
                     /locus_tag="ACHA6A_000563"
     CDS             32665..33009
                     /locus_tag="ACHA6A_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM8968796.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            33002..35245
                     /locus_tag="ACHA6A_000564"
     CDS             33002..35245
                     /locus_tag="ACHA6A_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8968797.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            35383..35592
                     /locus_tag="ACHA6A_000565"
     CDS             35383..35592
                     /locus_tag="ACHA6A_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968798.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            35589..35873
                     /locus_tag="ACHA6A_000566"
     CDS             35589..35873
                     /locus_tag="ACHA6A_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8968799.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            35873..37225
                     /locus_tag="ACHA6A_000567"
     CDS             35873..37225
                     /locus_tag="ACHA6A_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM8968800.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            37222..37650
                     /locus_tag="ACHA6A_000568"
     CDS             37222..37650
                     /locus_tag="ACHA6A_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM8968801.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            37818..38183
                     /locus_tag="ACHA6A_000569"
     CDS             37818..38183
                     /locus_tag="ACHA6A_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8968802.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            38317..38889
                     /locus_tag="ACHA6A_000570"
     CDS             38317..38889
                     /locus_tag="ACHA6A_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8968803.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            38904..39686
                     /locus_tag="ACHA6A_000571"
     CDS             38904..39686
                     /locus_tag="ACHA6A_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM8968804.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            39686..40921
                     /locus_tag="ACHA6A_000572"
     CDS             39686..40921
                     /locus_tag="ACHA6A_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM8968805.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            40995..41372
                     /locus_tag="ACHA6A_000573"
     CDS             40995..41372
                     /locus_tag="ACHA6A_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM8968806.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            41374..41568
                     /locus_tag="ACHA6A_000574"
     CDS             41374..41568
                     /locus_tag="ACHA6A_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968807.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            41552..41728
                     /locus_tag="ACHA6A_000575"
     CDS             41552..41728
                     /locus_tag="ACHA6A_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM8968808.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            41725..41868
                     /locus_tag="ACHA6A_000576"
     CDS             41725..41868
                     /locus_tag="ACHA6A_000576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968809.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            41951..43516
                     /locus_tag="ACHA6A_000577"
     CDS             41951..43516
                     /locus_tag="ACHA6A_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8968810.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            43572..44306
                     /locus_tag="ACHA6A_000578"
     CDS             43572..44306
                     /locus_tag="ACHA6A_000578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968811.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            44372..45700
                     /locus_tag="ACHA6A_000579"
     CDS             44372..45700
                     /locus_tag="ACHA6A_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8968812.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            45600..46355
                     /locus_tag="ACHA6A_000580"
     CDS             45600..46355
                     /locus_tag="ACHA6A_000580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8968813.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            46359..47570
                     /locus_tag="ACHA6A_000581"
     CDS             46359..47570
                     /locus_tag="ACHA6A_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8968814.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            47581..47892
                     /locus_tag="ACHA6A_000582"
     CDS             47581..47892
                     /locus_tag="ACHA6A_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8968815.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            47889..48227
                     /locus_tag="ACHA6A_000583"
     CDS             47889..48227
                     /locus_tag="ACHA6A_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8968816.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            48220..48651
                     /locus_tag="ACHA6A_000584"
     CDS             48220..48651
                     /locus_tag="ACHA6A_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8968817.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            48648..48989
                     /locus_tag="ACHA6A_000585"
     CDS             48648..48989
                     /locus_tag="ACHA6A_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968818.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            48992..49582
                     /locus_tag="ACHA6A_000586"
     CDS             48992..49582
                     /locus_tag="ACHA6A_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8968819.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            49592..49972
                     /locus_tag="ACHA6A_000587"
     CDS             49592..49972
                     /locus_tag="ACHA6A_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968820.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            50020..50160
                     /locus_tag="ACHA6A_000588"
     CDS             50020..50160
                     /locus_tag="ACHA6A_000588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968821.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            50680..52740
                     /locus_tag="ACHA6A_000589"
     CDS             50680..52740
                     /locus_tag="ACHA6A_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8968822.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            52811..53212
                     /locus_tag="ACHA6A_000590"
     CDS             52811..53212
                     /locus_tag="ACHA6A_000590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968823.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            53274..53981
                     /locus_tag="ACHA6A_000591"
     CDS             53274..53981
                     /locus_tag="ACHA6A_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8968824.1"
                     /translation="MGGGQLNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEID
                     GREGDLTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIK
                     YAKASGLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVY
                     GNGTRTLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVT
                     LGTGITKVEIEPRWRYL"
     gene            53978..>55939
                     /locus_tag="ACHA6A_000592"
     CDS             53978..>55939
                     /locus_tag="ACHA6A_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8968825.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRI"
BASE COUNT        17862 a        10189 c        12682 g        15206 t
ORIGIN      
        1 ataattttta tgaacgtttt tgactgattt atattgcgag tagaaaaaaa atatgctatt
       61 atttgtattg aggtgatcgt gaaaatgctt acagaggaac gtagacaaaa aatcctagac
      121 ttgcttgaac agcagaaaat cgtgaaatca caagatcttg taaacctttt taatgcttct
      181 gaatcaacga taagacgtga tttacaagag ctggagaata gtgggtttct agaaagagtt
      241 catggcggag ctaagaaaga gcaattattg aattttgagc agaatatgag tgaaaaatca
      301 ctcaaaaacg ttcacgaaaa acagcaaatc gccaaatcgg cagctgaaac cattaatgac
      361 gaagatgtca tttatttaga cgctggctct actacgttag aaatgattcc ttttctgcaa
      421 ggaaaacaga tcacagtagt gacaaattcc gttcaacatg cggcaaattt agttgatttg
      481 gagcttgcta ccattatttt aggtggaacg ataaagctgg caactaatgc tgttttagga
      541 tctaatgcga tagaacaact aaaaaatttt cattttaata aagcatttat gggaatgaac
      601 ggtgcccatc ttgaacaagg attcacgact ccggacccag aagaggcagc tgttaaacgt
      661 ttagccatga aaaattccga agagacattt gtattgattg atcataccaa attcaaccag
      721 ttgtccttca cctctgttgc acctttggaa gcagccacga tcataaccga gcgttgtcca
      781 ttagaattcg ttaatgaatt ccgtgaaaag acaacaatta aggaggcaat acaatgattt
      841 acacagtaac acttaatcca tcaattgatt atatcgtacg cgtagatggg ttaaaactag
      901 gcgatttgaa ccgaatgaag gaagacttca agttacctgg aggaaaaggg atcaatgttt
      961 cccgtatttt gaaacggatc gattctcaat ctacagcact agggttttta ggagggttca
     1021 ctggcgaatt tatttcgaag tggcttgaaa aagaacaaat ccagtctttg ttcacaaccg
     1081 taaaagaaga tacaagaatc aatatcaaac taaaatcaga tactgaaact gaaattaacg
     1141 gtttgggccc atctatttca gatgaagaaa tcgatgagtt gaagcaagca ttaggaacag
     1201 taggacaagg agatatcgtc gtattgtcag gaagcgcgcc ggctagcctg cgaaaaggat
     1261 tttatcaaga actgattgat atcattcgag aaaaaggtgc agatttcgtg attgatacaa
     1321 caggtgatga cctaaaaaat gcacttgtta agaaaccatt gttgatcaaa ccaaacaatc
     1381 acgaattagc tgagctttac aatacaacct tccaatctgt agaagaaatc attccgtatg
     1441 gtaaaaaatt gctagctgaa ggggcacaga atgtgctgat ctcaatggct ggagacggtg
     1501 ctttgctctt cacggaacaa ggaacttata aaacgaatgt attagttcgc ccattgaaaa
     1561 attcagtagg tgcaggggat tcaatgattg ccggctttat tggggcttac agccgtacga
     1621 aagatccaat cgaagccttt aaatggggag ttgcttgcgg aagcgcaaca gctttttctg
     1681 atgacttggc agctcgcgaa ttgattgatg agttgctgcc agaagttgaa atcacaaaaa
     1741 ttgattagtt tcaaggagga taatcatgaa tatcaaggat ttaatggtca aagaagcgat
     1801 gatcatggat ttgcaagcga ctgataaaaa aggcgcaatc gatgaaatgg tccaaaaaat
     1861 gtttgatgcc ggccggattt ccgatatcga aacatataaa gaaggaattc tagcccgcga
     1921 agcacaaact tctacaggtc ttggtgatgg aatcgccatg cctcacgcga aaaacagtgc
     1981 agttaaagaa gcaacagttt tattcgcaaa aagccaaaaa ggagtagatt atgaagcctt
     2041 agacggacag ccaacttatc tgtttttcat gatcgcagct ccagaaggcg caaacgacac
     2101 acatttgcaa gcattagcag cactatctcg attgctgatt gatgcagatt tcgtaggaaa
     2161 attaaaagaa gcaaatacac cagaagaagt ccaagacttg ttccaaacag ctgaagagca
     2221 aaaagaagca gaagaaaaag cagaacaaga aacacaagct gcagaagctt ctgctcatcc
     2281 agaaagaaaa ttcgttgttg ccgttacagc ttgtccaaca gggatcgctc acacttacat
     2341 ggcagaagat gctctgaaga aaaaagcgaa agaaatgggt gtggagatca aagtcgaaac
     2401 gaacggttca gaaggaatca aaaaccgatt gactgctgaa gacatcgccc gtgcagaagg
     2461 tgtaatcgtt gcagcagaca aaaaagtaga gatgaatcgc tttgacggaa aagaactgat
     2521 caatcgtcct gtcagtgatg gtatccgtaa accagaagag ctgatcaatc tagcccttag
     2581 tggtactgca ccagtattcc atggtgatgg aaaagaaagc agttcagaag aaggaaatgc
     2641 ggatggtact atcggacaaa gaatttacaa agatttgatg aacggcgtat cacacatgtt
     2701 acctttcgtt attggtggag gaatcgctat tgcgctttct ttcatgatcg accaatttat
     2761 tggcgtgcca catgatcaat tatcaaatct agggaattac aatcaagctg ctagctggtt
     2821 caaccaaatc ggacaagctg catttggttt catgctgccg gtattagcag gatttattgc
     2881 ttcaagtatc ggtgaccgcc ctggtttgat cgttggattt gctgctggtg cattagctaa
     2941 tacaggcggt gcaggattct taggtgcact gattggtgga ttcttagctg gttatgtcat
     3001 cgtattttta cgtaaattat tcaagaactt accaaaatca ttggatggaa ttaaaacgat
     3061 cctcttttat ccagtgtttg gcttactgat tactggtttc ttgatgttgc tggtcaatgt
     3121 ccctatgaaa gcaatcaatg atgcattgaa cggtttcttg acaagtatga gtggttcgaa
     3181 tgctgcttta cttggtgcat tattagctgg aatgatggcg gcagatttag gcggtccaat
     3241 caacaaagcc gcttatgtgt ttggtacagc aactttagct acgactgttg caactggcgg
     3301 aagtgtcgtg atggcttctg ttatggctgg aggaatggta cctccattgg caattttcgt
     3361 tgctacacgt ttgtttaaga ataaattcag caaaacagat caagatgctg gtttgacgaa
     3421 catcgtgatg ggactatcgt ttgtcacgga aggatcgatt ccatttgcag cagctgaccc
     3481 gattcgtgcc attccaagtt ttattattgg ttctgcttta actggcggat tagtcggagc
     3541 tttcgggatc aaattactgg ctcctcatgg cgggattttc gttgtattct tactgagtca
     3601 cccggtcatg tatttggtgt ttattgctat cggagcagtc gtatctggtg tgatttatgg
     3661 tttattgaga aaagctcctg cagcagcaga agcatagaaa taaaaaagaa gaggctggtt
     3721 tttcccagtc tcttcttttt ttatagaatc atatagaatc aagtagtgta gatgaatagt
     3781 tgttgtaaaa tagtttttcc atgttcgtta aaatgattgc ttcaaatttg tgatagaaga
     3841 tttagcctcg atttcctgct tgattcttgg caagtttgct tcatcagtca agataaccaa
     3901 aatatctcct acattcatga tcgtatcacc gtgagtcaag atttcctgat ttcctctgcg
     3961 tatagagatt agcagcatat ttttaggcca attgaagtct cgtaccatta atccgtctaa
     4021 agaactctct gccgttactg gaaaatcaaa agttgtcctt gcacctttta gtgaggcggt
     4081 ctgagtattt ccaagtagac gttccaataa tgattcgtaa attggattac cgccgagaag
     4141 gtcattgacg atataagcaa caagagcagc caatcctaaa ggcatcagat gattaagaga
     4201 gccaaccatt tctgtcacta aaataattgc tgtcagcggt gcttttccaa tcgctgtgaa
     4261 ataaccagcc atggcaataa cgacaaaatc cttgatgtag attggatcca tcccgaaatc
     4321 aaccataatg tttccataaa agctaccgat taaagccccc aatgtcaata tcggaaggaa
     4381 aatcccccct ggaagaccac caccgtaaga aatcatggaa aaaacgaatc gaagaacaaa
     4441 caaaccgata atcataatga ttggcaagga agcatgtcca aaagctaaaa cgatttgatt
     4501 gcctccgcct agagtactag gccataaata accaattggc aaaatcagca agaaaggaac
     4561 gaacccgtga aaataagctg gtaaaggcaa tcggttgtaa aattttggca aagccagcaa
     4621 gaccttctga taaataaaac ctaagatgcc tagaagtact cctaagatca ctaaggaacc
     4681 ataatatttc aacggcaaag aaggaaggtc acctagatat aaaacaggtc tcaagccgaa
     4741 aaagtaaaga gagacgaagt tagcagtgat tgctgaagca aatgatgtca gccaaacaag
     4801 tggagaaaag gagtgatgga tttcttctaa aacaaagagt aatcctgcga taggagcatt
     4861 gaatgctgct gcaagtccgc tacttgcacc gctagaaatt aaaatctttt cttctgaaga
     4921 tggactccga aacaattgtc cagctccttg tccaatggat gcgccaagct gtatagaagg
     4981 tccttctcta cctagaaaaa ggcccgcacc aactgaaagt acaccgccaa tgaacttttt
     5041 ccataaaaca gagaaccagt tcattgataa ttccccgcga agctgtccct ctacttgtgg
     5101 aatcccgctt cccttgatat ccggttcatt tttgaccaga taacccacga aaaatgctgc
     5161 aaggatgcag caaatagcta ggccaattaa gtaaatcggc tgttcccgac tcatttcata
     5221 gatacttcga actaaatcgc ttagcttttc gatagataat cgaaacaggc taacgacgat
     5281 tcctgtcagc agtccaacaa atattccttt tagaataaaa tatatctgag tcttatctaa
     5341 tttcttgatt tttccttttt tttccattca aacgcctcgc tttcttttaa tttagcgcga
     5401 aatcaacgaa ttgacaagga aaaatccata aaaagaaaca aatgattttg aactagctag
     5461 tttaatgaaa gccaagaaaa tgctataatt aacaagattg ataaaagaaa ggatgttgtt
     5521 aatgtcatcc aaagctgaat tattaaacgg gatgaacccc cgtcagaaag aagctgttct
     5581 tcacactgac ggaccattac tgctaatggc aggtgcagga agtggaaaga cgagagtgct
     5641 gacacaccgc attgcctatt tgattgagga aaaagaagtc aatccttgga acatcctagc
     5701 gattactttt accaataagg ctgcaaaaga aatgaaagaa cgtgtcaatg cgattctcgc
     5761 atctggaggc gaagatgtct gggtatcgac ttttcactcg atgtgtgtgc gtattttaag
     5821 aagagatgtt gattttatcg ggtataaccg gaactttaca atcatcgatt cttctgaaca
     5881 gctgaccttg atgaaacgga tactaaaaga actgaacatt gatccgaaaa aatatgatcc
     5941 tcgaagtata ttaggaacaa tctctcaagc aaaaaacagc ctgcagaccc ctcaggattt
     6001 tgcaaaaatg caaggcagtt attacgaaga gattgcggca aaatgttatg ctgcttacca
     6061 aaaagagctt caatataatc aatgtatgga ctttgacgac ctgatcatga atacgattcg
     6121 attatttgaa gaacatcctg attcactcac atattatcaa aacaaattcc attatattca
     6181 tgtagatgag taccaagata caaatcacgc acagtatacg ctagtcaatt tattggcagg
     6241 tagatttagg aacctgtgcg tcgtgggaga tgcagatcaa agtatttacg gctggcgcgg
     6301 tgcagatatg cagaacatct tggattttga aaaagattat ccagatgctg cagtaatttt
     6361 gctagaacaa aactatcgct ccacgaaaaa catccttagc gcagcaaacc aagtcattga
     6421 aaacaatagc aatcgaaaac caaaaaactt gtggacagaa aacaaagaag gaaacaagat
     6481 cacttactac cgtgcagata atgaacgaga cgaaacccgt tttatcgtag atcggatgca
     6541 agaagagatt cgaagcaatc atcggaatta cggcgacttt gccatcttgt accggacgaa
     6601 cgcacaatct cgtgtaatgg aagaaactct gttgaaagca aatattcctt ataaaatggt
     6661 tggagggcac aagttctacg atcgtaaaga aatcaaagat attttagcgt atttgaatgt
     6721 gctggcaaat cctcaagatt caatcagttt tgagcgtatc gttaattcgc ccaaacgagg
     6781 catcggacca ggatctatcg aaaaactacg cagttttgct tctcttcatg aatggccact
     6841 gcttgaagca gcgcaaaatg tggatctggc aaatatcggt ggaaaagcag gacagcaatt
     6901 aggtgcattt ggagagatga tccaagaagt cactcagatg atcccatact tgacagtgac
     6961 agaattgacg aaagaggttt tggatcgtag cggatatcta gaagacttaa aaatccaaaa
     7021 tacattagaa gcacaagcac gcatcgaaaa cttggaggaa tttttaacgg tcacacaaga
     7081 attcgacaaa caattcgaac agcaaaatga agaagacgct gatgcaccag aagaaaaact
     7141 aacggtcttc ttaaatgatc tggcacttgt ttccgatatt gacaatttag aagaagatgc
     7201 ttcccaagtg acattgatga ctttgcatgc agccaaagga ttagaattcc cagttgtatt
     7261 cctgattggt ctagaagagg gggtttttcc tttatctcga gcattgatgg aagaaagcga
     7321 actggaagaa gaacgccgtc tcgcatatgt ggggatcacg cgagcagaag aagcgttgta
     7381 tttaacaaat gctttctcca gaacattata tggacggacg caatataacc gaccttctcg
     7441 ttttgtagaa gaaatcgatc aggagttact ggaaatcgaa ggcatgcgtc ctacgcctaa
     7501 aaaaacacct gtattcgcta aaaaaacagc atatagctat aaacagccag agacagcggt
     7561 tgttccctct aaaagtgcta ctggagggga aaataacagc tggaaacctg gagataaagt
     7621 caaacacaaa aaatggggtc taggaacagt cgtccgagta agcgggacgt cgaaggatct
     7681 agaactagat gtggctttcc caagtcaagg agtaaaacgt cttttggctg cctttgcgcc
     7741 aattgaaaaa gcttagtagt ggagggtaaa tgatggaaca tgaaatgacg attgacgaag
     7801 ctgctaaacg agcagaagaa cttcgtacaa gactgaacca atggtctcgc gaatactacg
     7861 tagaagataa accaacagtg gaagactatg tttatgataa agaatacgca gaattagtag
     7921 caattgagga acaatatcct gatttgatca cgtctgattc tcccactcaa cgagtaggcg
     7981 gaaaagtatt agaaggtttt gaaaaagtca ctcatgatat tccgctttac agtttgaacg
     8041 acgttttcag caaagaagag ctgatagctt ttgatcaacg tgtacaaaaa gcagtgggac
     8101 gtgtagttga ctattgctgt gaactaaaaa tagatggact ttccgtttct ttgcgttatg
     8161 aagatggaaa tttcgtgaga ggtgcgactc gcggggatgg aactgtagga gaaaacatca
     8221 ctgaaaacct aaaaactgtc cggtcggtcc caatcaaatt aaaagaacca atgaacatcg
     8281 aagtaagagg cgaatgtttc atgccaaaac ggtcatttgt ccaattgaac caagacagag
     8341 aagcagaggg aaaagatatc tttgcgaatc ctcggaacgc cgcagctggt tctttgcgtc
     8401 agttagattc taaaatcaca gcgaaaagaa atctggacac ttttttatac acagtagctg
     8461 atttcggtcc tatgcaagca aaaacccagt atgatgcgct agaagagcta gaaaaaattg
     8521 gattccacac gaatcgggaa aaacgtctat gtcattcgat tgatgaagtg tggtcataca
     8581 ttgaagaata tcatgacaaa cgtgtggatt tgccctatga aatcgatgga atcgtcatca
     8641 aagtgaatga attctcgcta caagatcaac ttggttttac tgtcaaagca ccgagatggg
     8701 cagcagctta caagtttccg ccagaagaag ttgaaacgct aatcgaaaac atcgaatgga
     8761 cagtcggtcg aacaggggta gtaacgccaa cagctatcat gacaccagtc cgagtagcag
     8821 gaacgacagt aagccgtgcc agcctgcata acggtgatta tattaagttg aaggatatcc
     8881 gcttgaaaga tacagttctg atttataaag cgggagacat catcccagaa gtttcgcaag
     8941 tcgtgttaga taaacgacct aaagacagcg aagaatacca attgcctaca cattgtccag
     9001 tttgtggaag tgaacttgtc catttggatg aagaagtagc cttacggtgc atcaatccta
     9061 aatgccctgc tcaaatgaaa gaaggtttga accactttgt ttcaagaaat gcgatgaata
     9121 tcgatgggtt ggggccgcgc gtactagaac agatgtatga taaaaaacta gtagcagatg
     9181 ttgctgatct ttacaaattg acagaagaag agttattgac gttggacaaa ataaaagaaa
     9241 aatcagcaaa taatattttg acagcaatcg acaacagcaa agataattcg gtcgagcggt
     9301 tgattttcgg gttaggaatc cgacatgtcg gagcgaaagc agcaaaaatt ttagcagaac
     9361 atttcggtga tctagaaaca ttgagtaaaa gtgactatga gtcgattatt gcgcttgata
     9421 cgatcggtga tatcattgcc gacagtgtag tcacttattt cagtaacgaa gaagtccatg
     9481 aattgatgaa tgaattaaaa caagcaggcg tcaatttcga atacaaaggg cttcgaaatg
     9541 cccaattgca agaagtcgag tctcctttta aagaaaaaac agtagtcctt actggtaaac
     9601 taacacgatt tactcgtgaa gaagcaaaag agacgatcga aaatcttggc gggaaagtga
     9661 ctggtagcgt atcgaaaaaa acggatatcg tggtagcagg agaagatgct ggcagcaagc
     9721 tgaccaaagc acaagaatta ggaatcgaag tttggacaga agatcaaatg gcagatgcat
     9781 tggctaaaag tcgctcagta gaagaatgaa aaatgagttt tttcagacaa atttcaataa
     9841 acaatttcag acacggcact cttgtgttaa aatagagagc aaagttttta actcgtttag
     9901 aaagaagggg tagtatggcg attagtgaag aacaagtgaa acacgttgcc aaattagcaa
     9961 aacttgcgtt cgcagatgaa gaacttgcaa gttttaccga ccaattaggt aaaatcatcg
    10021 atatggttga aatgctggaa gaagtagata cagaaggtgt tccatttacg tcgaatgtgg
    10081 ttgaaacagt aaacgtgatg cgacaagatc gggcgcaagc tggatggaac cgcgatgaat
    10141 tattgaaaaa tgtgccggaa acagaagacg gctttatcaa agtacccgca atcatcgata
    10201 atggggaggc tggcgcataa tgacagaatt acacaatcgt tctctagaag aactgcatca
    10261 tatgcttgta tcgaaagaaa tcactgtaca agatttgaca aaagcaacat ttgaacgaat
    10321 caaagaaaca gagcctgagg tcgaagcatt cattacattg aacgaagaaa aagcattgga
    10381 acaggcaaaa gctttagatg aaaaagggat tgatgaaagc aacgtccttg ctggtattcc
    10441 aattggtatc aaggataata tcgtaacaaa agatatcttg acaaccgctg catctaagat
    10501 cctttacaat tttgaaccaa tttatgatgc aacggtcatg gataaaatct atcaagcaga
    10561 tatgatccct gttggtaagc taaacatgga tgaatttgct atgggtggaa gtacagaaac
    10621 ttcttatttc aagaaaacaa aaaatgcttg ggacccaaca aaagtacctg gtggctcttc
    10681 tggtggttct gcagcagcag ttgcagctgg acaaattcca gtttctttag gtagtgatac
    10741 tgggggaagt atccgccaac cagcagcttt taacggcatc gtagggatga aacctactta
    10801 tggccgtgtc tcacgttttg gtttgattgc ctttgcttca tctcttgacc aaatcggacc
    10861 aatgacccgt acggtgaaag ataatgcatt agcattgaca gctatcagcg gttatgacga
    10921 aaaagacggt acttcttcag gcgtttcagt tcctgatttt gcagagggac tgacaggcga
    10981 catcaaagga atgaagattg ctttacctaa agaatactta ggcgaaggtg tggcccctgg
    11041 cgtaaaagaa gctgtattga aagcagcaga aacattccgt tcattaggtg ctgttgtgga
    11101 agaagtcagc ttgcctcatt caaaatacgg tgtggctgtt tattatatca ttgcctcatc
    11161 agaagcaagt tccaacttgc aacgattcga tggtatccgc tacggttatc gctcagaaaa
    11221 tgtgaaatca ttagatgatg tatatgtcaa ctcacgttca gaaggattcg gtgaagaagt
    11281 aaaacgccgt atcatgttag gtacattctc cttaagtgct ggttattatg acgcttactt
    11341 caaaaaagcg ggacaagtcc gtacattgat caaacgagat tttgaaaaag tctttgcaga
    11401 ttatgacttg atcatcgggc catcttcacc aacagttgca ttcggaatcg gtgagaacat
    11461 taacgatcca atcacaatgt acatgagtga catcttaacg atccctgtca acttggctgg
    11521 acttccaggt atgtctgtac cggcaggttt atcagaagga ttaccagttg gattgcagat
    11581 catcggtaat tattttgatg aaaaaacgat gtatcaagcg gcatatgcgt ttgaacaagc
    11641 aacagaattc cataaacaac aaccagctat tttaggaggg aaagacgaat gaattttgaa
    11701 acagtcattg gactagaagt ccacgtcgag ttaaaaacca actcaaaaat tttctctcca
    11761 gcgccagctc attttggtgc agaaccaaac agcaatacaa acgtgatcga ctggggttat
    11821 ccaggcgtgc tgccagtaat gaataaagct gcgctggaat tcgggatgaa agcagctttg
    11881 gcattaaact gtgaaatctc taaagatact cattttgacc ggaaaaatta cttttaccca
    11941 gataatccaa aagcatatca aatttcccaa tttgatcaac caatcggtca tgacggatgg
    12001 atcgaaatcg aagtagaagg taaaaagaaa aaaatccgga tcgaacgtgt ccatttagaa
    12061 gaagatgctg gaaaaaacat gcatggtatt ggcggttatt cttacgttga tttgaaccga
    12121 caaggcacgc ctttgatcga aatcgtttct gaagctgata tgcgttcacc agaagaagca
    12181 tacgcttatc tagaagcatt gcgatcaatt atccaattta cagaagtcag cgatgtgaaa
    12241 atggaagaag gctctatgcg ctgtgatgcc aatatttctc ttcgccctta tggacaagaa
    12301 gagttcggta cgaaagcaga attgaaaaac ttgaactctt tatctttcgt gaaaaaaggt
    12361 ttagcttttg aagaaaaacg tcaagcaaaa gttcttcttt caggaggaga aatccaacaa
    12421 gaaacacgcc gttttgacga aacaacgaat aaaacagttt tgatgcgtgt caaagaaggg
    12481 tctagtgatt atcgttactt cccagagcct gatatcccga aattcgtgat cgatgacgaa
    12541 tggattcaaa aagtaaaagc tagtttgcct gagatgccag cttctcgtcg cgaacgctat
    12601 atccgtgagt ttggacttcc agagtatgac gcaatggtat tgacacttac aaaagaaatg
    12661 tctgattttt ttgaagctgt tttggaaaat ggtgcagatg ccaagcaagc atctaactgg
    12721 ttgatgggtg aagtttctgc ttacttgaac agtgagaaac tggaattagc tgagacaaaa
    12781 ttgacacctg agaacttagc aggaatgatt cgtttgatcg ccgatggaac gatcagctca
    12841 aaaatcgcca aaaaagtctt ccgtgaattg atcacaaatg gcggagaagc ccaagaagta
    12901 gttgaaaaaa atggttgggt gcaattatct gatccgaaca aactactgcc aatcattaat
    12961 gaaattttgg ataacaacca acaatctgtc gatgatttca aaaatggaaa agatcgtgcg
    13021 gttggcttcc tagtcggtca aatcatgaaa gcaactaaag gacaagcaaa tcctggtgtc
    13081 gtgaataagc tacttaaaga tgaattagcg aaacgataga ggctgaaaat gaaaaaagca
    13141 cgcgtaattt ataaccccac ctcgggaaaa gaattattga aaaaaaattt ggcggatatc
    13201 ctacaggcat tagaagaagc aggatttgaa gccagcgcct atgcaactac accagaacca
    13261 gattcagcca agaatgaagc atgtagagtc gcagaactag gttttgatct agtagtggca
    13321 gctggtggcg atggcacaat caacgaagtg gttaatggaa tcgctccgct tgaacaccgt
    13381 cctaaaatgg ccatcatccc agctggtaca acgaacgact atgctcgagc attaaaaatt
    13441 cctagagata atattaaagc agcagcagaa gtgataaaaa aacaccaaac cgtaaaaatg
    13501 gacatcggcc tttctgataa cagttacttc atcaatatcg ctgcaggtgg ctatttgaca
    13561 gaattgactt atgaagttcc atcagaacta aagagtattt ttggttatct tgcttatctt
    13621 gcgaaaggag cagagatgct ccctcgagta aagccaatca aaatgcgctt gaagtatgat
    13681 gaaggggaat atatcggaaa tgcttcgatg tttttcctag ggctgacgaa ctctgttgga
    13741 ggattcgaac agatcgcgcc agatgccaaa ttggatgatg ggaagttttc tttaatcatt
    13801 gtgaagacag caaatatctt tgagatcttg catattgcag cactcatgct gaatggcggt
    13861 cgacatgtcg atgatccaag aataatctat accaaaacaa gcagtttgtt cgttgaaaca
    13921 gaagatgata tcaatcgacc agtaatgatc aacttagatg gtgaatacgg cggggaagca
    13981 ccgatgcatt tccaaaatct tcatcaacac attgagttct ttgccaatac cgatcagatt
    14041 cctgatgaag caatcacagg tacagatgaa gaagaattag aggttgtcag caaagaattc
    14101 gtaaaagaag tcgaacgttt aacggatgaa gatatcgacg gtgacggaaa aatagcagac
    14161 gaaacaccta atcaaaagaa ttaatcaaaa gccggtgctg aacgtagtaa ggtcagtgcc
    14221 ggttttagtt gtgtaattag ctgatttata gtagagttca gtaggattga aaaataggct
    14281 taaaggagca aaatatgaca gaacagcaaa tagtaaagaa aaacgaacga tatacagtcg
    14341 aaatcgtcga cttgagttat gaaggaatgg gggtagcaaa aatcgatggt tatccattat
    14401 ttatcgaaaa tgcactccct ggagaagaag tagaaatact agtagtaaaa gcaggcaata
    14461 agtttggata tggaaaagtc gaaacattta ttacacaatc acctgatcgt cagcctgtag
    14521 aaaacaatgc attacttcgc ataggtatcg cacctttagc ccatttgaag tatgaacaac
    14581 agcttgtctt caaacaaaaa caagtagagc atgtaatggc gaagattgct aagatgccag
    14641 aagttcctgt acttccaacg gtcggaatgg cagaaccatt cggctaccgt aataaagcac
    14701 agatccctgt tcgccgtatc gatggacagt tagccaccgg tttttacaaa aagaatagcc
    14761 atgatctagt ccctatcgaa gatttttata tccaagatcc tgtcatcgat caagcaatca
    14821 aagtagtcag agatatcttg caacggttcc aagtacgagg atataatgaa gaaaaaaatg
    14881 aaggacagct tcgtcacatc attatccgtc gtggtcatta ttcccatgaa atgatggtcg
    14941 ttttagttac tcgtaaagaa aaattcttta aatccaagga aattgctgaa gctattcatg
    15001 aagaattgcc agaagtggtt tcggtcatcc aaaatatcaa tgaagaaaaa acaaatgtga
    15061 ttttaggaga caaagaaaaa gttttatatg gcagagacta tatcgaagat cagctattag
    15121 gaaaaaccta tcgaatctca gcaaagtcat tttatcaagt aaatactcaa caagcagagt
    15181 atctgtacca aacagccatc gagtttgccg agttagataa aaaagacatt gtgatcgacg
    15241 cttattcagg aataggtacg attggcttgt ctttagcaga cagagtggat aaggtgtatg
    15301 ggatggaagt tattccagaa gcaattgaag atgcacagtt caatgcttta aataataaaa
    15361 tcgaaaatgt tcattatgaa gtcggaaaag cagaaaaagt tatgaaaaaa tggcaagaaa
    15421 aagggatcaa accatctgtg atcgtagttg accctccacg aaaaggatta gatcatcatt
    15481 ttattgaatc agcaatcgaa atgtcaccag aacgaatcgt ctatatctca tgtaatccgg
    15541 caacttttgc aagagatgct aagttatttg cagaagcagg ttatgccgtg aagaaagttc
    15601 agcctgtaga tcttttcccg caaacccatc acatcgagtg tgtaggactg cttacacgtg
    15661 tggaacgttg atatgacaag gattagagcg attcgggatg gtttccggat tgctcttttt
    15721 tcgtgttgaa atgacgattt ttgggtgagc gtagagttga tggaaatgct atggagcttt
    15781 gagttgatag gagttacacc gtagacttga taggttagat gattttgata acggttacgt
    15841 gatataatat attatcgagt tttcaaggga gacaaactta tgaagcaaat ttttatattt
    15901 tcagatacac agaaagataa tatttcttat tttgaacgcg agccagcaat cttggtttca
    15961 atgagtcgta gtgatttgcc gtatttgaag gaacaggatt tttcaaatta tccagctgtt
    16021 tatgttttga ttggtggtaa taagcgttac gtgggtcagg cagcagggca atcaatttca
    16081 cttcgtttat ctcaacactt tctcaaggaa tataaagctt gggttgaatc agttttattc
    16141 tttacaagag cagatgggaa aatgtcaaaa gcagatacag attatcttga aaaacgactt
    16201 atccaagatt tccaagaaaa atctgactat gaaatgatga attcaactgc tggtaatact
    16261 tcgtatattg ataaacttca aaaagcaaaa tcggatcagc tatatgacac agtgtttgaa
    16321 attatagatg aaattgcaaa tatcgatttg tttggtactt ccgaagacag tgaaacccaa
    16381 atttatgaat ttagttcatc tggacaatat gaaattgagt atgatgggaa aaagatatct
    16441 tcgaaatctg ctagaggttt attggttgat tttgtttctc agctatttgc ggatggaaac
    16501 tacaacaatc aactgcaaga attaattgtc gatgagacgc cgtcgttcgc tataatttta
    16561 ggacgtaaac catccatgta taacggaaga ccaaattcag ccagggtagc tgatggagta
    16621 tgggtttatg caaatttcaa caaaaagaat gtcagaatca aaattgataa actagctcgt
    16681 caattgggga ttccggttaa aataaagtgg aattaatatt tgttatctcg cctttagtaa
    16741 atctatgctg aaggctttta ttttagaaaa atagtttgcg ttcgcgaaaa tatttcatgt
    16801 ttcgtataat aatgatagct catagatgat ataattgaag ataccagtag aatagaggtg
    16861 tagtatgtca atttcttata ataaattgtg gaagttattg attgatttga atatgaacaa
    16921 aactcaactt cgtgaaatat ctggcgtcag tcccaatgta atagcaaaac tcggtaaaaa
    16981 tgagttggtt gcaatggagt cattattgaa aattgctgct gccttaaatg ttgatgttag
    17041 tgaaataatt agttcagatg ttatagatgc ggaggatgtt aaataaatgg caaaaaataa
    17101 agttttaaac atcactgaaa ttgaagatgg tgtaaaaaaa atactttcaa ataggggcga
    17161 tgatttcatc actcaatttt tgtccttgta cgatatcccc aaaacgtcga ttacacgtgc
    17221 aaaatcaaaa ttcgataatg gtgaaccatt tgtgattaag aacaaacttc attatgaaga
    17281 aattgaaggc aacgttgtca ttgcgattga tgcgattgct caatcaatca aagagcaaaa
    17341 atctaaacca cgatatatct tggtaaacga ttatttagat attgctgcaa ttgatacgaa
    17401 aactcatgaa actttgaata ttccaattga agagctaccg gctaatgctg actttttctt
    17461 agcgtggaat ggcattgaga aggctgatta tcaagctgaa aatccagctg accgaaaagc
    17521 tgctgaacgt tttgcaaaat tgtatgatgt ggtggctaaa gataatcctg atgccaacga
    17581 gcatgcattt aacttgttct tgattcgagt gctgttcttg ttgtttgcag aagatacagg
    17641 aattatgaat aagggttcct ttacaaatgt tctaaaaaca agaacggctg aagatggttc
    17701 gaattttaac gaagtaatca aagaactctt tgcaatcttg gatattaatg agttagctcg
    17761 caatggcaag gatgagtggc tacttgaatt tccatatgtt aatggaaaac tgtttggtga
    17821 gccacatgtg gatttgacct tttcaaaagt ttcaagagag ctgcttattg aagcagggga
    17881 attattgaat tggaatgaaa tcaatcctga tatccttggc gcaatgattc aatcagttgc
    17941 ttcagcagaa gaccgtcacg ttgccggtat gcattataca agcgtgccga atatcatgaa
    18001 agtcatcaag ccgttattct tagatgattt gacagagtca tttgaaactt taaagcttcg
    18061 cagtgaagaa aacgagctga aggacattac tgaaaaaaca agaaacgata ataagagaac
    18121 aatcatcaat gatctaagtg ctttgttgac gcgaatttct aatattaagt ttttggatcc
    18181 cgccagtggt tctggtaatt ttctgatcat tgcttataag gaaatccgta gacttgaaat
    18241 taagattttg caattgttgg gcgacttaca aaataatgat tccatgccac tatcagtgat
    18301 tcatcttggt aattttaatg ggattgaatt ggatgatttt gcgcatgaag tagcaagact
    18361 ttcattatgg attgctgaac atcaaatgaa taaagagctt gaagaagcaa taccaggtac
    18421 cattgctgag ttgttaccgt taaaagatgc tgggaatata gtgtgtgcca attctcttcg
    18481 aattgattgg aatgaggtgt tgcgactcaa agaaaatcat gaaatatata taatgggaaa
    18541 tccaccgtat ctgggcaaaa gtcttcaaaa tgctgaacaa cgtgaagata tggaaaaact
    18601 tgttggcgat attcgaaaat ataaattgct tgattatatt tttgggtggt tttggaaagc
    18661 taaacaatat attgcaacat ctcatggctc gttcgcattt gttactacta attcaatttg
    18721 tcaaggtgaa caagttagta tcctttggcc gtacctttta aaagatgagg taagaatttc
    18781 atttgcatat cagagcttta agtggaacaa caatgcaaag aataatgccg gtgtgactgt
    18841 tgtcattatt ggtatgacaa ataatgtgaa tattcatcct aagttatatt tagagaatgg
    18901 gtcagtaatg agtgtttcaa atattaatgc atatttagct aatggtgaaa atgtatttgt
    18961 caacgaatca agaaatagta tttcaaattt accctctttg aaatttggca gtatggcaaa
    19021 tgatggtggt aatctagttt tttcagtggc agaatactgg aatttgattc aaaaatatcc
    19081 agatattaaa catttattta agaaatattt aggatcaaca ggattaataa atggagactt
    19141 aagatatact ttatggttga ccgaggaaga tgcaactaga tacaaacaaa attctcttat
    19201 taaagaaaca ataaaaaatg taaaaagtta tagggaaaaa agcaagagaa aagaaactcg
    19261 agcactagct gagaagccat ggtcatttgg tgaagttcgt tatgatgaaa ccagtgagaa
    19321 tagtattcta attcctcgtg tgtcatcaga aaaaagagat tatgttccta tgggtatagt
    19381 tgggagcgat actataattt cagattcggc tatggcaatc tataacgctc caatgtggct
    19441 gcttggtctg ttagaatccc gaatgcatat ggtgtggctg cgctctgtcg gtgggaaatt
    19501 agaaactcgg taccgttatt cagccgggtt agtctataat acttttccga ttcaggattt
    19561 atccgcccag cgtaaaaatg aaatgactcg cgtgatgaca gaaattcttg atttacgaga
    19621 atacgaaggc ggcacgttag ccgatttgta taataaagac agtatgccag aatcattacg
    19681 taagaaacac caggaactgg atgggattgt tgatagagct tatcagcaac gcccgtttga
    19741 atctgacgaa gatcgacttg gaacattact aaaactctat caggagatga caatcaatga
    19801 ctagtaattt atttgaaatt aattttgata aaaatcaatc aaaaacaaca aatgacctcg
    19861 gtatgcgtga aatgcaagaa cgtgtttatg aaaagcgtgc gtcacagtat ttgttggtaa
    19921 aatcaccacc agcttcagga aaatcacgtg cattgatgtt tgttggcttg gataaactcc
    19981 ataatcaagg aattaaaaaa gtggttattg cagttcctga acgctcaatt ggtaaatcat
    20041 ttaaagatac tgaattatcg aagtttggtt tttattggga ttgggaagta aagccaagtg
    20101 acaacttaac tattgggggc ggtgaaaaat caaaggttca acactttgtt aaattcatga
    20161 actcgactga cccaagtgat aatatcctga ttgccacaca tgctacgttg cgatttgctt
    20221 ttgaagagtt ggacgattca ttatttgaca atacgctatt agcgattgat gagttccatc
    20281 atgtgagccg tgatgataaa tctgtgttag gtaatgcctt acgtagcatc atgtcaaact
    20341 ctaatgcgca tatcttagca atgactggtt catatttccg tggcgactct gttcagattc
    20401 tggaaccagc cgacgaagat aaatttgata aggtcactta cacttattat gagcaactgg
    20461 aaggatataa atatctgaaa tcctttgcaa tggggtatag tttctatcgt ggaaaatata
    20521 cagatgcttt ggataaagta attgatgtca acaaaaaatc aattatccat attcctaatg
    20581 ttaattctgg tgagtctact aaagataaat acgcggaagt tgatgaaata cttgatttat
    20641 tgacggacgg tggtaatatc acacagaacg aggatggtat ttatgaaatc gaacgtccag
    20701 atggtaaaaa actattagtg gccgaccttg tgaatgaaga aggacgtgaa aaagtctcag
    20761 catatctagc aagtattacg gatgacatta aagatcttga taagcttgat atcatcattg
    20821 cacttggaat ggcaaaagaa ggcttcgatt ggccatttgc ggaatatgca ctgacaatcg
    20881 gttatcgtaa ctcattaact gaagtgattc aaatcattgg tcgcgtaact cgtgacagcc
    20941 ccaataagac tcatgcacag tttacaaatc ttattgcgca gccagatgca caggatgacg
    21001 aagtattatt cgcggttaac agtattatga aggcaatttc tgcatctcta cttatggaac
    21061 aagtgttggc gccagtttac aagtttaaac caaaagatgg taagccagac aaaccaggtg
    21121 atctttcaat cagaggactg aaacctgctg atacgacgga acgtacaaag aaaatcatcg
    21181 aaaatgatat ggaagatttg aaatctagca ttttacagag ccaagatatt caaaatgcga
    21241 ttgtttcagg tgctgatgcg aagatgatta ataagacgtt gattccacgt gtcatcattg
    21301 atcgttatcc tgatttgacg aacgaagagt tagaaaccgt tcgtcaacat acagttgccg
    21361 atataaatat tgcggctgga cggacaacaa cttcagaaga tggtagccgc gaaattatta
    21421 agatggccga tcgtttcatt aacattgatg aattgaatat tgatttaatc gatcaagtga
    21481 atccgttcca acgtgcgtat gaagtgatta gtcgtgatat tgatgcaccg actcttcggt
    21541 ttattcagga ttatttgtca tcaaataagt atgattttac tgacgaacaa ttggttgcgg
    21601 cctaccaagc tgctaagaag ttccgtgcag aaaatggtcg aaatcctgat cgaaatagta
    21661 aagatgatgg tgaaaaatac ttggcctttg ctttgctacg acttgctgag ataaaacgtg
    21721 aaagagaggc agcgaaagaa gatggcgaat agtttagata gcatctttga agatgacgct
    21781 tttgatgatt taaccaagca agttgaaaaa aagaaagttg taaaacaaga cccagaagtt
    21841 gctaaatttc aggagattat agactttgtg aatgaaaacg gacgagagcc agaaaaaact
    21901 cctgaatggt ctagtgaacg cgcattgtgg gctcgtctta gcggatttcg aggaaaaccc
    21961 gaaaggtctg agaaggttaa gaagtatgac actcttggtt tattggagaa agaattcgaa
    22021 ccggatggag agtttctcgc tgattcagtt gaaatcgatg acgagttgaa tttagatgat
    22081 atcttaggct cagatgatat gattgatgat ttttcagact tgttggatgt ttcccgctat
    22141 cgtagaactg tgaacgctgc agataaaatt ggccgcagaa aacatgcaag tcattttgaa
    22201 aagtataaga gcttgttcga tcaagtgcat gcagatatcg catctggcag acgaaaaatt
    22261 atcccgtttg agcagtatga tattcatgaa aatagattct acatccaaaa cggcgtaatg
    22321 atgtatatcg tttccatttc gaaggatacc tttatcgctg ataatgggaa aacaaatgcc
    22381 agaatgcatg ttgtttatga gaatgggaca gagaataaga atctgttgtt acaatcttta
    22441 gcttcgtctc tatactcgac tgaacgtcat ggaaggatgg tgacagagcc ggtagatgag
    22501 gaaacactgg ctgaaagctt tggagaagag tttacgacag gttatatcta cgtgctgaag
    22561 tcattaagca cgaatccgga tattagcaag tggaataacc tgtataagat tggtttcact
    22621 cgaaatgatg ttgaaagtag aattgccaat gcggaaaacg acgtgaccta cttaaatgca
    22681 ccagtgaagg tcatgctttc tgctgaagta caaaatgtca acgctcagct acttgagcgc
    22741 actctccatc atgtattcca ggacaaacaa gttgtctttc aggatggaaa cttcaaaaaa
    22801 gctactgaat ggtatatggt tccattgaat gatattgaat cgaaaatcaa tgaagtaatc
    22861 gctggattac aaaaataaat atgaaaatgc actgtactca tgaatcgaat acagtgcatt
    22921 tttgatattt aatcgcgaac gatttcaaca atatccgata aatcacaatc aagtgcttca
    22981 cagatgcgaa gtaagatatc tgttgttaca ttttcgcctt tatttagttt tgagagtgtt
    23041 gctgaactga tatcagtcat ttttctgagt tgtgctttat tcagtcctct gtcgattagg
    23101 agtttccaaa gcggattgta gctaaacttc atgtaatcat tcctgttcta gttataaata
    23161 tcgtatagat atccatagcg tccattgttt ttatcttcag taaagacaat ctgatttact
    23221 tgtttatcat catagtttag tgctggcaag ttcgttgtat tctcaacgat gataagttgt
    23281 ccttcgtttt gatgatccat aaagtatttg aaaagtcctt ctttcatatt gtcatcgcca
    23341 tcttgttttt tgcgttcatc taatccaaga agaggggaat cgataatgaa cacagaaggt
    23401 gtgtacttag cgtgattata caagagatgt cgatatgcca ttaccactac tgaattgata
    23461 aacgctgtat atccatgtcc gttttcggtc cattttagat ttccattgat tttgatgtcg
    23521 aaaatatcct tggcgaaaaa tgcgttacga aattcattag gtttataatg catattacgt
    23581 aacatggagt ccagtaactc agacatttct ttgtagaatt ctgcatcaaa tttctcttta
    23641 ggtttataaa ggatacgttc gtcttctggc tcattctcga ttttttcaag atcactttta
    23701 agcttatttt gcattttttc catcgagatt agctgttgtt taatatccct gtaagtttcg
    23761 attttattga ttcgatcctt taacgtctca gctttcggtt ttagttcatc gttgattgct
    23821 gcatcaagcg atttctttct tgttttaagc tcatcaagct gttccattaa ctcttgcagt
    23881 tcaacttcgg tatcatgaga ttcttccgtc aaatctgata attgagagag caatgaatgt
    23941 aattctgacc tagcagtatc gatgtaggtt gtttggacag gtttacttag ttcgccgtta
    24001 cagaatggac agctatgtga agtatcatgt tcattatgat catttaataa tcgctcacca
    24061 tctacgatta gtgttagtcg ttcaatatcc gattgatatt gataatgtaa ctgtttgtac
    24121 ctattcaaaa gaagttgagt ttcatgaaga tactgttcct tttgataaat ctcttttatc
    24181 aagattttgc tgctggctaa cgcatcattg atttgctttt caattgtttc aagttcagcg
    24241 aacattccat cgacaatttg ttgaaaatca tcagagggag cctcaggtaa ttctgattta
    24301 gccacttcgt agttttcata taattcagaa aggcttgaat tgatgtagtt aatcaccgag
    24361 ttccttctaa tttttttatg ttcttttgtt tcgttttctt ccgagttcgc aaaatctgaa
    24421 ccgtatagca gaagtaacaa cgatgataaa aaagctgtgc gttgggttgt ttccccagga
    24481 aaaagaacag aggcgctggt attgactcta tcttgtggaa taagtgtgct aggcgctaat
    24541 gtccgccaag ttaatttttg acgtttgaaa tcctcattct tgataatctg ataatcatca
    24601 tcgatgccta aaagttgtaa ccaaaaatgg ctaattggat ttttcccaga tcgtgcatta
    24661 tatttttcat aatctacata ttcacctgaa ctcgttattt tggataccaa aatggagttg
    24721 ctatcaaatt ctctagttaa tgaatatttg ccattagtcg attgaataat cagttcaact
    24781 ttactgtatc cggttgcatc ggttgaaaag ggagcagcgt cttttttcgc gccatagaga
    24841 aaatcgattg ccgtaacaat gctagttttt ccggtatttg aagggccaga aattatgttt
    24901 agtccattgg tgaacttgac agttgcatct tcacgatctt tgccttttat ggtaacacgt
    24961 tcaatataat aattttgcat tatcattgct ctcctttaaa ttcctttgcg cgttggttta
    25021 tttgtcgcag aagctcaacg tcacttttat cttgaatgta tttaacagat tcgtcagcta
    25081 tttgtaagta tttttctgca tataccgata cgaattgtga tatcaacttt aggccatctt
    25141 ctgataaagc atacttaaaa ccttctttgc tttgcgatgg ttggataaca tgttgcagta
    25201 caagattttt gactgcatct ttaactaatt cacgtcgcgc aataaattca ctgaatctat
    25261 agctgttatc accatttaag ttgtaatcgg aaatttcaaa gtcgcgacca tacgtggtga
    25321 tgaagtctgt gattaaaatt ctatcagcgc tgacaggctc tttaaattca gagagcagta
    25381 acattactcg aaccgaaatt tcaaatggag tattaaacaa actattcatc ttcagtcacc
    25441 catttgatct ttccgtcatt aaccagcatg tggcaaatgc cttttttagc agaattttgc
    25501 agcaatcccg gtataaacga taagattgat ccgtttaatg ttataacaga cgactgctgt
    25561 agaacggctt tgagtctctc aacgccatca tcgtagtctt cttcatagac ttcctcgatg
    25621 ccgtcgtaca tatcttcttt taaagcttca aagtgctcag ttccttcctc ttgtgtaaag
    25681 ttatcacgga ccccacgacg tatgctttca gctttatagt aatttcttcg ctgtctcttg
    25741 aaatccttta gccgcgaatt tggcaggtcg tttgtatgct cgtatacttc gtatccatcg
    25801 gcatctgcat atgctgaaaa aagagctatg acatacgtca tctcctcagc tctaatatca
    25861 aggggaactg gcaaatcgtc aaagaatttt ttcacttcat cattataaga gaaatcacca
    25921 ttagtgttga ttcttaaact tgcgagctct aattcgctaa gcttcggctt ggcctttttt
    25981 gtttttactt ttgtttgaga agcaattgag gtgatttcat caagtaaaag atgggccact
    26041 atttcagaaa cgtctgtatc tttggggatt accacacctt tatcaatcaa ttgttgctta
    26101 aggacggttg gagcatcatc tataagacgt tcataaataa aagtcgtgca tttgtcgatg
    26161 ttgaattttc cagcaacccg aactgcatac tcttgtgtta atggtcgagt accattgtaa
    26221 aatcttcgta ggttatcatc agaaaaaccg aaaatggtgg cctcgtcatc atttgaaaca
    26281 ctatccagta aagcaattaa aaaatcctgt ggcttcacgt actcgcttcc cataaacgga
    26341 taaagcgttt gagtaatttc acttatttcc aaaatacaac ctcctttgtt acgtccgccg
    26401 ttgaccgccg tttgccgccg atgactgacg aaaacaaggg aggccttttt ctataattaa
    26461 aacatagtag tttaactatt taattttatc atttatggtt cataaacact acattatgtt
    26521 tcaagaaagt gaacgaattt atcttaaaag cgaatagtcc tactaataaa atataacaat
    26581 gcctgagttg caatagggca aggttacaaa tactgaaaag aactggttat ccagaaaatt
    26641 tttcagatat tgtatgcctt actttattgc gctctttttt cgtgatgagg gacggtgtac
    26701 gtattggcac ggtccctgtt tgttttcctt gctcaaaagc tctttcaggc aagaaagagg
    26761 gatctaatga gtagatttat gaaaacacac caaaacgacc gcagcactta caagtacgtg
    26821 tttgagcgta ctgatgccaa tggacgtatc caaaaagaag tggttgagat tcgcccaggc
    26881 gaagacggcg tcactgagct taacattcag gagctgcatc gcgctgacga ccgtgcgatt
    26941 tacaactttt ataaaaacgc acgtcctgaa cgcacagaag aagaaaaaga aaagatcaag
    27001 gcttggaaag agaagttccg tgaagacttt aataatgagc acggctatta tccaatggac
    27061 gaatacgtgg atggtgaagc caacgattac ttcaaacgta actacaattt gtcgctcaat
    27121 gaatttgatg atgaatgcga tgagggggac aaccccatcc aatcattggt ttacgatgct
    27181 taccaagata atgaagaaga tcctcgtatc gatcgtttgc gtgaagtcgt gacgcaatta
    27241 ttaccgtacc accaagaaat ctatcacatg ctcgtcactg aaaacatgtc tcagactgaa
    27301 gtggcggaca agcttgacaa gaaaaaacag tcaatcaatc gcacctttgg aatcatcaaa
    27361 gacaagatca aagaattatt ttagaaaaaa gttacggggg agtttacttt ccccgttttc
    27421 cttcgcctgt attgtgtaag ggagtagatc ctaaacaaat aaagatgaag gaggaccatc
    27481 atgaacctaa aacacaaaat ccgagtaaac gtgactgatg acacgggtaa caaagaaact
    27541 gtcctacaag gtggtgtgcg tcatttgcct cgctggtttg cacggtggct gttcggtaag
    27601 ggcaacatgg acaaaattct cgtgctcaca ccaggggaca caatcgaatc agtcgaaatc
    27661 cgtgaagtta acaagaaggg agcgtaatga cgtgagtaaa acaaaactat ttcttcaggt
    27721 cgttgaggac gtccaagcgt tagctgatag cttacaagag ttggctgtgg cgatgaaaac
    27781 caatgaacct gaaccacaag ttgaaacacc agaaccgaca atggaaacaa aatcagaagt
    27841 caaacaaccg acgcttgaag aagtccgtgg tttgttggca cgtaaatctc aagaaggtaa
    27901 gtcaactgaa gtgaaagcac tgattgagaa atatggtgcc agtcgcttga gtgatgtgcc
    27961 gacagaaaac tacgcggctt tggttgcaga tgcggaggga ttgtagatgc caacacaaca
    28021 cgcagtccta tccgcatctt caagtaatcg gtggattcac tgtccgccaa gtgttcgctt
    28081 gtcagagcac tttgaaaaca agtcgagccc gtatgcccaa caaggcactg atgctcatac
    28141 cttatgtgag tacaaactga ataagttcat tggtaatgac gtcacagacc caacatctac
    28201 gcttgaattc tacgacgagg agatggagca gtgtgctgaa agttatgcga ccttcgtcat
    28261 ggaagaagtc gctaaggcga aacagacaac agttgactct attgtaatag tagaacagcg
    28321 gctcgacttc tcaaggtacg tcccagacgg ctttgggacc ggcgactgtc tcgtcattgc
    28381 ggatggaacc ctatctgtta ttgacatgaa atacggtcta ggaattttgg tagatgcgta
    28441 tcagaaccca cagatgatgt gttatgcctt gggagcgttg gagcttttcg atgttattta
    28501 tgacattcaa gaagtcaaga tgaccatttt ccagccaagg cgtgagaatg tgagtacgta
    28561 cacactgctg aaagaagaat tacttcagtg ggcaaaagat gtgttgtcgc ctgccgcaga
    28621 tttagctttc aaaggtgagg gtgagtacca atgcgggaaa tggtgtggat tctgtccggc
    28681 gaagaatagc tgtcgtgcac gagcagacca taacttgaaa ctggcgcaat atgagttcaa
    28741 accaccagag cttcttagtg atgatgaaat cgaagaaatc atcggtaaag tggatgacct
    28801 ggtttcttgg agtaacgata tcaaggactg tgctttgaaa cttgcactcg gtggcaaaca
    28861 atggactcat cacaaattag tcgagggtcg ctctactcgt aagtattcta atgacaatga
    28921 tgtcgctgcg gcggtcatca aggctggata cgacccatat gataaaaagt tacttggtgt
    28981 cacagcaatg acgaaggcac tcggtaaaga gaaattcaat gaaatcttgg gcgaatacat
    29041 catcaaacca aaaggcaagc tgacgctagt cgatggttca gataaacgac aagcggtcac
    29101 agtaaacaac ttaaacgaag aatttaaacc attaacggag gaacaataat atgtctaaaa
    29161 caagcaaagt aatcactggt acaaacacac gtctctcata cttccacggt tgggaaccag
    29221 catccatcaa cgggggcgca gagaaatatt caacagcagt cttgattcca aaatcagata
    29281 agagaacgat tgataccatt gaaaaagcaa tcgatgcagc aatcgaagag ggacttgcga
    29341 aattcggtgg gaagaaacca aacaaagcag aaatcaagct gccattacgt gatggggata
    29401 tcgaacgcga cgatgaagca taccaaggcc attacttcat taatgcgaac tcaaccactg
    29461 caccacaaat cgtggatcag cacgttcaac caatcttgga tcgttcacaa gtgtatagtg
    29521 gttgctacgc tcgtgtgtcg attaatttct atgcctttaa cgcaaatgga aatcgcgggg
    29581 tagcggcagg gcttgggaat atccagtttg tacgtgacgg cgaaccactc ggctcacaag
    29641 tatctgcaag cgatgagttt gaaaccttgg cggatgatga tttcttggct taatcattaa
    29701 aacaaaaacg gccatggcag aagtgctgtc gcctgtttga ttggaggaag aaaatgaaac
    29761 atttgtcatt ggatttggaa acgtatagta gtgtcgattt atcgaaatgt ggtgtctaca
    29821 aatattcaga gtcccccgat tttgaaatac tgctatttgc atacagtgta gatggtggtc
    29881 ccgtcgaagt ggttgatctg aagatggacg aaactatccc ggctgaaata ttaacttcgc
    29941 tatcagatga aacagtgatt aaacacgcct tcaatgcgag ttttgaacgt gtgtgtttgt
    30001 cacgctttct ggggttggat ggctttttga atccacgagg gtggcaatgc acaatggtgt
    30061 ggggtgcgac attaggtttg ccgttgtcac ttgtaggagt tggtgctgtt cttggtttgg
    30121 ataaacaaaa gctcacagaa ggaaaagacc tcatccgata cttttgcgtg ccatgcaatc
    30181 cgaccaaagt taatggtgga cgcacgagga acttaccaca ccatgcgcca actaaatggt
    30241 cagcatttaa gacatacaat agtcgtgatg tggaagttga aatagctatc caaggaaaac
    30301 tttccaattt tccagtgctt gattctgttt ggaaagaata ctggctggat caggaaatca
    30361 acgaccgagg aattgagatt gataaggact ttgtggaagc tgccgttcag ttggatgaat
    30421 tatcaaaagc tggattgaat caacaattgc aagaattaac tggactagaa aatccaaaca
    30481 gcgtgcttca aatgcgggga tggttactag accacggact tgaaaccgag tcactcggta
    30541 aaaaggcagt ggccgaacta ctcgaacaac ctattgatga aaatttaaag caagtcctgg
    30601 tcttacgtca gcaactcgcc aagtcatccg tgaaaaagta tcaagccatg caaaactttg
    30661 cgtgttctga taatcgttct cgtggtttat tccaattcta tggagccaac cgtaccggac
    30721 gctttgcagg acgtggcatc cagcttcaaa acttgcctca gaaccatatg tcagatttat
    30781 ctgaagcgcg tagtttagta aaacaaagca attttgaaat gcttaacatt ctctacggca
    30841 atgtcccgaa cgtactctca gaactcctcc ggacagcgtt tgtaccacgt gaagggatga
    30901 aatttatcgt atctgacttc tcagcgattg aagcacgtgt gattgcttgg ctgtcgggtg
    30961 agaagtggcg tcaagaagta ttcaaaaatg gtggcgatat ttactgtgcg tctgccagtc
    31021 agatgttcgg tgtgccagtt gaaaagcatg gcgtgaatgg gcatctacgt caaaaaggga
    31081 aaattgctga gcttgctcta gggtacggag gatcggtggg tgcattgaaa gcaatggatg
    31141 ctcttgaaat gggactaact gaggatgaat tacagccgct tgttaattct tggcgagact
    31201 caaacattaa cataacggag ctttggtggg ctgttgataa ggcagtaaag acttgtgtga
    31261 aagaacgacg gagcacagcg acgcatggca tcaagtttga atacaagtcg ggttttctgt
    31321 tcatccaact cttgtcagga cgtaaattag tttatgtaaa accaaagatt ggtgagaaca
    31381 aattcggcgg tgaagctgtt acctatgaag gtgtcggtgc taccaagaaa tgggaacgtc
    31441 ttgaaagcta tggtcccaag tttgtcgaaa acatcgtgca ggcaatagct agagacattc
    31501 tctgtttcgc aatggagaat cttcgtaagt atcgcattgt cgggcacgtc cacgacgaag
    31561 tgattatcga agtgccaatg gaatcgaagg ttgaagacat ctgtcagttg atgagtcaaa
    31621 cgcctaagtg ggctgaaggc ttgctactga acgctgatgg atacgagtgt gaattctatc
    31681 aaaaggatta gtcaatgtgg agtcagaaat ggctccatat ttttttcaaa tttttttatc
    31741 aaaaataagg ttcgaattgg gttctttctt cgcttataga cgaaggcaaa aaaatcaaaa
    31801 ataaattcga gtcagagttt actttcgacc attttcttcg cctgtaagtt agaaggggca
    31861 aaatgcctct cctgaaaatt ctattacagg aggttcacaa gatgaacgaa ttgaaagttt
    31921 ttgaaaatgc ggagtttggc tctgtacgca cggtgatgat tggcgatgtc cctcactttg
    31981 ttggtaaaga tgtagcatca attcttggct acacaaatcc acaaaaagca ttacgagatc
    32041 atgttgatga ggaagataag acggtgaacg aatcgttcag cgttaatggg acaatggcta
    32101 tccttatcaa cgaaagtgga ttgtactcac tcatcatttc tagcaagatg ccgaacgcga
    32161 agaaattcaa gcgttgggtg acaaatgagg tcttgccgtc tatccgccaa cacggtgtgt
    32221 acgcgacaga agatttcatc acaaagtcaa tcgaagatcc agcatgggcc atttcagtgt
    32281 tgaagcagct acaagagaaa aaagaaatgg tggcgatgca acaacaaatg attttggaga
    32341 tgaaaccaaa agttagctac tacgatttga ttcttcaaaa caattctgtg atgtcgattt
    32401 ctaaaatctc gaaggactac ggtatgtcac cacaggcaat gaacaagttg cttcatgaac
    32461 tgggaattca gtacaagcaa ggcaagatgt ggttgcttta tcagaagtat gccaatcaag
    32521 gctacacaca atcaaaaact catgcgatag atgcagagcg cagcgtgatg catacctact
    32581 ggacgcagaa aggtcgtttg ttcttgtacg acttgctgaa atcgaagaag aacatcttac
    32641 cagtgattga gcgaggtgat aataatgcct agacgtaaag attacagaac atcagtctat
    32701 gtctgctcgc cgttctcagg aaatgttgtc gtcaaccata aaaacactaa gcgttactgc
    32761 cgtattgctc ttgaccgtgg ctacatgcca gttgcggcac atctgatgta tccacaattc
    32821 atggacgacg caacggagcg tgatgtggca atgcacatgt cacttgttct catgggcaaa
    32881 tgtgaggaag tctgggtggt gggtgacacc atctcccaag gcatggcagt tgaaattgaa
    32941 caagcggagt attggaagaa acatatcagg tacttcgatg atgagatgaa ggaggtctca
    33001 aatgcttaag atgacgttat tcacatccaa tcaggtgggt aattccaaga atggattgtt
    33061 cccaaccaag caagtggtga cagataaaga aacacttgtt gccacggtca agctggatca
    33121 tgtgatggct gagtacaaag gcaactatcg caaaggtgac aacttcatcc agtctgatgt
    33181 gattccactt gattgcgaca atgaccactc agacaatcca gatgattggg tagccccttt
    33241 cgaagtggcg atgcagtttc ctgatgtgac gtttgcggtc agctattcac gtaacaacat
    33301 gaaagacaaa ggtggcaaat cagcacgtcc acgttttcac gtatatttcc cgattcctgc
    33361 cacaactgat aaagatgaat atgtggaact caagaagcag attcaaagtg agttcccata
    33421 ctttgacaac aatgcgatgg atgcggcacg tttgcttttc ggaacaccaa actctgaagt
    33481 tgaaatctat gaaggtagta agttgatcac tgattttctc gaagatgatc tgtttgctga
    33541 ttgggataat gcgcagtcag agattagcga aggcagccgt aattcaagca tgagtcacat
    33601 cgcaggaaag ctcatcaaac gctacggtgc aacggaagat acgtatcaca agtatctcga
    33661 acaatcccaa aaatgtaatc cgccactgcc ggaggaggag cttaaagcta tctggaatag
    33721 cgcggtcaac tttgggaaga aagtgaaaca acaagaaggc tacattcctc cagagcagta
    33781 caactctgat ttatcacttg agccaaccga ctactcggac ttaggtcaag caacggtctt
    33841 agctcgcgag tatgaatgta agcttcatta ctcaccgtca actggttacc ttgtctacaa
    33901 cggcagctat tgggaagagt cgaaaccaaa agcgcaaggt gtgatccacg cattgacaga
    33961 acgtcaactg gaagaatctg aaaccgaaat tgagaagcgt accaaggaaa tggttagtaa
    34021 tggcgcgttc gatgtacttg ctagtgttgg tccgaaaaag gcagtcacca tgttcaacac
    34081 agcgcaacgt catagttttg atttgtacca acacgcacaa gagtataaga agtttgctgt
    34141 caaacgccgc gacagcaagt atctctcgtc agcattaacc gaggcaaagc ccatgcttga
    34201 aatcgagcaa cggttactag atgtgaacga gttcttgctg aacacaccaa cagcaacctt
    34261 tgatttgcga acaggcaaat cacaagacca caactcagaa gattacatca cgaagcaaac
    34321 ggaatgtgcc ccgagtgacg ccaatcagca gatttggttg gacgctctga acaccatttt
    34381 cgtaggtgac caagagttga ttgattatgt ccagatgatt gtaggacttg cagctattgg
    34441 taaagtttac gttgaagcgc ttatcatctc gtatggtgaa gggcgtaacg ggaagtccac
    34501 gttctggaat gtcatcagcc gtgtgctcgg taattactct ggcagcatct cagctgatat
    34561 tcttacaagt caaatccgac gcaatgtgaa acctgaactt gcggaagcga aggggaaacg
    34621 cctactcatc gcagccgagc ttgaagaggg gatgcgactc aacacatcta acattaagca
    34681 gctctgctcg acggatgaaa ttgcggctga gaagaaatac aaagacccat tcaagtatgt
    34741 cccaacgcat actttggtgc tctataccaa ccacctccca aaagttggag cgattgataa
    34801 aggaacctgg cgtcgtttaa ttgtgattcc attccttgct acgattgacg gcaataagga
    34861 catcaagaat tatgcggact acctttttga aaatgccggc ggagccattt tgcaatggat
    34921 ccttgacggt gcgaaaaaag tcatcgccgc agactttcat ttaacggttc cacaagttgt
    34981 cgctaatgca atttctgaat ataagacggc gaatgattgg cttggccatt tcttagacga
    35041 gtgctgcgag actgctgttg actttgaaga aaaatctggt gagttgtatg cagagtatcg
    35101 tgcgttttgt acgcgaactg gtgagtacat tcgtagttca gccgattttt acacggctct
    35161 ggaggcgggc ggatttaagc gccggcgaac taataaagga aacaagattg ttggtttgca
    35221 actcaaatcc gagttcttgg aatagttgat tttaaaggaa agtgtaacct gtgtaggtct
    35281 tatatagaac ttttcttaag ggataaaaaa ttaccaccta tataaagtta tgtattgcgt
    35341 attcacgggt tacaccatac ttgcagattg gagaatatct aaatgaaatt taaacagtgg
    35401 ttacaaattg gaatcggtaa acgtgattta cgatttgatg agttttacga gtttgccatg
    35461 tcggatgtca cgtatccttg gaagaaatca ttcgataagc aagtcgcaca tctgatgatt
    35521 cactgcgcag aaaaggaata cttggaaatc ttgcgtgatg cgtatttcga gtatttgacg
    35581 gtgtggctat gagagaaaag caggttgagc aagcactagt gaaagccgtc aaaagggtag
    35641 gtggtatttg cccgaagttt acatcaccgg ggcttgctgg tgtgcctgac cgattggtac
    35701 ttatgccaaa tgggaaactt ggatttgtcg aagtgaaggc gccagggaag aagccacggt
    35761 ctttacagct ttttcgtatg aaacaactga cagatttagg gtttcagtgt ttcgtgcttg
    35821 atgagattga tcagatacct gagttactag aaagaatagg aggtgatgtc tgatgaagtt
    35881 cgtaccacat aactaccaga aatatgcgat tgattttatc aaagatcata aaactgccgc
    35941 ccttctattg gacatgggct tgggtaaaac ggtgacaacg ctgacagcta ttaaagactt
    36001 gatgcatgat gactttacaa tacaacgtgt cttaattatt gcaccgttgc gcgtgacgca
    36061 atcaacatgg ccaactgaaa ttcaaaagtg ggatcatttg aaagatttat cgtattccgt
    36121 ggttcttgga acaccaagtc agcgaatggc agcattacaa aataatgcca acatctatct
    36181 gatcaatcgt gagaacttgg actggctgat taacaaatcg ggttttacgt ttgattttga
    36241 tatggtggtg attgacgaac tgtcgtcctt caaaaactta aaagctaaac ggttcactag
    36301 ctttatgaaa gtcagacaca aggttgaccg aattgttgga ttgaccggaa caccttcaag
    36361 taatggtttg atggatttgt ttgcggagtt caaggttctg gatatgggcg agagattggg
    36421 ctattatatt tcacgttatc gagataaata ctttctacct gacaagcaaa atggcatgca
    36481 gatttattct tggaaaccaa gggaagatgc agaacaagaa atctacgaca agatttcaga
    36541 tatcacgatt tcaatgaagt cagtcgattt cttggatatg ccagagcttg tgatgaatga
    36601 agtgccagtt gatatgggac tcaaagaaaa gcaaaagtac gatgaaatta aggcagactt
    36661 ggtgctacaa ttgaaagaag ccaccattga tgcagccaat acagcggtgc tttcgaataa
    36721 actgctacaa atggcgaatg gagcaatcta tgatgagttt aatgtgagtc atcacatcca
    36781 tgaccagaag ttagatgctt tggaagattt gattgaaggt acaaatggca aaccgattct
    36841 gattgcctat tggttccagc atgatctgga gcgtatcaag gaacgattca acgttcgaca
    36901 gatcaaaacg tctcaggaca ttattgattg gaacgaaggt agcatcccca tcgctgtcat
    36961 tcatccggct agtgcaggac atggattgaa tttacaagct ggtggttcaa cacttgtatg
    37021 gtttggtctt acgtggagtt tggaacttta ccaacaaacc aatgcaaggc tgtggcgaca
    37081 agggcaaagt gatactgtgg tcattcatca cattattgcg aaagatacga ttgatgaaga
    37141 tgtgatgttg gctttgaaac gaaaagataa aacacaatca tgtttgattg atgcagtgaa
    37201 agctagattg gagggaagca agtgacacca aaagaatact tgaaacaagc gtaccgtttg
    37261 aataaacgaa ttgacgaccg gattaaagag aaggcggagc tctatcaact ggcggtgaca
    37321 gtttcttcca caacattcga agaaaagatt tcaagtggca cacggaatac tgaggcacca
    37381 tttgttcgta accttgagaa gtgttaccaa ttggaagctg aaattgatgc agagattgat
    37441 aagtttgtag acctcaaacg acaaatgcgt ggcgtgattg atgaactcac aaacgtggac
    37501 gaaaagattg tgttaatcaa tcgatacctg ttggaagaga gctgggacaa gattgctgaa
    37561 gatcttcacg ctgacgtgcg gacagtcttt cgttggcata gcaaagctct tgagaacttg
    37621 aagctaccag atgaacccat agtcatttga aatgtgtcag tgaatgtcag tagcctttag
    37681 tgatatcatt aagatgtcaa aaaagacaaa cgatactcaa gcaataccag caatagaagt
    37741 atcgaacaaa tactcgaagc ctttgtggga aaaatccttc aagggctttt cttttgcccg
    37801 aaaggatgtg accccacttg ccaatgaaac cagctaaccc ctgtaagttc ccaggttgtc
    37861 cgaaccgtac gcatgatact tattgtgatg aacataaaac acttgcgagg aaacgttacg
    37921 agaaatacga gcgtgatccg gcaatcaaca aacgttacgg cagagcgtgg aagaaaatac
    37981 gggcaaggta cgtggcagca catcccctat gtgagatgtg tctgaaggaa ggacgcttta
    38041 cttcggttga gattgtgcat cacatcaagg aattaagtga tggtggtacg catgatgaaa
    38101 ataatttgat gagtgtctgc aaatcatgtc actcgaaaat tcacatgact cgacgaaaca
    38161 caaaagataa aacaatcatg tgaaaaataa caaggggggg aggggcggta attatcccta
    38221 aacccttcta aactgtacag cgcgctaggc ccttacgcga aaaaagttca gttcaaacgg
    38281 gggatagccc caaacaagaa aaagaggtga taaattttgg caaaagatgg aacacaacgc
    38341 ggtggcagac gacctcgttc aggggaacgc tcacagccac tcgttgataa agttgctgct
    38401 ggaaagaaag catcgcggat ggaatttgac ttaccggatg ttgaattatt aattggtgaa
    38461 gatattggtg agggtgtaga gcttgagggc aatgattatc ctgaaccaag tgcttacctt
    38521 tctgcaaaac agaaggatgg cactgtgcta ggcgctgatg aaatttataa ctcaacgttc
    38581 caatggttga aagaccgcgg atgcgaaacg ttgatagccc cacgaatggt tgagtcctac
    38641 gctcagaact ttgcgcgttt tgttcaatgt gaaaatgctc tgagtcaata tggtttgctc
    38701 ggaaaacacc cgactaccaa ggcagtcatg gcctccccat ttgtggcgat gtcgcagaac
    38761 ttccaaaagc aagcaaatgt gacgtggtat gagattcaac agattgtaaa agagaatgca
    38821 tcagtggaat acacaggaga tccgaatacg gatgcgatgg aacgattgtt acgttctaag
    38881 aatggttgaa aggaacagaa tttatgatag agaaggttaa tccgcaacat ccagataaag
    38941 ttgcagatcg tattgccgga gccattgtgg acttggcata cgcagaagaa aataatccaa
    39001 agatagcagt tgaggtttta attggtcatg gcatctgtca tgtgattatt gaaacttcaa
    39061 ctgctttaaa tttgaatgaa atcagccaag cgattaatcg tatcgcaggc aaaattaaaa
    39121 aagatgtggt gattgtgccc caagataaag agttggctga caatcaagcg actgaaattc
    39181 gttgtggcga taatggaatc tttcgtggga tgccattgac tgatgaacag ttacagctct
    39241 caaaaattgc gcgtgactta tatcaggacc atccatttga tggaaagtac attcttgatg
    39301 gtgaacgctt gataatttgt cagagccacg caaagacaaa tgagttaaaa caaaaatatc
    39361 caaacgctac agtcaaccct ctaggtgatt ggactggcgg cccggatgtg gatacaggtg
    39421 cgactaatcg caagcttggt tccgatatgg cagacggagt aacaggtggc ggtatccatg
    39481 ggaaagactt atcaaaagct gatgtgtcag tgaacatcta cgcattcttg aaagctcaag
    39541 aaactggtat gcccgttgag ttaagctgtg cgattggtga cactgagatt gatggcgtgc
    39601 cttattctga aatcgtggaa gttgcgcgtg attatgtgaa tgagcttggc gggtttgaga
    39661 aactagcaga atgggggttg ttctaatgaa cacaacagaa cgttttgaga aagttgatat
    39721 tgataagctg gtcccttatg ccagaaatgc ccgtacccac agtaaggatc aagtgaaaca
    39781 gattcaagct tctatgcgtg agtttgggtt cataaatccg gtgattgttg ataaggatta
    39841 taacttgatt gcaggtcacg gacgtattct tgcggctaaa gaagaaggta tcaagcaggt
    39901 accgtgcgtg tttgtggaac acctaaccga ggcacaaaag cgtgcgtaca tacttgctga
    39961 taaccgacta gcaatgaacg ccggttggga tgatgaaatg ttggcggttg agttatcaga
    40021 cctgcaaggt gaagagtttg atttggattt gcttggtttt gaaactgatg aacttgataa
    40081 attactcaac ggtaatgatg atgcccaaga tgatgacttt gatgtagatg aggaacttga
    40141 aaagccagcc ttcagtaaaa caggggactt gtggttgctt ggtcgtcacc gtttagtttg
    40201 tggtgactca acaaaacctg aaacttatga gaaactcatg gacggcaaac tagcaaatct
    40261 tgtggtgaca gatccacctt atggggtctc atatcaaggt aaagcgggaa cgattaaaaa
    40321 tgataatctg aaggacgagg cattttacaa cttcttgtat gatgcctttt cttgtatgga
    40381 aagtgtgatg gccaacgacg ccagtattta tgttttccat gcggatacgg aaggcttgaa
    40441 cttcagaaaa gcattcgtgg atgcagggtt ctatttatca ggtacgtgca tctggaagaa
    40501 acagagtcta gtgcttggtc gttcgcctta ccaatggcag catgagccgg tgctctatgg
    40561 ctggaagaag aaaggcaaac atcaatggta caccggacgt aaggaatcga ccatctggga
    40621 atttgataaa ccaaagaaga atggtgacca cccaacgatg aaaccaattc cgctgttggc
    40681 atatccgatt accaattcgt cactcagcaa ctgcatcgtg ttggatagtt ttggtggcag
    40741 cggatcaact ttgattgctt gcgaacagac cgaccggatt tgccatacga tcgaactgga
    40801 tgaaaagttc tgtgatgtga ttgttaagcg ttatatcgag caggctggaa gcgatgcaga
    40861 cgtttcagtg attcgagatg gaaagactat cgcatttaat gacctagaaa atcctgaata
    40921 aataacgtat cacttgctat aaaatccctt tagagtgata tatagactac caaaagaata
    40981 cggaagacaa agttatgttt ccaactcatg aacaaatcga gggtatgaaa aaacgttacc
    41041 ctgaaggtag tcgtgtagaa ctcatcaaga tgaacgaccc acatgcccca gcgaagggca
    41101 cacaaggcac agtaaccggt gttgacgaca ttggttcaat ccttatccaa tgggacaacg
    41161 gttcaacact gaacgcggta tacggcgaag atgtggtgca catcatcaaa ccagacttcg
    41221 aattggtctt tcaaaacggt agaagagaag gatttgaaac cttcaatgat gcttggcaat
    41281 acgtgacgga catgatattg aaccacgatt tggtctgggt tgaccttgag tcaaaaacag
    41341 aagataccat ctacatcaga aaggggcttt aagatgaaag tgagtaaacg taagatttac
    41401 aacatcgcaa agaattacat cgttcagttg cctgaacgtg gcgacctgaa ggcccacaac
    41461 agcgaccgtg aagacttctt ggacattgcg gtttggtgtt tggaagatgc cttaattgcg
    41521 gcctatgagg caggcagaaa ggacggtcaa aatgaacgca aagattaagc aacaaatcat
    41581 tgagattgca gaaagtggtc tgacaaatat gtttgatgcg attgcagtgc agcgaattgc
    41641 acatgaccgg cacttttacg agttggtgat tttcattgaa gaacacaaag atgagtacag
    41701 ccgctttatc ttggagggtg ataaatgatg gattatcaaa tttacatgcc agataatgaa
    41761 gatggcattg aagaaggata ctacgattgg caagaactgg tgcaattgct gcgggataac
    41821 aagaacaacc ctgaagccat tcagttcatt gccgatatga tggaataaag agattaaggc
    41881 agctgacaac agctgctttt tgcgtagaag ggagtgagtt ccaatcgata attacaaacc
    41941 aactgaattt atggacaaag actcccatta tgataagtca cgtgcagatt atgccgtggc
    42001 ctttattgag ttgttgaaac acacgaaagg tcagtggttc gggaaaccgt tcaaactgat
    42061 gccttggcaa gaaaagatta tccgtgactt attcggaacg attaaggcaa atggctacag
    42121 acaattcaat acggcttatg ttgaaatacc taagaagaat ggtaagagtg agttagcggc
    42181 tgccgtggct ttgcttttga cttgcggtga tggggaagaa cgcgctgagg tatatggctg
    42241 tgctgctgat cgagcgcagg cttcaatcgt atttgacgta gctgttgata tgattcgcca
    42301 gaacccagcg ttaagcaaac gagtgaaaat cctgacttca caaaaacgaa ttatttatca
    42361 gccaacgaat agtttctatc aagtattatc agctgaagca tactctaaac acggattaaa
    42421 cagttctgcg gttgtatttg atgagcttca cgcacaacct aacagaaagc tgtttgatgt
    42481 catgacgaaa ggggcagggg atgctagaac gcaaccgcta ttctttttga ttacaacggc
    42541 agggaatgat accaattcaa tttgttatga gcagcatcaa aaagctctcg acattattga
    42601 aggtcgaaag catgataaaa cattctatcc gttaatttac ggtatcaagc aagatgatga
    42661 ttggactgat ccagaagttt ggaagaaagc caatccgtca cttgggataa cgattgcaga
    42721 agaaaaggtg gtggctgctt gtgattcggc taaacaaaat ccagctgaag aaaactcatt
    42781 cagacagctt cgcctgaacc aatgggtgaa acaagcagtg cgttggatgc caatggataa
    42841 atgggacgct tgttcattca atgcagatga agaaagtttg agaggtcgag tttgttacgg
    42901 aggtctcgac ctttccagca cgaccgacct tacatccttc gttttggtgt ttccacctga
    42961 agatgaggat gacaagtttg tgatactgcc atattactgg gtgccagaag ataccttgga
    43021 cttacgagtt aaacgcgacc atgtgaatta tgatttgtgg gccaagcagg ggtacataca
    43081 aaccactgag ggaaacgtag tccactatgg tttcattgaa aaatttattg aggaactggg
    43141 tgagaaatac aatatccggg agattgcctt tgaccgttgg ggagcagttc aaatggttca
    43201 aaacttagaa ggtatgggat ttactgtagt tcctttcggt caaggtttca aggatatgag
    43261 cccggctact aaagagttga tgaagttgac tcttgaacag aaattagccc atggcggtca
    43321 tccggttctt cgttggaata tggacaacat tttcgtccga actgacccgg caggtaatat
    43381 caaagctgat aaagaaaaat ccactgagaa gattgatggt gccatcgcga caattatggc
    43441 tctggatcgt gcaattcgtt gtggaaatga taatggtgcc agtgtttatg atgatagggg
    43501 attgttgttt ctgtgaaata tgagctttta ttacaatcca ttgatataat tgtctttgga
    43561 ggtgtatgtg tatgaatgaa tatttgattc aagtagcacc agataaaata tcttcagacc
    43621 cggacaacac attaatttct agactgtttt atagaaaatg gtggggaaaa attctaactg
    43681 aagatttgac aaagtatgct gaaggaattg ggaattatag gggagaaacg attatttctt
    43741 tcaacacaat tgctggtgca acgatacggt tattgaaatg ctataatcaa aagaactttt
    43801 caatgccaga tagtcattat gggcgtttga aagtcatttt atattccgat gatatcaccg
    43861 aagagatgaa agtgaaattt gtaaaattct atgatttaag tcatactctt gcaaatttta
    43921 tgccactata tgtatcaaaa ccttctctca atggtgttaa aggtaaaggg ccgtatcatg
    43981 actttcctga tttattttat tctgatgtaa gagataaacg atatggttca tcaactaacg
    44041 ctgataaatt tatcgttaat aataaagact atttctctaa attcaaagat tggaaaagtt
    44101 ttatagaact aaattattta caagatttct tcgatgatga agattatatg acgtttaaaa
    44161 aacttgcacc aagtagttca ttctttcctt atcaaagaaa atccttaaca cctgacgaaa
    44221 aatcgaaatg tttagaggaa gtaaatacct ttttaaataa tgcaattgag ataattttgt
    44281 caagaggaag tcggttttat atctaatgtg aacacagcat ctacaaatcg taggtgcttt
    44341 tttgatactc aaattttgaa aggagcaatc aatgggcata ataaaatcac tattcaaatc
    44401 acgcgataaa ccaacaaatc aaaccatcat gaatccatac cgatttatgt ttggcggctc
    44461 atcagctggt aaaagcgtga ccgaacaaac agctatgcag atgacggctg tgtactcatg
    44521 tgtgcgcgtg ctatcagaag ccgtggcagg attgccatta cacttttacc aataccgaga
    44581 tgctggcggc aaagataaag caatgaaaca ttcgttgtat ttcttgctgc atgatgaacc
    44641 aaattcggaa atgacatcat ttgtatttcg tgaaacctta atgactcacc tgctactttg
    44701 gggtaacgcc tatgcgcaaa ttatccgaaa tggcaaaggt gaagttgtag ctttgtatcc
    44761 tttaatgcca gataagatga cggttaatcg cgatgacaac aaggaaatct attatttata
    44821 tcaagctgat gtcgggcaag tcagactttc aaaagctgac gtgcttcaca ttcctggcct
    44881 tggctttgat ggcttggttg gttattcacc aattgccatg gctaagaatg ctatcgggct
    44941 tgcgattgca acagaggaat atggttctaa attttttgcc aatggtgcca atcctggtgg
    45001 tgtcctagaa catcctggaa ctttgaaaga cccaggaaag cttcgtgaaa gttggaatgc
    45061 gacatttggt ggcagttcaa atgcggccaa ggttgcagta cttgaagaag gcatgaagta
    45121 tacgcctatt tccatgtcac cagaacaagc ccagttcctt gaaacacgta agtttcaact
    45181 ggaagaaatc gcacggatat tccgagtgcc ccttcacatg gttggcgact tagaccacgc
    45241 gaccttctca aacgttgaac atttgagttt ggattttgtg aagttctcat tatcaccatg
    45301 gctcaatcgt tgggaacaag cacttaataa atcactgtta agtcgcgatg aaaaagaaaa
    45361 gtatttcata aagtttaatg tggatggctt attgcgtggt gactatgcca cacgtcaaca
    45421 aggctatgca accggtattc aaaatgggtt cctatctcct gatgatgtgc gtgaacttga
    45481 ggacttgaac ccaatccctg gtggtgctgg tgcgaaatat tacttcaatg gcaatatgct
    45541 gccaattgaa caagccgggg cagcttatgg cgtaaacaaa gaagaaagcg aggaacaaga
    45601 tgaagaagtt ttggaagtgg aagaatcaaa cggttctgaa tcaagacaac caagaggaaa
    45661 tcctagagag aaccctctac ctcaacggac caatcgctga ggagagctgg tttgatgatg
    45721 aagtcacacc gcagattttc aaagatgagt taaattcagg tggaggtaac gttacagtct
    45781 ggattaattc gggtggtggc gattgtgtcg ctgccgcaag tatttacaac atgcttgctg
    45841 actacaaagg taaagtcaca gtcaagattg acggcatcgc cgcttcggcc gcaagtgtta
    45901 ttgcaatggc aggtgacaag gtactcatga gtcctgtggc gatggtgatg attcacaatc
    45961 cgtcagcggt tgcgtttggc gaaaaagttg atatggagaa agccattgcc atgcttgatg
    46021 aagtgaaagc aagtattatc aacgcctatg aacgtaaaac gaatttatcg cgtgtgcaac
    46081 tggcacatat gatggatgcg gagacgtgga tggacgcaaa taaagctgtt cacctaggat
    46141 ttgccgatga aatcatcggt gagattgacc cggatattcc ggttaataat tcgatgtact
    46201 cggaagtcaa agtaactaat tcactgactt cagcgattgc taagaagtgt cgtattgaac
    46261 cacctgaacc aaaggaacga gaagaaatta ataacactat cccagccgat tcgttaatga
    46321 gtcggcttaa tttaatgaaa aattggagga actaacccat gaacaaaatt caagaattga
    46381 tggaaaaacg tgcacaagct tggaacggag caaaggcttt tgtagaagca aagcaagata
    46441 aagatggctt gatgtctgaa gaagatgcca agacttatgc ggaaatggaa acaaaagtga
    46501 agaactttac gatggaaatt gaacgtatgc agtcaatgga agcgatggat cgtgaacttg
    46561 aaaagccaat gagccaacca ttaacttcgc aacctatgac tactgaaaat aaaatggaac
    46621 ctaagaaaac aggacgtgct tcagctgaat ataaagaagg tgtacttgaa gccttgcgct
    46681 caaacttccg acatgtttca aatgttcttc aagaaggtgt ggatacggca ggcggttatt
    46741 tggtgccaga agaatatgac tcacgtttga ttgacggcat gacttctgaa aacatcatgc
    46801 gtacacttgc gacaacaatt acgacaagtg gtgaacacaa gattaatatt gcaggttcta
    46861 aaccagccgc ttcatggatt gaagaaggtg gcgagttgag ctttggggat gcaacgttcg
    46921 atcaaatctt gcttgatgct cacaaacttc atgtggcaat caaggtgact gatgaattgc
    46981 tttatgacaa cgcctttaac cttgaaaact atatcatcga ccaattcacc aaagccttag
    47041 caaacgcgga agaagatgcg ttcttgaatg gtgacggtgt tggtaaacca ctcggtattt
    47101 ttgcagcaac aggtggcgga caagttgcca ttacaactaa tactcaaagt gcagttacgg
    47161 cagatgatgt ggtgaacctt gtgtatgctt tgaaacgtcc atatcgtaaa aacgcgaagt
    47221 ttatcatgaa cgaccaaacc atcagcttgc ttcgtaagtt gaaagacaac aatggtgcct
    47281 acatgtggca gccagctttg gtagcaggtg aacctgaccg tctgctcggt tatccagttt
    47341 atacttcgga gtttgttcca accgttaagg caggtgcgcc agtcattgct tttggtgatt
    47401 tcagctacta taacattggc gaccgtggcg tgcgttcatt cgaccagttg cgtgaactct
    47461 ttgcaggtaa tggcatggtt ggtttcttag ctaaagaacg tgtggacggt aaacttgtgt
    47521 tacctgaagc agtgcaaatc ttgaagatga aagcatctgc ctcatcttaa ggagtagcct
    47581 atgagtgact tactaaataa ggtcaaggca aacctcatac ttgagcataa cgaagatgat
    47641 gagctgttgg aacgattgat tctgaccgct ctttcttatg ctgagagcta tcaacatatt
    47701 gagtctggct actattcaga acatgaaatg ccgccaacca ctgaacaggc ggttattgtg
    47761 ttagccagta acttttacga aagccgtgat ggttcaactg gtggcttctt tgcggataac
    47821 gtcaatgcca gtaagcaagt ctgggaaacg gtcaatctgt tactcaggct cgataggcgg
    47881 tggcaagtat gagttttgga aagatgaatc aacgaataca aatcgttaaa gtgacgaacc
    47941 aaaaggatag cgctggattt tcagtgaaac aagatgaagt tcttgcagct gtccatgcgt
    48001 acaaagagga aaagaatgcg actgagaaat gggtaaacat ggcgactttt agttcagcga
    48061 cctgcctgtt tcggattcgt tatattccta atctagaggt tacgacagag atggttgtca
    48121 tttcagatca gaaaaggtat cagattgtgt cggtggagaa tgtccgaaat cgcaatatgt
    48181 accttgaaat gctgactact gaaatggagg tgagtgcgga tggctaaagc attgatgaag
    48241 atgccagaag atttcttgtt aaaggtttca aaactagaaa acaagacaga tgaaatcatc
    48301 cccaaggttt tagaaagtgg tgctgaagtg gtggaaggca aagtccgaag taacttaact
    48361 gccgttgttg gctcaaatac aaaagtacca agtcgttcaa ctggtgaact tgaatcagcc
    48421 cttggtatca cacaagctcg tcaagataaa agcggcaact ggaacatcaa ggttggtttt
    48481 gatgagcctc gaatcgatgg cagttcaaat gctaagattg cgaacattct cgaatatgga
    48541 aaacacggtc agccagcaaa gccattcttg aaacctgcaa aatcacaaag tcgcaagtca
    48601 tgcattgaaa caatgaaatc aaaaatggaa agtgaggtaa acggcatttg aacattttag
    48661 aggaattgaa cagtcttttt gccacacttc atattcctgt tgaaacgggc gagtttagtg
    48721 ataaagcgcc agacaagtat tgtgtgctga caccactcgc tgacaggttc gatgtttacg
    48781 gggataactt tccaaccgtt gatgtacagg aagttcgcat ttccttgttt gtgaaaggca
    48841 actatctgaa cacgcaaaaa caactggttc agaacttgtt gaaacacgag tttacgatta
    48901 ctgaacgatt atttgtagga tttgaaaaag atacaaatta ctatcatgtg gcgattgatg
    48961 tcgccaagca ttatgaaatg gaggtctgaa aatggcaacg attggattgg ataaattata
    49021 ttatgcaccg attacagagt cggcaactac tggcgaagaa acatacggca cacccgctat
    49081 gcttgcgaaa gcaatctcag ctgagctttc ggtggaactt gctgaagcaa ctttatacgc
    49141 cgatgatggt gcatcagaag tagtgaaaga atttaaaaat ggaaagttaa cgctcggtgt
    49201 tgatgatatt ggacgtgctg tggcggcagt gcttgttggt gcaaccgtgg atcaaaacgg
    49261 tgtcttaatt tcgacaaccg aagatggtgg cacaccagtt gcgattggtt tccgtgctcg
    49321 taaatcgaat ggcaaataca aatatttgtg gttgtaccga gtgaagtttg ccgtgcccag
    49381 cacaagccta gcaacaaaag gtgacgacat tacattctca acaccaacaa ttgagggtac
    49441 agtcatgcgt cgtaacaagt tagatgctag tggtaagcat ccatggaaag cagaagttga
    49501 tgaaaacgat acgggagttg aacaaacaac tatcaatgga tggtacacga gtgtttatga
    49561 accagatttc acagcagact aggaggcaac aatgagtgaa gaacgcagtg ccaagattag
    49621 ccttggcgga gatgaatatg aactgatttt gacaacaaaa gccacgaaag aaatcgccag
    49681 tcactacggt gggcttgata accttggtga gaaattgttg aaatccgaga actttgaatt
    49741 ggcgcttgat gaaatcatct ggttgattac gctgttggcg aatcagtcca tcaagattta
    49801 caacttgaag aacacagaaa agaaagatga actcacgact gaatatgtgg agttgatgac
    49861 gagtccgttg cagttggctg aatataaatc agcaatcact gaagcaatgt tcaagggcac
    49921 agctcgcaat atcgaaagtg aaacagattc aaaaaacaaa gcaggcgagt aagtgatgaa
    49981 gaaacattta ctcgcctgta ttactatggg acagttcaaa tgggtatgac gtcagatgat
    50041 ttctggtact gctcgcttgg actgtttttg gatttatggg agtgtcataa gcaatttact
    50101 gggattagca agccgaaagt tgagttattc attgatgatg tgataccgag tggaatttaa
    50161 gggaaggtgg tgatttattt ggcagataat tttggtttaa agattggcgt tgaaggtgaa
    50221 aaggagttta agaactcgtt gcgtgaaatc aaccaatcat tcaaggtgct aggttcagaa
    50281 atgaaactgg tatcatccga atttgataaa aacgataaaa gcatcagctc actgtctgct
    50341 cgcaaccaag tcttaaacaa atcgattgat gcccagaaag aaaaaatcac cacccttgaa
    50401 aatgctttga agaacgctag tgacagtttt ggtgaaaacg ataagcgtac tcagaattgg
    50461 acagtgcaat taaacaatgc aaaagcccaa ctcaataata tggagcgtga agttgctaac
    50521 aacgagaagg caattgacgc catgggtact gaagaagttg atgctgctaa gaaaactgat
    50581 gagcttggca acgaactcaa ggatacaggt gacgaggctg aaaagtctgg aagtaaattt
    50641 gaaaagttcg gtggcgttct aaaaggtatc ggggtagcaa tgggtacagt ggcacttgct
    50701 gctggcgctg ctgccgtgga gctaggaaaa caagtggtat ctgcttacgc ggactatgaa
    50761 caattagtcg gtggggtgga tactcttttc aaggatgcgt catccaccgt tcaaaagaat
    50821 gccgacaatg ctttcaaaac tgccggaatg tcagccaatg aatacatgga aactgtcaca
    50881 ggtttctcag ctgccttgat ttcatctctc ggtggtgata ccaagaaagc cgcaggagtt
    50941 gcggatcaag ctgttactga tatgagcgat aatgcaaaca agatggggac ggatattgag
    51001 tccatccaga acgcctatca aggtttcgcc aagcaaaatt acgggatgct ggacaacttg
    51061 aaattaggtt ttgggggcac gaagtccgaa atggaacgtt tgctccaaga ggctgagaag
    51121 atttctggtg tcaaatatga tatctcgaac ttcgctgatg tgactgaagc gattcacgtt
    51181 attcaaaccg aaatgggcat cactggtaca accgccaagg aagcgagtga gacgataagt
    51241 ggttcgattg acggtatgaa gtctgcaatc tcaaacttga tggctggact tggtaacagc
    51301 agcgctgaca ttggtatgtt gattggcaat gtggtggaac aatttcaaaa cgtcatcaag
    51361 aatattctac cggtcattca aaatatcgtg tcggctttac cggcagtggt ggatgccatt
    51421 attcaagcat tggggcagtt attgccaaca ctcttagaag cagtaacctc attattctca
    51481 caagtgcttg aaacgatact tggcttactt cccacactta ttccagtggt ggttcaagcc
    51541 gttttaacga ttgttcaaac acttattgag aacttacctt tactgattga cgctgccttt
    51601 caacttgtga caacacttat ccaaggacta ggcgaagcgc tgccagaact tattccaatt
    51661 acagtacagg caatcataac gattgtgaac ggcttgattg aaaacttgcc attactgttg
    51721 gatgctgctt tacaacttat catgggactt gcccaaggac ttatcacagc acttccaata
    51781 ctgattgaaa gtctgccaca gattattaca ggaatcgtga actttattat cagtgctatt
    51841 ccacaaatca ttcagactgg tattcaactt ttgacatcac ttgtgacagc tttgccggat
    51901 atcatcaatg cgattgttgc agctattcca gtgattattc aaaatatcat caatgccgtg
    51961 attggggcaa tccctcagtt gattgacgca ggtattcagt tacttgtggc attgattggt
    52021 gcgttaccgc aaattatcac aacgattgcg aatgccatgc cgcaaattat caacggaata
    52081 acaggaactt tggtaaacaa catcgacaaa atcatcaatg ctggtgtgaa gttgcttgtg
    52141 gcattagttc aaaacttacc agcaatcatt tctgccattg tcaaagcagt accgcaaatc
    52201 atcacagggc ttgcgaatgc tttcggtgag tatgtttcaa ctatgggaac tatcgggaag
    52261 aacttgatta caggattgtg gaatggtatc gctgatgctg gaagttggtt acgcaacaag
    52321 atttcaggat tctttggcgg tgtagtagac aacatcaagg acttctttgg tattcattca
    52381 ccatcaacac tgtttcgtga tgaaatcggg aagtacatgg cacaagggat tggcgtaggt
    52441 tttgataagg aaatgactgg tgtttctaaa acgatgcaga aagctattcc aacaaaactt
    52501 tatatgccaa gctttgatat tgataccgat atccacgctg ccgttgatag cacattctcg
    52561 atgcttgatt taggtgctaa ggttgatgcc ttaacaaaca tcacgagtga aatgttgcca
    52621 acgctactta aagcaatgga cattaaagtc gtcttgaatg atggaacatt ggtcggcaaa
    52681 cttgctccag aaattgataa gagcttggcg acgttgaatc gtagacgtgt agcatattag
    52741 tgttggattt tcacattttg actgtatact ataactaacg agtgtttact gaagagatgg
    52801 tggtaatcat atgttgaagc ataaatttaa agcaaagaaa cctgcactga taggatatat
    52861 tctactgctt gctattggat tatttttaac ggcagtaaga tggtatagcg gcataaatca
    52921 tggttttgta ttcatcaaca atcagattaa ttctcacatt tcaaacttcg cattaagctt
    52981 tcttttctat ttggtgatag gctatctttg gttgcttatg ggaacgaatt taagggttct
    53041 tactgcgatg ggagctggtc tgattgttgc aaatattgta tgtgaaacga tgatgacttt
    53101 tctgaataca ccagatctaa tcgatgcaat atatggaatt tccggcgtgc tggttgtata
    53161 cgcgtattta gcgattacca agaaaatagg gctggaagaa acaaccgaat aggatgatag
    53221 attataaact ttaggattag acattgttgt ctggtccttt tttagttatt tagatgggcg
    53281 gtggtcaatt gaacgcattt attttagacg gaacaattaa ttcacggact gatttaggtt
    53341 tgcgaattac ggaagtgcca gttattccag tgacggaacg tgtcattgat agtattgaga
    53401 ttgatggacg tgaaggagat ttgaccattc tgaaaggctg gaaagatgtg actttcggtt
    53461 ttaaggtagt gatttggaaa tctgatgttt ggactagctg gcagataatt ttgccacaaa
    53521 tcctaaacgc taaaactatc gcattttcaa atgaaccaaa cgtgttttac ccaatcaagt
    53581 atgctaaggc gagcggacta actcagattt tatcttccat gtgggagttt gaaatggagt
    53641 taacctgttc gccttttcgt tataagacca atgtagcgac tatcaatcga accacgtcag
    53701 gaacggtcaa taatcccggc aacatttact cattgccgaa gattaaggtc tacggcaatg
    53761 gaactaggac actcacgata aatggcaagc ctatcgtgtt aaatctgacg aatggcaacc
    53821 tgacactaga ttcggatttg aaagaatgct tttacgggga tgtggcagcc aatcaataca
    53881 tgacaggtga ctttcctgag tttcgagtcg gcagtaatac tgtgactctt ggtacaggta
    53941 taaccaaagt agaaatagaa ccgaggtggc gatacttatg attacgcttt acaacaaaac
    54001 agaatcaaat ttcaatcata acggtctcgc tgtccttgat ggcaacattc tgaaccccgt
    54061 ggtgagtgag gaattgaacg gactcttttc ctttgaattc gattatccaa ttcatgctcc
    54121 acacagtgag gaattgttgc cagaaatgat tgtgaaagca ccagtaccag aactgtccga
    54181 ccagcttttc aggattgttg aacgagatga tgcacttggt ggttttttgc acatagtagc
    54241 tcatcatatt ttctatgacc tagccacaaa ctttattgaa gatacttata tcgtcaacaa
    54301 gaacgggtcg ggagctttga cacaactgct tggtgcaact caattcatgc accgttttac
    54361 cggtacatca aacatcagtg cggtcaataa tgtccgcttg gtgcgactaa atcctgtaga
    54421 ggtgttgctt gatagtgacc tagagaatgg ttttcaaagc agttatggtg gtgaaattgt
    54481 acgcgataac ttcacgattt caatgttaac gactcgtggc agtaacaacg gcgtacaaat
    54541 ccgagacaag aagaatctga caggatataa agctgatgtt gattactctg gaattgttac
    54601 tcgcattatg ccacaagggt atgatggact atttcttccc gaaaaatacg tggacagtcc
    54661 aaacatcaac caatacgtcc agcctaaaat caaagtcatc aagtacgaca acgtcaaagt
    54721 tggtgatgaa gaggatgagt ttactcaagc cgaggcctat gatgaacttc gcagattggc
    54781 tgggttagaa tactcagcta acaaggtcga tttgccaaaa gccacctatg atgtggagtt
    54841 tgccccgctt gagcgaactg aagaatataa agattttgca cagcttgaaa cgattaattt
    54901 gggtgatact gtttcagtta ttcatgccga agatggattg aacatcacag ctagaatggt
    54961 ctcatacaaa tacgatccat ttctgaaagc gtatatctca gtaacactcg gaaacatcac
    55021 gccaaaattc acggatgtgg cgaaggacat caaccgggtg gacaacaagg cggagcaagc
    55081 caaagacgat gccaattacg cattgagttc ggcaaatggt aaaaacacca acttctatgg
    55141 cccgacaact ccaaataatc cgaaactcgg tgacgtttgg tacaaagaaa atggtgacaa
    55201 gtttgaaatg tgggtgtatg aaatccgtga tggcgtcact cagtggtatc cacttatgac
    55261 agatatgacg gcagaagaag taaagcaagc cgtggaacaa gcacaaaagg atgccaatac
    55321 tgccactgaa aaggccaatc aagcatttaa cgatgctgtg aatgctttga atgcagctga
    55381 aagtgtaggt gcaagtgttg aaacgcttac tacatccgtt gaacagaata ctagcaagat
    55441 ttcattagtg gcgagtggta tgtcaactct tgaaggacga gtaatcacag ctgaatcaac
    55501 tctgacagtg caagctgggc agattgctag taaggcaagt caatcgacgg ttgataatct
    55561 gacagggcgt attacaactg cagaaagcac catcacccaa aatgctgata gatttgagct
    55621 atctctttct aaaacaaata aagcggtcaa tgttttagcc ggtatgagca ttgcagattt
    55681 gcagtgggca gttggtggtc tgaatacaaa tgatggaaaa gaaaatacct atggtggata
    55741 cgttcgctca ggttttacaa gagttagaaa tggcgaaaag tatctacttc aaacatttgt
    55801 gggtgggaaa ctttactcta cttacgcaac ggcttatctg ctttattaca aagaggataa
    55861 gtccttttta tcgtacaccc aatttggaaa caccaccaca ccgcttactg ttccaagcag
    55921 tgccgctttt attcgcatt
//
LOCUS       DAYYHS010000010        55939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000010 DAYYHS010000000
VERSION     DAYYHS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.010"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..585)
                     /locus_tag="ACHA6A_000593"
     CDS             complement(40..585)
                     /locus_tag="ACHA6A_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8968826.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(597..1289)
                     /locus_tag="ACHA6A_000594"
     CDS             complement(597..1289)
                     /locus_tag="ACHA6A_000594"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8968827.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1308..1592)
                     /gene="macP"
                     /locus_tag="ACHA6A_000595"
     CDS             complement(1308..1592)
                     /gene="macP"
                     /locus_tag="ACHA6A_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8968828.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1605..2168)
                     /locus_tag="ACHA6A_000596"
     CDS             complement(1605..2168)
                     /locus_tag="ACHA6A_000596"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8968829.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2396..3058
                     /locus_tag="ACHA6A_000597"
     CDS             2396..3058
                     /locus_tag="ACHA6A_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8968830.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3182..4378
                     /locus_tag="ACHA6A_000598"
     CDS             3182..4378
                     /locus_tag="ACHA6A_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8968831.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4479..5855)
                     /locus_tag="ACHA6A_000599"
     CDS             complement(4479..5855)
                     /locus_tag="ACHA6A_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8968832.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5892..6759)
                     /locus_tag="ACHA6A_000600"
                     /pseudo
     CDS             complement(5892..6759)
                     /locus_tag="ACHA6A_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6848..7873)
                     /locus_tag="ACHA6A_000601"
     CDS             complement(6848..7873)
                     /locus_tag="ACHA6A_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8968833.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7891..8565)
                     /locus_tag="ACHA6A_000602"
     CDS             complement(7891..8565)
                     /locus_tag="ACHA6A_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8968834.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8749..9642)
                     /locus_tag="ACHA6A_000603"
     CDS             complement(8749..9642)
                     /locus_tag="ACHA6A_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8968835.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9716..11413)
                     /locus_tag="ACHA6A_000604"
     CDS             complement(9716..11413)
                     /locus_tag="ACHA6A_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8968836.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11406..12719)
                     /locus_tag="ACHA6A_000605"
     CDS             complement(11406..12719)
                     /locus_tag="ACHA6A_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8968837.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12719..16660)
                     /locus_tag="ACHA6A_000606"
     CDS             complement(12719..16660)
                     /locus_tag="ACHA6A_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8968838.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16769..17227)
                     /locus_tag="ACHA6A_000607"
     CDS             complement(16769..17227)
                     /locus_tag="ACHA6A_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8968839.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17525..18043)
                     /locus_tag="ACHA6A_000608"
     CDS             complement(17525..18043)
                     /locus_tag="ACHA6A_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8968840.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18045..18662)
                     /locus_tag="ACHA6A_000609"
     CDS             complement(18045..18662)
                     /locus_tag="ACHA6A_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8968841.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18659..19513)
                     /locus_tag="ACHA6A_000610"
     CDS             complement(18659..19513)
                     /locus_tag="ACHA6A_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8968842.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19510..20421)
                     /locus_tag="ACHA6A_000611"
     CDS             complement(19510..20421)
                     /locus_tag="ACHA6A_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8968843.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20462..21796)
                     /locus_tag="ACHA6A_000612"
     CDS             complement(20462..21796)
                     /locus_tag="ACHA6A_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8968844.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22004..22834
                     /locus_tag="ACHA6A_000613"
     CDS             22004..22834
                     /locus_tag="ACHA6A_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8968845.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23078..23327)
                     /locus_tag="ACHA6A_000614"
                     /pseudo
     CDS             complement(23078..23327)
                     /locus_tag="ACHA6A_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23518..23712)
                     /locus_tag="ACHA6A_000615"
     CDS             complement(23518..23712)
                     /locus_tag="ACHA6A_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968846.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24217..24402)
                     /locus_tag="ACHA6A_000616"
     CDS             complement(24217..24402)
                     /locus_tag="ACHA6A_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968847.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25252..25593)
                     /locus_tag="ACHA6A_000617"
     CDS             complement(25252..25593)
                     /locus_tag="ACHA6A_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8968848.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25593..25823)
                     /locus_tag="ACHA6A_000618"
     CDS             complement(25593..25823)
                     /locus_tag="ACHA6A_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8968849.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26110..26448
                     /locus_tag="ACHA6A_000619"
     CDS             26110..26448
                     /locus_tag="ACHA6A_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968850.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26653..27215)
                     /locus_tag="ACHA6A_000620"
                     /pseudo
     CDS             complement(26653..27215)
                     /locus_tag="ACHA6A_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27947..28528)
                     /locus_tag="ACHA6A_000621"
     CDS             complement(27947..28528)
                     /locus_tag="ACHA6A_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8968851.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28711..29337)
                     /locus_tag="ACHA6A_000622"
     CDS             complement(28711..29337)
                     /locus_tag="ACHA6A_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8968852.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29359..30690)
                     /locus_tag="ACHA6A_000623"
     CDS             complement(29359..30690)
                     /locus_tag="ACHA6A_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8968853.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30845..31246)
                     /locus_tag="ACHA6A_000624"
     CDS             complement(30845..31246)
                     /locus_tag="ACHA6A_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8968854.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31332..31538)
                     /locus_tag="ACHA6A_000625"
     CDS             complement(31332..31538)
                     /locus_tag="ACHA6A_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968855.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31633..33135)
                     /locus_tag="ACHA6A_000626"
     CDS             complement(31633..33135)
                     /locus_tag="ACHA6A_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8968856.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33153..33608)
                     /gene="argR"
                     /locus_tag="ACHA6A_000627"
     CDS             complement(33153..33608)
                     /gene="argR"
                     /locus_tag="ACHA6A_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8968857.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33706..34323)
                     /locus_tag="ACHA6A_000628"
     CDS             complement(33706..34323)
                     /locus_tag="ACHA6A_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8968858.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34544..35917
                     /locus_tag="ACHA6A_000629"
     CDS             34544..35917
                     /locus_tag="ACHA6A_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8968859.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35992..37104
                     /locus_tag="ACHA6A_000630"
     CDS             35992..37104
                     /locus_tag="ACHA6A_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8968860.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37176..38813)
                     /locus_tag="ACHA6A_000631"
     CDS             complement(37176..38813)
                     /locus_tag="ACHA6A_000631"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8968861.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38981..39589)
                     /locus_tag="ACHA6A_000632"
     CDS             complement(38981..39589)
                     /locus_tag="ACHA6A_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8968862.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(39973..41124)
                     /gene="queG"
                     /locus_tag="ACHA6A_000633"
     CDS             complement(39973..41124)
                     /gene="queG"
                     /locus_tag="ACHA6A_000633"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8968863.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(41143..41895)
                     /locus_tag="ACHA6A_000634"
     CDS             complement(41143..41895)
                     /locus_tag="ACHA6A_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968864.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(42036..42985)
                     /locus_tag="ACHA6A_000635"
                     /pseudo
     CDS             complement(42036..42985)
                     /locus_tag="ACHA6A_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(42986..43825)
                     /locus_tag="ACHA6A_000636"
     CDS             complement(42986..43825)
                     /locus_tag="ACHA6A_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8968865.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(43822..44568)
                     /locus_tag="ACHA6A_000637"
     CDS             complement(43822..44568)
                     /locus_tag="ACHA6A_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8968866.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            44806..46176
                     /locus_tag="ACHA6A_000638"
     CDS             44806..46176
                     /locus_tag="ACHA6A_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8968867.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(46231..47127)
                     /locus_tag="ACHA6A_000639"
     CDS             complement(46231..47127)
                     /locus_tag="ACHA6A_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8968868.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(47241..47411)
                     /locus_tag="ACHA6A_000640"
     CDS             complement(47241..47411)
                     /locus_tag="ACHA6A_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968869.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(47408..48085)
                     /locus_tag="ACHA6A_000641"
     CDS             complement(47408..48085)
                     /locus_tag="ACHA6A_000641"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8968870.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(48176..49081)
                     /locus_tag="ACHA6A_000642"
     CDS             complement(48176..49081)
                     /locus_tag="ACHA6A_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8968871.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            49256..50680
                     /locus_tag="ACHA6A_000643"
     CDS             49256..50680
                     /locus_tag="ACHA6A_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8968872.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            50726..51757
                     /locus_tag="ACHA6A_000644"
     CDS             50726..51757
                     /locus_tag="ACHA6A_000644"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8968873.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            51764..52951
                     /locus_tag="ACHA6A_000645"
     CDS             51764..52951
                     /locus_tag="ACHA6A_000645"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8968874.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            53107..54489
                     /locus_tag="ACHA6A_000646"
     CDS             53107..54489
                     /locus_tag="ACHA6A_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8968875.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            54526..54846
                     /locus_tag="ACHA6A_000647"
     CDS             54526..54846
                     /locus_tag="ACHA6A_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968876.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(55046..55714)
                     /locus_tag="ACHA6A_000648"
     CDS             complement(55046..55714)
                     /locus_tag="ACHA6A_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8968877.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        16095 a        11695 c         9912 g        18237 t
ORIGIN      
        1 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
       61 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacgga
      121 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
      181 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
      241 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
      301 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
      361 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
      421 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
      481 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
      541 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
      601 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
      661 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
      721 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
      781 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
      841 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
      901 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
      961 cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
     1021 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
     1081 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
     1141 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
     1201 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
     1261 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
     1321 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
     1381 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
     1441 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
     1501 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
     1561 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
     1621 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
     1681 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
     1741 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
     1801 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
     1861 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
     1921 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
     1981 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat
     2041 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
     2101 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
     2161 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
     2221 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
     2281 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
     2341 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
     2401 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
     2461 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
     2521 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
     2581 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
     2641 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
     2701 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
     2761 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
     2821 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
     2881 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
     2941 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
     3001 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
     3061 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
     3121 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
     3181 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
     3241 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
     3301 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
     3361 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
     3421 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
     3481 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
     3541 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
     3601 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
     3661 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
     3721 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
     3781 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
     3841 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
     3901 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
     3961 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
     4021 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
     4081 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
     4141 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
     4201 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
     4261 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
     4321 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
     4381 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
     4441 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
     4501 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
     4561 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaaaat ggagacgatt
     4621 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
     4681 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
     4741 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
     4801 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
     4861 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
     4921 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
     4981 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
     5041 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
     5101 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
     5161 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
     5221 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
     5281 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
     5341 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
     5401 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
     5461 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
     5521 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
     5581 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
     5641 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
     5701 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
     5761 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
     5821 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
     5881 gaaaacagcg attaaactgt tttcatatac cctaaaaggg ataccaccta aaatgactaa
     5941 aaggcaacca acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa
     6001 aatcccacct aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa
     6061 accaagtgat ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca
     6121 gatcatgttt ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc
     6181 gcctaagatc attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca
     6241 gttgacgatg atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc
     6301 cttaccaaaa tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc
     6361 agtcaaatac gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt
     6421 cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa
     6481 accagtagac aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc
     6541 attttgtcca acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt
     6601 gattacagga ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat
     6661 tcctagtgtt tgttgattgg catcgccacc aattttacca cttactaaac caatactacc
     6721 ccatgccaac atagggacta atgctattaa aatttccata accaatctcc ttttatttca
     6781 catgataaga aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa
     6841 cgattttcta acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg
     6901 tcccttttcc ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt
     6961 ttgctaaata aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag
     7021 taaatccctt atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt
     7081 taactgaaag gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat
     7141 ccggcgtata tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag
     7201 catcggtcaa aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa
     7261 aataatttgc ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga
     7321 tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt
     7381 catcgatttt tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact
     7441 cgatcgaacg aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg
     7501 agtcaatata atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt
     7561 gttcacggat cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac
     7621 cactcaaata attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc
     7681 ttctcttgct caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag
     7741 ctaaataagc aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga
     7801 tcgtcaatgc aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt
     7861 taaaaaaatt catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt
     7921 tgtttcgata tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt
     7981 gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt
     8041 ttcccgactt aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt
     8101 tttgctcaaa tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt
     8161 aatgccattg tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag
     8221 taatgcattg atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc
     8281 tgcccccatg ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat
     8341 gatcggtact ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg
     8401 aagattaata tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg
     8461 attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa
     8521 ttgtcgaatg atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat
     8581 ctttcttctt gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga
     8641 aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc
     8701 tcttccaagc agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt
     8761 tttctttcgt tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag
     8821 cactagcaat atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg
     8881 ttctatcttt gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg
     8941 gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct
     9001 aattgctaat gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc
     9061 gagcatccga ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc
     9121 cgtaaagagt tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc
     9181 tcctccagtt ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc
     9241 attcgtttca tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat
     9301 catatgttgt tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa
     9361 aggatctttg acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt
     9421 cttttcatgc actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt
     9481 ttgataattc tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg
     9541 atatgtaaaa actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag
     9601 actgatttgg gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt
     9661 taaaaaaact gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata
     9721 cttcagactt cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc
     9781 cgattagtaa gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca
     9841 tattcggtac tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat
     9901 agcttccata agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac
     9961 cttctggcgc catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa
    10021 agtatccatt tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc
    10081 tatctgctcc tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt
    10141 cagaacggtc ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg
    10201 catcatattt ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt
    10261 cttcataatc aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat
    10321 catccgcttc tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac
    10381 gtgcatctgc taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa
    10441 tcgtcacgac aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa
    10501 tcgtctcgcc ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg
    10561 tatctgtgat tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga
    10621 aataaggaat aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat
    10681 aagaaatcgt ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt
    10741 cctctacatt catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat
    10801 ctgtattgta tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca
    10861 tcatcactgg catcataaca accattggag acgcgattct ggaaatatga gctggcgaat
    10921 aagtttctaa gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga
    10981 cggtagagaa taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc
    11041 gatattttaa aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag
    11101 aattaagacc gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata
    11161 cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg
    11221 tagaaaccaa tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta
    11281 atgtaatcgt cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt
    11341 tcccgaaaat ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca
    11401 tttttttatt catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat
    11461 aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac
    11521 cattttcctg attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt
    11581 tcttttacgg tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact
    11641 ttcccggatt ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct
    11701 tttgcatcca cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc
    11761 tcgtaacctt ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt
    11821 tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc
    11881 aaaggatcat tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata
    11941 tctaaataat atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat
    12001 ggttttcctt tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt
    12061 agataaccga catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc
    12121 gggtgataaa ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc
    12181 aatccaactt ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg
    12241 tttttcttag gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc
    12301 ccagttgtta ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata
    12361 ttgtttctgt tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca
    12421 gctttttttt catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt
    12481 ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca
    12541 tcattttcat ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc
    12601 aacacgaatt ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg
    12661 actgctgcaa gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt
    12721 accttgctcc ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg
    12781 ccatgaagaa tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca
    12841 acggattttc aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa
    12901 gttcatattc taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg
    12961 aaatctcaac ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt
    13021 caggttcttc tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt
    13081 caggtgactt cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata
    13141 gggaagaatc gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac
    13201 tattttgttt ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa
    13261 ttttcaaaag ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac
    13321 catcgatgat ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt
    13381 caatgataaa cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat
    13441 acccatttgg tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt
    13501 ggtcgtaatt taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga
    13561 tcccaggcaa acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca
    13621 gcgggaagga agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc
    13681 cttcatggtc taattttcta actcggatct cccgataatc aaacgtattg taagcagtga
    13741 atgctaccca taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt
    13801 cacctgtttg cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg
    13861 tgtcattttc atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt
    13921 ctgcatagtt ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg
    13981 gatgaataac tttctcacca tccacgatta catatttccc gtcttcatct actgaataga
    14041 acgtgagtct ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg
    14101 gatatccgat ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga
    14161 ttggcatatt actgccttca attagtttat tgatattcat gtggattggc ttgaattcat
    14221 ttcgcatttc cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt
    14281 gtacaacaga aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg
    14341 gtccgccgac tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct
    14401 catcttcgtt ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat
    14461 aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat
    14521 ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg
    14581 tttcgattgg ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc
    14641 cagccaacgg ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt
    14701 cgtaacgaat ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat
    14761 cctccaggtt ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc
    14821 ccgcatcaag ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg
    14881 gagaaaattt ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt
    14941 ctggtggggc ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc
    15001 cgttttctcc tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg
    15061 gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg
    15121 tagaagagat atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc
    15181 gcggtgttac gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag
    15241 catctagtcg ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac
    15301 ttggcgggtt ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg
    15361 cagatgactt ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac
    15421 caacccagtg agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat
    15481 ttacttcatc ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg
    15541 cattagaagt attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag
    15601 caatcttatc ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac
    15661 ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt
    15721 tttctggatt tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa
    15781 gccctagtgt aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc
    15841 ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt
    15901 tattgatagc tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg
    15961 ggtcagagag cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag
    16021 tttgactatt ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa
    16081 cgatatcgat tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt
    16141 aaagggttgt tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt
    16201 ctacccattt tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt
    16261 catttcccca aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg
    16321 atccattatt agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac
    16381 tatcatcgat caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat
    16441 tgctttgtgt ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc
    16501 gatgctgttc gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag
    16561 ctgttggccc aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg
    16621 cttgaccgct gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt
    16681 gaatttcagc ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc
    16741 tttcatcaac caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg
    16801 tttcaaaaat caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt
    16861 cttgaccatc gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac
    16921 ctgttccacg aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac
    16981 gtattctacc ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt
    17041 cttctttttc aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat
    17101 cctcctttgt ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact
    17161 ggaaatatgg tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc
    17221 ctttcacatc cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa
    17281 caatgcccat ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt
    17341 tagttaaaaa acaccaatta tacaacattt attattaaaa ttaaattata aaattttata
    17401 acgttctcat ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc
    17461 tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat
    17521 taattcagat aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta
    17581 aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa
    17641 ataaaaaggc taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa
    17701 aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga
    17761 caagccgacg atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca
    17821 aaccaacaaa aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat
    17881 caggtatcaa gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat
    17941 tactgataaa tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact
    18001 tgttgataat actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta
    18061 agaacctttt cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg
    18121 aaacctaata aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac
    18181 gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta
    18241 atccaagaag cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg
    18301 cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa
    18361 tagcggaaaa tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct
    18421 tgctttccat aaagagagaa gttctcacga aactttttcc agaatagtga accgagcttt
    18481 ttctcttcat tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct
    18541 aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga
    18601 atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc
    18661 atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga
    18721 acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg
    18781 tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt
    18841 ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa
    18901 tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat
    18961 tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac
    19021 gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag
    19081 tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg
    19141 ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct
    19201 tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag
    19261 tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat
    19321 agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca
    19381 ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta
    19441 ctgtcatgag gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga
    19501 aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa
    19561 attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata
    19621 acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga
    19681 atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa
    19741 agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt
    19801 aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga
    19861 tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat
    19921 tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg
    19981 gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat
    20041 ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa
    20101 tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt
    20161 cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa
    20221 atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt
    20281 caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac
    20341 caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt
    20401 tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg
    20461 attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt
    20521 ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat
    20581 ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt
    20641 gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg
    20701 tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag
    20761 aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca
    20821 agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca
    20881 tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat
    20941 tttgggcacg catggctact aatccggaaa taaatggatc agatacacca gacccaaatt
    21001 cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact
    21061 ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac
    21121 tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa
    21181 aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta
    21241 attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt
    21301 tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga tcaccttcat
    21361 attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt
    21421 tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta
    21481 caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac
    21541 cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa
    21601 tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat
    21661 tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg
    21721 cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa
    21781 acaataattt tttcatatct tacctccaaa ttttaatgta accgatgccg gttttaatat
    21841 aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt
    21901 aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt
    21961 agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa
    22021 agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca
    22081 ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg
    22141 ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga
    22201 aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt
    22261 tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat
    22321 gaatccacag cacgcgatcg tagatggctt aggacctcgt cctgctgaca gcgtgggcaa
    22381 tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa
    22441 tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga
    22501 tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct
    22561 atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac
    22621 tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg
    22681 agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata
    22741 tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc
    22801 aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt
    22861 tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca
    22921 aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg
    22981 gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag
    23041 gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat
    23101 ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt
    23161 gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt
    23221 ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt
    23281 atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg
    23341 gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc
    23401 tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag
    23461 aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta
    23521 tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata
    23581 aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact
    23641 agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag
    23701 tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt
    23761 actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc
    23821 aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat
    23881 ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata cataaccttg
    23941 atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct
    24001 aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa
    24061 gtatctgaac tattaatttt taaactatca tttttatcct caactacatg cacatctgtt
    24121 gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct
    24181 tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc
    24241 aacagcaaaa aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga
    24301 aaaattcagc aactcattac tgtatgattt tagcagccca accaacatat accctataaa
    24361 agctaataag atggttagga atcctttaga atttttactc attcatagac acttccttaa
    24421 ataactttta ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata
    24481 aaaaaaacct tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact
    24541 gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta
    24601 gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac
    24661 tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta
    24721 gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga
    24781 tgttccagtg atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt
    24841 tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta
    24901 cgtttttgag catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt
    24961 tttaggacta aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact
    25021 tttcgtatac gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg
    25081 acttgaggaa atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata
    25141 cggaaattaa tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt
    25201 aatttcgtta tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat
    25261 atactgtata atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt
    25321 gagttttcgt tccttaaaat caagagattt taattgtgta ataattactt gtccttttat
    25381 atctagctct tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg
    25441 acatatgacc gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg
    25501 tcgtcttttt tgaatttctt ttcctacgga gggatcaaaa tcaatccata caatatcacc
    25561 ttttttagga atataaattc tttcttctga cactataaaa tttctctccc ttcaggagtt
    25621 aaatcttccc attcgtcttt ctcataatac tcggcctctt ccccaccact aaatggatct
    25681 tcaatttttg gaactaacgt aattgttcca tcatcagaat acacaacgat atattcttgg
    25741 ttttctgaag gtttctttcc gttatcagaa ggtaaagtta ccactacaga acttccttgt
    25801 aatctagatt ttgtaacaag cattatcatc actcccttaa taaaagtata ctatataaca
    25861 ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt ttaaagtata
    25921 tcttattgga tataaataga aataaattgt agccagttca ttttttcttt aactaaaaaa
    25981 ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta tagctaaccc
    26041 cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa gacaacaggc
    26101 catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc tgacaaattt
    26161 caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg gatcaaagaa
    26221 tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga ctttgctatt
    26281 gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga tccaactcat
    26341 atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac aactgccaac
    26401 cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg ataaacatcg
    26461 ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag ctatacatta
    26521 gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagcat ttcttctgtt
    26581 ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc tttctttgaa
    26641 aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag taatgatttt
    26701 tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca agacaagcgg
    26761 cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta tgattgattc
    26821 tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta ttttttcctt
    26881 atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac acaacgattt
    26941 tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg ggcattaatc
    27001 atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg agtgaggatg
    27061 ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc tccagctgct
    27121 aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt ctgaaggagc
    27181 tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa tctttttttc
    27241 tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt tgtagaacta
    27301 aaaaagttgt gacaagtgac aaactctcca gtgaatggta ttctcctgcc aactcctcgg
    27361 tcacaagtca caatattcaa caaacatgag aagctgcttt ttgaaattgt tccttgtgac
    27421 tcggagtgaa acggcaggct tacgacctct gggtaaacgg tgttcaaaag ccagtttctc
    27481 ggtattaagc caaatttatg caaaaatgta gaaagctatt ttcacaaaat ttttcttaat
    27541 actcgaaact aagcgacttt ttcacaacct cttccttaaa cggtgttcaa aagcttgcat
    27601 ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc tatttttatt tggctgttct
    27661 tattacttga tgcagggcag ctttttcaca acctctccac taaacggtgt tcaatcagct
    27721 aaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc
    27781 tctaatactc aggtcaaagc gctgatttca caacctctaa tacacggtgt tccagaagta
    27841 gcttcttcga aaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc
    27901 cttcttattt cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt
    27961 gcgaagttaa caatctttct ttgatcaatt gaaaattctg cctaagtagt gatagatcct
    28021 gtgtgacttt caacaataag atacttccct ggatttcagc aatcaacgct ttggcttgac
    28081 gatctgcttc ttcatcggac agtttttctt cagaaagtgc caggatattt tgtttcgtat
    28141 acttgaccca ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac
    28201 tcaactcaat aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat
    28261 cagcgaaaat ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta
    28321 agatgtcttc gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac
    28381 aaacttcttt ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg
    28441 ctagaatatc tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg
    28501 cttccaagat cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct
    28561 atattataca gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa
    28621 ttatctgaca cagccctcac tataaaactg ttctcatata gctagatgtt cattgattca
    28681 agaactgcga ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt
    28741 tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg
    28801 gccaggagca ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc
    28861 acgcatatgg gaatcgctac acaagtttcc gttcattcca ccaagaatta ctgtgtcgat
    28921 tccatttttt ctcaattgga gcgccaaatc attgctttct ggtccaaaga ttttatgagg
    28981 agagctgatg atcgtatttt catccaaata aggtttcaat tcttcgataa aatcggctcc
    29041 tgaaccttct tccactgatt catattgact ttttctagca aacatctgct gctcaagcat
    29101 catatgttct cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg
    29161 agaaataaag agtttgtatc ctttttcttt tgcttgtttt accatttcaa tcatattttc
    29221 gatcgtattg tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt
    29281 caagaactcg acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt
    29341 ggattctcca ttcgttattt attttaatac atcatttaga gcttcactca ttgtcggatg
    29401 agaatagatt tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat
    29461 caagttgatg acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt
    29521 ttcagggtcg actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg
    29581 gacaccgctt gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt
    29641 ttctgttaaa cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt
    29701 ccggttattc gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc
    29761 atctagagag atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt
    29821 gtctgcatct gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac
    29881 atctgtattt tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac
    29941 ttttgacgct tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga
    30001 ttcttggtct gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt
    30061 catgtcttct aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa attcagaatg
    30121 ggcatcaaga acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc
    30181 aatatatcca gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg
    30241 cgtagaatca atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc
    30301 agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac cattattcgt
    30361 cacttcgatt tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc
    30421 ctcatcgctg atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg
    30481 agcaacagcc tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg
    30541 aatacagccg atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa
    30601 aaccgattca ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc
    30661 gatcacaata ttttgatatt tttccatcat ttttccctcg cttctttttt acctaccgat
    30721 tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc
    30781 aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat
    30841 tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc
    30901 agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat
    30961 gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa agagagacac
    31021 gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc
    31081 cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat
    31141 ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat
    31201 tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt
    31261 cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac
    31321 aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa
    31381 aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct
    31441 acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa
    31501 accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct
    31561 ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc
    31621 aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt
    31681 tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac
    31741 tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc
    31801 tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc
    31861 caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa
    31921 caattttaat cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc
    31981 taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata
    32041 tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc
    32101 tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata
    32161 gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga
    32221 agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact
    32281 taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga
    32341 acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg
    32401 cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc
    32461 atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca atactttttc
    32521 agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg
    32581 tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg
    32641 tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg
    32701 tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat
    32761 tccattataa taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa
    32821 ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac
    32881 atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat
    32941 cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt
    33001 tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt
    33061 tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc
    33121 tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct
    33181 gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc
    33241 atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca
    33301 ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg
    33361 cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat
    33421 gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct
    33481 cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct
    33541 tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc
    33601 tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca
    33661 tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat
    33721 tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta
    33781 aatctctttg tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa
    33841 tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag
    33901 accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga
    33961 acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa
    34021 ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc
    34081 ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca gccaattttt
    34141 tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa
    34201 gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt taggaacctc
    34261 tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc
    34321 catttttctt cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg
    34381 acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa
    34441 atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag
    34501 aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca
    34561 gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat
    34621 cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc
    34681 acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc
    34741 tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt
    34801 cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg
    34861 attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac
    34921 atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat
    34981 tgctccagca attggcccca cttatggagg cattatgact tcacatttta catggaatca
    35041 tatcttcctg tttcttgtgc ccgttttgtt tctttctttg attatcggct ggtttgctat
    35101 tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg
    35161 tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat
    35221 tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga
    35281 gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg
    35341 cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca
    35401 gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt
    35461 tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc
    35521 aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg
    35581 acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt
    35641 atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga
    35701 cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt
    35761 agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac
    35821 attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg
    35881 attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc
    35941 atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa
    36001 attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca
    36061 aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg
    36121 attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac
    36181 ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca
    36241 caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc
    36301 gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag
    36361 acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt
    36421 cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg
    36481 gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga
    36541 ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct
    36601 ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca
    36661 tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct
    36721 gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa
    36781 ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag
    36841 ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt
    36901 gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg
    36961 gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta
    37021 gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc
    37081 tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata
    37141 ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc
    37201 tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat
    37261 cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa
    37321 acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat
    37381 tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt
    37441 ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac
    37501 tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt
    37561 tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt
    37621 gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc
    37681 ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg
    37741 actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct
    37801 cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta
    37861 aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa
    37921 ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag
    37981 atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg
    38041 gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca
    38101 ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct
    38161 ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg gagagcatca
    38221 aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa
    38281 agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct
    38341 ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa
    38401 tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg
    38461 cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg
    38521 tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg
    38581 aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa
    38641 tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt cctatcaaaa
    38701 acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac
    38761 agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct
    38821 cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt
    38881 actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa
    38941 taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta
    39001 accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta
    39061 cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac
    39121 ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc
    39181 attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt
    39241 caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg
    39301 ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg
    39361 ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg
    39421 catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg
    39481 attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag
    39541 caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct
    39601 ttttttctcc gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat
    39661 aggtaaaaga aacatgacat ttaatgacaa aataacgaat tatttaatat ttgtaacatt
    39721 taaacaaata tttttttgat acaaaaccat aaaagccttg ttgtataagc aaagtaatcg
    39781 tttttctatg tttttttatt tttgaatgaa aaacgcagat tctgtaacat tcactgcaat
    39841 aatccataaa aaaaagcaaa aaaatttttg atttttatca aattctctta aaaaaatgta
    39901 aaaaatacaa aaaaaccagt aattgttaca acaattactg gacaaaaact ttgaataatg
    39961 ttaactattt ttttatttta actttctccg caataatttc tgggcatgtt ccaattcgtt
    40021 tataacgact tcttctgttt ctttagctaa tatttcatct aatacatcaa gccatttttc
    40081 cggttctttt gttcctagac gtccaatagc ccaagctgct gttcctctga tgacagggcg
    40141 gacatctgtt aaacattctg aaatctcagg caatgccgtt cgatcaccta gattagctaa
    40201 agcaatcaat gcgtttctct gcaaaggttt cttcccacgc catgaaccag ctaaatgccc
    40261 aaattgctgt ttgaagtctt tattagacat cgaaagcatt ggctttaatt ttggatatat
    40321 ttcgtctact tttggttcca tctcttcatg aaaatgaaaa tcttttcctt gattgtaagg
    40381 gcagactaac tggcagatat cacatccata aatgacattt cgcatttttt tacgatattc
    40441 ttccggcatc atcccttttg tctgagtttg ataagacaaa catcgctggg cattcattct
    40501 tccgtcacca agcaatgctt ttgtcggaca agcagtcacg cagcgcatac agtcaccgca
    40561 accaaattct cctggtttat caggctcaaa tacaatatta gtaacgatct caccaagata
    40621 gacaaatgaa ccgaactctt cagtgatcaa caatccgttt cgtccaataa atcctaaacc
    40681 agctcgctgt gctacagcga catcgatcag ttctccagta tctacttgcg gagctagacg
    40741 ccattcctct tctttttgcc atttttctgc tcttgtttgg atgaattcga tcaatcggtt
    40801 caaacggtct ctgagtatat catggtaatc gatcccccaa gaagcacgag caaactgccc
    40861 gcgtttttca tccctaggca cttcttcatg gatcctagta ggatacgcaa gtgcaatgga
    40921 aatgatcgat ttcggctgat caaatgtcat ttctggataa agacgctcat cgatatttgg
    40981 atgttcaaag ccagaagtat ggccagctgc tttttgttct tccaacgatt cttttaatga
    41041 atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt cctaatcgtt tgctttcttg
    41101 tatgatttct tcttttaatg tcatggtctt cctcgcttgc tgttaacggc tgtataaccc
    41161 ataatatagt ttttcttttt tcagctggac aaactggatc tgctgatcgg caatcttctt
    41221 ttcttccatc catttttttc ttgcagcaaa tacttcccaa taatttgttg aaattaattt
    41281 tcttttgtta tctaatgcat taaaaaaatt caatgaaact ggttttgtac ttaacagttc
    41341 atggagcctt acctcttccc atggcgaaaa ctcagacaga tcgcgggaat acaatacttc
    41401 ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc gggattttcc caggcctagc
    41461 aatttctact agacgattat aaaacatatt cattaatttg ctgttggcaa taaattgact
    41521 tttataatag gccgactgga tctttttgat tactccttct gctttgatga ctgaaaataa
    41581 tgcaggccat gctttgacgg ttatttcgtc gttttgttta actaatacgc caggtgtgaa
    41641 gccgactgga gaaaggaaca caaaataacc gataccggta ttttcatcta aaggctgaaa
    41701 ttcatcaaac aaccatttgc ctatgtacag ctcttcatct ggttctgttg tctcattttg
    41761 atttgctatg aataatttag ctaattctgc ttcaagaaat tgaaggtctt gatccttgtc
    41821 ggtattttcc tgctggctga tcaatcgaaa gacttcttct ttaatcgtat atttatcttt
    41881 acgtgtaaaa ttcatagcca caccttcctt ccttactcta actataacgg aatttcttca
    41941 aaaaaggtat ctgtacgacg aaaaacagca taattttttt tcatatctct tataaaaaat
    42001 gcaaggtctg ctcctcaagc tagaccttgc acttttcagc tttcttttgt ttgtgacaaa
    42061 ccttcatgga tcgtttcaat attccatttc atcatctgat agtaagaatc tccttgttct
    42121 ccttcttttg ctatggaatc agtgaacagt ttatcataga ttttcaaacc cgtttctttt
    42181 gctacccgtt ccatgcttct tgagtctaca ctggtttcca cgaataaaac cggtacttct
    42241 tttgctctta tctgatcaat gatcgctttc atttgatcag gcgtaccttg actttctgta
    42301 ttgatctccc atatataagc tgctggcaaa tcatatgctc gtgaaaaata cttgaatgcc
    42361 ccttcacttg ttactaataa ttttttgttc tcttcgatag aagcaaaact ttccttcgct
    42421 tgtgtatcta attcttttag tttttccaca tatgcttctg cattttttta tagagtgtcg
    42481 cattttctgc atctttttca gagaatatac gtgcgatttc ctccacatat tttattccgt
    42541 tagataggtc tagccaagca tggggatctg cttttgtatg ttcttcaccg ctagttaaat
    42601 aaagaggttc tacatttttg ctaactgcaa aataatcttt tccttcttct tttttagccg
    42661 tttccatcaa gttatcgaac cagctgttac ctgtttcaag attcaaaccg ttgtataaaa
    42721 taacatctgc atcgcttgcc tttttgatgt cttctggtag tacttcatat tcatgcggat
    42781 ctgttccgac tggtacgata ctgtggatat ctatttgatc tgttccgacc tcttttgcca
    42841 tgtccgccaa gatcgaattg gtagccacga ctgacaactt ttctttactt ccttcatccg
    42901 cttggtcaga agcctgcttt ccgcaaccgg ctaataccaa gaaactaact gccaagatta
    42961 aaaagagtga tttttttgtt ttcatctatc tgcttccttt ctgaaataaa attccttttg
    43021 ttggagaaaa taaaaaagca aggataaaaa atgcggtaca tgctaacacg atcgtagctc
    43081 ctgatggcca attgtaacta taactgaaaa acacaccgac aactgcactt agcataccga
    43141 ttcctgcagc tgtaatgatc attttcagta aatggttcgt tagcaagtaa gccgtggcgg
    43201 ctggtgtgat caacatggca atcaccaaga ttgttcctac tgtctgtaaa ctgactacag
    43261 ccactaatgt caaaaagaac atcaaaagat aatggatcag cgatgtgttc agcccataag
    43321 cttttgccat tgtcggatcg aatgatgtga tcttcaattc tttataaaag aaaaagacaa
    43381 atatcaagac gattccacta acgagtgctg tcagcaacaa atcgctttcc ctaacagcta
    43441 agacattccc aaataaaata tgatacaaat ctgtcgcact ctgggcatat gaaatcaaga
    43501 taatccctaa tgcaaaaaag gagctaaaga caattccaat cgctgtatca tttttcaatc
    43561 ggctccgctg agtcacgaaa ccaatcactg ctgctgttag catcccaaaa gcagtggctc
    43621 caaagatgta actaaaacca aacatataag aaatggctac acctggaaga actgcgtgag
    43681 agatagcgtc tcccatcaaa gacatccctc gtaaaataat aaaagaaccg atgactccag
    43741 aagcaagtcc tacaagcatg gaagtgacca aagcgtattg caggaattga taattcatta
    43801 atccatcaat aaaggattgg atcatttttt ctctcctcct tttactaaat gtctgatgat
    43861 ctcaccatag gccaattgaa ggttcttcga tgtaaagact tcttgtactg gaccggaagc
    43921 gatcagctgt ttatttaaaa gaataacctc atcgaaatat tcttctactt tatgaagatc
    43981 atgatggacg atcaaaatcg tttttcctga attcttcaat tcctgcaaga tgtcaaagat
    44041 ctttcgttca cttaacgcat caatccctac gaatggttca tctaaaaaga tccattctgc
    44101 tccttgggct agagctcttg caataaaaac tctctgaagc tgtccacccg atagttcgct
    44161 gatctgtctt ttggcatatt cttccatccc tacttttttc aaagcttgtc ttgcacgttc
    44221 tttttctttt ttcccaggtc tttgtccaat tcgtaaagat ggatatgttc ctaaaagtac
    44281 aacgcctaat acatcaattg gaaaggaaag atccaattca cttcgctgtt ccacgtaggc
    44341 tattttttta cggactgagt ttaccgactg tccagaaaag gtgactttac cagtagtagc
    44401 tggtatcaac tctagcaaag atttcataaa tgttgatttt cctgcaccgt ttggtccgat
    44461 aataccggta attttttgtg gatttatcgt caagttgata gttttcaaaa cagttttccc
    44521 ttgataagcg gctgaaaggt cttgaattac aatggccgta cgttccaaat cttacactcc
    44581 tttttaggtt tacctaactt tttatttaaa tatagtttaa ctaaaatgga aagcattttc
    44641 aagtagaatg atgaaaaagt ttttttcgaa ttttcctcgc attttatgaa aattttctgt
    44701 agaatcacat caagagttca ataaggctaa cctaataatg aaaaacatgt gttgaaacgt
    44761 ttgattattt tttcttgttg actcaacaac gaaaggagtt tttgtatgga agaagaaaag
    44821 ttaactcgaa gtcttagtgc ccgtcatatc caaatgatcg cactaggtgg aacgatcggt
    44881 gtcggtctgt ttatgggtgc ctcctcaacc atacgttgga cagggccctc agtgatggga
    44941 gcctatgcga ttgcaggatt ctttttgtat ttgattatgc gtgctttagg cgaaatgctt
    45001 tacgttgatc cttcgactgg ttcttttgca aattacgcga gcgagtacat ccatcctgtg
    45061 gctggctact tgaccgcttg gagtaatatt tttcaatata ttgtcgtagg aataagtgaa
    45121 gtgattgctg taggatctta tatgaattat tggtggcctg atttgccggc aattattccc
    45181 ggaatcattg tcgtcttgtt tcttatgctg gcaaatctta tttctgtcaa ggcgtttggc
    45241 gaattagaat tttggttttc gatcatcaaa gtcataacga tcattttgat gatcattgca
    45301 ggtcttgggg taatcttatt tggatttggt aatcatggtc aggcagtagg gatttcaaat
    45361 ctttggaaaa atggtggatt cttcactgga ggcgtcaaag gatttttctt tgcattatcg
    45421 atcgttgttg cttcctatca aggaatcgaa ctgatcggaa tgactgctgg tgaagcagag
    45481 aatcctaaaa agacgatcat tgaagctgtc cagtccacta tcggtcgaat cttgattttt
    45541 tatattggtg ctatttttat cattgtgagt atctatccgt ggaatcaatt gagtgagatt
    45601 ggttcacctt ttgtacaaac tttttctaaa gtcggcatta catttgctgc aggactgatt
    45661 aattttgtcg tgattacagc ggcattatca ggttgcaatt cagggatttt cagcgcaagt
    45721 cgtatggtct atacacttgc cataaatgga aagatgtcta aaaaatttct gaaactatct
    45781 cgtcacggtg cgccttttta tcctgtcgta gcaatctcag gtggaatctt aattggtctg
    45841 atcttaaatt atcttctacc aatgttcatc aaccagtcag aaaatatttt cgttttcgta
    45901 tacagctcaa gtattttgcc tggtatggtt ccttgggtag tgattttgat cagcgagatt
    45961 cgcttcaaaa agaagcacgc agataaaatg catgatcatc cttttaaaat gccactatct
    46021 ccttatacga attatgtgac acttgctttt ttagctatcg tattgatttt catgtttatc
    46081 aatcctgaaa cgagaatctc attgattatt ggactcgttt tcttgcttta catgacgatc
    46141 cattactttg tacgcgaaaa aagaaccgga aaataaaaaa agaacgaagc ttcaccttag
    46201 tggaagcttc gttctttttt attgctttta tcaattcaat tttagcgtct caccgggagt
    46261 caggaaataa ttctccatgc tcatcccatt cagcgacaag atcgtttcta ctggtacgcc
    46321 agttcgttcg gctatttgat ttggaccttc tccttccaaa actacggttg tcgcagcctg
    46381 tgaagtcgcg ctgttggtat tagtttcaga ggaatctgtt gtgtcttgtg ttgttcgagc
    46441 ttgcatttca gaagcttcta ccgactctgc tgtctcagaa tcagaagatg ttgtgtcttg
    46501 tgtgcttgtt gactctgctt gaagagaggc gctagtctgc aaataaatat cggaaatctt
    46561 agcgacatga ttcgtataat tagccgataa ttgattgatc aaagtatcta agtctacacc
    46621 gtctttattt tctgctgcct gatacgctgc acgtaatttt ctgatccctt cgtcggagat
    46681 tgcttgaagc tcacgtgctt tttggttagc aactgctgat aaaccagcta ttccctcttg
    46741 atttccctga atttctgtct ggtattcttc aatcaatgca ggcgtctttt cttcgatctt
    46801 tttcgaataa tcatttacga ttgcttgaag ttgctctggc aaaccatttg ctgttttttc
    46861 ttcagaagtt tgtgtctctt ttgttttagt tgatgactca cttgtttgtt tcgtctctgt
    46921 tttctttgtc gttgtttccg ttatggtcgt acttttcgct tctgacgaat tttttccatg
    46981 aaaatataga cccgcaactc ctcctgctat gatcaaaaac acggcaactg ctgccccaat
    47041 catgatcatc cgcttacgag ctgcctgttt ttgctgtctt cttcgggtca tccgaacatt
    47101 ttctccccac tcattttcat catacatttt tttcaccacc aatttttatg tataatagcg
    47161 ttatcaaacg cttcatattc ttattgtagc aaataattgt cgctgtgcac atttttatct
    47221 ttgttattta taagaaaatt tcaccagttg agatgattcc aagattgtcc gtcgatttct
    47281 ccttgcagct gcattgttcg ttcgtctatc tcagccatca gtttctttat ttcatataag
    47341 agcgcacggt cttgatattc tgatttttcg cttaattcat ctaattgttg gatcagccaa
    47401 tcatttgtca taatgaagtt cctttcattt cttgcagtgt aaaggtcaag tcttcgatct
    47461 gctcttttgc ctcagtcagt gcttctacga tatatgcttg tttttcagat tcttcttcct
    47521 gcattttctg attttcctgt actctttgaa taaaccagtc aatccgctga gtcagcgatg
    47581 gtttgccttc caatgagaga tactgctgat aagcaattag cttctcccaa cgtttttcct
    47641 gggataaata cataaattgt gctaaaacga atggcggaag ataggtcata tggaatttat
    47701 ttgcgattcg ctgatacggc ctcaaaaatt cggaaatcgt atattcttct tttcctccag
    47761 cttggtattc tttttccgcc acacctgttg tcaatacgat tcctagttct ttccctttta
    47821 atacatgtgc tgctcgttcc aagacattat cttgccaaat ttttaaatga gcaggagaac
    47881 tataccagta aaacggaaac tggaaaatga tccgatcatg ctgggccaag agctgtagtt
    47941 cagtctctat atctattttt ccgtctggat aacttgattc caaatgatgc caagtagctg
    48001 gtaaaccagc agaagcttct ttgaaaaatt gctgatttaa agattcttcg atcgttggat
    48061 ggctaatgat gataagtgta cgcatccctt ttcctccttt ttagtcttcg ctttacagtg
    48121 tagcataacc gccacaagtt tcttttgccg atgcttgtca ttttaaccag actaatcagg
    48181 ttgtccaaga aactgcggtt tgtttttcca actgtttttt caaggtatcc aaagcaatat
    48241 tcttaaaatt ttcgtttttc atacgtttac gatagttcgt agccgtttct ccggtactct
    48301 ttttgaacat tcggctaaat acgactagac tattgaaccc tacttgatag gcaatacttt
    48361 ccacggattc atccgtcaga gaaagaagaa acgcccctcg attgatccgt accccgatca
    48421 aatattcctt gatactggta cccatatgat ttttgaatac tcgcgacaaa tggctccgat
    48481 tgaccgctaa atgctgggcg atttcctcaa cagacaattt ttcagcataa tgtgcctcga
    48541 tgtatttgac tgtttcaaca gctaaagagg aatgaacctt ttgtacacca ccgctgacct
    48601 ttccgccatc ttttaaaagg actgataaaa aacggtaggt taatgctgtt aactgtaatt
    48661 cattttccaa ggtttcttcc gtatctttca tgcattctaa aataatgtcg atatattttt
    48721 gtatgtcaga agagaccatc gtgtattggt cttctctgaa aagcgactgc cgaatgattt
    48781 cttctgcagc actcccacca aatgaaagcc agcaatacat ccacggatct tctccatctg
    48841 ataagtaaaa cgtcgagtct cctggacgaa tcaagaacag atcacctttt ttcaattgga
    48901 attgttgatt tttcacatga taagatcctt tgccttccat cacgacatgc aggatgtaat
    48961 cattgcgaat agcaggacca aaagaatgaa acggcatcgt tttagagaaa ccacaaaaat
    49021 caaaatacaa ttctttttct acttgttgat gttccttttt taaaaaaatc gattgctcca
    49081 cgtctatccc tccttactcc tttagtataa tgcaacaaaa agttaaataa aagtcacaga
    49141 aatatattaa aaaggaagcg atttcttgtt aagtttaagt cattaaaaga tctttgttat
    49201 tcgttgtaac cggtttactt agtattatat attgaacagg agaggaatac agattatgaa
    49261 aagcaaaatg atccaacgtg tgtcctacgc tttaggcgct tttggacacg acacattcta
    49321 tgttacatta agcacttatt ttatggtatt cgtgacaagc cagatgttcg caggaacaga
    49381 taaggcaacg aatgcaaaaa tgattgctac agttacaagc ttagttgtag gtatccgctt
    49441 agttgaaatt atttttgatc ctttgattgg tggcatcatc gataatactc gtacaaaata
    49501 cggtaaattc aaaccatggt tagttatcgg cggcgttgtt agttccgtac tcttggtagc
    49561 aatctttacc aatttctttg gcttagctac tcataatacg actttattta tcatattatt
    49621 tgcaatcgtt ttcatcttat tggatagttt ttattcattt aaggatatcg ctttttggtc
    49681 aatgattcca gctttgagta cggaaacaaa ggaacgtgaa aaatttgcta cgtttgctcg
    49741 tttcggctct tctctaggtg caaatggtac tactctagtc gttgtcccta tcgttacttt
    49801 ctttacctat cttgttactc ataaacatat tgaaggcgct tcaggctggt tctggttcgg
    49861 tgtgattgct gctgttattt caggcggtac tgcgattatc actgccttgg gcacaaaaga
    49921 agtcgatact gcgatccgtc agcaaaatga aaaagctggg atcaaagatg tgttcaaagc
    49981 agttgctcaa aatgatcagt tgatgtggct tgcccttgct tatatcgctt atgcgattgc
    50041 caatgcagca acaactgccg tattattcta cttcttcaaa tatgtacttg gccaaccaac
    50101 tgaattttgg atcgttggtc tagttgctgc cttacttggt ttagtagctg ttccactatt
    50161 cccaatgctt acacgcgtca tctctcgtcg ctacgtttac cttggaggga tcgcaatgat
    50221 gatttcttct tactttttct ttacgatcgc aggttcaagt ttgccaatcg ttatcatcgg
    50281 gctagtattg ttctacttcc ctcaacaatt gattttctta tccgctttga tgacgatcac
    50341 agactctgtt gaatatggtc aatggaaaaa tggtatccgt aacgaagctg ttactctatc
    50401 aattcgccct ttactagata aaatcgctgg tgctttgtct aacgggatcg tagggtttgt
    50461 cgctatcgct tctgggatga ctggtaatgc tactgctgct gatatcactg ctcatggcgt
    50521 aacgactttc aaagcttatg cattctatgc tcctgctgct ttgatgattt tctccgctat
    50581 tatttttgct tggaagatta cattgacaga gaaaaaacat gcacaaatcg ttgagaaatt
    50641 ggaaaacaaa ttggctccag ctgatacaat ggaagattaa cttcacgatg cagtagttcc
    50701 ttcccattaa gaaaggatct tgaaaatgaa gattacagaa aaagattttg gccaaggtta
    50761 tcacttgata accttggaga acaaaaacaa gctagcactt tctatctctg atttaggtgc
    50821 tagaatcgtc agtttaaaat caaatgatcg agaattagtt ttgggatttg acaccgctga
    50881 agaatatatc gaaaaagatc cgtatatcgg tgcttctatc ggtcgcaccg cagggagaat
    50941 cgaaaatggc cgcttttctc tcaatggaaa aacttaccaa ttagccacag atccaaaaac
    51001 tggacataat ctgcatggtg gtgctcctgg atttgaatta aaaaaatggt cttatgtcat
    51061 tttgaatggc gaaaatgaag cttctgtcat ttttactact acaagtcccg acggagaaca
    51121 tggttttcca ggtacaatgg atgtggaaat ccgctatact ttgacaaaag ataatatctg
    51181 gcgagtgacc tctcgcggca ccagtgacca agatacttta ttcaatccga caaatcatgt
    51241 ttacttcaac ttgacgggag atgcttctca gtcaatcgat caacatgagc tttggctcaa
    51301 tagcgaagct tatgctcctt tgcgaacaga tagtattcct ataggggtaa aagaaaatgc
    51361 tgcaggaagt gctttcgatt ttcaaatacc taaaaaatta gcgtccgttt ttgctagtga
    51421 tttggatcaa aaaaatcttg ttgacggaat cgatcatcca tttttcttaa aagaaaccgg
    51481 cttgggtaaa gaagccgctc gcttgactag ccccgataaa agaatccaag tagatattgc
    51541 tacagatgct tctagtgtgg ttattttcac tgcaaatttc ggcacagaaa caccagaaat
    51601 gcgcaaccga aaactggcac atcacggtgg catcacattt gagactcaaa cagctccagg
    51661 tgcagagcgc ttctcagcat ttggttcgat tcatttaaaa gccggatcag tatttgaaac
    51721 agtaacagaa ttcaaaatca aaacaagaaa ggaataactc atcatggaaa tcaaaagaag
    51781 cttaaacaca aaatttgccg aaacattcgg acctaaaaaa actacacaat acttctcacc
    51841 aggacgcatc aatttgattg gtgaacacac agattacaat ggaggccatg tcttccccgc
    51901 ttccatcact tatggaacgc aaggtgtagc agcacctcga gacgataata aaatattagt
    51961 ttattccaca aactttgacg atatcggtgt cattagtttt actttagatg agctagaata
    52021 tgataaaaaa gcaaactggg caaattacgt aaaaggaatg gtccttaaat taaaagaagc
    52081 tggacactca atcgatcatg ggtttgagtt attagtggaa ggaacaatcc ctaatggtgc
    52141 aggactttcc agtagcgctt cattagaatt attagtaggt gttgtattag aagatctgtt
    52201 tgatttaaaa acagatcgct tggaactagt ccaaacaggt aaaaaagtag aaaatgaatt
    52261 catcggtgtc aattcaggta tcatggatca gtttgccatc ggcttcggtg agctagatca
    52321 tgcaatctta ttagatacaa atacgctgaa atacgaaatg gttccagtga aactagatgg
    52381 ttatgcaatc gtcatcatga atacgaataa gcggcgggaa ttggcagatt caaaatacaa
    52441 tgaacgccgt agcgaatgcg aagaagcctt ggcacgtttg caaaaaactt tgcctatctc
    52501 ttctttagga gacttagatg aagaagaatt tgaatcaact attgaccaaa tcggcgatga
    52561 tacattgata cgcagagctc gccatgccgt ttctgaaaat cagcggactt tgaaggcaaa
    52621 agctgaatta gaagctggga acttagaagc atttggtcaa ctgttaaatg attctcacca
    52681 ctctcttcgc tatgattatg aagtaactgg gatcgaatta gatacactag tagatgctgc
    52741 tcaaaaacaa gaaggtgtct taggtgcacg aatgacagga gctggatttg gtggctgtgc
    52801 gattgctctt gtcaaagaag aaaatatacc tgccttcaaa aacaatgtct atgatgaata
    52861 catgaaagtt gtcggctatg ctccggaatt ttatgttgca cacattggaa atgggacaac
    52921 aaagatcgat ccagaagctg ttcatgtttg ataaaaaaat caacgatctc agctaaaaac
    52981 aaatagataa aaataacctc tcagcaattt ccaaatgtga gccttttgct cgtatttgaa
    53041 aattgctgtt ttttattgac acggaaagag aacgtgtgtt ctaattgtgt tgaagaggtg
    53101 attgttatgt gggcgaatca aaatccggta tttgtttatg aaaaagaacc ttcaagagat
    53161 attttatgta ttgactgcaa atctttttac gcctccgtag agtgcgttga aaaggatctt
    53221 gatcccctta caactaaatt agtagtcatg tcctatccgt ccgattcgac agaaacacgg
    53281 ggtagcggat taatcttggc ttccagtcct gcagcaaaaa aagcttacgg catcaccaat
    53341 atcagccgtg ccagagatct tcccttcccc tatccaagtg atttatatat tgttgcccca
    53401 aggatggcct attatatgga aaagaacaga gaaatcaatt ctatctataa aaaatatgca
    53461 gacgaagcga accatcatgt ctattccgtc gatgaaagct tccttgatgt aacaaatagc
    53521 atggcactct ttggtaaaac aaatgcgtac gacatggcta aactgatcca taaagatgtg
    53581 tttgagcaga ccggaatcta cacgactata ggaattggcg ataacccgct acttgctaaa
    53641 ctagccttag acaatggctc aaaagacgga aagcaaatgt ttgctgaatg gcgttatgaa
    53701 gatgtaccag aaaagttatg gaaaatctca gatatcactg acttttgggg aatcggcaaa
    53761 cgaacagcaa aacgtctgaa acaaatgggt atacattctg tttatgattt agctcacgcg
    53821 gattattatc acttgaaaag caagctaggg gtcatcggta ctcagcttta tgcccatgct
    53881 tggggcatcg atcgaagttt tttgggacaa gaatatacac caaaatcaaa aagtatcgga
    53941 aatagtcaaa tcctagatag agattataca agacgagaag aaatcgagat cgttatcaaa
    54001 gaaatggccg accaagtcgg cactcgcttg agacgatctg cagcaaaaac acaagtagtc
    54061 agcttatggg taggatactc tttaggctat cttgatccaa atggcaagac cgggtttcac
    54121 cagcaaatga aaatcgaagc gacaaatgct agcagagatc ttgcagatgc tttattgacc
    54181 attttccacc agcattacca ctaccaggat attcggcgtg taggcgtgaa ctgttccgac
    54241 ctcgtatacg cgacaggttt acagcttaac ctttttgaag atcctgaaaa gcagataaat
    54301 gaagcgaaag ttgattttgt tgtcgatacg attagaaaaa aatacggctt tcaatcaatc
    54361 atccatgcgc atagtctctt gcaaggagga cgcgccatcg cacgtagttc tctcgttggc
    54421 gggcatgcag gtggtatgag tggattagaa ggtggaaaag atgagacgga cagcaaaaaa
    54481 attcattgac tataacgagt atcgagatcg gccatttgga ttaaaatggg ggactgcttt
    54541 tgcgatggaa gaactagtca acggtatcaa aaaaatgaag aagaagcacg caaacagcct
    54601 cttcctaaac aacaaatgac attgcaagaa atcgatagta tactgacaga agccttttta
    54661 tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg aatttggaag actcttggat
    54721 gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt actttatgat tgaccatcag
    54781 ccgatattgt gggaagacgt acggtatatt gagttaaagg aaacaaaaaa atggtttgtg
    54841 acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat aagaaaggat ttttcgaaaa
    54901 ttgcttctta aatttttgtg gaattttggc ttatttttcg aagaagcaac ttctggaaca
    54961 acgtatatta gatatcagag gttgtgaaaa agtcgcttag ttttgagtaa caagccaagg
    55021 cgctgatttc acaacctctg tctatctatc catctattcg gtaagattca tgcatgacgt
    55081 cttctttatt tagtatgtag agcccttttt cttctgacca ttcaacataa atcagctgtt
    55141 ttacatctgc atagccttct ttcttcaagt tctccataag ccatgcttca gtcaattgat
    55201 tggactgaag cactttatgc tgaatttgcc cttcatctac aagcaggatc gataaagctt
    55261 tgtcttcatc tgattttttc aatacactca tttgaccggc attctctagg ataacatgtt
    55321 tggcattttc aagaaaaaag cattcttgct gtcttaagat tgctcgcaac tgctccatct
    55381 cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc gtctttgatt agaacagatg
    55441 gctcgccttt gacccaacga ttgacctttt catttttttg gacgatacgt tcgataccat
    55501 aaatcattac tgcccaaagc gcaatggcaa ataggacatg ccctacatgg acattgtcat
    55561 cataggttga ttcttctaag atcccaccta aaacaagcgt atagactaaa tcaaacggtg
    55621 tgatttctga catggatttc ttcccaagca aacgtaccac taataataaa ccgatcaacc
    55681 cgaacaccag tttcattgta attgaaaaaa tcattttttc tccccctaac tatctgttaa
    55741 ttaaattata tatgctttct cacgaacacg catggaatat acaaggaaga aatgtatcct
    55801 tttactaaaa aaaggactgt gacactgtag tgacccccaa aagttagact tttttggagt
    55861 ggagaaatcc actccttttt tttatgctct aaaatgatat ttttacacaa ccgttacggt
    55921 tgttttacga aattggacg
//
LOCUS       DAYYHS010000011        51482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000011 DAYYHS010000000
VERSION     DAYYHS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.011"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..194
                     /locus_tag="ACHA6A_000649"
     CDS             <1..194
                     /locus_tag="ACHA6A_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968878.1"
                     /translation="SFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSV
                     TDRHWQLVEDADLSYLPLK"
     gene            complement(275..844)
                     /gene="thiT"
                     /locus_tag="ACHA6A_000650"
     CDS             complement(275..844)
                     /gene="thiT"
                     /locus_tag="ACHA6A_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8968879.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(910..1012)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1018..1989)
                     /locus_tag="ACHA6A_000651"
     CDS             complement(1018..1989)
                     /locus_tag="ACHA6A_000651"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8968880.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2083..3456)
                     /gene="glmU"
                     /locus_tag="ACHA6A_000652"
     CDS             complement(2083..3456)
                     /gene="glmU"
                     /locus_tag="ACHA6A_000652"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8968881.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(3959..4777)
                     /gene="purR"
                     /locus_tag="ACHA6A_000653"
     CDS             complement(3959..4777)
                     /gene="purR"
                     /locus_tag="ACHA6A_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8968882.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(4944..5768)
                     /locus_tag="ACHA6A_000654"
     CDS             complement(4944..5768)
                     /locus_tag="ACHA6A_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8968883.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5741..6421)
                     /locus_tag="ACHA6A_000655"
     CDS             complement(5741..6421)
                     /locus_tag="ACHA6A_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8968884.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6444..7382)
                     /locus_tag="ACHA6A_000656"
     CDS             complement(6444..7382)
                     /locus_tag="ACHA6A_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8968885.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7627..8478)
                     /gene="ispE"
                     /locus_tag="ACHA6A_000657"
     CDS             complement(7627..8478)
                     /gene="ispE"
                     /locus_tag="ACHA6A_000657"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8968886.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8632..11205)
                     /gene="mprF"
                     /locus_tag="ACHA6A_000658"
     CDS             complement(8632..11205)
                     /gene="mprF"
                     /locus_tag="ACHA6A_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8968887.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11346..12152
                     /gene="proC"
                     /locus_tag="ACHA6A_000659"
     CDS             11346..12152
                     /gene="proC"
                     /locus_tag="ACHA6A_000659"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8968888.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12231..13787)
                     /gene="rny"
                     /locus_tag="ACHA6A_000660"
     CDS             complement(12231..13787)
                     /gene="rny"
                     /locus_tag="ACHA6A_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8968889.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14198..15247)
                     /gene="recA"
                     /locus_tag="ACHA6A_000661"
     CDS             complement(14198..15247)
                     /gene="recA"
                     /locus_tag="ACHA6A_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8968890.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15356..16597)
                     /locus_tag="ACHA6A_000662"
     CDS             complement(15356..16597)
                     /locus_tag="ACHA6A_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8968891.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(16837..17415)
                     /gene="pgsA"
                     /locus_tag="ACHA6A_000663"
     CDS             complement(16837..17415)
                     /gene="pgsA"
                     /locus_tag="ACHA6A_000663"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8968892.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(17624..18547)
                     /locus_tag="ACHA6A_000664"
     CDS             complement(17624..18547)
                     /locus_tag="ACHA6A_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8968893.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(19286..20572)
                     /locus_tag="ACHA6A_000665"
     CDS             complement(19286..20572)
                     /locus_tag="ACHA6A_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8968894.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(20565..21833)
                     /locus_tag="ACHA6A_000666"
     CDS             complement(20565..21833)
                     /locus_tag="ACHA6A_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8968895.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22085..22546
                     /gene="mscL"
                     /locus_tag="ACHA6A_000667"
     CDS             22085..22546
                     /gene="mscL"
                     /locus_tag="ACHA6A_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8968896.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(22605..23051)
                     /locus_tag="ACHA6A_000668"
     CDS             complement(22605..23051)
                     /locus_tag="ACHA6A_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8968897.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23144..23389)
                     /locus_tag="ACHA6A_000669"
     CDS             complement(23144..23389)
                     /locus_tag="ACHA6A_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8968898.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(23504..24082)
                     /locus_tag="ACHA6A_000670"
     CDS             complement(23504..24082)
                     /locus_tag="ACHA6A_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8968899.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24159..24689)
                     /locus_tag="ACHA6A_000671"
     CDS             complement(24159..24689)
                     /locus_tag="ACHA6A_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8968900.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(24693..25553)
                     /gene="rlmB"
                     /locus_tag="ACHA6A_000672"
     CDS             complement(24693..25553)
                     /gene="rlmB"
                     /locus_tag="ACHA6A_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8968901.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(25546..25941)
                     /locus_tag="ACHA6A_000673"
     CDS             complement(25546..25941)
                     /locus_tag="ACHA6A_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8968902.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(25938..27350)
                     /gene="cysS"
                     /locus_tag="ACHA6A_000674"
     CDS             complement(25938..27350)
                     /gene="cysS"
                     /locus_tag="ACHA6A_000674"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8968903.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27347..27883)
                     /gene="epsC"
                     /locus_tag="ACHA6A_000675"
     CDS             complement(27347..27883)
                     /gene="epsC"
                     /locus_tag="ACHA6A_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8968904.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(27969..28208)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28205..29665)
                     /gene="gltX"
                     /locus_tag="ACHA6A_000676"
     CDS             complement(28205..29665)
                     /gene="gltX"
                     /locus_tag="ACHA6A_000676"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8968905.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(29835..30992)
                     /locus_tag="ACHA6A_000677"
     CDS             complement(29835..30992)
                     /locus_tag="ACHA6A_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8968906.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(31026..32396)
                     /gene="radA"
                     /locus_tag="ACHA6A_000678"
     CDS             complement(31026..32396)
                     /gene="radA"
                     /locus_tag="ACHA6A_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8968907.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(32459..32986)
                     /locus_tag="ACHA6A_000679"
     CDS             complement(32459..32986)
                     /locus_tag="ACHA6A_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8968908.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33216..34022
                     /gene="proB"
                     /locus_tag="ACHA6A_000680"
     CDS             33216..34022
                     /gene="proB"
                     /locus_tag="ACHA6A_000680"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8968909.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            34019..35266
                     /locus_tag="ACHA6A_000681"
     CDS             34019..35266
                     /locus_tag="ACHA6A_000681"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8968910.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            35342..35686
                     /locus_tag="ACHA6A_000682"
     CDS             35342..35686
                     /locus_tag="ACHA6A_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968911.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(35803..36129)
                     /locus_tag="ACHA6A_000683"
     CDS             complement(35803..36129)
                     /locus_tag="ACHA6A_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968912.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(36744..37946)
                     /locus_tag="ACHA6A_000684"
     CDS             complement(36744..37946)
                     /locus_tag="ACHA6A_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8968913.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            38063..38578
                     /locus_tag="ACHA6A_000685"
     CDS             38063..38578
                     /locus_tag="ACHA6A_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8968914.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(38777..39223)
                     /locus_tag="ACHA6A_000686"
     CDS             complement(38777..39223)
                     /locus_tag="ACHA6A_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8968915.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(39270..40748)
                     /gene="msr(C)"
                     /locus_tag="ACHA6A_000687"
     CDS             complement(39270..40748)
                     /gene="msr(C)"
                     /locus_tag="ACHA6A_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8968916.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(41102..41731)
                     /locus_tag="ACHA6A_000688"
     CDS             complement(41102..41731)
                     /locus_tag="ACHA6A_000688"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8968917.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(41715..42179)
                     /locus_tag="ACHA6A_000689"
     CDS             complement(41715..42179)
                     /locus_tag="ACHA6A_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8968918.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(42666..43934)
                     /locus_tag="ACHA6A_000690"
     CDS             complement(42666..43934)
                     /locus_tag="ACHA6A_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8968919.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(44165..44902)
                     /locus_tag="ACHA6A_000691"
     CDS             complement(44165..44902)
                     /locus_tag="ACHA6A_000691"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8968920.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(44969..45538)
                     /locus_tag="ACHA6A_000692"
     CDS             complement(44969..45538)
                     /locus_tag="ACHA6A_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8968921.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            45666..46538
                     /locus_tag="ACHA6A_000693"
     CDS             45666..46538
                     /locus_tag="ACHA6A_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8968922.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            46880..47752
                     /locus_tag="ACHA6A_000694"
     CDS             46880..47752
                     /locus_tag="ACHA6A_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8968923.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            47749..49707
                     /gene="nagE"
                     /locus_tag="ACHA6A_000695"
     CDS             47749..49707
                     /gene="nagE"
                     /locus_tag="ACHA6A_000695"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8968924.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(50039..50890)
                     /locus_tag="ACHA6A_000696"
     CDS             complement(50039..50890)
                     /locus_tag="ACHA6A_000696"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8968925.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            51013..51480
                     /locus_tag="ACHA6A_000697"
     CDS             51013..51480
                     /locus_tag="ACHA6A_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8968926.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
BASE COUNT        14691 a        11038 c         9222 g        16531 t
ORIGIN      
        1 tttcttttga tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg
       61 aagattatat ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt
      121 taatctactc tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc
      181 tgccattgaa gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca
      241 tgattctgtc tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca
      301 caaaaatcga aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg
      361 tactcaaccc gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata
      421 gtgcatacgc tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg
      481 cgccaatcaa tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat
      541 agcctttttg gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg
      601 ggtactcaat caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta
      661 ggatgcccca aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa
      721 gagtcaatgg aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca
      781 acgacaacac catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc
      841 ccataaaaaa acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa
      901 atagtcaaac agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag
      961 ggtttagaaa tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta
     1021 tttatttttt gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc
     1081 atctcccatt agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg
     1141 aagatagatc gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa
     1201 agcagggccg gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc
     1261 tttcaaagca ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag
     1321 tacacaagtt ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt
     1381 tggacggcgt ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc
     1441 ccgtgtgaca ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt
     1501 ttctaagaaa taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa
     1561 gaaaccttgg atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt
     1621 ttgaatcatg tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc
     1681 ttgacgcgca tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt
     1741 cagtgcgtca atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga
     1801 ttgaataaca tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc
     1861 atcactaaat tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt
     1921 ctctgctaat gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg
     1981 ttttgacatg gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag
     2041 tagtcatatt ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag
     2101 tttttttgca tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc
     2161 tggaatattg tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac
     2221 aggtccgatg atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg
     2281 tttgttcttt ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc
     2341 cagcgtagca tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc
     2401 atttttcact tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg
     2461 taaatgtgca taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga
     2521 gttttcaatg acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat
     2581 gacacaatca gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt
     2641 gtctggaccg atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac
     2701 gccattgacc atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa
     2761 cgcgatccga tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac
     2821 tgtatggcct tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc
     2881 attgtttgtt ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt
     2941 tcctgtattg atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat
     3001 tttttctacg atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc
     3061 ttgtgccgtc aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt
     3121 cagcgtttca gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt
     3181 tccttccaag aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg
     3241 taacgcatat ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac
     3301 gatcgtaatc acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat
     3361 aggtttacca gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt
     3421 ccctgccgcc aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt
     3481 gctgatttca gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta
     3541 tcccagcgtt ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac
     3601 cggtgctctc ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct
     3661 gttttttgaa atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt
     3721 aaacgttttt caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag
     3781 caatttttcc gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga
     3841 taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa
     3901 agccattttc acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt
     3961 attctgaaaa ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt
     4021 tgacataaag cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg
     4081 caaatacagt gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt
     4141 tgactgttcc gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca
     4201 aacttctctt agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga
     4261 cacttactgt tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat
     4321 tcagataata ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag
     4381 catcgatttt ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt
     4441 caggttctcc taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt
     4501 ctgatagacg ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag
     4561 ggatgaacaa tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt
     4621 ctttaaatgt tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat
     4681 aacgttcggc aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg
     4741 tcatgtcaac tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa
     4801 ttttatcgtt ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat
     4861 aagaaaatag ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt
     4921 ataaggcttc ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt
     4981 ttgatgatat ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga
     5041 ggtgtatcta gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa
     5101 acactgatag caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga
     5161 agaaccatga ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc
     5221 acaccagtaa tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg
     5281 gcggtatctt catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata
     5341 ccgatggcaa cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc
     5401 gaaccaaaca gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat
     5461 actaacgcta gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt
     5521 cggtagatcg ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc
     5581 gtaagtgtag gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga
     5641 gaaagcgtat cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg
     5701 gcgattccgg caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc
     5761 aacgccattg agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga
     5821 tatcttcatg atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc
     5881 ggagaagacg ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa
     5941 gaacaaataa gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc
     6001 cgccagacaa ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag
     6061 cacgctgtac atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat
     6121 atcttcctga acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa
     6181 cttgttgagg aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct
     6241 ttatcattcc ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg
     6301 ctgctccatt ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat
     6361 cttctagtac aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca
     6421 ccttacaacc tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt
     6481 tccatgacag aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa
     6541 ggattcaaca cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa
     6601 gaggcatttt cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc
     6661 tcagctaagc gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt
     6721 tttaagtcgt attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg
     6781 tttgttgcat cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata
     6841 taagcatcgg cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct
     6901 tcagcaatcg ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg
     6961 tattcatgtg aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat
     7021 tctaaacggt cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa
     7081 tcctttaacg aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca
     7141 tcggaaattt ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca
     7201 gggatcaaaa gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc
     7261 ggataaaaag tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt
     7321 ccacatgctg caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc
     7381 atttatatgc tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat
     7441 gtatcaaaat aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat
     7501 attcttgatt aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa
     7561 taaaaaactg aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct
     7621 tatattttat tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt
     7681 atatactctc tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc
     7741 actcatcaat gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac
     7801 tggatgccgc tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg
     7861 ataatcttgc tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg
     7921 cgtatctact tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac
     7981 aacccagcac tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa
     8041 tgccgtactt ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc
     8101 cagttcttct aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc
     8161 acagtctgta cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat
     8221 cttgacaccg gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt
     8281 gttccgttca tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt
     8341 tatttcttca aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc
     8401 atgataccca tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc
     8461 tttttcaatc aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc
     8521 atagggaaaa ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca
     8581 acagctttca cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc
     8641 tgtgagcact tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag
     8701 tagctgcatg gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc
     8761 ccaatgatcg acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt
     8821 accataatta tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc
     8881 aacattagct aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac
     8941 aataaacaga ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg
     9001 caacaaatca tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac
     9061 tttttgatct ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg
     9121 tcccacagag aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac
     9181 agcttgatgt aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat
     9241 caaacgttga tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac
     9301 aatccctgct tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta
     9361 gctttctgat atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa
     9421 ctgattcgtt gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca
     9481 tttgtttcct ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata
     9541 aaaataactg taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc
     9601 caataaacgg tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc
     9661 taaatattga taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga
     9721 gaaccacaca tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa
     9781 aaagcgtccg ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct
     9841 agcatcaaag attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct
     9901 ggataaccac acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt
     9961 acgtgtgatt gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt
    10021 tttgactttt actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt
    10081 cactgtctga tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat
    10141 tgcagacaga ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc
    10201 acttacgagc acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa
    10261 tcgattgatt ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag
    10321 aacataataa aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga
    10381 caattgactc attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat
    10441 cgtcagcatt cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat
    10501 atctattctc atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc
    10561 aaaggatgcc agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt
    10621 tctctttaga taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca
    10681 gatccaatag cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag
    10741 aaacgaccat acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa
    10801 aaccattttt ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac
    10861 accaccaaaa ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc
    10921 ttttctttcc atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata
    10981 caacaacatc ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg
    11041 gctttgttgt cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc
    11101 gacttgattt gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact
    11161 atgttccttt atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta
    11221 acttgtatac ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt
    11281 agacctgttc tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg
    11341 ataacatgaa agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt
    11401 tattaaaaaa aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa
    11461 atgcacaaaa atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg
    11521 catcacaatc agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg
    11581 aagaactaag agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat
    11641 taatagattt agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga
    11701 atgtcaatgt cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg
    11761 aggaacagct tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag
    11821 cagaatcaga tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca
    11881 tgctgatcga tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca
    11941 ctgagattgc tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa
    12001 atccatggga acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag
    12061 ttgccttaga agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg
    12121 agaaagatct tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca
    12181 aaaaaagaag agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca
    12241 taatcagttg cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt
    12301 tcttcttcga ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt
    12361 tcttctggct tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct
    12421 acaccggcga agctgttcga aatattttcc agacttctta aacgacggat gtagttttct
    12481 aatgattcac ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg
    12541 acagagataa cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact
    12601 actgggtttt ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca
    12661 atctcatggt caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg
    12721 atatcttcgc caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc
    12781 ttcaagacgt tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga
    12841 tctggatgaa gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata
    12901 cgttcatcca tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt
    12961 cgtccatctt gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg
    13021 aatccggaaa gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc
    13081 gtctcaagcg ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca
    13141 ttcggaaggg taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga
    13201 atcgctaatg ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca
    13261 gattctttta ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg
    13321 acatctcgtg cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct
    13381 tcaatcattt tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt
    13441 gcttcttctt tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc
    13501 agcgtttgtt cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct
    13561 ctcagctcac tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat
    13621 gcttcttttt ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat
    13681 gattgtgcac cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca
    13741 agaccgacaa ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc
    13801 atactatctt tttcattttt atcatctata cagcttctat gataaattat tctaaactcg
    13861 tcaatatgca tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt
    13921 gtaccccgta gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac
    13981 gatggttttt aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact
    14041 tttctattaa attattcttt gtagatgaac agatattttt attttatgta cccactcgta
    14101 taattaaata actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc
    14161 tactcttcag accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt
    14221 cttcttgtcc tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc
    14281 taactagagc agaaacttct gccatcattt caggatgatt agccatatag attttagcgt
    14341 tttcccgtcc ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa
    14401 caatgtcttt ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat
    14461 acatgacatc tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga
    14521 tctttgtacg gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt
    14581 ccaaacggat cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc
    14641 caaacatcac gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt
    14701 tgttgatcga accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga
    14761 catgtgagtc acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat
    14821 cgactactac gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt
    14881 gttctcctgt gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg
    14941 catattgcgg atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt
    15001 gtacttctgc aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata
    15061 cttcgatgat acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag
    15121 agccacttgg aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc
    15181 ctttaccata gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat
    15241 ctgccaatac gataatcctc cttatattta caaatcagag caacatttca ttatccttat
    15301 catatctttt ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt
    15361 ttcttaaaag ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag
    15421 cagcacgatc tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag
    15481 cgatccatac tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga
    15541 aagctacact gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac
    15601 attctttgct gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag
    15661 cttttgtttg gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa
    15721 tgtcgcctaa tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct
    15781 gcttgttttc tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc
    15841 cataaaaata ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat
    15901 tccctgcctg aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg
    15961 catatggtgc gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca
    16021 gtcgcgattc accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag
    16081 gaaaacgttc ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag
    16141 gcggaccggg aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa
    16201 gccctgtacg atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt
    16261 ttttggtcat ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg
    16321 tattttgtat gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt
    16381 ctgtcggtcc taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa
    16441 tcaactcttc taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac
    16501 ctagatcagc tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta
    16561 aaagttctgt tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt
    16621 atacgctgta tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc
    16681 ttgtcacagc tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt
    16741 cacaatatac ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt
    16801 agacggcagg ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc
    16861 gctgtttttg atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag
    16921 catgatttga tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa
    16981 gataatagca accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac
    17041 ttctccatgt tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat
    17101 gatcgctact acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat
    17161 aactagcatt ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg
    17221 cgcacgggca atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc
    17281 acctactagc tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa
    17341 aggtaaacct acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt
    17401 atttggtaaa ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag
    17461 tttccaccga tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa
    17521 attgatcttt ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt
    17581 tttataccta caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa
    17641 gagtaaggtt cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt
    17701 ctccattcgc tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg
    17761 ctgtttccgg taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat
    17821 aggcattgtc tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta
    17881 caggcttggt agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag
    17941 ctattgccat tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt
    18001 tggtctgcgt gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg
    18061 aagaagctgt gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca
    18121 cgacaacgac aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa
    18181 agcgacttgg atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag
    18241 gctgcggacg ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta
    18301 aatggtctcc atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa
    18361 atttaccagg caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa
    18421 tttttgtaat ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta
    18481 atttttctcc gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc
    18541 gaagcatctg ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc
    18601 acagctcctt ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca
    18661 atatccaata aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac
    18721 ggcaggctca cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc
    18781 aaaaaccggc aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa
    18841 acgctgattt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    18901 gtccaaccaa gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat
    18961 gcagggcagc tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca
    19021 ataagccgaa atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
    19081 gaagctgacc acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata
    19141 agttgattaa aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta
    19201 agaataccat aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta
    19261 agacttttcc tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct
    19321 cttcacttat aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga
    19381 tttcaggaag atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa
    19441 tatattcgat cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat
    19501 cgagatttgt ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt
    19561 tttctgctgc tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac
    19621 gatccaagct gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta
    19681 gatagtttcg agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat
    19741 agcgtaacag ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa
    19801 atttatctcg tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt
    19861 cagggaaata acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc
    19921 gaataagttt catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc
    19981 ttggctgata aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac
    20041 tttcaaccgt gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta
    20101 tgccaaaaaa catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga
    20161 ttcctttttc cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca
    20221 gagtcgtcag attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa
    20281 aacttgtgaa agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat
    20341 cttctttttc aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct
    20401 tttctttttc accataggga ataaattcgt tatcgatcga accataattc gtactgaaca
    20461 aaccatatgt cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca
    20521 gtacttcatg ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt
    20581 cctcctgcta aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct
    20641 ttgatcgtta cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc
    20701 agccagctgt ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga
    20761 tattggtttc tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt
    20821 cgcaaacttt ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg
    20881 ataccggttt ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa
    20941 gctagacttt ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat
    21001 aaaccgttaa agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga
    21061 taagctaggt tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata
    21121 ttcacttgag gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt
    21181 aattgcccaa ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg
    21241 atttgatcat ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg
    21301 tccaaatctt cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa
    21361 agttcttgca aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat
    21421 agacgcagat tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata
    21481 tttggataaa ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca
    21541 ttgacatatt caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt
    21601 tttctaactg ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt
    21661 gtctgatccg aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc
    21721 gtgatgactt cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat
    21781 tgttttgttg gtaagacatg aagattcacg ccttctgcca attgatacac catatttata
    21841 tgctcctttt cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta
    21901 cttattcctc tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt
    21961 tacactttat agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta
    22021 tattgttttt ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc
    22081 attcatgatc aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt
    22141 cggcgttgtt attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat
    22201 cacaccacta ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc
    22261 actttcaatt cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat
    22321 tagtgctatc attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc
    22381 aaaccgtgcg aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac
    22441 tgcagaagat tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa
    22501 tacacatcca gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa
    22561 aaaagaaagc cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt
    22621 tcctttttca tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg
    22681 aatccaaacg actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt
    22741 tctggaaaat ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca
    22801 tctttacggt tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca
    22861 tatactcgcg tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct
    22921 tctccatcaa tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg
    22981 attcgttctt tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc
    23041 tgatattcca ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac
    23101 aaaaaagcaa acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta
    23161 aaaattcgat ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa
    23221 aagaattttc ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa
    23281 cgccgcggcg ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc
    23341 cgatacgacc ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct
    23401 ctactatcaa ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa
    23461 atgcaataaa aataaataag aagctcatta agatttttct aaattattcc tcattataaa
    23521 atttcaagtg tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt
    23581 gatccattgc taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa
    23641 aaattttagc ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata
    23701 aaagatgctg aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt
    23761 ctgtctctat tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt
    23821 tctgcttcct caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa
    23881 agctaacacc tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt
    23941 cttgttccca atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta
    24001 aaagtggaaa atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag
    24061 cgcgcaataa ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct
    24121 tatcatcaat gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa
    24181 tcgtccaatt tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg
    24241 aaatcaataa aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc
    24301 gtcttatata attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt
    24361 tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg
    24421 attctctcga tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg
    24481 tatttcttat aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact
    24541 tccattcctt catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc
    24601 gccattttgt cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag
    24661 ccgtctacaa tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc
    24721 tgatacataa gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc
    24781 gggatcgtca gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca
    24841 ttaccaatga ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc
    24901 atatctgtgc caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa
    24961 ttcgtcacgc gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata
    25021 ggtgtgattc caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct
    25081 gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag
    25141 aaggggtctt cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca
    25201 taaggtgtaa tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat
    25261 ttttgtttag gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct
    25321 tctacttttt ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt
    25381 agcgcttcta ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt
    25441 tgatcagaaa ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt
    25501 ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc
    25561 accaactttc tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa
    25621 atgcagatag cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc
    25681 attttttgcg ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc
    25741 ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga
    25801 aacataattc gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag
    25861 gtggtcacga acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt
    25921 caattgtgtg taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct
    25981 aataagatcc ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga
    26041 ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct
    26101 tctgctgtaa ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt
    26161 gccaagacag cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc
    26221 tcatagacca ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat
    26281 cgctgttcta aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag
    26341 gcagtttctt ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg
    26401 gctgcttctt tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc
    26461 atgaagaaac gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa
    26521 ttccctagag atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc
    26581 atccagtaat tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca
    26641 tgatgaggaa attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt
    26701 tttgttgcca taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa
    26761 tcccaagaaa tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct
    26821 ttttgctgct gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat
    26881 tggtcactta atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa
    26941 gattcataag catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata
    27001 tgatccatga ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct
    27061 tcaaatgctc ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct
    27121 gtacggatga ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct
    27181 cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata
    27241 tggatataat tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata
    27301 gggatgaatt cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc
    27361 tcactttttc tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt
    27421 ctggaacatc tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta
    27481 ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc
    27541 ttttacccgt ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt
    27601 cctcgatttc tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag
    27661 ttccaatcgt tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat
    27721 tgatcttcgc caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa
    27781 acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat
    27841 cgtttctctt tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc
    27901 gtaagaattt tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag
    27961 tttccttatc aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc
    28021 tctgacagct aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt
    28081 cctgttttct gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat
    28141 accttgcacc acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc
    28201 gtgtttagtt cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga
    28261 tcgtttcagg caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga
    28321 aaagattttt tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag
    28381 ttggagcatc tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag
    28441 gaactgtttc accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga
    28501 aaaatagcgt agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc
    28561 taacgatctt cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat
    28621 cagcgattag ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt
    28681 aatggttgtt tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc
    28741 gttttggatc gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag
    28801 gtgaccagcc caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc
    28861 gatattgttc gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt
    28921 cactgttgat gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat
    28981 agatcatcag ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga
    29041 ttttcatcaa atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt
    29101 tttggatgac aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca
    29161 tatctgtaaa tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac
    29221 cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat
    29281 gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc
    29341 aatagcattt gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt
    29401 ctttacgttc agattgacgg taagggccgt attctccagg attttcagga gattcatccc
    29461 aattcattcc caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac
    29521 gtttttggtc agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca
    29581 aataattgaa taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg
    29641 catagcgtac gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa
    29701 cagcaaaaat tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac
    29761 aaattttacc ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg
    29821 gctgaatttc ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg
    29881 attcctcgtc ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg
    29941 gcacttgtta caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca
    30001 accatcgtgc cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact
    30061 actagaacat tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca
    30121 ttgatattaa gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg
    30181 gttacaatga caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg
    30241 atatcttcaa agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca
    30301 tttaggatat ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg
    30361 atgtattgta gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca
    30421 gtctttgtaa tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat
    30481 ttatggaaat tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt
    30541 tcttgctcga tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc
    30601 gttcccattc tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta
    30661 ttgacgaatg gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat
    30721 ccgatgatcg caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt
    30781 tgttctactt gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag
    30841 aaaataagtg cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt
    30901 tgaccgattg cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg
    30961 atcatcagca aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt
    31021 attagttagc ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg
    31081 attccttttg gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag
    31141 ccgagttttt ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca
    31201 gtcagtccaa tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt
    31261 gaagcaatac tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct
    31321 gcagctttta agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact
    31381 gccatgatca gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg
    31441 aacatagtag gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt
    31501 gccacaacga tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa
    31561 ggattagcga cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt
    31621 gagccaaatc ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca
    31681 aaatataaca ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct
    31741 ttggtaacat gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt
    31801 tctgcagtgg tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc
    31861 gtcatcgttt ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga
    31921 ctgatttcgt tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg
    31981 cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt
    32041 ccgcctatgg ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct
    32101 ggatcgccac caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc
    32161 agttcttcta attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt
    32221 gttggttttg ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt
    32281 tcgatttctt cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta
    32341 ggcgacacat acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta
    32401 ccgcctcctt ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt
    32461 atttactgga agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt
    32521 ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct
    32581 tgaaaccaaa gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat
    32641 aatcactgtc aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc
    32701 ttgaacggtt agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag
    32761 gcacgagggt tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg
    32821 cgactgtttc caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt
    32881 ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat
    32941 tttgatcttt atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct
    33001 aaataaacta ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct
    33061 tcaagaaatt ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca
    33121 agttgtacaa aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac
    33181 gaaattaatg agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa
    33241 aacgaattat tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc
    33301 ttgcggcaat cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag
    33361 tgatcttagt ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac
    33421 gtccgacttt gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga
    33481 atatctataa tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa
    33541 gagacgtggt agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt
    33601 tgattatgga cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag
    33661 atcatctgac aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg
    33721 cggcagacct tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta
    33781 caaatcccga ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac
    33841 aagcaggtgg tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg
    33901 cttctaggat atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa
    33961 ttatttttga tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat
    34021 gactgacttg atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat
    34081 gcctacaaac cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca
    34141 acaagcgatc attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag
    34201 tgaaacgatg caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga
    34261 gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa
    34321 caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta
    34381 cgaatctcgt ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc
    34441 cgttatcctt cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt
    34501 gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac
    34561 ttctcatgaa actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc
    34621 tcgaggcgga gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga
    34681 aacaggtaca ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt
    34741 gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt
    34801 gctgatccat tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga
    34861 acacggggtc tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt
    34921 agcagaagac acagattggg atactgaata tctagattat atccttgctg tcaaagtggt
    34981 cgattctttg gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac
    35041 gatcgtgaca gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc
    35101 tgtctatgtc aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga
    35161 gattggtatc tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac
    35221 ttccacaaaa tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa
    35281 cattattccc gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa
    35341 tatgtttgaa attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt
    35401 tctgggagtc ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca
    35461 acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt
    35521 gcacattagt tacatggttg cattatgctt attagcgaca atcaattact attctttttt
    35581 cgatcattca atcgcttata cgattacgct actgattgct gggatttttc acttatctta
    35641 taaatatcag ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg
    35701 ataagtagag tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa
    35761 agattttgtc acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa
    35821 atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg
    35881 aagcagctca tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc
    35941 cagtagctgt tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct
    36001 agtttcacct tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg
    36061 atcatctgaa aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc
    36121 ttccagcatc tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa
    36181 aattttccag tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc
    36241 ccctttttcc gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc
    36301 aacaaacatg agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt
    36361 ctcacaacct cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac
    36421 ggtaaaatat gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg
    36481 attttacaac ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa
    36541 cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg
    36601 attttacaac ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac
    36661 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca
    36721 gctttttcac aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga
    36781 taaatgatac aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt
    36841 tcaccaatat gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag
    36901 cggacaaaac tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca
    36961 atcgtcgtca acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc
    37021 ggtacaagga ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag
    37081 acaaatgctg ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg
    37141 aaaatcgaag caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat
    37201 tcagaatagc caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta
    37261 tataacaatg cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct
    37321 tcccaaaggc ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct
    37381 ataaaaccgc aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg
    37441 cccaagaccc caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac
    37501 atcatgattg ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta
    37561 gagacaaata aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata
    37621 ttcccagata atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat
    37681 gtttttttca aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg
    37741 actaacatgt aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca
    37801 gcaatcgttt ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta
    37861 gctgctacaa ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata
    37921 tctatttgat tctccaatat ttccatccct ttctctctat tagcatcata catgatttat
    37981 tttacatgtc aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa
    38041 taagacgaga ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt
    38101 atccaaacca cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca
    38161 tcacagtcag atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt
    38221 ggacaaagac gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct
    38281 tgtagaggaa tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc
    38341 aaaaatctat gctttttcga cgctcgataa aacaacagct actggactgc tattcactag
    38401 agaacaacag ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct
    38461 agacaagaaa caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca
    38521 gcaggaactc caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg
    38581 gataaacaga acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc
    38641 aatacagttg gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca
    38701 ctatttttct agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc
    38761 aaataacagt gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg
    38821 ggccaacgat gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca
    38881 tataagcaga gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca
    38941 tacatgtcac cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc
    39001 tttctggatt tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga
    39061 catccaaaat gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat
    39121 gaagatcttg atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca
    39181 tcattgttgt aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta
    39241 aatttgtcac gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt
    39301 ttctgccctt ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga
    39361 gaagtaaaca aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt
    39421 gccatatcaa taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa
    39481 agcaaagcaa tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc
    39541 aaagtttcct gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac
    39601 tctgtttgtc tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt
    39661 tgctgatata cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg
    39721 atatgttcta ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta
    39781 cctaaagaaa actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg
    39841 acattttgtc ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa
    39901 cggatttgtt tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct
    39961 aatgttttcg cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac
    40021 aaccgatcca gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc
    40081 gcttgttttt tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac
    40141 tctgcttctc gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa
    40201 ttccccgaaa actctcggat ttctccgtct tttacttccc agattttcga tgctagctca
    40261 tctagaaaat agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct
    40321 tcaatcagtc gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc
    40381 ccttgttcat aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc
    40441 catttccgct ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt
    40501 aggtcctttg cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt
    40561 tggattttcc catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta
    40621 ccgacaccat tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca
    40681 atagatagtt ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa
    40741 ttttccatat gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag
    40801 agcagaaaaa tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata
    40861 actatgacta aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa
    40921 atggacatac tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg
    40981 ttattcaaaa gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt
    41041 attaggttac cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct
    41101 tttattcgaa catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta
    41161 ccgcttttcc tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg
    41221 cctctgccaa ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac
    41281 gcgcaactaa catgaattgc atagccgcta agctagaatc gactttgact acatcgttca
    41341 tttcttctct tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga
    41401 cttcttttgg catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt
    41461 aacattgcat atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat
    41521 tgaaacggat caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc
    41581 gccatggttt catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca
    41641 tttcttcgtg tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca
    41701 taattgttga aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt
    41761 atattgcaaa gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca
    41821 tcgctgaaag ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg
    41881 attttcttct gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt
    41941 ctctgacgcg aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct
    42001 tgatcaatct gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt
    42061 ctcatcatga tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg
    42121 attttttgat cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata
    42181 atttcctcct ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa
    42241 aagaaaaaag tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata
    42301 gagaaaagaa attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa
    42361 accaagatta ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt
    42421 acaccgccaa aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg
    42481 gatcagtatg cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt
    42541 gagtaaacgg tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat
    42601 gaaaatctgt ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc
    42661 tctttttaat aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa
    42721 tctctttcct tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat
    42781 agtcgataaa ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg
    42841 atttttgtta aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa
    42901 ggattgtata aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca
    42961 tattcccagc ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt
    43021 tctcctgtat gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca
    43081 tactgtgtca gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca
    43141 gtggcataac gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc
    43201 catgcaccgg aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg
    43261 ttatcttgca atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc
    43321 cattgcccat tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct
    43381 ttaatcccaa atagattgta attaggggca gaagccaaag tactgttccc ccagccactt
    43441 tcaacgatgg cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt
    43501 tgtgcagttg aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca
    43561 taaaaagcat agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc
    43621 aatgtttgcc catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga
    43681 gcttctgctg atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct
    43741 tcggttgaaa ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt
    43801 tcaattcctg tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca
    43861 gctagcactg ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa
    43921 ttctttgctt tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta
    43981 actaaaaaag taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata
    44041 aatttgcttt ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt
    44101 aaattgatgg tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta
    44161 tggattatac ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg
    44221 gcgtttctat cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt
    44281 tttgcgcttc atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag
    44341 tttgaacagc tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac
    44401 gtaatccttc catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag
    44461 gataaacaac atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt
    44521 ccatgattgg aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca
    44581 tattttgcca ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat
    44641 tattgtacaa tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga
    44701 cttgtgcaaa atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt
    44761 cttttattgc ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt
    44821 ctgctaatag cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca
    44881 ctttttctga taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata
    44941 ctcactatct cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt
    45001 tggcagggat tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg
    45061 ctgcaacaat ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga
    45121 tccaagcatt tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat
    45181 cggttagatg gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta
    45241 aacagatgcc acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta
    45301 tactaatatg tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta
    45361 ctttgtcact aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag
    45421 tattcagcgc tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga
    45481 gcggatccgt tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt
    45541 tgctccctcc ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa
    45601 acatacttaa ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg
    45661 aaaaaatgga tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca
    45721 gccgagcagc aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt
    45781 tagaagaaga acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg
    45841 attctggtcg atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg
    45901 cgaacaattt aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa
    45961 ctaaagcaat ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata
    46021 tccagtttca tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat
    46081 tacttgattt tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac
    46141 tgccagccca agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa
    46201 cagtcatcca tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag
    46261 tggacagtca actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa
    46321 catataactt attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag
    46381 ctctagatgg gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc
    46441 cattacaagc ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc
    46501 gtaaattttt agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt
    46561 tcagcttttt aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt
    46621 cttaatttgt tccttccttt tatttctgat aattccagta aattactata attataaaaa
    46681 aagaattgac aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc
    46741 atgtgactag atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt
    46801 ttttcgtgtc tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat
    46861 cttcaaaaag ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt
    46921 agatgacggt ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag
    46981 aaatgatttg atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca
    47041 aatcaagttg caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac
    47101 gatcattgat catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc
    47161 tttgaccgac cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa
    47221 caaactttta agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc
    47281 agtagagtat ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat
    47341 cgcaatccat attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga
    47401 agtgacgatt gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca
    47461 ttcagaacca ttttcactga attattcaag actggtgaat catttacgac tgcttctaca
    47521 acgaacccat gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg
    47581 aaaataccca gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata
    47641 ccagctcgcg atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag
    47701 aagcgcaatc gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat
    47761 ctacaacgaa tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta
    47821 ctagtaggga tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta
    47881 caagctggcg gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca
    47941 ctagggatgt cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa
    48001 gttccagtaa atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa
    48061 tcagttgatc catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg
    48121 attgcggcag ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc
    48181 tttagcggca agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca
    48241 gtgctactgc tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt
    48301 ttgagtttag gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct
    48361 acaggattgc atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat
    48421 attggtaaat ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga
    48481 tttttcccaa tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca
    48541 cgtccagaaa acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc
    48601 tttactggtg taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat
    48661 gtcttgcatg caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca
    48721 gcaggatttg cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt
    48781 gcgaatcagc cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt
    48841 ggctttaact ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat
    48901 ctttcagatg acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc
    48961 aaaatctacg ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca
    49021 cgccttcgtt tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact
    49081 ggcgtacctg gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa
    49141 gtacaattcg ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca
    49201 caaacaaaca ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt
    49261 tacgctatcg caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg
    49321 gaaaaaatga tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca
    49381 atcgctggta cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat
    49441 gctggtatcg aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca
    49501 ttcactttat atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt
    49561 gatcttgccg ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat
    49621 ggcgacaaag tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa
    49681 atcggctctg tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa
    49741 acaaacagga gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt
    49801 tctcctgcca actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt
    49861 tatttgactc ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt
    49921 gttcaaaaac cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt
    49981 tcacaaaatt tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt
    50041 attcctgatc caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata
    50101 ctttttctaa atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg
    50161 ctaaaaacat gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg
    50221 ctgcatcact tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg
    50281 ctaattcttc atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt
    50341 ctactagtac ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc
    50401 catttcctgc agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt
    50461 cattctctac ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca
    50521 ttttttcagt gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa
    50581 aacttgtata agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat
    50641 gttcggcttg acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca
    50701 acgcttcttt cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt
    50761 ctttcagtgc ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa
    50821 ccatcttttt taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta
    50881 aataatgcat atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt
    50941 tataaattac acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa
    51001 ggatgacaac tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat
    51061 attgaaatgt ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa
    51121 acaaatgcag caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt
    51181 acaaaaaccg gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta
    51241 gatgtctatc gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat
    51301 ccaaattgcg tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg
    51361 cagaaaaagg cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata
    51421 gccgttttgg aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa
    51481 tt
//
LOCUS       DAYYHS010000012        45083 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000012 DAYYHS010000000
VERSION     DAYYHS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.012"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHA6A_000698"
     CDS             212..604
                     /locus_tag="ACHA6A_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8968927.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHA6A_000699"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHA6A_000699"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8968928.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHA6A_000700"
     CDS             2698..2943
                     /locus_tag="ACHA6A_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968929.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHA6A_000701"
     CDS             3165..4775
                     /locus_tag="ACHA6A_000701"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8968930.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHA6A_000702"
     CDS             complement(4752..4964)
                     /locus_tag="ACHA6A_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968931.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHA6A_000703"
     CDS             5067..5936
                     /locus_tag="ACHA6A_000703"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8968932.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHA6A_000704"
     CDS             6101..7372
                     /locus_tag="ACHA6A_000704"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8968933.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHA6A_000705"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHA6A_000705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8968934.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHA6A_000706"
     CDS             8763..9026
                     /locus_tag="ACHA6A_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8968935.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHA6A_000707"
     CDS             9471..9920
                     /locus_tag="ACHA6A_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8968936.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHA6A_000708"
     CDS             9977..11395
                     /locus_tag="ACHA6A_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8968937.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHA6A_000709"
     CDS             11395..12255
                     /locus_tag="ACHA6A_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8968938.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHA6A_000710"
     CDS             12429..13202
                     /locus_tag="ACHA6A_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8968939.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHA6A_000711"
     CDS             13349..13714
                     /locus_tag="ACHA6A_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8968940.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHA6A_000712"
     CDS             complement(13862..15070)
                     /locus_tag="ACHA6A_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8968941.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHA6A_000713"
     CDS             complement(15089..15367)
                     /locus_tag="ACHA6A_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968942.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHA6A_000714"
     CDS             15560..16216
                     /locus_tag="ACHA6A_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8968943.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHA6A_000715"
     CDS             complement(16658..17455)
                     /locus_tag="ACHA6A_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8968944.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHA6A_000716"
     CDS             complement(17452..18636)
                     /locus_tag="ACHA6A_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8968945.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHA6A_000717"
     CDS             complement(18661..19908)
                     /locus_tag="ACHA6A_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8968946.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA6A_000718"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA6A_000718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8968947.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHA6A_000719"
     CDS             21888..22202
                     /locus_tag="ACHA6A_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8968948.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHA6A_000720"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHA6A_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8968949.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA6A_000721"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA6A_000721"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8968950.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHA6A_000722"
     CDS             23546..23875
                     /locus_tag="ACHA6A_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8968951.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHA6A_000723"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHA6A_000723"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8968952.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHA6A_000724"
     CDS             24863..25687
                     /locus_tag="ACHA6A_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8968953.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHA6A_000725"
     CDS             25680..26027
                     /locus_tag="ACHA6A_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8968954.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA6A_000726"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA6A_000726"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8968955.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA6A_000727"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA6A_000727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8968956.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA6A_000728"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA6A_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8968957.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA6A_000729"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA6A_000729"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8968958.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHA6A_000730"
     CDS             31684..32451
                     /locus_tag="ACHA6A_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8968959.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHA6A_000731"
     CDS             32441..34483
                     /locus_tag="ACHA6A_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8968960.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACHA6A_000732"
     CDS             34764..34913
                     /locus_tag="ACHA6A_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8968961.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACHA6A_000733"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACHA6A_000733"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8968962.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACHA6A_000734"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACHA6A_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8968963.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACHA6A_000735"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACHA6A_000735"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8968964.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACHA6A_000736"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACHA6A_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8968965.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACHA6A_000737"
     CDS             39330..40412
                     /locus_tag="ACHA6A_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8968966.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACHA6A_000738"
     CDS             40412..41215
                     /locus_tag="ACHA6A_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8968967.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACHA6A_000739"
     CDS             41285..42025
                     /locus_tag="ACHA6A_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8968968.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACHA6A_000740"
     CDS             42036..43040
                     /locus_tag="ACHA6A_000740"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8968969.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="ACHA6A_000741"
     CDS             complement(43085..44929)
                     /locus_tag="ACHA6A_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8968970.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14518 a         7846 c         9369 g        13350 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaa
//
LOCUS       DAYYHS010000013        41822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000013 DAYYHS010000000
VERSION     DAYYHS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.013"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..2824)
                     /gene="ileS"
                     /locus_tag="ACHA6A_000742"
     CDS             complement(26..2824)
                     /gene="ileS"
                     /locus_tag="ACHA6A_000742"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8968971.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(3086..3793)
                     /locus_tag="ACHA6A_000743"
     CDS             complement(3086..3793)
                     /locus_tag="ACHA6A_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8968972.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(3814..4596)
                     /locus_tag="ACHA6A_000744"
     CDS             complement(3814..4596)
                     /locus_tag="ACHA6A_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8968973.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(4645..4866)
                     /locus_tag="ACHA6A_000745"
     CDS             complement(4645..4866)
                     /locus_tag="ACHA6A_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8968974.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(4947..5546)
                     /locus_tag="ACHA6A_000746"
     CDS             complement(4947..5546)
                     /locus_tag="ACHA6A_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8968975.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(5560..6237)
                     /locus_tag="ACHA6A_000747"
     CDS             complement(5560..6237)
                     /locus_tag="ACHA6A_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8968976.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(6254..7495)
                     /gene="ftsZ"
                     /locus_tag="ACHA6A_000748"
     CDS             complement(6254..7495)
                     /gene="ftsZ"
                     /locus_tag="ACHA6A_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8968977.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(7518..8843)
                     /gene="ftsA"
                     /locus_tag="ACHA6A_000749"
     CDS             complement(7518..8843)
                     /gene="ftsA"
                     /locus_tag="ACHA6A_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8968978.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(8999..10219)
                     /locus_tag="ACHA6A_000750"
     CDS             complement(8999..10219)
                     /locus_tag="ACHA6A_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8968979.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(10234..11322)
                     /gene="murG"
                     /locus_tag="ACHA6A_000751"
     CDS             complement(10234..11322)
                     /gene="murG"
                     /locus_tag="ACHA6A_000751"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8968980.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(11348..12709)
                     /gene="murD"
                     /locus_tag="ACHA6A_000752"
     CDS             complement(11348..12709)
                     /gene="murD"
                     /locus_tag="ACHA6A_000752"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8968981.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(12723..13685)
                     /gene="mraY"
                     /locus_tag="ACHA6A_000753"
     CDS             complement(12723..13685)
                     /gene="mraY"
                     /locus_tag="ACHA6A_000753"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8968982.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(13711..15903)
                     /locus_tag="ACHA6A_000754"
     CDS             complement(13711..15903)
                     /locus_tag="ACHA6A_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8968983.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(15904..16305)
                     /gene="ftsL"
                     /locus_tag="ACHA6A_000755"
     CDS             complement(15904..16305)
                     /gene="ftsL"
                     /locus_tag="ACHA6A_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8968984.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(16310..17269)
                     /gene="rsmH"
                     /locus_tag="ACHA6A_000756"
     CDS             complement(16310..17269)
                     /gene="rsmH"
                     /locus_tag="ACHA6A_000756"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8968985.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(17290..17721)
                     /gene="mraZ"
                     /locus_tag="ACHA6A_000757"
     CDS             complement(17290..17721)
                     /gene="mraZ"
                     /locus_tag="ACHA6A_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8968986.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(17891..18259)
                     /locus_tag="ACHA6A_000758"
     CDS             complement(17891..18259)
                     /locus_tag="ACHA6A_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8968987.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18430..19386
                     /locus_tag="ACHA6A_000759"
     CDS             18430..19386
                     /locus_tag="ACHA6A_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8968988.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            19459..19575
                     /locus_tag="ACHA6A_000760"
     CDS             19459..19575
                     /locus_tag="ACHA6A_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8968989.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(19630..21312)
                     /gene="recN"
                     /locus_tag="ACHA6A_000761"
     CDS             complement(19630..21312)
                     /gene="recN"
                     /locus_tag="ACHA6A_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8968990.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(21330..21779)
                     /locus_tag="ACHA6A_000762"
     CDS             complement(21330..21779)
                     /locus_tag="ACHA6A_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8968991.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(21886..22704)
                     /locus_tag="ACHA6A_000763"
     CDS             complement(21886..22704)
                     /locus_tag="ACHA6A_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8968992.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(22713..23603)
                     /locus_tag="ACHA6A_000764"
     CDS             complement(22713..23603)
                     /locus_tag="ACHA6A_000764"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8968993.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(23605..23829)
                     /locus_tag="ACHA6A_000765"
     CDS             complement(23605..23829)
                     /locus_tag="ACHA6A_000765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8968994.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(23834..25171)
                     /gene="xseA"
                     /locus_tag="ACHA6A_000766"
     CDS             complement(23834..25171)
                     /gene="xseA"
                     /locus_tag="ACHA6A_000766"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8968995.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(25172..26026)
                     /locus_tag="ACHA6A_000767"
     CDS             complement(25172..26026)
                     /locus_tag="ACHA6A_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8968996.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(26123..26572)
                     /gene="nusB"
                     /locus_tag="ACHA6A_000768"
     CDS             complement(26123..26572)
                     /gene="nusB"
                     /locus_tag="ACHA6A_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8968997.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(26565..26990)
                     /locus_tag="ACHA6A_000769"
     CDS             complement(26565..26990)
                     /locus_tag="ACHA6A_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8968998.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(27236..28300)
                     /locus_tag="ACHA6A_000770"
     CDS             complement(27236..28300)
                     /locus_tag="ACHA6A_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8968999.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(28472..28717)
                     /locus_tag="ACHA6A_000771"
     CDS             complement(28472..28717)
                     /locus_tag="ACHA6A_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8969000.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(28856..29230)
                     /locus_tag="ACHA6A_000772"
     CDS             complement(28856..29230)
                     /locus_tag="ACHA6A_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8969001.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(29313..30227)
                     /locus_tag="ACHA6A_000773"
     CDS             complement(29313..30227)
                     /locus_tag="ACHA6A_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8969002.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(30248..31051)
                     /locus_tag="ACHA6A_000774"
     CDS             complement(30248..31051)
                     /locus_tag="ACHA6A_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8969003.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(31084..32073)
                     /locus_tag="ACHA6A_000775"
     CDS             complement(31084..32073)
                     /locus_tag="ACHA6A_000775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8969004.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(32176..32667)
                     /locus_tag="ACHA6A_000776"
     CDS             complement(32176..32667)
                     /locus_tag="ACHA6A_000776"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8969005.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(32858..35665)
                     /locus_tag="ACHA6A_000777"
     CDS             complement(32858..35665)
                     /locus_tag="ACHA6A_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8969006.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(36099..36392)
                     /gene="rpmA"
                     /locus_tag="ACHA6A_000778"
     CDS             complement(36099..36392)
                     /gene="rpmA"
                     /locus_tag="ACHA6A_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8969007.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(36405..36749)
                     /locus_tag="ACHA6A_000779"
     CDS             complement(36405..36749)
                     /locus_tag="ACHA6A_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8969008.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(36776..37084)
                     /gene="rplU"
                     /locus_tag="ACHA6A_000780"
     CDS             complement(36776..37084)
                     /gene="rplU"
                     /locus_tag="ACHA6A_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8969009.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(37104..37180)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            37366..38007
                     /locus_tag="ACHA6A_000781"
     CDS             37366..38007
                     /locus_tag="ACHA6A_000781"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8969010.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(38069..39700)
                     /locus_tag="ACHA6A_000782"
     CDS             complement(38069..39700)
                     /locus_tag="ACHA6A_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8969011.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(39823..40182)
                     /locus_tag="ACHA6A_000783"
     CDS             complement(39823..40182)
                     /locus_tag="ACHA6A_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8969012.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(40341..40414)
                     /locus_tag="ACHA6A_000784"
     tRNA            complement(40341..40414)
                     /locus_tag="ACHA6A_000784"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40378..40380),aa:Arg,seq:tct)
     gene            complement(40723..41406)
                     /locus_tag="ACHA6A_000785"
     CDS             complement(40723..41406)
                     /locus_tag="ACHA6A_000785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8969013.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(41534..>41822)
                     /locus_tag="ACHA6A_000786"
     CDS             complement(41534..>41822)
                     /locus_tag="ACHA6A_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HFM8969014.1"
                     /translation="KKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIF
                     QIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT        11710 a         9164 c         6997 g        13951 t
ORIGIN      
        1 aacatgtcac accccctttt cccttttact tttcttcgaa tccttcagct acagcttcag
       61 gatagaagtc ttcaacgatt tttgcacaac gaccgcatag atgaggaagt ttttcatcta
      121 ctcctacatc tttacgaact tggcgacagc gatcgcaagt ttccccgtct gctttttcca
      181 ctgtaatagc aacatcatca aataccatag cgttatctgg cacttgttca tctgctgcca
      241 taacttcaaa gtagtctggt gaaacgatca ataattgtgc aatatctgca tccaaagctt
      301 ttaacatttc acgaacttgt tgttttggat aaacagttag ttttgcttcc aaagatttac
      361 caatcaattt gctgtttctt gcttcttcca atgcttttaa gacattatca cggaagtcca
      421 tgaaagcagt ccaggtatcc atcaattctt cttggtttgc aaactcttca taaccaggaa
      481 actcgcttaa ctgaacgtac tcctcttctt ctttcaggaa tgaccagatt tcttctgctg
      541 tatgcggaat gataggagtc aataattttg tcaaagctac agctgtttga tagaaaacag
      601 tttgcatgca acgacgttga taatcatctt ctgcttcaat ataaactaca tctttagcaa
      661 aatccaggta gaatgacgat agatcaaccg tcaagaagtt cataactgta cggtaaatat
      721 gcatgaagtt atatttgtcg tacccttctt ctcggatttc tttgattact tggttcaatc
      781 gaacggtcat atatttgtct acagaacgta aatcattgta cgaaacagta tcttttttag
      841 gatcgaaatc ttctgtattc gctaataaga aacgcatcgt atttctgatt ttacgatata
      901 cttctgccac ttgacttaag atatccatag aaacacgaac atctgcttca taatctacac
      961 tagataccca taagcgaagg atatctgcac caaattgttt gatcactttt tctggtgcaa
     1021 tgatgtttcc taaagattta ctcatcttac ggccttcacc atctaaagca aatccttgag
     1081 aaataactga tttgtatggt gctacaccat tgatagccac actagtcgtg atacttgagt
     1141 tgaaccagcc acgatattgg tctgatcctt ctaaatacat atctgctggg aatgataatt
     1201 ctggtcgctg acgtaaaaca gcttcatgag aagaaccaga atcaaaccag acatccatga
     1261 tatctttttc ttttgtaaat tcgccattag gagaacctgg gtgggtaaat ccttcaggaa
     1321 gaagttcttt cgcttctttt tcaaaccaga taattgatcc atgttcagca aataattttg
     1381 ccacatgttc gattgtttct ggtgtgatga ttgcttctcc attttcagca tagaaaatcg
     1441 gaagtggtac tccccatgca cgctgacggg aaattaccca atcgccgcgg tcacggatca
     1501 tattatacaa acgtgtttta ccccatggga taacccactc tactttttct acttcatcca
     1561 aaatattctg acggaattta tcgatagatg caaaccattg tggagttgcc cgatagatca
     1621 caggcttttt cgtccgccaa tcgtgaggat aactatgagt aaagaaatct agcttcagca
     1681 aagctccttt ttcttttaat agatcagtga tcataggatt tgctttgtca taaaagatac
     1741 cttcaaatcc aggtgcttct tctgtaaaca atccttttga gtctacaggt gacactacag
     1801 atagtttata tttgctacca gcgatatagt catcttcccc atgtcctgga gcagtatgga
     1861 ctaatccagt accagcgtct aaagtaacat gatcgcctaa aatcacaagt gattcacgtt
     1921 catagaatgg atgacttgca gtcatatatt ccatttcttg tccagcaaat tcttttagaa
     1981 ctgttacatt ttcccaaccg atttctgatt ggactgtttc tagcaaatct ttcgctacga
     2041 caaacttacg gccatcggct tccaccaata catacgtaaa ttttggattt acagcaatac
     2101 cttggtttgc tggcaatgtc catggtgttg tcgtccaaat gacaaaagct gtatcttcat
     2161 caagaatatc tttgccgtct ttgacattga aagctacaaa aatagatgga gattttacat
     2221 ctttatattc aatttcagct tccgctaaag aagactcact tgacggagac cagtaaattg
     2281 gtttcaatcc tttgtaaata tagccttttt cagccatttt accaaatacg cgtacttgtg
     2341 cagcttcata agaaggatct aatgtaacat aaggatgttc ccaatctcct gcaactccta
     2401 ggcgtttgaa atctgctcgt tgcttatcta cttgggaaag tgcatattca cgacattttt
     2461 ccaagtattc tgccatggac atttctttac gtttcacgcc tttattagcc aatacttgtt
     2521 caatcggcaa tccatgtgta tcccatcctg gaacatatgg tgcacgatag ccagacattg
     2581 attttgaacg aatgataatg tctttactga ttttattcaa tgaatgtcct aaatggatat
     2641 taccattggc atatggaggt ccatcatgaa gaacaaagct aggttttcct tcgttcaact
     2701 cttgtctttt ttggtagatt cgattttctt cccattcttt ttgccactcg ccttctcggt
     2761 ttggcaaatt accacgcata gggaattttg tttttcctaa ttgcaaggtt tctttcattt
     2821 tcattcaacc atacactcct ttatttgttt tccggacaca aaaaagcatg ttcagccgca
     2881 agggacgaac atgccgtggt accacccaaa tttagttgca tctatctagt gcaaactctc
     2941 tgtgaccaat aacgaggtca gccgctgctt cttactctaa tttcggaaaa gcaggtctca
     3001 agtagaggat ctttcaccat gcagtgtctt accgaacttc cactctcatc ggctcgcttt
     3061 aagaataaca aagtgctttt ctgttctact cgttttcatt ttcaccaggc acgatcgaga
     3121 tatcaatagc ctgtgtatca aatgccgtca taggtgcttc tatgactcct gtgtctgagt
     3181 ttacatcagt atcattttca ttgtcaagct gttttgctaa aacttctttg atcacttcat
     3241 ggctgtcagt cacgtagctg gagaatggtt gtaaaatttc atcccattcc ggacttttca
     3301 cttgttctaa ttgagactca agcatcaagc tgatacgttg atggaatact cgtgtttttt
     3361 tcttaagatc ttcagtttct gtcgcaagct gtcttgcttt ttctgttgca tctgtcaaga
     3421 tttttcttgc tttttcttct gcatcagttg ttaattgatg tgcacgtttt tcagcgttcg
     3481 caactaactc atctgctttg ttttgtgcag aagtaacgat cacttcggat tccttgcttg
     3541 cacttgtttt tactttatca gccgtatctt gagctacgat gattgattgg ttcaaagcat
     3601 cttttaattc attgaagtac tcaagttttt cttcagaatg tttgactgct ttttctaatt
     3661 ctcgatttct ttgagtcaat tcctcatagt cacgaacaac taaatctaag aaatcatcta
     3721 cttcgtcttg attatatccc cgcatttttg tcgggaatgt cttattttgg atatctaatg
     3781 gagttaaagc catgagtaaa ccaccttttt attttattta cgtaatacac cgaacagtac
     3841 cctgtacttt tcttttttcg tcttcccctc aagttcttgg atctggactc tgccataacc
     3901 tctgactgaa ataatatcta atagttcaag tacaaaatca ggtcttgtat tttctgtcca
     3961 gttcactttt actttaccag cttcgatcag ctgcttggac cgctgtctgg aaatattata
     4021 aatctcagag atcacactgt ctagtcgcat ggaactaact gttcctcttt cttgtgtcca
     4081 gctatctttc ggcataatca tttctgtgta tttgattggt tccaagcgta cagcaacctt
     4141 tccaatcttt gttacttgtg cagcaacaaa atgatccacc tcatttgcaa taaatacttg
     4201 ccagcgatca ccgtccgaga tgatatcgcc aaagtaagag cgtttgatcc ccgttcccac
     4261 taaagttcca aggattttcc catgacttag gctggcaaat ttgctaggat aatggatttc
     4321 atacaatgct gtttcaaaat cttcctctac aggttcgtaa taatcgggga aaatcaaaca
     4381 tctttttctt tctgcctgtt catatccgcc ataaaacatg aaacgcaaat cactgctttg
     4441 tcgaatcagt gtttctaaaa tataggattg tcttgggtca agaaattcgg tcagataggg
     4501 tgcatattgt ccttgaacct gttcaatcca atcgcctacc gaatcgataa atgtacgttc
     4561 atctgcccga aagtgctggt atacatttgc atccaaaatt ttcccctcct atctattctg
     4621 agaataaaga ttttgttgct tcaatcaata aactaaatgg acagttatcc atataacgat
     4681 ctgtgctaaa ccttgtacgg ctaagttcaa gatcaggatt gcccctaaaa ttgtgaaatc
     4741 caagggtcca atcctcaaag gcagacgatc gaacaaactg atatacggtt cgcagatctt
     4801 agctaataat cgtcctagcc ctgattgata cccgcctgga aaccaagaga gcaatgcata
     4861 taccatcaag ataatgctat agaaataggc tgcccgttga agtaaatgaa gtaatagtgc
     4921 aatagtataa atcgtagttc ctcctcttac agatcaaaca aattcgcatc tgcaaaagat
     4981 tgagcggttg aactgtcaat ctccatccca gtcggtgtac aaagaaaaat ctcatctcca
     5041 acacgtttga tatctccatc tagtgcataa actgttcctg ttagaaaatc aacgattctt
     5101 ctcgcttgtt tttcttctac taaatgaaaa ttcactaagg ttgcttctcc agcaatgatt
     5161 cgtttagcaa tcgccatagc ttcagaataa gctcttggtt ccattacagt gatttttcca
     5221 gcaagatttg ctttaggttg tgtagaggtt cttgaagctg ttctttcttt cgctacagtt
     5281 tttgtaggtg attgatgtag ttcaattact tttttctttt cagccggctg aatactttca
     5341 tgcgttacag gacgtgaagt cgccgttttt tgtttttcat gagtattcgc agcagataca
     5401 gaatgagttg attgattctg tgttgtattt tttttagttg gctgtacagc cttttttgtt
     5461 gtttcaggat aatcttggta ttcgtactct tcctcatcag ctaatccgaa aaaattagca
     5521 attgctcctc tgtttaaaag cggcattttt gttcctcctt tattcgtctt tgaatagcgc
     5581 tgttcctaca cggataaatg ttgcaccttc ttcaatcgca atttgaaagt cttgactcat
     5641 ccccatactt aattcactac atggtgcatg ggcataacct tttgcttgaa caagatcacg
     5701 caattctttc agcgtaccaa acactttatg gatttgctca tcagaagctt ctttaggcgc
     5761 catagtcatc aacccaacaa ccttgatttt tgtgcaggca gctaaagatt ccacaaacgg
     5821 taaaagctca tctggtgaaa ttccatgttt tgattcctca ccagaaacat ttacttcaat
     5881 aaaacaggat aattctttct ctaaccgctt ctggatttct tctgctaatc ttaaactgtc
     5941 caacgcatga aagtaatcaa tttcatttac aatcaatttt acctttctgc gctgtaaatt
     6001 accaattaaa tgccacttta tttcaggaaa atcaaataaa tcttgtttct tttctaataa
     6061 tttatctact cggttttctg cacaattcat aacgccagct ctaaccaatt cttcagcttg
     6121 agcggattca acagattttg ttacagctac taatgtaact tctttcggat cacgatgaac
     6181 aagagcacac gaatcctgaa tttcttgctt gattttctgc aagttgtcag caatcatgtg
     6241 ctcttcctct cctttatttt ttacgacgga aaaacggtgg cgtatttaat tcatcatcgc
     6301 catgagattt tgtttcttcg cggttgaatg tatcaaattc ttttttttcg atattgtcaa
     6361 aattggaatc atcaactcta ggacgaacat tttgttcacg tctgatatcc caatcgccaa
     6421 aactgttttc ttcttctgaa gaagttggtt tagcttgatc catatctaat acagtctttt
     6481 gagcaggtga ttgcatttgt gcttgtctag ctggtcggct agacttgcgt tccttttttg
     6541 attcatcgat acctgttgcg atgaccgtca cacggatttc atcgcccatt tcttcattga
     6601 tagatgtacc tagaatgata tttacatcgc cagttgctgc atttgctacg atatctgaag
     6661 catcttgtgc ttcgaacaat gtcatatcca aaccgcctgt gatgttaaga agtacttgtt
     6721 cagctccatc aatagatgtt tcaagcaatg gagaggagat tgcttttttc gttgcttcta
     6781 ctacgcgatc ttctccacta gccacaccga tccccatcaa tgctgtacct tggttttcca
     6841 tcactgtttt cacgtcagca aagtcaaggt taacgtaacc tggcgctgtg atcaaatcag
     6901 agataccttg tacaccttgt cgtaaaacat tatctgcttc acggaaagct tcaagcatcg
     6961 gtgtcttttt atctacaact tctaataaac ggttatttga gataattaat aatgtatcca
     7021 cattttcttt caaacgagcg ataccttctg cagcaaaacg gccacgtttc ggtccttcaa
     7081 aagtgaatgg acgtgtcacg acaccaacag tcaatgcgcc caattctttc gcgatacctg
     7141 caacgattgg tgcagcacct gtaccggttc ctccacccat tcctgcagtg atgaaaatca
     7201 tatctgcacc gtctaaagct tcgcgtaagg attgttcact ttcttctgcc gctttttgtc
     7261 caacttctgg ctgagaacca gcacctaaac cacgagtata tttggggcct aattggataa
     7321 ctgtttcagc ttttgaattt tttaatgctt gcacgtctgt gttggctgta atgaattcaa
     7381 cacctttaac gttttcttca atcattcggt ttacagcatt tccaccgcca ccaccgactc
     7441 cgatgacttt gattactgcg ccgtcattga tattattatc aattgagaat tccatatctt
     7501 attcctccca ggatgaatta atcaaaaata tttgtaaaga aacccttgat tttatttttt
     7561 acattttctc cggactcttt tggttccggc tcatcgtaga ttgtttcttc aggtgcttca
     7621 tagctctcat atgtgtttgt tgtttcttgt tcaactacca tttgacgagc tgctgaggtt
     7681 tcacccgtaa cagcaatttt tgccaattga tacacttcac tcaagtttgc tgaataatcc
     7741 acgatactta ttacatttgt aaataccggg tttctcagcc ccatgtgatt tggaacatac
     7801 aattttacat ttacgccgaa gatttcttgt gccaagtcca caacaccagg aagactagca
     7861 gctccgcctg tcaatacgac gccacctggc aactctaatg cttcgatttg gtccaatgca
     7921 tcttttgctt tgttgaaaat ctgttccatt cgagctgaaa taatttctga caagtagcgt
     7981 tcatcgactt tcactggttc tgactgacca atcacatcta ctggaaattc ttcatctggt
     8041 gatgtccgtt ctgggtatgc atccccgtag tttattttca acgcttctgc attgttaaat
     8101 gacgtattta atacaataga gatatcttta gtgacaaatt cgccaccttc ttggtcaaga
     8161 ttggtaaact tcaattgctt atcatgcata acagccgttg ttgtttggcc gccacccata
     8221 tcaatgacga tcgtaccaaa atctttttct ccgtcagaaa gaattgattc agtcagcgct
     8281 aaaggagtaa tcaccatttc gttgacgatt aatccggcat tttccacgca tttacgtatg
     8341 ttatggatga tcgttttagg tcctgtaaac aacagaccgt acatttctaa tcttacgccg
     8401 atcattccac gaggatcttt gattccctcg aacccatcga cagtaaaatc ttgtggcaat
     8461 attgacacga tttgacgttc aggaggtatt gaacgaacaa gggcagctga agccacattt
     8521 ctgacatctt catcggtgat ttcctttgaa tctccgttaa ctgcaatcat tccctggcaa
     8581 ttttctactt caagcatgtt tgctggcaaa ccgacactta cgcctttgat ctggatacct
     8641 gctttttctt ctgcttgtcg tactgctcgt tgtatcgctt ggaccgtttt atcgatatca
     8701 acgataatgc ctcggttaat cccttctgat tttgcgtttc ctactccaat aatattcatt
     8761 tggctgtcga tatattcagc cacgacaact ttaacagatg tcgttccaat atcaaggcct
     8821 acatacattc ctgtttttgc catgaatggg attccctcct acttactatt ccatatacaa
     8881 gcgaacaact agcgtccgtt ttaaaatata cttattaata gatatattca ctgcttccaa
     8941 ttctaccata aattactggt ttttagaaag tacattctta ctttttaaag ttgttttatc
     9001 aattttcatt taaacttgac ggagttgtac ttggattatc ttctatttga cttgtttcac
     9061 ttgaatcatt tgaatcagat gatccgtttg tgctagacga tccaggtgtt tgtgcttttt
     9121 gcgtttctgc ctcttcactt gattcgtttg tttgatccgc attttcttca ctttcagttg
     9181 tttcttccga ttggcccgat tcttccgttt cttgagtgct accgtaagga tgcgagaaga
     9241 taccaacttc catgtcgatc acaccttttt catccatatc tttggctact tgcgaataat
     9301 atttcatttg gttcgcaaga ttattgatat ttacaataac ttgattgccg tcattcatat
     9361 tcaattggat cagattttta ttgcttgctc ttggcgtgta cttgatttca gaaattgctt
     9421 tttgtaattc tgatgataac tgattgtatt gctttgttaa ttctttgatt tttgcagagt
     9481 ctttgaactc ttccaagata gggagatttt ttgtaggatt tgctactttt tcatctaata
     9541 ctgttccatt ttcaatcact gggtagtatt gattgttttt tgcgatcaaa gcaatctctt
     9601 tatattctgt tacatccaaa tcgaattcat tcatcccttt gaaatgtata tttgctgttt
     9661 cgattcttgg ctgtgctttt ttaaggcgat cgatataagt agatcgatga aaatactgtc
     9721 cccaaacatt ttctcctaat gacaattttg tatcagagat gatttcttgg ctgttgactg
     9781 tcttattgcc tgatactgca actgcttgta atttgctata aggagaaaca taatagatca
     9841 aaaaaatcag cggtataccc aatacagtga tgatcaaagc taaccgacga aatagtactt
     9901 tgtgccgttg actttttaaa ttgggcagcc ggttcaaaaa agaaccgtta tatggtccac
     9961 cgtttttctt ttgcttgatc tcttcaaaaa attcgatttt tttctcggac tggctttctt
    10021 cttgctgttc ctcttgaacc gttttttctt cttcagaatc tgaaaggggt gtatctaatg
    10081 ttatgtcttc tttttttggc tcttggtttt tcatctcttg ttctgccaga tattttcggt
    10141 tagccttttg ccaaggagtc aaattgtttt catcagaaga cggatgagac aatttttttg
    10201 gttctttttt cttactcact aagaccaccg cctttatttt ttgattattt gttttactaa
    10261 gtcataaaga cgttttgaag catcagggat tccttgttct ttagaagctc ggcacatttg
    10321 cttttgcaat tcttcatcac ccatgatttc gttgactgtc tgacttagtg attcacctgt
    10381 tagttcatta tctgcaatca tttttgctgc tcctgcgtga acaagactca ttgcattttt
    10441 agtctgatga tcattcgtaa catatgggct gggaaccaac actgccggta accccagagc
    10501 tgtgaattct gcgatcgaag ttgcaccagc acgccccacc agcagatcac tggaagccat
    10561 gacctctgcc atatttttga tataaggctg gacactaata ttttgtattt caggatttat
    10621 tttatttagg atctcttcat aatatctatc tcctgaagca tacaaaactt ggtaagaaga
    10681 aggaatatat tcacttaaaa aaactgatac tgcttgattg attttaagtg ctccttgact
    10741 accgccaaaa atcaagactg ttttcttttc tggatccaaa tcaaatgttt tcaaaatatc
    10801 tgatttttta gtatctgcta cttcttgtgc tcgaggatta cctacaagaa ctgctttgtt
    10861 ttcaggaaaa aaggaagccg catctttaaa tgaaagagcg atccgatcca catatcggct
    10921 taaaaattta ttggtaacac ctggtacact gttttgttca tggataactg ttggaatacc
    10981 cattttggct gcagcgtaca ctaccgctcc ggaaacatat cctccagtac cgataacgat
    11041 atctggttga aaatcactga ggattttttt tgcttgacgg atactcttaa gaaacaatcg
    11101 gaccgtcttg atgttatgca tggaaatttt tcgtttaaat ccttgaatct ccaaggtatg
    11161 gaaaggcatt cctgtatcag ggacgatttt gttttctaga cctcgttttg ctcccacata
    11221 catgaattca gcattcggtt cttgagtctt tacataattg acaaaggcaa gagctggata
    11281 aatatgtccc cctgttcctc cgcctgtaac caaaattttc attttttatc gctcgcttct
    11341 actttttcta ttttaatcgg ttcacagctt ccatgaatct gtttcctcta gtttcaaaat
    11401 ttgggtattg atcccagctg gcattcgctg gtgataataa aactacatca ccatctgtac
    11461 tgttttctaa tgctaaagga actgcttgtt ctacagaatc ggctgttact attgtctcga
    11521 tgcctgcttt ttttccagca tccatcaact tttcttttgt ttggccaaat acgaccattg
    11581 ctttgattcc ttttaatgaa ggaattaatt catcaaatgt atttcctctg tctaatccgc
    11641 cagccaataa cacgacctta gacgtttcaa atccacttag agccatttta gtcgctaaaa
    11701 tattggttgc ttttgaatca ttataaaatt ttctgccttg gatttctcct acatattgcg
    11761 tacgatgtgg tacgccatgg aaaaaatgca acgtttctcg tatagcttca tttgatatac
    11821 catacaattt tgctacagaa atagcagcta gtgcattttc tacattatgc ttacctggta
    11881 cgccaagttc agatatttcc attatttttt cttttttata gtatattgag taatcgtcag
    11941 catacacacc gtcttccaac acttcttttg tcgagaaagg cagtacgcgt gcttttgttc
    12001 tttgcgctaa ttcttgtagt tctgactgat tccagttaag gatcagataa tctttttctg
    12061 tcatgttttt ttgaaggttc catttagctt tgacatattc ttttcttgta ccatgataat
    12121 caatatgtgc ttcatagata ttggtaatca ccgctatatg agggcggaaa tctgtgattc
    12181 ccatgagttg gaagctagac aattccatca caattttatc ttctgatttt gcttcttgcg
    12241 ctacaccact tgctggatac ccaatattac ccgctaaacg tgcaattcct tgatctgctc
    12301 ctgcattcag tagtagtcct gtcatcgttg ttgtcgttgt ttttccgttc gttcctgtaa
    12361 tcccaataat tgggcattcc gctacttcgt aagctagctc aacttccgta attaccggaa
    12421 tacccatttc ttgggctttt gctactaaag gatgactgta gggaatccct ggatttttga
    12481 ccatcaatga aaattcttca tccaacaatt cgattggatg actacctgtt attactttta
    12541 tccctaaaga aagcagctct tgtgcttccg gattttcgtc aaaaggtttt ccatcattaa
    12601 cggtgacaag tgcgcccaat tcatgtaata atttggccgc acttactccg ctttttgcga
    12661 gtcctaagac aagcactttc ttattttcat atgttgatat ttttttcatt tcccgtcatc
    12721 tcctaccaaa cgatccataa tgtgatcaac gaagtcacaa tgctgatcaa ccaaaaaact
    12781 gtatcaatct tccactccga ccatccgcac atttcaaagt gatgatgaat tggagacatt
    12841 ttaaaaattc gttttcctgt aagcttaaag gaagtaactt gaagcatcac acttgctgtt
    12901 tccataacat agatcaaacc aatcaaaagc aatgtccatt cttggtttag cattatagaa
    12961 attgccgcta aaagtccgcc caacgctaat gaaccgacat ctcccataaa aatcttggca
    13021 ggttttctgt tgtagacgaa gaaacccagt aaacctccta aaacacttaa gcaaatgatt
    13081 aatacatcat actgatgctg atgccaagca attagaccgt aagctgcaaa agagatactt
    13141 cctaagccag caactaatcc atcgatgcca tctgtcagat ttactgcatt tgaaaatcca
    13201 acaagccaga agagagcgaa aataccataa atgattccta aaggtagatc tagtccgaag
    13261 aaattcaata cacctggata accttcagaa cggtaaacaa gataaaagat gattccccct
    13321 agaatctgtc caatcaattt ctgcttcgag ttcagtccca tgtttctttt cttgaatatt
    13381 tttataaaat catcaagaaa gccgattact ccgtataacg ctaatacaaa aagcaagatg
    13441 aaaagagtag gagttagctg tttttgccaa gctccaaccc aaattcctgt caaaatagag
    13501 ccgatcagaa acactaagcc gcccattgta ggagttccgg ctttgctatt gtgccacttt
    13561 ggtccctcct cacgaatcgc ctgaccttgc tttttcattt gaaaataacc aataaatagc
    13621 ggcattgcgg caattgtcat tgcacaactg ctgacaatgg gaatcaatgc ttgtgtccat
    13681 tccatgctct tttctctcct atttctcttc ttattcttct aaagtaaaac taagtttgtc
    13741 ttcagtgatt ttttcatatg gagcaagttc ttgtttgaca caataaccat ctccatcaaa
    13801 actaacttca ataccgagga ttttccccag cttcatgata tctgcttttg accatcctgt
    13861 tacgtcaggc atcagcttat caccaccagt atataggatc agtttttctc cagagatcaa
    13921 ctgatcaccg ttggcagtag attgtttttg tactttttta ccattaccga tcactacagg
    13981 ttgcagtcca cttttttgag catcagctgc tgcaacgtcc gcttcaagat tacgataatc
    14041 ggctacggat actttttctg tttttgtttc tgtatctgaa tcttcttcag tttctttaaa
    14101 atccattgcc cgtttcatca atggatttgc gattttagcc aatgccattc gatcatatgt
    14161 cttagggtgt ttcattgtta gatacaagac atagtctgga tcttcagaag gaaccatctc
    14221 cacaatcgaa tacaggtagg ctgtatctcc tgtttgatat ccccctgtat cagaagcgat
    14281 ttgggctgtt ccgttttttg ccgatacatt gtatccaggt acactatata caccataagc
    14341 actaccgtag ttttcgcttt cgacaacatc tctcatatat tcacggactt tttctgttgt
    14401 ttcttttgaa aaaggttgcc ctagaacttc tgtttgagtt gttcgctctt ctccagtttg
    14461 gggatccacc actttgctga tatatcgagg ttgaatcatt gtaccgttat tcgcaataga
    14521 agtgaaagct ttcatcattt ggaaattcgt cacacctacc gcttgtccat aagcactcat
    14581 tgctcggtcg acaatattta atgtaggtaa agcaccgttt acttcatcat ctaatccaga
    14641 atgagtactt tgtccaaatc ccagtttttg taaatagtta taccatcgtc cgcctaatcg
    14701 ctgttccagt atgaccatcc cgacgttact tgaccatgaa agagcttgac gcatggtcag
    14761 cacacctttt tcaccaaagt catggtcatt gatcgtagca tcttctactt ggatttttcc
    14821 agattggaag gtttcattct cgttgaattc tccttcttcg atagcagctg cagtagtgaa
    14881 aactttcatg gttgatcctg gttcataact atcttgtacc aagaaattac gccagatagc
    14941 atcttttcca gtcaatcctt ccattgtttc aggattgaaa gtaggtcgtt gtcccattgc
    15001 caagatttct ccggtttttg ctttcatcag gactgctgtc aattcttctg gctgatattc
    15061 ttcatttact tgatccatca gtgtttctaa ataactttgt agtctgctgt ctaaagtcgt
    15121 gtagatatct tgtccgtcga cagctttttc ttcttctgcc actgtacctg gcagaggatt
    15181 ttgaaaatta tctttttgat aaataatctt accatcttta ccgctcaaga tatcattgta
    15241 agcagactcc aaacctagtt taccaactaa gccattctca tctttgtctg gaactgcgta
    15301 tccaataaaa tgagaagaaa agacgccatt tggatacatc cgagcctgat ggtcgacaaa
    15361 ataaagacca gcgacacctt cttttttcat gtccgcttcg atgttttgct tggtttcttg
    15421 ggagatattt ttcccatagc taccaaattc aacttgatac agctcttttt ttgcgccgtc
    15481 ctctaaaata ttcagtgcat cttttttgtc tatagaaaga ttcttatgca ggatttctgc
    15541 tattttatca aaattctttt cttcaacata cagtttttta tccccagagg tatatgtttt
    15601 tgagagtatc gctttaattg aataagagga ggcatcttct gcgagcgcca caccgttacg
    15661 atcataaatc gttcctcttt ttgcttttac tacttcactt ccttggtaca actgttttgt
    15721 tttttcagct aatgaggtac cagcaacatg acctccaata acgatatacg agaaacggac
    15781 ggcaaataaa aagaacaatc caatactcgt agcaaataaa atgataccta cttttttgcg
    15841 attgttcatc ggatttagat ttttcttttc cataaaccgc cgaattttat ttttaagact
    15901 catttacttc actttcctta aattgtcgtc attaatagac aatccttttt cttctgcgat
    15961 ttgtttaatc cgttcagtac gagaaagttc gtttttttct tgttctaaac tcgttttttc
    16021 ttgcttttta tcattgattt ctgtttgtat tgacgtgatt tctttttcag ctccgctgat
    16081 atttgtgcgc agcataactg ttaaaacaca taaagcaaca atagcaatcg ccacgcttgc
    16141 cacagcaatt ttttccaaag cagaaatctg ctttaatcgt tttgccggtg attgtggaag
    16201 aatgatttct tgattcgaag aagaatcagg catttgttgt tccggctgat ccaccaattc
    16261 aggtaaatca tattcgtaat cttgcatttt ctttaattct gccatcattc tactcctctt
    16321 tcattcttat tttttctgca atacgtaact ttgcactgcg tgatcgattg ttttctgtta
    16381 gctcttcctc tgaaggaagg attggtttac gagtaatgac tttcagttct ggttggaatt
    16441 cgtcaggtat gacaggaagt cccggtggta gatcttgtac cgtactatat tctttaaaca
    16501 tacttttgac aattctgtct tccaatgaat ggaatgtgat tacgctgatt cttccattga
    16561 ctttcaataa ccggattgcc tgttctaaag aggcctcttc ggcaccgagt tcgtcattta
    16621 ctgcaatccg cacagcttga aaaattcttt ttgcaggatg cccgcctttt cttcttgcgg
    16681 gagctgggat gacttgttta atgatttcga caagctcacc agttgtttgg attggttttg
    16741 tttttcgtac tcgttcgatt tcacgagcaa tttgtttgga aaatttctcc tcgccatagc
    16801 ggaagaaaat ctttactaat tcatggtagg aatattcatt aattacgtga taagcggata
    16861 aaggagctga ctgatccatg cgcatatcca acggtgcatc ttgatgataa ctaaatccac
    16921 gttctgcttc atctagctga ggagaggaaa cccctagatc atacaaaatg ccgtctacct
    16981 tatctacatg ctcactcaag cgttcttcta attctctgaa gttcgctttg ataaatgtga
    17041 ccataccttg ttcgacataa gaagctaaac gcagcttggc gtgatcgatt gctttttgat
    17101 cttggtcaaa cgcatacaga tgccctttag aacttagttg gcttaataaa tattcactat
    17161 gtccggcacc gccgagtgta cagtctacat aaacgccatc cggtttgatg ttcaatccat
    17221 ctaccgtttc tttcaacatg actgtatagt gttgaaatgt ttcagtcatt agtcagcctc
    17281 ttcctattct tatagtccga aatcaatcat actttcagca atttcgtcaa agttctcttc
    17341 tgctgctgca gaaaaatctt gccatcgtgt ctcatcccaa atttcaattc ggttagatac
    17401 accgatgact acgcaacctt ttgtcaaagc tgcataattt cttaacgtgc tgggtatatt
    17461 gattcgtcct tgtttatcaa gttcgcattc agtggcagcc gaatagaaga atcgcacaaa
    17521 agtacgtgcg tcttttttag ccaacggcat ttcgttcagt ttagcttcca gattttccca
    17581 ttcgctcatc ggatagccga aaagacagcc atccaatccg cgtgtcagaa caaacttttc
    17641 gccaagttct tcacgaagtt ttgagggcac gattaaacgt ccttttgcat caatattatg
    17701 ttggaattca cccatcaaca ttgaaagccc cccattcctc cactaactaa aattagtcta
    17761 ccacaatccc ccactttcta ccaccttttc tgggaaatcc gggaacaact ttttttattc
    17821 ataaacattt ttttaaaaat caagctgcaa aaagcctttt aaaagacttt ttacagcttt
    17881 tctatttttt tcataggtaa gaaagtatgt tcacaatgat caagacagcg tacaagacga
    17941 ttgtcaacag aaaaaccaaa cgccaagtca tcttgaaata acgcggataa ataatttctt
    18001 cataataata tgcttggaaa acagcaatac aaattcccat tagcaaaaca gaaataaaga
    18061 aaaacggtaa gatagaaatt gtccctgtat ttctagacaa tgcatgcata ccgccccaca
    18121 aaaaaggaat cgccaaatca ggggctttta ttttaaaacg acgatttaac gaaaataatt
    18181 ttacaagaaa ttggcaacca aacaaaacga ttaccgggta aatataccaa aacaagataa
    18241 gtggtgtgaa tgtgcccatg aacgtaaact ccttttttat cgttgcttca agtatactgt
    18301 atatacttta cacaactgtt tcattttatt taaatttatg cagttatatt ctatatctta
    18361 caaacacatt ccatgtattc caattcccta tccgttataa taaaagaaac aggcaacagg
    18421 aggaaaatca tgatcaaata ttttacatta gataatgaga aactgcgtaa taccggaaag
    18481 gaaactgacc aaacgatctg gtattgtgta gaacgtccta atcaagaaga gattcatcag
    18541 ctgacacatc aatttgaaat tccgtcagat tacatcactt ccatattgga cgatgcagaa
    18601 aattcccgta cagaagaatt caatcaatca aagttgacaa agccagcgtt attattgtta
    18661 caatatcctt ttgctaaaac aagtcctagc ggttattttc aagtggatac atacccgctc
    18721 tctattattt tgacgcccaa aaagaagctt atcacaatta cgaatcatga accactgttt
    18781 ttaaagaaag tctttagcca gacctttcca aaaagtgata tttcgcctaa tctcaatatc
    18841 ttctttcaat tcatttggca ggtcacactc tcttacaaca gttatttagc tacgcttcaa
    18901 tctcaatgcg atactttgga agccaaactt caagtatcca ctgaaaacaa acaactttat
    18961 caaatcatgg atatccaaaa aagcctcgtt tatttcagag aagcgacttc tgctaattta
    19021 gatgcattga aagaactagc gcaaaataaa cttttccaag aaaatcatgc tttgaaaaat
    19081 catttacgtg atgtattggt ggaaacagag caggcaatga ctacttctcg cattcaatta
    19141 aaactagtag aacaaatgaa tcagactttt tcagcaatcg tatcgaataa tctgaataat
    19201 attatgaaaa ttttaacttc tttaacgatt attttaacta taccgacaat cgtcggcagt
    19261 atctatggaa tgaacattaa gttacctatt gctgaaaggg atgatgcctt tatatggcta
    19321 gttctattaa cattatttat ttgtatcgta actacctatt atttaaaaaa agggaagttt
    19381 ttataaaaaa gccctcttgt ggaaatggaa aaaagattgt acacttgaat caactaatta
    19441 atgacgaggt tgaaaacaat gcgtgaaaaa aaacaaacaa gtacttttgc taaagtaaca
    19501 aaatttgtca tttggacaat gctgatttta acgattggat ctgttgtatt gactgctgta
    19561 atgagtgtca tgtaaaaata aaatatagct atcagctgct gaaaaggacc tgaaccactg
    19621 cgaggtggct caggtccttt ttgcgagttt caatagttct tttgcatgtt cgagtgtgag
    19681 ttttgtaatc tcacttccag ccagcatacg tgcaatttct tcttctcttt cttttgtttt
    19741 caactctgaa acagaagttt gcgtacgtcc atcaatcact gctttcacaa tataatattg
    19801 atagtcggcg actgcagcaa cttgaggcag atgagtgatg cacaaaactt gagagtattt
    19861 cgatatttga gaaattttat ctgcgatggc ctgtgcaact cttccgctca cacctgtatc
    19921 cacttcatca aaaataatac ttgtcacacc ttgctcagaa gaaaaaatag ttttcaaagc
    19981 aagcaacatc cgagacagtt cgccaccaga agcgactttc accaaaggct tcagtggctc
    20041 tcctggattg gtcgtaatgt aaaattcgat ttggtcaaat ccatcatgat tcaattgtct
    20101 attttcttct tttaagaaac gtacctcaaa ctcagtgttt tccatataca gacttttcaa
    20161 ttcatgcaga atggattttt ccaattcttt tgcgattttt ttacgcgctt catgaagatc
    20221 tgcggcttgt tgctgaagta gaagctcttt ttgctctagt tcctctttca ttttgtccaa
    20281 ttgaccttct gaaaaatcag aagaagctag ctcttcggtg atttcgtcat agtaagccaa
    20341 tattgactca atggattcgc cgtatttacg tttcaactgt cggattaatt ctaaacgttg
    20401 cgtaacttct tctaaacgac tctcatctaa ctccaataaa tcaatctgac gactcatatc
    20461 gccgattgca tcttgcagca aatagtaagc actttggata ttttcataaa ttgcttcata
    20521 ctccacatct agatgggcga tcccttgcag ttctgaaacg gccaagccaa caccatctac
    20581 actgttttgt tcattattcg tcagtgctcc gtaacctgca gctaatccat cagcaatttt
    20641 ttgataattg ctaagttttt ctctttcttc ttttaatttc tcttcttctc cgacttgcaa
    20701 atcagcagca gctatttctt cttgctggaa atggagcata tctattctct gaacatagga
    20761 tttttcattt tgctgcactt ttcgtaccct tttttccagc tcacgataag agatatattc
    20821 ttgctgataa ctctttttct tctgttggaa agctacatct ccaaaacgat ccaataaagc
    20881 aagatgtgat tcaggctgga gcagttcctg atgttcgttt tgtccttgga tatctactaa
    20941 ataactgccg attcttctca gattagctaa tgtgataata tgaccattca ctcgacaaac
    21001 attttttcca gttaaggaca tatcccgtcg aacaatcaga ttgtcctcat ctgtttcgat
    21061 ccctaattca accatcagtt cagagaatcc ttcttgtttt ggtagttcaa acaacccttc
    21121 aagcacacat ttttctgctc cttgacgtat ataatcgctt gaacctcttc ctcctgctaa
    21181 cagacctaaa gcatcgataa taatggattt acccgcacca gtctctccgg tcagcgcggt
    21241 cattccttcg tgaaaagaaa gccgcaattc cggaataatc gcaaagttag taatactgat
    21301 ttcttgtagc atcacattcg ctcctcaact taaaggtaat gcaggaagtc tttcttcaat
    21361 aaaactgcct ggtcttcagt tcgtgtaatc atcaaaacat tatcgtcatc attgataatc
    21421 gaaaataatt ctttcttgta acgtttatca atcaaatttg ctgctgccga agcatttccc
    21481 ggcaatgttt tcaaaataac aaatttttcc atttggtcaa ctgccacgaa agcgtctttt
    21541 aatagttttt ccaatttagt atttacatct tcgtttgttt cagctggtat gctataacgg
    21601 tagccgccat ctgaagccgg taccttgatc agtttcagtt cctttatatc tctggaaatc
    21661 gtagcttgag taacagatat tcctttgctt ttcaggattt caacaaattc ttcctgcttt
    21721 cggatatctt gctcactcaa caatcgagta atcaaacgat gccgatcttt ttttctcatt
    21781 acgtcttcgc tccttagcat attcttctta atcaatgata gcttatttcc ttggtataat
    21841 aaagaagaat ccgcatactt ctcacttgat tttcacagtt acaatttatt tatggtcaaa
    21901 ctgttcgtgt gcatttgaaa ccacttctgc catattaata gctgaattga ttgttccgct
    21961 ttcgggtact ttcctcaagt gagccaagaa ctcgatattt ccttctcccc ccgtgatcgg
    22021 agaataactt aattccagca catcatatcc accttgtgcc gcaaatgaca aaatgtcttc
    22081 taaaactttt tgatgggtct gagggtcacg aacaattccg tttttcccta cagcttctct
    22141 gcctgcttcg aattggggtt tgattaaagc aatcacttct ccctcttctt ttaaaatagc
    22201 atgtaatgga ggcagcatca gttttagtga aataaaagaa acatcgataa cagcaacttc
    22261 tggaattcct aaggtaaagt cttcgggttt tgcataacga aaattcactc tttccattac
    22321 gacgactcta ggatcttgtc ttattttcca agctagctga ttgtagccaa cgtctaaagc
    22381 ataactcatt tttgcgccat tttgcaaagc tgcatcagta aatccgccag tggatgcacc
    22441 aatatctaac aatgtttttc cttcaacaga tagatcaaat gctttgagtg ctttttctaa
    22501 ttttaatccc cctcgagaaa cataaggaag ttttttacct ttgactctta attcactact
    22561 cactggaatt ttttctcccg gtttatcaaa ccgctcgttt ttttcgttgt aaatcaaacc
    22621 agccatcact gaacgtttgg cttgttctct tgtttcaaat aatccttgtt tgaccgctaa
    22681 tacatctacg cgttcttttt ccattctttc cctcatttct ccacatcaaa ttgcttgatc
    22741 atctggcgca tcagcccgcc atcaaaagaa aaaacgtcct cttctaattt gtctatgatc
    22801 ttgttggcgg ataacagctc aatctccaac gcctcacgtg tcttttctag gcctaataaa
    22861 cgcgggtaag tatttttttc catacgttca tctttaccag ctgttttccc taatgttttc
    22921 gttgtaccaa tcacatcgag tagatcatct cttatctgaa aagctaaacc taaatgttcg
    22981 gctaatcgtt gaagtagcaa taagatttct tcaggttgct tcgctaatat gccaccagcc
    23041 agcaaggcat aacgaatcaa atgccctgtc tttctttcat gaataaatgc taattcttct
    23101 aaagaaagct ttttcgattc gccctcgata tctgcagctt gcccagcaac cataccttgt
    23161 gatcccgcac aaacagctaa ttgctgaagc agcagaagct taggagaatt acctaagtgt
    23221 gccatactga ttagctggaa tgctcctgta agcaaaccat ctccggccaa tatagctaat
    23281 gcttcgccga atactttatg attggtaggt tttcctctac gaagatcatc attgtccatc
    23341 gcaggcaaat catcgtggat caaggaatac gtatgcacca tttctaaagc agctgctact
    23401 tgatagacgg gaacatcaat aggctcatca aaggaagcaa cggccgttaa aagtaataag
    23461 ggacgtattc ttttcccgcc tgctttcact gaataactca tcgcatcata gagtcctcta
    23521 tccgttgtat gctcatctaa aaaagacaag atttcttttt ctacagcagg tagatgaata
    23581 gctgaaaagt cagttaatct catctcagct ttcctcgttt ccgtcaaaat caacttcttc
    23641 atttgattct gtcatcattt tcgtcaaggt ctcttctgct ttagctaatg tatcttggca
    23701 atgtttgctt aattccattc ctcgtttaaa cgaatctaga gctgattcta atggaacgtc
    23761 tccttgttct aactgcatga caattttttc taattcttgc agtgactctt caaaagttgg
    23821 attttccata tttttattct cctttgtcta tctcttgtac ttgtccttta acgattccat
    23881 cttgataatg gatcgtcagc aaatcccctt gatgaatctc ggtgacactt tttactactt
    23941 gatcatttaa cgtcgtatag ctgtagcctc ttcccatgat tttcaatgga ctcaacaaat
    24001 caagagattg aacagcttga gcaaaacgct gctgctgttg tttcatatat tgttggattt
    24061 tctcttctaa gcgtttttct agataagcag tttcttggcg agctgcttta actcgataaa
    24121 gcggagccac ttgttccaat tggtggctta atgcgatcgt ttgtttttct ttcgcatgat
    24181 aaatttgctg tgtagcttga tataatcgct gtttcaactg gtctaatttg atggtctgac
    24241 cttcatacaa tctttcaggc tgccgaaata cataggaacg tcgagcacgc tcgaaccgtt
    24301 cttgtttgcg ctggatctgg cgtacaaacg cctgctgcaa tctagcttgc cgttcttgaa
    24361 tgcgcatcaa ctcttctgac aaaacaggaa cagctaattc tgcagctgct gttggagtgg
    24421 cagcccgaac atctgctact aaatcagcga tcgtggtatc tgtttcatgt cctacagatg
    24481 aaatcaccgg cgtattagct tgataaatcg cccgagcaac ccgctcttca ttaaacggcc
    24541 ataaatcctc aatcgaacca cccccacgac cgatgatcat tgtatcgaaa ttgcccatct
    24601 cttctactct ctggatattc ctgacaacat tgtccgctgc ttggtttcct tgtacaactg
    24661 ttgggaacaa aaccaattgg acaattggat atcttctttt caccgtcgtg atgatatcgc
    24721 ggataaccgc tccgcttgga ctggtaagga ccgcgattcg ctttgggaaa cgtggcagca
    24781 tttttttagg cccttgaaaa aggccttcct tcatcagctt ttcttttcgt tcttccaatt
    24841 cttgatataa agcacctatt ccatcaggct ccatgtgctc tacatagatt tgataagaac
    24901 ctcccgcttc atataatgaa atacgcccaa cgacaaggac tttcattcct tcttttggct
    24961 ggaatcgtaa tttttggaat gctcctttga acataatagc agatattttt gcatggtcat
    25021 cttttaagct gaaatattga tgggcattgg cacgtaaacg gaaattcgat atttcgcctg
    25081 ttaaatatac gcgttctaaa taaggatcgg catcaaattt tcttttcaaa tacttcgtta
    25141 atgtagtgac agttaaatat tcttgtgcca tctattcacc tgctccaaaa gcttcggcag
    25201 attttacggt ttgatacatc agcatcgtga tagtcattgg ccctacaccc tttggcacag
    25261 gagtgatcca gcctgcaata ggctccactt catcaaattt cacatcaccg atcagcttac
    25321 cgttagcatc acgattcatc cctacatcta tcactacagc acctggtttt acgaattctt
    25381 ttgtgacaaa atgtccacgt cctattgcta cgactaaaat atctgcttct tgtgccagtt
    25441 cagccagatt ttcggtttta gaatgagcaa cagtaacagt tgcatctgcc atcaagagta
    25501 gctgagccat cggtttaccg acgatattgc ttcgtccgat aactactgct cgtttacccg
    25561 ccggatcgat agaataagct tcaaacattt tcatgatgcc gtatggagta caaggaatct
    25621 tgtccggttt acccgcaagg agtctgccta aattcatcgg atgaaaaccg tctacgtctt
    25681 tttcagggcg gatagctaac aggatctttt cttcatctat atgctttggc aatggtaatt
    25741 ggaccaatat accatgaaaa ctaggatctt gattgtattt ttcaatttct gcaagtagtt
    25801 cgtcttcaga aatcgtctca ggataacgct ctaccaatga atggattccg atttcttttg
    25861 ctacacgttc tttatttctt acatagattt ggctggctgg attttcccct accaataaaa
    25921 cgactaatcc gggatggata ccattatctt tcaatttttg gattttttca gcaatctctg
    25981 cctgcatttt ttcagctaat tcttttccgt taatcaattc tgccatctaa ccactcccag
    26041 atattttttg tgttttcatt ctaccataga attttcccga tttataacag gtttatgatg
    26101 attcatagaa aaaagctagg acttacgccc cagcttctaa ttctttcaat acattggata
    26161 aaacaccgtt gacgaattta cgtgaacggt catcactaaa tgtttttgcc aactcgattg
    26221 cttcatttaa agcaacagcc gctggtacat ctgtcacata agtcatttca aaaatcgcga
    26281 tacgcaagat caccaaatcc atttttgaga tccgattgat gtgccaattg ttttttaaat
    26341 gtttttcaat cactttatcc aattcatctt tttttgcaca gacaccgcca actagcgtat
    26401 caagataaac aggaacaaac tctgattcat cttcgttgat catgtcgcgg tggtctaatt
    26461 cgattgcgtt gaaaatcgca tcttcttttg ttaggtctgc attgaagtct aatggaaata
    26521 aagcctgcaa tgccatttcc cgaatctcgt gtcttgataa ttctttattc attctcttcc
    26581 tcttcgtctt caaaaagttc ttcgattgtt ggttgttcta atttttcagg aacaacagct
    26641 actacatgga tattgacttc agataaatcg atatccgtca tgaaaaggac ttgctggctg
    26701 acccgttcct gcatttccat tgctacttta ggaactgaaa caccatattc tagataacag
    26761 taaagatcta ctttcaagcc atcttcctcg tttgctaagt aaacaccttt tccgtgagct
    26821 gcacgtccta ataattcagt gacattgctg gcaaaagttc cgcgcattcc gtatacgcct
    26881 tctactttag aagctgcaat cccgataatt acttcgatga cttcaggagc gatcacgatc
    26941 tctcctaaat cttggtttgc gtttaaaatc aaattttttt catctgccat ttatttattc
    27001 ctccctttgc ctaagcacga gatacatccg aattctctat gatgcggcat agtgatcacg
    27061 tggtaagaaa actgccgccg atcagctgca aactgctatg aattggagaa aacacccaac
    27121 tttattatct cttacagctt aaaagttctt ctatagtgta tcattaaatc gagtgatttt
    27181 ctataacaaa gtctgtgaac actgattttt acgcttactt cttctattga tgaaactata
    27241 agatgatcaa ttcttttggt gaacgagtca acacacgatt tccatcttct gtgatcaaca
    27301 agtcatcctc aatacgtact ccaccgattc ctggtaagta gatccctggt tcatcagtga
    27361 tcacatttcc tggtacaaat tgcttttctg cacgagaaga aacgtttggt ccttcgtgga
    27421 tctctaaacc gatcccatgt cctgtagaat gtccaaatgc ttccccgtaa ccatgttttg
    27481 aaatataatc tctagcaact gcatctaatt ggacaccagt cattcctggt tttgcagcat
    27541 caataactgc taattgagca tctaagacta tttgataaat ttcttttaat ttttccccag
    27601 gatcaccaat agcgaatgta cgtgtcatat cggaaacgta cccttcgtaa tagcaaccaa
    27661 agtccaatgt gatcaagtca ccttgttcga tgattttttt gcttgctaca ccatgcggca
    27721 tagccgaacg caatccgcta gcaacgatcg tatcaaaaga aacacctgaa gcgccaagag
    27781 aacgcatata aaagtctagt tgatttgcga cttcaatctc agtcatgccc ggttggatca
    27841 ttttcaggat gtgatcatat gccatatccg caatatgaca tgctttttcg ataatagcga
    27901 tttcttcttc atctttcact tctctgagtt cttcgatcat ctgtgaaaca ggtactaatt
    27961 ccgcatcgat gatttcttct aaaaccgaat attctaaaaa tgagactgtt gtttcctcaa
    28021 aagctaatga gtgcagttct tctttgcgta ctaagtttgc tacctcatca aaaataggtc
    28081 cgacattttt aataatttca aacccttgag cctgcgctgc cgcctgctcg gtataacgaa
    28141 aatcagtaat aaaaaatgct ttttccaaag taataacagc taatcctgtc gtacctgtaa
    28201 agttcgttaa gtaacgaaga ttgtacggac tggtaaccaa aaatgaatcc aaattttctt
    28261 cttgcatttt tttgcgcaat ttttctacac gtagcatcat aatgaaactc cctctttctt
    28321 tgcacgcttt tttccattat agcaaacttt tcttcgtaca cgacagttaa ataaaaattt
    28381 ccggaaaact tctttttatt ttaaataaat aaagccaaaa aggagtcaca gtctcgctgc
    28441 aactcctttt agtttactat tttgtaaaaa cttatgatcg ttttccaaag aaaaatgaga
    28501 caactgctac caaaatcact gcgcctaaaa tagatggaat aagtgccata cctgcagctt
    28561 ttggtcccca ttcgccgaaa atcgcttgtc caatacttga accgactagt ccagcaatga
    28621 tattcgttac acagcccatc gaatttcctt tactggtgat cgcacctgca attgctccga
    28681 tcacggctcc aacaatcaat gtccataacc aactcataat ataacctcct tctttgctaa
    28741 tcaattttag catatgggat ttaaagggga caaataattg gttttgctat ttcatatttt
    28801 cttctgcaaa gtaaaaaaat aaagaaaacc tcctcttaga agttttcttt attttttact
    28861 tgcgtttaaa catactacga ataccacgtt tcactacatc aaagaaactc aatgattgta
    28921 ccattctatc ttcatctaca tattctctga tcgcacgaag cactttttta ggttctttgg
    28981 aagaaaatgt gtatgtacca ttttttttcg tttgaatggc atatcgagga atccattttc
    29041 cctttagcat gactgatgct agtacataat ccacttcttc ccatggaatt tggatatatt
    29101 tgcgtctatc tcgcgcatgg taaaactcaa aaccattatt acctatcatg atttgcccgt
    29161 aatctgtcag acccgtaaac gaagtagcat caattaccaa gtctactttt gtattgattg
    29221 attgaaccat tttctccctc cagatatttg tttatgaaca gtatactata taaaaaaagc
    29281 tcgggacaac gcccgagcta tatttttatg gattataaaa gtccgatgac gtgtccaaca
    29341 acaccgacta cgaataaacc aaggatgata acgattggag aaaccttttt cttaagcaac
    29401 cacatacata acaatgttaa tcctaatgca gctaaaccag gaatcaattg gtctaagttg
    29461 tcttgtaatg ttgttacctt ttcaggagaa agggctaagc cattattaac ttgttcaaat
    29521 gcactcttga ttccttcacc acctgaaggc aatttatccc attcgatgta agcaccttta
    29581 tctaatttta cagttgatac aactggtaag aatttgattg atacccatct ttgtacaagg
    29641 gcagctaata cgaacatccc taaaattgaa gcaccttttg ttacgtcttg taacaatccg
    29701 ccagaaaggt catcagtaat tttagaacca gctttgtagc caaattcttg tgtataccac
    29761 atgaatgccc aacgaataat gttccaacct aagaagaaga tgattggtcc aaggatattt
    29821 cctcccatag ctaatgaagc acctagtgca cctagcattg gacgtactgt aaaccagaac
    29881 actgggtcac caacaccagc taaaggaccc atcataccta ctttaacccc ttgaatcgct
    29941 acatcatcaa caggcgcgcc attcgcacgt tcttcttcca gagccaatgt aacccctaaa
    30001 attggagaag caatgtatgg gtgtgtatta aagaattcca agtgacgttt taatgctgca
    30061 gaacgttctt cttttgtttt gtataatttt ttgattgctg gaatcataga aaatgcccag
    30121 ccaccgtttt gcatacgttc gtagttccat gaaccttgaa tgaaggtaga gcgccacgca
    30181 acagctaaac gatctttttt tgataatttg attttttctt ctgccatttt attttcctcc
    30241 ttcaagatta atagtcattc aaaatgtcgc ctaaagggtc accagagttg cttccgcctc
    30301 cattatttga tgaaccaccc attttagaaa ggttcaagta aataagagct aatgcaacac
    30361 ctaaagcacc tagagcaatc aatgttaatt gagaaattgc agctacaacg aaaccaataa
    30421 cgaagaatgg ccatacttct tttgtagcca tcatgttgat taccaaagcg taacctacag
    30481 ctacgaccat tcccccaccg atagccatac catctgttaa ccatgctggc attgattcaa
    30541 ggaatgattg cacagtttgt gctgggataa atagcaacgc tgctgctggg accgcgatac
    30601 gaaccccttg taaacataca gcaatgatgt gccacatttc aaccttacgg atgtttcctt
    30661 cttcagcagc cgcatccatc aaatgaacga ttggaacagc gattgtacgt acgatcattg
    30721 ttaagaacaa ccctgcaaca gccaaaggaa cggcaatcgc gatcgcagaa ggaacacctt
    30781 taacaccttg tccacctaaa actaaaataa ttgctgatgc tactgatgct aatgcagcat
    30841 ctggtgctac agcggcaccg atatttgccc aacctaaagc gatcatctga agcgtaccgc
    30901 caagtacgat accagcttcg aggttaccgg taactaaacc gatcaatgta catgccacta
    30961 gtggttgatg aaattgaaat tcatcaagaa taccttccat accagctaga aaggcaacta
    31021 atacgactaa aattattgaa ataatagaca tgataagcct cctataaatt ttaatattat
    31081 ttattacgct tttgctaatt cagcttttgc ttttttcaaa atatcatcca tgttatcgcg
    31141 tgaatcatta ggtactttac gtacatcgaa tttcacacct ttttgttcaa gcttctcaaa
    31201 cgcttcaacg tcttcttgtc ccatagacaa tactttactt acaacgactt taccgaccga
    31261 atgcgccatt gacccaacat ttaattcttt gatgtctacg ccaccttcaa cagctgtcaa
    31321 tacgtcttca ggattttcaa ataaaagcaa tgctttggta tttccaaaac gagggtcttt
    31381 tgctacttcg atcattttgc taataggaat aacatttgct tttacacctg gaggagcagc
    31441 ttgttcgatt agtctcttac gcagatcatc ctttgatact gcatctgaaa caacgataat
    31501 acggtttggt tgcactgctt ttgtccaagc tgtcgcaact tgtccatgta acagacgtga
    31561 gtcaacacgt gctaatacat atttgatttt tccgtctccg accacagttc cttctggaag
    31621 tgctccttta ggttgtgcgt cttcaactgc tgctttcggt gcttctgccg gttctaattc
    31681 ttccggtttt acttttacac cgtcttttgc tgtttcaatg atgtgtgctg caatttcatg
    31741 agcagattcc attgaaaagc gtgatgcgta ggcttcaatc aacattggta aattcaaacc
    31801 gctgacgatt gcccatttat ctttgtgctc ttcgaataat gtgtttgatt gattgaacgg
    31861 tgttccgccc cataaatcaa ctaagaataa cacttcgtct tgattatcaa aagaagcaac
    31921 cgcttcttcc aatttggctc tcaagtcatc tggaccttca cttggtttca aaatcacggc
    31981 tttgacgttt tcttgttcgc caaagatcat tgaaccggat tgtaagattc cttcagcaaa
    32041 ttgtccatga cttgctagga taatccctac catatttata cctcctattt acttttgttg
    32101 taacaaattc aaaaatatta ataattgcta accattacaa cagaattatt tgaccttttc
    32161 caacaaccat tcttgctaca cctttttgat tttcgctagt agttccatca aaaggatttt
    32221 cctgtcagca ggtacttttc gaatctctaa ctccacttct tgactgttca agtaacgaaa
    32281 ggaatcaata tcttgttgat ttactgaaac aaaattggta atcattgatt tgccttcagt
    32341 aaaactcata ccgccgatat tcaacgactt caactgtaca ccgcagcgga caaccgcttc
    32401 aacatcttgg ggattggtga ataaaagcat cacttttgct cgtgataata atccactatg
    32461 atagatttga atcattttct gcacggttat tacatttgct gtgatccccg gcggtgcagc
    32521 ttcttttagt agaaactttc tcaatttatc ttgagcgact acatcgctga tcacaatgat
    32581 acgttcaaca cctgtttgct tcgaccaaac tgttgcgact tgcccgtgga ttaaacgatc
    32641 atctatacgt gccaatcgaa tatccatctg caaaactcct tcttcctcac tacttcacag
    32701 aaacccctat catttctgtg tgaacaacca cctcctagca cacactacta taagcaaaaa
    32761 gcgtgccaac ttttttacga tacactaatt gaatttaaag gattcttaac caatacacaa
    32821 aaacgataca ctataaaagt gtatcgtttt tgtgtattca tggcacagtg tattcttttt
    32881 tatctaactg attatggact agacgtagca gataatacat ttcttctatc ggcactttta
    32941 cttttatctg atcttccaac agaacaactg aggctgccaa ttggctataa agaggatcat
    33001 tcgttgtatt tgtcaattcg ttttcttcca cagtcaatgg ttcattccga ataatacgtt
    33061 cgatcattcc tgctgtgtgc atcactaggt tgattttgaa tccatattct tcatcgccga
    33121 ttcccgtcgt ctgagagatt tgtgtagaaa attgccacat tggatcgatt aacttactac
    33181 cattaataaa gatgaagtta tcagagataa actcaacaca cgtattttta gctgactctt
    33241 catctaggga gatgaattca gtttcctcca attctgattc catcagcaac tgatccaaaa
    33301 tgacttcgat attttgatca ataaaacgtt ctaaaggaat aaatggggct tggatattca
    33361 aatcagtaat cccagtagta gctatgatct gatattcttt ttggataact ggcagttctt
    33421 ctttcacttc cacaatcgac aaagctagaa cttctaattc tgtttcttga cgcttgttta
    33481 ccgctcgttc gatcaattct ttgattcttt gtgctgtacc ttcgccagat gcgcagatag
    33541 ccaaaacagc ttttttctta tgaataggtt ttgcttcctg aacagattct acagaagata
    33601 tttctgcgta acctcgaaaa tttttcaatg attcatgcaa catatctaga tctgtcccta
    33661 atactgagac ctttcgtaca gtttccaaaa ctagagaagt tgttaccata tcaacagtac
    33721 gtactggaat tccagtgtct ctttgaattt gcgaattaaa cgacgcaagg gaccccatat
    33781 ctactaagag tatgaccccg cttccttcat ctacttcttg tacattgcgt tcaattcggt
    33841 ccaatgctgt tttcggatcc atatccaaag gcatgtctac tgctcgtacg ttctctgcat
    33901 ctaacaactg ttggacaact tggaccatac tgcttgctgt gctgtttccg tgagctgcaa
    33961 cgactacacc gattctgccg cttgcttgat ttgcccgtaa cgagacgagt agaacggtca
    34021 agtaatagtc ttcactttct ggaatctcca cttgaaaata cgtttcgata taactacgca
    34081 cttctttcgc aacagcatat tcatctggaa aatcaaaagc catctcacgg atgttatcat
    34141 tcgtatgccg ttcttctcct aagttgattt ttttcaaaaa agaactgata tgcaagctca
    34201 tagcataaat aaagttttgc tgaaactcat atggtaaaga tcttttgacc attacataaa
    34261 tctgattggt cacttctatg attttcggat ctacaaattc tgctaatttg ctgtctgcat
    34321 taaaagaaaa accatggtct ttatagaaag acttcagatg gatgttgata tctgtagaaa
    34381 tgaaatgatt gattgcttct tgatccaaac catccgactt cagtaaagct gctttatctc
    34441 cgatgatatc atatagatta taaggtaact cataggagtc cgattggatt ttcatgatcg
    34501 tatcatttgg tgctacagtt atctttggct ccaatatttt agaaagctct gccattgcac
    34561 tgcggttgct tgctaattga atcaatccgc tacgaatccc ttctgtcaaa tcatcgattg
    34621 tgatcgagat ttcgggtgat tgcatatgat ttagaaatcc tcgtgcacat actaactgga
    34681 tatttgactt caattgtcct atatttccat aggtcacact gccgatcaat gcttttgcta
    34741 catcttctgt caaagaaatc tttcgctgcg tgcggtttgc ttcgtgggca atcatgattt
    34801 ttactaaatc gactttctca tttgcaggac gtttttcaaa agccggaagt ttgatattga
    34861 ttgggatacg ccgaacaaaa gtttctaata gagaagaacc tgggtcttct gtggtcgcac
    34921 ctacgatccg tacatttgct tcatgggatt tcgttgtttc cccaagacga ctgtaagttc
    34981 catgatccat aaagtaaaag atcatttctt gaccttcagg cggcaaccgg tgaatctcat
    35041 ccaaaaacag cataccgcca tccgcttgat cgataatccc cattttttct tcatctgcgc
    35101 ctgtaaaagc accttttgcg tacccaaata aatgactcat caaaagctca gggttattgg
    35161 cataatcagc acaattgaaa acaatcagtt ccgtttcctc atctattaca tgatgcgctt
    35221 ttgcaaaatg atacatcgcg tgtgcaaaat aagtctttcc tgatccggta ggtcctgtaa
    35281 tcaaacaatt taatcctctt ggcggataca aaatcgcagc ttttgcctgt tccactgaat
    35341 ttttcatgct gccgttagct ccaatgattt ttgcaaagat atccttagta tctatttgga
    35401 gtgtattttt ttcttcagag accggcgttt ctttgtaact gactacatgt tttgtaactg
    35461 gctttacgtg cgtaacgagt gtttgatata tgtaacgtac tggtcgtccg tctgtcttta
    35521 tcagttttcc ttcgcgcact agctgattca gatctttact gctatttgtt cgctgaatcc
    35581 ctaacacttc agcaatttcc tgtgttgtta ctccttgatc atattctgcc tttgttaaat
    35641 gagcggtttt ttctttgaca tacatataaa ttcggtcaat gcgttttgcc aatggtatcg
    35701 ctccttttct atagactatt gtacttaggc ttcaaaacat aatactatgt atttatttct
    35761 gtttcgtcaa ctatttacct gattttgttt tgttgataac acgcggatag atagaaatgt
    35821 tcaatttaaa agaacgaaaa atcccgatac gatttttata atggaaacaa gaaaaagaac
    35881 actgtttacc atataattat ttcactgata gcaaacaata tagcttccat caattgaaaa
    35941 cgatagttat caaaacatcc tgattccaaa ttggcaaaaa aatattaagt gtaaaaagac
    36001 aaagtaaact tcgtagcctc cactacaact tttattttta taaacaaaaa aggctgtgac
    36061 aaaagtcttg tcacaacctc tttaatcata agattcaatt aagcttcttg agctactgga
    36121 taaacagaca cttgtttttt gttacggcct ttacgttcga aacgtactac gccatcaact
    36181 tttgcaaata aagtgtcgtc tccaccgata cccacgttta cacctggata gatttttgta
    36241 ccgcgttgac ggtaaagaat tgatccacct gtaactgttt gtccatcagc acgtttagca
    36301 cctagacgtt ttgattctga gtcacggccg ttagatgtag aaccgccacc ttttttgtgg
    36361 gcaaataatt gtagattcat agataacaac atggtctgca cctcctattt atcattaatt
    36421 gttttaattt ggatatattc tgagttttca gcttctatcg attgtaatcc taataaaaga
    36481 ttttctaata gaatctcagc aatattgttt tgttcttgtg tcattcctga agtgacttcg
    36541 gtataaagat aaccgccttc ttcttcattc atttcaacaa tcggctcaaa accggctaat
    36601 gaggcaatcc cgttgacagt gctgatcgct aacgcagaaa cacccgcaca aacgatatca
    36661 cttccataag gaccagcatc agcatgcccg gtcaaagtga atgaaacgat ctgacctgcg
    36721 tcatttcgct taaatgttcc tttgatcata tataggcctt ctttctcaaa cgctattaag
    36781 cgttgatcgc gtcgataacc acttttgtat atggttgacg atgaccttgt ttacggtgtg
    36841 aatgtttttt aggtttgtat ttgaaagtaa cgactttctt ttgtttgccg tgtttttcaa
    36901 cagtaccttc aacagttgca cctgcaacag ttggagcgcc tacttttgta gattctccgc
    36961 ctactaagat aacttcgtca aaaacaactt tttctcctgc ttcaacgtca agtttttcaa
    37021 catagattgc ttgtcccact tcaactttta cttgtttacc accagtttta ataattgcgt
    37081 acatgctata aagcacctcc ttctgatata cttagactcg ccatccgaag tgacagcata
    37141 tgctgtgctt attacttgtt ctgagcggtt gtagctgtgg agtccacaat tacaacagta
    37201 ctagcttacc agaacagcaa ccgttagtca actaaattta aaatcaaaaa gtaaaacaaa
    37261 aattcaagca atacttctgc ttttctatcc tcacagttct accaaataaa atagtcgcat
    37321 tgtgtttctt ttattcttat aataaaagaa aaaggaggag cataaatgga atacccaaaa
    37381 gaactgatac aagaagcttc tttaagaatg aacggccgtc ctgtagaagg atttatcgct
    37441 gggcaaggac ctcttcatcc taaactgatg ctcgtgggag aagccccagg gaaaacagag
    37501 attgatacgc atgttccttt tagcggtcaa gctggaaaag aattgatgca ggcactttct
    37561 tcaactggct taacacgaga agaggtgtat atcaccagtg ctgtaaggag ccgcccgtat
    37621 cgcgtgactc atcggatcaa taaacggaca caacagtcag aaactgttta tccaaatcgg
    37681 acacctactc gttccgaagt atttgcgcat gcaccaatat tggattacga actgatgaag
    37741 gtccagccaa aattaattgc gacacttgga aacattggtc ttcaacgttt acttggcaaa
    37801 gaatatacgg ttaccaaaaa tcatggtcaa ctctatacag ggcctgtctt acaattaaca
    37861 gaacaaaagg atgcctacga atggtcagaa aaaaactatc gaattcttcc actttttcat
    37921 ccagcagcta ttttctacaa tcgctccctt gtacctgtta tccatcaaga ctggcaagta
    37981 ctagcttcac ttttacagca atattaaaaa gaacagacaa gaccataaaa tcttcagttt
    38041 acagtcttgt ctgttctttt tcattgtttt aatggctaat tgcagtatat ttcgcatgta
    38101 aagctgtcaa cgcatgagca actgtttgat gttgatgcgt gaatgtatgt tcccacaagt
    38161 gagaaagttg tttttctact tctggctgca aggaagtata gataatcgtt tgtcctcttt
    38221 gctctgcttt ttcttgaaat tcatcaaaaa ctgcacgtgc cgtcaaatca atacttggta
    38281 cgcctcgcat ggagaaaatg acaccatcgt gatgatttag ttcttccaat ttatttttga
    38341 gtttttctga agacataaaa aacagtggac cactaatata cactactgtc cagttattca
    38401 gcttttccgt ttctggcagc cccattcttt gccaatcgat cgtttctaat gaaatcgtga
    38461 tagcagcgct tttgacaaca aaaaatacac agccgccgat caccccgatc acgatagcaa
    38521 tacttaaatc aaagataact gtacaaatca tcgtaaggat aaacaataga actgctgacc
    38581 aatatttatt agtaaatagc tccttgatcg tttcccattc attcatacgc caagccgtca
    38641 caatcaaaac acctgcaagt gctggcattg gaaactgtga catcactggc gcaaaaacaa
    38701 gcatggagag cagtaagaca actgcatgta tcataccagc taatctagtt tgtgcaccag
    38761 atttgatggc cacgcttgta cgggcgatgg ccgcagtagc aggaatcccg ccaaagaaag
    38821 gaagcaacag atttccaatt ccttgcgcca ttaattcttg attgctatct aagggcttat
    38881 ttgccattcg gccagccgaa gcaccacata gcaagctttc aatcattcct aataaagcaa
    38941 tgctaatagc tggaactgcc acttgttgca aggcagaaaa gctaaaatct cccatattca
    39001 aacgattact actgatcaat gtttgtggaa tcttacccac agttgctact ggtaaatgga
    39061 acaatagcat aactgctgtt gccaaaatga tagccagcag cgaactaggc attcgtttcg
    39121 cccatttttt tggatagata aacatcccta aaatgactag ccctcccatg atcaaagagg
    39181 ctgtgtttat cggaaatcct atcgtttgat aactacttag tttttcaata acgtttgtac
    39241 cttccgagtg aacaccaaat aaattatcga tctgacctaa tgcaataatg atagcaatac
    39301 ctgaagtaaa ccccgtaatt acgggtgctg ggataaaaga agtcagtgtt cctaatcgga
    39361 aaagtcctgc tgcaagtaaa aatagacccg ctaaaaaagt agcaagcaat acaccttgca
    39421 ttccctttgt tgccgcaata gacatcaaga tagctgccat cgcacctgtc ggacctgata
    39481 tttgataaaa accgcctgat aaaccgccaa taacgatacc ggcaatgata gccgtaatca
    39541 aacctgcggc cgcatcagct ccgcttgata ccccaaaagc caaggctaaa ggcaatgcta
    39601 cagcagctac tgtgatccca gccaaaagat ctttctgaaa tcttgaaaga tcataacctg
    39661 aaaattcttt ttttagtgag gaaacataat ttttgaacac ctatctaact cctttttcat
    39721 gaaattttct aaaataccat tcattagaat atcatatttc atgagataaa gtaatatttt
    39781 tcacaaagtt tcctgttaga taaattctta ttttttctat atttataaga aaatagaaag
    39841 caatgccaac agagctagcc caccttgttt gagtaaaatc caacgatcgc tagtcaaact
    39901 gccatataat gcaacaaaaa aaatagatat taatagcata cttgtgattt ctttagatgc
    39961 ggaagaaaga taagctccat aaagtagtcc cactcctaat agtccattgt acactccttg
    40021 gttcttaaac aatgtttgaa cagattttct gcttaactct tctttactca ttttaaaaac
    40081 cctacttgtc gtgtcagaat cagttctcac cgtttcgata tacatgatat aaaaaaattc
    40141 aattgcaact agagtcacta aaattctaga tagtatcgtc atatcttctt cctcttttct
    40201 gatgagtttt atttttttgc tccttataat tgtactcgat aaggatgttt ttttatttta
    40261 tttgcctttg aaaacaaaca atattggcac aaaaaacaaa gaaaaaccgt tagaaacctc
    40321 agtttctaac ggttttttag cgtcacagga gggattcgaa cccccgaccg tccgcttaga
    40381 aggcggatgc tctatccagc tgagctacta tgacaagaat cagcggaaca tcaataatta
    40441 tagataatta aattgcttct gtcaatacga aatagttatt taattagcaa ataatgaatt
    40501 attttggccc aactactaaa tcaatctttt aaaacgagac attctccttc agctgttcta
    40561 aggttatcaa ttgaaaacag gagctacatt gacttcatgc tactcctgtt tatttagatt
    40621 tcctgtatcc tttaattgac ttacaaatat agaaactttt acatcaagtt cgaattgagc
    40681 cctcgattca atcgattatc tacaggctgc cataatcatg tatcacaact cgattgtttc
    40741 tgattccttt gtcattaaga tcacaccatc acctaatggc aagattgtcg atgtcaaatc
    40801 gggatgatcc atcacaacac gaagaaattc atttaattta cgatggatag ctcgtttgcc
    40861 acggggaatc tcttcatctg ggagcaagat cgttcctcct tggaagatgt catctaccat
    40921 caaaacgcca ccttttctta acaaacgtaa gcattcaggt aaaaattcaa tatattttga
    40981 ttttgcacta tccataaaaa taaagtcata cggaccagac aactccggca agatatctgc
    41041 tgcttgacct tcaagcaaag tcactttatc tgtcagtcct agacgttcat agtttttttt
    41101 agctttctcg atcatgacat cgaaacgatc aattgtcgta acatgagcgt tttctccgat
    41161 tgtcgtggcc atgacacttg atgaaaaacc gatagctgct ccgatctcca agattcgttc
    41221 aggctgaagc tgtccaagta aaaatttcaa aaatacaact gtttcatggg gaataactgg
    41281 tacttctgct acatgagctt cttcttccag tttgcctagc tcacccggta attttttttg
    41341 ctgggtacgc ataaaatcca atagttcttc tttgactaca ggacgatgca tcatttcatt
    41401 gcgcatacta ctcctcctct tcacacatta tacgaacaac tgactaaatc tacaagctgc
    41461 atgagtgttt tctcgtgttt catgttcaga agagtcgttg gatctctgga atacatttat
    41521 gtttttaaca ttcttacaaa aatttctgtg ttcgtctccg cttctttttc aactctacaa
    41581 aggtattcca tgctgtgatt cctgttaggc aaaaagcaat cgtaaagaat aacccctgaa
    41641 tccatgcaaa tgccgttttt gaaaggacta gtccaatata tggaatctga aagatgggac
    41701 taccgataat atttctcttt tttactactc caggctcaat ataagtcttt gtatcagctt
    41761 ttgtataaaa atcttcgtgg ttgttttcaa cggctattac gcgacgcgtc gagatttttt
    41821 tt
//
LOCUS       DAYYHS010000014        41707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000014 DAYYHS010000000
VERSION     DAYYHS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.014"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA6A_000787"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA6A_000787"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8969015.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA6A_000788"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA6A_000788"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8969016.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA6A_000789"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA6A_000789"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8969017.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHA6A_000790"
     CDS             2179..2499
                     /locus_tag="ACHA6A_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8969018.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACHA6A_000791"
     CDS             complement(2611..3468)
                     /locus_tag="ACHA6A_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8969019.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHA6A_000792"
     CDS             complement(3576..4880)
                     /locus_tag="ACHA6A_000792"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8969020.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHA6A_000793"
     CDS             complement(4877..5386)
                     /locus_tag="ACHA6A_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969021.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHA6A_000794"
     CDS             complement(5561..7696)
                     /locus_tag="ACHA6A_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8969022.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHA6A_000795"
     CDS             complement(7779..9434)
                     /locus_tag="ACHA6A_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8969023.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHA6A_000796"
     CDS             complement(9436..9750)
                     /locus_tag="ACHA6A_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8969024.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHA6A_000797"
     CDS             9920..10180
                     /locus_tag="ACHA6A_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8969025.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHA6A_000798"
     CDS             10194..10508
                     /locus_tag="ACHA6A_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8969026.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHA6A_000799"
     CDS             complement(11238..11792)
                     /locus_tag="ACHA6A_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8969027.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHA6A_000800"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHA6A_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHA6A_000801"
     CDS             complement(14153..14869)
                     /locus_tag="ACHA6A_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8969028.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA6A_000802"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA6A_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8969029.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA6A_000803"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA6A_000803"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8969030.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHA6A_000804"
     CDS             complement(18339..19799)
                     /locus_tag="ACHA6A_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969031.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHA6A_000805"
     CDS             complement(19789..21084)
                     /locus_tag="ACHA6A_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8969032.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHA6A_000806"
     CDS             complement(21416..22093)
                     /locus_tag="ACHA6A_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8969033.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHA6A_000807"
     CDS             complement(22123..23214)
                     /locus_tag="ACHA6A_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8969034.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHA6A_000808"
     CDS             complement(23315..23749)
                     /locus_tag="ACHA6A_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8969035.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHA6A_000809"
     CDS             complement(24333..25790)
                     /locus_tag="ACHA6A_000809"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8969036.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHA6A_000810"
     CDS             complement(25787..27682)
                     /locus_tag="ACHA6A_000810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8969037.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHA6A_000811"
     CDS             complement(27740..29515)
                     /locus_tag="ACHA6A_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8969038.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHA6A_000812"
     CDS             complement(29565..31061)
                     /locus_tag="ACHA6A_000812"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8969039.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHA6A_000813"
     CDS             complement(31255..32877)
                     /locus_tag="ACHA6A_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8969040.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHA6A_000814"
     CDS             complement(33205..34518)
                     /locus_tag="ACHA6A_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8969041.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACHA6A_000815"
     CDS             complement(34763..34987)
                     /locus_tag="ACHA6A_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969042.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACHA6A_000816"
     CDS             complement(35007..35429)
                     /locus_tag="ACHA6A_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8969043.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACHA6A_000817"
     CDS             complement(35571..36281)
                     /locus_tag="ACHA6A_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8969044.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACHA6A_000818"
     CDS             complement(36299..36796)
                     /locus_tag="ACHA6A_000818"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8969045.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACHA6A_000819"
     CDS             complement(36833..37285)
                     /locus_tag="ACHA6A_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8969046.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="ACHA6A_000820"
     CDS             complement(37420..38025)
                     /locus_tag="ACHA6A_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8969047.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="ACHA6A_000821"
     CDS             complement(38475..39095)
                     /locus_tag="ACHA6A_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8969048.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="ACHA6A_000822"
     CDS             complement(39097..39966)
                     /locus_tag="ACHA6A_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8969049.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="ACHA6A_000823"
     CDS             complement(39989..40396)
                     /locus_tag="ACHA6A_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8969050.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="ACHA6A_000824"
     CDS             complement(40415..41704)
                     /locus_tag="ACHA6A_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8969051.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7022 g        13943 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgtt
//
LOCUS       DAYYHS010000015        40926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000015 DAYYHS010000000
VERSION     DAYYHS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.015"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1396
                     /locus_tag="ACHA6A_000825"
     CDS             56..1396
                     /locus_tag="ACHA6A_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8969052.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1614..2057
                     /locus_tag="ACHA6A_000826"
     CDS             1614..2057
                     /locus_tag="ACHA6A_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8969053.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2561..3979)
                     /gene="nhaC"
                     /locus_tag="ACHA6A_000827"
     CDS             complement(2561..3979)
                     /gene="nhaC"
                     /locus_tag="ACHA6A_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8969054.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4382..5605)
                     /locus_tag="ACHA6A_000828"
     CDS             complement(4382..5605)
                     /locus_tag="ACHA6A_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8969055.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5636..6475)
                     /locus_tag="ACHA6A_000829"
     CDS             complement(5636..6475)
                     /locus_tag="ACHA6A_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8969056.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6492..7172)
                     /locus_tag="ACHA6A_000830"
     CDS             complement(6492..7172)
                     /locus_tag="ACHA6A_000830"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8969057.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7174..8247)
                     /locus_tag="ACHA6A_000831"
     CDS             complement(7174..8247)
                     /locus_tag="ACHA6A_000831"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8969058.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8303..8541)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8584..8817)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8894..9565)
                     /locus_tag="ACHA6A_000832"
     CDS             complement(8894..9565)
                     /locus_tag="ACHA6A_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8969059.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9652..12300)
                     /gene="mgtA"
                     /locus_tag="ACHA6A_000833"
     CDS             complement(9652..12300)
                     /gene="mgtA"
                     /locus_tag="ACHA6A_000833"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8969060.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12642..12816)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12989..13426
                     /gene="ndk"
                     /locus_tag="ACHA6A_000834"
     CDS             12989..13426
                     /gene="ndk"
                     /locus_tag="ACHA6A_000834"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8969061.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13448..14314)
                     /locus_tag="ACHA6A_000835"
     CDS             complement(13448..14314)
                     /locus_tag="ACHA6A_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8969062.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14474..16567)
                     /locus_tag="ACHA6A_000836"
     CDS             complement(14474..16567)
                     /locus_tag="ACHA6A_000836"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8969063.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17316..17939)
                     /locus_tag="ACHA6A_000837"
     CDS             complement(17316..17939)
                     /locus_tag="ACHA6A_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969064.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18098..19495)
                     /locus_tag="ACHA6A_000838"
     CDS             complement(18098..19495)
                     /locus_tag="ACHA6A_000838"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8969065.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19527..20498)
                     /locus_tag="ACHA6A_000839"
     CDS             complement(19527..20498)
                     /locus_tag="ACHA6A_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8969066.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20872..21297
                     /locus_tag="ACHA6A_000840"
     CDS             20872..21297
                     /locus_tag="ACHA6A_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969067.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21785..22486)
                     /locus_tag="ACHA6A_000841"
     CDS             complement(21785..22486)
                     /locus_tag="ACHA6A_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8969068.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22502..22942)
                     /locus_tag="ACHA6A_000842"
     CDS             complement(22502..22942)
                     /locus_tag="ACHA6A_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8969069.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22942..23517)
                     /locus_tag="ACHA6A_000843"
     CDS             complement(22942..23517)
                     /locus_tag="ACHA6A_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8969070.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            23758..23967
                     /locus_tag="ACHA6A_000844"
     CDS             23758..23967
                     /locus_tag="ACHA6A_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8969071.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(24365..24877)
                     /locus_tag="ACHA6A_000845"
                     /pseudo
     CDS             complement(24365..24877)
                     /locus_tag="ACHA6A_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(25003..25704)
                     /locus_tag="ACHA6A_000846"
     CDS             complement(25003..25704)
                     /locus_tag="ACHA6A_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8969072.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25812..27255)
                     /locus_tag="ACHA6A_000847"
                     /pseudo
     CDS             complement(25812..27255)
                     /locus_tag="ACHA6A_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(27269..29134)
                     /locus_tag="ACHA6A_000848"
     CDS             complement(27269..29134)
                     /locus_tag="ACHA6A_000848"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8969073.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29255..30105)
                     /gene="licT"
                     /locus_tag="ACHA6A_000849"
                     /pseudo
     CDS             complement(29255..30105)
                     /gene="licT"
                     /locus_tag="ACHA6A_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            complement(30415..31128)
                     /locus_tag="ACHA6A_000850"
     CDS             complement(30415..31128)
                     /locus_tag="ACHA6A_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8969074.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31196..31522)
                     /locus_tag="ACHA6A_000851"
     CDS             complement(31196..31522)
                     /locus_tag="ACHA6A_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8969075.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31645..31872)
                     /locus_tag="ACHA6A_000852"
     CDS             complement(31645..31872)
                     /locus_tag="ACHA6A_000852"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8969076.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(32194..33447)
                     /locus_tag="ACHA6A_000853"
     CDS             complement(32194..33447)
                     /locus_tag="ACHA6A_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8969077.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            33508..34248
                     /locus_tag="ACHA6A_000854"
     CDS             33508..34248
                     /locus_tag="ACHA6A_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8969078.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(34334..35092)
                     /locus_tag="ACHA6A_000855"
     CDS             complement(34334..35092)
                     /locus_tag="ACHA6A_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8969079.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(35089..35553)
                     /locus_tag="ACHA6A_000856"
                     /pseudo
     CDS             complement(35089..35553)
                     /locus_tag="ACHA6A_000856"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(35572..36069)
                     /locus_tag="ACHA6A_000857"
     CDS             complement(35572..36069)
                     /locus_tag="ACHA6A_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8969080.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(36137..39304)
                     /locus_tag="ACHA6A_000858"
     CDS             complement(36137..39304)
                     /locus_tag="ACHA6A_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8969081.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(39528..40412)
                     /locus_tag="ACHA6A_000859"
     CDS             complement(39528..40412)
                     /locus_tag="ACHA6A_000859"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8969082.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(40507..>40926)
                     /locus_tag="ACHA6A_000860"
     CDS             complement(40507..>40926)
                     /locus_tag="ACHA6A_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969083.1"
                     /translation="FLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVLVLGTL
                     IIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPP
                     KIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        11705 a         8614 c         6972 g        13635 t
ORIGIN      
        1 gctatttatt cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga
       61 agataaaaaa ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg
      121 agcaatcggt gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc
      181 tgtattgatt gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg
      241 aaaaatcgtt ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta
      301 tctaggtgct cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa
      361 tatcatcgct gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc
      421 agcctgggta tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt
      481 acggttattc gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt
      541 actgatcatt tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc
      601 tacccttcag caattgactg accatggtgg gtttactcct catggtatga agggaatcat
      661 cagttctttg cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt
      721 aagcgaagca gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag
      781 aatcatttcc ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac
      841 tttagcaggc acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt
      901 tgcagcggat atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg
      961 tgtgtatgca tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag
     1021 aaaactagct gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag
     1081 tgttctttat cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct
     1141 ggcaggttcc ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt
     1201 tgtactggca ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc
     1261 tgtcttagga ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt
     1321 gactggcctg ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt
     1381 attgacgaat gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc
     1441 tattgaagaa aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt
     1501 ctcatgacct cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct
     1561 ccatgctcat gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa
     1621 agtattggaa gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt
     1681 acttgaatgt ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt
     1741 tgatgatggg tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa
     1801 acaaaaagat aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt
     1861 ttatggtacg aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc
     1921 atcattctat cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa
     1981 aattagcgga agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga
     2041 tcaaagaaat ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta
     2101 tcagcttcaa gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa
     2161 aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg
     2221 tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta
     2281 aaatcagcgt tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt
     2341 gccatttttt gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt
     2401 gaaaaagtcg cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt
     2461 tgtataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg
     2521 gattgtgaca aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg
     2581 tcttgcttac tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg
     2641 cactgaccat cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga
     2701 gtgtaggaat accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt
     2761 agttgattac tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg
     2821 ctttttcata agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac
     2881 caataaaaat atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt
     2941 tcgtcatctt tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca
     3001 atccacctaa tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc
     3061 ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt
     3121 cgatgacccc agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta
     3181 ctgctacagc gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca
     3241 gtaacattaa aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa
     3301 catcagtcac ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca
     3361 tgaataaaaa gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt
     3421 gtttgaacaa atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag
     3481 acatcttatc tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta
     3541 agcctagtgt tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg
     3601 cactccctac agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact
     3661 ggacactgat caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc
     3721 atacagcaat caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga
     3781 tgccattgat gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca
     3841 acatgaccat tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa
     3901 cgccaatcgc aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg
     3961 gtgttccttt ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa
     4021 gttaagatat cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa
     4081 aagacctgtt ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat
     4141 ctgtttttca ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt
     4201 aggcttgatc ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc
     4261 gtgaaaagtt gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa
     4321 aatatttttt catttttctg aaaattattt ttataacttt cacacacaag tttatttttt
     4381 actagaaagt agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga
     4441 ttctttctaa tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg
     4501 ctgcaatcgc aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg
     4561 tgtgtccggc aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt
     4621 ggctgacatt tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt
     4681 catctggaac acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa
     4741 cgatgacatc atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta
     4801 tcgcagcacc tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat
     4861 ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc
     4921 ctccgtctgt aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt
     4981 tgattccatt aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg
     5041 ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt
     5101 ctgtcgtttt cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa
     5161 aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg
     5221 gggtgccata tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg
     5281 cggctaatac ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta
     5341 aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg
     5401 catcaaaggc tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta
     5461 attgtttaat caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt
     5521 ggatatctag agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt
     5581 ggtttgttga gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt
     5641 ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt
     5701 tgactgtgtc ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat
     5761 cttcttttct tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta
     5821 agaagatggc atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca
     5881 ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt
     5941 ctagattttt tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat
     6001 ccactttgat caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat
     6061 cattagggat tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag
     6121 aataaattcc cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt
     6181 ctttattgta attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat
     6241 ctgctaaagc cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt
     6301 tctccttttt caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc
     6361 ctagttttac gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg
     6421 ccaaccaaat aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt
     6481 ctcctccact ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa
     6541 aaaactaaga ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta
     6601 taccctctcg atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta
     6661 gtcagtccga tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct
     6721 tctaacaaat acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct
     6781 tcgatcaccc caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga
     6841 acaactccaa aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag
     6901 aaacgagtag tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata
     6961 ttgatcagct tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt
     7021 gtcactacta acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa
     7081 aaagacatat atagtgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca
     7141 ttgggcaaat atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat
     7201 acgactatct aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata
     7261 atccaatgct tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa
     7321 cggcgtttct tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt
     7381 gctgtacaga tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac
     7441 cagccattta ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc
     7501 ttggtcaaga tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct
     7561 gaagatctgg atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt
     7621 gttgcagatt tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa
     7681 ctggcgattc aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc
     7741 actggttgct tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc
     7801 tactcgttgt ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa
     7861 accgacaagt tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga
     7921 atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg
     7981 ctgaaagatc ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact
     8041 cagattcgtc tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac
     8101 acgaactaac gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt
     8161 ttcaatcgtt aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca
     8221 tacagaaaca tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc
     8281 aagaaaatga aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc
     8341 aagctgtgtt cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc
     8401 gcagccgtca tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat
     8461 agtcgttcct tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac
     8521 ttaccatttc aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt
     8581 cagtaaaagg acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc
     8641 caacatctca aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc
     8701 tacttatcga caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca
     8761 ccagccagag cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat
     8821 tttgttgttg ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt
     8881 attttttcta tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat
     8941 cttttccttc cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa
     9001 aactggaatc aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta
     9061 ctaaagaatc ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg
     9121 tcagataagg aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt
     9181 ccaagtaatc agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg
     9241 tcgtagcaat gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat
     9301 atggttttaa gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg
     9361 ctcggacttc ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt
     9421 gtttccagtt ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt
     9481 gctgaaatcc ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat
     9541 ctaaatcaaa tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag
     9601 tttagcacga ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag
     9661 ttcttggaat cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat
     9721 gactgtagca aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc
     9781 accaaaactt gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa
     9841 catggcaaat gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag
     9901 cactaaggtt tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa
     9961 aatcactcgc tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat
    10021 aacaaaatac atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta
    10081 aaccatgaat tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt
    10141 atccatccga tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa
    10201 aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa
    10261 catattgcca aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt
    10321 ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac
    10381 gtctgcagat tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac
    10441 tttcattgca ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt
    10501 acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg
    10561 gtcttcagct acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac
    10621 caattcattg atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc
    10681 cccagtcaga acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt
    10741 tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc
    10801 tttgactgaa aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg
    10861 taagccatct tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt
    10921 taatggtgta acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat
    10981 cgcgcctttt gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg
    11041 acgacgttca aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc
    11101 taaagtttct gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg
    11161 atagtagcta tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc
    11221 catatgatat tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa
    11281 cacatcgatt gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt
    11341 ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg
    11401 tgttagacca actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt
    11461 aagcccattg atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat
    11521 ggatgtttta tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc
    11581 tgctacatga ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc
    11641 actgatgaca ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc
    11701 gtcttcacat actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg
    11761 ggaaatgaat aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc
    11821 tgaaagccga acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc
    11881 attacgtttt acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt
    11941 ggaacgtacg gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat
    12001 ggctacaccg aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga
    12061 aataattcct aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc
    12121 tacaatgaat ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga
    12181 acgttcgatt tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc
    12241 ttgtttcgtt tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat
    12301 ctcaatctct cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat
    12361 tcatcggatc gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg
    12421 cctttcctct agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta
    12481 ctaagtaatt ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga
    12541 ccacctcctt tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa
    12601 atactcagcg aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga
    12661 gttttgacac tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc
    12721 ttatctcgac aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct
    12781 atgtttgtat aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca
    12841 actaaaaaag aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac
    12901 acgttatatt cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata
    12961 gaaatatagt aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga
    13021 cggcgtgaga cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc
    13081 cattgctgaa atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca
    13141 cttgactgag cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt
    13201 ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac
    13261 aaaagctgcc gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga
    13321 aaacatcatt cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt
    13381 cccgccattc gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac
    13441 tgctggtcta gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt
    13501 gattctttcc taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt
    13561 cttcccctaa agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga
    13621 tcactgtact tctttcttta tgtgataagc tccgtttcat atcttcattc acactacgtg
    13681 cctttttcaa ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga
    13741 gatactgctc atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat
    13801 aattgttctc aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt
    13861 caaattgacc atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac
    13921 gtaaaaattc aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa
    13981 ccaaataata cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat
    14041 aaggatcaaa gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta
    14101 ttggttcatc cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct
    14161 ctaaaaatga tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga
    14221 ctagagaagt cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga
    14281 ctcgaagttt cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt
    14341 tttccacatt caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa
    14401 tgtttttttc ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg
    14461 ttggtttgat tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat
    14521 ataagttggt tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt
    14581 tttgcttttc gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat
    14641 caatcgtctc aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat
    14701 ctgaaagact taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc
    14761 ctgctgcttt cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga
    14821 cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag
    14881 aagataataa gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta
    14941 gtaatgtcgg caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat
    15001 aaccaggcat atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc
    15061 atgtatctgg atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc
    15121 caaaatgatc cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat
    15181 acgtaaaaaa ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta
    15241 ctgtgctatt gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag
    15301 gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat
    15361 attgggcaga ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg
    15421 attcatcaaa ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat
    15481 ctctattcca aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt
    15541 gtccaaggat cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg
    15601 agccttgagg taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc
    15661 cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac
    15721 tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga
    15781 aactttctaa atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat
    15841 ttggctctgc ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg
    15901 catgcgcgcg aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat
    15961 cgattcctag atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac
    16021 ggttggcttc gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag
    16081 cgatagccat cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc
    16141 aaaccagtag cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca
    16201 tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt
    16261 tgactgtaat gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg
    16321 tatttaaaat atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca
    16381 aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact
    16441 ggatcgtacc ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc
    16501 agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt
    16561 gtgtcacgta tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga
    16621 aaatttccta aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt
    16681 ccacttttca cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa
    16741 gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca
    16801 aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct
    16861 ttttcacaac ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa
    16921 tatttataaa acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg
    16981 gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat
    17041 gtcaggacgc taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt
    17101 aataagccca accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct
    17161 cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccttcg
    17221 gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac
    17281 tcaggtcaaa acgctgattt cacaacctct gtttttcatt gtccatagga ttgatattgt
    17341 ccttgtgatg actcattcgt aataattgag gcttgatttt cttcacttcc cgttatcgtt
    17401 tgtccactgc cagaatctga aggacttgtt cctgtcgact cgtttttttg agaagagaca
    17461 gttgttttct tgacttgttc atttgtcttt ttccattccg ctaaacgatc ggcgaccact
    17521 tttttgtcta ctttttcaga ataagcggtg atcacttttc catttttcaa acgggctact
    17581 ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa ttgatattgt
    17641 ctgagagaaa tcgcactaag tacgtcatcg atattaataa attgattttc ttcttttcct
    17701 tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt atactccgcc
    17761 caccaataag ttgagaattc ttttttgaaa ttatctactg attctttgct tgctacgatt
    17821 ttttctgttt ctttctgagc aagatctgct gtcatttttc ctgaaaatgc tgcggataaa
    17881 ataagcagta agaaaaaacc gaataaccaa aagagaagaa acggtctttt tttcctcatg
    17941 ctttcctcca ctcctattta attttgacta ttctattata tacaaaaaat gaaatagaaa
    18001 gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa atggtaagaa
    18061 tggagaattt cacgcatttg ctctcaacta ttctttctta agcctcaaat ccgtttttat
    18121 ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt tctttttcca tcttgtgcta
    18181 agtctactga aataaagcca tatcgattct tatatgcatt ggtccacgac cagttatcca
    18241 tacatgtcca catatgatag cctaaacaat tggcaccttc ttgaatggct tgatggacat
    18301 atttcagatg agattggaca aattcgatcc gataatcatc gtggatcatg ccatcttctt
    18361 tgataaatcg tgcttcgttt tctacaccca taccgttttc agaaataaag cagtcgatat
    18421 ttccataatg atctcgtaca ttgatcaaaa tatcatagat tccttgttca tagatctccc
    18481 atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaaa taatcctcag
    18541 gcattggacc gtttttagta tcgattggtg tttcttttgc ttttacccgg cgtggctggt
    18601 aataattgat acctaaaaga tctactgtat tttctttgat aaccgtcaaa tcctctgcat
    18661 ggatcacagg catgagatca agttctttta ctatctcaat caattcactt ggaaactcgc
    18721 cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt tgcgctgctt
    18781 taacatcttc agggtttgat tcgtctcttg gatagcttgg tgtcagattc aagatgatac
    18841 caataacacc accttgctcc atttcatggt aagatttgat ggctaacgct gaagccaacg
    18901 cttcatgata gccaacttgg atcgcttttt tcatatctga ctcttctgga taatgccatc
    18961 gatataaata acctgcttcg actggaacga ttggttcatt atgagtaaac cattttttta
    19021 ctttcgaacc aaacaattca aaacaacttt tggcatattt ttcataggct tcaaccgttt
    19081 ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa tgatacagat
    19141 tcacaaaggg ttcgataccg ttttcaatca actcatcaaa ataacgatga tagaaacgaa
    19201 cggcttcttc gttgattgtt ccagattcat caggtatcaa acggctccat tggatagatg
    19261 ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga tacttcgtat
    19321 aaacttgcga ggttttttca ggtcctactt gatcaaagaa cttttgcggt tcttttttgt
    19381 accaataatc aaaaatgttc tctccttttc catcgcctga aatccttcct tctgtttgtg
    19441 ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt ttcatcaaag
    19501 cacctctttt tcttttttag ttcgttttag ttgattccat gtatgatatt ttccatcaca
    19561 atcacttttt cattttcttc ctcttctttt ttgattcgtt ttaacagctg ttctgctgct
    19621 cgttccccga ttttatctgt tggtggcgtg atcgtgattt tcggaaggat actcagatcg
    19681 tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct
    19741 tgattgaaac attcgatggc accaatcgac atattgtaat tgctgacaaa aatagcagtt
    19801 gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct
    19861 gtaaagacct ctaattggat cgatggatca ttttggaccg cttctttggc tcctgctaaa
    19921 cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa atccgatttt tttatgtcct
    19981 tgttcaccat gatgagcgat cacttttgct acactttctc gatcattcac taatattgta
    20041 tcaatacctg ctggtccttc tggacaattc aatgcgataa ctggaatact catttttgtg
    20101 agcatttcaa atccagacca ctcttcttcg gataagaaga caatcagccc atcgatcaca
    20161 tgcttcatca ggtattcaat tttttgttca attttttctg gctgatcggc aaacccgctt
    20221 aataataagc tatacccatt ttcttcagct actctttcga tccctgagac aacttccgac
    20281 ccaaaacggc tggaaatccc gtttatcaaa agtccaatag agaagcttcg attattcttc
    20341 aaccctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgctattttt
    20401 ttcatgttct tttccttcag ttgctgacca ttcaagtaac gggaaactgt tcccactgaa
    20461 acaccagcca tcttagccac atcacggatc gatgccatac attctcactc ctgccagtta
    20521 catttgattt ctctttcttg ttgttcgatt atgatgaccg cattctttgc atagtgttgc
    20581 ttgtcatcaa tatccatata actaaagtct tcagtagagc aacttcctct cactattaat
    20641 tatacttaaa caagtatttg aaccggttca tttttaatat agcatttttt taaaagattg
    20701 caacactttt ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt aaaattttat
    20761 tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc cgtttgaagt
    20821 tttacattcc aatggtataa tttctttatc ctatcaaatg ggggaagtat tttgaagaaa
    20881 ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg tgatttgttt
    20941 ggcggctctg accaatcctc ggacgaaaca accgattctt ctgtcttaaa agcggaaaaa
    21001 gcagatacca ccaacagtgt atatacgcta aaagtacgta aagatatgga tttatccgcg
    21061 ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca gacacaagga
    21121 actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa gatcactacc
    21181 aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaaacaac agtcatattt
    21241 acaaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag tctatagtgc
    21301 cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa gaagcaagat
    21361 gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat gtaataacaa
    21421 caaaaatatc catacagaat ttaatgacca aggaagcttg agcatatgcg acaactgtca
    21481 caaactagtc aaagattcat ttaaaacatt tgaatcttat cctgttcaaa attatgtaaa
    21541 aagtagaaga agataaacaa atccaagcaa aactcactat ggctgatttt tatgaacttc
    21601 aaactcagct tcttcctttg atcaagcttc atcagtaaaa caaacagtgg atcatttcag
    21661 tttacaaatc aaagagtaaa gatttattta tttcaaatat ttgaatataa aataaccaaa
    21721 cgatagaaag gcttattcta catcacgtag taatttttct ttcccgtcgt ttggtttttt
    21781 atttttagcc taattcttga ccttttgcat gctggttatc cgtaaaatat tttccagcag
    21841 gctttccttc caattcagct aatttttctt ggatctgacg gatcaccttt tttgcatctt
    21901 ctggattttc tacaaaatcg aatttatccc catccacttg tattttagga catacatcaa
    21961 aatcttcata ccattgatta tagcgggtgt tcaacgtttc ataataggta tataacgtct
    22021 catcgctttc tagctgttca tagtcacgcc cacgtttttt gatacgttct agcatcgtgt
    22081 ccaaagaaac tttgatatgg accatcaaat caggccgttt ttttggagca acatcatcta
    22141 actcgtttag catcgtatct aataagctat cgtactgctg tacttcgata tccgttaccc
    22201 gacctaaatc agcgtttaga tggaacaaca ggctatcttc atagatggat cggtctagca
    22261 cattgtcatc gttcactaaa gcatttttca ttgctaaaaa tcgcttgttc aaaaagaaga
    22321 tttgcaataa aaaagcgtac tgctcaggat ttgaataaaa gagcggcaat acttcgttat
    22381 cttcgacatt ttcataaaat ggtttagaat caatctcttt tgcaagcata tccgtcaaac
    22441 tgctcttacc tgcaccaatc gttcctgaaa tcaaaatcac tgacatacaa cccttcctct
    22501 tctataagta ctcgcctacg taagtttcaa caggcaatgt ttcaaaatca tattcacgta
    22561 cagcttggtc tgattttaaa tacgtatgga gactttgtgt aatcatcaag ttcgtaggta
    22621 ccgtctcttc ttgtacaatg atcatcggtt ttttcaacat agttgccaca cctaattcgt
    22681 aagccgttcc tggatcgatt gtttgatgct caaagtcaag gatagcaaca acaaattctg
    22741 catttgtcag ttcttccata tctttttcgt atacttcctg cgcccatcca gcgctgaata
    22801 gttcataatt gctttcttgg tgatgacgtg gagagtagaa acttgtgact gttttgtttt
    22861 cctccaacgc tttttcgata cgacttactc gatcgatttg ctcctctgaa aaaaatggtc
    22921 ctgctaaata tatttgactc attattttgc ctccttcaat aataactgat ttttaataga
    22981 tactgctcgt aattcattga ccagttccaa tggattgttg gaaaagatta cttgatccaa
    23041 ccatttttta tccataaaat cctcttttgc aaaagtttct aataattgtt gcaagccgct
    23101 gtaataaccg ccaatattca atattccgat tggcttgtcg ggcgtcagac cgattgctat
    23161 ccaagaaaga agttgtgaga attcctccaa tgtacctaga cctccaggga aaacgaggac
    23221 agcatctccc ttttcaaata ataatacctt tctttcatcg atcgtttctg taggaataaa
    23281 tgtagtaaca tcttttccag gcaattcagt ttcaaaaagc ccattcggat agatacctgt
    23341 gacttttccg ttattttcca gtactgtttt actaatcact cccataatcc cggagtatga
    23401 accaccgtaa accagttcga tttcttcttg tgccatgtat tttcctaacg tttgcgcaat
    23461 aaatttgtaa ctttctttat taccaaaacg ggaaccgcaa aaaacagtaa gtttcataaa
    23521 aaaacactcc ctaactcaaa agtctatctt cattctacac gtgttttttt aaaataaaag
    23581 actagatttt gtagtttttt agtggtatat aggatagacg ccactatata tagtatttag
    23641 aaaaaaatca tttttcagcc attttataga taaaacaaaa ataaataacg tgttttcatc
    23701 gatatgaaat gacttactta tagaagatac tgggatttcg tcctatatgc caataacgtg
    23761 tattatttta aacaacaaat acttgtttct tttattaacg aaaaaatcat tttgttctcg
    23821 tgttatgttt cactgataaa tgatgtttct ctaaataaaa aagccgacga actaaacgat
    23881 caaaatttga tcatttttgt tcatcagctt ctcctaaaaa tctttttgtt aacgataaat
    23941 agctggattt gtaaaaatat cacttgaatc gtcactgctg gaagaaaatt cagtgataac
    24001 cgcacctgtt gcgcctgcct ggaactagtg tttgatattg ggctcgaacg tgtactgatc
    24061 tctagcatga agaataattt ctttttttac tgtataataa ctttctttcc tttttggaat
    24121 agtacaggaa agtttttttg tttcctttcc ttcgacataa ggtataccag accctttcga
    24181 caacgaaagg ttctcatttt tccttcatcg tcatttcaag gagggtgccg atgctctgga
    24241 cagtttcgat cgctcctaaa ccaaaatctg tatattcgat tctttctaac tctgattctt
    24301 tcataccatt cctgtctcaa ggtacatcta ttgtactttt ttcttgtaat ccattctgcc
    24361 tctcctaact aattgtcaaa tcatcccaag atatgtcttc ttcctctttt tcttcttgga
    24421 tgatttctgc attcggatct actggttttt tcaataataa aatggttatt cctgtgacaa
    24481 aagaaccagc taacaatgca ccaatgaatg cccatggtct ggtcatcgta gcgactgcaa
    24541 acattcccga tgccggaata cggctatctg cgccccaaaa catgcttaat ccgccaccta
    24601 ctgtcccacc gactcccgtt gctatgacat tgcgtgccaa gtcgttcaat acgataggga
    24661 aacatccctc tgtgatctga cagatcccca taggaaaagc ggtcttcaat gtatcgattt
    24721 cttgacgatt gagtatattt ttacgaatga ttttccctaa gaaataagcg atcgtcaagc
    24781 cgaatggggt taccatcgaa gctaaaatca atacagtgat tggttcatag attccttctg
    24841 aaaatcgtgc cacaccccct tatccagaat aaatggtaat ccagaaacag ccgtgtatct
    24901 gcggtaataa gcctaaccaa acgaaaaata tgagaagcta tttttgtttg gctgttctta
    24961 ttacttgatg cagggcagct ttttcacaac ctcttttttt atttatgttt cataatcaat
    25021 atggtaagga tgcagctccg tatggtagac cgcctccgaa tattcaatca tttccccaat
    25081 ttgcgtagac gatttccgta tccgtttgat gccgatctgt gcgtctgttt tcagaattcc
    25141 ttgttcttct tctgttaaaa atactgccga gaaatcatca gaaaatgagt caatagtata
    25201 tccttttttc gtcagcaaac gatacaaaga ttctttgcca aattcttctc tgctgaattg
    25261 catcatttct tgtggaagat aatgaacaaa ataaatataa ggctgattgt tcaaataata
    25321 taagcgctta aaacaaacag cttgctcacc gaaaaaggaa aacaaaggat cttccttctt
    25381 tatatggacc aattgcaaat ccagcgtttc tttccggact tcatatcggg agttctccaa
    25441 gatttgcgta aagcttgccg ctttggataa tcgattgtaa ggacgattgc ttagaaccgt
    25501 cgtcccacga ccacttttct ttgcgacata ttcatctgat gccagcagtt cgattgcctt
    25561 tcggacagtg attttgctga cttgaaacag ttcttctaat tcactttctg tcggcaacat
    25621 cgaaccgact ggatattttc ctgagaaaat atcttcttta attttatctg cgacttccaa
    25681 gtatagtaca cgtgtccttt tcataacgtc accctttgct ttactattac taaaagaata
    25741 gcacataaaa aataaaaaaa gaacacaaat cacatgtgag aagtgtgttc tttttcattc
    25801 agctgcttta attacaagtt tacgccattt gaggcaatta cttctttata ccaatcaaaa
    25861 gattttttct ttgatcgatt cagtgtccca tttccttcat cgtctagatc cacatagata
    25921 aagccgtaac gtttactcat ttctcctgtt gatgcactga caagatcgat acatccccaa
    25981 ggcgtatagc ccatcaaatc tacaccgtct gctactgcat tagccatcgc ttcgatatga
    26041 cgacgtaaat aatcgatccg ataatcatca ataatcgtgc catcctcttc catatgatca
    26101 atggctccaa gaccgttttc aacaatgaat aatggtttct ggtaacgatt gtataattca
    26161 ttcaatgcga ttctcagccc ttctggatcg atctgccagc cccattcact tgcctcaaga
    26221 aacggatttt ttactcctcc cagcaagttc ccttctgaag ttgctgcttg tggatcagtc
    26281 tcattgattg ccgtagacat ataataacta aatccgatgt agtccactgg atattctttt
    26341 agcaactgga agtctttttc cgtttgctct aatttttccg gatcgactcc atatttttta
    26401 tgggtacgcg ctgtgtatgc agggtattct ccacgtactt ggacatccgt gcagaaaaaa
    26461 ttgaattctt ggttttggat cattgttgct gcctgattga ctggattggc atcgatgctg
    26521 taagttgttg catagataat catgcagccg atttggatat ccgaacgcaa ttcgtggccg
    26581 atttttacgg ctttgctact tgcaacgaat tgattatgcc atgcttggaa aatattttgg
    26641 tactctcctg ctccattgct ttttactaat ccttgactta aagctggaaa gtgaaaggcc
    26701 gaattgattt cattgaaagt catccaatat ttgactttac tgctgtatct ctctaatact
    26761 gtctgagcaa atcgttcata gaagtcgatc agttttcgat ttttccagcc accgtattct
    26821 tttgccaaat gaagaggcat ttcgtaatga gaaatcgtga tcacaggttc aatatcatat
    26881 tttaagcact catcaatcag ctgatcataa aattctaatc ccgcttcgtt aggagtacct
    26941 tcgtctccat ttggaaaaat tcgtgtccag gcaatcgaaa aacgataaca tttgaatccc
    27001 atttccgcaa acaaggcaag atcttctttg aaacgatcgt aatgatcgat tcctcgatga
    27061 ttcgggtaaa tatatttttc ttgatcaatc gtccagtcaa attcttcact gccgataatc
    27121 gcaaagcgct gcttacctcc aggcatagca tctgcaacgg atagtccttt tccgtctcga
    27181 tcataagccc cttctaattg atttggcagc agttgctcca ccccataaaa atttttctga
    27241 aaaacgtgat tgcatcctga tttcctcctt attttacaat tgccagaaca tcttcgtttg
    27301 caataatctc tttttggtct aattcaagta tatcggcaaa tgcatcagta ttcgtaatga
    27361 caactggagt gattacgcta tagccagctg ctctaattgc ttcgatgtca aaacgaatca
    27421 ataaatctcc tgctttgacg agatcccctt gtttgactaa gatctcaaag ccattcccat
    27481 ttaattctac tgtatccatc ccgatatgaa tcaaaatctc cgctccgtca gtcgttgtga
    27541 tccctacagc atgtccagtt ggaaaaatag tcgtgatctc tccgtctgca ggtgcaacta
    27601 gttctcctac ttctggatca atagcgatac cttttcccat cacgccagaa gaaaaaactt
    27661 ggtctggtac ttcactcaat ggcagtactt tacccgttaa aggacttttt aaggtatgcc
    27721 gtgctgaagt aatcggttct cctgcattcg catgtttgtt ttctaattga ttgtcttgtg
    27781 tttgttcgtc aaatcctaaa atcaatgtac ctacgaatgc aagaacaaaa gcaattcctg
    27841 tagctattac tgctactgtc acatttgact ctacaccatc caccgtatta atgaaagtcg
    27901 gaatcgtcaa taagctgacc aaactgctgg agaaagcttt tactccggaa aatccgataa
    27961 tagctccacc aatacctcca gaaatacaag cggcaatgaa tggtttcttc aaaggtaatg
    28021 tcacaccata cacagttggt tcagtgattc caaataacga agtcactgta gaagaaatcc
    28081 ctaatgctcg aagtttcgta tcttttgttc gcaatgctac agctaaagca gccccacctt
    28141 gtgccaagat ggctggaaga agcattggca tcaatacatc aaacccatat tgttcgatat
    28201 tcaaaaacat gatcggtacg aatccccaat gcatgccgaa catcacgaat acttgccata
    28261 aacttcccat gattagacct gctacgattg gactaaatcc ataaatcgag ttgaagagac
    28321 cgcctaaagc attaccggca accgtcccga ttggtcctaa agcaagaaat gttaatggaa
    28381 ccatcactac taaaacgaca agcggaacta aaatcatttg cagaaattgc ggaatcactt
    28441 tttttacgaa tggctctaat ttactttgaa cccatacggc tagaatgatt ggtatgacag
    28501 aagaagtata accagatggt gaaagaatca ctgggatccc aaagaaactg atagcttctc
    28561 ccgcaccagc aactgctgtg atagctggat acacaagtgc catagcaatt gctactgcta
    28621 aaaactggtt ggctttgaac tttttagctg ctgtaaaagc aagcatgaca ggtaagaagg
    28681 taaagacacc atctgctgca gcaaacaata tttgataagt accgctatca gctgtcagcc
    28741 agcccatcac cgttgccaat gataagaatc ctttcaatac cccggcggct gccatcgccc
    28801 ctagaaaagg tgtaaatata ctagagataa tatcgatcag acgattcaaa atatttttgg
    28861 actcttcttt tccatcctcc gaaactgcaa gaccttcctc atccacgatc gattgataaa
    28921 catctgctac attactgcca atcactactt gatactgacc gccactttgt acgacttgga
    28981 tcacgtccgg gtcttctttt aacttttgcg tatctgctaa agattcatct tttaatctga
    29041 atcgcaaccg cgtagcacaa tgtactaaac ttttgacatt cttttgtcct cctaccgctt
    29101 cccatacacg acgaccaact gcttgattat ccattgtttt ttccttcttt cttttttatt
    29161 taaaaaataa aaacccagat tgaaaaaaac agtcacaaac attcgtgaac ctattttttc
    29221 aatccaggtt ttgcctgcca tacagtaaca atccttatga ttggatgctt tttttctcac
    29281 ttactactct ttgaatatga atcgttaaat aaatctgctc tgcgatcccc atttcctgtt
    29341 tttgcgtttg tcgaagatac tcattgatcc gttcgacaca atgaaacgct tttggatact
    29401 ttccttgaac taatgcgtag agaaattcat cttgctcgtc agatccttcc cctttgagat
    29461 aacgctgggc aaaaaactgc agatgagtaa tgatacgctg ataattcaaa gattcttcat
    29521 cgaacacgat gccgaagaat ttactgatga tactcagtat atctcttacc attgtggtag
    29581 cggtcatcgt cacttgcatg tcttcgtttc cttgttggct attcacaaaa tgaaaggcaa
    29641 taaaaccggc ttcatcctca gaaagttcca cacccgtctt ttgtttcacg aacaaaagag
    29701 cctctttcgc tgcttgatat tctttcggat aaaatttttt tgtttcaaat aataatggat
    29761 taggcagttg gatattttct tttgctcgtg tgatcgcata atgaagatga tcggtcaatg
    29821 tcagataaat attaggcgaa aattcaactt ttagtttatc ttcagccagt tcgactactt
    29881 gtttgaccca ttcgatctcc tctgccggaa tatcagccat cagctgctgg aattggtttt
    29941 tagcaaaaga atctcttaaa caaattcttt ttggaccgct tctggcgaaa tctcgtcccc
    30001 aatctttttt tgaaaagcta gtccttttcc catacaaacg acttcttccc cgaatccatt
    30061 tttcgaaatg acgacattgt tattcaaaat tttatcaatt ttcattttgc ttcgccctct
    30121 caagaaaaat aaaaaaacct agaattcgcc tactgtttct tagctgaatt ctggttttgc
    30181 ctgccgtaca gtaacaatcc atttcttatt caaaatcatc ataccatcga atttttattt
    30241 atgcaagcct tttcatataa aaaataaaaa aactggaaca caagtctatc tagaaaacaa
    30301 ccaaatcaga gatgcacctc tttgcgtttc tctttttttc agcaaattgg tttttcctta
    30361 tcgattcata tcctttggct gtttttaatc tgtaacgact tatgtcccag tatcctaaaa
    30421 tgtttttgtc tctagattga aagcagatct catatcatct gtcgcatctt ttatcgatgt
    30481 aacactttcc agttctcctt gtacaactag tcgcgtgacg gcgtccatgt cgccacaagt
    30541 aattagtgtg acaatttctt ttccctcgac ttcattcagt aattgggtat ctgtaggagc
    30601 aactttttct ttaaaaaatg ttttgtatgt atacacattt tctaaatctg tcaaatagat
    30661 cttggcaccc atctctaaat tttctagcgg tgtaaacagc aattcagggt tcgttgcacg
    30721 atggcttgct aaggcataat ttccttttcc catttcttga tctggagata atgttcctgc
    30781 tccgtaaagt agtgacgtat tatccaagcc tttgaaaacc ggtaaattga ttgaaactga
    30841 agggactgcg atactagcaa tcactggtag atctgttcct ctcatttgcg acctcatgac
    30901 cccttctgtc gtcattggtt ctaccgcatc aaagtcaaaa ctgacatctt tatcgttatt
    30961 ctttttcaga tcttcttttg ttacattcgc tatagcatat ttatctcctg tctctctgac
    31021 aaaataatcc ttgatctgtt cattgaaaat caaagctagt cctactaata acaagaggaa
    31081 caagaggata ttgactagcc atctttttgc ttttcctttt tttttcattt tctctccctc
    31141 cagactcatt ttactgtttt tcttttgctc tttccaacag ccgaatgaca catttctaca
    31201 actctgtgta tttttttcgt acacctgcgc ttttttctac aggatatttc aaattatttt
    31261 tgatcaattt ttcttcaatg atctcatcta gatccaggtg aaggttgtct gccatcatgt
    31321 atgaatagat caatacatct gccagttctt ctttgattcg ttctaattgt gtttctttta
    31381 cttcttccgg cgttttccat tgaaaaagtt ctaacaattc agatgcttcc aaagaaatag
    31441 agatagccaa atctttttcg ttatgaaatt gtcgccaatt acgttcatct ctaaactggt
    31501 tgactttttc catactattc atttgcttgc cttcttccaa tcacttctta cttgattaat
    31561 cataccattt ttttacataa aaaatagatg atgcttttaa aaaaactcct gttttctttt
    31621 atttaaaata aatgtcatag aaaatcaata atttaggtag ccaccatgtg tatgcagtag
    31681 cgtgtcttct tcccttgagg ctttttgttt ttcgatacgt tcttcttccc aaacagacaa
    31741 cgctactaga atctctctta atgattttcc ttcttcggtg agcgagtact ctactttagg
    31801 cgggacttgg ttataggctt ttcgatggat actttcatct tgttctaact ctcttaactg
    31861 ctgggtcagc attttttttg tgatcgtagg gatttgcttg cttctccacg cctaaaataa
    31921 gcgatattgc tcttcccttt atctggtgta agcaaagcag ttagcatttt gacgatcgtg
    31981 ggttccgctt ttcacttctt atctacttag tatcatgaag tatactttcg tactaatact
    32041 aagtagcgga agtgtcaact actttgttta actacatagc aaaagaaaca gaagggtgac
    32101 aacggtcatc acgcttctgt ttcttttgaa tatgctgtgt tgttgtctct atctccgtgt
    32161 agactttcct caaacttatc ctttgacagt aagtcagtag gctgatttgc tctttctaga
    32221 tgccagatac atttctacaa aaaaatagtg attctgtttt gaaaattcga tttttagttc
    32281 tgcattttcg taaacatcca acattttttt cgcactgtat agacctaacc cgccattttt
    32341 tttagaggtt tttcctcttt tgaccatctc cgatagcgtc aaattcgttg gatttgcctt
    32401 gttcgctata ctaaaacgtg atccctcttt ttcttccagc agattgatcg taatcggact
    32461 ctgatcggct acacgatgat cgaatgcgtt agaaataaaa atagataaac agcgaataaa
    32521 aacaaccaga tcaatcggga ttgttttcac ttcgacagga ataactactt ggatcgggat
    32581 tttctccgat tctgcttttt ctagaaattt taaaaagaca cccctgacag ctggggatac
    32641 aatatttgtt agctgttgta tttgcggttt ttcaaaaata gctgcagaat gatcttctaa
    32701 tatttctaaa aattccaacg tttctttttt attttcctga agagtaattt ttaatgaaaa
    32761 cagtagactt ttataatcat gtttgaattc ctctagatga tggtaatatt cttcatttat
    32821 ttgatacagt tgaagcgatt cctgatgttg tctttctttt acttggaaac tgatcaacat
    32881 aatggaaaaa aacaaagaaa aaaaactgat gactatcgac aaaagagata gaagcagata
    32941 attagatgag cggaagtgaa cacttcccaa taccaagtaa atcacgcccc agcttaataa
    33001 aatacaaata cctccaaaat agtgttcagt tggataagta aaaagcaagt tgagaaatct
    33061 cgtctgtttg tctatacttt ttactaatag atagaaaata aatgcgataa gtagtgcaaa
    33121 aaaacttgct gttccaagat gtctctttcc taaataatcc atcagatcaa aaaccagaaa
    33181 aaagctgagt ccagtgatag caaaaaaaag attactgatc aaacagctga taaacaggtt
    33241 tttcgtagta aaataaaaaa gaaacatcaa taataagcca actaggtaag gaaagctcac
    33301 tttagggata ggaagtaaaa gtgtcaccac catcaaagct atacaaccaa agctgttttt
    33361 tactagcagt tgtttcgaaa agaataaata gatacctaaa tctaaaatca aagtaaaggt
    33421 caaataaagt gtcactgttg cgaccatctt attcctccat tgttttgttt caaaatgtac
    33481 acatattttt ttaataggag cctgcatatg aaaaagtatt ttatcataga agataaccag
    33541 tttcacagcg aatggatcat cagagaattg gtcaatcatc atatcgagaa tatcgtgcag
    33601 ctcacttcta tctctcaaat caatcagttg atccagttgg gcactattga taatcacgat
    33661 gttttcttta tcgatatcga cttgaagcaa tcactcacag gtattgatgt cgccgaacaa
    33721 atacgtaaaa aaaatgcaca tagtaatatt gtttttttca ccgcttatac gaattttgca
    33781 gtagagatca tcaatcggaa aatccttccg attggatata tcagaaaaga caggaattta
    33841 tctgaacaaa ttcgtcaagt gctagaacag atcaattgga aagaacagca gatcgtaaat
    33901 gtcgataaaa tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc tcgggaaatt
    33961 ttatatattg aaagtatcaa aggtttgaaa aaccaagtta tggtcaaaac aactacagaa
    34021 gataaattag tgaatacttc tttaaaacaa ttgaagacag agttagaaaa gtatgcttac
    34081 tttttacttt taaaatctta ctgtatcaac ttatcgcaat tgcagaaaat tgattctgct
    34141 cattatgtgt tagaattttt caacggtgac tccttactcg ttggtcgaaa gatctttgaa
    34201 aagacaaagg aacgtttcca tgacttccaa aaaactgctt cgagttgatg aataagagct
    34261 gagacgtaag catagcagat ttcagggagt gggccttgaa tcggctccac tccctgaaat
    34321 cgttgatatg attttacgct tcctcttctg taggagcatc tgttaacgac catgtgaatg
    34381 ttgaaacgta gttgtctaca gcataattgg atacaggaac aaataattcc actgattgtc
    34441 tcccttgttc cacattctct cctaatgaat aaatccatgt gccaatccct tcatttgctc
    34501 ctgcagttac tagatctact gcctgatctg ctgggatttc gattccttca gcgccagttc
    34561 cagaaacggt agatggcatt ccactgctat tagttgcgct atttcgaatc acggcgtttt
    34621 tgaatttcac ctttgaacca gttagtttat tcgtatcaga tgttgtacca ataaattctg
    34681 ttcgtttgac tgataatttc caaccggcat aatttccgct tttatccgtt acttgtacga
    34741 aattctgtac ttgtgtttgt gctccagtac tgtccgttcc gctttgtaat gcagcatata
    34801 ctgtttttgc tgtacctaac ttgattgttc caaaatcaag atctggagct tggtcaatcg
    34861 tcagtaatcc ggatgttccc tgatttggtt cgtcaggtgt aaaaggagta tcaggatcat
    34921 ttggatcaac aggaggaact ggaacaacgt cttcaggatt tgcagcatct ggtttggtca
    34981 cagaaatcgt tccttctgta gtggctgagc tcttattctc agcaaaaaca ctttgtgtac
    35041 ataataatag acttccagct agaacggtac ttaatgcaat tgtttttttc atttcttttt
    35101 ccttctttct tttaaaataa ataatccagc aaataagcca ataagaatga ctccaacaag
    35161 tatcagccat cgccagtcaa atgtttctgg ctgatttttt aaatcaatat ccttttcatt
    35221 caattggtcc gctgcatcat cagttatttc aaaatcggta tggagattcc agcgtttttc
    35281 tttccaagca cccttcacaa ccgcttgata atgtcccgga gctaattttt ttccttctaa
    35341 tgggacaagt aaatcaaaat ttgtattggg tgctacatct attttttctt tttttattga
    35401 aaggtaaggc tcttttgttt cttttttgta gatttcaacc gatgtagtca agccgaacat
    35461 ataagaagca tttggatttt gaagattcgc cttaatcaca tttcttccat cgctttgatc
    35521 cgcataagct ttttttaatt tcaaatctgg ggaagactct ttttcgctat ttcaaatcaa
    35581 gattgctttc acttgagaaa actgattgac gacaccaact gtacttttgt tagaaacgtt
    35641 ctcttttttt tcttgaaacg tgatccctcc tgcgaccaca ccagccagct ctttttctgg
    35701 cgctttcagt gaaagtgtct cagtgaccgc actttgagga ggaacagtta tttctcccac
    35761 tgtttttgct aagtttttca tatcataact taatgtttca tcaaatttac tttttgattc
    35821 tccatattcg atcacaccgt ttgaattcgt cgtagcggag tggatcaatg tctcaactgt
    35881 tactgattta tctgttcggt taaagaccgt gtaggggatt tcctgtgttt tcccaggctc
    35941 catccttaca tttatatatg ttttgttctt atcaatctga ttagccggta attcaaaatc
    36001 taccgcaaaa ttgaattcgg aggcatggac aggtgtctgc caaaggatga atccaaggag
    36061 aaggatcata agtaaagagc agttgtttcg atgactttta ttccatattc gattcatcaa
    36121 tcatcctcct tgatctttat tctgtcggcg catctgctaa tgtccatgtt atctctgctg
    36181 agtaattacc agctatctga ttttctttgg ttaagacaag tttaaacgcc cgaccgttat
    36241 ttaaatattc tgaataatct gcttgcacat tcgttgaatt ttgtcctctg acaatttcca
    36301 gttcttcttc attcactaat tgatactcct ctcctttctt gaaatatagg acatcctgca
    36361 tagaaaatcc tgcatcattt gtgaaaggct gagataattt tatcgttaag atccacggat
    36421 ttttcgtttc tcgctgatca taaatccata atttttcctg tatatccatt tggccaaatg
    36481 cttcttgtgt caaaccagtt gctttgatct gaccaaaatc gagtttggaa ggggcaagaa
    36541 atgagagacc accttctact acgatcttcg tagcaggtgc aaaaggcggg gcatcatgat
    36601 aaacaactgg cgtatcactt ggattgtgat tcccaatttc atcgttggta cttggcttgt
    36661 tctgcacacc cttagcagta gcttctcctg cttggtcgtt taatacaatt tctaatacat
    36721 ctcctgcaaa aagttgattt tctgggatag aaagaacaaa ttcccctgta tcatcaatag
    36781 ttgtttctga caaaacagct tgattttttt tcaatgaaac caaggctcct gcttcacctg
    36841 tacctgtgat cgtgcgagaa gctgcattga tcaatgtggt acttaaatta gcaggttcag
    36901 gaggaatcac atcgactgtc gttttggcag agtccagttc tgtcacttct tggccacctc
    36961 ttactttttt agcagcaaca acttgcactt gatcctccgt ttttaatctc tcatcattgg
    37021 gtacttgaac ttcgaacaag ccgccttgtt cgccttctcc ccatgggtta tatcctgccc
    37081 cttcattttc atctgtttgt ccttgcgtga gagcagtaat ttctacagat gatttcgtcg
    37141 tatctgcgta agtaatacgt aaagtcactt ctacttcgcc tgtccaagca tctcttggct
    37201 ttgcctcgac accttcggga acaacgacat gcccatagat ttttttatct gcattcgttg
    37261 gcactcttaa ggattcaatc atcgggagct gattattggc actgattcga ttataatttt
    37321 tcattccggc acctgtctga gcaaattcat ttgttaaacc tgcactaggg cttgatacga
    37381 aattgcctaa gtcggtccca ttgatcgata agtctgtcat atcagaaaaa taatccgggt
    37441 ctttttcaaa gttatcgaat gttgcgcctg tttttcgcca taaagcgatt tctgaatggg
    37501 cgatctttaa agaagaatct ccatttcctt ggaataccca gccgcctatg ccattggtct
    37561 cgcttccaat ccgtttgttt tggaaatcaa aataggtagg agattctaag tccacattca
    37621 acacatcccc actgatgatg ccggtcttat cttcccctgt tttcccgacc atgactaaat
    37681 aagttttttc acctgccgta aaatttaaat caatatctcc agttgtatct acagttgctc
    37741 cggaatctgc gcgtatatcg atatttgatt tttctccagt gactgtaaaa tcagctgaac
    37801 ctggactttc tagaaaacca gaagcgtcat aaaattggat cgcttgattt ccttgattga
    37861 gtctgttcac gtaagatttg ccatcacctg tatttcttac cctcaatgag cctccattgg
    37921 ttactgtcag cttttgcgta ccattctcaa aacgaactgc gctggaacgc ccactctgct
    37981 tatcgatgtc gataactgat ccggctcctt ctacgatcaa agaaaggcct tttcctacaa
    38041 aacgcatccc attgttcccg ggttgatcgt catctccagc cattttgacc gagatttgtc
    38101 caccacttga tacatggatt tgactagaat ctccttgcaa gcgaaatccg cccgtagttg
    38161 ctgtattgat ggtgattttc ccaccattcg atacagtgac tttcgatcct gaacctctca
    38221 tttggatgat tccgcgattg ttgtcattgg taatattgat ctttgtattt accgaaaatt
    38281 cgcttcccga accactgact tcaaacatga cattaccagg tagcgtatcg actgcctctc
    38341 cttcatcgct aacgccatct gcccgatcat aatcaagaga gacctttgaa ccgcttaaga
    38401 tatggattcc tggcgctgtt tttccactac cagtctcatc tcctgaatct agaaaaccgt
    38461 agaatttccc tagatacggt ccataaagtt gactaccatt gatcacattc aattgatagc
    38521 ttctacaaaa ataggcttta cccggagcgg cattcagtcc ttccttcact ggttggacat
    38581 ccaacacacc ggaaacacca tctaatgtga cagttgcacg aggagcgtaa atcagtccta
    38641 atttggaccc atcatacaaa tcaagtgtcc cggtaaattt tatttctccc gtaaaatgat
    38701 atctgcttcc ccaaaaatcg gtcatataag caatgataat ggcatttcca tcgatatttg
    38761 gtgtcaatcc acctggatgt gcagctgttc ctttaaaatt ctggaactcg atcgactgag
    38821 gtatctcatt gatataaata tggtgtgtgc cgaaattgtg agcgaatcca ccaaaatcaa
    38881 taacgatatc tcgtttgatc tctaattccc tgtctaaagt caaaggctga atcagtcgga
    38941 tcttagttac tgattcgtct gcgagaagct gcgccagttc ttctgttgtg gcagcttcgc
    39001 tgtacccatc cgctgaaaca gctcttgcca gtgccgcttc actgctatca gacgtttgtt
    39061 cagctacttc tgaggtctca cttgtcttat tttcttcttg ccttttatta gtagaagcct
    39121 cactttgaga ttctgtagtt tcagaagtaa tcgattcttc tgaaaaagta gtcttgtcaa
    39181 aactttgatt ctctgattgc gttgattcgt ttgttttctc tacatcggca aaaacggatg
    39241 tcaccatcaa gagagggaag acaaacaaaa tcgacgcaat cagctttttt acacattgat
    39301 tcattctcca ccatcctctc tgacgttttg acaattttat tttatcgttt taatctattt
    39361 ccaattgtac tttcatattt aaaatatgta tttttatgga aaaatttttc tattaggtgt
    39421 aaatttcttt tttgtacaaa aaaaaggctg tgacaaaagt cttgtcacag tcccttatcc
    39481 cgaacaagcg gtgttctccc gccaactcct cggtcacaac ctccatttta acaataaacc
    39541 tgtaaatgtg agggcaatac tttgattgtc acaggtaatt tatccccttc atctccatca
    39601 acattcgtcg tcaattcttc ctcatcagct aatgacacaa ctaattgctc acaagtaagg
    39661 taggctagat ttttcgtaga ttcatctact ccttttaata ggtctggcaa tgttttaagc
    39721 gtatcgatca aagaagaatc ttttaaataa accaaatgga gcttaccatc atccactttt
    39781 gcatttggaa gtaatgtttc aaaaccgccg actgagttag ttagtcctat cagcaaggta
    39841 ctgctctcaa tttcttcctt ctttccatca acttctacgt gaaacgagta ggattgtgtg
    39901 gaagctaact gtttgattcc tgacatgaaa taagctagtt ttccaaattt cgtcttcttc
    39961 tccgcatcta catcattgat tgattcaggg atagagccaa ttgcgacgac attcataaaa
    40021 tagtcgtcat ttatttttcc aatatccaat ggcttgactg attcaatcga aaaatgattg
    40081 atcgcttcct caggttctag cggtatccct aatgctcgag ccaagtcatt caccgtaccc
    40141 aatgggaaaa atccgaaatt tggccgatgt tcttgttcag cgattccact gattccttcg
    40201 ttgactgttc catctccgcc catgacaaac acactatgat acccttccgt agccgcttcg
    40261 cgagtaaagt tttttgcatc ccctgctttt tttgtatgca agacgaccac ttcatcaaac
    40321 accgattcca attttgctat cgctaattgc tcaaattcct ttgcttgttc accgcctgaa
    40381 ctggggttta ccactaataa aacttttttc atcactttct ttcccttctg attgatttct
    40441 ttaatataac gatagcataa agaaaaaaat taggctgtga tgaagtactg ttcatgaaac
    40501 attcatttat tttttgacaa gataaaacat ccatactatg aaactgagga aaattaaaac
    40561 aatgatatag gaaataaaag aaaatgatag gcattgtata cctaataaag taaaaatctt
    40621 aggtggcaga taaaaacaca aaaatagcaa gagaatgtaa tagcgtttct tccacttttt
    40681 gttatcagac cttatagcga tcagaggaat accaaatgca taactgaaaa actcttgatg
    40741 tactgttaat atgtcacggc cttgaataat aaatgcgaca atccccgcta taattagtgt
    40801 gcctaacacc aaaacggaaa attgatgaat atattttata tacaaatgat ccattgtact
    40861 tcctgccttt ttatttgtga gaaaaacaaa tccgtatacg tgaataactt ctagaaataa
    40921 aagaaa
//
LOCUS       DAYYHS010000016        39446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000016 DAYYHS010000000
VERSION     DAYYHS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.016"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1177
                     /locus_tag="ACHA6A_000861"
     CDS             134..1177
                     /locus_tag="ACHA6A_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8969084.1"
                     /translation="MSEFKYYKSKEVYREKYYQMPKVFFTNEKYLNLSNDAKITYMLL
                     KARFDYSVQNNWVDSEDNIYFIYTIEELMRLLNCREGKISKIKKELEAAGLLKQKKGR
                     IKKANGKIETTPNLLYLGKPEVTSEDVFKINEEERLIPNPVNAKIANTEKDDDIKVIP
                     VNAKIANTEKASDTNSSPVNAKIADNILYSNNSLDTNRHNIDTDKDQLQDQLLLENFV
                     DIMTDSSFDTFIPTNVLKLIKTFSNSYSEAQQTVKTIHNAKHKAQESENTIIVYEEIE
                     YYGVDADKGLYMTLLKAYQKQKTEKVDNLQNLIFIYVKNWFAEKAIPAKLAHEQNTET
                     NPLPAVNLENWLD"
     gene            2049..2441
                     /locus_tag="ACHA6A_000862"
     CDS             2049..2441
                     /locus_tag="ACHA6A_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgR"
                     /protein_id="HFM8969085.1"
                     /translation="MELKPKNLKQITCEFVLFNRQHIKFSNRTIKRKITDFSGKEIIL
                     YAKKLSSEQELTCFFDQIKAIYFDGKIVACRGCSELPLIVSNHVFSKMKARQKLLRES
                     KVRYREMQKEEWEKRKSAARKKYKRISA"
     gene            3012..3188
                     /locus_tag="ACHA6A_000863"
     CDS             3012..3188
                     /locus_tag="ACHA6A_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="HFM8969086.1"
                     /translation="MGTKKKRIKIAVTEETIQKLQWIVEEDQKKNNKRIYPCDSLERI
                     INNEYVIRKAFRDK"
     gene            3211..3357
                     /locus_tag="ACHA6A_000864"
     CDS             3211..3357
                     /locus_tag="ACHA6A_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969087.1"
                     /translation="MVKVMGKHTGRVYYSCPSKKEANEWILAKKIFEPLVILVLPRKK
                     EVTT"
     gene            3354..3629
                     /locus_tag="ACHA6A_000865"
     CDS             3354..3629
                     /locus_tag="ACHA6A_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969088.1"
                     /translation="MRTSYHYRPLVDGFSQNFNLQEEQKMIRRFEKDILAEIKKRKDR
                     KLPVSQYIANFSPKEKRCFFGLLKRFERVKVYYQLEEGMSRQYLIMA"
     gene            3709..4038
                     /locus_tag="ACHA6A_000866"
     CDS             3709..4038
                     /locus_tag="ACHA6A_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969089.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVVLTDYEE
                     DTTNEKL"
     gene            4025..4366
                     /locus_tag="ACHA6A_000867"
     CDS             4025..4366
                     /locus_tag="ACHA6A_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8969090.1"
                     /translation="MKNYDPTIRMGTHTIKVSFQRWDYKGFVTFRRGGNCKGLSVLAL
                     DEEDLYYQPLTDNPIGFGVLPEDDEGNKWFKMTLTNDEGDELLVEDVWEELGDYIVGL
                     EIIAFVADKEQ"
     gene            4382..4672
                     /locus_tag="ACHA6A_000868"
     CDS             4382..4672
                     /locus_tag="ACHA6A_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969091.1"
                     /translation="MKFFKVKAPCYYALIAAKDEEECMDLYERVVTDIEDREEFVRCL
                     QELDRNEAIEKDAKTISEETLEPIGMEEATKEIFSIINEQKSCVLSIDSALV"
     gene            4687..5052
                     /locus_tag="ACHA6A_000869"
     CDS             4687..5052
                     /locus_tag="ACHA6A_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969092.1"
                     /translation="MKINTSSAVSLTTFRLKKGYQNELIKAKIPSFVQERIEYFAKYL
                     SKGMTFSDLYMFVAAESDEEGLATMFEEIFASPYLDMSDEFMKWCRDPNNWRQRQVDL
                     ANAVIHVYQQKTRKERMII"
     gene            complement(5412..5846)
                     /locus_tag="ACHA6A_000870"
     CDS             complement(5412..5846)
                     /locus_tag="ACHA6A_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969093.1"
                     /translation="MKKKSLIILSLSVVLISVLIIAFSLFRKTETSSSTTTTTSSTVV
                     NDSIYPSLDELKALLSAKSIDNNLQVLGKTQITTVLFQDKNIAVLSPESGEEIQLPIK
                     VTNKEKNLFEIPELKYAPKSLKIGDIFGLAKKENTYFAYKEK"
     gene            complement(5900..6145)
                     /locus_tag="ACHA6A_000871"
     CDS             complement(5900..6145)
                     /locus_tag="ACHA6A_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969094.1"
                     /translation="MENSNLNHYQTWVTSNLITRKQAAEITGQSFAAFSQTIKQGRIK
                     PFVEIGEGSGPGMVRLYLKSDIEAYAKQLQAKKQKQQ"
     gene            6291..7202
                     /locus_tag="ACHA6A_000872"
     CDS             6291..7202
                     /locus_tag="ACHA6A_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8969095.1"
                     /translation="MKILRVLAIGATIGGIALATAHEKVDAAEWTPRTVEQIKADMKG
                     NEYTIVWGDTLSGISQATNISVQKLADMNKIANVDLIYAGNKLIFNGNVATVQNNKGE
                     TVAQSVIQPQDKVDSTKPVGQPATSQGTTSNNTTNSATQNTTTPSTDNTVTGNTQTGG
                     QTTGNTGTTTPSTPENNGGTTQTPTQPSQPDNNGNQNGSTGGNTGGGQTTPSNPGNNE
                     GNQGGSTGGNTGGSTTDPSQPTTPEKPVQYKYTVWYYADSEPQYTIEKGQHVFDTEAE
                     ATAWIDNYADNCPDTGSWGVMSWEVKQ"
     gene            7426..10656
                     /locus_tag="ACHA6A_000873"
     CDS             7426..10656
                     /locus_tag="ACHA6A_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8969096.1"
                     /translation="MKKVSSLFKKIIQSKPIMMALFLVCSIVGIGFGSGNVHAAVPNE
                     FTMKPGETGNVYYDTSNVYMMYGTRNGQPWSDATAPMAVKPDDGGIDIMTFCIQPGVP
                     FVSSNNPGYQAVGANDIPHDAQVASAIWNNPILGICGDTNENDRIVTQSVIWELLPYG
                     IHVDDIPRIPNYAQRKALLYQKIADYNKVPEFNKQTIELTYGQSKVLTSSVNLNSFET
                     QVSNTAKVNWAIAPDGQSVTVTPTDPNVQSGTISYKRAYMEGTPIALEKQGSQRVVIP
                     AIKDPRKYLVNFKINTFGEFQIKKVDKETGKTVPGTRFKLEYSNLPNGVAGPVIPTPT
                     EVVTDQNGLSPKIKALNGVHVKATETFVPAPYVLGSAIGDSDVVEGDIVAGQTITLTQ
                     RNKKAKGQIIVEKSGVESGKDMWNGNYSLAGNVFEVRKDSPTGEVIRTITTEETGHAE
                     TAKDLELGTYWVVEKTASNGFANTFKPVEVKIEYENQTTAVIIKNAEGTNQEVTGSTV
                     LTKEDAETGSETQGRATFNGAQYGLFHEDGTPVKWSDNFKPTTSKGNKLEGDEVKFEL
                     SDEEQQASVEHLALGKYFWKELVAPEGYQLDNTKREFEVTYKDQNTQVIATESISKEN
                     VIKFTLDGFKYVKSQSGSTQSGYNGIELKLTPIEPTKGEARTVKTETDANGYDGYWKF
                     EGVPYGDYDLEEVKAPEGYELITPLTIRSSFDEEKREYTFTIIEKGSKEPLKVVTKTE
                     QEINEGSNVIHLSKLFLVDEHARVPEPPEITTLFTTLEGEQTFNPTVDQKLIDKVKNK
                     FDKLDIGKTFYYVTQFHKIDQDGKDTVIDTVESQHKVEKEVFSFNVEHDYKANTLKHG
                     EKLVATHIVYSDKEHTDEYARHFDLSNEKQTLEAVTPGMPTIETLFVTKDGGKTFDPT
                     IDNELIDEVKATVPKEDIGKTFYYVTQFHKIDKDGKVTVVGTDESEHKAEEMVYEFKA
                     VYNYKANTLKDGEKLVATHIAYTDKEHKNEYAKHFDLKNEKQTLTAKTPVTPATPTTP
                     TTPQKSIPQTSGSLVNIGSLIVLGIAAVAGAALYVTTKKKVNKNEK"
     gene            10704..11741
                     /locus_tag="ACHA6A_000874"
     CDS             10704..11741
                     /locus_tag="ACHA6A_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8969097.1"
                     /translation="MFLGKGEINIVKKIIKALPLLWLLSSLILAICGFYGYTVLLRPE
                     NTIKQAQQITHPIQSEKEIKEIQQTTIGKATYNENQIQPVTPTDYAEAQLSYEALVNQ
                     WGIGSLYIPSSGIYSKILAGMSNDNLMVGLGTYYPNQLLGKGNYVLMAHNLVQGGGVL
                     RNLPQSSVGSTIYATDFSKIYEYEITTNKIVNQSESKLLDIPQEGDSPLMTIFRCEGG
                     LHTANRALIQARYVRSYSAENGSHDIKQALGLETTRNKTVNKQRLNDQQATSTNRTEA
                     AKESITPDKDTKKAKQTNKIEWRFTEKKAIYSNFQVFSILVFQLANAYPILIGLVFLV
                     GLSSCILLNRI"
     gene            11828..13354
                     /locus_tag="ACHA6A_000875"
     CDS             11828..13354
                     /locus_tag="ACHA6A_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8969098.1"
                     /translation="MKKKHISKVASAVLLSQMLLTTPLQVLADEQLSTPTSQSSTMNN
                     QSEESTSSSDEQTGTSTTQTTTDSTESSTDQSTTDDTTTSSTDTTSTTESSEKPTEDS
                     TTDSTSTTESSSESKPDETKPSEIKPSESKPEKPNTGSSTNESQTQPTTEPSNNQQAP
                     SVAPNNQATATREEQQAKPAESESKPYHQTPTAPIQELVPSSKETAEGNIHFEKDESV
                     ESFVRKIGESARKVGQENDLYASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGK
                     GVSFATQEDLGDGTLYTTQATFRQYENYEDSLNDYAQLLKEGLTGNSQFYSGVWKTNA
                     KTYQEATKFLTGRYATDTKYDQKLNGLIETYHLTDYDKEVAGPQLSKEGYIVPVKNYT
                     ISSPFGTRGGEFHRGLDLAAAQGEPIYASKAGTVVKAEFHPSWGNYVAIEHEDGTTAL
                     YAHQQEYQVKVGDKVKQGQIIGYVGSTGNSTGSHLHFELCLDHSLSQSQLVDPETVLF
                     "
     gene            13533..13940
                     /locus_tag="ACHA6A_000876"
     CDS             13533..13940
                     /locus_tag="ACHA6A_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969099.1"
                     /translation="MTNRNEGITIYLTIGKEVKLNFDSENNLSSIRLSINKVAELFGN
                     KVVNEESIFYVSTVALVTKSNYKTFISVVGGKQVISGDAVYEEVMIDQERTSNKRGNK
                     NVYEQGYFEFFVSEEDKIPKEKLILPYNVKKRR"
     gene            13964..15118
                     /locus_tag="ACHA6A_000877"
     CDS             13964..15118
                     /locus_tag="ACHA6A_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8969100.1"
                     /translation="MNITVKELGEPIELEDYLEEYKFQVIFLLKNMTFIDRKDNRVRQ
                     LFNMELIRAETKWYSFYQKNHRPPDYNFLQEEITSFGVNRLPMFDRRNEGRVNDELSV
                     DNFVHYHIQQLKLEKILSPVFFEKNDLRFMERYAKNKAKAYFNERDQYIKGYESFRIS
                     RNKIMQRAGYEQLKYEFVSDNMVQGYFKEEVAKMAANDERVAEFVFDEGKSLMKSAIH
                     QLNSISISAATGTTKAVTNQIKRQRTTGKQRYNQLKKHGSLEHIDVSKTDLKPLVNEL
                     KKYKVDFAVKRNKETGKIHVFFKAQDRDTLNLALENVLQKFANEKDQNREQQQDKPNK
                     DIEKSSVDDKDKDKSLTKKLDKLEDKAQEINQQREQLKENTKNKTKGQER"
     gene            15115..16989
                     /locus_tag="ACHA6A_000878"
     CDS             15115..16989
                     /locus_tag="ACHA6A_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8969101.1"
                     /translation="MNITKKDLIFGVALAAFFFYLGTLFFMVYFSIPQNTDSSWINVV
                     NEAFDKFLEPKTFTISPFTFSKIAMKGGAIIAVIVLLIDLYIISGKKNLRMGEEHGSA
                     KWGTSKDIAPYVDKKFKNNLLFTNTERITIETKLKKNKEYERNNNVVVVGGAGSGKTR
                     FYVLPNLLQMHSSYVINDSKGGILSDTAAIMQQNGYRIKVFNLNTMKNSDGYNPFHYI
                     RRTEGDCEIDILKVVNNLMKNTSDPNKKGGDSFWDDAQRSFLAAIFHLLILKGKPEEQ
                     NLGIVADLVRRARVKEDDEEYISSIDLVFQELEQEQPSCFAAKQYQNFKMAAGKTAKS
                     ILISLGVRLSPFDVPDIRKLTSTDTLELDTIGDRKTVLYIILPDTHTTMNFLASMIYQ
                     QLFDTLVYKADNVYNGSLPIPVRCLLDEFANTGQIPDFENVIATTRSRGISVDVILQN
                     LTQISKKLYKDSWETIIGNCDSFLYLGGNEQSTHKYISTQLGKETIDVVTYNESRGTT
                     GSFTKNSQKQGRNLLDPNEVREIKGGKCIYMLRGTKPFLSDRFKLERHPLFKKLKETP
                     SGYMYQSPLDQEEFIANNDPIDFELSKNVDMKEFLKHTEIETYDFEDEEIETINLDEL
                     "
     gene            17006..17206
                     /locus_tag="ACHA6A_000879"
     CDS             17006..17206
                     /locus_tag="ACHA6A_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969102.1"
                     /translation="MGTFNAIIDILSKYAIVGGGLYAVWGAVVLGGALKDHNGPQMQS
                     GIWQIVGGALIVAAAALFKTIV"
     gene            17238..18086
                     /locus_tag="ACHA6A_000880"
     CDS             17238..18086
                     /locus_tag="ACHA6A_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969103.1"
                     /translation="MDGFLTWCVESILEVISFSSKTSHLTSNLENFSPQLYKYSTTIL
                     DSVSKPIAFSILALFFALELQRISNRVETNGGGQTGFEMIFKSLVRLGLCKLAMDQVS
                     LIMRAIMDITGYMTDKINHLGMGNKEVSDMLDAKKILAPILKEGLMFKVGVSTVLIIA
                     LLITIGSIVIVQVVVNMRFIELYAFLAMSPIPIATFPNDEWSSIGKNFLKSFTATALQ
                     GTLIFMVMQFYPYLLSSAFSDITNSGSASETFLMTLIGIIGNSVLLIFAVMATGRWSK
                     SITNAM"
     gene            18351..18722
                     /locus_tag="ACHA6A_000881"
     CDS             18351..18722
                     /locus_tag="ACHA6A_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8969104.1"
                     /translation="MAIRVKVPKEIKDYQEKLMGGMSVRQLVFFVLAVLISVGVTAYC
                     VLVLHMSVDAAGYIVIPLLIPIVAFGWIRKEGMPLEKYGKIILNYYSKTGIRIYQTKE
                     RSNLNDFRKKRFQKVKETTKR"
     gene            18670..21120
                     /locus_tag="ACHA6A_000882"
     CDS             18670..21120
                     /locus_tag="ACHA6A_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8969105.1"
                     /translation="MILEKNDSKKSKKQPKDKQAKKVLKILAKQKKQRGKNQRRDAPP
                     LSSQRSLTYKAMYENGVCELKEGLFSRTFKFSDINYAIADQSEQELTFSSYCELLNSC
                     DSDTSLQISIINRSKDKKQFEKDLSYELVDDGLDVYRKEMNHMISNKIKYGNSSLQKE
                     KYITFNQRAKTLEQAIPALNRLQGDLEDQFSEMGCEVEELSGIERLRVVNDILRPNKK
                     LHFEYEDLLFSGLTTHSAIAPDSFDFKYRKDRFKMGDYYGQVLYLRNYPAELSDKLIT
                     NITDIPTNLQITLHIEPLDQQKGLDLVKTKLSFMEQQKIDEQKKAARSNYDPNLIPME
                     LRRSMDEAQELLDQMQGDNEKLFSFTFLVMAVDKDERKLDDTIEQILTAGRKNDCEFA
                     KLNYLQEEGLNSTLLWGNNFVPINRTLTTASTAVFIPFTAQELMQPNGKYYGLNSVTK
                     NLVTLDRRKLKAPNGFILGTPGSGKSFATKKEMTAVLLSDPDAEVIVIDPEREYTPLA
                     EALNGEVIQISAGSKNHVNPMEINNDYADDDDPVILKTDFVLSLCEMLVGGNIGLSPT
                     QKTLIDRSCRFAYKKYFENGSGMPTLKDFSNILKDQPEEEARQLSVELELYTEGSLSV
                     FSHQTNVDTSKRFIVFDVKDLGKQLRSMGMLIILDQIWNRITSNRNAGRRTWVYIDEI
                     QLLFSNEYAENYFFELWSRARKWGAIPTGITQNVSTLLSSLNAERMLSNSDFVLMLNQ
                     SQRDRERLGELLHISSRQKKYITNAKEGAGLMYAGDSMIPLYDEFPKDTNLYKVMSTK
                     IEDVYKYQNEQREQGTIV"
     gene            21117..22964
                     /locus_tag="ACHA6A_000883"
     CDS             21117..22964
                     /locus_tag="ACHA6A_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8969106.1"
                     /translation="MNTDFRERIIRKQKTAKKFVVNRDNNQFKKKIVSTDHTKRAKKA
                     NHSGKIKSSRGTSYLIKKDSAKNVVKDVNSKAQKNGVEQGNATAELETSKTIGRGIKN
                     STKATKTVAKTTTKVAKKSKELIQTNASKKEFLTVVKKGSKKGLVNTSKAAKDTVINE
                     IQEFRGDSESQGINEAIQLKDFIDTGIKGAKKTKKAANTAKKSSKALTKFGKSSYKYT
                     QRIAEIGIRRVQQVLSKEVAKALIAKLAIPLTLAVLILSIVTAVISTVNIFNFGAGDC
                     DTSANYDNITTKDIESNAKSIYSIIRKEIPESTPQGISGALGAMQFESQLNPSAVNPS
                     SGATGIAQWLGSRLTSLKEFAHKKGKKETDLGVQVEFLLSELNSSYYQSSKKILAMTD
                     VHEACKEWVMKFEGLSQDSSQWYLDQRNSYADHWYATLGTSDPIAEGTISNGADSELN
                     TLTCDLESDGGDIMEIAKSWLGWFHYDQIHPAPDLGTDLKNPNKNGRTDCSGFVWLVL
                     NKAGYKVPANMQWFTGSMASDAKGSHQYLKQISETEAKAGDIVIVNQGAGAGSNGHTG
                     ILTEKWRGKDTKIIQEGGNGDSVNIEAFGTSFMSLLDGGDVVLARPIKK"
     gene            22985..23605
                     /locus_tag="ACHA6A_000884"
     CDS             22985..23605
                     /locus_tag="ACHA6A_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969107.1"
                     /translation="MLNRKSVTLIVLVLIEAIFCFSIGLSINQELTVNSKITALEKKN
                     DALKRETKDQKSRISKLEDENNKMLETQVVNKDGDVYKEFTNIATKYFKSRFNYDPKT
                     YKESKENVRDLISEELYKKFSENQLTYGDSNNVSSRLDNLEIYSGSLISQKTITGLLI
                     VDYESKLGETDWFKNSEIYTVQYDIHAKKLTKIQSLGSVLRGDMIE"
     gene            23598..23972
                     /locus_tag="ACHA6A_000885"
     CDS             23598..23972
                     /locus_tag="ACHA6A_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFM8969108.1"
                     /translation="MNKKIFSICVLIFTVSIIFLADHSYIKYIEAQNDPVYLDESNQE
                     KIVFYRNDCRDCEKIMPYLIRHNFLYHDLTFVNMNNKENKRYIKEYGLTSVPTIISLK
                     QRYVGTDISKIKTLVMSKGDDF"
     gene            23972..24205
                     /locus_tag="ACHA6A_000886"
     CDS             23972..24205
                     /locus_tag="ACHA6A_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969109.1"
                     /translation="MARKSLTERIQQKKEEATKLEKRIQEDQYKLEKLTKQIRDLEAE
                     QVLAILDNHKLSVKDLNKILSKQPDNNNFINHN"
     gene            24278..24664
                     /locus_tag="ACHA6A_000887"
     CDS             24278..24664
                     /locus_tag="ACHA6A_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969110.1"
                     /translation="MLIAKRILASIVAIPCFLLSIIVIAVNIISIPILLVIQILKKVV
                     ILFITLVWIGFIGLFIYGLSSLGFSETLSMFTVPTIGILILTLILSIFTFISDKIPDF
                     LGIVSSFFLSIPKFLWGAEETEEAEE"
     gene            24685..25752
                     /locus_tag="ACHA6A_000888"
     CDS             24685..25752
                     /locus_tag="ACHA6A_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969111.1"
                     /translation="MFSKKKYVQFIEQRDIELPETPVEIYRGIYLLEQENDRLTDKHT
                     KGSTLILLTDKKGNELNRRLIQLPIEQGIETILTNLLIEPTSNKKHSSSKSQMILNDI
                     EEPKIKNKKKTVNYQTVFFLFTTNLAKKIYMALIIVLFLFFTSFFVLNNHSSTSTDSQ
                     SYHELLKKKDYEKIIKQYPEKENELVEKLYSEKNSKELKELAEKSDSQIALFYWDFLN
                     EHWQAVTTMKNVTPNETIQAMRGYAYLKQGKLDEAELINKEIDNQTLKDQIYQFKKQE
                     AYKALHEKDTEKAEKINKEINDSELSEDIKVAKSMVNLLQKYEKDRSDSKLSEDERKE
                     AENNYKMWSENLKQLGGNDNA"
     gene            25745..27331
                     /locus_tag="ACHA6A_000889"
     CDS             25745..27331
                     /locus_tag="ACHA6A_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969112.1"
                     /translation="MHNKKKFLVVGLFSAVLLGGAVTYGMYSQQPHNETEKTEQTTKS
                     ESSKHKSKKSAWEESVKGKEDKKDKNKHSTKKADDILAVIADTPLIKQQPNQNKNNLI
                     DRLAVALDDQTKKEADRQEKLIIKDKKSVDEPVLVDLSYKPQLEDTKPSNKENGKTEN
                     NKDENSESGKGNDTKPNNSLPDPLPVPEPNPLPDPTPSPDPNPIPTPDPDPDPDPEPE
                     PTPDPEPSIDEQINQSKEELNDSRKQADELNKQLTQVKDSLDNLQSIGASTEKNVDKA
                     ALQWQKVADLVTEYNQLTNEIKKLIEEDGTVLPINYDLYKETYEKLNQKVTEVKTAQK
                     EATNTTNEINKNVEQATDTLNNLEETKKEYQNKQKNVSEVDSNVNSAINHAQSNEQVA
                     SAVQDEINQAAQSNEQLNETNKTVGEQLNQVDPIESQTDIASAKEQTADINNQAEEQN
                     NNVDDMMNDFNNLPKPSDSEEPSVPENTDQNQDSTSQPQNIDNASVSQNKIENEQVSE
                     KTLPVTNQNSNQSEIEDIQK"
     gene            27350..28381
                     /locus_tag="ACHA6A_000890"
     CDS             27350..28381
                     /locus_tag="ACHA6A_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8969113.1"
                     /translation="MSYKDSEAKKNNKTAELLSQAEEGIKEVLSSDKYKNFLRVMSKF
                     HNYSLNNTLLILMQNPNATHVAGYKTWKNSFNRQVNKGEKSIKIIGGRPYKRKVEEIK
                     NGQKEEKEVEGISFFPVSVFDVSQTSGEPLPQLMNELSGEVKNYPELYVALKQSTNFS
                     IEFADIQGGTKGYCSPLEKKIVLNNGMSQAQTIKTLIHEITHADLHAPEFDKNSSIKT
                     TKSTKEVEAESTAFVVCEHYGIDTKDYSFPYLAAWSSDKELKELKNSFEVILKQADNL
                     IQKIDKNLAELQKEVTKEKEEKQSTLSLSDQLEEFEDKAKSLNEQRDKEKLINKIRQT
                     KEQKDVSTL"
     gene            28490..28798
                     /locus_tag="ACHA6A_000891"
     CDS             28490..28798
                     /locus_tag="ACHA6A_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969114.1"
                     /translation="MIEGALTVERINEIPDCLEKDHLFLIRKMLKEENFEPNDFIVVE
                     YPATGIYCCNHINNGQYFIIQEEKNKLVPYYTTCDMNEEGINNFPCTSIKESIKLIEM
                     "
     gene            28819..29088
                     /locus_tag="ACHA6A_000892"
     CDS             28819..29088
                     /locus_tag="ACHA6A_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969115.1"
                     /translation="MQTFTQHELEIISGHKQIAGSNANKLNVIKSEVGEMLSMLKSDL
                     GKQYIEDDEQRHYTLKNYQSVYDKLEKMSEDEFKQIDLSGIEELE"
     gene            29323..29736
                     /gene="mobC"
                     /locus_tag="ACHA6A_000893"
     CDS             29323..29736
                     /gene="mobC"
                     /locus_tag="ACHA6A_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8969116.1"
                     /translation="MEQKLAKTPENILQIAKTYDNDERNIQKKFRVSEREKFLIDYKM
                     SQANTRNFSRYARIMCLSGLMITTDFSDLKNLTTELGRIGNNINQLAHRANISNSVSK
                     EDFLELKINYDQLTKLINEELLFRMRRARDNQRFI"
     gene            29736..32030
                     /locus_tag="ACHA6A_000894"
     CDS             29736..32030
                     /locus_tag="ACHA6A_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8969117.1"
                     /translation="MAVTKIHQIKTTLNKAIGYITNPEKTNNGMLVSSYNCQSQFAEV
                     EMQMTREYAREVKGDYRKVGGSEVLAHHLIQSFSPEDKVTPELAHELGRQLIDELTEG
                     KFEYVIATHIDQEHIHNHIIFNSVSFYDLKKFRSQPYRTANKIKEISNRICAEHDLSL
                     SPKKEKLKDSYKSYSNRRNNTSFRSEIRKRLTMCLNESTSWEEFEEKADHLGVNIDRK
                     GKHISYALLSEGQQRRTRGDKLDDLETFTESNISQKLSANQSYINQLKEAIEETFFSS
                     DNLEDFSFLLEQEHGITFKSNKQDQFVFHFNDVNYFKWNESLLPDSYQIKNLKDYFRE
                     SYEFTNDSSLNSIEERYREKEKSVIVQEDTPIQLQEFQIEKATKEGLLVSIEHEDLNG
                     LVFVPNNYVDVNEETGEYTIWINDKFDYTLTDKQTNEQTSFTVKGEGIIRGLEKMQGI
                     QPQEIKLSSKNILSMSNRGVSISIPELNIKRLFIPSEYVVIDEISKSCSILIGEKWNY
                     YAEPLPENDDNSNKKLPYQKFTGRSLISSLKETPELLDVFLKPKFDQLHYQSRKRYTN
                     QLVEALNTIREEKIVSVSQLEQRLNEIVEQKNQAKEKIAEIDGKIKNYNNVAKLLVTY
                     EKYLPIMNEIERATMLQKIKLEQKYKEEVRQFKFAEKKLLASDNLRPDLTKEKIISVA
                     KNQEKQRKRLIEGYKHYENKLNRLIDVQEILQELDRNGLFDLSGYRLIDREEKLTDKI
                     EKYEKEVEETKQKNAKEKRKEHEI"
     gene            32169..32381
                     /locus_tag="ACHA6A_000895"
     CDS             32169..32381
                     /locus_tag="ACHA6A_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969118.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            32543..33061
                     /locus_tag="ACHA6A_000896"
     CDS             32543..33061
                     /locus_tag="ACHA6A_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8969119.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHTPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            33068..33580
                     /locus_tag="ACHA6A_000897"
     CDS             33068..33580
                     /locus_tag="ACHA6A_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969120.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            33851..34474
                     /locus_tag="ACHA6A_000898"
     CDS             33851..34474
                     /locus_tag="ACHA6A_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8969121.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYNEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
     gene            34742..35989
                     /locus_tag="ACHA6A_000899"
     CDS             34742..35989
                     /locus_tag="ACHA6A_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8969122.1"
                     /translation="MKNKLTLLICATILSTWAVPTQNAFAEETGNDTTQQTVTSNSSS
                     EEQTAIINGEEVKLEAPVKEGQSEAEATQAPKEELQQDQMSRFARSAVRTIPMYRMYN
                     PNSGEHFYTQDTGERDNLRRIGWNYEGIGWQAPTSGDPVYRLYNRYNGEHFYTLNAKE
                     RDNITKQGWTYEGIGWYSYKGANGIPVSRLYNKRVNWHHYTLDEHEKNVISKQGWNYE
                     GVGWYAVGNGQPSNDDYKLLGVKNYNQYALGAPSGCEGASLLQGLQYKGKITNWSLTQ
                     FLNTIPKSPNGNPNNGFVGSPFVENSWTYSAIYPAPLATWGQKYGNVQNISGSSMNTL
                     LNEVKNGNPVVAWVTINFQPIRWGNWSFGVAANNNHAVTLDGYNKGSNQVHVSDPISG
                     SYWLNRTTFENIYNARKYAVVVR"
     gene            36261..37586
                     /locus_tag="ACHA6A_000900"
     CDS             36261..37586
                     /locus_tag="ACHA6A_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8969123.1"
                     /translation="MFDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMKLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQERIKEELGLIA
                     AVGVGDNPLLAKLALDNEAKNNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIPPEPSKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIIHASSKLEGARSITRSTLVGGHAGGVGGLKND"
     gene            37579..37929
                     /locus_tag="ACHA6A_000901"
     CDS             37579..37929
                     /locus_tag="ACHA6A_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969124.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDAENEQRANQNLAKEQMTTE
                     EITEVLNAAVIKNRSVCIQKEERNAEGYYPDDIIGKIFGSDELGIYVGKNKVHYDEIR
                     HIEFYQKHKWSCLE"
     gene            37926..38120
                     /locus_tag="ACHA6A_000902"
     CDS             37926..38120
                     /locus_tag="ACHA6A_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969125.1"
                     /translation="MNIIKQYELKYITLENLSDEIWGYGQQLINEIGIDCFKFYVLIA
                     GGYLNFEYHILNYSDSHFSV"
     gene            complement(38206..38568)
                     /locus_tag="ACHA6A_000903"
     CDS             complement(38206..38568)
                     /locus_tag="ACHA6A_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969126.1"
                     /translation="MTNEKNESIGLNAIKRKNKTQRPENIVPENSEALNNTTDSGKIL
                     EQSKKRLTTKDLPKSIRMPLDTHTAISTIATIEDKKIYETLNKIVEFYINNMNPANKK
                     IVKNSIKAVQNIYKDTDN"
     gene            complement(38582..39388)
                     /locus_tag="ACHA6A_000904"
     CDS             complement(38582..39388)
                     /locus_tag="ACHA6A_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8969127.1"
                     /translation="MTKVLTTGNFKGGVGKTTNAVLTAYTLSNKGYKVLVVDLDPQAN
                     ATELLFATMTNVYKIKPEFKETLFVSIQNNKISNSLISVKENLDLLPSFTDLEKYIDY
                     LAELYDDDYSKDTHFSNLLNEIKENYDFIIIDVPPQLNKFTNSALVASDYVIVILQTQ
                     ERSLKGAEKYIEHLIQLNNDYGTEIDILGLLPVLVQNGNDLDLDIIEDAESLFGKSNI
                     FNTKIKTMQRLKRYDRTGITDNPKDIHDKRVQQVYQSVSDEVIQRINLLG"
BASE COUNT        15006 a         5672 c         7252 g        11516 t
ORIGIN      
        1 cagaaaagac cattgctgat tggattggcg tcctgcaata gtctttgagt aagtaattaa
       61 aagatttaat ttattatatt atagcatatt ttaattactt actccactac atgaaaataa
      121 aaggagtaat attatgtcgg aatttaaata ctacaagtca aaagaagttt atcgtgaaaa
      181 atactatcaa atgcctaaag tattttttac aaacgagaaa tatttaaatt tatcaaatga
      241 tgcgaaaata acctatatgc ttttgaaagc acgttttgat tactcagtac aaaataattg
      301 ggtagattct gaggataata tttattttat ttacaccatt gaagagttaa tgagattact
      361 aaactgtcga gaaggtaaaa tttctaaaat aaaaaaagaa ttagaggcag caggactgct
      421 gaagcaaaag aaaggacgta tcaaaaaagc aaacggaaaa attgaaacta cgccaaacct
      481 tttgtatcta ggtaaacctg aagtaacttc agaggacgta tttaaaataa acgaagaaga
      541 acgactaatc ccaaatcctg taaatgcgaa aatcgcaaat acagaaaaag acgatgatat
      601 caaggttatt cctgtaaatg cgaaaatcgc aaatacagaa aaagccagtg acaccaatag
      661 ttctcctgta aatgcgaaaa tcgcagacaa tatattatat tctaataatt ctttagacac
      721 taatagacac aatatagaca ctgacaaaga tcagctacaa gatcaattat tgctagagaa
      781 ttttgttgat ataatgactg attccagctt tgataccttt ataccaacta atgttttgaa
      841 attgattaaa actttttcaa atagttacag tgaagcacaa caaacagtca aaactataca
      901 caatgcaaag cataaagctc aagaatcaga aaacacaatt attgtttacg aagaaattga
      961 gtattatgga gttgacgcag ataaaggatt gtacatgact ttactgaagg catatcaaaa
     1021 acagaaaaca gaaaaagttg ataacctaca aaatctaatt tttatttacg taaaaaactg
     1081 gtttgctgag aaagctattc ctgctaaatt ggcacatgaa caaaacacag aaactaaccc
     1141 attacctgct gtaaatctag aaaattggtt agattagaaa aaacgaacat atgtttgcaa
     1201 tatggacgat gagtggtata attttattgt gatatatatt gcatgatcat ttaacaaaga
     1261 aaaaattaga ataaatcatg ttatttattt tctaaatttt cattaaaact ttcatgagtg
     1321 ttctttatta gataacgtta gtatttctaa taaaaaacgt gaaaagtgcg taatgtaagc
     1381 tttgttatca atgattttaa atgttaaaat aattccagaa gcgcttcaca actatgtaag
     1441 tggaggtggt gtatatcaaa atataattgt tttatgttgt tttacgataa ttagtactta
     1501 cacaaatttg tcaagcgaaa aataaaaaag cttgtaatga atttgaagat gtgctaccgt
     1561 aaatttgtag aaacgtaaat taaaaaaatt attgattagc aatccagact gctgatcatt
     1621 gagaagccaa gcaataaatc aaccaacaaa aaagaggaga ccgtctagtg atctcgctgg
     1681 gtaagagttg gcgctcttac ttcaggtcat gaatcactag ccgtccaatt caacgtccgt
     1741 aacacgctga agaggtaaac tcccactttt gttgcagatt taactgcaac aggcatattc
     1801 taacatatat ttcttaagaa tttaactagt ttatttgtgc tacctaaatt ctttaaaaga
     1861 aaatttgtat ttctctggct acttttttga gtagacttag tgtgcaattt tctagatata
     1921 tttgtttata ctgtttgcag ctgatttgtg acttaagagg gcaattacac ttcatgtgtg
     1981 attgtcctct ttttgtcgtg cttggcttcc caatgatcag caactgaagc agaagggcgg
     2041 agaaaaacat ggaattaaaa cctaaaaatt taaaacaaat tacttgcgaa tttgtattgt
     2101 ttaatcgaca acacataaaa ttttctaatc gaacgatcaa gcgtaaaatt actgattttt
     2161 caggtaaaga aattatttta tacgctaaaa aattatcaag tgaacaggaa ttaacttgct
     2221 tttttgatca aataaaagct atctattttg acggcaagat cgttgcttgt agaggctgtt
     2281 cggagttacc tttaattgtt tcaaatcatg tgttttcaaa aatgaaagca cgtcagaagc
     2341 tcttaagaga gtcgaaagtt cgatatagag aaatgcaaaa agaggaatgg gaaaaaagaa
     2401 agagtgcggc acgcaaaaaa tataaacgaa tcagtgccta gctactgttt tgactaatga
     2461 tacttgattt ttttgtttgt tagttttttc taacaaacaa cctaatagca gggagttatt
     2521 gctatcgaaa aaacggacaa gtaagcacac ggacacgaaa cagcgctaaa tatttattta
     2581 acgtttgctg gcaaggagtc acatgacacc aagcgataca cgtaaactta ctttgatgtt
     2641 ttgggtgttc cttgacaact gaaaaattac aggactttct aagttaatca gcgtgtctaa
     2701 taggggctta ggaaggataa accacctaaa aaagtacggc tgtgttatca gatggtacgt
     2761 tattgatggg tagggcacac acagaaaagg ttatttgcga tgatcagcta tcacgaaaaa
     2821 cataagatga cgattaaaac cgtccgctcg tttttctagg gagcaaaaac acttttcgag
     2881 agtttggggt tgctatcgct agaaaccctg cgtaaaaaaa ctgtaacatt tatgagaaaa
     2941 ctaataaaca aaagaatcaa gtatctaaaa aataagggga cacctactaa tagagaaggg
     3001 gacacctgaa tatgggaaca aagaaaaaaa ggatcaagat tgcggtcact gaggaaacga
     3061 ttcaaaaact tcagtggatc gtagaagaag accaaaagaa aaacaacaag agaatttatc
     3121 cttgtgactc tttagagcga ataatcaata acgaatatgt tatacgtaaa gcatttcgag
     3181 acaagtaatt aagagaagga gtgaatgcct atggtaaagg ttatggggaa acataccgga
     3241 cgtgtgtatt attcctgtcc aagcaaaaaa gaagccaatg aatggattct tgcgaaaaaa
     3301 atctttgagc cattagttat ccttgttctt cctcgaaaga aggaagtgac aacatgagaa
     3361 caagttatca ttatcgacct ctagtcgatg ggtttagtca gaatttcaat ttacaggaag
     3421 agcaaaaaat gattcgtcgt ttcgagaaag acattttagc tgagatcaag aagaggaagg
     3481 atcgaaagct tccagtaagt caatatattg cgaatttttc gcctaaagaa aagcgttgtt
     3541 ttttcggtct tttgaagcga tttgaacgag tgaaggtcta ttaccaattg gaagaaggga
     3601 tgtcgcgcca atatttgatt atggcatagg aactcgttcc atgagctgtg aaataaagca
     3661 attctataaa atacgaccat tatgggagat agcagaggtg gaaaaaggat gattcaaaca
     3721 tttgcaaaaa tgagtagaag tgcttggatt gaaaagcttg aaaatgactt gagtgtcatg
     3781 cgattagctc caaagaaatt caaacgagag attgcagaaa aagaagcaat tttgtcggta
     3841 ttgaatgcgg tagacgacaa atgtccagta aaaattagtt tttcagatat agccgcaatt
     3901 atcgaaggta tctactatgg acaagccgat gaagagatct tttctataca agatttatta
     3961 gaatttttga acaaaaataa ggatagcttt gtagtactaa ctgattatga ggaggataca
     4021 acaaatgaaa aattatgatc caactattcg aatgggcaca catacgatta aagtttcttt
     4081 tcaacgttgg gattacaaag ggtttgtgac atttagaaga ggtggtaatt gcaaaggatt
     4141 aagtgtctta gcgttagacg aagaagattt gtattatcaa ccattaactg ataacccaat
     4201 cggatttgga gtacttcctg aagatgatga aggtaataag tggttcaaaa tgaccctgac
     4261 aaatgacgaa ggggacgaat tgttagtgga agatgtatgg gaagagttag gtgactacat
     4321 tgtaggtctt gaaattattg cttttgtagc agataaagaa cagtaaacgg aggaatagcc
     4381 aatgaaattt ttcaaagtga aagcaccatg ttattatgcg ttgattgcag caaaggatga
     4441 agaagagtgc atggatcttt acgagagagt agtaacagat atcgaagatc gtgaagaatt
     4501 tgtacggtgt ttgcaggaat tagatcgaaa tgaagcgatt gaaaaagatg caaaaacaat
     4561 cagtgaagaa acgttagaac caatcggtat ggaagaagcc acaaaagaaa tttttagtat
     4621 tatcaatgaa caaaaatctt gtgtgttaag cattgattct gcactcgttt gaggagataa
     4681 gatgatatga aaataaatac gagtagcgca gtttctctaa ctacatttcg gctaaaaaaa
     4741 gggtatcaaa atgaattgat taaagctaaa attccaagtt ttgtacaaga aagaatcgaa
     4801 tattttgcaa agtatctttc aaaaggaatg acattttcag atttgtatat gtttgttgca
     4861 gctgaatcag atgaagaagg actcgcaaca atgtttgaag agatttttgc cagtccttat
     4921 cttgacatga gtgatgaatt catgaaatgg tgtcgagatc caaacaattg gagacaacga
     4981 caagtcgatt tagcaaatgc agttatccat gtatatcaac aaaaaacaag gaaagaacgg
     5041 atgattatat aaatgggtat tttttaattt ttttgggact agttggtttg gtgatatttc
     5101 ttttgcaaga tcgacaagaa gtatgtgaga gaaattctga tagcaaaaat ccatgaaaat
     5161 gagtgtgaag ttcacttaaa aagaaaaata actaacaaat ttcagttact ttcttatgga
     5221 agtaattgag atttgctagt tatttactag tgaaagcaaa tttcattttt ttctcctttc
     5281 tttttataaa aaaatcttac gaggggtgtt ccctcgtaag taataaacga ttatgatacc
     5341 aaaacaatat tttatgaatg aaagcgcaac attatatgaa aaatttgtta taataagcaa
     5401 tgaaactatg cttacttttc tttatatgcg aaataggtat tctctttttt tgctaagcca
     5461 aaaatatcac ctattttcaa tgatttagga gcatatttca attctggaat ttcaaataaa
     5521 tttttttctt tgttagttac tttaattggt aactggattt cttctcctga ttcaggagaa
     5581 agaacagcaa tgtttttatc ttggaagagt acagtagtaa tttgagtctt ccccaaaacc
     5641 tgtaaattat tatctataga ttttgcgctt agaagcgctt ttaactcgtc tagtgaggga
     5701 taaatagaat cattgaccac agtacttgaa gttgttgtgg ttgtggaact acttgtttct
     5761 gtttttctaa acagagaaaa agcaatgata agtacagata tcagtacgac actgagtgat
     5821 aaaattatga gggatttttt cttcataata gttattctcc atgataaaat tgttttttct
     5881 ataaagaagc agaaactact tattgttgtt tctgcttttt agcttgtaac tgtttagcgt
     5941 aagcttctat gtcactctta agatataggc gaaccattcc aggaccagaa ccttctccaa
     6001 tttcaacgaa gggcttaatt cgaccttgtt taattgtttg agaaaatgct gcaaaacttt
     6061 gaccagtaat ttcggcggct tgttttctag taattaaatt ggatgtaacc caggtttgat
     6121 agtgatttaa attgctattt tccataaagc aacctcctat ttattggatt ctaagataag
     6181 tataccaaaa ttaatatctt ttttcaaaat agttgttgac ataggattct aaaattggta
     6241 tcctatgttt gtaaataaaa taaataataa atagaaagaa ggaatataaa atgaaaatat
     6301 tacgagtact tgcaattgga gcgactattg gtgggattgc gttagcaact gctcacgaga
     6361 aagtagatgc ggcagaatgg acaccacgta ctgtggaaca aatcaaagca gatatgaaag
     6421 gtaacgaata tacaatcgtt tggggagata cactaagtgg aatcagtcaa gcaacaaaca
     6481 tttctgtaca aaaattagca gatatgaaca aaattgcgaa tgtagactta atctacgcag
     6541 gcaataaact aatttttaat ggaaatgtag caactgttca gaataacaaa ggagaaacag
     6601 ttgcacaatc agtgattcag ccacaagata aagttgactc aacgaaacca gtaggacaac
     6661 cagcaacttc acaaggaaca acaagcaata atacaactaa ttcagcaact caaaatacaa
     6721 caacaccttc aactgataac acagtgacag gtaatacaca aactggcggt caaacaactg
     6781 gaaatacagg aacaacaacg ccaagcacac cagaaaataa cggtggtaca actcaaacac
     6841 caacacaacc aagtcaacca gataataatg gtaaccaaaa cggttcgaca ggtggaaata
     6901 ctggcggggg acaaacaaca ccatcaaatc ctggcaacaa tgaaggaaat caaggtggat
     6961 caactggagg aaatactggc ggttccacaa cagatccaag ccaaccaact actcctgaaa
     7021 aaccagtaca atataaatac actgtttggt attatgcaga ttcagaacct caatatacta
     7081 ttgaaaaagg acaacatgta tttgacacag aagcagaagc tacggcatgg attgacaatt
     7141 atgcagacaa ttgtccggat acaggttcat ggggagtaat gtcttgggaa gtaaaacaat
     7201 aatttattag tttagtaaag taaaactcac aaaaagattt ttgtgagttt tttatttttt
     7261 taggaggtag cgagaataat gacactgaat tattacgctt tctatgggta ttattcatcg
     7321 tatttgagag agtttatata aaatgaattt aattttgctt ttaagacgct aaaaaatctt
     7381 tttagtgtct ttttttttat taatttttta ggagggaatc actcagtgaa aaaagtaagt
     7441 tctttgttta agaaaattat tcaatctaag cctataatga tggcactctt tttagtatgt
     7501 agtattgtgg ggattggttt tggaagtgga aatgttcatg cggctgtgcc aaatgaattt
     7561 accatgaaac caggagaaac aggaaatgtt tattatgata ctagtaatgt ctatatgatg
     7621 tatggaacac gtaatgggca accttggagc gatgccactg caccaatggc agtaaaacca
     7681 gatgatggtg gcattgacat tatgacattt tgtattcaac caggtgttcc atttgtttct
     7741 agtaacaatc ctggttatca agctgttggt gccaatgata taccacacga tgcacaagtt
     7801 gcttcagcaa tctggaacaa tcctatttta ggtatttgtg gagatacaaa tgaaaatgac
     7861 cgtattgtga ctcaatcagt tatctgggaa ttgttaccat atggcataca tgtagatgat
     7921 attcctcgta ttcctaatta tgcgcaaaga aaagcattgt tatatcaaaa aatagccgac
     7981 tacaataagg ttccagaatt taataaacaa acaattgaat tgacatacgg acaatctaaa
     8041 gtattaacat cttctgttaa cttaaattca tttgaaacgc aagtatctaa tacggctaaa
     8101 gtaaattggg caattgctcc agatggacaa tctgtgacag taactccaac tgatccaaat
     8161 gttcaaagtg ggacaatatc gtataaacgt gcatacatgg aaggcactcc tatagcttta
     8221 gaaaaacaag ggtcacaacg agtagttatt cctgctatta aagatccaag aaaatacctt
     8281 gtcaatttta aaatcaacac atttggagaa tttcaaatta aaaaagttga taaggaaaca
     8341 ggaaaaacag taccaggaac tcgtttcaaa ttagaataca gtaatcttcc aaacggtgtt
     8401 gcaggccctg ttattcctac accaacagaa gtggtgacag atcaaaatgg cttatcacca
     8461 aaaatcaaag cattgaatgg tgtacatgtc aaagcaacag aaactttcgt tccagctcct
     8521 tatgtattag gatctgcgat tggcgattct gacgtcgtag aaggcgatat tgtcgcagga
     8581 cagacaatta ctctgacaca acgtaataaa aaggcaaaag gacaaatcat tgtcgaaaaa
     8641 tcaggtgtcg agtctggcaa agatatgtgg aatggtaact attctctagc agggaatgtc
     8701 tttgaagtta gaaaagatag tccaacggga gaagttatcc gtacgatcac aacagaagaa
     8761 actggtcatg cagagacagc taaagattta gaattaggta cttattgggt agttgaaaag
     8821 acagcttcta acggttttgc gaatacattt aaaccggtag aggtaaaaat cgaatatgaa
     8881 aatcaaacaa cggcggttat tataaaaaat gctgaaggta caaaccaaga agtgacagga
     8941 tcaactgtat taacgaaaga agacgctgaa acaggttcag aaacacaagg acgtgcaaca
     9001 tttaatggtg cgcaatatgg cttattccat gaagacggta ctcctgtgaa atggtctgat
     9061 aatttcaaac caactacttc taaaggcaac aaattagaag gcgacgaggt caaatttgaa
     9121 ttatctgatg aagaacaaca agcaagcgtt gaacacttgg cattaggtaa atatttctgg
     9181 aaagagcttg ttgccccaga aggatatcaa cttgataata caaaacgcga atttgaagtc
     9241 acttataaag atcagaatac acaagtgatt gcaacagaat cgatttctaa agagaatgta
     9301 atcaagttta ctcttgatgg atttaaatat gtaaaatctc agagtggttc aactcaaagt
     9361 ggttacaacg gtattgagtt gaaactaaca ccaattgaac caacaaaagg ggaagcacgt
     9421 actgttaaaa cagaaacaga tgctaatggt tatgatggtt attggaaatt tgaaggagta
     9481 ccttatggtg actatgactt agaagaagta aaagcaccag aagggtatga attaattaca
     9541 ccattaacta tccgttcttc ttttgatgaa gaaaagcgtg agtatacatt tactattatt
     9601 gaaaaaggct ctaaagaacc tttaaaagtt gtgacaaaaa cagaacaaga aattaatgaa
     9661 ggatcaaatg ttattcattt aagtaaattg ttcttagtag atgaacatgc tcgtgttcct
     9721 gaacctccgg aaattactac tttatttaca acattagaag gtgaacaaac ttttaatcca
     9781 acagtggatc agaagttgat tgataaagtt aaaaataaat ttgataaatt agatatagga
     9841 aaaacattct attatgtgac acaattccat aagattgatc aagatggaaa agatactgta
     9901 attgatacag tagaatctca acacaaagta gaaaaagaag tatttagttt taatgtagaa
     9961 catgactaca aagcaaatac attgaaacat ggtgaaaaat tagttgcgac acacattgtt
    10021 tattctgaca aggaacatac cgatgaatat gcccgtcatt ttgatttgag caatgaaaaa
    10081 caaacattag aagcagtaac acctggtatg ccaacaatcg aaacactatt tgtaacaaaa
    10141 gatggtggta aaacattcga tccaacgatt gataatgaat taatcgatga agtcaaagcc
    10201 actgtaccta aagaagacat tggtaaaaca ttctactatg taacccaatt ccacaaaatt
    10261 gataaggatg ggaaggtcac agtagtaggt acggatgaat cagaacacaa agcagaagaa
    10321 atggtttatg agttcaaagc tgtttacaat tacaaagcaa acacattgaa agacggcgaa
    10381 aaattagttg cgactcatat tgcctatact gacaaagaac acaaaaatga gtatgcaaaa
    10441 cactttgatt tgaaaaatga aaaacaaaca ctaacggcaa aaacaccagt tacgccagca
    10501 actccaacaa ctccaacaac accacaaaaa tcaattccac aaacttctgg atcattggtt
    10561 aatattggaa gcttaatcgt tctaggaatt gctgctgttg caggtgcagc attgtatgtt
    10621 acaactaaga aaaaagtaaa taaaaacgaa aaataagaat gaaggggaag cactttgaaa
    10681 aaggtgcttt cctattttag ttattgtttt tagggaaagg agagatcaat atagtaaaga
    10741 aaatcattaa agccttaccg ttattgtggc tactaagtag cctgatttta gcaatctgtg
    10801 gattttatgg ttataccgtt cttcttcgtc ccgaaaatac aatcaaacaa gcacagcaaa
    10861 tcactcaccc tatacagtca gagaaagaga tcaaggagat acaacaaacg acaataggga
    10921 aagcaacata caatgaaaat caaatccaac ctgttacacc tacagactat gcggaagcac
    10981 agctgagtta tgaggctctt gttaaccagt ggggaatcgg ttctctctat attccgtctt
    11041 ctggtatata ctccaagatt cttgcaggaa tgagcaatga caatttaatg gttggcttgg
    11101 gaacctatta tccaaaccag ttacttggaa aaggaaacta tgttttgatg gcacataatt
    11161 tagttcaagg tgggggagtc cttcgtaatt taccacaatc tagcgtagga tcaacaattt
    11221 atgcgactga ttttagcaag atttatgaat atgagatcac tacaaataaa attgttaacc
    11281 aatcagagag taagttattg gatatcccac aagaaggtga ttcgccttta atgactattt
    11341 ttagatgtga gggaggatta cacacagcta atcgtgcatt aattcaagct aggtatgttc
    11401 gatcttactc agctgaaaat ggttcacatg atattaaaca agctttagga ttagaaacaa
    11461 cgagaaataa gacagtcaat aaacaacgtc taaatgacca acaggcgact tcaactaata
    11521 gaactgaagc agcaaaggaa agtattactc ccgataagga tactaaaaag gcaaagcaaa
    11581 caaataaaat agaatggcgc ttcacagaaa agaaagctat ttattctaac ttccaagtgt
    11641 tttctatcct agtatttcaa ttagctaatg cttatcctat tctgattggg ttagtttttc
    11701 ttgttggtct ctcaagttgt attctattga acagaatata atgagtgaca aaacttatat
    11761 atatttttat aaaaaattca taaaataaag ttatcttaga tgtaacacag gaaaggaaag
    11821 agaaaaagtg aaaaaaaagc atatttcaaa agtagcttcc gctgttcttc tttctcaaat
    11881 gctattgaca acaccgttac aggtattagc agatgaacag ctttcaacgc caaccagtca
    11941 atccagcacg atgaataatc agtctgaaga atctacttca agtagtgatg aacaaacagg
    12001 aacgtctact acacaaacaa cgactgattc cacagaaagt agtacagatc aaagtacaac
    12061 cgatgataca acaactagct cgacagatac gacgtctaca acagaatcgt ctgaaaagcc
    12121 tacagaagac agtacgacag attcaacaag tacaacggaa tccagtagtg agtctaaacc
    12181 agatgaaact aaacctagcg aaataaagcc gagtgaatcg aaaccagaaa agccgaacac
    12241 tggcagttct acgaatgaat cacagactca gccaacaact gaaccttcaa acaatcagca
    12301 agcaccgtct gttgctccaa acaatcaagc aacggcaaca agagaagaac agcaagctaa
    12361 accagcagaa agcgagtcaa aaccgtatca ccaaacgcca actgcgccta ttcaagaatt
    12421 agttccttca agcaaagaaa cggctgaagg aaacattcat tttgaaaaag atgagtccgt
    12481 agaaagcttt gtccgaaaaa tcggcgaatc tgctcgcaaa gtaggacagg agaacgactt
    12541 atacgcttct gtcatgattg cacaagccat tttggaatca gctagcggac aaagtcagtt
    12601 agcacaagcg ccaaattata acttgttcgg aatcaaagga acacataacg gaaaaggtgt
    12661 ttcttttgct acacaagagg atttaggtga cggcactctt tacaccacac aagctacttt
    12721 tagacagtat gaaaactatg aggatagctt aaatgactat gcacagctgc ttaaagaagg
    12781 tttgactggt aatagtcaat tttatagtgg tgtatggaaa acaaacgcaa aaacttatca
    12841 agaagcaacc aaattcttaa ctggcagata tgcaactgat acgaaatacg atcaaaaatt
    12901 aaatggtttg atcgagacct atcatttaac ggactacgac aaggaagtcg caggaccaca
    12961 attaagtaaa gaaggatata ttgtaccagt gaaaaactat acgatttcta gtccgtttgg
    13021 tactcgtggg ggagaatttc atagaggatt agatttggca gcagcacaag gcgagccaat
    13081 ctatgccagc aaagcaggaa cggtcgttaa agcggagttt cacccttcat gggggaatta
    13141 tgtagctatt gagcatgaag acggtacaac ggctttatat gcccaccaac aagaatatca
    13201 agtcaaagtt ggcgacaaag taaaacaagg tcagatcatt ggctatgttg gctcgacagg
    13261 aaatagcaca ggtagccatt tacattttga attatgttta gatcatagtt taagtcaatc
    13321 acaattggtt gatccagaaa cagtcttatt ttaaaaataa ggctgttttt tttattcaat
    13381 ttttaaggag gtattgtcta tgttgctatt tataagtggt ttatttttag gcggatttat
    13441 tggtgtggcg ttaatgtcat tagtagcaat cgatcgttat gaagatatga ttgatgaaag
    13501 aagataatta agaaagaggg agtacaaatt ttatgacgaa tagaaatgaa ggaatcacta
    13561 tctacttgac tattggtaaa gaagttaagt taaattttga ttcagagaat aacttatcaa
    13621 gtattcgtct atccattaat aaagttgcag aacttttcgg aaataaagta gtcaatgaag
    13681 aaagtatatt ttatgtttca acagtagcct tagtgacaaa aagtaactat aagactttta
    13741 tttcagtagt aggtggaaag caagttatta gtggagacgc agtttatgag gaagtaatga
    13801 ttgatcaaga aagaactagc aataaaagag gaaacaaaaa tgtatatgag caaggatact
    13861 ttgaattttt cgtgtcagaa gaagacaaaa ttcctaaaga gaaactaatt ttgccttaca
    13921 acgtaaaaaa acgtaggtaa aaagcttatg tgaggatata tctatgaata taacagtaaa
    13981 agaattaggc gaacctatag aactcgaaga ttatttagaa gaatataaat tccaagtcat
    14041 atttttatta aaaaatatga cttttattga tcgtaaagat aatcgggtaa gacaactttt
    14101 taatatggaa ctgattcgag cggaaactaa gtggtattct ttttaccaaa aaaatcaccg
    14161 tccacctgat tataactttc tacaagaaga gattacgtca tttggtgtca atcgtttgcc
    14221 gatgtttgat agaagaaatg agggacgtgt gaatgacgaa ttaagcgtag acaactttgt
    14281 tcactatcat atacaacaat taaagcttga aaaaatactg tccccagttt tttttgaaaa
    14341 aaatgattta cgattcatgg agagatacgc aaaaaataaa gcgaaagctt acttcaatga
    14401 gcgtgatcaa tatataaaag gctatgaaag ttttagaata tctcgaaata aaatcatgca
    14461 acgagcagga tatgaacaac ttaaatatga atttgtttct gataatatgg tccaaggtta
    14521 ttttaaagag gaggttgcta aaatggcagc aaatgacgaa cgagtagcgg aatttgtttt
    14581 tgatgaaggt aaaagtttga tgaaaagtgc aatacatcaa ctgaacagta taagtatatc
    14641 agctgctact ggtacaacaa aagcagtaac taatcagatt aaaaggcaga gaactacagg
    14701 aaaacagcgc tataatcaac tgaaaaagca tggatcgtta gaacatattg atgtctcaaa
    14761 gactgattta aagccacttg taaatgaact aaagaaatat aaggtagatt ttgcagtaaa
    14821 aagaaataaa gagactggaa aaatacatgt tttctttaaa gctcaagata gggatactct
    14881 caatttagca ttagaaaatg tgttacaaaa atttgctaat gaaaaagatc agaatagaga
    14941 acaacagcag gataaaccta ataaagatat tgaaaaatct tctgtcgatg acaaggataa
    15001 agataaatcg ttaactaaaa aacttgataa attagaggat aaagcgcaag aaataaatca
    15061 acaaagagaa caactaaaag agaacacgaa aaataaaact aaggggcaag aacgatgaac
    15121 atcaccaaaa aagatcttat ttttggtgtc gcacttgctg cttttttctt ttatttaggt
    15181 actttatttt ttatggttta tttctcgatt cctcaaaata cagattcttc atggataaat
    15241 gtagtaaatg aagcatttga taagttctta gaacctaaga catttaccat ttctccattt
    15301 actttttcaa aaattgctat gaaaggtggg gcgataattg cggttattgt tttactgatt
    15361 gatttatata ttataagtgg caagaaaaat ttaagaatgg gcgaagaaca tgggagtgcc
    15421 aaatggggta cttctaaaga cattgctcct tatgtagata aaaaatttaa aaataatctt
    15481 ttatttacaa atacagaaag aataacgatc gaaactaagt taaaaaagaa taaggaatat
    15541 gaacgtaata ataacgttgt agttgtaggt ggagctggtt caggtaaaac tagattttat
    15601 gtattgccca atttattaca aatgcactca agctatgtta ttaatgactc caaaggtgga
    15661 atcttgagtg atacagctgc cattatgcaa caaaatgggt atcggataaa agtatttaat
    15721 ctgaatacca tgaaaaattc tgatggttat aatcccttcc attatattag aagaacagaa
    15781 ggagattgtg aaattgatat attgaaagta gttaacaatc taatgaagaa tacttcagac
    15841 cctaacaaaa agggtggaga ttcattttgg gacgatgcac aaagatcttt tttggcagct
    15901 attttccatt tgttgatttt gaaaggaaaa cctgaagaac aaaatttagg gatagtagca
    15961 gacttagtaa gacgcgcaag agtaaaagaa gatgatgaag aatatatttc ttcgattgat
    16021 ttagtatttc aagaattaga gcaagaacag cctagttgtt ttgcagcaaa acagtatcag
    16081 aattttaaga tggcagcagg aaagacggct aagagtattt taattagttt aggtgttcgt
    16141 ttgtctcctt ttgacgttcc agatatcaga aaattaacgt caacggacac cttagaatta
    16201 gatacaatag gagatcgtaa aactgtactt tatattattt tgccagatac tcatactact
    16261 atgaactttc tagcaagtat gatttaccaa caactttttg atacgttagt ttataaagca
    16321 gataatgttt ataatggatc gttaccaatt cctgtaaggt gtttgttaga cgagtttgct
    16381 aatactggac aaattccaga ttttgaaaat gttattgcaa caacacgtag tagaggaatt
    16441 tcagttgatg ttatcttgca aaatttaaca cagattagta aaaagcttta taaagacagt
    16501 tgggaaacaa tcataggaaa ctgtgattct tttttgtatt taggaggaaa cgagcaaagt
    16561 actcataagt atatttcaac tcaattagga aaagaaacaa ttgatgtagt aacttataac
    16621 gaatcaaggg gaactacggg aagttttact aaaaactctc aaaagcaggg aagaaaccta
    16681 cttgatccaa atgaagtgcg tgagattaaa ggcggaaaat gtatttatat gttacgtggc
    16741 acaaagcctt ttttatctga tcgttttaag ttggaacgcc accctttatt taaaaagtta
    16801 aaagagacac cttcaggtta tatgtatcaa tcaccgttgg atcaagaaga atttattgca
    16861 aataatgatc caattgattt tgaattatca aaaaatgtcg atatgaagga gtttttgaaa
    16921 catacggaaa ttgaaactta tgattttgaa gacgaagaaa ttgaaacaat aaatttagat
    16981 gaattataaa aggagagaat aattaatggg aacatttaac gctattatag atattttgtc
    17041 caaatacgct attgtaggcg gaggcttata tgcagtatgg ggtgcagttg ttttaggtgg
    17101 tgcattaaaa gaccacaatg gtccacaaat gcaatcaggc atttggcaaa tcgttggtgg
    17161 agctttaatt gtagcagcag cggcgttatt taagacaatt gtttagagga tagtctttaa
    17221 tgaaaagggg atgaatgatg gacggatttt taacatggtg tgtcgaatct attttagaag
    17281 taatctcctt tagtagtaag acaagccact taactagcaa cttagaaaac tttagtcctc
    17341 agttatacaa atattcaaca actattcttg attctgtctc aaagccaata gctttcagca
    17401 tattggcttt gttttttgct ttagaattac aaagaatttc taatcgtgtg gagactaatg
    17461 gtggaggtca aactggtttt gaaatgattt ttaaatcctt agtgcgacta ggtctatgta
    17521 aactagcaat ggatcaagta tctttaatta tgcgagcgat tatggatatt acaggatata
    17581 tgactgataa gattaatcat ttaggtatgg gaaacaagga agtaagcgat atgctggacg
    17641 caaaaaaaat attagctccc attttaaaag aaggactaat gtttaaagta ggtgtatcga
    17701 ctgttttaat tatcgcatta ctaattacta ttggttcgat agtaatcgta caggtagttg
    17761 taaatatgcg ttttattgaa ctatacgcat tcttagctat gtctccaatt ccaattgcga
    17821 catttcctaa tgatgaatgg tcgagtatag gtaaaaactt tctaaaatca tttacagcaa
    17881 cagcgttaca gggaacttta atatttatgg ttatgcagtt ttatccatat ctcttaagtt
    17941 cggctttcag tgatataaca aatagtggtt cagcaagtga aacattctta atgacactga
    18001 ttgggataat tggtaatagt gtgttactaa ttttcgcagt aatggcgact ggaagatggt
    18061 ctaaatccat tacaaatgca atgtaggaag gtcttttcaa tgaaaaaatc tcgttgtaac
    18121 agcactctaa tttttggagt gctatttgtt tttttgcaaa tactatccat atcattaaca
    18181 acttatgata tttcaagtcc attaacacta agtatacttt atgtattttt tattgtttct
    18241 cttgttttta cactcataat gatcgtaatt attacaaaag agatgttgcg agattttaag
    18301 aaattaatat ctgatatttt ttcacagaag aaagaaaagt aggtgtttga gtggcaatta
    18361 gagtaaaagt accaaaagaa attaaggatt atcaagaaaa attaatggga ggaatgagcg
    18421 ttaggcagtt agtatttttt gtattggcag tattaatctc agtaggagta acagcatatt
    18481 gtgtattggt attgcatatg tcggttgacg cagccggtta tatagttatc ccactattga
    18541 tacctattgt agcatttggc tggataagaa aagaaggaat gcctttagaa aaatatggca
    18601 agattattct taattattac tcgaaaactg gaataagaat atatcagact aaagaaagga
    18661 gcaatctaaa tgattttaga aaaaaacgat tccaaaaagt caaagaaaca accaaaagat
    18721 aagcaagcaa aaaaagtatt aaaaatctta gcaaagcaga aaaaacagcg tgggaaaaat
    18781 caacgtagag acgctccgcc tttatctagc caacgatcct taacatataa agcaatgtat
    18841 gagaatggag tgtgtgagct taaagaaggg cttttttctc gtacattcaa attttcagat
    18901 atcaattatg caattgcaga tcaatctgag caagaattaa cttttagctc ctattgtgag
    18961 ttactaaata gttgtgattc tgatacttct ttgcagattt caattattaa tagaagtaaa
    19021 gacaagaaac aatttgaaaa agacttatct tacgaattag ttgatgatgg attagatgta
    19081 tatagaaaag aaatgaatca tatgatatct aataagatta aatatgggaa tagtagttta
    19141 caaaaggaaa aatatattac ttttaatcaa agagcaaaaa ctttagaaca agctatacca
    19201 gctttaaata gacttcaagg cgatttagaa gatcaattta gcgaaatggg gtgtgaagta
    19261 gaagaactgt caggaattga gcgcctaagg gttgtcaatg atattctacg ccccaataaa
    19321 aaacttcatt ttgaatatga agatttgctt ttcagtggtt taactacaca ttcagcaatt
    19381 gcgccagata gttttgattt taaatatcgt aaggatcgtt tcaagatggg agattattac
    19441 ggtcaagttt tgtatcttcg taattatcct gctgaacttt cagataaatt aattactaat
    19501 atcacagata ttcctactaa tttacagatt actttgcata ttgagcctct agatcaacaa
    19561 aaaggtttag atttggtaaa aacaaaatta tcatttatgg agcaacaaaa gattgatgag
    19621 cagaaaaaag cggctagaag taattatgat cctaacttga tacctatgga attaagaaga
    19681 agtatggacg aagcacaaga attgttggat caaatgcaag gagacaatga aaagctcttt
    19741 tcttttactt ttttagtgat ggcggttgat aaagatgaaa gaaagcttga tgacacaatt
    19801 gagcaaattt taactgcagg tcggaaaaat gattgtgagt ttgcaaagtt aaattatctt
    19861 caagaagaag gtctaaactc tacgctttta tggggaaaca attttgtccc tataaatcga
    19921 acattaacaa ctgctagtac tgctgtattt attcctttta ctgcacaaga acttatgcag
    19981 ccgaacggaa aatactatgg tttaaactca gtaactaaaa acttagtgac attagacaga
    20041 agaaagctaa aagctcctaa tggttttatt ttaggtactc ctggtagtgg aaaatctttt
    20101 gctactaaaa aggaaatgac agctgtttta ctgagtgatc ccgatgctga agtaatagta
    20161 attgatcctg aaagagaata taccccttta gcagaagctt taaatggaga agttattcag
    20221 atatccgcag gatcaaaaaa tcatgtgaat ccgatggaaa ttaataatga ctacgcagat
    20281 gatgatgatc cagtcatttt aaagactgac tttgtgttat ctctttgcga aatgttagtg
    20341 gggggaaata ttggtcttag tccaactcaa aaaactttaa ttgatcgttc atgccgattt
    20401 gcctacaaga aatattttga aaatgggtct ggaatgccta ctttaaaaga tttttctaat
    20461 attcttaagg atcagcctga agaagaagca agacaattat cagtagagct tgaactttat
    20521 actgaaggat cattatcggt gttttcccac caaaccaatg ttgatacgag caaacgtttt
    20581 atcgtatttg atgtaaaaga tttaggaaaa caattgcgat cgatgggcat gctgattatt
    20641 ctagatcaaa tttggaatcg tatcacaagt aatcgaaatg caggacgtcg gacatgggta
    20701 tatattgacg aaattcagct tcttttttca aatgaatacg cagagaatta cttttttgaa
    20761 ttatggtcaa gagcaagaaa atggggggca attcctacag gtatcaccca gaatgtgtct
    20821 acgcttcttt cttcattaaa tgcagaacgt atgctatcaa atagtgattt tgttctaatg
    20881 ttaaatcaat cccaacgtga tcgagaaaga ctaggcgaat tgttacacat ttctagccgt
    20941 cagaaaaaat atattacgaa tgccaaagaa ggtgcaggat tgatgtatgc gggcgattct
    21001 atgattccac tttatgatga gtttccaaaa gatactaact tatataaagt gatgtcaact
    21061 aagattgagg acgtgtataa gtatcaaaat gagcaaagag aacaaggtac tatagtatga
    21121 acacagattt tcgagaacgg attatccgaa aacaaaagac agcaaaaaag tttgtagtaa
    21181 atcgagataa taatcagttt aagaaaaaaa ttgtatctac tgaccataca aaacgagcta
    21241 agaaagctaa tcatagtgga aaaataaaaa gtagtcgggg cacttcctat ttaataaaaa
    21301 aagattcagc aaaaaatgta gtcaaagatg ttaactctaa agcacaaaaa aatggtgttg
    21361 aacaggggaa tgcaactgct gaattagaaa cgagtaaaac aataggacgt ggaataaaaa
    21421 atagcactaa ggcaacaaaa acagtggcta aaactacaac taaagttgct aaaaaaagta
    21481 aagaattgat tcaaacaaat gcttcaaaaa aagaattttt aactgttgtt aaaaaaggaa
    21541 gtaaaaaagg tctcgtaaac acaagcaaag ctgcaaaaga tacagtaata aatgaaatcc
    21601 aagagtttag aggcgactca gaatctcaag ggattaatga agcaattcaa ttaaaagatt
    21661 tcattgatac aggtatcaaa ggtgctaaaa aaacaaaaaa agcagcaaat actgctaaaa
    21721 aaagtagtaa agcactaaca aaatttggta aatcaagtta caaatataca caaagaattg
    21781 ctgaaatagg tatacgtaga gttcaacaag ttttatcaaa agaagtagca aaagcactta
    21841 ttgctaaatt agcgattcca ctaactttag cagtgcttat attatcaatt gttaccgctg
    21901 taatttcaac tgttaacatt tttaattttg gagcaggcga ctgtgatact agtgctaact
    21961 atgacaatat aactaccaaa gatatagagt caaatgctaa aagtatttac tcaattattc
    22021 gtaaagaaat acctgagtcc acccctcaag ggatttctgg agcattagga gctatgcaat
    22081 ttgaatctca attgaatcca agtgcagtta atccaagctc aggtgcgaca ggaattgccc
    22141 agtggttagg ttctcgttta acctctttaa aagaatttgc acacaaaaaa ggaaagaagg
    22201 aaacagattt aggtgttcaa gtagaatttc tgttatctga attaaatagt tcatattatc
    22261 aatctagcaa aaaaatatta gcaatgacag atgttcatga agcatgcaaa gagtgggtca
    22321 tgaagtttga aggattatct caagactcta gccaatggta tttggatcaa agaaactctt
    22381 atgctgatca ttggtacgca actttaggga cgagtgatcc aattgccgaa ggtaccatta
    22441 gtaatggtgc agatagcgaa ttaaatacgc ttacttgtga tttagaatct gatggtggcg
    22501 atattatgga aattgctaag agttggctag gttggtttca ctatgatcag atacaccctg
    22561 ctcctgattt aggaacagat ttaaaaaatc ctaataaaaa tggaagaact gattgctctg
    22621 gttttgtttg gctagtttta aataaagctg ggtataaagt acctgcaaat atgcaatggt
    22681 ttactggatc aatggcaagt gacgctaaag gatcacacca atatttgaag caaatttcag
    22741 agacagaagc aaaagcaggc gatattgtta ttgttaatca aggtgctgga gcaggtagta
    22801 atggacatac aggaattttg actgaaaagt ggagaggtaa agataccaaa atcattcaag
    22861 aaggtggaaa cggcgatagt gtaaatattg aagcttttgg tacgtccttt atgagcttat
    22921 tagatggtgg cgatgttgta ttagcacgtc ctataaaaaa ataattagag gggaggaagt
    22981 actattgctt aatagaaaat cagttacttt aattgtattg gtattaattg aagcgatctt
    23041 ttgtttctcc attgggttaa gtattaatca agaattaact gtaaattcta aaataacagc
    23101 tttagaaaag aaaaatgatg ccttaaaaag agaaacgaaa gaccaaaaga gccgaattag
    23161 caaacttgaa gatgaaaata ataaaatgct tgaaacacaa gtagtaaata aagatggcga
    23221 tgtctacaaa gaatttacaa acattgctac taaatacttt aaatcaagat ttaattatga
    23281 tcccaaaaca tacaaagaaa gtaaggaaaa cgtaagagat ttgatttctg aagagttgta
    23341 taagaaattt tcggagaatc agctaactta tggagattct aataatgtga gttctcgatt
    23401 agataattta gaaatatata gtggttcatt aattagccaa aaaactatta ctggtctttt
    23461 gattgttgac tatgaaagta aattaggaga aactgactgg tttaaaaatt cagaaatcta
    23521 tactgttcag tatgatattc atgcgaagaa attgactaaa atacaaagct tggggtcagt
    23581 tttaagggga gatatgattg aataaaaaaa ttttttctat ttgtgtctta atttttactg
    23641 tgtctatcat atttttagca gatcattctt atatcaaata tatagaagca caaaatgatc
    23701 cagtctattt agatgaatca aatcaagaga aaatagtttt ctacagaaac gattgtagag
    23761 actgtgaaaa gatcatgcct tacctgatac gtcacaattt tttataccat gacttaactt
    23821 ttgttaacat gaataacaaa gaaaataaac gttatattaa ggagtatggt ttgacttccg
    23881 ttcctacaat catttccctt aagcagcgtt atgtaggtac agatataagt aaaataaaaa
    23941 cattagttat gtcaaaagga gatgatttct gatggcaaga aagagcttaa cagaacgtat
    24001 tcagcagaag aaagaagaag caacaaagct tgaaaagagg attcaagaag atcaatataa
    24061 gctagaaaaa ctaacaaagc aaattagaga tttagaagca gaacaggttt tagcgattct
    24121 tgataaccat aagcttagtg tgaaagattt gaataaaata ctaagtaaac agcctgataa
    24181 caataatttt atcaaccata attaattaaa tatatatctt tttaattgag ataataagcg
    24241 atataataag ataaacaagt aataagaggg tgaaagtatg ttaatagcta aaagaatatt
    24301 agcaagtata gtagcaattc cttgcttttt actttccata attgttattg ccgttaacat
    24361 aatttccata cctattttgc ttgttataca gattcttaaa aaagttgtga tcctttttat
    24421 tacattagta tggattggtt ttataggtct gtttatttat ggattaagtt ctttaggctt
    24481 ctctgaaacg cttagtatgt ttacagtgcc tactataggt atattaatcc ttacattaat
    24541 actatctatt tttactttta tctcagataa aattcctgat tttcttggaa ttgttagttc
    24601 atttttcctt tctatcccaa aatttctatg gggggctgaa gaaactgaag aagctgaaga
    24661 ataaaacatt caagggggaa aaatatgttt tcaaagaaaa aatatgttca gtttatagaa
    24721 caaagagata ttgaattgcc agaaacccct gtagaaattt acaggggtat ttatttactt
    24781 gaacaggaaa acgatcggct aacagacaag catactaaag gttctacatt aatattatta
    24841 accgataaaa agggaaatga actaaataga cgactaatcc aattaccaat agaacaaggt
    24901 atagagacta ttttgactaa ccttttaatt gaacctacct caaataaaaa gcatagttct
    24961 tctaagtcac aaatgatatt gaatgatata gaagaaccaa aaataaagaa taaaaagaag
    25021 accgtaaatt atcagacggt cttcttttta tttactacga atttagcaaa aaaaatctac
    25081 atggctttga taatagttct gttcctattt ttcacaagtt tttttgtatt aaataatcat
    25141 tcatcaactt ctactgattc tcaaagctat catgagcttt taaagaagaa agattatgaa
    25201 aaaataataa aacaatatcc tgaaaaagaa aacgaattag tagaaaaact gtatagcgaa
    25261 aaaaattcta aggaattaaa agaattggca gagaaatctg attcacagat agctcttttt
    25321 tattgggatt tcttgaatga gcattggcaa gcagtaacta ctatgaaaaa tgttacacca
    25381 aacgaaacaa tacaagctat gcgtggttat gcatacttaa aacaaggaaa actagacgaa
    25441 gcagaactaa ttaacaaaga aattgataat caaacattaa aagatcaaat ttatcaattt
    25501 aaaaagcaag aagcatataa agctctgcat gaaaaagata ccgagaaggc agagaaaata
    25561 aataaagaaa taaatgattc tgaactatcc gaagatatta aagtagctaa aagtatggtt
    25621 aatctacttc aaaaatatga aaaggacaga tcggattcaa aattatcaga agatgaaaga
    25681 aaagaagctg agaacaatta caaaatgtgg tcagaaaact taaaacaact aggaggaaac
    25741 gataatgcat aacaaaaaga aatttttagt agtgggacta ttttcagctg ttttattagg
    25801 gggtgcagtt acttatggca tgtattctca acaaccacac aatgagactg aaaagacaga
    25861 acaaacaact aaatcagagt ccagcaaaca caaaagtaag aaatcagcat gggaagaaag
    25921 tgtgaaaggc aaagaagaca aaaaggacaa aaacaaacat tctacaaaga aagctgatga
    25981 tatattggct gttattgctg atacaccgct aattaaacaa caacctaatc aaaacaaaaa
    26041 taatctaatt gatagattag cagtagcttt agatgatcag actaaaaaag aggcagacag
    26101 acaagaaaaa ttaattatta aagataaaaa atctgtagat gagccagtac tagttgattt
    26161 gagttataaa cctcaacttg aggatactaa accttcaaat aaagagaacg gtaaaacaga
    26221 aaataataaa gatgaaaata gcgaatcagg taaaggaaat gatactaaac ctaataattc
    26281 attacccgat ccattgcctg taccagagcc aaatccattg cctgatccaa cacctagtcc
    26341 agacccaaat ccaataccaa caccagatcc agatccagac cctgatccag agcctgagcc
    26401 aacaccagat ccagaacctt caattgatga acaaattaat caatccaagg aagaactcaa
    26461 tgattctagg aaacaggctg atgaattaaa taaacaacta actcaagtaa aagatagctt
    26521 ggataactta caaagtattg gagcaagtac tgaaaagaac gtggacaaag cagcattaca
    26581 atggcaaaaa gttgctgact tagtcactga atataatcaa cttacaaatg agatcaagaa
    26641 attaattgaa gaagacggaa cagtgttgcc aatcaattat gatctttata aagaaacgta
    26701 tgaaaaacta aaccaaaaag tcactgaagt aaaaactgca caaaaagaag caactaatac
    26761 gacaaacgaa ataaataaaa atgtagaaca agcaactgat acgctaaata atttagaaga
    26821 aactaaaaaa gaatatcaaa ataaacagaa aaatgtttct gaggtggatt ctaacgtcaa
    26881 ttcggctatt aaccatgcac aatccaatga acaagttgct tcagctgttc aagatgagat
    26941 aaatcaagct gcacaatcta atgaacaact aaatgaaact aataagactg ttggagaaca
    27001 gttgaatcaa gttgatccaa ttgaaagcca aactgatatt gctagcgcta aggagcaaac
    27061 tgctgatatc aataatcaag ctgaagaaca aaataataat gttgacgata tgatgaatga
    27121 ttttaacaat ttgccaaaac cgagtgatag tgaagaacct agcgttccag aaaatacaga
    27181 tcaaaatcaa gattcaacaa gccaaccaca aaacatagat aatgctagtg tttctcaaaa
    27241 caaaatagaa aatgaacagg ttagcgaaaa aacattacca gttactaatc aaaatagtaa
    27301 ccaatccgaa attgaagata ttcaaaaata aaagtgagga atgaaataaa tgtcttataa
    27361 agatagtgaa gcaaaaaaaa ataataaaac cgctgaatta ctttcacagg cagaagaagg
    27421 tattaaagaa gtattatctt cagacaaata caaaaatttt cttcgtgtta tgtctaagtt
    27481 tcataattac agcttaaata ataccctttt aattcttatg caaaatccta atgctactca
    27541 tgttgctggt tacaaaacat ggaaaaactc ttttaataga caagtaaata aaggagaaaa
    27601 atccattaaa ataattggcg gtcgtcccta taagaggaaa gtagaagaaa taaagaatgg
    27661 gcaaaaagaa gaaaaagaag ttgaaggtat ttcatttttc cctgtttcag tttttgatgt
    27721 ttctcaaact tcaggagaac ctttaccgca actaatgaat gaattaagtg gagaagtaaa
    27781 aaattatcca gagctttatg ttgccttgaa gcaaagcacc aatttttcta tagaatttgc
    27841 agatatacaa ggtggcacaa aaggatattg ttctccatta gaaaagaaaa ttgtgttaaa
    27901 taatggtatg agtcaagcgc aaacaataaa aactctgatt catgagatca ctcatgctga
    27961 tttacatgca ccagaatttg ataaaaattc gtcaataaaa actacaaaat ctacaaaaga
    28021 agttgaagct gaaagtacgg cttttgttgt ttgtgaacat tatggaatag atacaaaaga
    28081 ctatagcttt ccatatttag cagcttggtc tagtgataaa gagttaaagg aattgaaaaa
    28141 tagctttgaa gtcatattaa agcaagcaga taatttaatt caaaaaatag acaagaattt
    28201 agcagaatta caaaaagaag tgacaaaaga aaaagaagaa aaacagagta ctttgtcatt
    28261 atctgatcaa ttagaagaat ttgaagataa agcaaaatct ttaaatgaac aacgtgacaa
    28321 agaaaaatta attaataaaa ttcgccaaac aaaagagcaa aaagacgttt ctacactata
    28381 gtttaaagta gctgaaacga tctattttgc ttttttaatt tatcagaata agtaactttt
    28441 ataataaatg agggatataa aaatgaaaca atatttagat caatagaata taattgaagg
    28501 agcattaact gtagaaagaa ttaatgaaat acctgattgt ctagaaaaag atcatttatt
    28561 tttaattaga aaaatgttaa aagaagagaa ttttgaaccc aatgatttta ttgttgttga
    28621 atatccagct actggaatat actgctgtaa tcatattaac aacggtcaat attttattat
    28681 tcaagaagaa aaaaataaat tagttccata ctatactact tgtgatatga atgaagaggg
    28741 aattaataat tttccttgta cgagtatcaa agaatccatt aaattaatag aaatgtaaaa
    28801 gagagggaca tataaatcat gcaaacattt actcaacacg aattagaaat tattagcgga
    28861 cacaaacaga ttgcaggttc aaatgcaaat aaattaaacg tcataaaaag cgaagttgga
    28921 gaaatgcttt caatgttaaa aagtgatcta ggaaaacaat atattgaaga tgatgaacaa
    28981 cgacattaca cattaaagaa ttatcagtct gtttatgata aactcgaaaa aatgagtgaa
    29041 gatgaattta aacaaattga tctgagcggt attgaagaat tggaatagaa acggggattg
    29101 gggcagcgcc ccaaaatgca aaaagaagaa gtcgcgtgag cggctttttc ttttttttcc
    29161 gcaagttggt accaacttgc aaatcttgct gtaagaccgc ccccctgaaa tcttcgattt
    29221 ctaatcggag ggcggtcaca cagtggaaat tttcgttttt aaacaaagat aaaacactaa
    29281 actagtgaaa aaaacaaaaa atattggtaa ggaggggaaa atatggagca aaaactagct
    29341 aaaactcctg aaaatattct acaaatcgct aaaacttacg acaacgatga aagaaatatt
    29401 caaaaaaaat ttagagtatc tgaaagagaa aaattcttaa tagactacaa aatgagtcaa
    29461 gcaaatacaa gaaatttttc ccgatacgct cgtattatgt gtttaagtgg tttaatgatt
    29521 acaacagatt tttctgattt aaaaaattta actacagaat taggacgaat tggaaataat
    29581 atcaatcaat tagctcacag agctaatatt tcaaacagtg tttcaaaaga agacttttta
    29641 gaattaaaaa taaattatga tcaattgact aaattaatta acgaagaatt actttttaga
    29701 atgcgacgtg ctagagataa tcagaggttt atttaatggc agtgacaaaa atacaccaaa
    29761 taaaaactac tttaaataaa gcaattggct atattacaaa tccagaaaaa acaaataatg
    29821 gaatgctggt tagtagctat aattgtcaaa gtcaatttgc agaagttgaa atgcaaatga
    29881 ctagggaata tgcacgtgaa gtaaaaggcg attatcgaaa agtcggtggg agtgaggttt
    29941 tagcgcacca cttaatccaa agtttttcgc ctgaagacaa agtgacaccc gagcttgctc
    30001 atgaattagg acgacaattg attgatgaat tgactgaagg aaagtttgaa tatgtgattg
    30061 ccactcacat agatcaagaa catattcata atcacattat ttttaacagt gtttcttttt
    30121 atgatctgaa aaaattcaga agtcaacctt atcgaacagc aaataaaatt aaagaaatta
    30181 gtaatcgtat ttgtgctgaa catgatttat ctttatcacc gaaaaaagaa aaattaaaag
    30241 attcctacaa atcttattct aacagaagaa ataacacgag ctttcgttct gaaattcgta
    30301 aacgattgac catgtgctta aatgaatcaa cttcatggga ggaatttgaa gaaaaagcgg
    30361 atcatttagg ggtaaatatt gatcgaaaag ggaaacatat ttcgtatgct ttattatccg
    30421 aaggacaaca aagacggact agaggtgaca agttagatga cttagaaaca tttacagaaa
    30481 gtaatatttc ccaaaaactt tctgctaatc aatcctacat taatcagttg aaagaagcga
    30541 ttgaagagac attcttttct agtgacaact tggaagattt tagttttctt ttggaacaag
    30601 agcatggaat tacctttaaa tcaaataaac aagatcaatt tgttttccat ttcaatgatg
    30661 taaattattt taaatggaat gaaagcttgc tgccagatag ttatcagatt aaaaatctaa
    30721 aagattattt tagagaatcc tatgagttta cgaatgactc ttccttaaat tcaattgaag
    30781 aaagatatcg tgaaaaagaa aaaagtgtga ttgttcaaga agatacgcct attcaattgc
    30841 aagaatttca gatagagaaa gcgaccaaag aaggattgct agtgtctatc gaacatgaag
    30901 acttaaatgg tttagtgttt gtcccaaata attatgttga tgtgaatgaa gaaactggag
    30961 aatatacaat ttggatcaat gacaaatttg attatacgct tactgacaaa cagacaaacg
    31021 aacaaactag ttttactgta aaaggggaag ggatcattcg tggactggaa aaaatgcaag
    31081 ggattcaacc acaagagata aaactttcta gcaaaaatat tttatctatg agtaatcgtg
    31141 gtgtatctat ttcgatacca gaactgaata tcaagcggct ctttattcct agtgagtatg
    31201 ttgtaatcga tgaaattagt aaatcttgtt ctatcttaat tggagaaaag tggaattact
    31261 atgcagaacc actccctgag aacgatgata attcaaataa aaaattacct tatcaaaaat
    31321 ttactggacg ttctttaatc agtagtttaa aagaaactcc tgaattactt gatgtgtttc
    31381 taaaacctaa atttgatcag ctacattatc aatcaagaaa acgatatact aatcaattag
    31441 tcgaagcttt aaatacgatt cgtgaagaaa aaattgtttc agtttcacaa ttagagcaga
    31501 gattgaatga aattgttgag caaaaaaacc aagcaaaaga aaaaatagct gagattgatg
    31561 gaaaaataaa aaattataac aatgtcgcaa aacttttagt aacttacgaa aaatatttac
    31621 cgatcatgaa tgaaattgag cgtgctacaa tgcttcaaaa aattaaatta gaacaaaagt
    31681 acaaagaaga agtacgccaa tttaaatttg ctgaaaagaa attactagct tcagataatc
    31741 tacgtcctga tttaacgaaa gaaaagataa tatctgtggc gaaaaatcaa gagaaacaga
    31801 ggaaacgact tatagagggc tataagcatt atgaaaataa attaaataga ttgattgatg
    31861 ttcaggaaat attgcaagaa ctagatagaa atggattatt tgatcttagt gggtatcgtt
    31921 tgattgatag agaagaaaag ctaacggaca agatcgagaa gtatgaaaaa gaagtagaag
    31981 aaactaaaca aaaaaatgca aaagagaaga ggaaggaaca cgaaatataa ataactaaaa
    32041 acactatata ttccgaaaag tgagtatctc cattcataaa ataatcaaca tatttaaaaa
    32101 atctaattag cttttttagt caatctaaag ttctttattc tatgatataa tgttactgta
    32161 gaaagtttgt ggcggtttgg caaatccaat tgaaggagcg tggtgcctat gggcttatta
    32221 gcttatcgga cgtactctgt agaaaggaaa tgcctgattt gttctcattg gatcaaatca
    32281 ttgctattat cggagcagca gcagtagtta ttactgccgt agcgaaagct agcgattcga
    32341 ttttagcttg gttaaagttc aaaaaagaac aaaaaaaata gagccgtctc aacttttggc
    32401 gaggtagacg agctctaaag ctttatttac atgccatccg ctttgaacgg actacactgg
    32461 gcatgtgaca gcatgccctt tcatttatat tatatctcat tcttttatat aatgaaagta
    32521 aataaatcat ggaggataaa atatggataa atttaataga ttaacattaa taaatcagtt
    32581 tgaaatttta aaagctttaa atcctaacga agaaaaatac tatgctgaaa aaatagaaat
    32641 actaacagaa ggttacgaat atcactatag tgaaatattt gaaaatattt ctgatccatt
    32701 gccagaagaa gattcgcggt ttgttctaga catattaaat atgtatagag atattacttt
    32761 tagtaaaaat aaattaaaag atataaattc aattgataat tattatatac aatttaaagg
    32821 ttttgacttt aatagctcta ccgaatttaa gttagcactt tatgcacaat tttttattga
    32881 aaaattaaat aggtttcaag aaatagttga agatagtgat tttaacactt ttaatagcca
    32941 tacaccgatg agaggtactt atataaaatt tctcgaaaat tataatgatt taaaatctac
    33001 caataattat caatttggaa acttgtctta tgagatgata agtaaagttc taagtttgtg
    33061 atgataaatg aaattaaata gtataaatga tttatttgct attataggtg ttattgctaa
    33121 catacttttt tcgtgtttga attttagatt tactcaaaag aacaaaaaga acaaactcag
    33181 tatagaacaa atgaaaaaag acattgaaac tgatttaaaa aatgaccata aagaaagaag
    33241 aaaaattgaa gaatatactt ctttaatgga aaagtgtaaa gcaactgatg caatttttag
    33301 ctttttaaga aaaagtgatg agataatcac acgtgaaaat atagatttag aaaatgagat
    33361 tagtaaactt gaatctatct tttccgatat aaattctctt aagtatttaa tcgtcgaaga
    33421 aaattcggat agctttttag aatttgaaaa atatctaaca gatcttatag ataatagaaa
    33481 taatgaatta atgagacaaa acattgcaag attccttact aaattagttt ctgaatggaa
    33541 aagtattatt tctcaaaata tggataaatt aagatattaa ggatgttagt tttaaaataa
    33601 actaatgtcc ttatttttgt gttcacattt ctgttaccta agaaatactt aaaggaggtg
    33661 atgtagccag caacgactac aacttgaacc cgctatctac agtttatttg ttgtttcttt
    33721 tgggtatact ctaatggtac aaatccatat ttgcttaaag cgttgatttt tcagcgtttt
    33781 ttctttattg taaagtgtac cctaagacct gtatactaat agtaaataga ttggatagga
    33841 atgatggcaa atgtatttag cttatttaag agtttcttcc gacgatcaaa gtttagctcg
    33901 gcaacataag ctaattgaaa actggtcagt caaaaataat attgctgaag acgatttaaa
    33961 aattttcgag gaaaaagtct ctggaaaaaa tattgaaaac agaccagagc tgcaagagct
    34021 tatttctttc atacgtgaac gagatacgat tattattcct agtcttgatc gattagcaag
    34081 aaatagtaaa gatacaaaag acttacttca attgtttcgt agtaaaggtg cttctattga
    34141 aatattagac ctaccgtcgt ttaatggggt aactgaccct gcattaagag acttgctaac
    34201 aaatttagtc atagaagtgt ttagctatgt tgctgaaaac gaacggaaaa aaatcaaaga
    34261 gcgtcaaaaa caaggcattg aaattgcaaa gaaaaatggt cgttatactg gtaaaaagat
    34321 tcaataccat gccaatgcga caggaaaaaa caaattgatt tacaatgaaa ttgttgatgg
    34381 tctgaagaaa aaagaaacta tttctaaaat agcaaagaat gcaggggtta caagaaaaac
    34441 tgtttatcga gtaaaaagag atttaggaat gtaataagtt ctagttagaa agaagcgtaa
    34501 ataatgcaag aaatagaaat cggtaaaatt tattattgcg aagttcctag tttggaaggt
    34561 tcttttgaag ttcaagtttt acaaatgcta aaagacaact gtgttttagt gagagtcgta
    34621 cgttgtacat taatgcaaag taaatattta aagaaaagca aatatcgttt tattgtcaat
    34681 atcaagtccc taaaaggacg atcgttacac ttttaataaa gagatggagg actaaataaa
    34741 aatgaagaat aaactgacat tattaatttg tgcaaccatt ttgtcaacgt gggcagtccc
    34801 tacacaaaat gcttttgcag aagaaacagg gaatgatact acgcagcaaa cagtgactag
    34861 caatagttct agcgaagagc aaacagcaat tataaatgga gaagaagtaa aattagaagc
    34921 gccagtgaaa gaaggacagt ctgaagctga agcgactcaa gcaccaaaag aagagttaca
    34981 acaagatcaa atgagtcggt ttgctcgtag tgcagtaaga acaataccta tgtatcgcat
    35041 gtataatcca aatagcggag aacacttcta cactcaagat actggagaac gagataatct
    35101 aagaagaatt ggttggaatt acgaaggtat tggttggcaa gctccaacaa gtggcgatcc
    35161 agtttatcgc ttatataatc gctataatgg ggaacatttc tatactttaa atgccaaaga
    35221 acgtgataat atcacaaaac aagggtggac gtatgaagga attggctggt actcttataa
    35281 aggtgctaat ggtattccag ttagtcgctt atataataaa cgagtaaatt ggcaccacta
    35341 cacactagat gaacatgaga aaaacgtgat tagtaagcaa ggttggaatt atgaaggcgt
    35401 aggttggtac gcagtcggca atggacaacc aagcaatgac gactataagc ttcttggcgt
    35461 aaagaattac aatcaatatg ctttaggcgc tccaagtggc tgtgaagggg cttcattgct
    35521 acaaggatta cagtataaag gaaagatcac aaattggagt ttaactcaat ttttaaacac
    35581 gattcctaaa tctccaaatg gtaatccaaa caatgggttt gttggtagtc cattcgttga
    35641 aaattcttgg acttactcag ctatttatcc tgctccatta gcaacttggg ggcaaaaata
    35701 tggaaatgtg cagaatattt caggtagttc tatgaataca ttgcttaatg aagtaaagaa
    35761 cggaaatcct gtggttgcat gggtaactat caatttccaa ccaattcgtt ggggaaattg
    35821 gagttttggt gtagcagcta ataataatca cgcagtgaca cttgatggat acaataaagg
    35881 aagtaatcaa gtacatgtat ctgatccaat tagtggttct tattggctga atcgaacaac
    35941 atttgaaaat atttacaacg cacgaaaata tgcagttgta gtcagataac attgtatgct
    36001 tcaagatcat ttgttaatat taaggaacgg aactcccaaa aattccgtac tctaatttgc
    36061 agtctctcac tcatttagag tgagagtttt tgttaaattt tcaaattatt taataaagtg
    36121 atacaatgag ttgatcatac aaatcaaaag aagaagcagg gtaagagttt acctacgctt
    36181 ctttttttgt gtgtttagct atttcttctt tacaatatcg aacgtacgtt tgtataatgt
    36241 aaaaagaagg ggtgttattc atgtttgatt attcgaaaga accagtaaat gattattttc
    36301 taatcgacat gaagtctttt tatgcgagtg tcgaatgtat agaaagaaat ttagatccat
    36361 taacaacaga acttgttgtt atgagtcgag ctgacaatac tggttcagga ttgatattag
    36421 cttcttctcc tgaagcaaaa aagcggtatg gtattacaaa tgtgagtaga ccacgtgatt
    36481 taccacaacc atttcctaaa acactacacg ttgttccacc acgtatgaaa ctatatatca
    36541 agcgaaatat gcaggtaaat aatattttca gaagatatgt ggctgatgaa gatctactga
    36601 tttactcgat cgatgaatca atcttaaaag tgacccgatc actgaatctt tttacgactg
    36661 aaggaacacg aagtcagcga aggaagaagc tcgctcaaat gatccaagaa cgtattaaag
    36721 aagagctagg attgattgct gcagtaggtg tcggagataa tcccttgtta gccaaacttg
    36781 ccttagacaa cgaagcgaag aacaatgaag gatttattgc cgaatggacg tatcaaaatg
    36841 ttcctgataa agtatggaat attcctgaaa tgacagactt ttggggtatc ggttctcgaa
    36901 tgaaaaaaag gctcaaccaa atggggatca tgagtatcaa agatttagct aattggaatc
    36961 catacaccat taagagccgt ttaggcgtga ttgggttaca gctgtacttc catgcaaatg
    37021 gtattgatcg aactgacata gcaatcccac cagaaccgtc aaaggaaaaa tcttatggca
    37081 attcacaagt gttaccacga gattatacta gaagaaatga aatcgaactg gtcgtaaaag
    37141 aaatggctga gcaagtagca atccgtatta ggcaacacca ctgtaaaaca ggttgtgttc
    37201 atttacacat tggaacgtcg atcttagaaa cgagaccagg gttttcacac cagatgaaga
    37261 tacctatcac agataataca agagagctac aagactattg tctgttctta tttaataaat
    37321 actatgaggg gcaagaagtc cgacacgtcg gaataaccta tagcaagcta gtatacactg
    37381 attcgcttca gttagatttg tttagtgatc cgcaaaaaca gattgatgac gaaaaactag
    37441 ataaaataat agataaaatt agacaaaaat atggttttac gtcaattata cacgcttctt
    37501 ctaagcttga aggtgctaga agtattacaa gaagcacgtt agttggtggt catgcaggcg
    37561 gcgtaggagg gcttaaaaat gattgattac aatgatcgtg gacgtttaaa gtgggcagga
    37621 ttctatttat cagatcatac tgaagaaatc gatgctgaga atgaacagcg agccaatcaa
    37681 aacttagcaa aagaacaaat gacgactgaa gagattacag aagtattgaa cgctgcagtc
    37741 ataaaaaata gatcagtatg tattcaaaaa gaagaacgaa atgctgaagg atactatcca
    37801 gatgatatta tcggtaaaat ttttggaagt gatgagctag gaatctatgt cggaaaaaac
    37861 aaagttcact acgatgaaat acgtcatatt gaattttacc agaaacataa atggagctgt
    37921 ttagaatgaa cattataaag caatatgagc ttaagtatat aacacttgaa aatctttcag
    37981 atgaaatctg gggatatggt caacaactaa ttaatgaaat aggaatcgac tgttttaagt
    38041 tttacgtatt aatagctggt ggatacttaa attttgaata tcatatattg aattacagtg
    38101 attctcactt ttcggtataa ataaaaagga tagaaaaaat tctatccttt ttatagtttt
    38161 ataaaattat aaattcataa ttttataagt ttataatttt ataaattaat tatcggtatc
    38221 tttatagatg ttttgcactg cttttatact attttttaca atttttttat ttgctggatt
    38281 catgttgttt atataaaatt ctacaatttt atttaacgtt tcatatattt ttttatcttc
    38341 aattgtcgca atcgtgctga ttgcagtgtg agtatctagt ggcattctta tagatttagg
    38401 taaatcttta gttgtgagtc gttttttgga ttgttctaga attttgcctg aatcagttgt
    38461 attattcaag gcttcagaat tttctggaac aatattttca ggtcgttgtg ttttattttt
    38521 tctttttatg gcatttaaac caattgattc atttttttca ttagtcattt tgaaaagctc
    38581 cttatcctaa aagattaata cgttgtatta cttcgtcact aacactttga taaacttgtt
    38641 ggactctttt atcatgaatg tcttttgggt tatcggttat gccagttcta tcataccttt
    38701 tcaatctctg cattgttttg atttttgtat tgaaaatatt cgattttcca aataatgact
    38761 ctgcgtcttc aataatatcg aggtctaaat cattaccgtt ttgaactagt accggtagca
    38821 agcctaaaat atctatttct gttccataat cattgtttaa ttgaattaaa tgttctatat
    38881 atttttcagc tccttttaac gagcgttcct gtgtttgcag aattacgata acatagtcac
    38941 ttgcgactaa agcagaatta gtaaatttat ttaattgtgg tggtacgtca atgataataa
    39001 aatcatagtt ctctttaatt tcatttaata aatttgagaa atgcgtatct ttagaataat
    39061 catcgtcata taattccgct aaataatcaa tatacttttc taaatcagta aatgatggta
    39121 atagatctaa attttctttc actgagatta gtgaattaga aattttatta ttttgaatag
    39181 aaacaaataa tgtttcttta aattcaggct ttattttgta tacattggtc atagtcgcaa
    39241 ataatagctc tgtagcatta gcttgcggat caagatcaac aactaagact ttgtaaccct
    39301 tgttagataa tgtataagct gttaacacag cattagttgt tttaccaaca ccacctttaa
    39361 aatttcctgt ggttaatact tttgtcattt gttaatacct cctgtttata attttataat
    39421 tttataaaaa tattatactg ctatcg
//
LOCUS       DAYYHS010000017        35747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000017 DAYYHS010000000
VERSION     DAYYHS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.017"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..1591)
                     /gene="abc-f"
                     /locus_tag="ACHA6A_000905"
     CDS             complement(8..1591)
                     /gene="abc-f"
                     /locus_tag="ACHA6A_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8969128.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1696..1824)
                     /locus_tag="ACHA6A_000906"
     CDS             complement(1696..1824)
                     /locus_tag="ACHA6A_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969129.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2169..2312)
                     /locus_tag="ACHA6A_000907"
     CDS             complement(2169..2312)
                     /locus_tag="ACHA6A_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8969130.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2535..3014)
                     /locus_tag="ACHA6A_000908"
     CDS             complement(2535..3014)
                     /locus_tag="ACHA6A_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8969131.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3087..3371)
                     /locus_tag="ACHA6A_000909"
     CDS             complement(3087..3371)
                     /locus_tag="ACHA6A_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969132.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3449..3643)
                     /gene="ssrS"
                     /locus_tag="ACHA6A_000910"
     ncRNA           complement(3449..3643)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA6A_000910"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3715..5001)
                     /locus_tag="ACHA6A_000911"
     CDS             complement(3715..5001)
                     /locus_tag="ACHA6A_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8969133.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5139..5810
                     /locus_tag="ACHA6A_000912"
     CDS             5139..5810
                     /locus_tag="ACHA6A_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8969134.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5776..6264
                     /locus_tag="ACHA6A_000913"
     CDS             5776..6264
                     /locus_tag="ACHA6A_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8969135.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6277..7224
                     /gene="prmA"
                     /locus_tag="ACHA6A_000914"
     CDS             6277..7224
                     /gene="prmA"
                     /locus_tag="ACHA6A_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8969136.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7225..7989
                     /locus_tag="ACHA6A_000915"
     CDS             7225..7989
                     /locus_tag="ACHA6A_000915"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8969137.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8222..10435
                     /locus_tag="ACHA6A_000916"
     CDS             8222..10435
                     /locus_tag="ACHA6A_000916"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8969138.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10446..10892
                     /gene="dtd"
                     /locus_tag="ACHA6A_000917"
     CDS             10446..10892
                     /gene="dtd"
                     /locus_tag="ACHA6A_000917"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8969139.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11063..12322
                     /locus_tag="ACHA6A_000918"
     CDS             11063..12322
                     /locus_tag="ACHA6A_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8969140.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12411..13358)
                     /locus_tag="ACHA6A_000919"
     CDS             complement(12411..13358)
                     /locus_tag="ACHA6A_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8969141.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13752..15044
                     /gene="hisS"
                     /locus_tag="ACHA6A_000920"
     CDS             13752..15044
                     /gene="hisS"
                     /locus_tag="ACHA6A_000920"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8969142.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15059..16828
                     /gene="aspS"
                     /locus_tag="ACHA6A_000921"
     CDS             15059..16828
                     /gene="aspS"
                     /locus_tag="ACHA6A_000921"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8969143.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16937..17812
                     /locus_tag="ACHA6A_000922"
     CDS             16937..17812
                     /locus_tag="ACHA6A_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8969144.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18078..19961
                     /locus_tag="ACHA6A_000923"
     CDS             18078..19961
                     /locus_tag="ACHA6A_000923"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8969145.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20036..20320)
                     /locus_tag="ACHA6A_000924"
     CDS             complement(20036..20320)
                     /locus_tag="ACHA6A_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8969146.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20310..21044)
                     /locus_tag="ACHA6A_000925"
     CDS             complement(20310..21044)
                     /locus_tag="ACHA6A_000925"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8969147.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21173..21796
                     /locus_tag="ACHA6A_000926"
     CDS             21173..21796
                     /locus_tag="ACHA6A_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8969148.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            21988..23109
                     /locus_tag="ACHA6A_000927"
     CDS             21988..23109
                     /locus_tag="ACHA6A_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8969149.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23116..26244
                     /locus_tag="ACHA6A_000928"
     CDS             23116..26244
                     /locus_tag="ACHA6A_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8969150.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26263..29457
                     /locus_tag="ACHA6A_000929"
     CDS             26263..29457
                     /locus_tag="ACHA6A_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8969151.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29603..30310)
                     /locus_tag="ACHA6A_000930"
     CDS             complement(29603..30310)
                     /locus_tag="ACHA6A_000930"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8969152.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            31075..31473
                     /gene="spxA"
                     /locus_tag="ACHA6A_000931"
     CDS             31075..31473
                     /gene="spxA"
                     /locus_tag="ACHA6A_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8969153.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            31805..32458
                     /locus_tag="ACHA6A_000932"
     CDS             31805..32458
                     /locus_tag="ACHA6A_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8969154.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            32684..33682
                     /locus_tag="ACHA6A_000933"
     CDS             32684..33682
                     /locus_tag="ACHA6A_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8969155.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            33858..35666
                     /gene="pepF"
                     /locus_tag="ACHA6A_000934"
     CDS             33858..35666
                     /gene="pepF"
                     /locus_tag="ACHA6A_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8969156.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        11769 a         6340 c         7543 g        10095 t
ORIGIN      
        1 aaacgtccta gtcaatcagt tggccgagcg gctggcggct gatctgctcc tgatcaataa
       61 cgagtgcttc ttggaagtgt tgctgcaaaa agtagcggtc atggctgact gctaggagcg
      121 ttccttgata gtcttgcagc atttcttcaa tttcttctcg agcatagctg tcgaggtgat
      181 tggttggttc atccaaaatc aggacgttga ttttttctaa aaagagtttc agcaacgcca
      241 aacggatttt ttcaccaccg gataaatctt tgatccgttt acccacatcg ctttgataaa
      301 agccactttt ggctaattgc cgtcgagcga cttcttcgtt tccagtcaat gtgatggcgt
      361 aagccagcaa acggctatcg ggatcggcaa actgcagctg ctgcggcaaa taaccgattt
      421 gcaagctact acctaatttg atggtgccac cgtaaggctt ttcctcaccg agtaataatt
      481 tgagtaaggt tgattttcct gcaccattgt cgccgatgat ggctaaatga tctccacgat
      541 agactgcaaa actgctgtct ttgaatagta cttgatcagc gtactgctgg tgcaagtttt
      601 gaatgatgaa aacttctttg ccagattttc caccattaga caagctttgc agccgatttt
      661 tcgggggagg tggcggtttg acgagtgtca gtttcgccag gcggcgttcc agttctttgg
      721 cttttttgaa gaaggactca ttgtctcctt catgtgccca ctgacgaaaa cgacgaatca
      781 tgactttcaa ccgctggact tctttttgct gcagttcgta attttttgtc agctcagcca
      841 agcgagcttc tttcaagact cgaaaacggc tgtagtttcc gggatactca atcaatgcac
      901 cttcttccag ttcccatgtt tttgtggtga cttgatcgag aaattgccga tcatgagaaa
      961 cgaccagaac cgattttttt gtattctgta aatagttttc caaccagcga atactgttaa
     1021 gatctaaatg attggtcggt tcatcgagta aaagtaaatc ggcatcggct gctaaaattt
     1081 tggctaaagc gactttaact cgttcgcctc cgctaagttc tgttaaccgt gcttcctgac
     1141 ggtgtggaat ccctaagcct tgcaagatcg ctgcaatccg gtcttctaat acgtaaccgc
     1201 ctagttcttc aaagcgatgc tgcagctcac cgtaacgggc cagacacttc tctaagtttg
     1261 tatctggttc tgtcatcaat gcttcgtagc gactcatttg ctgtttgagg tcctgtatct
     1321 cagcggcaga atggagcaag tagtggttga cggtcgcttc ggaaactgta agttcttgtt
     1381 cgaccgtccc gatctttaag ccttttttgc gactgataac accgctgtcg acaccttctc
     1441 tgccggtcaa gatttgtaaa aaggttgatt tgccagttcc gttgataccg actaagacaa
     1501 ttttttcacc agcttcaatt tgacaggata aatcctcaaa taatggattc ccgccgattg
     1561 tttttttaat agaaattgct tgtatgatca tgatctctct ccttgagacc actcaaaaag
     1621 gccatggtta tccatggcct gtgttattta aactcagatt atcatgagaa aaataccttg
     1681 agatggccat tgattttacg tgaattcaac tgttttggac aaacttgtcc cgtcaacagc
     1741 aactgtaaaa ccaattgcat ggctaaatgc gatatggtat cgttggtaat tttcattact
     1801 tgttatttta gtcatgcgca tcatagtatt ttccctcctt cgtgtagttg tcttttccag
     1861 tgtacctgaa agtgtcagaa agttcaacta gcgctgacat ttacttaata tttatcgatg
     1921 tgtaattctg tcagagagct ttttttatct gactgagggt aatgtctcgt ggaccacatc
     1981 attgtattct gagctaaacc agcgttcagg cgaaatcata tggtgtttga taaaaaactt
     2041 tgtctacatg acttcctttt gccatttgct taataccgtt gcattaaaca gctcttctct
     2101 ttggaaatct caccaacatc gcgttcagga gtatcgttgt ggttgcaatg gcaagcggta
     2161 gcctttcatc aattcttgaa tgatttaccg aatgtagcac ttgccttctc gcggtacccg
     2221 aacccgaaat aaaattttag tttggcgata aaatcggtat ccgccatcga tacttcatat
     2281 tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc tttggcatgc
     2341 ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact tttttttatt
     2401 ttacttgaac tcattgactg ttttcccttt attttaaaca ttataaagac cgatgagtgc
     2461 ttgccgcgtc ttttgttttt atcacggttt tttgtgcaaa aaaacctgcc gatagttggc
     2521 aggtcgtcta cgtgttaagc tttggtgtgg tctttagctg gcaattggtt ttctaaatca
     2581 gttcggacaa caagcacatc gcaagctgcg tttctaataa catattcaga aacagaacca
     2641 ataaataaac gttctacagc attcaatcct gtggctccta acataatcaa atctacttga
     2701 ttatcttctg ggatttctct ggcaataatt tgttttggtg agccatattc tacgacagag
     2761 gttacattat tcaagccagc tttcttcgca ttgctttcat aatctgccaa tgtttgcttt
     2821 gccatttcag tagcttgttc tgcaagcatt ccatcaaagg atgaaacggt ttggaaagaa
     2881 cgtgtgtcaa tgacatgtgc taatagaagt tctccgttat ttcggatagc tacattgacc
     2941 gcctttttaa aagctaattc agcttcatca gaaccatcta cggcaaccat gatttttttg
     3001 tattgttgta acataagcat tcactcctca agtcaatcgt attgtactct tttattgtag
     3061 aacactttag ggaaaaagag aattatttac tgctttggtt cagcaatttt ttttgcaaat
     3121 cggatactga aggtggcaga aacaataatt acgatcagta aaaagaccaa ggcaataaag
     3181 cgttgattga aaaaagctgt taaaataccc attacgccta aaacagcata tgcagctccg
     3241 taaaattgta ggaatccttg atttttatca tttttgttga tcaatacaaa aaagatatcc
     3301 tgtttattaa ataaataata gctaacgaat aataatacta aagtggcgat aatcattaaa
     3361 atcctaatca tgaacaaatc ctccaaaata agcaacaaat agaaaaagca atttgccaag
     3421 agcaaattgc tttattcttt aaaagatggt aatctccgtt aatgccgttg tttgtcctgt
     3481 agtattgatc ccgcgaattc aagcaggtgg gtcccatggt cgtagcttcg aactaccaaa
     3541 tgataaggca tgttaccagc agtgacacaa gacgggtccc aagatatcaa taaaaatgtt
     3601 cggtcaacac acaaagagga caattcgaac atcacagatt tcccacctct atagtagcat
     3661 atgaatttaa aaagagcaat gagagagctc gcagaaacag aaccgttttc ttgtctagtt
     3721 ttttggagga tgctttttcc actcttttat acggtaatac tgttgaccca gagcctgttc
     3781 atatttccct gtatcttttg gctgataata ctgagcattc ttcagtttat ctggtaaata
     3841 ttgctgattg acccaagcct gatcaaaatg atgaggatac tgataaccca cgccacggtt
     3901 gagtgatttc gcacctttgt aatggctatc tctcaaatga tcaggaacat ctccagcttt
     3961 gccctctcga atatcagcaa gtgctgcatc taatgccatg taggcagaat tggattttgg
     4021 cgacaagcaa agatcgacga cgacgtctgc tagtgggata cgtgcctcag gcaatcccaa
     4081 tttttcagca gctaatacag cgttcactgt acgagccgca gcagcaggat tacctaaacc
     4141 tatatcttca taaccgataa ccatcagacg ccggcaaata cttgccagat cacctgcctc
     4201 aactaaacgg gcgagataat gaagtgcagc atccacatca cttccacgaa tcgatttttg
     4261 aaaagccgaa ataacgtcat aatgagcatc tccatttttg tcatgggtaa gagcttttct
     4321 ttggacacat tcttcaatga tcgacagtgt aagatgaatc cttccttctt tatctgaagg
     4381 agtcgatagt gtagccagtt ccagcccatt caatgcactc cgcaaatcgc cgttcgttgc
     4441 acgcgaaagg tgaagaagag catcttcgtc taactggatt gcttgttgtc caaggccgcg
     4501 ttctttatcc cttaaagcat gttcgactgc caactggatg tcttgttctg ttaatggttt
     4561 gacttcaaag atctgtgtac ggctgcggat cgctggattg atggtaatat aaggattctc
     4621 agtagtcgct ccgatcaaga tgattcggcc actttctaaa tgaggaagca gaaaatcctg
     4681 ttttgtttta tctaatcggt gtacttcatc caataagagg atcactgtac cactcatttt
     4741 tgcttcttcc gctacaactt gtagatcctt ttttgaatct gtagcggcgt ttagcatgcg
     4801 aaatgcataa ttagtagagc cggcgatcgc acttgcgata ctcgtttttc cagtaccagg
     4861 aggtccatat agaatcatgg aagagagcat gcgagcatcg accatgcggc gtatgatttt
     4921 tccttccccg actagatgct gctgaccaac gacttcatcg atcgttcgag gtctcatgcg
     4981 ataagccaat ggctgttgca taggattttc ctccttcttg tttttacgtc ccttaattat
     5041 atcatattta gcaagatgcc tagaaagaac agccttctgt tgttttgttt tcatagctta
     5101 tccaactaat attgtgtatg ataaagtagg tgagaaaaat gacggataaa caaaagatag
     5161 atgaattttt ttggaataaa acgacgccag aagtggcacg ggatttattg gggatgtatt
     5221 tggagcatga cacaccagaa ggaagattag ctggatatat tgtagacgcg gaagcatacc
     5281 taggtccaga agacgaggcc gcccatagct atggtttaag aagaacaccg cgtgtgcggg
     5341 caatgtatga aaaaccaggt acgatctacc tttacacgat gcatactcat cggattttga
     5401 acatcattac tcagccagaa gggattcctc aaggagtaat gatacgtgca atcgaacccg
     5461 ccgcaatgat tgatcaaatg agtaaaaatc gtggcggaaa aactggtcca gatatcagca
     5521 acggtccggg aaaattagtg gaagcattag caattcctca agaactttat ggtcagtcta
     5581 tagcagatag ttcgttacgc ttagtttttg agaaaaaaaa gacaccaaaa aagatcaatg
     5641 ctttgcctag aatcggtatc ccaaacaaag gtgtatggac agaaaagccg ttgcgttttg
     5701 ttgtgtcggg aaatccttat atctctttac aaagaaaaaa ccaaatcgag aaagattggg
     5761 gttggaggaa agaaaatgaa aaagaaggat ctattaacat atttagatga aataattgaa
     5821 aaacaagtga cagactatga tgtagcttta gattgggata caaaaaatca tacgatcgag
     5881 atcgttgttc gcttgtttgc agaaaataaa gcacaatttg agattgatga tgcagaaggg
     5941 atcgtttcgc aagaaccaat tatcgaattt gaagacggcg tacttctttt taatcctcag
     6001 aaatctgttt ttgatgaacg caattatcta gcagtaatcc cttatgaagg aaaaaaaggg
     6061 ttggcaaaat cagtagctga ttctttagtg gaatatctaa atgaagtatt agctcaaggg
     6121 caaagcgatt tgcttgattt tttggatgaa gacgatgacg aagctgtatt cgagcttcac
     6181 tgggataatc agcaactagc agaattagta gaagtaaaac agcaaaatga agcagatact
     6241 tatttaccgt acccaagtta ttaaggagtg acagatatga aatggaatga agtaaaagta
     6301 gaaactgaaa gtgaagcagt cgaagcagtt gctaatattt tgatggaggc aggggcgagt
     6361 ggtgtcgcga ttgaagattc tttagatgtt gaacattttc agtcggatca atatggtgag
     6421 attttagata aaaatgattt tacatcgctg aaagaaggcg cactagtcat ggcttatttt
     6481 ccggaaacga ctttcttacc ggaaatcttg ccttttatca aagatcgtat cacacaatta
     6541 cctgagtttg gtctagctat tggaaaaaat aaagtaacag ttagtgaagt agaggaaagc
     6601 aactgggcaa cagcctggaa gaaatactat catccggtgc gtattacgcg aatgctgacg
     6661 gttgcgccaa gctgggaaaa ctaccagaca gaagatcctc tagaaaaaat tatcacacta
     6721 gatcctggta tggcatttgg cacaggcact catcccacga cttccttgac attgacagca
     6781 ctagaaacag tcttgcgtgg tggtgaaaca ttgctagatg tgggaactgg gtcgggcgtg
     6841 ctaagtattg cagcaaaata tcttggtgca aaagatgtct atgcctttga tttagacgaa
     6901 gtagccgttc gttcggcgaa agaaaatatg gatatgaatg aaatagccaa agatgtccat
     6961 gtatcagcta acgatctttt gacaggaatc gagattgaaa gtgatgtaat cgttgcgaac
     7021 attttagcag atatcatctt attgatgatc ccagatgctt ggcgtttact gaagcaaaca
     7081 ggcacgctga tcgtttcagg aatcattgaa gagaagaagc aaatggttct ggatgccatg
     7141 ttagaacaag ggtttatcgt ggatcaaatt ttccagcaaa aagactggta tgcaattatt
     7201 ttgaaaaaac cagaggtgga atagatgcag cggtattttt taaaagaatc atatgaatca
     7261 aaagactatt ttgttttaga aggggaaaac tatcaccaca ttgtacgggt tatgaggatg
     7321 gaacccgagg atgaagtttt tttggcattc aatgatcaaa tagccattgt tgcagaaatc
     7381 acggagatta ctgaagaggc tgtttatctg aaagaaatta aaaaagaaga aaatcaaaag
     7441 gaactgccaa gttcgattac catagcgagt ggctatccta aaggggaaaa gctggattgg
     7501 atcgttcaga aatccactga attaggagcg caagcttttc ttggtttccc ggcatcagcc
     7561 tctgtcgtta aatgggatgc aaaaaaacgt atgaaacgac aacaacggct tgaaaagatt
     7621 gctcaagagg cagcggaaca atcccatcgg cagatcgtgc catcagtaga gttattggaa
     7681 acgaaacaag atttatttga gcggctagat gattatgacg tggtcttggt tgcttatgag
     7741 gaatcagcaa agcaaggcga aaaatcaaat gcagccaaaa ttcttagtga aacaaaacca
     7801 gatagtcgaa tcttagctgt ctttggacca gaaggtggat tatcccgcga tgaagtcgat
     7861 tattttgtag aacacaaagg gatactttgt ggattaggac caagaatcct tcgtacagaa
     7921 acagcacctc tttatttatt aagtgctgct agctatcaat gggaattaat gaatccttct
     7981 ttcagctaac gttcttcatt aataaaagag tatcgacaga aagaattgaa aaaatagtca
     8041 acaatttgta taatagaaac aattatttat acaatggata cgaaaaatcg tttgaaaaag
     8101 cttttaaaaa gcgaacattt ccgtaaaaaa acgtataact gcacggattt tgggacaatt
     8161 atcaaacaga tatttgtccc gattcccgtt ttaccgaaaa aagaaagggg gatcgattcc
     8221 tgtgacaaaa gaagaaattt taacgggacc cggtgtcata aagctcgtta gtcaatacat
     8281 gggtcccgaa catgtagcat ttgttgagaa agcatgcgaa tacgcgactg ctgcacatga
     8341 cggacagttt agaaaatcag gcgaacccta cattattcat cctatccaag tcgcaggtat
     8401 attagcagat ttaaaaatgg atccccatac agtggctaca ggcttcttac atgatgttgt
     8461 tgaagataca gaaatcactt tagaagatct gagagaagaa tttggcgatg acgttgctat
     8521 gttagtggac ggcgtaacca aattagggaa aataaaatat aaatcccacg aagagcagct
     8581 agcagaaaac caccgaaaga tgctgctagc aatggctcaa gatttacgag ttatcatggt
     8641 caaattagct gatagactac ataacatgcg tacgttgaag cacctgcgag aagataagca
     8701 aagaaggatc gctcaggaaa ctttagaaat ctatgcgcct cttgcacatc gtctagggat
     8761 cagccggatc aaatgggaat tagaagatac agcacttcgt tatctaaatc caaaacagta
     8821 ttaccgtatc gtccacttga tgcagacgaa gagagaagaa cgtgaaaaat acgtaagtgg
     8881 tactgttgaa gatattcgaa tagcgacaga agagctgggg atttttgcag aaatctatgg
     8941 acggccaaaa cacatttatt caatctatcg taaaatgaaa gatcagaaaa agcagttcaa
     9001 cgaaatttat gacttgctag ctattcgagt gatcgtagat tcaatcaagg attgttatgc
     9061 tgtattagga gcaatccaca caaaatggaa accaatgccc ggcagattta aggattatat
     9121 tgccatgcca aaagctaata tgtaccaatc tttgcatact accgtgatcg gtccggcagg
     9181 aaatccggta gaaattcaaa tccgaacaca ggaaatgcat gaaatcgctg aattcggggt
     9241 tgctgcacac tgggcctata aagaaggaaa aaatgaaaaa gtagaaccag atggtatgac
     9301 gaaacaatta agctggttcc atgagatact cgaacttcaa gacgaaagct atgatgcttc
     9361 tgaatttatg gaaggcgtaa aaggagatat ctttagtgat aaagtctacg tcttcacacc
     9421 aaaaggagac gttactgagt taccaaaagg atccggacca ttggactttg catacagtat
     9481 ccatacagat atcggtaaca aaaccactgg tgcaaaagta aatggcaaaa tggtgcagct
     9541 tgattacaaa ttgaaaaacg gagatatcat tgagatcatg acttctccaa attcatttgg
     9601 cccaagtcgc gactggttga aattagttgc tactagcaaa gcaagaaata agatcaaacg
     9661 tttcttcaaa gcccaagatc gagaagaaaa tgtgatcaaa ggccacgaat ccgtggtcaa
     9721 atgtattaca gatctaggat ttacgcctaa agatattttg acgaagaaca aactgcaaga
     9781 agcactcgat cgttttaatt atcaaacaga agatgatctc tatgcagctg tagggtatgg
     9841 agaagttagc cccttgacga tggccaatcg tctgactgaa aaagaacgta aagaacaaaa
     9901 aatcgagcag caaaagcaag aagcagaaga aatcatgaat cagccgaaaa aagaacctga
     9961 caaaatgaaa gtacgtcatg aaggtggcgt tgtcattcaa ggggtagaaa acttactaat
    10021 tcgtatcagt cgctgctgta atccgattcc tggtgatgat atcgttggtt atatcactaa
    10081 aggcagaggg atatccattc atcgtcgaga ttgtccgaat gttcagcctg acaaaccaaa
    10141 tgtagcagaa cgtttgattg aagtcgaatg ggaagataca tcgaatacac gaaaagagta
    10201 tgatgcagat ttggaaattt acggctataa tcgttcaggc ttattgaatg atgtacttca
    10261 aacagtcaat gcgctaacga aaaatctcaa cagcgttgaa gcacggacga ataaagataa
    10321 aatggcgacg atccatttga cggttggtat ccagaattta tcccacctaa agagtatcgt
    10381 ggataaaatc aaagcagtac ctgatgtcta cagtgtacgc cggacgaatg gataggagga
    10441 cagatatgag agcagttgtt caacgggtaa gtaaagctgc tgtgtcagtc gatcagcaag
    10501 aaattggaaa aattggctac ggcctacttg ttttattagg cattcatgaa accgatacgc
    10561 aaaaagatgt ggactatctg gtaaaaaaaa ttgcacaaat gcgtattttt gaagatgaac
    10621 aaggaaaaat gaatattagt gtagaagatg tgggaggaca aattttaagt atctctcaat
    10681 tcacactgtt tgcggatacg aaaaaaggaa atcgaccaag ctttgtggaa gccgcacgac
    10741 cagaaacagc tattccttta tacgaatcat tcaatgaagg aatcagaagt cgcggcatca
    10801 cagtcgctac cggacaattc ggagcagata tgtcggtttc tctcgtcaat gatggacctg
    10861 taacgatcat tattgattct aaaaacaaat aaagtaatct ggttttcact agtaaaaact
    10921 atgaaacacg tcgcttaagt tagactgata aactctaact taagtggctt ttttgtctgc
    10981 aaaaagcgct tcttttgtca gcagtcggaa aaaaagttaa gcttaaatta tacaagttat
    11041 tttttatagg aggttgctga agatgaaaga agtaccagta gtagatatca aaatcacaga
    11101 tttaaaaaaa ttgtatcaaa aaggatacaa catgttggag gagttacgcc atgaagcgga
    11161 cgctcccgtc gtcaaagcaa agattttcaa taaagaagct atcacgatct acggatcatc
    11221 agcagcaaaa gtattttatg atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt
    11281 agtattgaag acactttttg gtcaaggtgg agtacagact ttggatggag ctgctcatca
    11341 ccatcggaaa aatattttta tggatctaat gacaccggaa agaatggaag attatcatcg
    11401 tatcttagat aagaatctga cacaagcact tgaagcacag catggtcaat ttgaactatt
    11461 tgatttaagt aagatggttt tctttacatc tatttgcgag tgggcaggta tcaatttatc
    11521 agcgatttct aaagatgagg tcgaaaaatt agcagaatat caaatctcta tgatcagcgg
    11581 aacgtttact tctccgattg atcacataaa aggtgtcgaa aatcgcaaaa aatcagaaaa
    11641 atgggcgcaa ggcttgattg aagaagctcg acaaaatcct gtggctggaa aagaaaatgt
    11701 ggcattatat gcttttgcca atgccacaga tttagatggt cagcttttac ctctggaagt
    11761 ggcagctgtt gaattattga acatcatccg tccaacggtt gctctaactg tgtgggcggc
    11821 attgatggga catgcacttt tcagtagacc agatttgtat cagcaattaa aaaatgactt
    11881 ttcgacgtta caagatccgt ttattcaaga aatgagacgc tattatcctt tcttcccaat
    11941 gctacctgcg atttcgctga aagaagtaga agtagatggt tatcgtatac cagaaggcag
    12001 ttgggttatt ttagatttat atggaacaga ccatgatgaa cgaacagttg aagcgccaga
    12061 ttcctttatg atcaaacggt atgtagggaa agcaaaagat atttcctata aagaagaata
    12121 tgagatgatt gcacaaggcg ggggaaattt tcgacaaatg catcgctgtg caggagaatg
    12181 gatcactctt catagtctac gtgtgttttc cgatcagcta gtaaataagt ttgagttctc
    12241 tgtacctgaa caagattgga cgattccatt caatcagttt ccgacttatc cgaatagccg
    12301 agcgctactt tacaagaatt agattagcat aaacttatcg aaaaaaagga agatcaaaca
    12361 gtgctgatct tccttttttc atattactat aaattgataa ggtcaattga tcaaaatcca
    12421 cgaaatgctt catcaatggt ttgataatct tcattggata aatgcaaatc taaagcttgg
    12481 acattgtcag atacttgctc tggacgctta gctccaggta taactacaga cactcgtgga
    12541 ttcttcatgt accaagctaa aacaagttgc gcaggagtgg cttggtatct tttagcaatg
    12601 gggcgaagta catcgacagc ggttaaaatt tctctgaaac gttctccttg gaaatctgga
    12661 tttctttttc gaggatctcc ttcaccaaat tgtttttcct caccaagttc atattttcct
    12721 gtcagcaaac cggaagcaag aggaaaatag ggaacaaaac tgattttgtt tttttcaaga
    12781 taagggaata attctttttc agcctgccga tggatcaaac tgtatttatc ttctactaca
    12841 tcgacatatc catctgcatt cgcttctttc aattgttcta acgtgaagtt tgacacaccg
    12901 actgctcgga tttttcctgc ttcttttaat tcgtgcaatg tagcaactga ttcatttttt
    12961 ggtgtgcttt catccggaaa gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt
    13021 tttagtgcat cttccaccga ttgtttgaga aactcaggag aattatcaaa tgttcgccct
    13081 ttatttggta catgagcagc ttttgtagcg atgacgacac gtgagcgatc atattctttt
    13141 tccttcagta cctcgccaat cagttcttcg gaacggccgt ttccgtaagc ataagcggta
    13201 tcgatcaaat tgatcccgct attcaaagct gtacgaacta cttgttttcc tgtctcatct
    13261 tccaaaccgt caaaaagatt atgtcctcca acggcatttg ctcctaatcc aagctgttct
    13321 gcgtagactt gtgaatgtcc gattcttatt tctgccatag ctgattcctc ctttttgtct
    13381 agtctaaaac aaaataccaa aaagctgaac taaaacgttt ttgtgagggg aaatagtcag
    13441 atatctcttg actttgagac tggtttttag taggttataa gtaatatcga atgtcaatga
    13501 aagagcgagt aagtttttga aactttacaa gagaggatca tcgaggctga aaatgatcta
    13561 aagtttcatt agcctaaaga cactctggag atggacgagt gaaaagccgt cccgcgtacg
    13621 agcgttattc gtaagaggaa agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt
    13681 tcattcaccc ttgttcatga tgaacaaggg tgattttttt atttacatag ctaagaaagg
    13741 gagagaaaac tatgagtttc caaagaccaa aaggaacaaa tgatattttg ccaggcgaat
    13801 cagaaaaatg gcagtttgtc gaagaaacag cacgtttatt attcagagat tatcagtata
    13861 atgaaatgcg tacaccgatt tttgaacact atgaagtgat atcccgaagt gtaggggata
    13921 caacggatat cgtttcaaaa gagatgtatg atttttatga caaaggggaa cgccatgtga
    13981 cgcttcgacc agaaggtaca gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg
    14041 gcccagagta tgcgaaaccg ttcaaaactt tttatatggg accaatgttt cgctacgaac
    14101 gccctcaaaa aggacggtta cgtcaattcc atcaaatcgg agtagaagca tttggatcag
    14161 aaaaccctgc aactgatgtg gagagcatgg tgatggcact tgatttcttc aaacagttgg
    14221 ggatcacgca aatccggcta gtcatcaatt cattaggtga taaagaaacg cgtcaagcct
    14281 atcgtcaagc attgatcgac tatcttttac cttttgaatc agagctgagt gaagactcta
    14341 aacgccgcct tcatgaaaat ccattacgcg tattggacag taaagacaaa cgagatcaaa
    14401 aatttgtggc agaagctcca tctatcttag attatctaag cgagcctgct caaaaacatt
    14461 ttgataccgt tgtttccatg ctggatgcat tggctgttcc ttatgaaatc gacagtaata
    14521 tggttcgcgg actagattat tacacacata cgatttttga aatcatgagt gacgcaccaa
    14581 aaatgggggc acaagcaacg atttgcgcag gcggacgtta tgacaatcta gtggaagaac
    14641 taggtggtcc ttcaacacct ggcttcggat ttgcaatggg tatcgagcga ttactcttga
    14701 ctatggaggc agaggaagta gttatccctg catttaatga gttggatgct tacgtggtcg
    14761 cactcggaga cgaaacaaat atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg
    14821 gtttttcagc tgaccgcgac ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag
    14881 ataaagcgaa tgccaaatta gtattggtga tcggtgaaga tgagcttgct agtcaaacga
    14941 ttaatgtcaa agcaatggca agccgaaaag aaaaaagttt tccattagaa gagatatatg
    15001 aacattttga tgaagtctat gatgaaatga ctttattcga gtaggaggaa aaaaataaat
    15061 ggcacaaaga acgatctatt gcggattagt ttccgaacaa caattagaac aaacagttac
    15121 attaaaagga tgggtacaaa aaagacgaga ccttggggga gtaatcttta tcgacctacg
    15181 tgatcgtgaa ggaatcgttc aaatcgtctt taatcctgaa atcaacaaag aagcatggga
    15241 aatcgcggat aaatgtcgca gtgaatacgt gatcgaagtg actggggaag ttagaagacg
    15301 cggagaaaat gcagtaaatc ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga
    15361 aatcgtgatt ttgaatcaag caaaaacccc gccatttcaa attgaagatg atcaagcaat
    15421 cagtgacgaa atacgtttga aatatcgtta tttggatctg cgtcgtccaa aaatgactaa
    15481 aaacatcaaa ttaagaaatg cgacaacaaa agcgattcgt cattatttag atgataatga
    15541 ctttctcgat atcgaaacac cttacctagg gaaatctaca ccagaaggcg caagagacta
    15601 cctagttcct tcacgtgtcc atgctggaca tttctatgcg ttgccgcaat ctcctcaatt
    15661 gtttaaacaa ttgctgatga atgctggatt cgaccgttat tatcaaatcg ttcgttgttt
    15721 ccgcgatgaa gatttacgtg gagaccgtca gcctgaattt acgcaagtag atattgaaac
    15781 aacgttctta acagcagaag aaatccaaac ttacacagag ggattgattg cgaaggtaat
    15841 gaaagaagtt cgcggtatcg aagtcacatt gccatttcct cgtatgacct atgatgaagc
    15901 aatggctcgc tacggcagcg acaaaccgga tacacgtttt gatatggaat tgatcgatct
    15961 aagcgataca gtcaaagaag tagaattcaa agtcttccaa atggctcttg aaaacggcgg
    16021 tgtcgtgaaa gcattgaatg ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga
    16081 tcagttaggt caatatgtag gacaattcgg agctaagggg cttgcatggc tgaaagtaga
    16141 agaagacgga ttgaaaggtc caattgctaa attcatggga gaagcaacag aagcaatcat
    16201 taaagcaaca gatgcaaaac caggtgactt gctgatgttt ggcgcagata aaagtgaaat
    16261 cgtcgctgct gcattaggtg ctatccggac acgtttagga aaagaattag gactaattga
    16321 tgaatcgaaa ttcaatttct tatgggtaac tgattggcca caatttgaat tttcagaaga
    16381 agaaggtcga tatgtctctg ctcaccatcc attcacgatg ccaaaagaag aagatatccc
    16441 gttattggct gaagaccctg caaaagtcta tgcagaagct tatgatatcg tcttgaacgg
    16501 ttatgaacta ggcggaggtt ctatccgtat tcatactcgt gagcttcaag aaaaagtact
    16561 agaaactcta ggctttacaa aagaacaaat ggaagaacaa tttggtttct tgttaacagc
    16621 tttagattac ggattccctc cacatggcgg tattgcatta ggattggacc gttttgcaat
    16681 gttattagcc ggagaagata atattcgtga agtcattgcc tttcctaaaa acggaaaagc
    16741 agcagatgtg atgagtgatg caccaagtat ggtttcgcct ttacaattgt tcgaattgaa
    16801 tattgatgta acgaatatag aagaatagcc gataaaaagg taaaaaggat ttgtcgaata
    16861 cgcttctttt gacaaccttg atttaattga aaaacacttt ataatgaagc cataacatat
    16921 aaagcggggc ttttccatga aaaaaacatt cgaaaattgg gcttatcatg attacacgac
    16981 caagctttcg gtatctatcg tttatgcgtt gatcgcatca gtcgcattga atttctttta
    17041 tcagccagga catatttatt caagtggtat tactggtcta gctcagattt tgacgacttt
    17101 gtcaaaatca ctgataggat tcgaagtacc ggtatcaaca actttgtatt tactgaatat
    17161 acctttattt gtattggcgt ggttaaaaat cgggaagaag tttacaattt tcacttttat
    17221 cacagtcact ttgacctccc tttgtatcca tattttaccg gaaacagtcc tatctaccga
    17281 tccaatcatc tgtgcgttgt ttgggggggc tgtgatggga ctaggtattg ggctagcgtt
    17341 gagaaacggc ctatcatcgg gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac
    17401 aggaaagtcg attggttcta tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg
    17461 aattctattt ggctggcaat atatgtttta cagtacgttg tctattttcg tcagtggaaa
    17521 agtaactgat gtcgtttata ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca
    17581 tccagaaaaa gtagtaaatg aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa
    17641 tgaagcagaa ggtgcttata agcatgataa gcagacagta ttgatgacga tcgttacacg
    17701 atacgaactg cctctactta gagaagcaat gaaagcttct gatccacaag cttttgtaag
    17761 tattgcagaa aacgtccaga tacttggtcg tttctatgag gatggcattt agatcgagtt
    17821 tgacaaaaat tcatgaaata ctgctgtaga cagacgttcg tttgtctgca gtttttttct
    17881 accatagtag aatcagcaaa gaaaaactgc ttttttattt gaaattttct taaagttgaa
    17941 aaaaagcatg tttatttttt taaatttatg ggatgataga aaaaaatagt agagaaagga
    18001 cacgattcta cttgctattt tcacttaata cccgtatatt acgaatgaaa aaagtttatg
    18061 aaaagttgga atattgaatg gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg
    18121 ggtttgatgg gctccgttca ttagctgtgg tgggtgttat cttctatcac cttctgccaa
    18181 ctagtatgaa aggcggctat ttgggcgttc cgattttttt cgtcgtatct ggttatttga
    18241 tcacggatct gctgcggcag gaatgggaac aaaatggaaa aatcaatatt tggcaatttt
    18301 atgtcagaag aatgaagaga ctctatccgg cacttgtttt tatgcttgtg acagcttcgg
    18361 cttatatcac tttgtttcag cgagggctgt taaacaatct tcgaggagtt gtggtcagca
    18421 gcttactgta tgtcaacaac tggtggcaaa tcaataacgg tttatcttat ttcgatcgat
    18481 ttgctaatga gtcaccgttt acgcatatct ggtctttagc agtagaaggt caaaactatt
    18541 tggtttggcc gatcttgttt gtattgttga tggtatttgt tcgtaaaaga aaatggatct
    18601 tttatactgt gttaggtaca tccttcctct ccgccatctt gatgatgatc ttgtatacac
    18661 caggaggcga tccaacccgt gtttattacg gtaccgacac tcgtctgttt tctatatggt
    18721 tgggaagtgc gcttgcgttt gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac
    18781 aagcgaaaaa tattctgaat atcgctggag gaatctcgct tttggcactg attctttgct
    18841 tcttcttttt agatgatcat tacaatttca tctattacgg aggaatgctc ctagtgagtc
    18901 tgttatgcgt agtgctagtt gcagtgacag cacaccccgg agcaagtttg aatcgatggc
    18961 tgacaaatcc agtatttact tggattggca aacgaagcta tggcatttat ctgtatcagt
    19021 ttccagttat gattttttat gaagcaaagg tgactgatat tgccgatcat gttttgttgc
    19081 atactttagt ggaaatcaca ttgattctaa tcatcagtga actttcttat cgatatgtgg
    19141 agcgtccgct agcacgtttt gattatggtc gtacattcca agtgatgaaa ggatggttca
    19201 gcaaaccgat catcagtaag agcaaaccat gggttgttcc aggcacatta cttgtagcaa
    19261 ttgcactcgt tggtttcgtt acggcaccga aaaacgctgt aacagccgat caaaaacaac
    19321 tgcaggcaaa aatcgctgaa agtaaaaaat tagcagaaga aaccagaaac aattcagaaa
    19381 acaccactag ttcggttgac caagcgatcc ttgataagta cgagttgtcc aaggcacaag
    19441 ggaataaggc acaaaaatta gaactaacgg catttggtga ttcagttatg ttggatgctg
    19501 caacggatct acaagaactt tatccaaaag tagtggtaga tggagatgtc ggacgacaat
    19561 tatatgccag tgtaccgtac attgaaaaat taaaggaaca agatttgttg aaagacactg
    19621 ttctgatcgg actaggaacg aatggctcat tctcggaagc tcagtttgat agtgtgatgg
    19681 atgcaatcgg tgatcgaaaa gtatactggg tcaatgtccg agtaccgacg aagcgctggc
    19741 aaaatgacgt gaatgcgatg cttgaacaga tgacgaaaaa atacgacaat atgacgctga
    19801 ttgattggta cagctacagt aatgaccata gtgactggtt ttatgatgat caagtccatc
    19861 caaatcctga agggatgaca cattacattc acttagtcag cgaagccttg ctaggaggaa
    19921 gttcaagtac gaacagcagt gagacaaacg tgacagaatg atagctacaa aaaacttcag
    19981 gcaaaaacat ttgtttttgc ctgaagtttt tttatattta ccgtgttttt aacattcata
    20041 tagattcagg aagcaaaacg gttgagtgtg tctgtaaata acgttctttt tgttttcggg
    20101 tgagtttttt gaatgcagct tctgcttttg ttgcttcgct tcttgaagaa aatacttctg
    20161 catggatcat cttaacaggt cttctttttt gaagacgagt atatttagca ccagtaccat
    20221 tgttatgttc ctctaatcgt ctagaaagat cggttgtata tccaccataa aatgtctgat
    20281 ctttacagaa taatacgtaa aaataatgtt catttgccat agagcatctc acgaatctct
    20341 gcagtgtatt ctccatccat attgtaagtg atcaatggtg gagcgacttt gaatccgtct
    20401 aatttgcctt ctttgattcc ttcaactaac aaaatatttg cctcttttcc aggttttggg
    20461 taaacaaatt ggatcctttt aggtgcaatt cgatagtctt ccatgacctg aatgatttct
    20521 aagaaccgat caggacgatg gaccatagta aagcgtccat ttgtttttag tagcttactt
    20581 gaaatatcga tcacttcttc taacgtcgta tgaatctcat gacgggcgat tgccaggtgt
    20641 ttattagggt tcgttttatt tgttggcagc cctttgaaat aaggtggatt acagacgact
    20701 aagtcacaag aattatgtcg aacgacagat aagctgtctt tcagatcgat ggtgtggaca
    20761 gtcatttgtt catttaaatg gttaagcgcg atactacgtc ttgccatgtc tgctagacga
    20821 ggttgaagtt cgatagcgtc gatagccgcc tgggttcgtt tacttaaaaa aagtccaact
    20881 gcgccatttc ctgcacaaag gtcaacaatc ttcccttttt taggtacacg ggaaaagtga
    20941 gcaagcagca cagcatctaa agaaaaagaa aacactttgg aactttggat aatcttgata
    21001 tcttctgcgt ataattggtc gattcgttcg tcttcgtgaa gcatggattc cctccaaaat
    21061 gaaaaaaata gtcgtctgta ttatactatt tttattaaaa agaaccaagg tggcttgtca
    21121 aacctctatt ttttatgcat actatgacca taagaaggga aaggaagaga ctatgtttta
    21181 tcgatttatg cgcggggtag ttcgagtact attagcctta gttaatggaa atgcccgcta
    21241 tgagaataaa gaagttttac cgcaagatga gaattacata ctagttgctc cccatcgtac
    21301 ttggtgggac ccattatatc tggcggtagc tgcagcaccc aaggaattca gctttatggc
    21361 aaaagaagaa ctgttcaaaa atccagttct gcgatttata ttaactcgat ccaatgcttt
    21421 tccagttaaa agagaaaatc caggaccaag cgtcattaaa acacctgtaa agattttgaa
    21481 atcgacaaat ttgagtttga tcatgtttcc cagtgggaca agacatgcta cagaacttaa
    21541 aggcggaatg gcattgattt ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca
    21601 aggtccgttg acacttgggg atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga
    21661 accaatcgac ttatcagata tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg
    21721 acgaacacaa gcagcttttg acaaattgga caaagaagtg aaccctgatt tcaaatatga
    21781 agtcaaaaaa aaataaaatg ataagtttat gcgtgggcgg tcttttgaat ctactgtcct
    21841 gttcagttca gactttttca atgaaaacga gggagagacg tcaagtctgc cctctttttg
    21901 cgttccaata tgtaaaatag aagacaggtg tcatgtaatg gatcaaagtg ttataataat
    21961 tagaaggaca ttttttgagg tgattgaatg cgttttttac atacagccga ttggcatatc
    22021 ggaaaaaaac tacaaggtta tgacttgatt gaagaccagc gtcatgtact atcttctata
    22081 ctagatatcg cgaaagaaga aaaagtagag gcaatcgtga ttgccggaga cttatatgat
    22141 aggagtgtcc cagctgttga tgccgtagaa ttatacaatg aattgatggt cgattggaat
    22201 ttaaaggaaa agatcccgat ttttgctata tcaggaaatc acgatagcag tacgagactt
    22261 tcagcgggta cgccatggtt cagttattcg aactattttt tgcgaacaca gctagcagaa
    22321 gcatttgaac cagtcgagtt taaaaacgta caatttttct ttcttcctta ctttgaacca
    22381 attgcagcga gattatattt tgaagatgac aatatccgaa cgatcaagca agcggtagcc
    22441 aaggtgatag ataaaatgca ggagctattt gattcagaaa aaaaacaagt attgattagc
    22501 catttttttg taatgggaag tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg
    22561 ttggattcga tccctggtga actacttaca ccgtttgatt atgtagcact aggacatttg
    22621 cataatcaag ctgctttgag acaggataat gctcgctata gcggttcccc attgaaattc
    22681 tctttatctg agatcaacca aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg
    22741 gagttgaatt ttagggagat cgaacccctg catgatattg aagaaataac agcgagtttc
    22801 aaagaattgc tggatcctgc cttttatcag tccgtcaatc gggagaatta catctatatc
    22861 tatctcactg atagaacgat catcccgaat atgatgaacc agcttcggca aatatatcct
    22921 cgtattttgg ggattgaacg ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa
    22981 gtactagaaa tgaaagatcc tcaaaaatta gcagaagcat tttttagtga aatgacggaa
    23041 gagcagctaa ctgcaaacca agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa
    23101 atggaatgat caagaatgaa accgaaaaaa ttacaactta aaaattttgg tccttttatc
    23161 aatgagaccg tagatttttc acgtttgaca gaagcaccgc tttttttgat cagcgggaag
    23221 acaggagctg gcaaaacgac gatttttgat gggatcacct tcgcactttt tggtgaaact
    23281 tccggaagac ttcgctctgg aaaagagatg cgttctttgt ttgctacacc agacgaagaa
    23341 acaagcgtga ctttttcttt tgaacatcag cagatgagct atgaaatcga acgaaaacca
    23401 gaacaagttt tagcaaaacg aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg
    23461 ttgaccattt ttgatgggaa aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca
    23521 gatcagttga tcagagaact gatgaatctc gatgcgaaac agttttctca aatcgtttta
    23581 ctaccccaag gagaattccg taattttttg gtttcatcaa gcagtgataa agagactgtt
    23641 ttacgtcatt tattcggtac tcaatttttc caacagttca atgagcgatt aaaagaaaaa
    23701 gcaaaacaac agcaacaaaa attggatcat ttagagcaag agctgcatct tctgcaaaaa
    23761 aggtttgttt tagtcaaaga agaggaacca gcagcagggt ttgaagccgt tttagcccaa
    23821 tggacaaacc atcaggcaat actcgaaaaa caaatcacgg aagaaaaaga agctctaacg
    23881 gaacttcagg aaaaacaaaa gcaactagaa aattcctact atcgttttga aagcttgaaa
    23941 aaaagttatg aagaaaaaac gatattaata aacaagcaaa aggaattagc tgctcaaaaa
    24001 gacgaaatag aggaaaagaa acgatggatt gggtattatg aatttacgca acgattagtc
    24061 gaaccaatcc agcgcctaca agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt
    24121 agtgagcaaa caaagaaact agcgatgact acgcagaatt atgaagagtg gcaaaaaaat
    24181 gaagcaaacc gcgtacgccg acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac
    24241 aaacagatga tggtgccaat tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa
    24301 ttaacaattt cattggataa agaagaattg aatgccgaga agctgtatca aaatctcgct
    24361 atctatcagg aaagaactcg aattcttcaa gaaaatttac tggatcagga aaaactgcaa
    24421 gaagatcgtc ttgcttttgg acagttaaaa tatctagatg aaaagcagca agaaatagaa
    24481 aaacaaaaag caaaagacac tgggtcgctc gcacagataa gagaagaact caaaaaagca
    24541 actgaagaaa taacaaaaaa tagtcagcta ttgaaagaac agacagaaca agcagaacag
    24601 ctgaaaagcc aatgggcaaa gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg
    24661 gagccatgtc ttgtttgtgg atcaacggaa caccccatac aacaattaaa acacgaacag
    24721 ccaaataagg aagatatctt aaaagtagaa caacaactgg cagaagctga gtctgctgta
    24781 gaaagaactc agcaaatcat tgccaaaagt gaagcacatg aaaataatct tcaacaacaa
    24841 ttatcagatt tagaacaaaa aatcatggca acgaatatac aattggctga acaagaagaa
    24901 cacttgcaac aggctttgaa aaaggcattt cctgagataa cagaacaaaa aacaaaaaca
    24961 actcttgtag taatcgaaca atctttagat agacgaaaaa gcaagatggc caatgcaaga
    25021 atagagctgg aagaaataag acaacaagca gaaaagctcg aacaacaatt agctgtctac
    25081 aaacagcagc agacagaaaa aaataatcag ctccagcaac tgcaaggaga aatcaaaagt
    25141 ttagaacaac aattgcaaga tacggatagt aactgtttag aagaggagat acgacgactt
    25201 gaaaaacagt tagaagaaga ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa
    25261 ttgttgcaag atcttcatac gcttcggaca cagcttgatc ttcaacatga acaaatagaa
    25321 aaaatagatg aaaaaataga aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag
    25381 tcgtattttt caacaattga agaaatctgt gcatttgcac aacagcaagc acaatataaa
    25441 gaaaaccaac gtacagttac ttcttatcat gaagagcaat tagtgacagc tgaccggctg
    25501 cgtcaattag aaaatatcca gctaactgag acattcccag atacagaaga gatcagccaa
    25561 cagttagaag aaataagacg tatgatcgaa ataaagcaag aaaagatata cggtttacaa
    25621 gaacaaaaaa ttagtaatca aaaaatctac gatgaatgtc tttcgattta tcacacaagc
    25681 cagacgaaat tagatgagct tacgcaatta cagcagcttt cgcaaacatt caacggagaa
    25741 aatcctaaga agactagttt ggaacggtat gtgcttcagg tttatcttca agaagtgtta
    25801 caagtagcaa atgatcatct ccaacgttta acgaaaaatc gttatcaatt tgagctagcg
    25861 gataccattg gcagttatcg agggaaaacc ggattagaaa tcaatatcta tgatgatcag
    25921 gcaggtatgt ctcgaagtgc gcataccctg tctggcggtg agagttttat tgcagcactt
    25981 tctttagcat tagctcttgc cgatgtgatc cagacacaag ctggtggtgt agcaatcgat
    26041 gcattgttta ttgatgaagg atttgggtca ttagatgaag aagctttgga aatggcaatg
    26101 gaagctttag aaacgatcga aagtgaagga agaatgatcg gcatcattag tcatgtaaga
    26161 gaattgaaag aacgagtatt acaacaaata agaattgata cagaaggtag cggacaaagc
    26221 aaaatacgct atcaacttgg ataaaaaggg agattggtaa ctatgaaatg gagtattcca
    26281 gaaaaagtca ttgcacgtgg acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc
    26341 cctgaccaag aagaaaacgt gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt
    26401 gatttggatg gtacagcaaa agagatagat tactgccaat gtccctattg ggaggaacat
    26461 aaatattgta agcatacagt tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt
    26521 atcatgaaaa aagaccagcc aatgctagaa ccggtaaaag caacaagtga gggagaaatc
    26581 attgcaaaag gtttttctcg tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg
    26641 attattgaat accaagtaga aacgattgag actaatcagt atcacccaga gctttctatt
    26701 ttggcacttt acttgcgtat cggttcattg gcatcaccga aaaaaacata tatcgtaaaa
    26761 aacatctatg attttttaca agcttacaca gaaaaagaaa cttacactgt taataaacag
    26821 taccagtttc gtctagataa acgggcgttt caaaaagaag atcaaaaaat actcacgcat
    26881 cttgcaggaa gtgctcagac gcatcaatta ttaggtacga acggcttaca agtcaaagga
    26941 aaactagata aaagatatct gcttctccca gtagaacaag cccgcttcct gttagagcag
    27001 atgaatcaga ctacacgttt gcatctgcag ataaatgaac aaaacgatca aggaatacaa
    27061 ttttccgatc aaaacaaacc tttatcattc aaagtggaaa aatttggaga agattatttg
    27121 ctgcaagcat tgaatgattt tgattttttc tttatgcatt atcagtgggg agttatccaa
    27181 gggacatttt attctctgac gaaatatcaa caaacggttt atcaaacttg gaaacaattg
    27241 attcgccgat tggagaaacc agaagtagtg ttcaagaaaa aagaattacc gtctttgttt
    27301 aaagagatta tccctctgct gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa
    27361 gaggaagtag cagatatccc cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa
    27421 atccaagctc gagtcgattt cgtctatggc gaagtcatct attcgactga cccgaagcat
    27481 gaggtctcga cagaacaggc ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta
    27541 cttgatctat tcagaatgta tcgctaccag aaaaatgaaa ctggttatga acgtccattg
    27601 ccaagcgggg aagaactata tgcctttttc cgcacagaac tatctgtctt ccgtcagttt
    27661 ggtgaagtca gacttggaag aaaattgcga gagttgtatt tagatgcgca taaacatcaa
    27721 ccgaaaatcg aagtaaccga gaccggttca tggctcgata ttcgattcga tgtgacaggg
    27781 atagaggagc aagaaatcga tcatgtcctg caaagcttgc tgagaaatga tgctttttat
    27841 acattagaaa atggtcaggt tctttctttt gattcagaag aatttcaaca aacaagccaa
    27901 gtgttgcaac aattacggga atcgatacgt acagaagaag gaacgatcca tgtacctaaa
    27961 aatcaaggat tgatcattca aaatcaattg gaaaaaagca atgctacttt tagtgaatcg
    28021 ttccaaacga tggtacaaga tctcattcat ccagaaagat accaggcaca gcttcccaaa
    28081 ggattgaatg caacgatgag agattatcaa aagcaaggtt ttcgatggtt gaagatgtta
    28141 ggtcattatc agtttggcgg tattttagcc gatgaaatgg gacttgggaa aacactacag
    28201 acaattgcct ttttactttc ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta
    28261 gcgcctgcga gcttaattta caactggcag gcagaggtga gaaaattcgc cccttcttta
    28321 agtatccaag taatcaatgg gaataaaaaa gaacgtgaag aattactggc taaagacaca
    28381 gatatccgag tgacttctta tgccagctta agacaggatc tagctaatta tcagtcgcaa
    28441 aaaattgatt atttgattct tgatgaggca caaatggtca aaaatagcag tacgaaaact
    28501 gcacaagcat tgagagaatt agctgtacct cagcggtttg cactgagtgg gacaccaatc
    28561 gagaataatt tagaagaatt gtggtcattg tttgcaacga ttatgccagg atttttcccg
    28621 actaaaacaa aattcaggga acttgccgca gaagaaattg cgcaaatgat tcgaccgttt
    28681 attttacggc gagataaaca aactgtgttg aaagacttgc cggaaaaaac agagatgaat
    28741 ctatattctg cacttacaga agaacaaaaa actgtttatt tagcttactt gcgtcaaatg
    28801 cgtgaagaaa tcacatcaat ggattctgaa gcattcaaga aaaaccggat cgggatactt
    28861 gctggtctga caagactgcg acaaatttgc tgcgatcctc gtctgtttat tgaagattac
    28921 caaggcggtt ccggcaagct cgaacaagta aaagatctac tggtagctgc caaagagaac
    28981 aaacgaagag ttttgctgtt ttctcaattt acagggatgc tgactatctt gcaagaagag
    29041 ttagccgagc tagggatctc gacattttat ttaagaggca gtacgaaacc acaagatcgg
    29101 ttaagtatgg tagacgcatt taatgctggc gaaaaagatg tttttctgat ttccttaaag
    29161 gctgggggaa ctggattgaa tctcacggga gcagatacgg tcatcttgta tgacctttgg
    29221 tggaatcctg ccattgaaga acaagctgct ggacgtgcac atcggatcgg acaaaaaaat
    29281 gtggtagaag tgtggcgtat gatctctgaa ggaacgattg aggaacgcat ggattcactc
    29341 cagcaagaaa aacgcgaact gttccaaaaa gtgatccaag gtaatgaaga acagttgacg
    29401 aaactgactg aagaagacat ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag
    29461 actcgaaaaa gcgttcagta atgggtaaga aagcctttct aaagaagcga ttgtttattt
    29521 gatttaaaaa atagcaagga ggctgggatt ccattttatc ccagcttcct tgctatcatt
    29581 tttttatttc tatttttaaa ggttatacaa agattgtctt cattgtctct aacagctgat
    29641 caaaagaaga aatctcgata tcgcaaattg gagtctgttc aattttgcgt tctcgatggt
    29701 tgaaccaaag cgcttgccag ttagcacttt ttgcacctag aacatcattg tcgtaattat
    29761 cgccaacgta taaggtctct tctggaagca tatgaaattc tttctcagct agctgaaaaa
    29821 tctctttttc tggtttttgg aaacctgttg cttgagaaat gagcatattg cgggaaggaa
    29881 tccaattgtt caattgaagt tgcttcagtt tttttgtctg atggtcagtc ggcccatttg
    29941 tgataattcc taatgggaca ttcttttcct tcaagaaatc gagggttttt ttgacctcct
    30001 catgcaagca gatattatca agttcttcct cgtaaatttt ttgaaataag aggccatctt
    30061 cttccgtaat atgagggtag tctaaatctt ttagtgattg gctgatccga tgtgctcgca
    30121 tatattctag tgtccaatct cctgccatta cttttgggaa gttttcatca ctatgatggc
    30181 ggaaccggat atatagtgga tgcatatctg catctgaaac aagcggaacg actcttttga
    30241 ccgcgtttcg aaatggctgc tgctgatcat acatcgtatc atcaacatca aacacaactg
    30301 atttaatcaa aattttacac gcctttcggt tttcattgat tttttcacaa atccttgact
    30361 attgtaacag aaaaaattgt ctaaaaaagc tgataaataa aggtttttct tactatattt
    30421 ctatttcctt gacagaataa ggtttttata tttcagtttt tttcactaaa aatcgtgaaa
    30481 cacgtcaata agtttgatga acaaattaat cattatggta gaagtcgatt gatttatttt
    30541 aatgcaatct ctgtggaatt ttcgaaagat ttattttttt aatacagtct caatataact
    30601 attcaaacat aaaataacgt tatttgattt atttgtttat agtaaatttt atttagtaat
    30661 cattgcttta aaatactacg cttatataac agagaaaata agcgttataa taaagaacag
    30721 tattatttaa aatgacagaa aacattacta ttaaagaaat aaaaaacata aaaaatctta
    30781 agaaatcggt ttcatttagc ttttgagata aaataatagt acagttttat acgctgtttc
    30841 atatgtttag aaaatttgaa ttagatttga aaactctaat ttttaaatga taaaaaaagc
    30901 gagaatgctg gttttactgt atattaacct tagataactt tcattttttt aaaaaaatta
    30961 tgaaaaaaag gtagctattt aaaagttgat tatgtataat agaaacagat gggagattat
    31021 tatgatcttc tgttacaaaa aagtgattct tatcacgaaa aggagtggtg cacgttgtta
    31081 acattatata cttccccaag ttgtacttcc tgcagaaagg ctcgtgcgtg gttgcaagaa
    31141 catcagattc catttgttga acgtaatatt ttttcagaac cattgaacag ttctgaatta
    31201 aaagcgattt tacaaatgac agaagacgga acagaagaaa tcatctcgac acgttccaaa
    31261 gtattccaaa aactgaacat ggatttagat gatctgccgt tacaagaatt attggaactt
    31321 gtgcaaaaca atcctggatt gcttcgccgt ccaatcatga ttgatgataa acgtctgcaa
    31381 gttggtttta atgaagatga aattcgtcgg ttcctaccgc gcgatgtacg acaattagaa
    31441 ttacgtcaag cacaattgat ggctggatta tagaaggata atacgaaact tggaaaagac
    31501 tattacaaag atagtttttc caagtttttt tgtttttaca aaaagtattc ttgattagat
    31561 gtataaaaag gtagtagaag atgatgattg cacgaaaaat agagagggaa aattagctga
    31621 aaagtcggct atatagtatt cgtctgccaa aaatgttata gttaaataag atagcttgaa
    31681 aggttgaaaa atccgctggt tttattaaat tcgcttaaca gcgagatttt ttttctttac
    31741 atttaagctg atttcgctac aatgaatcta tattaaacat tgtggcaaag tgaggtgtag
    31801 caaaatggaa atggaacata ttaatgaaaa tacgatccgt gtactaattg gtcatgaaga
    31861 tttagaggaa cggggcgttt catttttgga tttacttggc aatcacaaag aaatcgaaaa
    31921 tttcttttat agtattttgg aagaagttga tatcgaagag gagttccgag gaagcgaagc
    31981 tgtcactttc caagttttgc caaaaggaga cggattagaa ctgtttatca gtaaaaatct
    32041 tccggatgaa gaaatttctc aatcagaaga ttctactgtt gattcagaag atgtgagtga
    32101 ctttcttcgt cagcagatta ttggcagcga tccggaggaa gaattatctg gaagcgccct
    32161 agaagaacag gaaagacaag caacttttgc ttttgaagat tttgaacaag tgatccaact
    32221 tgcaacagat gtcgaactag agtctgcttg gactgatttg tatttattcg aagagcgcta
    32281 ttacctagtc gttcacttct ggatggaaaa tctcaaccaa gcggatgtag agaatcagat
    32341 tgctcgaatt ttggagtttg ctgaaaagag cgatcgtaca ccagaaacat tgaatgaata
    32401 tggacaatgt ctgatggcgc gtaatgctat cgagcgaacg aaattttatt ttgtataaat
    32461 cagcattata gtaagagact gtgacaaaaa ttgtgtcgca gtcttttttt cgtataaatg
    32521 tgttcaaaag cttgcatctg cggtaacaag cccaaccaaa cgaaaaatat tgggaactat
    32581 ttttgtttag ctgttcttat tacttgatgc agaatagctt tttcacgccc tcttttttcg
    32641 tgtaaataaa ttcttagacg taaataagtt cagagggaga aaaatgctgc ttgcaatgaa
    32701 tgaatataat aagtgctgtt ttgctaaaca tgcagaaaaa ggaaagaaat attattgtcg
    32761 tggctgtcga aaaagagtga ttttgaaaaa gggaaagaaa aaatgtgctc attttgccca
    32821 tcaaaaatca gataactgtt cggtctttag tgaaagagaa tcaaaagagc acttacagtt
    32881 aaaggaatgc ttcatggatt ggctgggaca gtcagtagaa ccagtatttt tggaagccta
    32941 tttgccgagg ttgcggcaaa ggccggatat tttgttggcg aatctagcaa tagaaatcca
    33001 atgttctaga ctgtctcacc agagatttat tgaaagaacc caaaattatt tgaataacag
    33061 ttaccaagta tggtggatac ttggtaattc ttttcttgga caatcgcaat tctcattgat
    33121 agaaaaaagt tgctgctact ataatagaaa aagaggagtg cattgctgga aagctgatct
    33181 caagaaacaa aagctttatt tgtatcatca tattacagag actgtatcag ggcatatttc
    33241 cttttgcagt agctgctgga ctttttctag tagggacctt aaagaaatat tcactagtaa
    33301 cgaaattaaa atgaaccaaa tgaaaaaaat agaaaaagtg agtgaagatg ggaagaaatg
    33361 gctgacacgc caacttattc acaagcaaaa gaatacagta tccatacaag aacaatgcta
    33421 cttgcgtcat aaacatcttt tgcatctatc atcgtggatc tatcaaaaaa gtcggttttt
    33481 cttttattta caagaacagg tttttcttta tcgaatgttg tacgaagaag ccttggaaaa
    33541 ccagaaagtt cctgatttca actcatggct ttgccaaata aaagaacata aaagggaatg
    33601 gctttttcct atgattgatg aagagatggt ctatcagcag ttttttaacg aatgcataca
    33661 cttgtcttct ttgaaaaaat agtaagtata aaaataggct aggaatcggt tttctatgta
    33721 aaacaagatt tagttatgct aaaattgatg gaagcagaca catgaatgag tagaaaagtt
    33781 agatgctgtg acatgtggat gttagactat gatctatttt gatagataga gctaagggaa
    33841 gaaaaggagt ggatttcatg gaagtaaagc agttgccaaa acgagaagaa ttgcctgaaa
    33901 atttaacttg ggacttgacc aagatctttt caagcgacca agagtttgat gagaaatatt
    33961 tggaattatc agaagagtta aaacaatctg aaaaacacaa aggaacactt gatcaaggcg
    34021 cttctcaatt tttaaatgcg attgaattcg tattgagggt ttatcgccaa actgaagtca
    34081 tttatgtata tgcgcacctt aaaaacgatc aagacactgg aaatacagat taccaagcgc
    34141 tttatgcaag agcaagcagt ctgttttcga aagttagtga agccgtttcc tggtttgaac
    34201 cagaaatatt gcaattgtca gatgaccaga tttggcaata tttcaaagaa gaaccaaaat
    34261 tggaagtcta tcgccattat atccagcaaa tagtagataa tcgagcccat gtcttatctg
    34321 ctgagcagga atctcttctt gctggagcag gtgaaatctt tgatgcttca agtgatacat
    34381 ttgctgtttt gaataatgca gatctagttt ttccaacgat tgaaggagaa aatggtgaaa
    34441 tagtccaatt atctcatggc gtgtatggtc agttgctaga aagcacggat cgaagggtgc
    34501 gcgaagcagc atttaagggg ttgtacagtg tttacgaaca atttagaaat acatttgctt
    34561 ctactttagg cacacatata aaaggacata attttaaagc gaaagtccgt aattacagct
    34621 ctgccagaga agcgtctttg agcaataatc atattcctga aagtgtatac gatactttgg
    34681 tagacgtggt aaacaagcat ttgcctttgt tacatcgata catggaatta cggaaacgtt
    34741 tattagaagt ggaaaaactg cacatgtatg atctttatac accggtctta ggggaagctc
    34801 caattacctt tacgtacgaa gaagcaaaag aaaaagcttt agaagcactg aaaccaatgg
    34861 gtgaagaata catggccatc gtagaaaaag cattctctga acgttggatc gatgttgtcg
    34921 aaaataaagg gaaacgaagc ggtgcttatt cttcgggaag ctatgacaca aatccatata
    34981 ttttattgaa ttggcatgat acgctggatc agctatttac gcttgtccac gaaatgggac
    35041 atagtgttca tagttatttc actcgttcga accagcctta tgtgtacggc gactactcca
    35101 tctttttagc agaaattgct tcgacaacga atgaaaatat cctaacggag tatttattgg
    35161 aaacagaaaa agaccctcgt gtacgggctt atgtactcaa ccattacttg gatgggttta
    35221 aaggaacagt tttccgccag acacaattcg ctgaattcga gcatttcatg catacggaag
    35281 atgaaaaagg tgtgccattg actagtgaat acctaagtga tagttatggt aaattgaatg
    35341 caaaatatta tggtccagca gtcgaagaag acccagaaat taaattcgaa tggtcacgga
    35401 taccgcattt ttattataat tactatgttt tccaatactc gactggcttt tctgctgctt
    35461 cagcactggc gaagaaaata ctaaaccagg aaccagaggc actagagaac tacttggctt
    35521 acctaaaagc aggtaacagc gattatcctg tggaagtaat gaaaaaagca ggagttgata
    35581 tgacacaggc tgcatatatt gaagatgcaa tgtcgatgtt tgaacaacgt ctaaatgaat
    35641 tagaagaatt gatcgatcgt ttataaggct ttctattttt gattttctcg aatagaaacg
    35701 aaaaaaatac gtcggctctc ttgtaaggga gtcgacgtat tttttta
//
LOCUS       DAYYHS010000018        34962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000018 DAYYHS010000000
VERSION     DAYYHS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.018"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHA6A_000935"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHA6A_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8969157.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHA6A_000936"
     CDS             complement(2020..2922)
                     /locus_tag="ACHA6A_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8969158.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHA6A_000937"
     CDS             complement(2919..3611)
                     /locus_tag="ACHA6A_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8969159.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHA6A_000938"
     CDS             complement(3654..4295)
                     /locus_tag="ACHA6A_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8969160.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHA6A_000939"
     CDS             complement(4333..4647)
                     /locus_tag="ACHA6A_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969161.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHA6A_000940"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHA6A_000940"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8969162.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHA6A_000941"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHA6A_000941"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8969163.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHA6A_000942"
     CDS             complement(5984..6916)
                     /locus_tag="ACHA6A_000942"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8969164.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHA6A_000943"
     CDS             complement(6913..7689)
                     /locus_tag="ACHA6A_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8969165.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHA6A_000944"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHA6A_000944"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8969166.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKVIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHA6A_000945"
     CDS             complement(10933..12009)
                     /locus_tag="ACHA6A_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8969167.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHA6A_000946"
     CDS             complement(12014..13294)
                     /locus_tag="ACHA6A_000946"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8969168.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHA6A_000947"
     CDS             complement(13313..14239)
                     /locus_tag="ACHA6A_000947"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8969169.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHA6A_000948"
     CDS             complement(14252..15535)
                     /locus_tag="ACHA6A_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8969170.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHA6A_000949"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHA6A_000949"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8969171.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHA6A_000950"
     CDS             complement(16323..17234)
                     /locus_tag="ACHA6A_000950"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8969172.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHA6A_000951"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHA6A_000951"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8969173.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHA6A_000952"
     CDS             complement(17700..18398)
                     /locus_tag="ACHA6A_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8969174.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHA6A_000953"
     CDS             complement(18385..18771)
                     /locus_tag="ACHA6A_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969175.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHA6A_000954"
     CDS             complement(18918..20588)
                     /locus_tag="ACHA6A_000954"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8969176.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHA6A_000955"
     CDS             20771..21004
                     /locus_tag="ACHA6A_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8969177.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHA6A_000956"
     CDS             complement(21103..21534)
                     /locus_tag="ACHA6A_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8969178.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHA6A_000957"
     CDS             21613..22026
                     /locus_tag="ACHA6A_000957"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8969179.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHA6A_000958"
     CDS             complement(22064..23212)
                     /locus_tag="ACHA6A_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8969180.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHA6A_000959"
     CDS             complement(23334..23930)
                     /locus_tag="ACHA6A_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8969181.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHA6A_000960"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHA6A_000960"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8969182.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRTVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHA6A_000961"
     CDS             27160..27591
                     /locus_tag="ACHA6A_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8969183.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHA6A_000962"
     CDS             complement(27636..28985)
                     /locus_tag="ACHA6A_000962"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8969184.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHA6A_000963"
     CDS             complement(29055..30218)
                     /locus_tag="ACHA6A_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8969185.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHA6A_000964"
     CDS             30347..30670
                     /locus_tag="ACHA6A_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969186.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHA6A_000965"
     CDS             complement(30853..32202)
                     /locus_tag="ACHA6A_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8969187.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHA6A_000966"
     CDS             complement(32271..33224)
                     /locus_tag="ACHA6A_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8969188.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHA6A_000967"
     CDS             complement(33326..34516)
                     /locus_tag="ACHA6A_000967"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8969189.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34962)
                     /locus_tag="ACHA6A_000968"
     CDS             complement(34716..>34962)
                     /locus_tag="ACHA6A_000968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969190.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9953 a         7640 c         6119 g        11250 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggatta ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagtacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DAYYHS010000019        34662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000019 DAYYHS010000000
VERSION     DAYYHS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.019"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHA6A_000969"
     CDS             591..1280
                     /locus_tag="ACHA6A_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8969191.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHA6A_000970"
     CDS             1267..2475
                     /locus_tag="ACHA6A_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8969192.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHA6A_000971"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHA6A_000971"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8969193.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHA6A_000972"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHA6A_000972"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8969194.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHA6A_000973"
     CDS             complement(6578..7273)
                     /locus_tag="ACHA6A_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8969195.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHA6A_000974"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHA6A_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8969196.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHA6A_000975"
     CDS             complement(8472..8663)
                     /locus_tag="ACHA6A_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8969197.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHA6A_000976"
     CDS             complement(8684..9574)
                     /locus_tag="ACHA6A_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8969198.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHA6A_000977"
     CDS             complement(9561..10328)
                     /locus_tag="ACHA6A_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8969199.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHA6A_000978"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHA6A_000978"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8969200.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHA6A_000979"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHA6A_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8969201.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHA6A_000980"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHA6A_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8969202.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHA6A_000981"
     CDS             14899..15282
                     /locus_tag="ACHA6A_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969203.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHA6A_000982"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHA6A_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8969204.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHA6A_000983"
     CDS             complement(16226..17044)
                     /locus_tag="ACHA6A_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8969205.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHA6A_000984"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHA6A_000984"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8969206.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHA6A_000985"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHA6A_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8969207.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHA6A_000986"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHA6A_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8969208.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHA6A_000987"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHA6A_000987"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8969209.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHA6A_000988"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHA6A_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8969210.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACHA6A_000989"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACHA6A_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8969211.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHA6A_000990"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHA6A_000990"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8969212.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHA6A_000991"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHA6A_000991"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8969213.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHA6A_000992"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHA6A_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8969214.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACHA6A_000993"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACHA6A_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8969215.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHA6A_000994"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHA6A_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8969216.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACHA6A_000995"
     CDS             28352..30325
                     /locus_tag="ACHA6A_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8969217.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACHA6A_000996"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACHA6A_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8969218.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHA6A_000997"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHA6A_000997"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8969219.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACHA6A_000998"
     CDS             33204..34496
                     /locus_tag="ACHA6A_000998"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8969220.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10565 a         6734 c         6854 g        10509 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aa
//
LOCUS       DAYYHS010000020        34406 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000020 DAYYHS010000000
VERSION     DAYYHS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.020"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..990)
                     /locus_tag="ACHA6A_000999"
     CDS             complement(253..990)
                     /locus_tag="ACHA6A_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8969221.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(992..2494)
                     /locus_tag="ACHA6A_001000"
     CDS             complement(992..2494)
                     /locus_tag="ACHA6A_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8969222.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2753..4747
                     /gene="uvrB"
                     /locus_tag="ACHA6A_001001"
     CDS             2753..4747
                     /gene="uvrB"
                     /locus_tag="ACHA6A_001001"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8969223.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            4757..7573
                     /gene="uvrA"
                     /locus_tag="ACHA6A_001002"
     CDS             4757..7573
                     /gene="uvrA"
                     /locus_tag="ACHA6A_001002"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8969224.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     MTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            7836..9101
                     /locus_tag="ACHA6A_001003"
     CDS             7836..9101
                     /locus_tag="ACHA6A_001003"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8969225.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9105..9836
                     /locus_tag="ACHA6A_001004"
     CDS             9105..9836
                     /locus_tag="ACHA6A_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8969226.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            9971..10855
                     /gene="rapZ"
                     /locus_tag="ACHA6A_001005"
     CDS             9971..10855
                     /gene="rapZ"
                     /locus_tag="ACHA6A_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8969227.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            10852..11850
                     /gene="yvcK"
                     /locus_tag="ACHA6A_001006"
     CDS             10852..11850
                     /gene="yvcK"
                     /locus_tag="ACHA6A_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8969228.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            11884..12819
                     /gene="whiA"
                     /locus_tag="ACHA6A_001007"
     CDS             11884..12819
                     /gene="whiA"
                     /locus_tag="ACHA6A_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8969229.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            13387..14043
                     /locus_tag="ACHA6A_001008"
     CDS             13387..14043
                     /locus_tag="ACHA6A_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969230.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            14222..14787
                     /locus_tag="ACHA6A_001009"
                     /pseudo
     CDS             14222..14787
                     /locus_tag="ACHA6A_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15486..16007)
                     /locus_tag="ACHA6A_001010"
     CDS             complement(15486..16007)
                     /locus_tag="ACHA6A_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8969231.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            16205..19885
                     /gene="nifJ"
                     /locus_tag="ACHA6A_001011"
     CDS             16205..19885
                     /gene="nifJ"
                     /locus_tag="ACHA6A_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8969232.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            20074..20961
                     /gene="cdaA"
                     /locus_tag="ACHA6A_001012"
     CDS             20074..20961
                     /gene="cdaA"
                     /locus_tag="ACHA6A_001012"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8969233.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            20958..22127
                     /locus_tag="ACHA6A_001013"
     CDS             20958..22127
                     /locus_tag="ACHA6A_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8969234.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            22151..23506
                     /gene="glmM"
                     /locus_tag="ACHA6A_001014"
     CDS             22151..23506
                     /gene="glmM"
                     /locus_tag="ACHA6A_001014"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8969235.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(23578..25383)
                     /gene="glmS"
                     /locus_tag="ACHA6A_001015"
     CDS             complement(23578..25383)
                     /gene="glmS"
                     /locus_tag="ACHA6A_001015"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8969236.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            25793..26083
                     /locus_tag="ACHA6A_001016"
     CDS             25793..26083
                     /locus_tag="ACHA6A_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969237.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            26096..27121
                     /gene="sppA"
                     /locus_tag="ACHA6A_001017"
     CDS             26096..27121
                     /gene="sppA"
                     /locus_tag="ACHA6A_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8969238.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            27124..27783
                     /locus_tag="ACHA6A_001018"
     CDS             27124..27783
                     /locus_tag="ACHA6A_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8969239.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            27940..28872
                     /locus_tag="ACHA6A_001019"
     CDS             27940..28872
                     /locus_tag="ACHA6A_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969240.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            29026..30315
                     /gene="tig"
                     /locus_tag="ACHA6A_001020"
     CDS             29026..30315
                     /gene="tig"
                     /locus_tag="ACHA6A_001020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8969241.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            30562..31812
                     /gene="clpX"
                     /locus_tag="ACHA6A_001021"
     CDS             30562..31812
                     /gene="clpX"
                     /locus_tag="ACHA6A_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8969242.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            31895..32482
                     /gene="yihA"
                     /locus_tag="ACHA6A_001022"
     CDS             31895..32482
                     /gene="yihA"
                     /locus_tag="ACHA6A_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8969243.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(32556..32711)
                     /locus_tag="ACHA6A_001023"
     CDS             complement(32556..32711)
                     /locus_tag="ACHA6A_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8969244.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            32847..34175
                     /locus_tag="ACHA6A_001024"
     CDS             32847..34175
                     /locus_tag="ACHA6A_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969245.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            34186..34395
                     /locus_tag="ACHA6A_001025"
     CDS             34186..34395
                     /locus_tag="ACHA6A_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969246.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT        11238 a         5947 c         7439 g         9782 t
ORIGIN      
        1 gtagttgtca tgtttgtacc aacaaaaaag ccagctttag aataagataa tcatcatagt
       61 ttctgaaggt ggtatcgact atgtacttga tatcgtcaat gaaccgatgg tactttctta
      121 tgcgatggaa caggtagagc gattgcttgt ttgtgcagga gaacaagcga tgcggctggt
      181 acagttaagt gaaaaaacaa gaaactaaaa atgcccggtc gtcatgaagc gaccgggcat
      241 ttttggtttt tattaaatat ttaatacttt attcaagaaa tcttttgtcc gttcattttg
      301 cggattttca aagatttgtt gaggcgttcc gtcttctaaa aagtttccgt cgtcaatgaa
      361 catgacacga ttggccactt ctttagcgaa gcccatttcg tgcgtaacga taaccatcgt
      421 cataccttgt tctgctaact ttttcatgac gttcaagaca tcaccgacca tttcggggtc
      481 gagtgcagat gtaggctcat caaaaagcat gatgtcaggg ttcatcgcta gagcacgagc
      541 aatagccaca cgctgctttt gtccaccgga aagcatttca ggatacatat cttttttatc
      601 ttctagacca actgtctgca gcaatttgat tgcttttttc tctgcttctt ctctagattc
      661 tttcttcagg tccatcggtg ctaaaatgat gttttccaaa atagaaagat gagggaatag
      721 gttaaagtgc tggaatacca ttccgatatg ttgtctcacc aagttgatat ctgtgctttt
      781 atccatcaag tgggcgccgt caataataat ttcaccgtca gaaggttctt ctaaacgatt
      841 caagcaacgc aaaaatgtac tttttcctga acctgaaggg ccaatgatac agacgacatc
      901 tccttcgttg atggacacat tgatgtcgtt taatactgtg ttatcgccgt attttttgac
      961 taaatgctca actaagattt tttctgccat cttacttcac cttcttttct aaaacttttg
     1021 ctaattttgt caatgctgtg atcaaaatca agtacatgat cgcaatgatc aagtaaacat
     1081 acgtactttg tgttgtacgt gcaacaatga tcttaccagt ttgcagcaat tcgactacac
     1141 cgattgcgga aatgatcgtt gtgtctttta atgagataac gaactgattg acgaatgatg
     1201 ggatcatgat gcgtatcgct tgcggcaaaa tgattttttg cattgtccga ttataagaaa
     1261 gacctaaact tctggaagct tccatttgtc cgacaggaac ggcgttgatc ccgcctcgaa
     1321 caatctctgc gatataagca cttgcgttaa gagtcaaggt gataacacca gcaaggaagt
     1381 caggaatcgt aaatccaacg ataccaggca acccaaagaa aataaagaac gcaagaacca
     1441 tcattggaat tcctcggatg atatcgacat aaatactagc aattgtgcga agagcgcgta
     1501 ttggtgctac actgaataat ccaaaaatca caccgattac tatagccaaa gcaaaggaga
     1561 tcaaagttag gagaattgtt ctccaaagtc ctttcaacaa tgcttgatag ttgttttgga
     1621 taagtccaag aaatgttgat tcatcagcag tggaagactc actggaagtt tctgaaccat
     1681 ccgctacata ttctttaata atcttatcgt attcgcctgt tctttttaat tctttcaatc
     1741 cttcattgaa catattaaga agctcaggat tttgtccttt tttaacggca aatgcatatt
     1801 ttccgccaga ttcttttttg atcggtgttt ccaattcttg tccttgtttg atcgcataac
     1861 cgattaccgg ataatcatcc atcattgcat cgattgcgcc aacttttaac ccgtcgtaca
     1921 attgatcagc agcatcaaaa tatttgatag aatagccata ttcagattca tggtcgctta
     1981 aaaattcagc actttcggta ccaacttttg ctccgactgt ttttcctttc aaatcttcat
     2041 aagacttgat cgaagtatct ccttttttga cagctaattg gatcccgcta tcaaagtaag
     2101 aatcagaaaa atcatagctt ttctttcgtt catctgttac agtcattccg gcaatcattc
     2161 cgtctgcttg tcctgattcc aaagcttgca aagcagcaga gaaaccacgg tagtccattc
     2221 ggatgttaaa tccttgcagt ttggcgatgt tttccatcaa ttttacatcg atgccttcgt
     2281 actcgccctt tgcgttttga aattcaaatg gagcaaaagc agagtcactg gcaattacgt
     2341 atacgtcttt tttgggctct attttttgca ttgtctcgct tgtgcttgac tctgccgttg
     2401 tagtgttgtc agcttgtgca acatgtccaa acgcaagtgt cacaagtcct gtcattaaga
     2461 aaacaaatga gtgaaataaa tgctttcttt tcataatcaa cctcctaaat ttccaatatt
     2521 ttattattat aggcgaaaag tgctgaatca gacaagactg taagctcgaa aaaatgaaaa
     2581 aaatgcgagt ttctaacatt ttatgttata aaacgtaaca tgcataataa gataaaaaag
     2641 tggcgaaagt atgttcggtg tggtataatt ttttagtaag gaatgccttt gctttccttt
     2701 tctttttatt ggtcgtaaaa tgaaaaggaa tgaatgaaaa ggaggacgaa gaatgattga
     2761 gagagatacc tcccgacact ttgagcttgt ctcgaaatac caaccggctg gtgatcagcc
     2821 tgaagcgatt aaccaattag tggatggcgt agtcggcggg aaaaaagcac aaatcttatt
     2881 aggtgcgact ggaacaggga aaacatatac gatttccaat cttattgaga aagtgaataa
     2941 accaacacta attattgccc ataacaaaac attggctggt cagctgtacg gggaattcaa
     3001 ggaatttttc cctaacaatg ctgttgaata ttttgtttct tattatgatt attatcagcc
     3061 tgaagcttat gtgccttcaa gcgatacgta tatcgaaaaa gattcaagtg taaatgatga
     3121 gatcgacaaa ttacgacatt cggctactag ttcattatta gaaagaaatg atgtgatcgt
     3181 cattgcatcc gtttcatgta tttttggatt aggttcgcct tttgagtacc aaaaacaagt
     3241 agtttcgatt cgacagggag cagaactaga tcgcaatcag ttgattcgtg acttagtcag
     3301 tatccaattc gaaagaaatg atatagattt tcagcgcggt cgttttcgag tgcgcggtga
     3361 tgtcgtggag atttttccgg cttcaagaga tgaacgtgcc ttacgtgtgg agttttttgg
     3421 agatgaggtc gaacgtatcc gagaagtcaa tgcactgaca ggtgaagttt taggagaaac
     3481 ggaacacgtg gcaatcttcc cggcgactca ctttgtgacg aatgatgaac atatggagca
     3541 tgccgtagcg aatatcaaag cggaattaga acagcgttta actgtcttgc gaaatgaaaa
     3601 caaacttctg gaagctcaac gtttagaaca gcgaaccaat tacgatattg aaatgatgtt
     3661 agaaatgggc tacacgtcgg gaattgaaaa ttattctcgg catatggacg gacgtaaaga
     3721 aggtgaaccg ccttatacgc tattggattt ctttcctgaa gattttttga tcgtggcgga
     3781 cgaatcgcac gtaaccatgc ctcaagtcag aggaatgtat aatggtgacc gagcgagaaa
     3841 acagatgcta gtagattacg gtttccgttt gccatctgct ttagacaatc ggccacttcg
     3901 gttagaagag tttgaaaaac atgtcaatca gatcatttat gtctctgcga cgccaggacc
     3961 atatgaacat gaacaaacag ataccgttat ccaacaaatc atccgtccga cagggctgct
     4021 tgacccagtg atcgaagttc gtccaatcat gggacaaatc gatgatctag taggcgaaat
     4081 aaatgaacga gtcgaaaaag accagcgggt gtttgtaacg acattgacga aaaaaatggc
     4141 agaagacctc acagattatt tcaaagaatt agggatcaaa gtcaaatatt tgcatagtga
     4201 tatcaaaacg ctggaacgaa cagaaatcat tcgagatctt cgattaggtg aatttgatgt
     4261 gttagttgga atcaatttgc ttcgtgaagg attagacgta cctgaagttt cccttgttgc
     4321 tatcttggat gcggataaag aaggcttttt acgtagtgaa cgttccttag tccaaacgat
     4381 tggacgagct gctcggaatg aagaaggtaa agtaatcatg tacgcagaca aagtaacaga
     4441 ttctatgcgt ctagcgatgg atgaaacatc aagacgccgt acgattcagc agaagtacaa
     4501 tgaagaacat gggatcgttc caaaaacgat cattaaagaa atccgagatt tgatttctat
     4561 tacgaaagaa tcggaagatg atacaaaaga agcggtacaa gtctcgtatg aagaaatgac
     4621 gaaagaagaa aaagatacgt tattgatgaa attagaaaaa gaaatgaaaa atgcggcgaa
     4681 agcattggat tttgaaacag ccgctaatgt gcgagatatg attttagaat taaaagcttc
     4741 aaagtaggag gacaacatgg ctaacgataa aattacgatt cacggtgcac gtgcccataa
     4801 cttgaaaaat atagatgtaa cgattccacg agataaattc gtggtcgtta caggtttgtc
     4861 cggttctgga aaaagttccc tagcatttga tacgctgtac gctgaagggc agcggagata
     4921 cgtagagagc ctttctgctt atgctcgtca gtttttagga caaatggata agccggacgt
     4981 agatagcatc gacggattga gtccggcgat ttctattgat cagaaaacaa caagtaaaaa
     5041 tcctcggtcg acagtaggta ctgtgacgga aatcaatgac tatttacgct tgttgtttgc
     5101 acgtgtagga catccgattt gtcccaacga tcatatcgag attaccagtc aatcagtaga
     5161 tcaaatggca gatcaagtaa tggctttgcc tgagcgcaca aagatccaag tgttggcacc
     5221 gatcgtggta aagaaaaaag gacaacataa aaaaatattc gaacgaatcc aaaaagaggg
     5281 atacgtccgc gttcgagtcg atggtgagat ctatgattta agtgaagtgc ctgagctgga
     5341 gaaaaacaaa aaacatgata tcgcgatcgt catcgaccgg atcattgtga aagaagggat
     5401 tcgttctcgt ctatttgatt ctttagaggc ggctctgcga ttggcagacg gctatgctct
     5461 ggttgacgtg atcggtgaag aagaaatgtt gtttagcgaa cactatgcct gtccttattg
     5521 cggatttact gttggcgaac tagaaccaag actgttctca ttcaatgcgc cttttggagc
     5581 ttgtccagac tgtgatggtt taggtgtcaa attagaagtc gatcaagatt tagtgattcc
     5641 agatagaacg aagacactta gtcaaggtgc tatcgttcca tggaatccaa tcagctctca
     5701 gtattatcct caaatgttag cgcaggcttg tgaaagtttc ggtatcgatt tagatacacc
     5761 attcgaagaa ttaccggaag agcatcagga tatcatcttg aatgggacag atgatctttt
     5821 ccatttccat tatgaaaatg attttggtgg cgttcgcgat gtagaaacga ctttcgaagg
     5881 ggtattgaag aatatcgagc gtcgttatca tgatacgaac agtgatttca cgcgtgagca
     5941 aatgcgtctt tatatgacag aattaacttg tcagacatgt aaaggttatc gattgaatcc
     6001 ccaagctcta tctgttcaaa tcaatggaac gaatatcggg caaacaaatg agttgtctat
     6061 tcaaaaagca gtagatttct tctcaaactt aacgttatct gaacaagaga aagtaattgc
     6121 caaaccgatt ttaaaagaag taaatgacag actttcattc ttggaaaatg tgggactaga
     6181 ctatctaact ttgagtcgcg cttccggaac attatctggg ggagaagcgc agcgaatcag
     6241 attggctaca caaattggat cgaatctttc tggagttttg tatattttag atgaaccatc
     6301 gatcggattg catcaacgcg acaacgatcg actgattggc tcattaaaga aaatgcgtga
     6361 tcttggcaac accctcgttg tagtcgaaca tgatgaagat acgatgtggg cagcagatta
     6421 tctgattgac gtcggacctg gggccggaga acaaggcgga caaatcgtgg ctgcaggcac
     6481 tcctgaagaa gtagctaatg atgaaaattc tttgacaggg aaatatcttt cagggaaaaa
     6541 atcaatccct gtaccaaaag aacgccgtaa aggtaatggg aaagcaatca agatcactgg
     6601 tgcttcagaa aacaatctga aaaatattag tgttgaattt ccattaggag aatttgtagc
     6661 agtcacaggt gtgtctggtt ctggaaagag tacattggtc aatagtatct taaagaaatc
     6721 gttagcgcaa aaattaaata agaattctgc taagccaggt aaattcaaga caatttccgg
     6781 ctacgaaagt atcgaaaaga tcatcgatat cgatcaaagc ccaatcggcc ggacgccgag
     6841 aagtaatcca gcgacttata caagtgtatt tgatgatatc cgtgggttat ttgctcaaac
     6901 gaacgaggca aaaatgcggg gttataagaa agggcgtttt agtttcaacg taaaaggcgg
     6961 tcgttgtgaa gcttgtcgcg gggatggaat tattaagatc gaaatgcact ttttgcctga
     7021 tgtctatgtt ccttgtgaag tatgtcatgg caaacgatat aactctgaaa cattagaagt
     7081 gcattacaaa ggaaaaagca ttgctgatat tttggaaatg acagtagaag atgctgtaga
     7141 attcttcaag cacattccaa agattcatcg caaactgcaa acgattgttg atgttggctt
     7201 aggttatgtg acgatggggc aaccagcaac gacattgtcc ggtggtgagg cacaacggat
     7261 gaaacttgcc agtgaattgc acaaaatctc taatggaaag aatttctata tactagatga
     7321 accaatgaca ggacttcata gcgatgacat cgcccgcttg ttgcatgtat tacaaagatt
     7381 agtagatgct ggtaacacag ttttagtgat tgaacacaat ctagatgtaa tcaaaacagc
     7441 agattatatc attgatttag gaccagaagg tggagaaggt ggaggaacga tccttgcgac
     7501 tggaacacca gaagaaatca ttaacgtaaa agaaagttat acaggtcact atttgaaaaa
     7561 aataatggta taaagaggat agatagctga gatagttaaa aaaacataaa aaaatgaatt
     7621 tttgcttgtt ttattaatat cagtgttcta gaatcattac atggtagttc tttctacact
     7681 gattggtata gacaaaaact tcctataagt tcaatccgtt caaaaaagaa ttgagcaatc
     7741 ggaggagttt cctttgattg cttttttctt tatggtatga tattccttgt gtagttggaa
     7801 aagaaaaaca agagattatg agtaagtgag agataatgat gaacagtatt gaaaatgaag
     7861 aatttatccc tatattatta ggaagcgaca tgaacgttta tggtatggca cgttctttta
     7921 atgaagcata cggcaaaatc tgccaagcat atgcttcgga tcagttagcc cctacccgct
     7981 acagtaaaat tgtcaatgta gaagttattc caggatttga taaagaccct gtgtttatcg
     8041 agacgatgct tcgtttggca aaagaaagat attctgataa gagtaaaaaa tatcttttga
     8101 ttgcttgtgg tgatggatat gcagaattga tctcccaaca taaacaagag ctgtcagaat
     8161 attttatctg tccatatatt gattattctt tatttgaaag actgatcaat aaagtaagct
     8221 tttatgaagt ttgtgaagaa tatgatcttc cgtatcctaa aacattgatc gtcagagaag
     8281 aaatgttagt aaatggacat ttggaacagg aattgccttt tgaattccca gttgctttga
     8341 aacctgcaaa cagtgtggag tatctttctg tccaatttga aggaagaaaa aaagctttta
     8401 ttttagagac tagagaagag ttcgatttaa ttttaggccg gatatacgaa gccggttata
     8461 aaagtgaaat gattgtacaa gattttatcc cgggtgatga tagtaatatg cgtgtgctaa
     8521 atgcgtatgt agatgaagat catcaagttc ggatgatgtg tcttggtcat ccactgttag
     8581 aagatcctac accagcatcg attggtaatt acgttgtgat catgccagat tataacgaaa
     8641 aaatctatca aaccattaag gcatttttgg aaaaaatcga atatacaggc tttgccaatt
     8701 tcgatatgaa gtatgatcct agagatggag aatataaatt atttgaaatc aatttgagac
     8761 aaggtcgtag cagtttcttt gtgacattga acggcttgaa tctggctcgt ttcgtcacag
     8821 aagatcgagt gtttaacaaa ccctttgttg aaacgaccta tggaacgaat caatcagata
     8881 aagcaagatt atggatgggt gtgcccaaaa aaatcttttt agaatatgct cgggaaaatg
     8941 aagacaaaaa actagctgag caaatgatca aagaaaatcg ttacggtact actgtgtttt
     9001 atgaaaaaga ccggtcaatc aagcgctggt tgttgatgaa atatatgttc cataactata
     9061 ttccaagatt caaaaagtat tttcatgtga aagaaggcta gttcatggag aattttttca
     9121 gtattttagg cggaatgggc acgatggcga cagaaagttt tgttcgcttg atcaaccacc
     9181 gaacgaaagc tacaaaagat caagaatatt tgaattatgt cttattcaat catgcaacag
     9241 ttcctgatcg tacagcttat attttagatc gatcagaaga aaatccaatg ccgtttctgc
     9301 tggatgatat tgagaaacaa aatttattgc agccgaactt cattgtgttg acttgtaaca
     9361 ccgcacacta cttttttgaa gaattacaag cggcaacgga tattcctatc cttcatatgc
     9421 caagagaagc agcaaatgaa cttgtccgtc agcatacgac agggagagta gcgattctag
     9481 gaacagaagg aagtatgaaa gctggaatct atgaacgcga agtcaaaaat cttggattcg
     9541 aaacagtgat tcctgatact gttcttcaag aaaaaatcaa ttatctgatc tatcatgaga
     9601 tcaaggaatc ggattatttg aatcaagagc tatattacga aatcttagaa gaagcggtag
     9661 agcgcctaaa ctgtgaaaaa gttatcttag gttgcacaga attgtcattg atgcatgaat
     9721 ttgctgaaga taaccattat ccagtgatcg atgcccaatc tattttagca gatcggacga
     9781 ttgaacgagc gctagctgaa agaagcaagg cattggatac agcatcggaa aagtaaaaaa
     9841 attggatttg gggcaaatat cctaaaagat atttggtcca aatctttttg taaaagaaaa
     9901 agaaaactgg taatgatacg cccggaatga tataatttta ctatgcacaa tgtgagagga
     9961 gttcgtaatt atgacagata atttgcaatt agtcatcata acaggtatga gcggtgcagg
    10021 gaaaacagta gctatccaaa gttttgaaga tatgggctat ttttgtatcg ataatatgcc
    10081 gcctcgattg attccaaaat tctgggaatt aatcaaagaa tcaggcaaag tcacaaaaat
    10141 ggctttagga gtggatttac gttctcgttc cttctttgaa gaaatccaaa acatgctgat
    10201 cgaaatcgaa aatacgaact tcatcgacac ttctatctta tttttagatg cttcagacga
    10261 agaactagtg gctcgctaca aagaaacaag acgagcacat ccaatggcga tggacggttt
    10321 gatcactgaa gggatacgta aagaacgggc gatactagac gacttgaaag cccaagcttc
    10381 cgtgatcatt gatacgacga cattgacacc acgacaatta cgtgaaaaaa tcaatgagtc
    10441 attcaaatca aagggagacc aaggatttcg gatcgaattt gtatcatttg gttttaaata
    10501 cgggttgccg atcgattcgg atattgtgat ggatgtgcgt tttttgccca accctcatta
    10561 tatcccggaa ctaagaccat tgaccggaaa agatcagcca gtctatgatt atgtgatgtc
    10621 ctttgatgaa acgcaatcat tctaccataa atttttggat cttctggaaa ctgtattgcc
    10681 tggatacatt aaagaaggaa aaagcagctt gacagttgca atcggctgta caggcggtca
    10741 acaccgttct gtggcattga ctgaacgttt ggcaaatgct ttagggaaaa actataaagt
    10801 caatgtgaca catcgggaca aggataaaag gaaagagacg gtgaaccggt cgtgaaaatg
    10861 aaaacctacc ggataagaaa accaaagatc gtagtggtcg gcggtggcac tggtctgcca
    10921 gttatcctga aaagtttgcg aaatcaagga gcagatatca cagcagtagt gactgttgca
    10981 gatgatgggg gaagcagcgg tgcaatcaga gaatcgatcg ctatggctcc tcctggtgac
    11041 ttgagaaatg tcttagtggc gttatcggat atgccacaat tctatgaaga tattttccaa
    11101 taccgtttca aaaaggaaga tcagttttta gccaatcata cgatcggtaa tctgatcatt
    11161 gccgcagttt ccgagatgcg gggaagcact tatgaggcga ttcaattatt agcaaaaatg
    11221 atgcatgtag atggacatgt ttatccttct tcagaaactc ctttgacctt gcatgctgtc
    11281 tttaaagacg gaacgactgc agtaggagaa tctaaaatcg cgatcgaccg taaaaccatt
    11341 gaccgtgtgt atgtctgcaa cacaaatgat aaaagtgaag ccaaagcggc tcgaaaagtc
    11401 gtttcagcaa ttatggacgc cgacatggtc gttttgggac ccggaagttt attcaccagt
    11461 atccttccaa atttagtgat accagaaatc ggcgaagcaa tgctcaagac aactgctgaa
    11521 accgtctata tctgtaatat catgacccaa aaaggggaaa cggaacattt cacagatgcc
    11581 gatcatatac gtgttttaca caagcatttg aatcagcctt ttatcgatac agtattggtc
    11641 aatacggaaa aagtgcctga agattatatg gacccagaga tctatgatga gtatctagta
    11701 caagtcgcac acgacttcaa cggattgaga aatgaaggat gtcgagtgat ttccaccgac
    11761 tttttggaat tgaaagatgg tggtgtcttc cacgatggag acaaagtagt agaagaattg
    11821 ttccgtttag ttttcggttc aaaatattaa gcaatttatt gttggagaaa ggaggagaat
    11881 aggatgtcat ttgctgcaga tgtgaagaaa gaactaactg gtttggaagt tcatcgagaa
    11941 catgcgaaag cggaattagc tgctttgctg cgaatgaacg gctctttagg tattgtgaac
    12001 cagcagttta ttttgaacgt ccaaacagaa aatgctgcaa ttgccagacg tatctattct
    12061 cttttgaaag atcattatca ggtacgaagt gagctactcg ttcgtaaaaa aatgaaatta
    12121 aaaaagaaca atgtctatat cgtccggttg aagcaaggca caaaagatgt gttgatggat
    12181 cttgatatca tggatggtat gatgttcaat ggacacgttt cggacgagat tatgggaaat
    12241 tcgcaaaaaa tgcgttctta cttacgtggt gcttttatgg cctcaggttc agtcaataat
    12301 ccggaaacga gccgttatca tttggaaata ttttctattt atgaacagca taaccaagac
    12361 atttgcgata tgttaaatta ttacggtcta catgctcgaa cgcttgaacg gcgcaatggc
    12421 tatatttctt atttaaaagg agcagaaaaa attgctgatt tcttaacatt gattggtgca
    12481 accaactcaa tgctccgatt tgaagatgtt cgaatcgtac gggatatgag aaattcggtc
    12541 aatcgtctag tcaattgtga gacagcaaat ctgaacaaga cgattgatgc ggcttctaaa
    12601 caaatcgaaa acatcgaatt gatcgaagca aaggtcggtc tacatgccct tcctgaaaaa
    12661 cttcaggaaa tcgcagaact tcggcttcaa catccagagg tcagcttgaa agaattaggg
    12721 gaaatgattc cctcaggtgc gatatcaaaa tcgggaatta atcaccgaat tcgcaaaata
    12781 aatgaatttg cggaaaaact gcgtgaagaa gcagtttaat ccaaagaaaa aactgataca
    12841 tgaagcagga gatgcgaatg tatcagtttt ttttaaaagt tatacttgct tttcttccgt
    12901 caaactataa ttccatcttt actggagtaa cataatttgg aaaggcttct attgttttta
    12961 atacaatcgt ataaaaagcg gggagacaac tgaatgaaga ttttagtttt gtacataaat
    13021 ttttgcttaa tatagaagtc ctgatataga taagatcaag tatttatcta taactggcct
    13081 gattaattaa aaaactggaa ggaactgctc gatcatcctt gagaactgct cttcttactt
    13141 ctggtcaaga ttctcgtggt tggtggataa ggatggttga taagttcatt ggattagcta
    13201 gtcgaaaaca accaggtgta attaatgtag aagttttaac aaggggatgg agtgatttat
    13261 tcagtgataa tcataagttc ataaatagta attgagatgt atggttggaa tacgttatac
    13321 ctgaacttga actaccaaga tttggtttag aatttgagaa tcgatgttta taaagggagg
    13381 gcaaaaatgc gtttttttac aacttttact atcattatga ttgcagtact ttttattttt
    13441 ctagatatag caaagcgaaa tacagcattt ttattatatc gggttttact tcgagcaggt
    13501 ctcataacat ttatatcgat cgttggattt tttctattca cagttattgt atttatttgg
    13561 agaactccag caccaccatt gccagagatt acatatggag aatttccttt tagacttgag
    13621 tatgaattga atgaagaatt acatgtgata gaagacacgt taatagttga atttgatgga
    13681 tttggaatga acgaaggcat agggagatat cgtagatgga caagtcgttt agccagcgga
    13741 gaagatttag tgttgctgtt agaagttagt gataataagc aaatatttta ttttccagga
    13801 cctgctaatt attacatggg agataggttg aatggttata atcatacttt tcctagtgct
    13861 tctttcattg aaagggaaag aggcattact agaagggata ttttacatga caaagaatta
    13921 ttagaacaat ttggtccgct tgatcaaaat acaataaatg aagaagaatt gttaaatcaa
    13981 tataatataa ggctagtgaa ttgggaaata tctgaaccga ttgttaacaa ttttggtgat
    14041 tagaaaacaa ttacgataaa tagaaacaag ccaaatcatc agcaagtaaa agctgatgat
    14101 ttggcctttc ttatacgagt agtaggaatt catagcgaat attattacga tcagtagata
    14161 ttttgatatt aaaggagtat tacgaactag tatgtgagaa tgtagaggtg atttgcgaat
    14221 aatgagaaat ttatcaaata gaaacaaaat attaatcatt attgttgtta tagctgtgtt
    14281 tcatctggga acgaatgcgg ttttaagtag aataatattg ggtcctaagc caccaagacc
    14341 tgcgattacc cgaggggagt ttgattttag attagaatat gaagtagatg gggaaaggat
    14401 tgtgatagaa gatacaattg ttgcattatt tgatggattt tctgcagatg caggtagcat
    14461 ggcatggtat cgcacgtggc gtttgcattt ggcgagtgac aagagggcac aacaattatt
    14521 gttacatgag ttagatgatg gtcgaaaaat atattatcat tttggaggag ctggatttta
    14581 tatggggaag ttagtagcag tgtaactgta acaaggaaag atttgccagg tcttggcatt
    14641 cgaaactcca tattttatat cgagcgtggg agagatacat ctattgggaa caggaaaggg
    14701 ttaactcttg aagagttata taatgacttt ggaatccgtc ttgttagttg ggaacacaat
    14761 ccaccaatag aaaatcgctt tgaataatcg aaatctatta atccatttca tcagccagtg
    14821 aatgttgtaa tggaacaatt tggtttagtg ttcaaaacca ttgaaatact aggctgtgat
    14881 cagcaaaaat gtatctgaat aaaaaagaga gcaactatta aatgagagga gttaggagtt
    14941 gttagcttta tacgaatcaa agtggcaaat acagatgcgt atgcatttgt gtttgccatc
    15001 ttgcgtttgg tagacgagat aggtataagc taaagaaaca acttgtttaa aataaacgac
    15061 ctagctcaac aaacgaccat ctaatcaact aacagcatac ataaaagcaa ctatttttga
    15121 agtagaattg aaatcgggat aacttaaaaa gtgattggga tactagtgtg aatcaccgta
    15181 cataaatatt actcgttaag ggaatttgag aaattttgta tgaaaattta agaattagtt
    15241 agtatgcgca agtcaaaaat atgaataaat tatttggtgg agataatttg ctcagcttgt
    15301 cataagaaat gagataatct ggcagatgca agcttttgga caccatttat tcggaataag
    15361 gaggctggga cagaagcgtt taactccaag aaataagaag gagttgcttc tgcttccgcc
    15421 gtttataggt gtttagagcg tgagacaaaa gtgttcttta cttttgtcct acgctcctta
    15481 tttttttatt ttgtttggat caattctttt aattcctcta ttgaaataga ttcatcaaag
    15541 atttctttac ccaagatgac agtcggaaca aatcgaatat gtgcttgctc agcttcgttg
    15601 atgacagctt ggctcagctg ttcgtctttt tgttctgtca gctttagttt atctactgca
    15661 aaatccgcta cttcttggag agaaagatgt ttccactcgt cttgagtatc gaatatttgt
    15721 ttgatctctt tgatcgcttt ttgaccatcg ctgattgtga gataccggtg catcacgttt
    15781 cctcgctgca agctttcttt ttctttatcg aacaatttga tcactcgctg caattgcccg
    15841 ctttggactg cttcttctaa tagctcataa gattcttcaa accattgtcg gcaatacgga
    15901 caagataggt tgatgaattc gaccattttt ttaggagcat tcgatgaacc ataaaggatt
    15961 cctttttgtg tatttgtttt cgttgcatca atcactgata tatccatgtt aaaacacctc
    16021 tttcgcttat agtttataca tgtctgacaa aaaaaggcaa cgatttagac tgtttgtcta
    16081 aaaattagat gaccaattca aatagacata tgtgaaatat ttttataaat aaaagaaaaa
    16141 atggaatgtt ttacgttata atggaacaga taataagaga gaatatgaac aaggatgatg
    16201 aatcatgcga aaaatgaaaa caatggatgg caatacggca gccgcatata tttcttatgc
    16261 atttaccgag attgctgcaa tatatccaat aacacccagt tcgaccatgg ctgaattagt
    16321 agatcaatgg tcagcagcag gaaaagaaaa tgtattcggt caaccagtaa aagtagtaga
    16381 aatgcaatcc gaagcaggag cagctggtgt ggtccatgga tcattgaaaa ctggtgcact
    16441 gacaactaca tataccgctt ctcaagggct gcttttgatg ataccgaata tgtacaagat
    16501 tgctggggaa ctactgccaa gtgttttcca tgttgctagt cgggcagtga cgacaaatgc
    16561 gttgaatatt ttcggagatc acggagacgt gatggctgca agacaaacag gatttgctat
    16621 gttagctgaa agcagtgtac aagaagtgat ggatttagca cctgttgccc atcttgcttc
    16681 tattgaagga gaagtacctt ttttgaattt ctttgatgga ttccgtacga gtcacgagat
    16741 tcaaaaaatc gaagttttgg aatatgaaga attagctcct ctgatcaatc aggagaaatt
    16801 agcttcattt cgtaaacgag ggatgaatcc caatcatccg actgttagtg gtacgaacca
    16861 gaatccagac atccattttc aacaacggga aacgatcaac ccctattatg ataaattacc
    16921 tgaaatcgtt caaaaatata tgaaagagat caaccgatta cgaggaactt cttatgactt
    16981 ggtgacttat tacggagcag acgatgcgga agaagtcatt gtttctatgg gatcagttgc
    17041 acagacgatt gaacaaacga ttgattactt gaatgcaaac ggacggaaaa caggtttttt
    17101 gaatatccat ctttatcgtc cttttccagt ggaaacattc gtgcaaaagt tgcctcatac
    17161 agtaaaaaca attgctgttc ttgatcgaag taaagaacca ggggcaggtg gagaacctct
    17221 cttattagac gttcaaagtg cgttatatga ttctgaactt cgcccggctg ttattggtgg
    17281 tcgctacgga ttggggtcta aagatgtgac tcccgaccaa atctcagcag tttttgatga
    17341 attgaaaaaa gacccaagca tacgcaaaaa acggtttacc atagggatcg tggatgatgt
    17401 gacttatcaa tctttggaac caaaggaaag tctggatttg acagaaccgc aaacattcca
    17461 ggccaagttt tggggatttg gatctgacgg aacagttgga gcaaataaat cagctatcaa
    17521 gattattgga gatcacaccg ataaatatgc gcaaggctat ttttattatg attcgaaaaa
    17581 atctggcggt ctgactgtct ctcatcttcg ttttggagat acgccgatac gttcagcgta
    17641 tcttgtcgag catgcggacc ttgttgcctg tcacacacca gcttacctgc atagctatga
    17701 cttggtcaaa ggattgaagc caggtggcat ttttttattg aatactcttt ggagcgacga
    17761 acaattagaa acgcatctgc ccttaaaatt aaaacgttac ttggctgaaa ataacattag
    17821 attctatacg atcaatgcca tgagattggc ccaagaagtc ggtttgggac gccggatcaa
    17881 tacggcaatg gaaacggctt tttttaaatt agctgacatc attccttttg atgaggtgct
    17941 tccgctgcta aaagaagaag ccctgaaaag ctatggacat aaatcaatgg ctatcgtaga
    18001 aaaaaacata caagcaatcg atcggacgac cgaactgctt catcaagtcg cgatcccaga
    18061 ccattggaga acgttggaga tgcctgaaaa aatcaataaa aggaacactg gttatataca
    18121 agaaatactt gaaccagtga atgcacagga aggaaatcaa ctctctgttg gtacacttgt
    18181 agcaaatgga atgacaactg gcgaaatgcc tttaggaacg gcttctatgg aaaagcgggg
    18241 cgtcgcttta gaagtacctg agtggattag tgatcgctgt acgatgtgta atgaatgtgc
    18301 ctttgtttgt cctcatgctg ccatccgtcc atttttagca gatgaagagg aaatggaaga
    18361 agcgccagaa ggatttatcg tgagagaaat gcgaggcgca gacggcgtaa aataccgtat
    18421 ccaagtttca gtggaagact gtacaggttg tggtctgtgt gtagaggctt gtcccgcaaa
    18481 agaaaaagct ttagtgatga agccttatga agaacagaaa gaacaagctg tgaattgggc
    18541 ttttgcaatg acattaagac aaaaagcaaa tccagtcaaa ggaaagaata ctgtcttgtc
    18601 tacacaattc aatcaaccgc ttcttgaatt ctcgggcgct tgctcaggct gtggcgaaac
    18661 accttatgta aaattattga cacaaatgtt cggtgatcga atgatgatcg cgaatgcgac
    18721 aggctgttct tctatttggg gcggtgcttc tcctgcttct ccttatacga ccaatgaatg
    18781 tggtcaaggg cctgcatggt caaattcttt attggaagat aatgctgaat ttggttatgg
    18841 tatgcttcta gcggcacaga ctcgtcgcga ggctttggca agttcaatca aagaagtatt
    18901 gccaaaagta tcagaagaac tacgagaatt gatggaagac tggttggtac acatgtttga
    18961 gagcgaagga acgcagcaaa gagcagcaaa actgcaagct gcgatcgaaa gcgaacgatc
    19021 aattgtccct gaactggagg atatatacaa gagaaaggat ctattcgtaa aaaacagcca
    19081 atggatgatt ggtggagacg gatgggctta cgacattggt tttggcggga tcgatcatgt
    19141 acttgccagc ggagcagacg tgaatattct tgttttggat aacgaagtct attccaatac
    19201 tggcggtcaa acctcaaaag caacaccagc ttcggcaatc gctaaatttt cggctagtgg
    19261 aaagtatgct tcaaagaaag atctaggaat gatggcgatg acatatggaa atgtttatgt
    19321 ggcgcaaatt gcttcaggtg ccaatcaaat gcagacgata aaggcttttg aagaagcaga
    19381 aaagtatcct ggaccttctt taatcattgc ctatacgcct tgtatcacac atggtttgct
    19441 tggtggaatg aaagaatcgc ttaaagaagc aaaagaagct gtttcttctg gctattggtc
    19501 attatatcga tataaccctg caatggtcga aaaaaacaag aacccaatga ctttggacta
    19561 caaaaaacct gatttctctg ctatgcctga tttcatgagg aaacaagttc gcttttcctc
    19621 gctagagaac gcaaatccaa atcttgcaaa agctttattt gaaaagacta tccaagacgc
    19681 taaaacacgt ttttacaact atgctgcact gacgggagaa ttagaaaaaa tcaagaaacg
    19741 acttgaacca gaggaatcta tcgatgtacc cgttaagact agaaaagaaa aaacgacgga
    19801 ccttgaagca gaagaaagaa gagctgccag agcagaacga cgtaaaaaaa tggaacaagc
    19861 tacaaaagaa aaaaaggaaa aataacagaa aaaatataat gaataattct ctgaagttta
    19921 aaaatattta ttgcagagaa aaataaaacg catacttgca attttcctga aaattatgcg
    19981 tttttctcaa gaaaaatacg agcagatcag cctctaaaat taaaacatat gatatactag
    20041 agaggaaatc tcaaaagaga agagaggctt tttatgtcat ttcaaatcgg ggaacttttt
    20101 aatttggact attggcagca aatgttttcc ggtgacttgt tctctctgaa ttttttggtg
    20161 aacatacttg atattctggt tgtctggtat cttgtataca aactgatcca actgctgcga
    20221 ggcacaaaag cgattcagct tctgaaaggt gtagctattt ttattgttat ccgaatcgta
    20281 gcggaaatca ttggtctgca caccttgtct tggctgatga accaagtcat tacgtacggg
    20341 gtgatagctg ctatcgtgat ttttcagccg gaaatccgga gaggactgga acatcttgga
    20401 cgcagttcgc ttttcaaaca atcaaaaagc gaacagcagg aagatgagaa gatgatcttg
    20461 tcttttgaca aagccatcca atatatgtcc aagcgaaaaa tcggtgcgct aattactatc
    20521 gaacggcata ctggattaga tgagtatatt gagacaggaa ttgcattaga tgcagatatc
    20581 actggagagc tgctgatcaa tatctttatt cctaatacac cgctgcatga cggtgcagtt
    20641 attgtaaaag aaggaaagat cgcagtagca agcgcctatc ttcctctatc agaaagtatg
    20701 ttgattccaa aagaattcgg gactcgacac cgggcagctg tcggtataag cgaagtcagc
    20761 gacgcgatta ccatcgttgt ctctgaagaa actggggatg tcagcattac attagataat
    20821 gaactgatgg ctggcttatc ccagcaagaa tatttggcaa ttttgcgtag agaactgatt
    20881 gctgaagaga aaacaaaaaa caagaaaaac gtgattcaat ctttcctaga cggtatgact
    20941 aagggagggg atcgcaaatg atctcaaaag agaaacggac aagcattctt tacagtttta
    21001 ttgcattgct gttcagtatt gcgctgtact tcaatgcaaa tggccagtcc gttccaaata
    21061 cgctttctgg aaatgaagcg tataatcaaa cagttactgg ggtgccaatc aagatttctt
    21121 atgattcaaa gcgctactat atccatggat atgaaaatgc agtgactgtc aaactttcca
    21181 gtgcaaaccg agttcagttg aatgcagagg caaacgaaga tacacggatg ttccgcgtca
    21241 cagcagatat cactaagctg ggcgaaggaa cacatgaggt tccgctaaaa attcaaaatt
    21301 tgagcagtgc tgtcacagca aagatcgaac catcgactat tacagtaacg gttgagaaaa
    21361 aggtaataaa agatttcgat gtggaaacgt tgattccttc aacgttcaca ccatctggat
    21421 atgaacttga tgatacttcc gttagtccta aaaaagtctc tatcactaca ggtgacaaaa
    21481 cattggcaga aatcaaacga gtagtagctg atgtggacgc ttcgatggtc acagatgatg
    21541 ggattaatag tgaggtccca atacaggcgc tgaatgcagc aggggaagta ttaagtatcg
    21601 tctctgatcc agtgcaagta acagtgagag ctgattcgat caagccaagc aagtcggtac
    21661 gactttatgg gatacagcaa ggaacaccag ctgccggggt agaaagctat gatttcagtt
    21721 tttcaaaact ggaagcagaa gtaagcggct ctagcgattt gcttgcatcg atcggtgaca
    21781 gtattgctgt accgatcaat gtctcaggta tcaatcatcg aacaactcgt aaaattgaaa
    21841 taccagtaga agaggggtta agtatcaagc ccgaatcagt tagtgtggag attacacccg
    21901 tgttgcaaac gtcaagcagt agctctgctt cgacgagcag tcggacacaa gaaaatacaa
    21961 gtacgagtag aagtatttca tctgctgatt caggaacgga accatcgaca acaaccattt
    22021 cttccagttc ggaatctggg acaagcagct cgaccacaag tacagaagaa acatcagaaa
    22081 caagcgaatc aacacaaatg agcagaacaa attcctcaga aaattaagtt aaaggagtaa
    22141 ttttacaact atgggtaaat attttggaac agacggcgta cgcggtgaag ccaacaagga
    22201 acttacgcca gaacttgcat ttaagctggg acggtgcgga ggttatgtcc taagccagca
    22261 tgaagaagaa ggaaaacgcc cacgcgtgtt agtagggcgg gatacaagaa tctcaggtca
    22321 aatgttagaa gaagcgctga tttcaggact tttatcggtg ggtatcgaag tgttccagct
    22381 aggtgtgatc tctacaccag gtgttgctta tctgacacgt ttgcaaaaag caagcgcagg
    22441 ggtcatgatt tctgcttctc ataatccagc acaagataat ggaatcaaat ttttcggtgc
    22501 agatggtttt aaactagtag atgaccaaga gctagaaatt gaagcattat tagatgctga
    22561 agtggatgaa ttgcctcgtc catcagctga aggattggga actgtagaag aatttccaga
    22621 aggcttattg aaatattctc aattccttgt acaaacgatc aacggtgacc tttccggttt
    22681 gaccatttgt gtcgatgcag caaacggagc aacttcaaca agtgttaatc gtttgtttgc
    22741 cgatcttgaa acagattttt ataccatggg cacaaatcca gacgggttaa atatcaatga
    22801 tggcgtaggc tctacccatc cagaacgttt agctgagatg gtcgttgaaa aaggagcaga
    22861 tgctggacta gctttcgatg gcgatggcga tcgtattatt gcggtggatg aacttggaaa
    22921 cattgtcgac ggtgataaaa tcatgtttat ctgtgctaga tacttggcag aaaaaaatcg
    22981 attgaaaaaa gatacgatcg ttacgactgt catgagtaac ttaggattcc acaaagcggt
    23041 ggaaggaatc ggtttgaaag atgtcgttac acaagttggt gatcgttacg ttgtagaaga
    23101 aatgcgcaaa aacgattata actttggcgg cgagcagtct gggcacatga ttttcttaga
    23161 ttttaacaca actggagatg ggatgctttc aggaattcag ttactgaata tcatgaaaca
    23221 gacaggtaag aaattatcag agcttgcagc agaggtaaca atctatccgc aaaaattagt
    23281 gaatatccga gtaagtgata aaaacggtgc gatggatgtt ccggcaatca aaaaagtgat
    23341 tgaagaggca gaagctgaaa tgggatcaga aggaagaatc ctagttcgtc cttccggtac
    23401 agaaccatta cttcgagtga tggcagaagc gcctactgat gagaaagtta attactatgt
    23461 cgataaaatc gcggcagtag tcaaagaaga aatcggactg gattaagaga tattttcata
    23521 aaaaataaaa gaacaaggac cagtgtcaga tgtctgacac tggtccttgt tcttttatta
    23581 ttctactgtc actgatttcg ctaaattacg tggtttgtca acgtcgaatc cccgtaatag
    23641 cgttgcgtaa tatgcaagta attgagtcgg tacgactgtg gttagagtag ttagataagg
    23701 gtggacagtt ggtagcacca gctggtcact ttccttttct aatccctctg agacaataac
    23761 tagtgtttta gcgccacggc tttcgacttc tttcaggttt cctcgagtat gcgcaccagt
    23821 cacttcttca ctgatgattc caatcactgg tgttccttct tcgatcaaag caatcgttcc
    23881 atgtttcaat tcgccagcgg caaaaccttc tgcctggata taactgattt ctttcaattt
    23941 caaagcagct tcaagagaaa cgttgtagtc agcgccacgt ccaatgtaaa aggcatttct
    24001 tgtatcgctg agatatactt ctgccaactc ttctaaataa tctttttggg cgatgaccga
    24061 ttccatcgca ttagcaacta aactcaattc atgagtgata tcgaatacaa gagaagtttc
    24121 aaattctttt ttatcaccga tagctttggc taataaagtt agtacagcta tttgtgcagt
    24181 ataagctttc gttgatgcta ctgcgatttc gggtccagca tgcaatagca gtgtaaagga
    24241 tgcttcccgc gaaagagtag aaccagcaac atttgtaatc gtaagtgaag ggtagccgag
    24301 acggttgatt tttaccagaa cttggcggct atctgctgtt tcgccacttt gactcaagaa
    24361 aataaagaat ggtttttttg aaagaagagg tgtgttatag ccaaattcac tggctaaatg
    24421 aacctcaaca gggatctgtg tcaatttttc cagagtctgt ttacctgcta accccgcatg
    24481 gtaactagtt ccgcaagcaa cgatatagat tcgatcgcta tcagacaatg tatcgaccag
    24541 cttttgatca agagagacac gattgtcttc tgttacgtat ttttgagcga tacgacgcat
    24601 gataataggc tgttcgtcga tttctttcag catataaaaa ggataggtac ctttctcgat
    24661 atctgaagaa tctacttgtg cttcgaatga agaacgttct acgatgtcac ctgcaaaatt
    24721 ttgaataata actttttctg atgtgacggt aaccatctct tcatcttcaa tctcaacgaa
    24781 ttccttcgta tgagcaagca tagccattgc atcgctggca atcacattga atccatcgcc
    24841 gagtccgatt aatagtggac ttttattttt agcgacgtaa ataacatcag gtgctgtccg
    24901 atcaatcaaa gcgaaagcgt aagaaccttt gatgacccgc aaagctttta aaaacgcctc
    24961 ttgcgttgta gtatccttcg cgaaagtttc aattagatga gcgataatct cagtatcggt
    25021 ctcaccaata aagtggtcat ttgctaaata agcaatttta agttcttcaa agttttcgat
    25081 tacgccattg tgaacgagca cgaagcgtcc actttgggaa gtatgtggat gggcgttttc
    25141 ttcagaaggt tgcccgtgag ttgcccagcg tgtatgacca atacctatat ttcctgtagt
    25201 atcaatattt attttgtttt tcaaattttg aattctgcct tgtgctttga ccagataatc
    25261 agttgtgcca tctgagacaa agataccagc tgaatcataa ccacggtatt ctaatttttc
    25321 tagaccgtca atcaatcctg gtgttacatt ttttaatcca atcattccaa caattccgca
    25381 cataaaaaac atcctacttt ctataatgaa ttcgtatgaa tgcttccagc tgcaggaaaa
    25441 tccttttgtt ttatctttgc agatattttt tgtggatttt ctgtagagtg cagtccctag
    25501 ggccatccgc cgaatctttc gataaaccct ctcctcgtca tctgtttaca cagacctggc
    25561 gctatcctaa aaggtataac tatcctcctc ttttgttttt tatctggaaa catttaaaat
    25621 cataaccttt tgcgaatgtt cttgtcaaca aaaaaagaat aaaaaataaa ttggtatata
    25681 tcaaatgctt tgtcaaatgc tctttttatg tcaaaaaata atgggttttt atctatcatc
    25741 tttttcagga gttcggtaca atagaaagta atcggataga ggaggagtaa aaatggattc
    25801 aaaaattgag atcatgacac tgggaatgtt aaaaaaacaa ttgtccaaat tcgaagcatc
    25861 agctggtgta tcagatgata cgaaaatatt tttagatact ggctgggata gcatccaaga
    25921 aatcgcacct gatgccttgg aagttgctga agctcgtgag tttacggtag aagatgaatt
    25981 gacaaaagag tcgttttccg ggtatgcgcg ggaagaaaaa gcagagcggt tcgacacttc
    26041 tgagcagtca gaaactgtga tcgtcataaa aaacttgtat tagaaaggag aaagaatgaa
    26101 taagagacgt tgggcagcag ttctgattgc tgcaggatta tttgtggttt cgttagtttc
    26161 agctagtttg actaagcctc aggaaaacga tcaaattgag ggattgaaca gttggttata
    26221 tggggatgaa gaactagctt caaatgtgtt ggaagaggga gacagtacgc aaaaaatcgt
    26281 aaaattatca gtagatggaa cgattgctgc gggagggtct tcaggtttat ttgcttctga
    26341 aggttacaat cataaaaatt ttatggaaca actgaaggcg attgaagaag atccaacagt
    26401 caaaggtatc tttttagaag taaattctcc tggtggcggt gtctacgaaa gtgcggaaat
    26461 cgctaagaaa ttggatacga ttcgtaaaga acatgatatc ccaatgtatg tcagcatgaa
    26521 aaacatggca gctagcggtg gctactatat ttctgctcaa gcagataaaa tctttgcaac
    26581 agaagaaaca gtgaccggct caattggtgt gatcatgtca ggattgaatt attcaggact
    26641 tctagaaaaa ctgggtgtgg aagatacaac agttaaaagc ggagcgttga aagatatggg
    26701 ttcaggaacg cgaccagaga caaaagaaga agaagcggtg cttcaagcat atatcgacag
    26761 cgcatatagt cgttttgtag caatcgtcag cgagggacgg aataaatctg aagaagaagt
    26821 caaaaaaatt gctgatggaa ggatttatga tggtgtccaa gcaaaagaag taggtttagt
    26881 cgatgaattg ggatttcctg aagatgcttt aaaagctatg cgaaaggaac aacagcttga
    26941 agatgcggaa ctagtagaat attcttccac gccatctggg tttgctagta catggttagg
    27001 aagtaaacta gcagaattcc agggattgaa gccttctgag actagtcaga tcttatccgt
    27061 tcttgagaat ctagggacag cggaatcacc aaaagcaatg tattattacg gaggtgagta
    27121 agcatgtcag agctttcaaa tgaaaataag gaacaagaaa aagataagca acagcagcct
    27181 gctgtaggac ctgactccaa cttttcccaa aaggtactga gctcttttga aagagagcct
    27241 ttgacgcccg aagaaatcaa aaagaaacaa cgtaaatgga aaaagtatac ggacacaaga
    27301 aatgaaccgc caaaagcaaa tgattttcct aagtatttct atgcaggttt ttttatccga
    27361 atgtttgctt tcttggtcga tcttttgtgt atcggagcta ttacgcgttt aacacttggt
    27421 attgccaaaa atctaggttg gcttaatttt tcagattctt atctgagtat ctacggattc
    27481 acagccttac ttatttatct agcgtatttt attttattga cgaaattcaa tcatggtcaa
    27541 acgattggga aaatgatctt tggtattcga gtcatcagct ttgatgaaga agaacttagc
    27601 tggtcaaccg ttttagttag agaaggtgct tgccggttca ttctaaaatt tcctttgctg
    27661 atgttagggt atcttccagc aatctttaat cagaaaaagc aacatctagg cgattttttc
    27721 actaatacaa gtgttgttac gatcaatctg atcaaagcat tcaaccagga agtcaacagc
    27781 tgatgagcaa taaaaagcag taacagttga aagcaccttc taaaagtcag atctaaaatc
    27841 tagcttttag agggtgcttc agttattttt aattgtcatg ctttttttgt tttgctacta
    27901 ttgaacatga gctgaacatg agcaagagga ggacactgcg tgaagccgat tgattttcca
    27961 gaaaaatatg agaatttaat gcgagtagca caacaagcac tagcaaacca gcaatatcag
    28021 caggcaaaag aattatttca acgagcttac gagttgaaag aaagctttga ggctaatagc
    28081 ctgcttgttt tttgtcttta cgagttggac gagaaaaaag aagcattgaa gcaagcactc
    28141 ttgcatgaaa aacagtatct tcaaaatgag gagtttgctg aattttattt tgacttgtta
    28201 atcagtacac aagatttttt atatgctaga aaattgatag caagtacaga tttttatgaa
    28261 gctttcgagc agcgggtcat tgaaaaaatc caatttgcag aagaattatc cggacaaatg
    28321 gagaggcaga agatcaaggc actccacaat aaatcggagg aacttccttc gttggaacct
    28381 gcacgtcagc ttgcgtctat tgatgagatc gaacagcttc cttatcatga gtttatccaa
    28441 acagcgagta aacttattgt atcgccagag gtccatattt tagcaagggc aaaattactg
    28501 gagacactta ggcaattgaa cgaatgccat cctgttttct atttgacgat tgaagaaaag
    28561 ttagtcaaag tcataccaaa agatctgcca aagccgcagc agcaggcaag ttaccagcaa
    28621 ttatgtgtat tttcagatcg ttacggtaat gaagatgcgc tgcttagttc cgtgttaaat
    28681 gaagaattca cgttgcaatc tgcaattgtt tatcctgtct atgatacata catcgaagac
    28741 ccgaagcgct ggtttcaact gactgtagag gcgtgtacag gaaaaacgat gtatgaaggt
    28801 acagaagatg aaaaacaaga ttttttaaaa aaaagagaaa aaattttgca gcaaatgatc
    28861 ttcttccatt agattttatc agtgaaaaag ataaaaatgt tatgaaaaac aaggattcta
    28921 tccattcaat atgtttacaa tttgaaaggg ttagtgtact atttaaaggt atgcatgaaa
    28981 cgtttttgat gtataaaatg aatttctaaa ctcggaggga attaaatgac tgcaacattt
    29041 gaaaaaaaag gcacgaatga tggtgtttta aaatttacga ttgcgcaaga cgaaatccaa
    29101 aaaggattaa caactgcgtt taataaagtg aaaaaaagct tgaacgtacc tggattccgt
    29161 aaagggaaag tttctcgtca agtattcaac cgtatgtacg gtgaagaagc tttatatgaa
    29221 gatgcattga atgctgtatt accagaagct tatgaagctg ctgtcaaaga agctggcatc
    29281 gatcctgttt ctcaaccaaa aatcgatgtt gaaagcatgg aaaaaggtca agactgggta
    29341 atcactgctg aagtaacagt gaaaccagaa gtaaaattag gacaatataa agatctagtt
    29401 gttgaaaaac aagaccgtga agtaacagat gctgacgtgg atgcacgtat caaacgtgaa
    29461 caagaagcac aagctgaatt agttatcaaa gaagatgctg cagctgaaaa tggcgataca
    29521 gtagtaatcg acttcgaagg ctttgtagat ggcgaagcgt ttgatggcgg aaaaggcgaa
    29581 aattactcac ttgaattagg ttcaggctca ttcattccag gttttgaaga tcaattagtt
    29641 ggtcataaat caggtgaaag tctagatgta aatgtaactt tcccagaaga ttatcaagca
    29701 gaagatcttg ctggaaaaga agcagtcttc aaagtgacaa tccatgaagt aaaagcaaaa
    29761 gaagtgcctg aattagatga cgaattcgca aaagatgttg atgacgaagt atcaacatta
    29821 gacgaattga aagaaaaata tcgcaaagaa ttagtagaaa caaaagaaaa agcagcggac
    29881 gatgcaaaag atgaagcagc gatccgtatg gctgttgaaa acgcagaaat cgttgaattg
    29941 ccacacgtga tggttcatga tgaagtacat cgttcaatgg acgaattctt gaacaacatg
    30001 caacgtcaag gtatctctcc tgaaatgtac tatcaattaa ctggttctac agaagaagac
    30061 ttgcataaac aattcgaagg tgaagcagaa atgcgcacaa gaacaaatct tgtgatcgaa
    30121 gcaattgcag ctgctgaaaa tcttcaagca acagaagaag aaatcaatgc agaaatccaa
    30181 gaattatcag aaacatacaa catgccaatc gaacaaatca aacgcgtgtt gacagaagat
    30241 atgctggaac atgatgtgac aatgaaaaaa gcaatcgaat tgatcacagg tacagcgaca
    30301 gaagcgaaag aatagaaatc aagtaataaa tcatttctga agaagctact tctagaaaat
    30361 tgtttattcg aaataaggga ctgtgacacg attttgtcac agttccttat tttttgttaa
    30421 gagtaaaaaa tgcaaaggta taggtcagtt gatcctttgt taaacacttt ttctttttac
    30481 taaactatgt tatgatagta aaagactgta aagtctttga tagtttcttg catacttttt
    30541 gcaagttaag gggtgaagac catgtatgac aatccaagta gcaatgagac cgtacgctgt
    30601 tctttttgcg ggaaaaccca agaagaagtg aaaaagatcg tagctggtcc aggggtctac
    30661 atctgtaatg aatgtatcga cctatgtaaa gaaatcattg atgaagaatt ttatgaagaa
    30721 gctgtacgcg aattcactga agtccctaaa ccactagaaa ttcttgaagt attgaataac
    30781 tacgtgattg gacaagatcg cgcaaaacgc tcgttagctg ttgccgtata caatcactat
    30841 aaacgtgtca atcagcaagc acaagaaaac acagatgatg ttgaactgca aaaaagcaat
    30901 atctgcttga tcggacctac agggtctggt aaaacatttt tagcacaaac attagcaaaa
    30961 acattgaatg taccttttgc aatcgcagat gcgacaagtt tgacagaagc tggttatgtg
    31021 ggggaagatg tagaaaacat tttgcttaaa ttgcttcaat ctgctgatta caatgtggaa
    31081 cgtgcagaaa aagggatcat ctatattgat gagatcgata aaattgcgcg taaaagcgaa
    31141 aatgtatcta ttactcgtga cgtttccggc gaaggtgtcc aacaagcctt gctgaaaatt
    31201 ctagaaggaa ctgttgcaag cgtgccacct cagggaggac gtaaacatcc acatcaagaa
    31261 tttattcaga ttgatacgac gaacatattg ttcatcgtag gtggtgcatt tgatggcatc
    31321 gaaacgattg tgaagaatcg tatgggcgaa aagacgattg gatttggtac acaaaaccaa
    31381 aaactttctg aagacgaaag tgtcatgcag catatcattc ctgaagattt actaaaattc
    31441 ggattgatcc ctgagtttat cggacgatta cctgttatgg cagctttaga gaaattgaca
    31501 acagatgatc tggtacggat cttgactgag ccgaaaaatg cgctagtcaa acaatatcaa
    31561 aaacttcttt ctctagataa taccgaactt gaattcgaac ctgaagcgtt gagagccatt
    31621 gcgaaaaaag caatcgaacg caataccggt gctcgaggac ttcgttccat cattgaagaa
    31681 atcatgatgg atgtgatgtt tgatatccct tcaaatgaaa acatcgaaaa agtgattatc
    31741 acaaaagaag cagctgaatt gtctggtgag ccaacagtaa tctataaaga ttcagagaaa
    31801 aaagcgggtt gatgaaaaga ggctgccgta aaagtcattt tggcttttgc gacagccgct
    31861 tgctttattc atcaccttgt taaggagaga gaccatgaaa gtccatcaag ctgaaattat
    31921 cataagtgca gtggcaccaa aacaatatcc agatacacaa ttgccagaaa tagctctggc
    31981 aggacgatca aatgtaggga aatcttcttt tatcaacaca ttgattgatc gaaagaattt
    32041 agcccgtact tccggtaagc caggaaagac ccagacattg aatttttatt tgatcgagaa
    32101 cgcattacat tttgtcgatg ttccgggata tgggtatgca aaagtatcca aaactgagcg
    32161 agcaaaatgg ggaaagatga tcgagacata tttaacgact agagaccagc tacgtgcggt
    32221 cgtttcttta gtagatttgc gtcataagcc ttctcaagaa gatatccaga tgtatcaatt
    32281 tttgaagtat tatgatatcc ctgtaattgt tgttgccact aaggcagata agatccctcg
    32341 tggtaaatgg aacaagcatg agagcgaaat caaaaaagcg ttggattttg atcgcaatga
    32401 cacgtttatt ctcttttctt ccgttacaaa acagggaaaa gaagaagcgt gggcagcaat
    32461 cgaacaagca attgaatcat aaaaagaata aaaaagactt accagtcaaa ctctttcata
    32521 aaagtttggc tggtaagtct ttttttgagc ttaacttact tttgtttctt gtcttttttc
    32581 ttttcatttt gtggctgtgg ttttagaaat cgatcgatct cttcttttgg tgcaactgtt
    32641 ttttccggat cgacagcaaa tgattcgaaa agtttatcta aattatctgt acgttcaaat
    32701 ttcaacccca ttgtaaacac ctctttgttt ctttttactt agtctatcat gtaatcgtcc
    32761 aaattggttt ctaagacata aagttttagt ttttctttat cgttggttat aattaaatta
    32821 taaaaagatt ttaaagaggt gagaaaatga ttggcgaaga taaattattg atgactatcc
    32881 agctcacaga cttaaatgaa gaagaaaaaa aacaggaaat aagcgattgg gctattttga
    32941 cacgtctgtt gatccagcca acatttatcc gctctttcac acaacaagcg gatccgtatg
    33001 atcttcgaaa gcttcaagaa aaaataatgc tagcatcggt aagagacgaa agttggttag
    33061 ttgtaggaaa tgacgagaac gaatgtagtt ttcgcttgga agataatcaa ttattgataa
    33121 aaaatgtatt gtctatctct gttttcaaag aaaagcaatt tttgattcga gacttcattc
    33181 agaaaaaaat gattgctcat ggggtgttcg cctatatgcg agcttatagc gagtttatct
    33241 atcacaatac aaaacagatc tctcaacggc tattgtttga gaaaaaggat gaaatcgaac
    33301 atttacctaa aatgaaacaa caaaacgggg aagtagtagt cgattgcaac caatttccag
    33361 gatacgatct tttttaccaa ggtttatgtt tcacttcgtg ttgggaaatg tattatagtc
    33421 gctattatca tcaaatcatc cctaaaccga tttttctcga catccaacaa gtagaaaaag
    33481 tgaaggaatt agaaaacgaa gtcatatgta tccagcttta tcgagacccc tttaactggc
    33541 aaaagccaaa caatcaaatg tttcaatcct attttaggga ccagcttggg tttgatcatc
    33601 ttgcttggga caatggtgtc ggtttattga aaccgccatt cgttgaatac atttacactg
    33661 accatacgat ccaatctgtc cagtatcaga atgcacaaat gcaacctgtt cctaaaaaga
    33721 atgcttcatt ttttgtaaca aaaagttatg atattcaaca aggagattat aaagaaaggc
    33781 gtgttcgcgg aacactaaat gctcaggcgt attttccttg ggttgacgag aatcgctcgc
    33841 gcatgatgtg ctataaagtt atcgatccga ctgttgcttt ggataatgga atcgaagctt
    33901 attgttatta tattagggaa tatctagaag tagaggtaac agatgaaaaa tatcaagagt
    33961 atcttttatc cttaagaatc tatgttcctt cagaatccct gtcagaatta ccgttaaagg
    34021 aaatcaaaca tagactctct gatgttacct tcaaacgaat aaagaaaaag cggcgaagta
    34081 tccaattcga tgtcaagaaa ggtgagcagc atctgcgtgt gcaattttta gattaccgag
    34141 aattagaaca attgattact ttacaaaaaa tatgaggtga agaaaatgga taaacaactc
    34201 tattatcgaa taatggttcc ggcatcatta ttatccgtct gtctcttttt tgttcctaat
    34261 tttcctattc gatcagtcat cggttttttg gtcatgtttg gcgggatggg actttattat
    34321 tatcttcttc ataaggaaag aaaacctgct tctgtttata tgaaaaaaac aagaagcgaa
    34381 aataaaaaac gataaaacgg cttgat
//
LOCUS       DAYYHS010000021        32666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000021 DAYYHS010000000
VERSION     DAYYHS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.021"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            408..1133
                     /locus_tag="ACHA6A_001026"
     CDS             408..1133
                     /locus_tag="ACHA6A_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM8969247.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            1123..1797
                     /locus_tag="ACHA6A_001027"
     CDS             1123..1797
                     /locus_tag="ACHA6A_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM8969248.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(2085..2369)
                     /locus_tag="ACHA6A_001028"
     CDS             complement(2085..2369)
                     /locus_tag="ACHA6A_001028"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM8969249.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2663..3970)
                     /locus_tag="ACHA6A_001029"
     CDS             complement(2663..3970)
                     /locus_tag="ACHA6A_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8969250.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4459..5565
                     /locus_tag="ACHA6A_001030"
     CDS             4459..5565
                     /locus_tag="ACHA6A_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969251.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            6156..6356
                     /locus_tag="ACHA6A_001031"
     CDS             6156..6356
                     /locus_tag="ACHA6A_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8969252.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            6503..9292
                     /locus_tag="ACHA6A_001032"
     CDS             6503..9292
                     /locus_tag="ACHA6A_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8969253.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            9339..9866
                     /locus_tag="ACHA6A_001033"
     CDS             9339..9866
                     /locus_tag="ACHA6A_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8969254.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            9887..11077
                     /locus_tag="ACHA6A_001034"
     CDS             9887..11077
                     /locus_tag="ACHA6A_001034"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8969255.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            11117..12415
                     /gene="asnS"
                     /locus_tag="ACHA6A_001035"
     CDS             11117..12415
                     /gene="asnS"
                     /locus_tag="ACHA6A_001035"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8969256.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            13349..14023
                     /locus_tag="ACHA6A_001036"
     CDS             13349..14023
                     /locus_tag="ACHA6A_001036"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8969257.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            14020..14361
                     /locus_tag="ACHA6A_001037"
     CDS             14020..14361
                     /locus_tag="ACHA6A_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969258.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            14391..14750
                     /locus_tag="ACHA6A_001038"
     CDS             14391..14750
                     /locus_tag="ACHA6A_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8969259.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            15010..15132
                     /locus_tag="ACHA6A_001039"
     CDS             15010..15132
                     /locus_tag="ACHA6A_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969260.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            15274..17355
                     /locus_tag="ACHA6A_001040"
     CDS             15274..17355
                     /locus_tag="ACHA6A_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8969261.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            17665..19131
                     /locus_tag="ACHA6A_001041"
     CDS             17665..19131
                     /locus_tag="ACHA6A_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8969262.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            19416..19914
                     /locus_tag="ACHA6A_001042"
                     /pseudo
     CDS             19416..19914
                     /locus_tag="ACHA6A_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            19988..20428
                     /locus_tag="ACHA6A_001043"
     CDS             19988..20428
                     /locus_tag="ACHA6A_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8969263.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            20441..20650
                     /gene="copZ"
                     /locus_tag="ACHA6A_001044"
     CDS             20441..20650
                     /gene="copZ"
                     /locus_tag="ACHA6A_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8969264.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            20650..22836
                     /locus_tag="ACHA6A_001045"
     CDS             20650..22836
                     /locus_tag="ACHA6A_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8969265.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            22856..25027
                     /gene="copB"
                     /locus_tag="ACHA6A_001046"
     CDS             22856..25027
                     /gene="copB"
                     /locus_tag="ACHA6A_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8969266.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(25139..27310)
                     /locus_tag="ACHA6A_001047"
                     /pseudo
     CDS             complement(25139..27310)
                     /locus_tag="ACHA6A_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(27300..28052)
                     /locus_tag="ACHA6A_001048"
     CDS             complement(27300..28052)
                     /locus_tag="ACHA6A_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8969267.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(28049..28372)
                     /locus_tag="ACHA6A_001049"
     CDS             complement(28049..28372)
                     /locus_tag="ACHA6A_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969268.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            complement(28386..29261)
                     /locus_tag="ACHA6A_001050"
     CDS             complement(28386..29261)
                     /locus_tag="ACHA6A_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8969269.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            29588..30877
                     /locus_tag="ACHA6A_001051"
     CDS             29588..30877
                     /locus_tag="ACHA6A_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969270.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            30951..31520
                     /locus_tag="ACHA6A_001052"
     CDS             30951..31520
                     /locus_tag="ACHA6A_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8969271.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(31696..31845)
                     /locus_tag="ACHA6A_001053"
     CDS             complement(31696..31845)
                     /locus_tag="ACHA6A_001053"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8969272.1"
                     /translation="MPLEDIKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQI
                     KKHSN"
     gene            31951..32649
                     /locus_tag="ACHA6A_001054"
                     /pseudo
     CDS             31951..32649
                     /locus_tag="ACHA6A_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT        10827 a         5706 c         6791 g         9342 t
ORIGIN      
        1 ttctattata acgaattatg ttgaaaaaag cttttatcga aaacataaat cttcaaatta
       61 acagattttt tagacctcga tcatgatgaa gacttttgta gatacaaata actgtaacat
      121 tgaaaatgaa tgcgaaaacg atattttttt ttattggaaa atatcggtca agcaataaac
      181 cagttgcata ataaaatgtt aacggcaaca aaatctgtgg aaggaatgaa ccggatgatt
      241 tgatggaaaa aacaagatag acaagaagta tacatagtaa aacgtggaac aagtaggcca
      301 gaatgaaaaa tcagttttta tccactcgct tttctataca ttcactgtca caaatataat
      361 cgaaagtaat tctattttga gataaagtat tttataggag atagtatatg aacaaaaaaa
      421 gatttaaacg tattgaaaga aaatttgcat taacttatga ccaagctaag tatttgaaaa
      481 cactatttcc aaaacaggta gttcgggata aatatagtga tcattttatt tcttccttgt
      541 attttgatac aaacgatcat cactttttaa aagaaatcac tacagaaaag tcctatcgag
      601 aaaaatttag aatcagaaaa tatggagatc ctcaggacat atcgcctatc tacttagaga
      661 taaaaagaaa gtttcaagga atgacagata aacgtagaat aaaattagcg catcatgagc
      721 tcgatgatat gttggaaaat atcgaattgg cagaaaccac attcagtaat ttagaagaag
      781 agtatatttt taatgaaata cagtggttgt tcagaaggtt ggcattggag cccaaagtat
      841 atatctatta tttaagagaa gcatttgtaa atagagattc agaagatttg cgaatcactt
      901 ttgatcaaaa tatccattac cgtctaatga acttaacgac ctgggaacac tgtacagaag
      961 acaatctggt agcgccagaa atcgatgtgc tgatggaagt aaaaacgaca ggagaaatac
     1021 ctgaatggtt acaggtagga ttggatagga tgggtaaaaa agagataaaa ttttctaaat
     1081 ataaacaagt ctataaaaga tatatccaag aggacccgct ctatgttcaa tagtgtctta
     1141 aaaaatgagc cttctcaact cacactagat gatttctttt tatgtatggt ggtgtcgatt
     1201 ttgctaggca ttctgattgc ttacacccat atgtatcaaa acaactgtac aaaaaacttc
     1261 atgtttgcct tgattgggtt acctacttta gtacataccg tcattttgtt gatcaacggt
     1321 aacttaggca cggggatcgc tgttctaggc gcatttaact tagtgcgatt tcggtctgct
     1381 caagggaatt caagagatat tttcagtatt ttttgggcaa cgtcgattgg tttagccaca
     1441 gggacgggat atattgtcta tgcactattc gtgtctagta tagttgctgc tatccaagtc
     1501 atcatcttca cgcttccaat caaaaataaa catacagttt ttaatactga aaaaacgatc
     1561 aagctaaaag tttctaaatc cttgtttctt tcgagtatta ttgaagaaaa aattgcagaa
     1621 tatacagaaa actgcaattt aaaaaaagta aaaatcaata aagaaacttg ttccttacac
     1681 tatatagtaa aagtaaaaac gagtatgctt gaaaaagaat tggttcgaac gatcatggat
     1741 catccagaag tcatttcgat cactgtaggt atggataaat cgaataaatt attgtgataa
     1801 ccaagaaagt acttaacaat gaagtaagac cccagttcca aagtttagcg gggacacttc
     1861 aataaagtac tttctttttt gtcttcagca actactggta aaactgagag caagctttcg
     1921 tgagagtaca gcgataaaga tcaacctccc tttttatctc gagcaatttc cttgcaaggc
     1981 tgtttcgttg atcttaaacg agttagcaac agtcaaagat gcgatggcca taaaaaaatt
     2041 gcattaagtt ggctggtatc tgatagctgg gaatctaagg aagactatcc attctgtttc
     2101 ttaaattccg ctagttgata gatatcagcc aattgttcca gatgatcaac atcaaagata
     2161 accgaaagat gtttcctttt attcccattt gcttgaagag tttttatctt ctctttggtt
     2221 ccttcactca atgagagtgt gactaatttc tccctcgttt tttctgggaa atgaaaggat
     2281 actttgaaaa atccgtccca aatagaaagc cagaagatcg gttttacttt ctgagagtgg
     2341 gacaaaagta agaaacactt ttgtctcatt ctttaaaaac gaataaacgg tgggagcaga
     2401 agcaactcct tcggaaataa gccaaaattc acaaaaattt gaaaagcaat tttcgtgaat
     2461 ttcttcttat ttctcggagt taaacacttc tgtcccaacc tcttgtattc accttttgat
     2521 agccaagctt ttccatcatt ataaaaccgc aaatttcaaa aaaaaacgaa caatacaaaa
     2581 agcgtattgc tcgtttttct cttcagtttc tcacacctaa acgacttttg tctcgacctc
     2641 tttttggtga ctattcatga tgttaaaata aattactgaa aaaatctgtg acatggcgcc
     2701 aaccaatgac aaaaatatta gctttttcaa cagtagaagt ggtaatgatg tctgtcgaag
     2761 gctcttggtt tgcttccaaa tagcctaatt tgtcatcagc taattggatc gttgctgagc
     2821 caactgtagt gcctttgttg ataggagcgg tcacttggtc atccgttact ttctttttat
     2881 caattgttgg agtaatcgtt aattttccag tatccattcc attttcgacc cagactttta
     2941 cagggctttt tagcgcaaca ttgacggagg tttcttttcc atctttcact ttgatgtctt
     3001 ttaggcttgg gatgcttgca tttgcttttc caagttcttt ttctgaccag ttgtcataac
     3061 aataatccat caatcgtgat gtttcattga atcgggcact tggattttct tcatggttat
     3121 ctgcatttaa aacgactgtg atgattcgtc ggccatcttt gttgatcgtt ccgacaaagc
     3181 atgcacccgc aaaatcagtt gtgccagttt ttaatccatc gacaccttcg tgatagttta
     3241 caaaaccagg tagcatccag ttccaattga ccatttcaac cggtgactgt gtgttttcgc
     3301 cgaatgtttg atttgtggta ctcgtgattt ttaagacttc aggataatct aagatgaggt
     3361 gacgagcaac aatagcgaca tctttcgcag acagttgatt ttcatctgtt tcaccagtat
     3421 tctcaggtcg attctcacct aggtaagaat tgtttaaacc tgatgcattg acgatcgttg
     3481 catctttgat accccaatct tttagttgtt tcttcattag ctcaagaaat ttactttcac
     3541 tgcctgcaac tttttcagct aaagcaacca tggaagcatt ggctgattgg atgaaagctg
     3601 agtcaacaag ttctttcact gtataatcat tttctttatg caaaggaaca ttcgatagat
     3661 ccggcacgac acttaatttt tctgcataat ccgaaatggc taccttatct tcccaagata
     3721 atttaccctc tttgatttgt tcaagtacga tatatagacc aatgatcttt gtgatggaag
     3781 caattcccat cgttttttcg ccatcctgat cataaaggat ttttccactt tctgcatcta
     3841 cggcaaaggc agctttggca tttattttaa atgcgtcgtc tgcttgaaca gttgcgctag
     3901 gcaagaaaga agtgatacat aagataagac ttgctaatag tacaataaaa cgggtatttt
     3961 ttttgaacat ctttgttctc ctcattttta aattgattct tacaacttag aatattatca
     4021 gagagaacaa aaggtttcaa tggttctgaa aaaaacctta taaagaacaa gtcttattaa
     4081 tgaagaatca tagaaagtta tcttttgggt tcattgacac gaactttgac tggctgaact
     4141 tcgggttcgg ttaggcgtga tgctgcaaaa agggcgcctg ctgagagtgc gaccaaacta
     4201 aaaatcatga ataattgcat aagatcacct cgttcttttt tgttttttta tagtatagca
     4261 agagagtttg aagctactgt taaggaaatg agcatataat tgatgaaagt atgaaaaaaa
     4321 tatgaagtag gacaacacaa ctaaaaatcg atcaggtatt tctctggtag ggattcgttt
     4381 tattgtattg ataaaatggg atgtggtaga ctttatttac agatacaaag aaatgatttt
     4441 tttaggaggg gtacgtttat ggcaaaaaaa gaaaaagaat tattatcaca attgacttca
     4501 gaaggtacca aaggaccgtt tgtcacgatt atgttaaaca cccatgttgc gcatcaagac
     4561 gtagaaaaag atcaaatcaa attcaagaat tttgcaaagg aagcgaaaaa gcgctttgaa
     4621 aagaaatact ctgagcatga ttggtctcca ttccaagaaa aaattactag tttgttggac
     4681 gatccgtctt tctggcgtag tgccacaacg agtgtagctg tcatagtatc accagaacaa
     4741 gtgtttatcc accgtttggg tattcctgta gatgaccagt attatgtgag tgatctacct
     4801 tatttattag cgatcatcaa aaatggacat ttcaactatt catactattt attaggattg
     4861 aatcgtgatt cgatgaaatt gtacgaagta acgaataaac aagtcaaaga agtggctttg
     4921 cctgaagatg caccaagaga tgtcgtcact gctttaggat ctgaactaac aggaggcggg
     4981 ttgaactacg catcaaatgg tagtgaaggt acgacgttcc atggagtcaa tcctaaagat
     5041 gaagaagtcg aaattgattg ggtcaattat tatcaagcag tcgatgacta tttgaaagat
     5101 acatttgaaa atccagagaa atggcctatc ttcttatttg cattaccaga aaatcagacg
     5161 atgttcaaaa aagtggcgaa gaacccttat tatcgttccg atgttgcgat ctcagcttca
     5221 cctgcacaac tttcattaaa agaaattgaa gcaggcgcaa aaaaactatc gcaagaatta
     5281 gctgtcaaag aagcaagtga ctacaataag ttgcttgata aaaagttcat tgatcaactc
     5341 gttgatatta taccggcagc aaaagaaggg aaaatatccc atttattcat tgctacatct
     5401 aatttagcag atggttttgg cgaagatcca gaaacagaat atgaccgccg tcaggtgctg
     5461 aatgaactag cagataatgt gatcacgaac ggtggtcagg tctttatcct tgaacaaaaa
     5521 gatgcaccag acgaaaagag tttagttgcg atcttacgtt attgagtgaa aactaccaaa
     5581 gtagatgaat ggtcaggcgt tgatagaagt gtatagctcc gagagaagga gtttcctctg
     5641 tttcagccgt tggattcatc ttgagcgaga gacaaaagct agcttttgtc tctcgctcat
     5701 tatttttgtg ctttagtccc attggctttg cgaggggaag atacttttct caaaaaaacg
     5761 aaccttgcag ttgagagggg gatagatgcg catactaatt tttatcagtt aaaaaatgat
     5821 gtggatcatt agagaattcc aaagttcgtt catccttttt cagataataa attgaaattt
     5881 tttgcaaaag tgaaaatagc aaatagaaat tttctgaatc ttataggggt gatttcgaga
     5941 aaacgttcgt ttatttgcaa atatatcttc taaataagag aataaataca aaaaaataag
     6001 gaaaaactct ggcaattttg taaacggtat gatatagtaa gaatgtagtt tttgttcggt
     6061 aatctgtggt agcttgtttc aaataaacat cttccagata tacaccattc aagtaattgc
     6121 aaaatatgtg ttgatacaca aggaggattt ttattatgga aacaggtaca gtaaaatggt
     6181 ttaactcaga aaaaggattt ggattcatca ctgcagaaaa cggaaacgat gtattcgttc
     6241 atttctcagc tatccaaggc gacggattca aaactttaga agaaggccaa gcagtaactt
     6301 tcgacgttga agaaggtcaa cgcggacctc aagcaacaaa cgttactaaa gcttaattta
     6361 cctatattaa aaataaaagt ccagacattt atgtgtctgg gcttttttgt ctttttttcc
     6421 ttaaaaagcg atgagagtat ttttctgcac tttgtagtat aatttcaaaa gaacattaga
     6481 ggcaggagaa gaggaagata ttttgaaacc aagaacttat gcagtcgttg atatcgagac
     6541 gactgggacg aatccaaaag aagatcggat catccaattt ggctgcgtca tgattgaagc
     6601 tggtcggatc acttcacgtt tttcgatcga tattcatcca gggcggaaga tttcaaaaca
     6661 aatccagcat ttaacaggga tcacgaatca acgcgtccaa aaagcgcctt attttgaaga
     6721 tgtcgcccaa acgatttaca atcttttggc agacaccata tttgtcgcgc ataatattta
     6781 ttttgattat aattttttga accatgaact catgcgatgc ggacttccta gtttgaagat
     6841 accgggcatc gataccgtag aacttgcaca aattttcttg ccgacggaac caagttttcg
     6901 cttagctgat ttgtcagaaa gccttggctt tattcatgag aatcctcatc aggcggatag
     6961 tgatgccgaa gtgacaggtc agcttctttt actgatcgaa gaacgaatga ggaaattgcc
     7021 gatcatcaca ttagagcaga ttgcacgatt aagtagccat acaggaatgg atacgagtcg
     7081 tttcatttac cacgtgcttg aagaaatgaa agaaaacccg gagccccttg atctctcatt
     7141 agaaatcatc gatgggctag ctttgcagaa aaaagaagtg gagctattca catccgtcca
     7201 ttatggcgag cggacatatc caagaaaaaa acaagcaaaa gaaaaaatgt ttggtaaaac
     7261 actgatgtat cgaaaagaac agaacaggct gatgaatgct gtctacgacc atttcacaaa
     7321 agatgaatca aaagatttga tgatcgaagc agctacagga atgggcaaaa cgattggtta
     7381 tctgttgcct ttgagttatt tggctacacc tgaaaaacca gctgtcatca gcaccgtttc
     7441 gcttgttttg cagcagcaga tcatggaaaa agacattcca ttactgaatc aattgctaga
     7501 tcagccgata caacctgtga ttatcaaaag ctatcgccac tatattgatt tacagcgttt
     7561 taaaggtaca cttgtggaac cgccgggaca aaaacaatat gctttgtacc aaatggctgt
     7621 cttagtttgg ctaacacaaa cgaagaccgg tgatttggat gaattacatt taaccaatct
     7681 gaaccatgca ttctttgcag atgtcgcgca cagaggcact gggtttttag caaggaatca
     7741 gtcattttac aaacaagact ttgtgcgttt cttgcaaaag aaaatcaaac aaagcaattt
     7801 tttgatagtc aaccatgcat ttttagtaca agaaacacag cgaaaagaac cattgcttcc
     7861 agctagtcct tatttgttga ttgatgaagt gcatcatttg ccggaaattg ctgaaaaagt
     7921 aaatgacaaa cgactaagta ttaactactt ccataaacaa attcagcaac tggaagagcc
     7981 gaatcagtta tttgaccgca tacaaagtta tttgccgaag gaacatgaag ccttgcgctg
     8041 gttgtcgatt tatcgagaag aactctctgc actatgcgaa gcgcagattt gcgtggcaga
     8101 gggattaatg aacttagtaa gcagcaagca acctaacgga caatatccag aagaagtcat
     8161 gatgacaaaa gaaatgttgg atcagctgcc actggaatct ggaacagcaa tggatcatct
     8221 gcaacttttt tatcaagaga tccaacaact acaatcacag atgcgcttga cttttgagat
     8281 tatccaagaa agttggacca accagatgcg gatcgattta gcaagtttat atagcttatt
     8341 tgatcaatta gacaagcaat acttgctgat ggaccaatgg ctagaagact ggcgagatca
     8401 tttgatccat tggttcgttc ccgggaatac agcacagcag atgatttttc atctccttga
     8461 ttttcaagca gctatgttgc cgtcaactgt gtggtatcct cgatatgacc atattcttta
     8521 tattggagga acactgaaga tcggacctaa tcgacagtat ctctctaaac gacttggcat
     8581 tgaagcagca cctcttaaag tgattgcttc tccttatgat tatgagaaac aggcaagaat
     8641 ctatgtgccg aaagaagcac ctgcagtcca tcaagtcagc aatcaagcat atatcgatta
     8701 tttagaagca gcactcacag gattgattga acaagaagat cggccgatcc ttgttttatt
     8761 cacttctcat gatgttttgc aaaaggtata tcagaaaatg catttgtcta tgctagaaaa
     8821 aggacgtgaa attcttgctc aaggatttgg cgggagtcgg gaaaaacttt tgaaacgctt
     8881 cattttgtca gaaaaaagcg tcttatttgg tgctgacagc ttttgggaag gcgtagattt
     8941 acctggagat gctttgcaga ttgttgtagt cacacgttta cccttcgaca acccgttacg
     9001 tccgatggtc aaagctagga atgcttatct ggaatcagag gggatcaatc ctttttatca
     9061 ggaatccgtg cctaaagcag ctttgaagtt acgccaagca ttaggtcgtt tgataaggac
     9121 ggagacagat cgtggcgtat tacttatttt ggatcggaga ttagtcactg caaaatatgg
     9181 tgcacgaatc atccatgcat taccgaaaaa agtaccgata aaagagctag ctatagatga
     9241 aatctatcag gatatccatg aattcttaaa gaaaaatgaa gaaggaagtt aaaggtgccg
     9301 tcttttttca tcattaaaat ctgctataat gaaaaggaat gaagataaag gagcgacgac
     9361 ccttgaagaa aaaacagaca aaggaaaaaa cgattaatcg cgtgttgctt ggcttgatcg
     9421 tatttttatt agcggtcatc gtgatcagct cgatttttta cttgcgttcc agtcgtccgg
     9481 cggtccaggc gaaaaaagaa gcagtagaaa tcgcaaagaa atatgcaaat ctcgaggaag
     9541 ccgatcattt ttactggttt actcgaaaga aaacgtactt ttcattgacg ggtacgaatg
     9601 ataaaggaaa agaaatcgcc gttatcattc ctaaatctgg tgaaaaagta aaagtattgg
     9661 atcaaacaaa gggactgact gaaaacgaag caaaacagac aattgctgcg gcccatcctg
     9721 aaatccaaat cgaaaaagct gctttaggga tgtatgatga tcagcctgtg tgggaagtag
     9781 taggaaaaga aacaaatggg aaactgaatt attacctgat tggctttgaa tctggagatg
     9841 aagtcaagac aatcaaggga atttaaaagg taaaggaatg aatgggatgg agttatcaaa
     9901 aagaacaaaa cgattagaac cgtctgtcac tttggcagct gcggcaaagg ccaaggcatt
     9961 aaaagcaaaa ggcagagatg tgctgagttt gactgtggga gaaccggatt ttgcgacacc
    10021 agaaaacatc caagaagcag caatcgaagc aatccgcaat gggaaagcta gctattatac
    10081 accaacagca ggaatcccag agcttcgaca ggcaatcgtt gattatctga agaaaaacta
    10141 tggacttgtc tatgaacctt cacaaacagt cgtaacagac ggagcgaaat ttgctttgta
    10201 tacccttttc caaacgatcc ttgacccgca agatgaagtc atcattcctg tgccttactg
    10261 ggtcagttac ggtgagcaag taaaactggc agaaggactt cctgtctttg tgaaagggga
    10321 agaatccaat agctggaaag ttactgttga gcagctagaa caagcacgtt cagaaaaaac
    10381 aaaagccgta atcatcaatt ctccatcgaa tccgactgga atgatttata gcaagaatga
    10441 actgcaggca atcggtgaat gggcagtaaa aaatgatatt ttgatagttg ctgatgatat
    10501 ttatggccgt ttagtttata atggcaatga gtttactcca attgcaacga tttcagaagc
    10561 aatcaaaaat cagaccatca tcatcaatgg cgtgtctaaa acatatgcga tgacaggctg
    10621 gcgcattggt tatgcggtgg ggaattctga gatcatcaat ggaatgattg ctgttgcttc
    10681 ccagtcaacc agtaatccga cagcagtcag tcaatatgca gctgttgaag cattgaaagg
    10741 cgagcaagat acagtggaag aaatgcggaa cgcctttgaa gaacgcctga atacgttata
    10801 tccgcttgtg gcagaactgc cgggtgtttc tcttgaaaag ccacaaggag ctttttatct
    10861 ttttcctaat gtaaaagaaa cgctacgcat gtgtaaatat gaaaatgtca cacaatgggt
    10921 agaagatctt ttagaagaaa caggggtagc gcttgtgact ggcgaaggct ttggggcacc
    10981 agaaaatgtc cgcatgagtt atgccactga tcgcgctacg ttagaagaag cggtacgcag
    11041 aatcaagcaa tttattgaga gtaaaagtca aaattaatcc tgaagaaatt caaggaatat
    11101 aaaaggagag tcatacgtgg aacaaattca aatcatcgat tcgaaaaacc atgtgggaga
    11161 aacagtaaca atcggcgctt gggtcgcaaa taagcgatca agcggaaaaa tcgcattttt
    11221 gcaattaaga gacggaacag cctttttcca aggtgtagtt gtaaaaagcg aagtgccgga
    11281 agaagttttc caaacagcta aaaatctaca ccaagaaaca tccgtgttag taactggaga
    11341 aatccgtgaa gacagtcgtt caaaatttgg ttatgaaatc ggaattacaa atgtggaagt
    11401 ggtaagcgag agccacgatt atccgatcac accaaaagaa cacggaacag acttcttgat
    11461 ggatcatcgt catttatggc tgcgttcttc aagacaacat gcgattatgc agatcagaaa
    11521 tgaaatcatt cgtgcaacgt atgaattctt taatcaacat cattttgtca aaatcgatcc
    11581 gccgatcttg actggttctg caccagaagg cactacggat ctattcgaaa caaattactt
    11641 tgatcaaaaa gcttatttat ctcaaagtgg ccaattatac atggaagctg ctgctatggc
    11701 tttcggaaaa gtcttttcat tcggtccgac tttccgtgct gaaaaatcaa aaacacgacg
    11761 tcatttgatc gaattctgga tgatcgaacc agaaatggct ttcactcatc aagaagaaag
    11821 tttagaagta caggaacaat atgtagcatt cttagtacaa aatgttttag atcattgcga
    11881 atacgcatta gatgtactag gacgagacaa agaagtctta aaaacttata caaaattgcc
    11941 attccctcgt attacttatg atgatgcgat cgaattactg aagaaaaatg gatttgatga
    12001 catcgaatgg ggcgaagact ttggttcacc tcatgaaacg ttcattgcga attcattcga
    12061 acagcctgtc tttatcctaa actatccaaa agccatcaag ccattctaca tgaaaccaca
    12121 tccaacaaga gaagacgtgg tgatctgtgc agatatgatc gcaccagaag gttacggtga
    12181 aattattggt ggaagcgaac gggcaacaga ctatgatttc ttgctagaac aaatccgtaa
    12241 tcacggtttg gatgaaaaag aatattcatg gtatcttgat ctacgtaaat acggttctgt
    12301 tccgcactct ggttttggtc taggccttga acgaacagtc acttggattg ctggtatcga
    12361 ccatgtgcgt gaagctattc cattcccacg tttgttgaac cgaatttatc cataagaggt
    12421 tgtgaaatca gtgccttgac ctgagtatta gagaaacaaa acggaaaaat agcttctcat
    12481 attttactgt tttttgagct aatatcgagg gtcagctgat tgaacaccgt ttatatcaag
    12541 aggttgtgcg tctgccgttc agctccgagt cacaaggagt aatttcaaaa aaacattttt
    12601 tcatgtttga tgactattgt gacttgtgac caaggagttg gccggagagc aacgtttatt
    12661 taaaaaaaag aactgcgaac tctcgcagtt cttttttttt aaatacggga taaaggaagt
    12721 ttccagcaga taagagaaga aagcaaaaga aattgctatt tgattcacaa ggttctataa
    12781 cgaaatcgca atcaatcaaa catatacaaa gaaggttatt caaatggaaa tctgatttga
    12841 tgatgcttct gctgttgaaa taaagcagca tctagcacct gaaaacaaac taatagtaac
    12901 attcgaagat ggagtaggtc cgtattttca gcatgcaatg atccatatgt aagttcaatt
    12961 cacccttgaa atcatcggac cagataaagt tatggccaat ctatcccaag caatctagaa
    13021 gagatattag taaaggaaca tttactggaa gtcttagatg agcctataaa tatatgttct
    13081 gatggcgtca tcaagtggtc tgattgacgg aagcttaggt gtgatcgatc tctctggacc
    13141 aaacaatgaa ggaatcaagg ggaatccagc acgttaagaa aaaaatagca taaaaaacta
    13201 gacacttgat gagcgttcat tcaattgttt gaagggacta ggctggtgtc attttttata
    13261 catatatttt ctctttcgtc ttgatagggc ttatgggata atataaggaa gaaaatcatc
    13321 gttttattca aaaaggagtt tttgacaaat gaaaaaacgt atattattta tcggtgacag
    13381 cattacagac gcaggaagag accgagcaga agcaactgat ctaggtaaag gttatccact
    13441 tttgattgcc ggaaaactaa tctcccaata tccaacgatg gaatttgaat gcatgaaccg
    13501 tgggatagga ggagacaaaa ttgcagatct gcaagtacgc tggcgagaag attgtttagc
    13561 tttacaacct gactatgtat cgattttgat cggaatcaat gatacctggc ataatactag
    13621 cgatcaggct atattcggaa ctgagaaatc tgctcagcaa tttgaatccg tttatcgaaa
    13681 attactaaat gaacttcaaa aagcgggaat ctgcaacatc atcttgatgg agccgtttgt
    13741 acttcctata accgacgacc gtcatggctg gcgtaaagac ttagacccta aaatccaaat
    13801 tgttcgtaaa cttgctaagg aatatggcgc attgctggtt cctttagatg gcatattcaa
    13861 tgctttaggg attgcttatg gtttttcgaa atatacaaga gaagatgggg tccacccaac
    13921 gattggcgga catgagatca ttgcacaaag ctggatagat gcaattgccg cagtcgattt
    13981 ttttagtgga gataaaaatg agcgaaagaa aagtgagaaa tgatggaaat acggtgggca
    14041 gtaaaagaag atctacctca aatcaatcag ctgattactg atcatccaga atcattcgta
    14101 aaagcaaaaa cggcagctga cctaaatgat aagaataaac tattgctcgt tgctgtaaca
    14161 aaagaaaaac ggattatcgg ttttgcgcaa gcggacgtgc aacagtcaga agaacgggtg
    14221 ggtcagcgac catcaggaaa actaatccaa cacacattag taacaaaaga aaatacggaa
    14281 gaaatccaaa aaaaatttgc agaatatttg atgctggaag ctaaaaaaag aagaataaaa
    14341 tcaatttttg cccagtttta atcaaaaaca gtaatagaag gagaaataca atgagttgtt
    14401 taggaaatgt catctggttt atattcggcg gactaatcgg tggtcttagc tggttagcag
    14461 cggggatact ttggtgtatc acgatcatag gtatcccaat cggtatgcaa tgttttaaga
    14521 tcgccttcct ttcttttgcg ccttttggca aagaagtaat ttatcaccag tctggcatgt
    14581 cattgatcgt taatatcatc tggctgatcg tttcaggcct accaattgct ctcgcacatg
    14641 tcgtcagcgg catccttcta tgtatcacga tcatagggat accttttgcc aagcaatctt
    14701 tcaaacttgc tcgattggcg ttgatgccat tcggtgcaga aattgtataa aagagaataa
    14761 taaaggttag ataaagcata gagtcaggtg ggatacatgt atttcacctg actctatgcg
    14821 ttttcctgta taagttcttc catgtgactt ttacgtcttt tatttatatg gggttactgt
    14881 ttatggaaag ttgaaataat tttcattaaa gaactctttc tatatttttg aaagcgtagt
    14941 ggagcttcgt atatctggga aggacatttt gcagggattg aaaaaagata ttgtcctatc
    15001 gaaaaattaa tgaatttaga caaattgcca gcaacaggct tcaaacttag ttgctatcct
    15061 gtcaagatca agaaggcaag tgcgggttgg ataagagcag tggcaatgat cgaagagaaa
    15121 aagaaagaat aagccaaaac atctgtatgg aagaaatttt ccgatgcaga tgtttttcat
    15181 tttttctttc ggataccttg acatatgaat aaccattcat ttataattag ttatgaagag
    15241 atattcatat gttgaggcga ggaggtcatt aaaatgaaaa gttacagatt agaaggttta
    15301 gattgtgcga actgtgcaat gaaaatcgaa aaaggagttc agaaaatcaa tggagtcaag
    15361 gaagcaacgg taaacttcac ttctggaaaa ctaacgattg acgcggagga agatcatttg
    15421 gctacgattg aacaagaaac aaaaaaagta gtgaaggaat tagaaccaga tgtcaaagta
    15481 acagaaatag ataaggaaaa agtatctgaa catggaaatg aaaaagagag aaatacgctg
    15541 ttccggattt tattttccct tgcaggtatc gctttattgc tattatttga ttttaatgaa
    15601 ctaattcgct tgatagggta tttattgatt tatctgctga taggatatga tgtagtgaag
    15661 aaggcagtga tgaatatcgt caaaggaaaa atctttgatg agaattttct tatgtctgtt
    15721 gctacgattg gcgctatgtt gatcggtgaa tatccagaag ccgtagcagt gatgctattt
    15781 tatcaaatcg gcgagtattt ccaaggattg gcagtcagtc attcaagaaa atcgattcgc
    15841 gaattgatgg cgatccgtcc agaaacagct cacgtgcaga cggcggaagg attgatgacc
    15901 gtgaaccctg aagacgtgct gatcggtcag tttgtacttg tgaagcctgg tgaacgtgta
    15961 ccgttagatg gaacgatcat cgaaggagaa tcattagtcg atacgtcagc acttacagga
    16021 gaatcagtcc ctaaatctgt atatgttggt gaaacagtat taagcggatt tatcaataaa
    16081 aataaaccat ttttagttca agtagaaaaa tcttatgaaa attctactat cagcaaatta
    16141 ttagaattag tagaaaatgc cagcagtaaa aaagcaccag ctgaaaattt cattactaaa
    16201 ttcgcgcgct attacacacc agtcgttgta ggattggccg ttttattagc tgttttacct
    16261 ccacttgttg tttctggagc ggcgttttct gaatggatct atcgagcatt gacattttta
    16321 gtgatctcat gtccatgtgc attagtgatt tccgtgccac ttagtttctt tggcggtatc
    16381 ggaggagcaa gtaaaatcgg tgtattagta aaaggttcaa attacttgga gttgcttgcg
    16441 caaaccgaga cagtggtatt cgataaaacc ggcacattaa cgaaaggcga cttttcgatc
    16501 cagacgatcg atacaaaaat cgatccaaag atattcatgc aatatattgc gagtgcagaa
    16561 cagttttcta cacatccgat tgctcaatct gttttagaag gctacgcagg tccgctttta
    16621 ccaacagcta atatccagga atttgcaggt gaagggatct tagctgaagt tgatggcaaa
    16681 caagtattag taggtaatca caaattgatg gaaagatttg aaatctcttt tccttcaagt
    16741 caagaaatcg gtacattgct ttatctagca atcgatcaaa gttacagtgg ttatcttgtt
    16801 attgcagata ccttgaaaga agatgccgta gacgcattgg ttcaattaaa acaggctggt
    16861 gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa ttgctgatca tatcgggaag
    16921 caagtaggcg tggacaagat atatagcgaa ttgcttccag aagataaagt gcagcggtta
    16981 gaggaaatct tacaaagtaa caataaaaag accgcatttg tcggagatgg aatcaacgat
    17041 gcaccggtgc tagcaagagc agatgtagga atcgcaatgg gtggattggg aagcgatgct
    17101 gcaattgaag cagcagatgt agtcatcatg aatgatcaac catcgaagat tgcagaagca
    17161 atccatttag caaagaaaac attgaaaatc gtcaaacaaa acattgtctt tgctattggg
    17221 ataaaaattt tggtactttc cttaggtgct tttggttttg catcaatggg agatgcagta
    17281 tttgcagatg ttggggtgac agtattagct gttttgaatg ccatgcgaag cttgcatgta
    17341 aaaaataaac gttgatcaat gaagaggacg aatgaacgtc ctcttttttt tatatccttt
    17401 tcttaatgaa aaatgtaaag aaaatagata ggattataaa ataactaatg tttttaattt
    17461 tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa tgaaataaaa gaaaataact
    17521 tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat taaataaaat aatacacaca
    17581 taaaataaca ctgtcttgta agtttgttaa aaatgaatat gattacatct ggataacaaa
    17641 tttttatcat aaaggagaga acgaatgaac aacaaacaat tacgctggtt tactgttgcg
    17701 ttaattgcat ttaacatggt ttggggcatg gggaatgtag ttaataacta tgctcagcaa
    17761 ggagtatcag ttgtcacctc ttggctattg attttggcca tttattttat tccatacgca
    17821 ttgatcgttg gacaattagg atcagccttc aaggatagta aaggaggcgt cagttcatgg
    17881 gtcgaaaata cgacatctaa taaacgactg gcatactatg ccgcttggac ttactgggtc
    17941 gtccatattc cttatttggc acaaaagcct caagcaattc tgattgctgg tggttgggca
    18001 gtccaaggaa atggaaatat cgtaaatacg atgtcagtac aaatggtagc agttatttca
    18061 cttattatat ttcttgcctt tttatattta tcaacaaaag gactttctac tttgaaagtg
    18121 attggcggac tggccggaac agcgatgttc gtcatgtcga tcttatttat tcttttagct
    18181 gtaacggcac cgttcatcaa tccaacgatg gaatttgcca caccacatat gaatcaagtg
    18241 aaaacatata ttccaacatt tgatttcagt tatttcacaa ccgtttcgat gctagtattt
    18301 gcagttggag gggcggaaaa aatatcccct tatgtcaact ctacaagaaa tcctgcaaag
    18361 gaatttccta aagggatgat cttcttagca gcaatggtag ggatttgtgc tgtattaggc
    18421 tcagtcgcaa tgggtatgct gtttgctagc gataatatcc ctaaagactt gatggcaaat
    18481 ggggcataca gtgcttttca aattttaggt aattactatg gtatcggtaa ctcattgatg
    18541 atcatttacg cattgacaaa catgattgga caaatctccg cattagcctt ttcaattgat
    18601 gcaccactaa aaatcttgtt agcagatgcg gatccagaat ttgtaccaaa ctggctacgt
    18661 aaacgtaatc gtaaaggtac attggtgaat ggttatcttc tgacaggtat actagtagga
    18721 atcattattt tgttgccgat ctttggtatc aaagatatga atgaattagt aaaatggttg
    18781 acgaacttga actcagttgt catgccaatg cgttacctat gggtgttctt ggcatatatg
    18841 atgttgaata aagcttataa gaaattcaat tctgaatata aatttgttaa aaatccaaaa
    18901 atcggtttta tctttggtgc ttggtgcttt gccttcactg cgtttgcatg tatcctagga
    18961 atggtgccaa aagtagatta tactgctgat cctaaagcat ggtggttcca attattcacg
    19021 aatatcatca caccaattgt cttgatctta ttaggtatga tcttgccagc aattgctcgt
    19081 cgagacaaaa acaaagaaat tactgaacca gaaacagtgg ttcagcctta aaatagaaat
    19141 acttgttaca agaggttgtg acagaagcta cttctggaac accgtgaatc agaggttgtg
    19201 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcatatttt
    19261 gccgtttttt gagctaatgc tgaaggtcag ctgattgaac accatttatt cgaaacaagg
    19321 gactttgaca cgacttttgt cacagtccct tgtttttttg cggcgttata agtggtatga
    19381 tcgaaccaaa ttccaaagag aagtgaggga gaagaatgaa gatcatcgtg attggacaaa
    19441 gtgggtcggg aaaatcgtcc ctcgcaagga agatcaaaga aatcaccggc tttccgcatt
    19501 caggcaaaaa agtggttttt gcaagaacag caatcgttta tagtatctaa tccgtcatgg
    19561 atcgttgaag gaaattatac ttctacgtta gaggaaagga ttaaagaagc agataagatc
    19621 atttggctta aagtgccgcg ttatctcgcc atctaccgag tagtttggcg ttctctaaaa
    19681 agaaagataa acaaaaaatc tcggccagat atgcctgaat cattttcgga gaaactaaac
    19741 aggaaatact tagaattttt atcttttatc tggcattttg aaggaaacaa tgaaccaaaa
    19801 atacagcagc tgatccaagt agctaatgca agaaataaac tgatcatcct taaaacaaga
    19861 cgagagaaac aagaattttt aactaaattg aaaatagaga gcaaaaataa ttgacagttt
    19921 ttgtttacaa gcgtaatctg tattcataag ctaatgatta catttgtaaa cgaaaggtgt
    19981 gaaataaatg gaaaaagtca aaacgccagt aaagatcagt gattcagagt gggagatcat
    20041 gcgtgttatt tggacacttg gacagacgac agcccaagaa atcacgcaga tcttagctga
    20101 ttcgatggat tggaaaccag caacgatcaa aactttgctg ggacgtttgg taaaaaaaga
    20161 agtgatctgg acagaacaaa aaggaaaaaa attcatttat catccagctg tctcggagat
    20221 ggaaacagtt cgcagtgcga cggaaaatct tttttcccat atctgtgcaa aaaggattgg
    20281 agagacgatt gcggatctag tagaagaagc tactttgact caagaagata tcaatgagat
    20341 tttgaaacaa ctggaacaaa agcaaccagt agatacgatc gaatgtaact gtatcccagg
    20401 tcaatgtgaa tgtaaacaac atcaatagga ggaagaaaaa atgaaacaac aattttcaat
    20461 cgaaggtatg agctgcaatc attgtgcagc aagagtagaa gaaacagtaa gcgcacttga
    20521 cggcgtgcaa aaagtgaaag taaatttgaa aaaagcaaac ggtacggtca agtttgatga
    20581 aacaaaagtt caatccgaaa agatttgtca agcaatcaat ggattgggtt ataaagcaga
    20641 ggtgatttga tggcaacagc agaaaccaaa atggatacgt tcgtcattac aggtatgact
    20701 tgtgcaaatt gttctgctag gatcgaaaaa gagctgaacg gacaacctgg tgtggtcaat
    20761 gccacagtca acttggccac tgaaaaagca agtgtcaaat atgaagggac gaccactgaa
    20821 aaattaatcc aaagcgtaga aaacatcggt tatggtgcaa ttttgtacga tgaagcccac
    20881 aaacaaaaaa ttgcagaaga aaagcaagcg tatttgaaaa aaatgctatt cgatcttata
    20941 ttgagtacgg ttttgacttt gccgttgatg ttgtcaatga ttgctatgat gttaggaagc
    21001 catgcggcga ttgttcactt ttttcatttt ccaatcgtcc agttagtgct gtctgctcct
    21061 gtccaatttt atgtcggtgc tcgtttttat aaaggtgcgt accatgcaat caagacgaaa
    21121 gcaccaaata tggatgtatt ggtagcaatt ggaacatcgg ctgcctttgc attgagcatt
    21181 tacaacggat ttttccgtgg acacccccaa gatctttatt ttgaaagcag cagtatgatc
    21241 atcacgctga ttttgctcgg caaatatttg gaacatacag caaaaacgaa aacaggtaat
    21301 gcaatcaaac aattgatgtc tcttcaaaca aaaaccgccc aagtgataag aaacgggaaa
    21361 gaagaaacgt tagcgattga agaagtagtc gttggtgatc aactagtcat ccgtcctggt
    21421 gagcagattc cagcagatgg tcgaattatt tcaggaagca gtgcgataga tgaaagtatg
    21481 cttactggtg aaaatcttcc tgtagaaaag aatccggatg acacgctttt tggcgggaca
    21541 atcaatacga atggtctgct acacatggaa gtcacacaag tcggcaagca gacagtatta
    21601 gcgcaaatca ttcaaatggt ggaagatgct caagggagca aagccccaat ccaaaaaatc
    21661 gcagatcgga tctcagggat ttttgttccg atcgtattag tcatcgcgtt tatcacgctc
    21721 atagctacag gtttgatcac tggtgattgg caactcgcat tgatccatag tgtctctgta
    21781 ttagtcattg cgtgtccatg tgcattaggt ttagcgaccc caacagctat tatggtagga
    21841 acgggtgttg gtgcacgaaa tggaatttta attaaaggcg gagaagcatt agaagcagct
    21901 gctcacttag acagcattgt tttggataaa actggaacga ttacagaagg aaagcccaaa
    21961 gttacagatc tagtcggttc aaaagaagtc ctatctattt tttatacttt agaacaagct
    22021 tctgaacatc cactaggtaa agcaatcgtg gaatacggaa aactgcaaga agcagcaaca
    22081 tacgatatga ttgattttac agcccaccca ggtgctggga tcagcggtac gattaatgga
    22141 gtacgctatt ttgcgggtac acgtaaacga ttaattgaat tgaacctatc atttgatgaa
    22201 taccaagaac acgcgttaga attagaacag cagggaaaaa cagtcatgtt tttagctgat
    22261 gaaaagcaag tgatcgggtg gattgccgtg gctgatcaaa tcaaactaga agccaaacaa
    22321 gcaatcaagc agctccagaa taaaggatta gatgttttta tgctgacagg agacaacaaa
    22381 ctggcagccg aaacgattgg taaacaagtt ggtattgatc caaagcatat ctttgctgaa
    22441 gtacttccag aggataaagc agcctacgta gaaaaacttc agaaagatgg caaaaaagtc
    22501 ggtatggcag gcgatggaat caatgatgca ccggccttag cattagctga tgtcggtatg
    22561 gcaatgggaa gtggaacgga cattgcgatg gaaacagccg atgtgacgct gatgaatagc
    22621 agtttggcat ccattgcaca aacaatcgag ttgtctaggt tgacattgag aaaaatcaaa
    22681 caaaatcttt tctgggcttt tgtctataat acgattggta ttccttttgc cgcactaggc
    22741 tttttgaatc caatcattgc aggaggagcg atggcgttta gttccgtcag tgtattgttg
    22801 aactccctga gcttaaatcg acacatgaaa aagtaagaga agggatggag taaaaatgaa
    22861 tgggaacgat atggagaaaa aacatgagca taaaaaagaa gtagtaaaca aagaaagtac
    22921 agaatctcat gaaaaacaga tggagatgaa acatgaccat agtcagatgg atcattccat
    22981 gcacatggga cataaccatg gagatatagc aaacagcaaa caaatggacc attcgatgca
    23041 catggatcac gaacacggcg gcatggatca ttccatgcac atggggaatt tcaaacaaaa
    23101 attttggcta tcgcttatcc tagctatccc aatcattctt ttttcaccaa tgatgggaat
    23161 ggaatttcca tttcaagtaa cgtttcctgg ttctgattgg ctcgtattga tcttagcaac
    23221 tatactattt atctatggcg gtcagccatt tttaagtgga gctaagatgg aattgaaaca
    23281 aaaaagtcct gctatgatga cactgatcgc gatggggatt acagttgcat atatttatag
    23341 tgtctattcg tttatcgcaa atctgctcaa tccacataca cacgtcatgg atttcttctg
    23401 ggaattggct actttgatcg ttattatgct actaggtcac tggattgaaa tgaatgctgt
    23461 gtcgaatgcc agcaatgctt tacaaaaact agcagaatta ctgccagaat cagtgaaacg
    23521 cttgacgaaa gatggaaaag aagagacagt ttcattgaaa gaagtaaacg aaggtgatcg
    23581 tttgatcgtc cggtctggtg ataaaatgcc aacagatggt gtaatcttga aaggtgaaac
    23641 aattgttgac gaatcagctg tgactggcga atcaagagga atcaaaaaac aagccaatga
    23701 caaagtgatt ggcggttcta tcaatgggga tggaacgatc gaaatcgaag taactggaac
    23761 tggtgaaaat ggttatctag cgaaggttat ggaaatggtg cgaaaagcac aaggagaaaa
    23821 atccaagcta gagtcattat ctgacaaagt ggcaaaatgg ctgttttatg tcgcattaat
    23881 tgtgggtatc ctggcattca tcgcctggtt gttcttgaca gatcttccta atgcattaga
    23941 acgtatggta acagtcttta tcatcgcctg tcctcatgct ctaggtttag ctatcccatt
    24001 agttgttgct cgctccacat caatagcagc taaaaacgga ttacttttga aaaaccgcaa
    24061 tgcactggag caagccaatg atctagatgt gatcatgctg gacaaaacag gaacactaac
    24121 agaaggaaaa tttactgtaa cgggtgtcga agtacttgat gatgcgttca ataaaaatga
    24181 aatccttcaa tatctaggag ccttggaagc gaatgccaat catccattgg ctgttggcat
    24241 tatgaattat ttgaaagaac atgaaatcaa gccttatcaa gctgaaaatc taaaaaatct
    24301 ttcaggagtc gggctagagg caacagtcaa aaaacaacaa gtgaaaatag ttaatgaaaa
    24361 agaagttgag cgtctcggat taaacgtaga acaagcatta ttgaaacctt accaagaaca
    24421 aggaaatacc atcagcttcc tgattttaga aaatcatcta gctgcaatcg ttgctttagg
    24481 tgacgtagta aaaacagaag caaaagaatt tatccgaaca ttgaaagaaa gaaaaatcac
    24541 tcctgtcatg cttactggtg acaataaaaa cgctgcccaa gcagtagcag attatttagg
    24601 gatcgaagaa tattatggcg gtttattacc agatgataaa gaagctgttg tccagaaata
    24661 ccttgaccaa gggaaaaaag taatcatggt gggtgatggg atcaatgatg cgccaagttt
    24721 agcccgtgct tcaatcggga tggcaatcgg tgcagggaca gatatcgcaa tcgattctgc
    24781 ggatgtcgta ttgacaaaca gcgatccaaa agatattttg cattttcttg atctagccaa
    24841 gcaaacgcga aagaaaatga tccagaacct ctggtggggg gcaggttaca atattcttgc
    24901 aattccgcta gcagccggaa ttcttgcgcc aattggtatc atattaagtc cagcagttgg
    24961 tgctgtcttg atgtcgttaa gtacggtagt tgtagctcta aatgctttga cattgaaaat
    25021 caaataataa tgaaaaaagg ttgtgacaaa aaacgtgtca caaccttttt ttcgagtaat
    25081 cgatactcaa tcagctggtt ctcggcattc cagaagtaga ttctccagaa attttatctt
    25141 attttttgaa gcggctccct tttgtcagaa tttttttgta taccgttttt cttattatac
    25201 gcgttatttc taaataggtt tttttatcga attttaccgg atctgcttgt cgtccatata
    25261 tttctgcttc atattgagta gttaatcgaa tgaaacgcag atctagcttg aattcttgtt
    25321 ctattcgcag gctatattcc tgcaaagatt ctgcgggttt gcgcggtacc aatgtttcca
    25381 aagggttaag aagtttttga tatgcaatcg tattagtaga agcaaaaaaa tgaagataga
    25441 taagataact tgtccagcgc agagattttc tgaataacaa tagaaagaac aacaagacgg
    25501 ataaacacac tccaaggctg aataagacga ttttataagc tggtgtgagt gttgtttctt
    25561 ctgatggaat gacctcagat ctctcactgg aaatagtttc tgccatattt tcgctttcat
    25621 ttggttgttc gatagcaggc tggacggttt cggttgtttc agcaactggt tgtgaaccca
    25681 ttggattggc aaatgaaggg gttggttcaa atggaagcca gccaaatcca ggaaagtata
    25741 cctctggcca agaatgagca tgactattga gaatgctgta ttcctcattc ccttgattat
    25801 ctgtgcctgt tctgagccca tttgtaaaac cttttgtcca gcgagctggg ataccaagtg
    25861 ttcgaagaag gacgaccata gaagttgaaa agttgtcaca atagccgact tttgaatcaa
    25921 agaggaaata atccacataa tcgtgttctt tcggagtatg cccggtatcc gttttggaat
    25981 agcggaaatt tccgcttgtt tttaaatact gttagacagc ttcgacttta cgaattaaag
    26041 ttgcttcatt tttggtcaat tcgaccgcca agtcctcaat acgtttaggc aaactttttg
    26101 gcaattgaag atttgtctgc tgattttcta aatcatcagc ttttaactga ctgcttttta
    26161 attcttctct cgtaaaaact aacctttctg tttgtaaagt tgcagaacgt gtcgattcat
    26221 tcgggacagg aatgaatcgc tggctttcag gcaaatagta aagcgtttgt ccactagcta
    26281 atgaaaaatc agtcaaggta gtatttccat aaggataagg taagaaagct tgttttttta
    26341 gggtcaatgt aatgttttct ttgtcttcta taacggtttg gtagctcgtg atgtcaacag
    26401 ggaattcttg gattggttgg atgggtgcgt cctgatcttc tatccatcca gaaccggtgt
    26461 aatgattttt attttcgatc ttccaatagc catttccttt ttggttcgct gtaaaaacaa
    26521 cttcgccatt atcatataaa ggtccaccaa gctgttcatc attctcactg aagcctgttc
    26581 ttgcacttcc ggttaattga tactcatgga gtgtgtcata aagcccttca tcattaagat
    26641 attctcggat cggttcactc gtttcctcga gagattggtg tactttcgga agatataaag
    26701 gcaagatagc gctaaaagtt aagaaacaaa taagcagaac tgtagtcaat gactggaatc
    26761 gagtgtttcc cgacaacaac cggatggaga aaaattgcct tgctgccaga tacacgaaca
    26821 aaacagttac taccgcaatc atttcgtaca gtaaatcttt tttccgaaaa acggtaagca
    26881 tcatgaagta agccatcaaa gaaagaaagg gtgcgtacca atagtcgtaa tccacggtca
    26941 atgtaataaa taaatagatc agcaacatta tgagaaaaaa agctgtcttt tctggcaaat
    27001 aataaagttt accttgggat agatggaaga attcctctgt aactgacgaa aaaaactgta
    27061 agatccataa tatggaaaat gtcttcgaag gaaaatataa aaacaaacaa cctatcatta
    27121 ctggcaaaga aagcagcaaa cgtacccatt ttttaggaat acagcagaca ataaggcata
    27181 ggacaccaat agttaggata agaaggtagc tgttttgcaa ttgattagct actacaaata
    27241 tcggagcacc tacacttagt atcacaaagg agtaaacgcc acgcagccat ttttcccgtt
    27301 taatcgtcaa tccattcacc tcctggcaat gaacatatct tgtctttttc ttggaccatg
    27361 agttgttttt ctacgaaata aacaagataa gccgttcgat cttttgacaa tgtttcaata
    27421 gcttctgaga cttcaggaga attctcagga acaaagagga cgatttgttt ttttgcatat
    27481 tttttcaagt gtgcgaaaaa agctttctca tcgccagctt ccaaagaagc aaaataggta
    27541 tgaccggggt ctttgccatg aaaggcgcgg ttgcctaata tcagaagttc cggctgcttt
    27601 tcttttctca aatgccataa agaaaaatac aagtctagca gaggttcaaa ttttggactg
    27661 ggcgaacccc aaaagcaaaa aagaggagaa cattcttctt cttgataatc gtattcacgg
    27721 tataaccact cttgagtatg agcagacaat ttccaatcga ttcggttgaa agaatcacct
    27781 ggacgataag cgcgatgttc ccgaaaatca agacctcttt ggtacgcgtt cagctgctgt
    27841 tttttttcca gaactctctg taatttttct gcttgaatct tacaaaaagc aggcagaaca
    27901 gtcacaggaa aagagatctc tctttttgca ctttttctga aaagtcctag actatcatag
    27961 gctgtaaaaa tgacagttaa tgtttcatgg catcctcttt cttttggctg ccaaagcaat
    28021 tggacttctt gcggtttatt tgtaagaatc aaaatctctt gtttttttct gcaaagattg
    28081 ctgggaagcc gatttgtaaa cgagggagag gtaaagaaaa agttctcttt tttgctatgg
    28141 aaaaaataac ctgcatactt tttttgcgat aaacgagtgg ctgcaatgta caagtaaccg
    28201 aaagtttctt tagaaagctg cttaatgaaa tcaagctcca tgatacataa agagtaaaaa
    28261 aggccaacaa gaaccagcct aaagaattgt tgaatacaag agcaaaaagc acaatgatga
    28321 aatataccag aaataacaca aaattaatga aataatcttt gattttttcc attgtctacc
    28381 tcaatctacc tcacaggaat aggaatttgt gcaacaagag tatgaatgat ttgtttcttt
    28441 tcgtcttcct tcaatgaacg atcgtaaaaa atcaggcgat gggaaaacac gtaaggaact
    28501 aaatcctgca aatctttggg cgtgacgtaa tttctcccat ggattaaagc ataagctttt
    28561 gctgcctgaa taaaggaaag gcttcctcgt ggactgatgc caagctggat ggcagcatga
    28621 cttcttgttg ctgacactaa ctgcaaggca taagatgcca cttcgggact gacaaagaca
    28681 ttcgccacat ttgtttttat ttcttctatt tcataactgt tcaatacttc atttaagtta
    28741 tccaacactt tttcgaattt atccaaaagt agcaaaagtt cgtcatcaaa agaaggataa
    28801 ccgatttgta gtcggaataa aaaacgatcg agctgtgctt ccggcaaagg atatgttcct
    28861 tcgtactcga ttggattttg cgtagctaag acaaaaaaat gattgtctag agggtatgta
    28921 ttgttgtcga tcgttgcatg attctcggcc attgcttcta aaagtgctga ctgagtacga
    28981 ggagtcgtac gattgatctc atctgccaac aagatcgtgg taaagatcgg tcccggacga
    29041 aattcaaact cttttgattg tgaattgtat acggaaaaac caagaatatc actaggcagc
    29101 agatcaggag tacattggac tctgctgaag gttccctgta cagcttttgc tagtgctttg
    29161 accagtaacg ttttacctac accgggaata tcttcgaata agacatgacc acctgctaat
    29221 agggccacga tcatcaattg gatagttgaa cgtttgccga tgaccgcttt ttctaattct
    29281 tatatcaact gatcgatttt ttgtttggat tcttctattt tttcttccat tttcacatca
    29341 ccttctcata aaatcgctat ttatttcatt gtaatggaag ggaatacatc ctacaagcaa
    29401 agtgatctta ttatgttttt ttaaagtaca ttcgttttga aatgagaata cgattaaatt
    29461 gagtaaaata aaaaataatg aggaagattt gcgtatttaa ataaatagaa agggagtttt
    29521 ttatcttttt aggagtgaac tcattttgat caaacaatat gtttagaaat caggtgagaa
    29581 atgatcgatg agatggcaaa aactattatc aaaaaatgca cagaatttat tttcactaat
    29641 caatctaatg aaagatttga tctggcgaaa agctgattta gctgaagaac tgaaaatcga
    29701 tgttaaaaca gtggatcggc atctgaaacg tttggcggaa agcgactgtc ccattcagct
    29761 gattcttcat aagaaaaccg ttcagttagt ttatgatcaa ggatatagcg aaccttatct
    29821 cttgtttgtt tttctgatcc aatcaccccc gttttgttta ctgaaaaatc tgctattaaa
    29881 tgaaccttac gagcagaagt acgatcgttc tacgcaagag cgattgagaa agtggtttgg
    29941 cgagtaccac ctttcttttt cttataaaaa agcacagatc tttggttcag aaagaaaagt
    30001 aagatatctg ctgttttact ttttgaaaga ttttccgcat ttttttgcag atgatggaga
    30061 gcagtccttt tctgagatgt tcatccaatt attgaagcaa agaaaggatc cggcagaaat
    30121 cgagatttta ggagacagtc gaacactatt ctttgaattg tttcctgaat tgctatttag
    30181 agaggagcag attttgccaa taaaagaaaa aaattatcta gcaattttgc gcttgagctg
    30241 taaccagatt actgaaaagc ggtggaaacg gatagaagag acacaagact atcggaaatt
    30301 gacgattgtg actgaaaagc ttgattcgct gttttggctg aatagcgaat ttgagagcga
    30361 aagcaaatca aagctgataa aagtacttta ccaagaatgg cttcgttacg taattggtat
    30421 ccatgaccgg ctgatagaag aagttcgtta ttatcaggaa acattagaaa aatttcccaa
    30481 ttccaaagat aaactatatc aatttcggct agcgatccaa caagcacttg cacaccccgc
    30541 agtagagtgg gggatgcttt tactgaaaac actccaggaa cgagggtatt tagatgtcca
    30601 ccctcctgaa gtgaaaatcg tctttttttt tcgctatgaa caccaagaag caagaagact
    30661 tgctggggta ctgaacaaaa cgctaagaca tcgaaaacgg atctctatac atgtatgtac
    30721 tcgaaataag ccgcctcggt atgcagatct agtgatcacc gatcattttt ttccactaga
    30781 ccataacgaa gatcaaaaaa cgtatctgta ccatccttca tttggtatcg aaagacttct
    30841 taccgatatc aattactggt taggcaaaaa ccgataaaac ctttggaaaa aagatcaaaa
    30901 caagtcagtc tgtgctataa taaaaagcga ttagtaaagg aggaaatcca ttggaattaa
    30961 agaaattaga agttcccaca tcttcgatca cagaagtgaa acgatcgccg atggatgtat
    31021 ttgctcaagc gcgagacgcg ggaactggcg tatatatatt taatcgtgaa aaagtagctg
    31081 gcgtgatgct gacccaagaa caatacaaaa cgctcttgca agaactagat gtccttcgtc
    31141 aaacagtgaa agtggagagg aaagaaccaa aagaaataaa cgaagaacct attaacaaaa
    31201 cagcttccgt cgtatccaat gctgaaggga tggaagagct ggcccaaaca ttacagcact
    31261 cgctcattac tggtgtgacg attacagcaa aaaatctaga tgaacgaatg gtagcactag
    31321 gttttattac gaaaaagacc ggatttggcg gagtcgtgga tatgcttagc gagttgatgg
    31381 aaactgggaa aatcaactac cagttaagaa gaaaaccgca aaatcggaca atcatagctg
    31441 aaatcatagg agagcaaaac agtcaatctc aattgatgga taaaatcatc atcaagaaaa
    31501 tttttctaca tgagctataa gaaaaccatg ttcgcacttc taggcaagcc aggctttgaa
    31561 gaccttgcca aagaattgaa cgaacgcctt taagataagg gtttttcgct agatttaaaa
    31621 aatcagtgat agaaacatgt tcgcttataa ataaaattag tgatagtttt agtgatagtg
    31681 agcttaaaaa agcactcaat ttgagtgctt ttttatttgc tcttctatat agttttctag
    31741 ttgattcatt gatctttttt tcacttcggg agaaatatgg gcatagactt gtgtagtgga
    31801 aacatcttta tgacctaaaa aatctttgat atcttctaaa ggtattcctg cttgtctcgt
    31861 tctagctgaa aaagcaaaac ataatgttgg tgttttcctt tcagcatatc tatcagcaag
    31921 atgtagagta ggacaacctc gtgagccaaa agtcctgtgc aaatcgagtt acaacacaca
    31981 catgataaag aaacttttga aaagaactat aaagtatacg ttgaattggc aagagattca
    32041 gcaatgaaat atggtattcc attaacatta gacactcctt ataaccaacc aggaatcaaa
    32101 tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatcctta cggttatctt
    32161 tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg ttttacggat
    32221 gataatccaa ctacttcgga ggataaacca gtaattgatc caactcgagc aggtgctgca
    32281 aattctacgc tgacagatgg aacaaattac gcccacattg atcagttcgg agaaatcgaa
    32341 aacgcaaact tgcatgtagc tggatggcac attgctaatt ataaatacga gtatattttc
    32401 attatggact acaatactgg aaaagaacta gctagagtaa gagctgatgg gatttataga
    32461 ccagacgtaa atcaagctta taatacttta ggaaatgttg gctatcatgt atctttcaat
    32521 atgcgtgatt tccctaataa gaaagtatac gtcatgatgc gtgcgacaaa cgatccagaa
    32581 ggaaatacta agggtggagc acaagatttt catgacaagc gttggtattt aaatattcca
    32641 caacgataaa aaatcccccc ctcttt
//
LOCUS       DAYYHS010000022        32626 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000022 DAYYHS010000000
VERSION     DAYYHS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.022"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACHA6A_001055"
     CDS             complement(29..1261)
                     /locus_tag="ACHA6A_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8969273.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACHA6A_001056"
     CDS             1516..2085
                     /locus_tag="ACHA6A_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8969274.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACHA6A_001057"
     CDS             complement(2263..2703)
                     /locus_tag="ACHA6A_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8969275.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACHA6A_001058"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACHA6A_001058"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8969276.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACHA6A_001059"
     CDS             3657..4580
                     /locus_tag="ACHA6A_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8969277.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACHA6A_001060"
     CDS             complement(4805..5044)
                     /locus_tag="ACHA6A_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969278.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACHA6A_001061"
     CDS             complement(5049..5468)
                     /locus_tag="ACHA6A_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8969279.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACHA6A_001062"
                     /gene_synonym="mefH"
     CDS             complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACHA6A_001062"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8969280.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="ACHA6A_001063"
     CDS             complement(7040..7675)
                     /locus_tag="ACHA6A_001063"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8969281.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7789..7881)
                     /locus_tag="ACHA6A_001064"
                     /pseudo
     CDS             complement(7789..7881)
                     /locus_tag="ACHA6A_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8017..8652)
                     /locus_tag="ACHA6A_001065"
     CDS             complement(8017..8652)
                     /locus_tag="ACHA6A_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8969282.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="ACHA6A_001066"
     CDS             9164..10306
                     /locus_tag="ACHA6A_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8969283.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="ACHA6A_001067"
     CDS             10299..10505
                     /locus_tag="ACHA6A_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8969284.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="ACHA6A_001068"
     CDS             10584..11777
                     /locus_tag="ACHA6A_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8969285.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="ACHA6A_001069"
     CDS             11982..13121
                     /locus_tag="ACHA6A_001069"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8969286.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="ACHA6A_001070"
     CDS             13114..13914
                     /locus_tag="ACHA6A_001070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8969287.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="ACHA6A_001071"
     CDS             13914..14741
                     /locus_tag="ACHA6A_001071"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8969288.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14746..15453
                     /locus_tag="ACHA6A_001072"
     CDS             14746..15453
                     /locus_tag="ACHA6A_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8969289.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="ACHA6A_001073"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="ACHA6A_001073"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8969290.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="ACHA6A_001074"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="ACHA6A_001074"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8969291.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="ACHA6A_001075"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="ACHA6A_001075"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8969292.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="ACHA6A_001076"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="ACHA6A_001076"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8969293.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="ACHA6A_001077"
     CDS             19038..21071
                     /locus_tag="ACHA6A_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969294.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="ACHA6A_001078"
     CDS             21115..22395
                     /locus_tag="ACHA6A_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8969295.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="ACHA6A_001079"
     CDS             22605..23411
                     /locus_tag="ACHA6A_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969296.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="ACHA6A_001080"
     CDS             23423..24643
                     /locus_tag="ACHA6A_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969297.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="ACHA6A_001081"
     CDS             24633..26219
                     /locus_tag="ACHA6A_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8969298.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="ACHA6A_001082"
     CDS             26258..28396
                     /locus_tag="ACHA6A_001082"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8969299.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="ACHA6A_001083"
     CDS             complement(28764..29747)
                     /locus_tag="ACHA6A_001083"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8969300.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="ACHA6A_001084"
     CDS             30125..31516
                     /locus_tag="ACHA6A_001084"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8969301.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="ACHA6A_001085"
     CDS             31532..32572
                     /locus_tag="ACHA6A_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8969302.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
BASE COUNT        10493 a         5677 c         6450 g        10006 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggt
//
LOCUS       DAYYHS010000023        31953 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000023 DAYYHS010000000
VERSION     DAYYHS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.023"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1060
                     /locus_tag="ACHA6A_001086"
     CDS             95..1060
                     /locus_tag="ACHA6A_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8969303.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1117..1347)
                     /locus_tag="ACHA6A_001087"
     CDS             complement(1117..1347)
                     /locus_tag="ACHA6A_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8969304.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1433..2320)
                     /locus_tag="ACHA6A_001088"
     CDS             complement(1433..2320)
                     /locus_tag="ACHA6A_001088"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8969305.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2409..3632)
                     /locus_tag="ACHA6A_001089"
     CDS             complement(2409..3632)
                     /locus_tag="ACHA6A_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8969306.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3649..4695)
                     /locus_tag="ACHA6A_001090"
     CDS             complement(3649..4695)
                     /locus_tag="ACHA6A_001090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8969307.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4859..6586)
                     /gene="ptsP"
                     /locus_tag="ACHA6A_001091"
     CDS             complement(4859..6586)
                     /gene="ptsP"
                     /locus_tag="ACHA6A_001091"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8969308.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6586..6852)
                     /locus_tag="ACHA6A_001092"
     CDS             complement(6586..6852)
                     /locus_tag="ACHA6A_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8969309.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7331..9565
                     /locus_tag="ACHA6A_001093"
     CDS             7331..9565
                     /locus_tag="ACHA6A_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8969310.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9713..10032
                     /locus_tag="ACHA6A_001094"
                     /pseudo
     CDS             9713..10032
                     /locus_tag="ACHA6A_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
     gene            complement(10237..10461)
                     /locus_tag="ACHA6A_001095"
     CDS             complement(10237..10461)
                     /locus_tag="ACHA6A_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8969311.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10659..12239)
                     /locus_tag="ACHA6A_001096"
     CDS             complement(10659..12239)
                     /locus_tag="ACHA6A_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8969312.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12640..14016)
                     /locus_tag="ACHA6A_001097"
     CDS             complement(12640..14016)
                     /locus_tag="ACHA6A_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8969313.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14144..15232)
                     /locus_tag="ACHA6A_001098"
     CDS             complement(14144..15232)
                     /locus_tag="ACHA6A_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8969314.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15404..15838
                     /locus_tag="ACHA6A_001099"
     CDS             15404..15838
                     /locus_tag="ACHA6A_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8969315.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15916..16449)
                     /locus_tag="ACHA6A_001100"
     CDS             complement(15916..16449)
                     /locus_tag="ACHA6A_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8969316.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16543..17721)
                     /gene="mutY"
                     /locus_tag="ACHA6A_001101"
     CDS             complement(16543..17721)
                     /gene="mutY"
                     /locus_tag="ACHA6A_001101"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8969317.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17714..18511)
                     /gene="recX"
                     /locus_tag="ACHA6A_001102"
     CDS             complement(17714..18511)
                     /gene="recX"
                     /locus_tag="ACHA6A_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8969318.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18618..20000
                     /gene="rlmD"
                     /locus_tag="ACHA6A_001103"
     CDS             18618..20000
                     /gene="rlmD"
                     /locus_tag="ACHA6A_001103"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8969319.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20174..20752)
                     /locus_tag="ACHA6A_001104"
     CDS             complement(20174..20752)
                     /locus_tag="ACHA6A_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8969320.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21074..21544
                     /locus_tag="ACHA6A_001105"
     CDS             21074..21544
                     /locus_tag="ACHA6A_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8969321.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21652..22878)
                     /locus_tag="ACHA6A_001106"
     CDS             complement(21652..22878)
                     /locus_tag="ACHA6A_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8969322.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23632..23832)
                     /locus_tag="ACHA6A_001107"
     CDS             complement(23632..23832)
                     /locus_tag="ACHA6A_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8969323.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23966..24574)
                     /locus_tag="ACHA6A_001108"
     CDS             complement(23966..24574)
                     /locus_tag="ACHA6A_001108"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8969324.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24659..25129)
                     /locus_tag="ACHA6A_001109"
     CDS             complement(24659..25129)
                     /locus_tag="ACHA6A_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8969325.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25177..26091)
                     /locus_tag="ACHA6A_001110"
     CDS             complement(25177..26091)
                     /locus_tag="ACHA6A_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8969326.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26237..27040
                     /locus_tag="ACHA6A_001111"
     CDS             26237..27040
                     /locus_tag="ACHA6A_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8969327.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27218..28165
                     /locus_tag="ACHA6A_001112"
     CDS             27218..28165
                     /locus_tag="ACHA6A_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8969328.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28233..28526)
                     /locus_tag="ACHA6A_001113"
     CDS             complement(28233..28526)
                     /locus_tag="ACHA6A_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8969329.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(28554..29219)
                     /locus_tag="ACHA6A_001114"
     CDS             complement(28554..29219)
                     /locus_tag="ACHA6A_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8969330.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29359..29991)
                     /locus_tag="ACHA6A_001115"
     CDS             complement(29359..29991)
                     /locus_tag="ACHA6A_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8969331.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(29998..31065)
                     /gene="liaS"
                     /locus_tag="ACHA6A_001116"
     CDS             complement(29998..31065)
                     /gene="liaS"
                     /locus_tag="ACHA6A_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8969332.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31062..31793)
                     /gene="liaF"
                     /locus_tag="ACHA6A_001117"
     CDS             complement(31062..31793)
                     /gene="liaF"
                     /locus_tag="ACHA6A_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8969333.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
BASE COUNT         9584 a         6476 c         5315 g        10578 t
ORIGIN      
        1 ttttctgtga acagaactat agaattccct aaaacttgtc cattatttca aaatttgtta
       61 tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct
      121 ccccatgctg ccgggtgtct atcttatgaa agaccgagat ggtaaggtca tctatgttgg
      181 taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata aacagcattc
      241 aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg tagtagacac
      301 ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc ctttttataa
      361 ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata agggatttgt
      421 attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata aaaaaatgcc
      481 gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg aaattagttt
      541 gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac agaaaaaaaa
      601 ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag
      661 acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat
      721 cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt taagaacacc
      781 atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc actacctcgt
      841 ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc ctgacttcta
      901 ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga
      961 tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt ttgaaattga
     1021 actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gcttctttta
     1081 ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt catagatttc
     1141 catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg tttcagttaa
     1201 ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc cttctttttc
     1261 aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca gtcgttttat
     1321 gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt ctgttatatt
     1381 gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt ttttatgatt
     1441 ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg gttccgtcat
     1501 tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta gttgctggca
     1561 atacaagatc cattttttct gcttcttcta atgcaatctt taaatctttg ataaaatgtt
     1621 tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa ttgcttaaag
     1681 accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt
     1741 ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca atcatgatct
     1801 gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta aatgtcgtac
     1861 caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca ccgaccatga
     1921 tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca tctaacgcat
     1981 ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt gtacttgtag
     2041 tcaaatcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac tgtccatagt
     2101 agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta ctttgttctg
     2161 ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga tcagcttttg
     2221 cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag
     2281 cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc tcctaaaaat
     2341 caaaaatcct tgcttctatg ctagtatacg ctagaaacaa ggattttttc ataaaaaatg
     2401 tttgaatttt atcttttaaa tgaatttaat tttattttga ttcgttcaat cgagtcttct
     2461 tcttcttttc ttcgcatctc ttcttcatga taaacaacca tgtcttgata aaattcgatc
     2521 atagcttcgc cgaaatgtgt agatgaaata tcataaagtt tagcttctaa aacttttgga
     2581 tctttaggaa cttcactgga aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt
     2641 tcaaaagttt tccctaatga aggatgatcg aacaatcggt tcaaataagc atttccttcc
     2701 acgacacaag gtgttccggc tgccatcgct tctgtataag taagcccttg tgtttctgaa
     2761 gtagaagcac tgacaaaata atctgccgct ttataataaa gtgctacatc attgttgtcc
     2821 acttctcctg tgaactgcac aaattcttct aactgcagtt ctgagatcaa ttcctctaac
     2881 tcttctacat aaggtccttt tcctacgata actaatctgg cgttaggata agcggagatg
     2941 acctgaggaa acccattaat caaggcttgg atatttttct cataagaaat cctgcttaaa
     3001 gaaagaatca tcaaatggtt ttcctgaaga cctagtgact cccttaattt gctgatatcc
     3061 tgctgagtga tatcagaacg tttgaatttt tcaatgtcga taccagtagg aatcacgcga
     3121 agcggtgcta ttacaccata gctgcgtaat gtatcaataa cacgttcact tggacaaact
     3181 acacctgtcg tatgatttgc aaataatcta gacaagtatt ttacatggga ttgcctgatc
     3241 acttttcctt taccgatata atgaaggtag tcttcataca tcgtatggta agtatggatc
     3301 agaggtattt tcaacttctt agctaccatc ttcccaagta tccctgctcc gaattctgta
     3361 tgggtatgaa taagatctag ttctagttct ttggcaacca aataagcata ccacatgcct
     3421 ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta cacttggcat tcgaatcaca
     3481 tctttttcca acttatccgc attaggatcg gtcgttgtaa aaatatagac ttcatgccca
     3541 tgtctttcta attcttgttt taatgttttg atcgatgtag caactccgct gacttgcgga
     3601 aagtaggtat cggtaaaaaa gccgattttc atgtttttcg cctccaaact agtccttgac
     3661 ctgatttact ttttttaatt tgctagagac tttttcattc agtacacttt cgtatacatt
     3721 tttaagttct tcaccgattt tattgatact tcgctctttt gctgtttgat aaccggcttg
     3781 tgtcgtactc ggtagttctt gattggtaat ctgttggatc agtcggataa attcttcgtt
     3841 tgtcttcccc atatagcagt tcttgctatc ttctaaccag ccattataca ctggaatatc
     3901 tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa acaacgatcc cttctgtctc
     3961 ttcataagaa gggaaaaaga atagatctgc acctgaatac gcaccttcta tgatctctcc
     4021 acgaatgtaa ccaggaaaca ggacattttc aggatgatct tcttttacaa ttttccggat
     4081 gttccgagga atcgagtaca tgggaacatg accaaaccaa ataaatgtgt attcgggcat
     4141 cttcttagca atttctacga aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca
     4201 aatgattact ttttgatccg gagacagttt gaaatattct ctgaactttt gttctttcat
     4261 tggatcaggg tgatatttct ctaagtcgat cccattggaa atagcttgaa tcggcacacg
     4321 gataccatag ctttccaaca actgttttga ataaggtgtt ggcgtgatca gatagtccgc
     4381 tttttggtac aatcctacca gatatttttt tacgatagga gaaagctggt tcgatctgat
     4441 aaaggaattt cggaaatctt cctctgtaga atgcgcatga tagacaactt tttttccttc
     4501 tcttctggct ttattgacca tattatgact attgattccg tatgtattga tgtgcaggat
     4561 gtcataatct tctttttcat ctagtgtata agagataccg acttccgtca gagcacgttt
     4621 ttgatgatca agcgcacgcc cgattccaga ttttgcaagc atcttttcac tttcaaaata
     4681 taataatacc ttcaccttac gtcccccttc acttatatag tataacatag cctacaaact
     4741 gtcactcaaa cttaagccct atttttgtct attcttactg acaaaaacgt tttcttaaaa
     4801 aagaacaaaa aaactggcgt agctctttta caagccacac cagcttttga tacgttgttt
     4861 attttacata ttcatgaaca agttcaacga cttcttccat catatcgcat tctttcaatg
     4921 cacgatcagc aagttcagcc attttcgctg tgtccaagcg tttcattaaa ctacgcgttt
     4981 taagaataga tgtcgcactc attgagaact catctagacc catacctact agcaatggaa
     5041 cagctgtttg atctccagcc atttctccac acatacctgc ccattttcct tcagcgtgtg
     5101 ctgcatcaat tacatttttg atcaaacgta aaattgatgg gttatatggt tggtacaagt
     5161 aagaaacgcg ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac
     5221 ctacagagaa gaaatctact tcttttgcaa acttgtctgc taagactgct gctgctggaa
     5281 tctcgatcat gatgccgact tggatcgtat cagatacttc cttgccttca cttatcaatt
     5341 tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt gtagctacca
     5401 tagggaacat gatacgtaaa ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg
     5461 tacggaacat ttcgtcgcct tgttcagata aactgatacg caatgcacga tatcctaaga
     5521 atggattcat ttcatgaggt aattgcagat atggtagttc tttatctcca ccgatgtcca
     5581 ttgtacgaac aactacaggt tttccttcca taccttctaa tacagctttg taagcttcat
     5641 attggtcatc ttctgttggg aagtctggag aatccatata caggaattca gtacggtaca
     5701 gaccaactgc ttctccacca ttattatgga caccaactag atcttttggt gttccgatgt
     5761 ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt tttaattttt
     5821 cccattctgc tttttgtgct gcgtaatctg cacctgcttt ttcaaattca gctttttgtt
     5881 catctgtagg atcgatgatt acatcgcctt cgatcccgtt gacagccaaa atatcgcctt
     5941 ctttgacttt agcagtaatt tcttttgtcc caacgattgc tggaatttct aaagaacgtg
     6001 ccatgatagc agaatgtgaa gtacgtccac caatgtctgt tacaaacgct tttacaaaat
     6061 tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg accacttctt
     6121 cgttgatcat tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag
     6181 caacgtcacg aatatctgca gcacgctctt gcatataagc attatcttcc attgcttcga
     6241 acataccgat atacatatcc gttacttctt ttagcgctga ttcagcattt acgctgttgt
     6301 ctttaatatt ttgcttgatt tgacctacca tttctggatc tgaaagaacc atcaaatggg
     6361 catcaaatac ttgcgcttct gcttcaccta agctttgcgc tgctttttca cgaatttgct
     6421 gcaattcttc agtagattta gctaatgcag catccaaacg agcttcttca gccgcagtat
     6481 cttctactgt tgccttactg aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa
     6541 cggctacacc gtcactagcg gcaatccctt ttagcatttc aaccattatt cagataagcc
     6601 ttctttcttc attgtttcaa cgattgctgc cattgcatca gcttcgtcag caccttctgc
     6661 agtaatagta acatcagaac cttgacctac acctaaagac ataacgccca tgattgattt
     6721 caagtttact gatttacctt tatactctaa gtttacatca gagttaaatt tgcttgctgt
     6781 ttgtactaat agtgtagctg gacgagcgtg gatcccagtt tctgctacta cgtgaaattc
     6841 tttcttttcc atattaatcg gtctccttta agtgagtgtt gtttttatgt gagggttttc
     6901 ttttttagta attcagagaa tcctaacaaa tgccaccctt tcatctgtaa agattaacat
     6961 tttttttcgt tagatacaac agttttcatt caatttcaga taaaagtatt caaattattc
     7021 cggattcttt ttcatccttt tcttcacatt ttataacaaa cgtgtttatt tcctttttct
     7081 tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata
     7141 tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa
     7201 tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt
     7261 cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg
     7321 tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc
     7381 cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa
     7441 aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag
     7501 tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca
     7561 aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg
     7621 caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg
     7681 tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa
     7741 tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt
     7801 tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa
     7861 agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt
     7921 tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt
     7981 catagatgaa gttcacgaaa tcgtcggtgc tggttctgct ggtgatggaa atatggatgc
     8041 tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac
     8101 cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt
     8161 acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg
     8221 ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt
     8281 atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga
     8341 aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa
     8401 actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc
     8461 agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga
     8521 agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg
     8581 tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga
     8641 cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg
     8701 ccgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg
     8761 accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc
     8821 acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa
     8881 attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa
     8941 agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga
     9001 tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag
     9061 aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt
     9121 agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca
     9181 attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag
     9241 tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca
     9301 aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt
     9361 tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca
     9421 aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc
     9481 aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga
     9541 atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga
     9601 gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga
     9661 aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt
     9721 ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc
     9781 catctagtca aggtctacac agccggaaat accactattg tctacactga agcccctgaa
     9841 cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga aatcgaagat
     9901 gctaaaaatt atttcttaga taaaatcaaa gacgccgttt accacgagga tgaaatcaaa
     9961 gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa aacatgaatt aggcgctaca
    10021 ttagccgtgt aaacaaattt tgaacataat aattaaagag cgtgggacaa aagtaaagaa
    10081 cacttttgtc tcacgctcta aacacgtata aacggcgaaa gcagaagcaa ctccttcgga
    10141 aataagctga aattcacgaa aattgctttt caaatttttg tgaattccag cttatttctt
    10201 ggagttaaac gcttctgtct cagcctcttt ttctttctag ctttttgatt tttctctttt
    10261 cttttgtact tttctctttt cattttcagt tctacgcgtg tctttatttt taactttttc
    10321 ctctttatgg atgattgatc gtactttttg tgcaatttgc tcgtttttgt cccagtcttt
    10381 tacatctaat aatcgacata atcgtaacat ctcttctttt ttctgttttt ttctttcacg
    10441 tctttgccat tctgtttcca tgtgtcacac tctcctttct ttaatcatat gtacgcaaaa
    10501 taatttttat atttttttga ttagacattt ctatgatttt aatatagtaa aaagagccgt
    10561 cacggaaaat ccgtcacggc tctaaacaaa atatgagagg ctgtttttgt ttggctattc
    10621 ttattacttg atgcagggca gcttgtgtca agacttcttt acatcaaact ttttaatttt
    10681 acattgggat atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa taagaacaac
    10741 ggttggtcga aacggtcacg agccaagata ttgcgacttg aactcatctt ctcatccaaa
    10801 tcttctggat cgatccagcg agcgattttg tgtcccattg gagacatgat tacttcggcg
    10861 ttatactcat ttaacatacg atattggaat acttcgaatt gtagctgacc aacagctcca
    10921 atgatatatt cttccgttag atatgtttta taaagctgaa tcgctccttc ttgaactaat
    10981 tgatagattc ctttgtggaa ggatttttgt ttcatgacat tttttgcagt cactttcata
    11041 aacagctctg gagtaaatga agggagttct tcataagcaa cttttagttt tccttcgaac
    11101 aatgtatccc caatctgata attgcctgta tcatatacac cgatgatatc gcctgctact
    11161 gctttttcga cgttttctct cgaatctgcc ataaattgtg tgacattgct taatttcagt
    11221 tttttatttg tacgttccaa ccagacatcc attcctcgtt cgaatgttcc agaacagatt
    11281 cggacaaaag cgattcggtc acgatgggct ggattcatat ttgcttggat cttgaatacg
    11341 aaacctgaaa attcttcctc ataaggactt accatctgat catcttgtgt tttgtgtcca
    11401 taaggagctg gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc
    11461 aatgcagaac caaaaaatac tggtgtctga tcgccacgag caattttttc ttgattaaat
    11521 gcatctccag cctctttgat tagttctact tcttctaaga cttgttcata taaggaattt
    11581 tcagttagag gatgattttc gggaagcagt ccatcttcat ccaattctac taatcgttca
    11641 tcctgataat tttccggacg ataaaactct acacgattat ggtagatatc ataaagacct
    11701 tctagccctt tacccatacc gattggccaa ttcatcggat aagattcgat atctaatagc
    11761 tcttctaatt cttctaacaa ttccaatggt tcacgaccat cacgatctag tttgttgata
    11821 aaagtaaata tcgggatgcc acgttttttt acaacttgga ataatttctt tgtctgtgct
    11881 tcgatccctt ttgcactatc gattaccatg accgcactat ccacggccat caaagtacga
    11941 taagtatctt cagaaaaatc ttcatgtcct ggtgtatcca agatatttac tcgtttccct
    12001 tgataatcaa actgcataac agaactagta acagaaatcc cacgttgttt ctcgatttcc
    12061 atccagtctg atttggcaaa attacctgtt tttttgcctt ttacggtccc ggcttgacgg
    12121 atagccccac caaacaatag taattgttct gtaatcgttg ttttcccagc atccggatgg
    12181 gaaatgatcg caaaagttct gcgattgtcc acttgttgct ttaattttgg attattcata
    12241 acctaactcc atttcttcca ttcaattatt tatattttct ctaggcacag attgtccgat
    12301 ctcttaaaga aagacaattt catatagggc tgtgacaaaa gccgtgtagc agtctctttt
    12361 tccgagtaaa cggcgttcta gagacagcct atcagcaata agcccaccca agccaaaaat
    12421 acgaaaagct gtttttgctt ggctgctctt attacttaat gcaggatagc tttttcacaa
    12481 cctttgtcaa ctactacaac tgaatcagta tagcatggat tcagcgaatg aaaaagccac
    12541 caaaccataa gagtcaaata tttttttgtc agctgactaa aagaaaagac gtgttgcttg
    12601 tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc
    12661 ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg
    12721 atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag
    12781 cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc
    12841 tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc
    12901 ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt
    12961 ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa
    13021 aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat
    13081 ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac
    13141 gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaagccga atttatgggc
    13201 agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata
    13261 aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc
    13321 gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt
    13381 atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt
    13441 ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat
    13501 cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc
    13561 tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt
    13621 taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc
    13681 cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa
    13741 ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc
    13801 tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga
    13861 tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc
    13921 aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg
    13981 cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc cttataaaat
    14041 taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac
    14101 aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa
    14161 ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga
    14221 tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat
    14281 ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa
    14341 ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca accacacaac
    14401 aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta
    14461 gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag
    14521 cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt
    14581 cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca
    14641 caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc
    14701 cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag
    14761 cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga
    14821 ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca
    14881 aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat
    14941 tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa
    15001 taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg
    15061 ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta
    15121 caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg
    15181 atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag
    15241 actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata
    15301 ttcgttaaaa attcttttat tcttttttta tttttgaaaa cttgccatac tttttatttg
    15361 ttttattttc cagtatacta tacgaaagga aggtggctag accatgaaag ttgtacaaac
    15421 aaaagataca atgagcaata tttacttaga tgctgtacgg atcaggcatc aagtttttgt
    15481 agtcgaacag ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc attgtatcca
    15541 cttcgtcctg tactcagata aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga
    15601 aaacggaaaa atgaaattac agcggatggc gattctttct gaatacagac accaagggtt
    15661 aggaaaaata ttgattgaag aagcagaaaa ctttgctaag aatcaaggct ataatacgat
    15721 tttattaggg gcacagtcca cagctgaaac tttttacgaa aaactaggat atacagctta
    15781 cggcgatcct tttgaagatg ctggcatgcc tcatatttat atgaaaaaga ctttataaaa
    15841 atttatttcg aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc actggcttac
    15901 tgttcttttt tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat
    15961 atagccttcc gagaacgggc catctccatt attgatccag tcaaccagaa tcttcacatt
    16021 ttcttttagg atgaagtcaa tatcgttcgg gtaattcatc attttgctgt gcatctcata
    16081 ttcatcgaca tccaatagtc gtttctcgcc gtcaggaaat accttgatat ccaagtcata
    16141 gtcgatatat ttcaaggcct catcgtccag caaaaaaggt gaagctagat tacaataata
    16201 ggaaaccccc ttttctctga tcattgctat tacattgaac cagtattttt tatgaaaata
    16261 aacaatcgct ggttcgcgcg ttacccatcg gcgtccatca gattcagtga ctaatgtgtg
    16321 atcattcacg ccgatcatag agtgttcgct tgtttttaat accatggtat ctcgccacgt
    16381 tcgatgcaat ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc cttcttttgg
    16441 aactcccata gcaacccttc cttaagcagc attttgtgct ttgtgtttgg gtgttcatta
    16501 caccgacatc ataattatta tatcaataaa aatgagcttt gcctagaaag gattcctatt
    16561 gtttcgattt ttctctaact gatctaccag tttcatttgg accttaggaa aaacgaccga
    16621 atcaaatgca tcaggcttca accacttcga agttttgttc tcggtaaatt cttgttctgc
    16681 tccctcggtt gcccgtccgt aaaaaaggag aacatgccat ttcagatgac taaaaatatg
    16741 agtaacttct cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt caaatgggag
    16801 attttgctct tcttctgcta ctaggtcatc aaacagatcg atttctggct ttgtttccca
    16861 ttcttttttc aatcgctcat actcctcttg tgtcacttcc atcattggaa atgtccacat
    16921 attcgccagc aatttttggc tatctctttc ttcaaaataa tacgctccag aatgattttg
    16981 aagagctgca ctgatataat aaacgtcttt tggctttgct ttttttgttt ttacaggaaa
    17041 actcgtttgg attcctcgtt tgttagctaa acaaaaggct tggatcggac aagtctcaca
    17101 ctttggagaa gttggtgtac agatagctga acctagatcc atcattgctt ggttgaattc
    17161 acctggatgc ttttcatcaa tgattttccg catcgcttca tcaaagattt tcctgctaga
    17221 agcttttgcg atgtctgctt caatgcaaaa caatctgctg actactcgca tcacgttgcc
    17281 gtcaactgca ggttctggaa gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg
    17341 tcctattccc ttcaatgagc taatttcttc aggcgtttgc ggcatttccc catcaaattc
    17401 tgacatgatc tgtttagcag cagcttggat gttccgggct cttgagtaat aaccaaggcc
    17461 ctcccatgct tttaaaagct tttcttctgg agcatttgct agctcttcaa ttgtaggaaa
    17521 ccattccatg aaacgataaa aataatcgat cactgtgtct actctggttt gctgaagcat
    17581 gatctcagag atccagatac gataaggatc acgattatag cgccaaggca ggtttctttt
    17641 ttcttgttca taccattgaa tgaactgatc ttgaaattct tttgtttctt tatctgtcca
    17701 tttttcccat tctttattca ctttcctctt ccttttccgt aatgaatcgc tggatcatat
    17761 ccaaatcaaa tcctttttga tataagcttt gctttacttt tagatttctt tttttaggtt
    17821 caaaccttaa atttcttcgc catagtttat ctccttgttt gactaaataa tcatattcta
    17881 tttcttgatc tgcttcttgc ggaagatcag ctatcgcttg ttggatcact tcttgagaaa
    17941 agcctttcgt catcaatgtt tgttggattt ttctcaataa ttctttttgg ctttttccgt
    18001 gattttttcc aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg
    18061 tatattggct gagcgcttgt tcaatgattt caggtttcaa acctttttgc tgcatctgct
    18121 gcgctaattt ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca taactttccc
    18181 catagaccaa atcatcgaca aggtccaatt cttttaagcg ggctacgatt ttatttcgat
    18241 cttcttgaag gatttccttt tcttttaagt atgttcggat ttctttttcc gatcgcaact
    18301 gataacttaa gtaattcatt gctaggtgaa aaccgaaatc agctccgcta ctcttcttga
    18361 tctcttggat agtttcttcg tctaattccc gtccttttaa taatcgatag cgtactaata
    18421 catcttcaga tacttgtaaa gcttctcctt cagataaatc tacgcgatag aaagggcctt
    18481 tgcctttact gattctcgta acggttaaca taaacatcct cctcgaacat atattctcta
    18541 tcataccaca gaaaatcccc gtaatcaggt attatatgtt agaataaacc acagaaaaaa
    18601 gaaaaaggga gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga
    18661 aaatcaagcg tttaggcata aatggtgaag gaatcggtta tttcaaacgg ttgatcatat
    18721 ttgttccata tgcactacca aaagaagaag tactagtgaa aatcacgaaa gcaactcctc
    18781 gctatgcaga aggagaatta ataaaagtca aaaaaacaag caaagatcgc gtagtggcac
    18841 catgtcctgt ttactatgaa tgcggtggct gtcaattaca acacttagct tatcaagctc
    18901 aattggattt taaaaaggat ctgcttttgc aagcattgga aaaattcaaa ccagcaggct
    18961 ttcgatctta tcaacttttg ccgacgatcg ggatggatga gccttggcat tatagaaaca
    19021 aagcacaatt tcagttacga aaaaataagc agactcaaaa aattgaagct ggtttatacg
    19081 aagcaaactc tcatcagtta gtccctataa cagactgctt ggttcaagaa cctcgaacac
    19141 aaaaagtaat gaatactgtt gttcaactat tgaataaatt tgatgcaccc atctatgatg
    19201 aacggacaaa cagtggaatc ttccgcacaa tcatggttag aatcggtgtt aaaactgggg
    19261 agttacaagt tgtttttatt actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc
    19321 gtgagatcaa tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa
    19381 aaacgtctct cgtgatgggg gatgaaacga tccacttatg gggaaaagaa gccattgaag
    19441 aacaaatcaa tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc
    19501 agcagaccga attgctttat ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa
    19561 cgatcgtgga tgcttattgc ggagttggga cgatcggctt aagtgtagca aaacaagcca
    19621 aagaagtacg gggaatggat accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg
    19681 aaagattagg atatcacaac actcgatatg aagtcgggac agcagagcaa gttctgccta
    19741 aatggttgaa tgaaggtttt cagcctgatg gtatcatcgt agatccacca cgtacgggtt
    19801 tggataacgc attgatccaa tcattattga aataccctcc aaaaaaactt gtttatatct
    19861 cctgtaatgt ttctacactt gccagagatc tggttaattt aagtaaaaag ttcaagattg
    19921 aatatttaca gtccgtcgac atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga
    19981 tgacgagact cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa
    20041 taagaacagc caaacaaaaa tagctcttca tgttttatga atactgtact tgtgaccgaa
    20101 gagttaacag gaggacacta tttatttaaa aaaagggttg tgacatagtt tttgtcacag
    20161 ccctttttga ttattattgg atattcacgt ctatcacttg ataaaatgca tttgtcgtat
    20221 cgctgatatt ccatactgct aaaatcacat tatatccttt tcgatcctgc ggaagtgtga
    20281 tcgtttggtt gactgttttt ggtggaacag ttgctttgtc gtctattttt gtgatcaatt
    20341 caaaatcact aaatttcaaa ggttgattag gattccaagt tggtttggta atgtagtaat
    20401 cccaagtact tgtccgatgt tgtgctgtta aattccaagt aatatttaat gttcctggat
    20461 taaccatgtt tttatgccat ctgttagatg tctgttcatc taaaggctca aagccgctga
    20521 ctcctgcact agcaattttt ccatcaatga atgtattttt ggtagcttcg atactttgcg
    20581 gttcccattg tgctcttccg acattttgat ttaagtttcc tgctgctgat gttcctaggt
    20641 atgcacggct tcctggagaa gtaatataac catgcgcaaa acctggaatg ctaaaaccga
    20701 acaaccctaa actaactaac ccaataaaca ataacgtact aatctttttc atgtagaaac
    20761 ctccttcatt tgataaccac attgtaaacg ctttattgta ttaacacaaa cattatgctt
    20821 ttataatttt atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag
    20881 tagacgagaa aagtacaaat agaaaacatg agaatcaaat catatgatct ttttcaaaaa
    20941 catcattcat ataaacaaat gttcaacttt ttaataacaa caattatcaa tttttttctt
    21001 acttcacaaa ctaaatacag gtcttgctgt gagccttcat ggtaaactaa tattatagaa
    21061 aggagttagt aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg ttataggaat
    21121 tgtcatatta ggctttttat cagggttgct tagcggaaat cccggaaaat attactattc
    21181 tttgcaactt ccaccatttg caccacctag ctggatattt gggccgatgt ggaccttact
    21241 gtatatattg atgggtattt ctctttattt gttacttaat cacaacaaca aaaaacagcg
    21301 aaacaatctt gtcggtcttt ttgtgattca gttcattttt aattttatat ggtctgctct
    21361 attttttaat ttaagaaata ttttcattgc agcaatcgat attacacttt tagttatttt
    21421 cttgtcagtg cttatgtatc agttgtggct gcatcatcgc ctggcaatgt ggctcatgat
    21481 tccttattat ttatgggtac tattcgcaac tctattgaat tattcaattt attttttaaa
    21541 ctaagcgcta aaaagagacg tcctgtaatt taacacatag aaaaagtgcc acctaagtta
    21601 gattttttgt ctaatttagg tggcactctt tattaaccaa actatttttt tctagtaccc
    21661 acttgttgtt ccatactcat tattctggtc ataaccataa ccattttgtt cctgtccagc
    21721 gtaaggatca taactttgag tatcttgact tggtgtatag tactgacttg tatcttctgt
    21781 ttgctgataa taggtattcg gatcttggta ctgatcatat gtgttttctg aataggaaga
    21841 ataatcggtg tacccttcac tattcgatcc atcaccgttt gtcgaatcgc catcttccga
    21901 atcatcaaag aacatcattg aagcattctc atttttcaaa ttaggtaatg tatcgcttgt
    21961 ttttatattt aattgtttct tcagttcatt ttggatcgaa agaagatcat tttttcctaa
    22021 aatttgatag tagatatcat tgatcatttg tccttcgcct tgcaactggt cttgtttgat
    22081 tgtttcaaac gccggggtgt agttcgtcgc tacatcaagc atgtcatccc aatctaagtc
    22141 cgtcgtcaca ttgttctcaa ctgcttttaa aatttttcta taattgctta cgccatctaa
    22201 acttaaaact ttattgacga ttttcgtcac tacttcccgc tgacgacgtt gtcgaccgat
    22261 atcgccttct ggatcttcgt gtcgcattcg agaataggct aaaccttttt ctccattcaa
    22321 tgtttgttca cctactggta catggacacc atccaaagta aagtcaattt tattattcac
    22381 tttgatgcca ccgaccgcat tgatcaaatc ttccatgcca tccaaattga tcgttacata
    22441 atgatcaata ggaatatcga gcagttgctc aattgaatcc atggccattt cgacaccacc
    22501 aaatgcgtag gcatgattta atttatctac cgttccataa cctacgatat tcgtataaat
    22561 atctcggtcc aaactgatga tcgttgattt tttttgtttc ggattaactg ttactaccat
    22621 catcgtatca gaacgtcctt gttccgttct tcctaatcca ccggtatcta aacctaatag
    22681 caaaaccgaa aaaggttctt tgtcatcaat gcttactctc tcagaacgtt tggaggatat
    22741 tcgattagag tttttagaaa ttttgtttac tgtttggctc gcttctccca taaatctgat
    22801 tccgtatgct gataatccgg caatcagcaa aacgaatacc cctaacaagg ttaatagaac
    22861 tttttgccat cgcttcataa tacacctctg tttcacattt tctaaaatat tgtctcatat
    22921 aactgttcaa tcagcaaatt cactttttaa tcataagaaa actttaaaaa acatttttgt
    22981 gtaacgatta ttatttttta aaatttctca aaattcttaa ctatcatacc ataccgtgaa
    23041 aaagatcgaa agaccgaata cacgcagttc gcaatcgttt tatctttttt tatgtatttt
    23101 ttccagaaaa agcgaacaac aaggtatttt ccaaataatc gtgcagtaaa atgatcgcac
    23161 atacctttcc catcttttta acagtctttg cctaccgttc agattatcat tcgatttcac
    23221 gacaatttct tcctatgact ggtccttttg ttaaacccat cttctattcg ttttatttcc
    23281 agctcactgc atggctctta aaatgactcg aactagtgat tgcattacca tcatctttta
    23341 atggatagat atgctcctct tcaaagtaaa acgattttct tttaaagtaa tagaattgac
    23401 atggtaatga gtatgtatac tagtggtttc acaaatggtc ttttattcaa catccaaaaa
    23461 ctgaaatttc tttaaaatcc attactaacg aagaaaatgt tccttgcttg ataattagtt
    23521 aggacatgtc aagtgaacaa atgcatttac ttagccaaaa aatctaaaaa cttcataaat
    23581 agacaaaaag acacgactct ctaagaagtc gtgtctttat tcaacaaatt attataattt
    23641 agtcacttca gcagcttgag gtccgcgtgc accttcaaca atcgtgaatt ctacttcttg
    23701 accttcttct aggctcttga agccgtctcc ttgaattgct gagaaatgaa caaatacgtc
    23761 atctccgccg tctactgtaa taaacccaaa tcctttttca ttgttgaacc atttaaccgt
    23821 tccgtgttcc ataaatgtat atcctccaaa aaagttcttg caagtaacct tgcaagaaca
    23881 ttatacccaa tttagaaaac gctagcaagc tttcgctctt ttattttcca aattattttt
    23941 aagactgata tgacggagga aatggttatt ttgctgcttc gaaacgtttg tttacttcat
    24001 cccaattaat aacattccag aatgctgcaa tataatctgg tcggacattt ttatatttca
    24061 aataataagc atgttcccaa acgtccaaac ctagaattgg tgtctttcct tccatcaatg
    24121 gagaatcttg atttgcagta gaggtaatag ctaattttcc attttccatt acaagccaag
    24181 cccatccaga accaaatcgt ccagcggctg cttttttgaa ttcttcttta aaagaagaaa
    24241 aatcaccaaa agcttcatta atcgcttctt ttatttctcc tgtaggttcg ccaccagcat
    24301 ttggtgccat gatttcccag aaaaatgaat ggttagcatg tccgccacca ttgttacgta
    24361 cagctgtctt gatatctgtt ggaatagcgt ccatatcaga taataattct tctattgttt
    24421 tttcgcctaa ttctgggtat ttctcaattg ctgaatttaa attcgtcaca taagtattgt
    24481 gatgcttatc atgatgcaga tgcatcgttt cttcgtcaat ataaggttcc aatgcgtcat
    24541 atgcataagg tagatctggt aatgtgtaag tcataaaaaa attcctcctc aatgttttct
    24601 taataatgtc tacattacaa agagtaacaa agagaaggaa attcttcaat tgttatgcct
    24661 agtcgtcttt ttcactttga cctacttctt cttcaaaaac ctcagtttct ttaaaaataa
    24721 acaacttgct gaaaatatag ttcgcaagga taacaataat ttgagtgatg attttcgcca
    24781 catactcatt gctgttcata acattgatca acaataccat tgcccccatg tccaatccgt
    24841 aagaaaggat acggtagaag aagaacttac cgcattcaat aagtgcatgt gtaaaattcg
    24901 gtgattttga atggaatacc caaagtttgt tcgttacaaa agcaaacaat acagaacaaa
    24961 accacgaaat agaattggat aaggccatac ttaaatcaaa atattggcgt gcgagagcaa
    25021 aactaacaaa atttacaatc gtagccaaac caccaaaaaa gagatagact agtatctccc
    25081 aatatccttt tgcttctaaa tatcctttta actgtcgata ctgcttcatg agccaaccta
    25141 ccttattaaa aattcttatt tatcatactc tatatttcaa taactttcat ctttatttaa
    25201 aaaaattaat tttttttgat cctttgcctt tgtctctgct tggatcgtca catgtgtaat
    25261 accatatttt aattttaata aagaaattat acgaggatat atcttttcaa tctcgcttag
    25321 tagctgatct tcaaaatcaa tatgtccgtc gaaaataatt atcttttcgc tatactgcca
    25381 aatatggaca tgatgaacat cctttacccc atcgattgct tcaatttctt gttccatttt
    25441 ttcatagtcg atagcaggtg cactttgtat caaaatagcg gcagcttttt tgatcaccat
    25501 tatcgcttct atcaatatat atatcgaaat gatcagtgta ataactggat cgatccagta
    25561 gatatcgaaa aaacggatca aaattgctcc gataatcaca cttaccgaag acagtgtatc
    25621 acttaacaaa tgcaaataac tagattttat attcaaactt tcatctgctt cttgtcgtaa
    25681 taataatact gagaacaaat tcgcgagcaa tccaataact gctactgtta gcatcaaatg
    25741 actatttatc ttttctggtg aagtgaaccg cttgaatgct tcaacggtta aaaacaagga
    25801 taatatgatc aatatgaatg agttgatgaa agcactcaat atttctaaac gctggtagcc
    25861 atacgtacgt tttttatttg cttcttttcg ggccattttt tgtgccccgt aagaaaaaat
    25921 aattgccaat gtatcactaa ggttatggaa agcatctgaa acaagagaca aactccctga
    25981 cataatccct ccaataaatt ctgataaagt gatcaccgca ttcaaaacca ctactccaaa
    26041 tagttttttt gatgatctct gtggctgtgt aggcactatt ccttcctcca ttttatttat
    26101 attggatagt ctttagacaa gaataacatt tttttgatat ttcaccaatc attcagctgt
    26161 ttccttaaag cacttgtatt tcacttgata cttcgctaga attgcaagtg atgacactac
    26221 tagatgagga gtacttatga caacaaaaca attaattatc agttccttta tacgattcaa
    26281 agacctaaag gaggcgagaa atgtcccatt ttggaaagcc attgtctatc ttttagtttt
    26341 gagtataatt atggctttgc caatctcttt tcaagttttt caagtcttaa acaacattca
    26401 attggatggg caaaagattg ctactcgcat tcctgatttt acaatccaaa acgggcagct
    26461 tcaaaccgaa gaaaaagaag gattcattta ccaaacaaac tccattatct tcacatttga
    26521 tccagaggga aaacgaacag aacaggacat ctctactgat ttgatgggga attttgttag
    26581 tgtcgggatg ttaaaaaaca aacttatcat agctcttcct aatacaggca cgacttctgc
    26641 tttgttgaac aataaccaat tagaacttcc ctacaccaat gaatctttaa aaaacttaac
    26701 aggtaagcag ctccgttctt ttttaagtga agcatctata ccactttgga taaaagtcct
    26761 gacatttata ttttctattt atcctagttt tctgaatctg ctgatcactt tgctatttac
    26821 caacattgct gcttttctct atgcacgctt tcgactaaca aaagctactt ttcttgattg
    26881 tttaaaaaca ttgatctatt caatttcatt gcctactata attgcaacaa tcttgatggt
    26941 ctttcttcct tcttttgaca ctagcgcctt cattgctttt gcaggaatct ttatttttgc
    27001 tcaagctgtc aaaggatggg caaaaatatc gatcaattaa gaaataaaga caactcgtta
    27061 tttctacttc aatagaccct tttagaggtt gtgacatttt tttgtcacag cccctttttt
    27121 atttgaaaaa tatttctttc tcaaaaaaca agcaaggcgc ttcaaagttc ttcttgtatg
    27181 cgctacactt agagtaaaga aaggcgggaa attaactatg tcagaaagaa tcatttatgc
    27241 agatcgtcca tttcgtgatg aatttatcga agaaatccat actatagcgc ctggttatat
    27301 ctttaaaaca gaattacaac cagaggatct acaaaatgta gagatcagtt tgggctggaa
    27361 ctcagactac caagaaaaac tattagcctc ttctaccttg aaatgggtcc aatctatctc
    27421 agctggtgtt gatcagctac cccttgagac tttttcaaag aaacatattt tactttcaaa
    27481 tggtagtggc atccacagtg aatcgattac ggagcatgtc ttaggaatca tccttgggta
    27541 ttctcgtggt ctttttcaag cacaaaaggc acagagtgac atgaaatggt taggttcatc
    27601 ggtccattac caatctatcc aagggaaaaa aatactaatc attggaactg gccatattgg
    27661 aaaaatgatt gcaaaaaaat gcaaagcatt agacatgaac tgctatggaa tcaatacatc
    27721 cggtcatcca gcagaaggaa tggaaaagac tttttcttta aatcaactaa aggaagttct
    27781 tcctgaagta gatttcgtag tcaatatctt acctctaacg gaggaaacaa ctgggctctt
    27841 tgatcagcat atgtttgaac actttgatcc aaaatctgta ttcatcaatg ttggccgtgg
    27901 tgcttctgtt aagacacaag atctggtgca ggctttaaat aaccagcaac tttcctttgc
    27961 agcattagac gtttttgagg aagagcctct tccacaagat catcctcttt ggaaaatgga
    28021 caatgtactg attacttccc acattgcagg tttgacacct gattttcaaa agaaattaat
    28081 ggctattttt ttaaccaatc tgaaaagcta ctttgccacc catgagcttc aaaccaatca
    28141 agtaaaattg tctgcaggat attaatagct gaaaacaata ttttcaaaag aaaaaagaga
    28201 aggtgacaaa aattgtgtca ccctctcttt ttttattgtt ttttaaagtg ataggttggc
    28261 tcctccaggt tgggattata atcaacaagt aaatcatatc ctttaaagaa ccattcatct
    28321 gcttcctcga taaaaaaaag tgtcccatca atttccactt tctttacagg atgttctggc
    28381 tgttcaactg acattccaat cgaaaaacca tcatgaacct ctgttttgcc gtagactttt
    28441 ccaaaaaaac gtattccgta agtttctggc agatcaattt ctgagtcaaa ccacttttgt
    28501 gcttttggcg ttaccgttaa tttcatccca acaacctact ttctcttttc tttctattga
    28561 ttcatcttat cgtctagaat atccacatcg cctgtctctc caataaccag tagattatca
    28621 ttttcacgaa catattcatc tgctgcagga gaagcgataa ccgtttgatc tactcttcgt
    28681 atggcgacta ctgttaagtc aaaacgttgc ctgaaattta attcagctaa tgttttgttg
    28741 aaaaatttag gattggagac tcggatttct gctaaagaat atttatctga caactctagg
    28801 taatctaaaa tattctttga cactaaatta tgcgcaatcc ttattcccat gtctcgctcg
    28861 ggatggacga cgcgatcagc tccgatcttt tcaagtactc tggcatgata ctcattttgg
    28921 gcttttgcta aaatatttgg tacgcccatt tcctttacca tcagagtaac taagatactg
    28981 gcttgaatat cttctccaat agcgacaatt acatgatcga aatttcttat gcctaatgat
    29041 cgaagtgtca tttcatcctg tgcatttgcc acaacagcat gagtggctat attcatatat
    29101 tcattgaccc gatcttcatt actatcaata gccagtactt cttgaccagc ttcgatcagt
    29161 gttcggcaga tacttccgcc aaatcgtcca agacctacaa ttgcaaaatt ttgtttcacc
    29221 gttttcctcc tttagtatct tcttttctca gtataacaaa gcctttacaa aaacaaaagc
    29281 aagcatcatt attgtaaaaa agccaagaaa aataatagtt attttctcag cttttgatta
    29341 tcctctttat catcgttttt atttcaccaa accatgtttg aatgcataga tagcggcttg
    29401 tgtacggtct tccacttcta gcttagccaa tatattggaa acatgtgttt ttactgtttt
    29461 cagtgtaata aacaattcgt ccgctatttc ctgattactt tttccttcag aaatcagcat
    29521 taaaatttca ttttcccgat tcgttagttc ttcatgtaat acaggctcat ttcgccgact
    29581 cattttttcc atcatttttg ttgtcacttc tggttctaat actcgctcgc cacgttgcgt
    29641 tgcccttata gcatcagcaa tttcatgtgc ggtagaggtc ttcaacaaat accctgctgc
    29701 acctgcttca atggcaggat aaactttttc gtcatcaata aaactggtta cgataatgat
    29761 tttagcttct ggccaatctt ttaagattgc ttttgttgat tcgataccat ccatttcttc
    29821 catgactaag tccattaaaa tgacgtccgg tcgaagtgtc atcgccttct catagccttg
    29881 tcgtccattt tcagcttctc caattacttc aatgtcttct tgaatagata aataaaaaga
    29941 gacgcctaag cgcaccattt catggtcatc tactaataaa acttttatca tccttatcta
    30001 tgcctccttc atcaaaggga ccttgatttc tacacttgta ccttgacctt taaaactaat
    30061 gatttttact gttccaccaa taccagctac tcgttctttg atattgttca atccataact
    30121 tcccgttttc gtttcattca tattaaaccc tgttccgtcg tcaataatac gtaacaaaag
    30181 attattgtct attttgtgca aatatacctc taattcattg gctttagcat gtcttaatgt
    30241 atttgataac aattcttgaa cgattcggaa caagtgatcc tcaatggaac tagatagttt
    30301 tacatcttct acatcccatt tcagcgaaat ctggatttta ttttgcaatt ccttcaataa
    30361 ctgctcgata ccttgcttta aacttttctc ttctaaattg actggacgta gatgaagaag
    30421 cagcgcacgc atctcagact gggaagcgtt gatgatctct gctaccattt tcaactgctt
    30481 gcgaaacatt tctggtgtct cgcttttttc tgcttgctcc gtcaatgccg acatcatcat
    30541 cattgctgca aacaattgtt gcgaaactga atcatgcagc tcacgggcta atcgatgtct
    30601 ttctaattct aaaatttctt ctttggtttg tccatctaca tattggggac ggctcgttac
    30661 aatttgtaat tcactagata cttcaatcat tttttctttt atcttagtga tttccttatc
    30721 aatatctttg atgtataaat cgtccgatgc acttgggatc ggaagaagca acaatttgga
    30781 ttcataattg ccagaagaaa gtgcacgcag cttttcttcg attttcccgt attttgtctt
    30841 ttgaataagt gataataata gaaagacaat taatcctaca cctaaggaaa tggctaacaa
    30901 atggaagata agcggaacat ataataaacg tgcacgcagt aattctcccc accaatgact
    30961 ttgattgctg gcataaaaat aagtgaaaag tgaaaataga ataataagga aagccgcaat
    31021 ccccgaataa acagctagca tcgctctgga tatttttccc atcatattcg tatcacctcg
    31081 acatctccaa tcaaggtatt agtaataatc ttcaatctac gagggttttc atcataatca
    31141 ctgctgtaga ttttgatttg ttcatttttc aaagaaaatt gctcttcttc aaacaagaca
    31201 tttcctacta atgtcgcatg ctctaatcgg atggctacgc ccaaaggtac taagatccgt
    31261 gttctgccta tcccttttct tacaatcaca atattatcat cttttggcaa aagcgtattg
    31321 cccaaatcta tgatagtatc accagaaatg acggcgatgt tgatatcgtc ccattcatag
    31381 acgtcgtttc caatacgttg gttaccgaat aattgctgtc ttttccgctc attattatgt
    31441 gtttttaatt gctctgtttg gatcatcatg atctgtttct ttcgccaaaa agcatttttg
    31501 gttaaatcta tccctgaaat ctctacacct ttcagaccaa taaataaaac ggcaaataca
    31561 gccatcatcc ataaagcagg actgttgact aagctaaaaa aaatgaccaa acttcctaat
    31621 atcaattgaa aattttgaaa tttagtacga ggatgtttgc gcatagctag atatatattg
    31681 aaaataccga atatcactaa aagcaaaagt ccagtattgt ttacgatttg ccaaacagca
    31741 aataacagca gtagtgcttc aactaccaca aaaaaacgcc aagaactatt catcgctctt
    31801 tctccctcct gacacattct catttcatca tacctatttt tttaactgtt ggctatagga
    31861 ctttagatgt aagatcgttt gttgaaatct aaaatacaca aagagaaaaa gatctagagt
    31921 gtatcatcag taagatgaac tctctagatc ttt
//
LOCUS       DAYYHS010000024        30210 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000024 DAYYHS010000000
VERSION     DAYYHS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.024"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHA6A_001118"
     CDS             complement(<1..1031)
                     /locus_tag="ACHA6A_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8969334.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHA6A_001119"
     CDS             complement(1113..1802)
                     /locus_tag="ACHA6A_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8969335.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHA6A_001120"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHA6A_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8969336.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHA6A_001121"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHA6A_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8969337.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHA6A_001122"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHA6A_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8969338.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHA6A_001123"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHA6A_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8969339.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHA6A_001124"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHA6A_001124"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8969340.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHA6A_001125"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHA6A_001125"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8969341.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHA6A_001126"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHA6A_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHA6A_001127"
     CDS             complement(7636..8523)
                     /locus_tag="ACHA6A_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8969342.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHA6A_001128"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHA6A_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8969343.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHA6A_001129"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHA6A_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8969344.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHA6A_001130"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHA6A_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8969345.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHA6A_001131"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHA6A_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8969346.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHA6A_001132"
     CDS             complement(10454..10957)
                     /locus_tag="ACHA6A_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8969347.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHA6A_001133"
     CDS             11554..12519
                     /locus_tag="ACHA6A_001133"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8969348.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHA6A_001134"
     CDS             complement(12618..13790)
                     /locus_tag="ACHA6A_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969349.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHA6A_001135"
     CDS             complement(13777..14922)
                     /locus_tag="ACHA6A_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969350.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHA6A_001136"
     CDS             complement(14936..15868)
                     /locus_tag="ACHA6A_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969351.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHA6A_001137"
     CDS             15985..17064
                     /locus_tag="ACHA6A_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8969352.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHA6A_001138"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHA6A_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHA6A_001139"
     CDS             complement(17789..18016)
                     /locus_tag="ACHA6A_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8969353.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHA6A_001140"
     CDS             complement(18119..20707)
                     /locus_tag="ACHA6A_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8969354.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHA6A_001141"
     CDS             complement(20727..21386)
                     /locus_tag="ACHA6A_001141"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8969355.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHA6A_001142"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHA6A_001142"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8969356.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHA6A_001143"
     CDS             complement(22159..23025)
                     /locus_tag="ACHA6A_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8969357.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHA6A_001144"
     CDS             23134..23775
                     /locus_tag="ACHA6A_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8969358.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHA6A_001145"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHA6A_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8969359.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHA6A_001146"
     CDS             complement(25216..26115)
                     /locus_tag="ACHA6A_001146"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8969360.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHA6A_001147"
     CDS             complement(26117..26914)
                     /locus_tag="ACHA6A_001147"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8969361.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHA6A_001148"
     CDS             complement(26892..27596)
                     /locus_tag="ACHA6A_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8969362.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHA6A_001149"
     CDS             27727..28302
                     /locus_tag="ACHA6A_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8969363.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHA6A_001150"
     CDS             28420..29097
                     /locus_tag="ACHA6A_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8969364.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(29187..>30210)
                     /locus_tag="ACHA6A_001151"
     CDS             complement(29187..>30210)
                     /locus_tag="ACHA6A_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8969365.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHG
                     KTFLTK"
BASE COUNT         8897 a         6373 c         5456 g         9484 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    29101 aggctctttg tcaaatgatg ttggtaaaga gaaatcttag aataatttgg acagaacaga
    29161 aagaagaaaa cctctttctg ttctgtttat ttggttaaaa atgttttgcc atgacgaatc
    29221 ataattcgta gtttgaaatt gtaaaagttg cgaaagccgt acgcattgcg ttttaatact
    29281 ttgatgtggt tgtttaagca ttccaatggt ccgtttgaat agctgtagtg aagcgcattt
    29341 tggatctgtg gcagaaactt tttaaacgtt cgtaaagtgg ttgtataggt ttctggcaga
    29401 ccgctaatgt ctctagttaa acaatcctca aatagtgact gatctctttt ctttaccgca
    29461 taaagaatgt cttggtataa ctgatagcct tggcgtaagt tgggacaagc agctaacagt
    29521 cgatcggcaa tctcactttc agtcaaataa tctttaaaac tggcacgcca aacccgtttt
    29581 tcatagttta actttgcttc ttctttttgt aggagtttcc aatagcgctt caattttttc
    29641 cctaaccctc gttgagagtg ggaatttcct ttaagtcgta ccgtggtttc tttcactcga
    29701 tgattcaaga aagttctgcc aatatgttgg acgatgtgga aacgatcgat gatcaactga
    29761 gcattcggga aaagcttttt caccaacgaa acataaggcg catacatatc aataacaatc
    29821 aattgaacgg cttcacgagt cgctaatgaa aaccgagaaa aatagcgttc taaataaggc
    29881 aagcgtcgat tttcgacaat atccaataat gcatggcttt gtccgtccat catgataaaa
    29941 ctcattgcac tggcaacact tcgaaccgat ttgaattcat caaagcataa gacctttggc
    30001 aaagtctgtt tgaaaggatt caatgggcgg tagaatcgct tcatgacacg atagaccgaa
    30061 gaagtggaaa tatttttctg acgagcgatt tccgtcatgg aagtattctc ttttaagcga
    30121 gcaatgacag accaacgaac tttttgacta atgaaacagt gtttttcagc aacagaggta
    30181 tccgctacga atgttctttg gcaagactta
//
LOCUS       DAYYHS010000025        30108 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000025 DAYYHS010000000
VERSION     DAYYHS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.025"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..2424)
                     /locus_tag="ACHA6A_001152"
     CDS             complement(61..2424)
                     /locus_tag="ACHA6A_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM8969366.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2589..3093
                     /locus_tag="ACHA6A_001153"
                     /pseudo
     CDS             2589..3093
                     /locus_tag="ACHA6A_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHA6A_001154"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHA6A_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8969367.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHA6A_001155"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHA6A_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8969368.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="ACHA6A_001156"
     CDS             complement(5894..6205)
                     /locus_tag="ACHA6A_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8969369.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="ACHA6A_001157"
     CDS             complement(6202..6495)
                     /locus_tag="ACHA6A_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8969370.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHA6A_001158"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHA6A_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8969371.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHA6A_001159"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHA6A_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8969372.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="ACHA6A_001160"
     CDS             complement(8333..12685)
                     /locus_tag="ACHA6A_001160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8969373.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="ACHA6A_001161"
     CDS             complement(12897..14606)
                     /locus_tag="ACHA6A_001161"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8969374.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHA6A_001162"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHA6A_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8969375.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="ACHA6A_001163"
     CDS             complement(16103..16903)
                     /locus_tag="ACHA6A_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8969376.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="ACHA6A_001164"
     CDS             complement(16900..17712)
                     /locus_tag="ACHA6A_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8969377.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHA6A_001165"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHA6A_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8969378.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHA6A_001166"
     CDS             complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHA6A_001166"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8969379.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHA6A_001167"
     CDS             complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHA6A_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8969380.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHA6A_001168"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHA6A_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8969381.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="ACHA6A_001169"
     CDS             21446..21925
                     /locus_tag="ACHA6A_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8969382.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="ACHA6A_001170"
                     /pseudo
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="ACHA6A_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23226..24011
                     /gene="phnX"
                     /locus_tag="ACHA6A_001171"
     CDS             23226..24011
                     /gene="phnX"
                     /locus_tag="ACHA6A_001171"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8969383.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHA6A_001172"
     CDS             complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHA6A_001172"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8969384.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHA6A_001173"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHA6A_001173"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8969385.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHA6A_001174"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHA6A_001174"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8969386.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHA6A_001175"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHA6A_001175"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8969387.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8050 a         6461 c         5347 g        10250 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcata ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattat
//
LOCUS       DAYYHS010000026        29964 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000026 DAYYHS010000000
VERSION     DAYYHS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.026"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..192
                     /locus_tag="ACHA6A_001176"
     CDS             <1..192
                     /locus_tag="ACHA6A_001176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969388.1"
                     /translation="KPFSQNFSYYSKLSDFFTTSSLNGVQKLASAVISPTKRKIGGAI
                     FVWLFLLLDAEQLFHNLFL"
     gene            332..1201
                     /locus_tag="ACHA6A_001177"
     CDS             332..1201
                     /locus_tag="ACHA6A_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8969389.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1410..2435
                     /locus_tag="ACHA6A_001178"
     CDS             1410..2435
                     /locus_tag="ACHA6A_001178"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8969390.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2474..3424
                     /gene="manA"
                     /locus_tag="ACHA6A_001179"
     CDS             2474..3424
                     /gene="manA"
                     /locus_tag="ACHA6A_001179"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8969391.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3518..5221
                     /locus_tag="ACHA6A_001180"
     CDS             3518..5221
                     /locus_tag="ACHA6A_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8969392.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5223..7256
                     /locus_tag="ACHA6A_001181"
     CDS             5223..7256
                     /locus_tag="ACHA6A_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8969393.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7267..7707
                     /locus_tag="ACHA6A_001182"
     CDS             7267..7707
                     /locus_tag="ACHA6A_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8969394.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7907..9034)
                     /locus_tag="ACHA6A_001183"
     CDS             complement(7907..9034)
                     /locus_tag="ACHA6A_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969395.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9037..9714)
                     /locus_tag="ACHA6A_001184"
     CDS             complement(9037..9714)
                     /locus_tag="ACHA6A_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8969396.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9978..11744
                     /locus_tag="ACHA6A_001185"
     CDS             9978..11744
                     /locus_tag="ACHA6A_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8969397.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11759..12196
                     /locus_tag="ACHA6A_001186"
     CDS             11759..12196
                     /locus_tag="ACHA6A_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8969398.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12209..13360
                     /locus_tag="ACHA6A_001187"
     CDS             12209..13360
                     /locus_tag="ACHA6A_001187"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8969399.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13422..14120)
                     /locus_tag="ACHA6A_001188"
     CDS             complement(13422..14120)
                     /locus_tag="ACHA6A_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8969400.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14113..15465)
                     /locus_tag="ACHA6A_001189"
     CDS             complement(14113..15465)
                     /locus_tag="ACHA6A_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8969401.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15688..17088)
                     /locus_tag="ACHA6A_001190"
     CDS             complement(15688..17088)
                     /locus_tag="ACHA6A_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8969402.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17249..17986
                     /locus_tag="ACHA6A_001191"
     CDS             17249..17986
                     /locus_tag="ACHA6A_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8969403.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18111..18590
                     /locus_tag="ACHA6A_001192"
     CDS             18111..18590
                     /locus_tag="ACHA6A_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8969404.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18587..19354
                     /locus_tag="ACHA6A_001193"
     CDS             18587..19354
                     /locus_tag="ACHA6A_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8969405.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19584..20153
                     /gene="lepB"
                     /locus_tag="ACHA6A_001194"
     CDS             19584..20153
                     /gene="lepB"
                     /locus_tag="ACHA6A_001194"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8969406.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20257..20859)
                     /locus_tag="ACHA6A_001195"
     CDS             complement(20257..20859)
                     /locus_tag="ACHA6A_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8969407.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20965..21195
                     /locus_tag="ACHA6A_001196"
     CDS             20965..21195
                     /locus_tag="ACHA6A_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969408.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21186..21434
                     /locus_tag="ACHA6A_001197"
     CDS             21186..21434
                     /locus_tag="ACHA6A_001197"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8969409.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21540..22124
                     /locus_tag="ACHA6A_001198"
     CDS             21540..22124
                     /locus_tag="ACHA6A_001198"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8969410.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22127..23200
                     /gene="prfA"
                     /locus_tag="ACHA6A_001199"
     CDS             22127..23200
                     /gene="prfA"
                     /locus_tag="ACHA6A_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8969411.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23193..24032
                     /gene="prmC"
                     /locus_tag="ACHA6A_001200"
     CDS             23193..24032
                     /gene="prmC"
                     /locus_tag="ACHA6A_001200"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8969412.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24025..24204
                     /locus_tag="ACHA6A_001201"
     CDS             24025..24204
                     /locus_tag="ACHA6A_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969413.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24226..25251
                     /locus_tag="ACHA6A_001202"
     CDS             24226..25251
                     /locus_tag="ACHA6A_001202"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8969414.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25281..25409
                     /locus_tag="ACHA6A_001203"
     CDS             25281..25409
                     /locus_tag="ACHA6A_001203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969415.1"
                     /translation="MIQCYQVNLLSVNEKLSVTNRNRRDTNIWQFVFDGKNKIVGK"
     gene            25442..26686
                     /gene="glyA"
                     /locus_tag="ACHA6A_001204"
     CDS             25442..26686
                     /gene="glyA"
                     /locus_tag="ACHA6A_001204"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8969416.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26839..27786
                     /locus_tag="ACHA6A_001205"
     CDS             26839..27786
                     /locus_tag="ACHA6A_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8969417.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27920..28549
                     /gene="upp"
                     /locus_tag="ACHA6A_001206"
     CDS             27920..28549
                     /gene="upp"
                     /locus_tag="ACHA6A_001206"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8969418.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28688..28930)
                     /locus_tag="ACHA6A_001207"
     CDS             complement(28688..28930)
                     /locus_tag="ACHA6A_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969419.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29051..29123
                     /locus_tag="ACHA6A_001208"
     tRNA            29051..29123
                     /locus_tag="ACHA6A_001208"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29084..29086,aa:Thr,seq:cgt)
     gene            complement(29242..29880)
                     /locus_tag="ACHA6A_001209"
     CDS             complement(29242..29880)
                     /locus_tag="ACHA6A_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8969420.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10193 a         5288 c         6089 g         8394 t
ORIGIN      
        1 aagccatttt cacaaaattt ttcttactac tcgaaactaa gcgacttttt cacaacctct
       61 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
      121 ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac
      181 ctctttttat aagagttttt actcattaat ttacaaaaaa caagcaaaat gcgaattttc
      241 tcacattttt tttaacaaaa tcaaagttat tgcttgaaag aagaagaatc catcgttaaa
      301 ataaagcttg acgtagaggg gagcaagaaa tatgaaagat tttcaattgg gggctgccac
      361 ctatttaaaa agtgtagcca ttcgggtgaa agaccgagat aaaatgattg atttttacaa
      421 gaacgttgtt ggctttgatt tgaaaagaga agaaaacgaa ttagccattt taggaacaga
      481 agaagctgga agtgaaatgt tgctgctgga agaaagtcct agagcgaatg atcacatggg
      541 ggtgatcaaa aagctgaacc gtttttcatt gattatccct agcacagaag aaatggcaga
      601 tatcctttgt cgtatccgta aaatgaatta tccaatcaaa gaagctttag aagatcatgg
      661 acgtatgggg attttgctcg tagacccaga agagaatgaa ttggaaattt accatgaaga
      721 gaataagaac gaccaaagag aaattccaga aacaatggat caagaagcat tattaagaaa
      781 atctgaagaa aaattcagta aactatcaac aggttcttat tttgaaaaaa tccatttaaa
      841 tgttactaac ttaacaaaag aacgccaatt tcttaatcat gtacttggtt tgaaggtaca
      901 taatgaagaa gagggcatcc atgtattgaa tgaaggagat ttccatgttg gtttgacaga
      961 agctagcgga ggcgcaattg atttgcctac agatgaagtg attggacttg attttctgaa
     1021 attcatgatc gatactgaaa gcatggaagg actgattgct cacttgcagc aactatcagt
     1081 tgatttcttc ttagacaaaa agcacacagt actcacaatc tatgatgcta taggaatcga
     1141 atggtggttc gtcagtgagc caaaagaaaa gaataaaact gacattcgat gcgacgctta
     1201 actttttctt tcttaacgat tgtcgcatgt ctcgtcattt tatgaacggt catactatcg
     1261 cttattctag agatagaaag agaaaatagt ataccggggg aaactgcgcg gtttttcgcg
     1321 cagttttttt gtgtaaaaaa ggttgattcc tcatagatta agctttaagc ttggactgta
     1381 agaatctgat agaaaagcag gagaaaaaga tgtatccaca cacgaaacag cttattttga
     1441 aaaagatgga agaaggattt tatccaggtg tagcagtcgc cttcataaaa gaaaatcaag
     1501 tagaaacatt tacagccggc tttgcccaag tcgttcctga aaagcagctg atgacagaag
     1561 atctgttgtt tgatgcagcc tcactgacga aagtcgttgc aaccacttca ttagttttac
     1621 gtctattaga agatggaaag atagaactcg atcaaccgct tcataaatat cttcctgaat
     1681 ttgaaaatca gacaatcact ttgcgtcatt tactgacaca tacttctgat atcaaaacat
     1741 ggataccaaa cagagataag ctatctcagg aagaattgat cgccgcttat ttgaagctag
     1801 aaccaggtga gcaactagga aaagaagtaa aatatacaga tgccggtacg attcttcttg
     1861 ggttgatgct agaggaaatt taccaagaaa ctgcagtgga aatctttcaa gaacaagtat
     1921 tggaaccatt aggaatgatg aacagcttat ttcttccttt tccttccaaa aaaatcgttc
     1981 ccacagaaca actgccttcg ggtgaggttt tgcgtggaca aactcatgat ccgaaagcaa
     2041 gagttttggc tgaacatgca ggtaacgcgg gtctctttat tgatttgaaa gacagcatta
     2101 cttttgtcca aatgtatttg aacaagggaa aaatccgtaa tggtcgtttt ttagaagaag
     2161 agacgattga ttctttactg caagatcaaa caccgaaaaa aaatggctat cgttctttag
     2221 gatgggactt aaaaaaggat gtagaagacg gtcatgcgat tctttttcat actggttata
     2281 caggtacgtt tttcttgatt gatataaaaa cacaagaagc gttgatattt ttgtcaaatc
     2341 gcgtacatcc tgtcgatcat cgtgcggagt atctcatatg tcgagatgaa attgttgaaa
     2401 actttttgaa agagaaagcg gtattaccga aataatgcta taatagctat gaaaaatgag
     2461 aggagtttga attatgcagg aacctatgtt tttaaaacca gtttttcaag aaaaaatttg
     2521 gggaggaagt cgtttacgtt cagtctttgg ttttgatatc cctaacgata aaatcggaga
     2581 agattgggcg atcagtgcgc atcctcatgg tgtcagtgta gtagaaaatg gcgaattcaa
     2641 agggaaaaga ttagatgaac tatggtcaga acacaaggaa ttgtttggcc atccttcaga
     2701 accagtgttt ccattattga tcaagatctt agacgcagaa gatgatctat ctgtacaagt
     2761 acatccagat gatgcctatg ggatgaaaca tgaaggtgaa ttaggaaaaa ctgaatgctg
     2821 gtatatcatc gatgctgaac ctggtgcaga aatcatctat ggtcatcatg caaagacaag
     2881 agaagaacta gctgagatga tcaaagatgg gcgctgggat gaccttttga agaaagtacc
     2941 agttaaaaaa ggtgatttct tttatgtacc aagcggaacg atccatgcta ttggtaaagg
     3001 aatcatgatt ttagaaacac agcaaagttc tgacacgact taccgcgtgt atgactacga
     3061 cagaaaagat gcgcaaggac aaacaagaga gctgcatatc caacaatcga tcgatgtaac
     3121 aactgtccct gcgaaaacac cagaattaca aatcaaagaa gtccgaaaag ggaattcatc
     3181 gatcgtcact tatttagaaa ctgagttttt caatgtatat gagtgggata ttaaaggaat
     3241 cacatctttc aaaaaacaag ccccttatac attgatgacg gtaatcgaag gagcaggaga
     3301 ccttgtcgtt gacggaaaaa catatccatt agaaaaagga acaagctgta ttattccaaa
     3361 tggcgtttct gagtgggcgg tccaaggtga actagcaatc attgcgtctg aaccaggcaa
     3421 atagttccca gatagctgaa agcagcagac ggtttttcgt ttgctgcttt ttctacataa
     3481 aatccgtcca tcaataaata gaagtctggt gaaaaagatg gaatacaaat ggaaagcaaa
     3541 caacagcagt cgcgtgtcta tgaaacaact gagaaaagct ggacatcact taaatgaaat
     3601 gatcatgcca aatctaagtc tgtttatcgc ttggggaatc ttgtcattag caacaaaagg
     3661 tgtgtctggc gatttgcaca ccacattaga agaggtatgc cattggatga tccagctgct
     3721 tttacccatc ttgatcagtt attcaggcgg gaaatcggta gggggacaaa aaggggcagt
     3781 cgccggtgca attgcttctt tagggttgat cgctcgttcc aatgctccac aaatcgtagg
     3841 agcgatgatt atcgggccgc ttgccggcat cgtatatgaa caatttgttt ctagattcca
     3901 tgtgaaattc aaggctggat atgaaatgct tgtcagcaac ctactagtag gactgactgg
     3961 tagcgttatt tgtataattg gaatcgtttt tattgaacca gttttaaata gtgttcactt
     4021 gttgctcgcc tccgttgtgt cttggctagt cacaatgaat ctgttaccac ttgtcagtct
     4081 gatcttagaa cctctaaaag tactattttt caataatgca atcaatcatg gtgtattgac
     4141 acctttagga atcgaattct cacaaacagt cgggtactct tccttatttt tgatggaggc
     4201 aaatcctgga cctggtttag ggatcttact ttctatcctt tgttttgcag aaaaacagga
     4261 aaaagtaaat gcgagtggag cattgatgat ccatgcgatt ggcgggattc acgaaatcta
     4321 ttttccattt gtcttgctgc gaccgtatct atttttagct gtaatggctg gtggggcaag
     4381 cggcacgttg atatttcaat ggttagatac gggattagct gcacctgtat cgcctgggtc
     4441 actactcgtg atttttgcca atgcgcctta tccacaacta gctggggtaa gtatagggat
     4501 agcaatcagt acatttgtga ccttcatttg tgcagcgata ctattgaaat taccaaataa
     4561 agaaaaagag aacaaaacgg tcaaagaaag gggagaaatc ttggaaccat ctatcattca
     4621 ttccgtcatt tttgcttgcg actcaggtat gggatcaagt gctatgggag ccgccctgct
     4681 gaaaaagaaa ttagcagcag aaaaaatcga tctttctgtc ggatacagct ctatctatga
     4741 attgaataac caagtaaatc agctggttat cgtgcaacat gaactagcgg gtcttgcaag
     4801 agaaaaagta cctcaaagtc aaatctatgt actggatcac tttttggaaa cagatatctt
     4861 gctgtcacga ataaaagaag cgatccaatt tccaaagcgg gaagaaaaaa aagagatagc
     4921 agatatcaca gaacaaaaaa cgtcgcctgc taatattgta tttttgtata aaaacaacag
     4981 acgcggctca caaactatgg gcatgacgat tttaagtcaa ttagcaaaaa aagcgaaaaa
     5041 acagtttgtt atctcaaaag aatcaataga aaaaatcaaa atccaagaag aaacgattta
     5101 tatcgcacaa agagaactag ctgtaactga acagctagaa gaaaagatcc ctcggctagt
     5161 tctagttgaa cattgggtag ctacgaatga atacgaaaaa ctcgtgaagg gagaaatata
     5221 agatgtattt atcgcccaga gaaaaacaat tattagcaga atttctcagt agcccgtcac
     5281 ctgtatctat ccaaaaaatg atgaatttat tgaaggtcag taaacggact atttaccgcg
     5341 aattagataa tcttgaagtc tcattgaaat cggtaaatgc ttctttgaaa aaagcagcta
     5401 gaggctcatt tgtcattgag gcatcagaag aagtattaga gcaattaaaa gaaaatacag
     5461 atgggaaaag taatgactta tccactgcta aacgacaaca cgcgatttta gccgagttgt
     5521 tactgacgac agagccgttg agtcttgctc atttcatgga atcatatcgc atcagtaata
     5581 ccacttttta tgcagatatc aaacagttgg aagaaagtat tcgccaactg cctttggaaa
     5641 tcattcggaa ccagggatac gagatcgaag gaccggaaaa ataccgccgg cttctgacag
     5701 cgaatgtatt agaattagaa atcaatgaat atgaactttt tcatagtatt tctttcgatg
     5761 catcactgaa ttattttttt cgattcgttg atccgcaaca tctatctctt gcacgcaaag
     5821 taatcgggga agaattgatc caaaaaaaga ccaatctaag tgatagaaag cttgaacatt
     5881 tggttttgat gctgacattg acgatggagc gtgtgacgaa aaatcattta ctgacaaatg
     5941 agacatataa tagtctggca aacaaagaat tactacagac agctaaacga ttgttttcac
     6001 ggatcgcttc tgagacaaaa caactttatc cagtaaatga gatcgtcttt tttgcaggat
     6061 tattaaatga cttcacgaac tcatttgatc agaatttctt tgaagagacg ttcgatacac
     6121 agcttgctta tttagtaaaa caactgattc aagaagtaag tgaagagacg aatgtccatt
     6181 tttatgaaga tgaaactttg tacaaaatgc tgttgactca tttatctggt gtgttttcaa
     6241 gagccgtttt acaagaagag acactgacca atccgatttt agaaaaaatc atggttcaat
     6301 acgaggaagt tgcgaatgca atccgtaaag cagtagagcg gatttttcaa gaaaaaaaat
     6361 tatccgaaga agagatcgct tatatggtcc ttcattttgc gaattcattg gaacgaagtc
     6421 cgaaagaaag caatatcagt attgcaggat tttcgccaag tggattagct tctactagca
     6481 tgttggaact acgattgaaa aggtattttc ccttcatcca acaaattcat ttctatcgga
     6541 ttgctgattt aaaaaaaatc gatttacaag aagaatatga tgttgttatt tctacatcta
     6601 ttttaccagg atacggtgga aaatatattt tagtctcccc gctactgcta gaagatgaag
     6661 tcaagcagct aaaagaagct ttttcgcata ttgagaagaa aaaaaggcaa aaaattacgt
     6721 ataacagcaa taacgagtgg atgatagaag atacttatga ggaaacaatg catttcatgg
     6781 atcaaatcaa tcaattactc ggtcgttttt ttatccaaga actggataat cctgagacga
     6841 tttccgaaac gattcgcagc ttatttcctt attttcctga aggactagta acggatgaag
     6901 aggtggttca tcgccggctg cttaagcgat atgaacaagc acctatcggt attcctcata
     6961 caaatatggg gttattccat acatccagct cattgatttc taaaccgatc ttttgtattt
     7021 tcaatttgaa acatccgttg accattgaag ggatggatag acagccagtc gatttgtccc
     7081 gtatgttagt tatgctggcg ccttcgccaa tccaagaaaa agacgggatt cttttaggaa
     7141 agatcagtgg agcaatcatc atgaatgact tgaacaccga gatcttccgc tcaggaaata
     7201 aagaaatcgt ttatcagttg ttagcgaaaa tcctgattga agaaataaag aaatgaaggt
     7261 gaaaagatga tacagctacc agcagaaaaa atattgctcg atcaatccta tcaaaattgg
     7321 aaagaagcgt tgaccgagat cggccagtgg atgacccaac aagggggaat caaaccggca
     7381 tatatccaat caatgatcga aagacaagaa aaagcgagtg tctatatcgg gaatttcgta
     7441 gccttgccac atgggactga caaagatgaa gaagtagtag aagaaggaat ctatttagtc
     7501 caagtgcctg atggtgtgga ctttggaaca aaagaggaac caaaaatcgc gacactctta
     7561 tttgtagtgg ctttaaaaaa agaacatcag ttagcggtgc tccaagattt agcctttttc
     7621 tgttcagaca tcgaccaagt aatggcgctc agtgatgcgg aaaccattga agaagtccaa
     7681 atgatttttg aacatggaca tatataacca atgttcacag taaaaaaagc tgccttgcat
     7741 caagtaataa cttcaaccaa gataaaaata agaggagcta tttttgttgg gaggttctta
     7801 ttactgcgga tgcaagcttt tgaaaactat ttattctaga aaaccgttta tttgaaatag
     7861 gggacggtga caaggttttt gtcaccgccc ctattatttt tgtgttttaa tagctctttt
     7921 ttatatagat agtgcccata cctatgaata ataaaacgat actccatata ccaagtgtca
     7981 agataccatt catgaaagta acctgttctg aagctagtgg cgagaaaata tctcctccta
     8041 tgatcacatt ttgaaaatca acataagaag ttggcaagta atgactgatt gattctatcc
     8101 atccaatcga agaaaacaag cgatactctg ctaagaaaag acaaagaagt gtacctaaaa
     8161 gattgactag agaatgttca aataaagcag atagaagaaa actgattgta cttaaaaaga
     8221 gcacccacaa caacaggaac agaaagctct ggaaaagata ctcatacatc ggcaggattc
     8281 gtatctcctg accaatgatc gttcctacag gataacggaa gtctcccaaa ccatttttga
     8341 ttattactag tatagagacg atcaaaaatg gcatcacgag acttagcata gctgcaccat
     8401 aataaatggt aaaacggatg ctatgtttct ttaccatact atccagtaat agattgacta
     8461 gagagattac ctgctttctt ttttcataag agatgatgga agaaagaaac aaggcggtta
     8521 tacataaaat caaaaaacta gaaatcattc ctgaaaaaat attttcgtag taatttgcta
     8581 aagagagatg cgctggcaag tcaaaaatag aatatctttg gatctgatgt tctttcatat
     8641 aacggagcaa ctcgacacgt ttttgttgtt caatgattgg aattccttgt ctttgaccag
     8701 aaatcagtcg atccagtata tctttttcat aatgataagt tgcttcgacc atttgctgga
     8761 cattccctgt gttttttgca tgtaagattg cttcgatcag tttgttgctt ttctctaaac
     8821 tttccacaaa ttctttctca ttctcatctt ctttcaacgt ttcaatcaaa cgttgcatct
     8881 cttcatagtt atgattcagt tcatcgaatt ctgtataagc aagtttatga tcaaaaaaaa
     8941 gtgggaatgc aaagagcacg accaacaata gaggaaataa aaagtttact ttgtttttta
     9001 gtaagagatt gcgttcaaat gtaagtaaag acatagttac acctcatata atttacgata
     9061 aatagctaaa atctcttcca tatttgagga ttctgtgacg atttctccat tttttagaaa
     9121 acatgcttta gaacaaatcg attggatatt cgcgatagcg tgggaagaaa gaagaatcgt
     9181 cttcccctct ttctgccatt gtctgatgag gctattcaag atatcaatac ttcctgggtc
     9241 taatccgttc aacggttcat cgaataacat gacagatgaa tcactgatca agtaagcagc
     9301 aagcaagaca tgttgtttca ttcctagaga catttttcca atcggcaaag taaggtactc
     9361 agtcatcttc aatcgttgga aaacagtttg gcattctact ttgctctgcc aatagtattt
     9421 gatcaaagtc aaataatcaa cagccgatag atagtcggat aactgattcg ttgattctag
     9481 aaaaaagaaa gaccggcgat aatctgtata agcagtagga gagatgttgt gaagggacaa
     9541 ttgatcataa gttgttggca gcagttgggc aatgccattc agcaaagttg ttttgccaaa
     9601 tccgttcggg gcaatcaaag cagctacttc tcctgattct actaaaagat tcaattgtcg
     9661 aatcacacgt tttttaccac gtttcatgtt catgtttttg atcagtaatg tcatagatag
     9721 gcctccaata taaaatttaa taacataata gaatggaaaa catctaaatt aaatgctaac
     9781 aaaagttatt gtttgcatat tataaaaaaa ttaaaataac agttttattt aaaattgaag
     9841 ttttctgata ccgaaatctt tttattttgc tgacatactc aaacctttgg cacaaagttt
     9901 cgtgccaaag tacgactatt tctcttttta gtaagcggat acaattagag tatcaaaagg
     9961 agagagggag agacaaaatg gaacggacga ttcccaaaaa caacatttcg ctaaaagcga
    10021 gggtacaaaa attaggcagt actttatcaa gcatggtcat gccaaatatt ggagcactga
    10081 ttgcctgggg tgtattaacg gctctattta tcccagatgg ttatttacca aatgaggctt
    10141 tcgctaccat ggtaagccca atgctaactt atctgattcc tttattgatc ggttatactg
    10201 gcggaaaagt aattgctggt gaccgcggat cagttgttgg ggcaattgca acgatgggtg
    10261 tgattgtcgg aacagatatt ccaatgatgc taggcgcgat gatcatggga cctcttggtg
    10321 gatatgccat caagaaattt gatcagctat tccaaaaacg gatcaaatcc ggttttgaaa
    10381 tgctagtcaa taatttttca gcaggtctta tcggttttgg attagcgtta ttaggttttt
    10441 ctgcgattgg tccagttgtc gatgcattga ctcaagcaat ggcaaaaggt gtagagatca
    10501 ttttatctgc ccatttgatt ccattgacaa gtatttttat tgaaccagca aaaatcttat
    10561 ttttgaataa tgcaatcaat catgggattt tgacaccatt gggaactgaa caagtagcag
    10621 aggctggaaa atctattcta ttcttgcttg aagcaaatcc tggtccagga cttggcgtat
    10681 tacttgcttt tactttgttt ggcaaaggag cagcaaaatc ttcagcacca ggagccatga
    10741 tcattcactt tttaggcggg atccatgaaa tctattttcc ttacgttatg atgaaaccgt
    10801 tattattctt atcagttata ggcggaggaa tgtcaggaag tttcattttc caattgatgg
    10861 atgctggact aagagcgcca gcttctccag ggtcgatcat tgcgattcta gcgatgacac
    10921 cgttcaattc ttaccttcct gttattttgg gcgtgattgt tgccgcagct gtttcgtttg
    10981 gtatttctgc agtaattttg aaagcggatg cgaaagatac aaatgaagac ttcgaaaaat
    11041 ctgttcaaga gacaaaggca gctaagcaag cttccaaagg tgcccaaggt attcctcaag
    11101 gagctacatt ctctggcgta caaaaaatca tttttgcttg tgatgcagga atgggatcaa
    11161 gtgcgatggg cgcttcgatc ttgagaaaaa aagtacagga agcagacttg cctcaaacgg
    11221 tgaccaatca agcaatcagc caactgacag atgactcgaa tacattgatc gttactcagg
    11281 tcgaactaca agaaagagca aaacaaaaag caccgagcgc gcgttttgtc tcagtagaaa
    11341 actttttgaa ttcacctcga tatgatgaaa tcattcaaga attaagcagc aagcagaaaa
    11401 catcagtttc tgaaattgag aatgagccag cagaagagtt tctttcagca aacatcaaac
    11461 aaatcaaaga gattctactt gtccacgatg atcgttccgg atcagcaaca atggggatga
    11521 ccgtgctcaa agatatatta gacaagaacc atttaactat cccgttaaga aaagtcaata
    11581 tccaagattt aaaagatgaa cctgattcac tgatcgtagc caaagaaagt ttagcggaaa
    11641 aagtacagat ggctgcacct caatccattc atttatcagt caacagcatg gtcaatccgc
    11701 aaaaatacga atcgattgca caatatttga aacagacagc ttaagggagg aaaacagcat
    11761 gcaaagtttg ccattcgaca tgatcgagct caatcgatct tttaaaacca aagaagaagc
    11821 gatccgctat tgcggtcaga tgcttgtaga agcagggtgc gttgatgagg aatatatcga
    11881 tgccatggta gatagagaca aaatgttatc tgtttatatg gggaatttta tcgcgattcc
    11941 tcatggaaca gatgaagcaa aaaaatatgt gaaaaaatcc ggcatctgtg tcgttcaagt
    12001 accagatggt gtagattttg gtaccgaaga agaagaaaag attgcgactg ttttgttcgg
    12061 tattgcagga gtcggagatg agcatttgca acttgtacag caaatcgcac tttattgcag
    12121 tgatatggac aatgttgtcc agctagcaga cgcgctcagc aaagaagaag taacggaaaa
    12181 tctagcaatt gcttaaggag agagaaaaat gagagcagta cattttggtg caggaaatat
    12241 cggtcgagga tttattggag aggttttggc agctaatgga tttgagatta ctttcgtaga
    12301 tgtgaatcac acgatcattg atgctttaaa tgaaagaaaa gaatacgaaa tcgaattagc
    12361 agatgaatca aaagaaaaaa tcactgtaaa aaatgtcaaa ggaatcaaca atcaaacaaa
    12421 tccagaaaaa gtaatccaag aaatcgctga agcagattta gttactaccg ccattggccc
    12481 gaatatctta ccttttattg ctgagttgat tgcaaaaggt atccaagaaa gagaaaagga
    12541 aggaagcaca acatctttag atatcattgc ctgtgaaaat atgattggcg gttcagcatt
    12601 tctagaaaaa gaagtgtata aatatcttcc ggaaacatca tttactgaca aatatatcgg
    12661 ctttccaaat gcagcagtcg atcgaatcgt cccattacaa caccatgaag atcctttatt
    12721 cgtccaagtg gaacctttca aagaatgggt cattgacgat agccaaagaa aaaataaaaa
    12781 aatccaatta aaaggggttt tatatgtaga tgatctagaa ccatatatcg aacgcaaact
    12841 atttagtgtc aatactggtc atgcgactgt cgcttataca ggtgccttgt taggttacca
    12901 gaccattgat gaagcaatgc aagatgcgtt agttgtggca caattaaaat cagttttgca
    12961 ggaaacagga agtttgctga ttgccaaatg ggggtttgat gcagagcagc atcatgcgta
    13021 tattgaaaaa atcattcatc gcttccaaaa caaaaatatc tctgatgcga tcacacgtgt
    13081 cgctcggaca ccattacgta agttaggtta tcaagaaaga tttacacgtc cagtgagaga
    13141 actacaagaa cataacttga cttgcccaca tttgacggca acaatgggga ttatttttaa
    13201 ttactatgat cctgaagatg aacaaagccg tcaattgcat gaaatgaaga tccatgaaaa
    13261 cttggaacaa ttgattcaag aagtcacagg aatcaatgat ccgaaaacaa tcgggaatat
    13321 caagcaaaac gtgaaccgtt atgccaagca agtcgcttaa aataggatac gaaaaagggg
    13381 aatgtgacga aatttttgtc acattcccct ttttcgaata actaggctgt ttgtccttca
    13441 agatagggca gatccaattc tttaccgtaa cgttgttcta aagctaggcg gatcaaacga
    13501 gctgccaggt tctcatttct tgcaaggata ggtccatgga aataagaacc gaagacattt
    13561 cggtaaatga caccttcaga ttggtcttca ccattatttc cttgaccttg taccactttc
    13621 cccaaaggtc gttcaccttc acctaagaaa gtccgaccat tgtggttttc aaatccataa
    13681 taagtttcat tgaattcttc gttatggata acgatatcac cgataaaccg gttattttcc
    13741 tgacttaacg tgtagtgatc cagtgcactg attccttgga tcttttctcc attagctccc
    13801 atgtagtagt gacctagcaa ttggtagcca ccacaaatgg ctaacatgac gccatcattt
    13861 tcgatatagt tgatcaaaga atctttcttt ttctggatgt cttttgaaat gattaattgc
    13921 tcaaagtctt gaccaccgcc aaagaaaacc aagtcatatt tatcagcatc aaaaggttca
    13981 tgtatactaa cgatctccgt atggcaagaa acccccattt tttctgctat atatttcaac
    14041 atcaaaaggt ttccgttatc accatatgtg ttcatcaaat ttccataaag gtgggcgata
    14101 ttgagttcat aattagccac ggttcattcc tcctttgata aatccttgag cagctagttc
    14161 tttacgaagc tgcaatacag cagtgtaagt agctaaaata tagacatgct cagttggcaa
    14221 ttctttgatt ttttcgatta cttctggtaa ttcttttgtt tctgtcaatt tattttctgg
    14281 aatcccggca acttttagac gtaaagtcat atctgtatga cggtctcctc cagaaataac
    14341 agctgggata tccatatcgg caaagttttc aaaattacca tcccaaatcc agctgacatc
    14401 gattccgtct gcgtaattgg catttaataa agccactaaa gaaaatggat aaggagctag
    14461 accaatcata tcgattactt gattgatacc tacaggattt ttgacaagta ctaatgtaca
    14521 ttttttccca ttgatattga tgacttcttg tcggccgaat actttctcgt catatcctaa
    14581 accttgtttg attttatcag gatcgacatt gaagtactct gccactgcag tagctgctaa
    14641 agcattgtag acattataca taccgccaac ttcgattccg tattcatgac catcgatcac
    14701 gaaatctgct gacacattgt ccatccgtac catatcagtt aatcgataat ccaattcagg
    14761 acgtttgaag tcacaatttg gacagtaata tttccctaag tttgcataag tgatcatttt
    14821 ataatgcaag atgtgatgac aatttggaca taagacaccg tctgtattgt aatgagccat
    14881 ttgttcacga tcttcttcat ggtcaaagcc ataatatttt ctaggattga ctgtttcgat
    14941 agaattgaag attggtgaat caccgttact gataatcgtt gcatttggtg actttgcggc
    15001 accgtctaca atcattttat aggtcgtata gatttctcca taccgatcca tttgatcacg
    15061 gaaaatattt gtaaataaga acagttctgg tttcatatat tctgtgactt tgctcaaact
    15121 tgcttcatct atttctagga cagcaaactt tttctgtcct ttttttggtt ttgcctgcaa
    15181 gaaagtcgat acaattccct gtaccatatt cgcaccagta gggttggtta gaacttcatc
    15241 gaattgctgg cgcaaaatat tgactgttaa agcggtagtc aatgttttac cgtttgttcc
    15301 ggtgacaacg acgacttggt agtcttttgc caaactatct agtatatgtg gatcgacttt
    15361 cagcgctagt ttacctggta aacttgatcc gcctttaaaa aaagttttta atgcccattg
    15421 cgacgttttt ccaactgcaa tcgcaaaatg actgcgtatt cccattatgt aacctccaaa
    15481 aacgatttgc tgacgaataa attcaactaa tcataccata tgttgaaaaa aaggttaaat
    15541 tgcttttctt attcttaaga aaaggttacg gctgaaagtg catgaaatcg gacaaaatcg
    15601 catgatttaa caaaaaaaca gcttgaaaca aaatgattgt ttcaagctgc tgtgatttta
    15661 ttattccctc ttacctatat tcccatttta accgataata attttctcgc ttggatattc
    15721 atagcctaga tctcgtgttt cttttggcac gaataacatc aatgtatata gcattccgat
    15781 ccgcccaata aacatcagtg tagcaatcgt gattttgccc acaacagaca agtctccggt
    15841 aatccctaaa gataaaccag ttgttccaaa cgcagaagtt acttcgatga tgatcgagat
    15901 cagcgtttgt tcttctgtag cagataaaaa aacgatacaa aagaaacaca taccaagaga
    15961 aagcataaag acaacgactg attttcgcac atcatcttga tcgattctcc gtccaaaaat
    16021 attgatgtta tcttcacttt tgaggaagga atatagatac aaaccgatga ttgctacagt
    16081 agttgttcga atcccgccgc ctacagaact aggactgcat ccgataaaca tcagtaaaga
    16141 aaaaatgatc agtgttgtga tttgaaaatc acccaaatcg tgaatctgca gaccagcatt
    16201 acgagtcgta atcgaataaa acatcgaatt gatccatttg acactcatat tggaatcttg
    16261 gaataaatgg tccttttcca atagataaat caagacagtt cccgaaacaa acaagataac
    16321 aaaagctaac acagctaatt tggtaaacaa ggaaaaacga aacggaagct tcgcattgga
    16381 acgtttgtat aatagccatt cccggcactc catcaataca ggaaaaccaa ttcctccgac
    16441 aaaaatcaaa aacatgatca ggatcaagaa aaaatagtca tgtgcaaagg gcttgatgga
    16501 atccccagtg acgtcaaacc cagagttcgt gacagccgat atcgcctgat aaaaaccgta
    16561 aaaaacggca tcacgccaac gatcaaaata gcctctgtaa taaaaataaa tagaaaagaa
    16621 tgtcccaaaa agtaattgaa accagattaa aatagcaaaa gtgatccgaa tcagtcggac
    16681 gataccagac aaacgcggtt gattcatatc cgtcatgatc agctgtcgct gtttcagtgt
    16741 gatccgtcgt ttagaaaaaa tgacaaaagc agtagaaatc atcatgatcc ccaaaccacc
    16801 aacttgaaat aatatttcca gcagtataat accgttatca ttgaacacag aattgatatc
    16861 aaaagtcgat agtccagtca cactcactgt actgattgcc atgaacagca tgtccacaaa
    16921 agaaacatgg ctatctggtt cgcgaaaaat cggcatataa aatagaacga gtgataagac
    16981 cgtcatcaat atataataaa aaacgatgat ttggatcgat gagaaatgat tggcagcaaa
    17041 cttctgtcct cgccgctgca gccttcgaaa gaataaatca acatgcacag tactatcctc
    17101 ctgacaatag tcgtttctac cagtatacaa ggggaattag gaatactcaa agaaattatc
    17161 aaacagataa caatacagct ttcgattaaa atagaaatgg cttataataa ggaaaatcct
    17221 agaaaaaaga aggaggagga ataacaaatt gattaatgct caacaaatcg tagaaaaaat
    17281 gaaaaatcaa aaaataaatt atgatacagt tctgaaaaaa atgatcatgc aatgggagaa
    17341 agaagaagta aagcctaaga tattaataca tacatgctgt gctccttgca gtacgcacac
    17401 attggaattc atgtctcaat atgcagagat cacgttattt ttttcaaatt cgaatattca
    17461 tcctaaaagt gagtatttgc gaagattaca tgaacaaaaa cgatttgtag accaatttaa
    17521 tgagaaaact gaccatcatg ttgcgctaat cgttgatgaa taccgtccaa atgaattcac
    17581 caaaatggtt ttaacaaacc atcttgaaaa cgaaaaagaa ggtggtgctc gctgcagtgc
    17641 atgcttcaat atgcgattag atcttgttgc gcaaaaagcg caagaactag gatacgatta
    17701 ctttggaagt gccctgacca tctcgccaaa gaaaaatgca gctttgatca atcagatcgg
    17761 aatggatatc caaaagattt acgggacaca atacttgcca agtgatttca agaaaaacaa
    17821 aggctatgaa cgctctttgc aaatgtgcaa agagtatgat atttatcgcc aatgttattg
    17881 tggttgcgtg ttcgctgcca agcagcaagg atgcgatctg aaagaaatca atcgggaagc
    17941 aaaagcttac ttaaaaacat caaccgtagt ttttgaggaa aattaaagaa atctttcgga
    18001 aaaacgtaag aaaaaaataa agaatttatg gtagaaataa ggtagactgt ataatttgtc
    18061 cgtagtaaag taaggaaaca agaataaatt tcaatgaagg aagagataag atgcgaaaag
    18121 tagcacctat tttgaagcca atcgctgtat taacaatctc gacgattgtc gcgactacta
    18181 caggtattgt gaatagttcc agctaccgtg caccaagttc aagttctagt actgtagaag
    18241 aaacaacacc aactacttca gtctcgacat ctgaaccaac aacaacggaa agtttgaatg
    18301 aaaccactac gacaagtaca tgggacgata caacaactag ttcgtcgtat gatgagacat
    18361 atacaactga cagctcaagc gaaacaagta atacggtacc agatggcact ccatcggatt
    18421 caaccaatcc aagtacaagt ccggatacaa gtgctagctc cgaaacgata ccaagcagta
    18481 cgactgtaga tgataacacg caaaatgatg atgcagcacg tacagcacag caaaaccaag
    18541 attcaggaaa tacccaaacg tataaccagc aagcaggtga ttctcaatga catttttact
    18601 agaccttatc aacggggtgc ataaattcct tggctattta gacatcagtc ctaaatatct
    18661 taaccgtgga tatacgattt taagtgtgat tcccacgctt tatttattga gaattgttta
    18721 tggactttgg caaaaccaaa actatcttca atttttctta tatggacttg catttctagt
    18781 gcttgtttat tttactgttt taaatgtatt ttattacttt ttagataaaa acacgaaagc
    18841 cgatgtcact cagctttttg tgaagtattt acctgatgaa gcgttcaaca tccagacaga
    18901 agaatcgata gataatggaa attcgatcga taaagtgaat acacaagagg tcatggtttc
    18961 ttatgacgaa gattatcaat tgaaattagc cgagaatatg cggtatttga ttggaaatgg
    19021 tgagatcaaa acaaatgaac tcggtcgcat ggatggattt ctggtcgata gaaatacatt
    19081 gtatccttat tactatgtca agaaaacagg agagaaagag tatgttctaa atattggacg
    19141 aagctactcg gacatggaaa aagtagggac cattcgactt gactcaacag aagacacatt
    19201 ggaacctgtc ggattgttca tcgtcggcgg tgattttgtc aaagacgggt tacgctatca
    19261 tgagccttat cgtctgaaat tgattgcaaa aaaggcacag tcgcaggaag aaccagtggt
    19321 ttatagtcgt agccaaagaa gaagacattc ataatgtgat gaaacgccgt gtatcagtgg
    19381 ttgcgataaa aacgtttatt cggaataagg gggatgtgac acgtcttttg tcacgttcct
    19441 tttttatata acgagaaaga taagacgaac acatcactta ttctttcctt tcatttataa
    19501 acattgtaga ataaagaaag tacaacgaag tattgtgaaa aaacgtaata aataacgagg
    19561 aaaatacaag taggaggtaa gctgtgaaag aatttttgaa aaattggggg attttgatcc
    19621 ttgtattagc ggctatttta ttagctagag tgtacgtgtt tacaccagtt actgtaaacg
    19681 ggcattcgat ggaccctaca ttaagtgatg gacaacgatt gatttcatct aagatctcaa
    19741 actatgaacg aatggatatt attactacaa aggaaccggg ggatgaagaa cggatgattg
    19801 ttaaacggat catcggtatg cctggagata cagtcaagat ggaaaatgac cagttgacca
    19861 ttaatggcaa aaagtatgat gaaccttatc tggatgagtt caaaaaagaa ttttcagaag
    19921 ataagttaca aggagaatat gcttatagct caggattcca agcacaagca gaaagttcat
    19981 ccacctttac gagtgacttt gaatatactg tgccaaaagg aaagtatctt gttctaggcg
    20041 ataaccgctt gatttcaaaa gacagccgaa tgtttgggtt agtcgacaaa gatatgatcc
    20101 aaggaaaagt tgttttccgt tattggcctc tttcagaaat taaaatcatg tgatgaaaaa
    20161 ataaaaagaa gctgtgacat gttttttgtc acagcttctt tttccgaaaa ttccttctta
    20221 tttcttggag ttgctcactt tttttgcaat ctcattttaa gctttttggc tgatggataa
    20281 gaaaagcgaa gcgtttttgt tcataaatag cttttcccca cgatggaagg tatgactagt
    20341 agcagagaca aagcccgttt tttccaatcg attattcaat tcttcttggc taaagccatt
    20401 atggactttc ggatgactaa tttgttcatt cttgtcaaag tctacgatga tcaattgacc
    20461 gccaggagct aaaatttcat aaagttttgt aagaatattc tccgtatcag gaatgtgtaa
    20521 aagtacaagt gatagaagga tgagattagc ttggacagca ggaaattcga cagaagcatc
    20581 agcgtggatc gttcggacat ttttcagatc tgctgcttgg atcttttctt ctgccatttt
    20641 cagcatagtt tctgaagcat cggcaatgat cagttctttg aatctttccg caagcggtaa
    20701 actaacaagc ccagttccgc ctccgtaatc cagcaggact ttttggtggc tgtcgcgcgg
    20761 tagataagta gttagttctt cgcgaataat cttggctaat gcttgacggt caggggaatc
    20821 ataatggtta gcgatggaat caaaaatatt ttttgtcatc aaatagctcc ttacgtaaaa
    20881 ctttgaaatt ttaacttatt atagcacgtg aaaagaaaga aagcatgcta tactcaattc
    20941 aaaaagggag aatctagaaa gaatatgccg atatcgtttg taaaagatag agaggaaaaa
    21001 ggaaagtgtg tccgagaaat attactagac ttgcctgagt ggtttgggct tcctgaatca
    21061 acggaaaaat atatcgagga atcaagtaaa ctacctttat ggtgtgaaaa aagaaaagaa
    21121 gaatatttag gctttatcac tctttcacag acaagtgaag atactgccga aatctattct
    21181 attgtatggg aataaaaaaa gactatcatc accaaggatt aggaacaaaa ctttttaaag
    21241 aagcagaagg ttacgcagca aaacattaca aatatttgca agtgaagact gtagacgagg
    21301 ggcactactc tatatatgat caaactattt gtttctacga atcattagga ttttcaaggt
    21361 tggaagtttt tccaaattta tgggatgaat ggaacccatg tttagttctt gtcaaaaaat
    21421 tggaacaaaa gtgagcaata gatttaatac taatctgtgc ttgatgaaaa ttctcactgt
    21481 aaaaaatgct gaaaatcaag tataatacca taggaacaaa ctggaaggtg aaaaagagca
    21541 tggcacaact attttttaag tatggcgcaa tgaatagcgg caagacgatc gagatcctaa
    21601 aagtcgcaca taattatgaa gaacaaaata agccagttgt cttaatgaca agcggtattg
    21661 atacaagaga tggtttaggc gttgtttcaa gccgaatcgg actgaaacgt aatgcgatgc
    21721 cgatatttga agaaacagac gtatttgacg taattcagaa aatggaaagc aaaccctatt
    21781 gtgtgctgat cgatgaagcg caattcttaa aaaagaaaca tgtcattgct tttacgagaa
    21841 ttgtggacga attggatatt ccggttatgg catttggttt gaaaaatgat tttcgaaacg
    21901 agttatttga aggatcaaaa tatttattgc tttatgcaga caaaatcgaa gaaatgaaaa
    21961 caatctgttg gttctgccac aaaaaagcaa tcatgaattt acattatatt gatggaaacc
    22021 ctgtttatga aggcgatcaa gtgcaaattg gcggaaatga agcttattac cccgtttgtc
    22081 gaaaacatta ctttcaccca tcaatgatta cagaaggaga ataagcatgt acgatcaatt
    22141 acaagcaatt gaagaccgtt atgaagagct tggagaactt ttaagtgacc cagaagtgat
    22201 ttctgataca aagcgtttta tgcagctttc aaaagaagaa gcaaacacgc gcgaaacagt
    22261 cgaagtttat cgtcgttata aagaagtagt acaaggaatc accgatacag aagaactgct
    22321 tggtgaaaaa ctggatgatg aaatggctga actagcaaaa gaagaattat ctgaattaaa
    22381 aaaagaaaaa gaagtcttag aagaaaaaat caagatcttg cttttaccaa aagatccaaa
    22441 tgacgataaa aatatcatca tggaaatccg tggagcagca ggcggagacg aagcagcatt
    22501 attcgctggc gatcttttca atatgtacca aaagtatgct gaagcacaag gctggaaaac
    22561 agaagtgatg gaagctaacg ttaccggaat cggtggatat aaagaagtga tcatgatgat
    22621 caccggagac aacgtatatt cgaaattaaa atatgaaagt ggtgcgcacc gcgtccagcg
    22681 tgttccttca actgaatcgc aagggcgtat ccatacttcc accgcaacag ttgtcgtgat
    22741 gccagaagct gaagaagtag agatcgatat tgaagacaaa gatatccgta cagatatcta
    22801 tcatgcttca ggtgctggcg gacagcacgt caataaaaca gcatctgctg tacgtttgac
    22861 tcacttacca acagggatcg ttgttgctat gcaagatgaa cgttctcaaa tcaagaaccg
    22921 tgaaaaagca atgaaaatct tgcgtgctcg tgtgtacgat aagatccagc aagaagcaca
    22981 aagcgagtat gatgccaatc gaaaatcagc agtcggaact ggggatcgtt cagaacggat
    23041 ccggacgtat aatttcccgc aaaatcgtgt gactgatcat cgtatcggat taactatcca
    23101 aaaattagat cagattttag cagggaaatt agatgagatc gttgatgcat tgatcatgta
    23161 tgatcaaaca tcaaaactgg aagctatgca aaatgggtaa aatgacctat cgagaagtcc
    23221 tgtcacgggc ttcttctttt ttagaggatc atgggaaaga agcatatagt atccaatttt
    23281 tatttttgga acgaaaacaa tggaaaaaac ttgattggct ccttcatatg aacgaagaga
    23341 tttcagaaga agaacagcgg ttgatcgaaa ccgatctgcg acttctgctt gcagatcatc
    23401 cacctcagta tttgctagga tatgcagatt tttatgatca tcggttaaag gtaactgaag
    23461 caacactgat ccctcgacca gaaacagagg agttagtcga atggtgttta gacgagacgc
    23521 ctggtgtacc gttagaagtg atagatattg ggacagggac tggagcaatt gctattagtt
    23581 tgaaagcagc aagaaaaaat tggcatatat ctgctgtgga tctttcagaa gaagcattag
    23641 aagtagcgaa agaaaatgct cagaaagaag gaacgaaaat cagtttttat catggagata
    23701 cattaggacc agtaatggat cagcaatttg atgtgattat ttctaatccg ccgtacatca
    23761 gtcggaatga atgggaactg atggatgaaa gtgtccggtc attcgaacct aaaatggccc
    23821 tttttgctga gaacgatgga ttagctattt atgaaaaaat agcagttgaa gcaccaagcg
    23881 tactaaaatc aaatggaaag atttttttag aaatcggatt tcgccaaggt gaagcagtaa
    23941 aaaaaatatt tcagcaagca tttccagata aaaaagtggc gataaaaaag gatctttttg
    24001 gcaatgaacg aatgatccaa gtttatggtt gaagttgtgg atgaaaaatt ttttatttgt
    24061 ttttttatta taaaaaaaca cttatttgac aaggatgtaa acgacttttt ggtttatcaa
    24121 caagtttatc cactattgac aaatagatta tccacaaaca gtggaaaact tgaggataaa
    24181 ctcagacaaa ataagaatgt atgaaaaagg ggttgtggat aaactgtgga aacgaaacga
    24241 tatgatgaaa cagaattgca agaggcagcc gaagcactga aagaaggaga acttgttgct
    24301 tttcctaccg aaacagtata cggattgggc gccaacgctc ttttaccaaa cgctgtcaag
    24361 aaagtgtttt ctgtaaaagg gagaccgcaa gataatccat taatcgtcca tgtcgcctct
    24421 tttgaacaag taaaagagta tgtggacaac tttcatccgg cagcagagaa gattgtgaag
    24481 aacttctggc ctggaccatt aactttgata tttaagatca aaaaagacac tttgccctca
    24541 gttgtgacgg gaggattatc cacagctgca tttcgtatac cggataacaa aaaaacacta
    24601 gaagtaatcg aactttccgg tgttccgctt gtcggtccaa gtgcaaatac gtcgggaaaa
    24661 ccaagtccaa cgactgcaga tcatgtatat catgatctac aaggaaaaat taccgggatt
    24721 attgatgatg gagctacgcg aattggtgta gaatccactg ttcttgattt aagtgatcca
    24781 acagccatgc ctatgatttt acgaccaggt gcagtaacga aagaacaaat cgaagcagtc
    24841 atcgaatctc ctgttgctat tgatcagcat cttgtaaaag aaaatgaaac accaaaagcc
    24901 cctgggatga aatacaaaca ctactcaccc gatacacgtg ttctgatggt cagagatggc
    24961 gattggtcaa ctgcagttca atgggcaaaa aataagaaga tccgtgcagg tgtcatagca
    25021 agccccgaaa ttgcggatca agtgcggaca gatactgcag ctgtttatat gtataacgac
    25081 aactcagtag aagcggcggc taaaggattg tttgcaggtc taagaggatt agatgaacca
    25141 acacttggat tagatttgat ttttgtacaa gtgtatccag aaactggatt ggggaatgct
    25201 tacatgaatc gcttgaaaaa agcagcagga caaaattatt ttgaaaaatg atcaattggt
    25261 tattgtcgca ttcttttagt atgatacaat gctatcaagt aaatcttcta tcagtgaacg
    25321 aaaaactaag cgtaactaat cgtaatagaa gagatacgaa tatttggcaa tttgtttttg
    25381 atgggaaaaa taaaattgta ggaaaataaa attgtaggaa aataaaaagg agctgttgtt
    25441 tgtggtagat tacaaaacgt ttgaccctga tctttgggct gcgatcgcca aagaagaaga
    25501 aagacaagaa cataatttgg agctgattgc ctctgaaaac tttgtctccg aagcagtgat
    25561 ggctgcacaa ggaagtatct tgacaaataa atacgcagaa ggttatccag ggcatcgcta
    25621 ttatggtggg tgtgaatttg tcgatatcgt tgaaaattta gcgattgacc gtgcaaaaga
    25681 attattcgga gcgaaatttg cgaacgtcca gcctcactcc ggctctcaag ccaacacggc
    25741 tgcttatctc gctttggtag aaccagggga cacgatctta ggaatggatc tttccgcagg
    25801 cggacatcta acacatggat caccagtcaa cttcagtggc aaaacctatc actttgttgc
    25861 ttacggtgtt gatccgacaa cagaagttat tgattataat gtggtacgta ttttagctag
    25921 aaaacatcaa ccaaaattga tcgtagcagg tgccagtgct tacggacgaa cgatcgactt
    25981 tgcgaaattc agagaaatcg cagatgaagt aggcgcaaaa ctgatggtag atatggcaca
    26041 tatcgcagga ttagtagcag caggacttca tcctaatcca gttccttatg cagatatcac
    26101 aacgactacc actcataaaa cattgcgtgg ccctcgaggc ggcatgattt tgacaaacga
    26161 tgaagcatta gcaaaaaaaa taaatagtgc ggtatttcct ggtattcaag gcggccctct
    26221 agagcatgtg attgcgggta aagcagtagc attcaaagaa gcattagatc ctgcatttaa
    26281 agaatatagt gaacaaatca tcgccaatgc aaaagcaatg gtcaaagttt tcaatcaagc
    26341 aattggaacc agagtcattt caggggcgac agataatcat ctgatgttga tcgatgttcg
    26401 cgagttaggt ataaatggaa aagaagcaga gagcattttg gacagtgtga atatcactgt
    26461 aaataaaaac tcgattcctt ttgaaacact aagtccattc aaaacaagcg gtattcgaat
    26521 cggcacgcca gcaatcacta ctcgcgggtt caaagaagaa gatgctgtaa aagtggctga
    26581 actagttgtc aaagcattac aagcaaaaga tgataacgct caactagacg aagtcaaaac
    26641 aggtgtacgc gaattaactg aaaaatttcc gttgcataaa aaataaaaat ttaaattcca
    26701 ttagtaaaca gaaaagttca atgttgggct tttctgttta ttttttggga aaacggattc
    26761 tttgacaata tcggtgatgc tctctagtat atgaggtgta attttagtct tcacactaaa
    26821 tctcagggag gaaaagtcat gactgatcgt atctcgttag aaaaggaagc agtagcattc
    26881 agtgatgcaa atgcgcctca tccgcgtatc tatgaactag caccggaaga aggaagagtt
    26941 ttactagaag aagtacaaga atcacctgtg aacaaatatt ccgtcgatat tgaagacttg
    27001 atggtggata ctggcgaatg gggaaaactc aatattcgtc tcgtacgacc agaagggaat
    27061 atggataaat taccagttat tttttatatt catggagcag gctgggtttt tggcagtgct
    27121 caaacccatg ataaattgat cagagaactt gcggttcgta cgaattccgt cgttgtcttt
    27181 ccagaatata gtcgatcacc agaagcaaaa tatccaacag caattgaaca aaattatgct
    27241 gtgttgcaac agctgaataa attagccaag tcaaaaaatc tggatctcga gaatctaatc
    27301 gttgcaggag attcagtagg tggtaacatg gctacagtca tgacgatctt aacaaaagaa
    27361 cgaaagggcc tcccaatatc caagcaattg ctgtattatc cagtcacaaa cgctgatttt
    27421 gatacagatt cctatcatga attcggagaa aactattttt tgacaagaga aggaatgatg
    27481 tggttttggg accaatatac gacgaatgaa gaagagcgtg ctgaaatcac agcttcgcct
    27541 ttgagagcaa atataaatga actaaatgat cttccttcag caatgatttt aacgggagaa
    27601 gcggatgtat taagagatga aggtgaggct tatgcgcgca aacttcgtga tgcaggggtt
    27661 cctgttacac aagtacgctt ccaaggaatc atccatgatt ttgtcatggt aaatgctatg
    27721 gatcagacaa aggcaacgcg tgcagcaatg gcattatcta cttcttggat caagaaaaaa
    27781 gaataagctt tgcttgtccg caggaaaact tcttttcaaa aacggatttt cttaggaaaa
    27841 atggcgagtt tgactagtca atcacttgat tttgttttac aattaaaaag aaacgacaga
    27901 acaaaggaga gcttaaaaaa tgggcaaatt tcaagtgatc gatcatccat tgattcaaca
    27961 caaactaact attattcggg acaaaaactg cggaacaaag gtttttcgtg aagtagtcga
    28021 tgaaatcgct atgttgatgg catatgaagt atcacgggat atgccattgg aagacatcgt
    28081 gatcgaaaca ccgatcactg aaacaacaca aaaaacgttg tctggtaaaa aagtagcgat
    28141 tattccaatc ttgcgcgcag gtattggaat ggttgacggt atcttggaat tgattccagc
    28201 tgctaaagtg ggacatatcg gtctttatcg agatgaagaa acattggaac cacatgaata
    28261 tttcgtcaaa cttccagaag acattgatgc acgtcagttg tttgtagtag atccaatgtt
    28321 ggcaacaggc ggatcagcaa tcatggcaat cgatgcattg aaagaacgtg gcgcgtcgaa
    28381 cattaaattt gtttgtttgg tagcagcacc tgagggagta aaagctttgc aagaagcgca
    28441 tcctgatatt gacatctata cagcttctct agatgaaaaa ttgaatgaac atggttatat
    28501 cgttcctgga ctaggggatg cgggtgaccg tttgtttggt acaaaataag taaatacgaa
    28561 aaagggtgtg acacaacttt tgtcacatcc ttttttggtc atttttaacg tttgatgcag
    28621 aacagctttt tcacagcttt cgtttgcata taaaaaaacc atcccaaaaa tgcagagatg
    28681 gtcaaattca aatctttttt agttctttgt agttaacgat tactacaccc ttactttgcg
    28741 tcatcaagtt cgcattttct gcagtttcat caatctcaac aacagcggaa tttttcaatt
    28801 gctttgaaat cgttccagtt tgcgacttgc cgtgaattgt gaacattacg tgagtattga
    28861 tcttgaattt ttcaccaatt tccgatggta cgatagttgt atcaaatttt ccaaaagttg
    28921 ccattttcat aaaacctgcc tttcacatca attataccac gttttatgaa aaatatcatg
    28981 aaattaaact aattttccat cggactcttt acaatcagcc aaatagagta tataatttct
    29041 tttggataag gccagcttag ctcagttggt agagcaacgc actcgtaacg cgtaggtcgt
    29101 aggttcgatc cctatagctg gcattaaaag agaagacaga tatatcaagg atttttaatc
    29161 cattgataca tctgtctttt tgtttaaagg aaataaagta tgctttctta gcaatagagc
    29221 atgtattatt tctataaaaa tttaggagaa taaaacgact agcaatatag ctgtgaaatc
    29281 aaatatcaca tgaactgccc aacttgtaat gatcgagttg cttttaatgg cagcttggtt
    29341 gaataacaat cttgccaccc cttgtatcaa aaggatgtgt gctacagtgt gtaaaatatt
    29401 tccattatca taagtactgt aatggataaa tccaaagatg attgctgaca agaaactagc
    29461 gacaggaatg ctgaacttac tagaaaaaat agcgagaaaa tacactgaga acaattcttc
    29521 tccaagtatc atgataggca aaataataaa taataacggg ctgaaagcct catttattgg
    29581 attgcctgtt aaaccccatt tcaaaatttg ttgcaaaaat accgagactg cggcactgat
    29641 tactacattg ataaaaaaat atttggctat atttttgaca ggagcgacag gtttcgagaa
    29701 catttttttc gtatcttttt taccaaaaac aagtgtaaat gctataaatc cagtgatagt
    29761 taaagaaaaa atgctaataa tggatggcgc aaatagcaac cctaacaaac cgagtacaac
    29821 gacaatagtg cccaatatcc cttttttaag ttctattttt tttcctaaaa tgttattcat
    29881 gataataact cctactattc agtttttgaa gatagagtca gtatacatga agagttaaaa
    29941 taggcaatgt ttttttatgg atat
//
LOCUS       DAYYHS010000027        29129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000027 DAYYHS010000000
VERSION     DAYYHS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.027"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHA6A_001210"
     CDS             complement(296..1024)
                     /locus_tag="ACHA6A_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969421.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHA6A_001211"
     CDS             complement(1002..2576)
                     /locus_tag="ACHA6A_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8969422.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHA6A_001212"
     CDS             2805..3743
                     /locus_tag="ACHA6A_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969423.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHA6A_001213"
     CDS             3755..4666
                     /locus_tag="ACHA6A_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8969424.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHA6A_001214"
     CDS             4690..6162
                     /locus_tag="ACHA6A_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8969425.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHA6A_001215"
     CDS             6213..6824
                     /locus_tag="ACHA6A_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8969426.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHA6A_001216"
     CDS             6854..8767
                     /locus_tag="ACHA6A_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8969427.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHA6A_001217"
     CDS             8895..11876
                     /locus_tag="ACHA6A_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8969428.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHA6A_001218"
     CDS             11873..13534
                     /locus_tag="ACHA6A_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8969429.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHA6A_001219"
     CDS             13574..14506
                     /locus_tag="ACHA6A_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8969430.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACHA6A_001220"
     CDS             complement(14898..16445)
                     /locus_tag="ACHA6A_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8969431.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16861)
                     /gene="tnpB"
                     /locus_tag="ACHA6A_001221"
     CDS             complement(16547..16861)
                     /gene="tnpB"
                     /locus_tag="ACHA6A_001221"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8969432.1"
                     /translation="MCGKTDLRKGIDGLASLIQQEYQLELYEDAVFLFCGNRQDRFKL
                     LYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLSIDQPRAILPGKKG
                     VF"
     gene            complement(16890..17084)
                     /locus_tag="ACHA6A_001222"
     CDS             complement(16890..17084)
                     /locus_tag="ACHA6A_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969433.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACHA6A_001223"
     CDS             complement(17310..18512)
                     /locus_tag="ACHA6A_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8969434.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACHA6A_001224"
     CDS             complement(18515..18709)
                     /locus_tag="ACHA6A_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8969435.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACHA6A_001225"
     CDS             complement(18756..19604)
                     /locus_tag="ACHA6A_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8969436.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACHA6A_001226"
     CDS             complement(19809..20159)
                     /locus_tag="ACHA6A_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969437.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACHA6A_001227"
     CDS             20337..21200
                     /locus_tag="ACHA6A_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8969438.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="ACHA6A_001228"
     CDS             21824..22816
                     /locus_tag="ACHA6A_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969439.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="ACHA6A_001229"
     CDS             22832..23716
                     /locus_tag="ACHA6A_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8969440.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="ACHA6A_001230"
     CDS             23765..25372
                     /locus_tag="ACHA6A_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8969441.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="ACHA6A_001231"
     CDS             25373..26857
                     /locus_tag="ACHA6A_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8969442.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="ACHA6A_001232"
     CDS             27034..28026
                     /locus_tag="ACHA6A_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8969443.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="ACHA6A_001233"
     CDS             28078..28947
                     /locus_tag="ACHA6A_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8969444.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         9192 a         4774 c         5874 g         9289 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga
    29041 atctgagtag agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta
    29101 caagaaagtt ctttttacca gatctttta
//
LOCUS       DAYYHS010000028        28633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000028 DAYYHS010000000
VERSION     DAYYHS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.028"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..471)
                     /locus_tag="ACHA6A_001234"
     CDS             complement(<1..471)
                     /locus_tag="ACHA6A_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969445.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNST"
     gene            576..821
                     /locus_tag="ACHA6A_001235"
     CDS             576..821
                     /locus_tag="ACHA6A_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8969446.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            977..1828
                     /locus_tag="ACHA6A_001236"
     CDS             977..1828
                     /locus_tag="ACHA6A_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8969447.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            1861..2538
                     /locus_tag="ACHA6A_001237"
     CDS             1861..2538
                     /locus_tag="ACHA6A_001237"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8969448.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            3026..3991
                     /locus_tag="ACHA6A_001238"
     CDS             3026..3991
                     /locus_tag="ACHA6A_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8969449.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            4013..4225
                     /locus_tag="ACHA6A_001239"
     CDS             4013..4225
                     /locus_tag="ACHA6A_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8969450.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            4300..4776
                     /locus_tag="ACHA6A_001240"
     CDS             4300..4776
                     /locus_tag="ACHA6A_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8969451.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4773..6581
                     /locus_tag="ACHA6A_001241"
     CDS             4773..6581
                     /locus_tag="ACHA6A_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8969452.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            6658..6873
                     /locus_tag="ACHA6A_001242"
     CDS             6658..6873
                     /locus_tag="ACHA6A_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8969453.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            6965..7828
                     /locus_tag="ACHA6A_001243"
     CDS             6965..7828
                     /locus_tag="ACHA6A_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8969454.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            7865..8401
                     /locus_tag="ACHA6A_001244"
     CDS             7865..8401
                     /locus_tag="ACHA6A_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8969455.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            8415..8837
                     /locus_tag="ACHA6A_001245"
     CDS             8415..8837
                     /locus_tag="ACHA6A_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8969456.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            8767..11166
                     /locus_tag="ACHA6A_001246"
     CDS             8767..11166
                     /locus_tag="ACHA6A_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8969457.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            11198..13189
                     /locus_tag="ACHA6A_001247"
     CDS             11198..13189
                     /locus_tag="ACHA6A_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8969458.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            13212..13463
                     /locus_tag="ACHA6A_001248"
     CDS             13212..13463
                     /locus_tag="ACHA6A_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8969459.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            13453..14682
                     /locus_tag="ACHA6A_001249"
     CDS             13453..14682
                     /locus_tag="ACHA6A_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8969460.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            14679..16760
                     /locus_tag="ACHA6A_001250"
     CDS             14679..16760
                     /locus_tag="ACHA6A_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8969461.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            16909..20829
                     /locus_tag="ACHA6A_001251"
     CDS             16909..20829
                     /locus_tag="ACHA6A_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8969462.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            20830..21774
                     /locus_tag="ACHA6A_001252"
     CDS             20830..21774
                     /locus_tag="ACHA6A_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969463.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            21778..21981
                     /locus_tag="ACHA6A_001253"
     CDS             21778..21981
                     /locus_tag="ACHA6A_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8969464.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(22281..22517)
                     /locus_tag="ACHA6A_001254"
     CDS             complement(22281..22517)
                     /locus_tag="ACHA6A_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969465.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(22700..23071)
                     /locus_tag="ACHA6A_001255"
     CDS             complement(22700..23071)
                     /locus_tag="ACHA6A_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8969466.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(23068..24396)
                     /locus_tag="ACHA6A_001256"
     CDS             complement(23068..24396)
                     /locus_tag="ACHA6A_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8969467.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(24357..24686)
                     /gene="mobC"
                     /locus_tag="ACHA6A_001257"
     CDS             complement(24357..24686)
                     /gene="mobC"
                     /locus_tag="ACHA6A_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8969468.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(24944..25314)
                     /locus_tag="ACHA6A_001258"
                     /pseudo
     CDS             complement(24944..25314)
                     /locus_tag="ACHA6A_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25459..25644
                     /locus_tag="ACHA6A_001259"
     CDS             25459..25644
                     /locus_tag="ACHA6A_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8969469.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            25802..25969
                     /locus_tag="ACHA6A_001260"
     CDS             25802..25969
                     /locus_tag="ACHA6A_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969470.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            26587..27246
                     /gene="vanR-B"
                     /locus_tag="ACHA6A_001261"
     CDS             26587..27246
                     /gene="vanR-B"
                     /locus_tag="ACHA6A_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8969471.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            27249..28610
                     /gene="vanS-B"
                     /locus_tag="ACHA6A_001262"
     CDS             27249..28610
                     /gene="vanS-B"
                     /locus_tag="ACHA6A_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8969472.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         7365 a         7300 c         7597 g         6371 t
ORIGIN      
        1 agttgagttc gtgatttcat cttgggtctc attaaaaaag ttatccgaat aatcgcttat
       61 ttttgaaaag tctttattgg caccagcctc aaaaaattgt tcaacttttt cagtataaag
      121 ttgtgctaac gctccttttt caatattaac tttaatattt tcatgtctat caatttgtgt
      181 gctgttcagt aagggaagat cagcttttag ttttatatcg tcttgatttc tttcaatttc
      241 aattggcaaa atcttttcag tagattcagt ttctgataaa ggaacatcta gttttttatc
      301 atgataataa gcttcaaatc catctggaac cggaaaaaaa tgtattgggt aattcttttc
      361 tgaggttgtt gtactagttt ccgtacttgt agaacttgtg tcagaagtgg tacacccgct
      421 tatagttccg atgagtaaag cgcaagttag aatgattgca aatttcttca aaaaaattcc
      481 tccttaaaat tgaatatgga ctaatactag tcccattgta gcataccgaa tctttaagat
      541 taataagaaa aaggactaat tttagtccaa aaggtgtgct attcttgaaa gaggaaaaaa
      601 ttattgataa agaaaatgta atcggtagca atattagacg agtccgatta gaaaaaggaa
      661 ttggtcaaac cgagctgatt agagacctac agcttcgtaa aattgctata actagggaaa
      721 cgttggttaa gattgaaggt ggccgccaac acattaagtt ggatcaattg aaagccatca
      781 aagatattct acaggtgtct tatgaagatt tgcttcaatg actacttgta gtcatttcgt
      841 ttgaaattat tatgttacac ccttatgtgt cacgaagaaa tactggtata ataatgaatg
      901 ggatatgtaa cgatagggta tactgttatg ttgcctaata aattgtggat caaaatacaa
      961 gaatggaggg atatccttgt ataaggcata tgaattggat tttacaaatg ctgacttaaa
     1021 tgcattctcc tcaagctcgc aatcatatac acatgtagta aatcaaatag atcacaacca
     1081 aaaatctatt aacaagagaa tagaaaatct atttgaaagt gaaaatgatt tagttgatta
     1141 ttattctaag gacagcaaaa ttctagatgc agacgaaatt attaaagatt ggtttccaac
     1201 tataaaagca gatattttta tatctcactc tcatgctgat gaaaaattag ctatacgttt
     1261 tgcatcatgg ctatttgaaa attttggtct tactgcattt attgattctt cggtttgggg
     1321 atacagttct gatttattaa aaaaaataga ccaaaaatat tgctataagg aacaaacaaa
     1381 aacatatgat tatgataaaa gaaatgtaac aactagtcat gttcatatga tgctttctac
     1441 agctttaaat aatatgattg atagtacaga atgtctgttt tttcttaata cacctaattc
     1501 aattagtttg agcaatgaaa tcactaatga acagaaattt acgtattcgc catggctata
     1561 tagcgaactg acgacagctt caatagtgga aaaaaagaat ccacgattgg aaagtaatcc
     1621 tcaaatgagc accgaggatg ttagaagcat tataaagcac tattcttcgc aagaaaatct
     1681 gcaaataaaa tattctttaa atttaagtga attaacttat ttacgaaaag ctgatttgaa
     1741 aaaatgggct ttagatgctg tggaatgcac taaaggaagt aatacattag atattttata
     1801 taaaaacacc attttcacta atctctaatc aatttactga atcgaaagga accgggtaga
     1861 atggataaga aagttttaat aaagaggttc tctgaagcta ttgaaaaagg aaatgcagca
     1921 atatttgcag gtgcaggatt atcgatgtca caaggatatg taagttggcc ggaactactt
     1981 aatgatcctg caacagaaat cgggttggat tctaaaaaag aaacagactt agttactctg
     2041 gctcaatatt ataaaaatga aaatggtgga tcaagaggaa ttttgaatca gatacttatg
     2101 gataattttg gtgaagaatt agaaatatct gaaaatcatc gaatactagc aagtttacct
     2161 attgaaactt attggacaac taattatgat catctaatag aaaaatcaat ccgagaagct
     2221 tataaaaacc ctcaggttaa gaaaaattat actcagttag caacaactaa tccaaatgtg
     2281 gatacaattg tttataaaat gcatggtgat atagatgatg tcagttcgac agtaataaca
     2341 agagatgatt atgaaaaata tgatgattcg tacgcgttat ttaaagaaac tttaaaagga
     2401 gatttgttaa caaaaacttt tctcttttta ggatttagtt ttacagaccc taatttagaa
     2461 agaattttat cagatataag atgggttttg agagaaaacc aaagacctca ttattgtata
     2521 atgagaaaaa tattatagaa aaaattttag gttatcgctg aaagccccgg aaatacgggc
     2581 atttcgcagg atatgagaac tcaatttact accaatttac tactttcgga ttgagccgca
     2641 tatatgctga accatactgc taatacccgc caccccaaat acggcacatc ataaattgaa
     2701 tacgacaccg taaacgcggc gttcctcttt cccgtcatgg gagaacagga cgccgctttt
     2761 ttatgcccaa atagagaaag gaggcgacgc catgctattt acttccggcc ctgaccgggc
     2821 gtttgaacgg ctgatgcagg aaaagcccgg tttcgatcac tacgacggcg gcggggcgga
     2881 cgcgccggag gattgcggca cctgccgatt ttaccgcccg gattggaaat atcaattctg
     2941 ccagtttgca gagtgtccct atcagccggg caagctgacc gactatgcag cggtcaaatt
     3001 cacagtgaaa ggagcgaaca acgctatggc agttttccgt gtggaaaaga acaaaggcta
     3061 tacggtgatg tcaaatcacc acctgcggaa taagaacctg accttaaagg ccaagggcct
     3121 gctgtcgcag atgttgtcct tgccggagga ttgggactat accctaaaag gactgtccta
     3181 tatcaaccgg gaaagcatcg acgcgatcag gacagcggta tgggagcttg aaaaagctgg
     3241 atatatccgg cgtgagcagg gccgtgacgc aaaaggcaaa atggccgata tggtctatac
     3301 catctacgaa cagccggtat tggaaaaccc ggtattggaa aatccaacat cggataaccc
     3361 ggtgttggaa aatccgacat cggataatcc ggcgtcggaa aatccaacgc aattaaataa
     3421 agaagtacaa agaactgact tatcaaaaaa agaaaaaata attacagatg gacaaagtac
     3481 ccattccatt cctatcccat ccccgacccc tgcccctatg gagggcgagg cagcggctac
     3541 gccgccggaa cggaaaagga caggaaagga tgaggctgta cggatttatc gggaaatcat
     3601 tttggagaac atcgagtatg cctatctcat tcaggacagg agcattgacc gtgaacagct
     3661 tgacgaaatc gtagacctga tgctggaaac cgtctgcacc gcccgaaaga caatccgtgt
     3721 cgccggggac gactaccctg ccgagctggt gaaatccaag tacatgaagc tgaccggcga
     3781 acatatccgc tttgtccttg actgtctgcg ggagaacaca acgaaagtcc gcaatatcaa
     3841 acaatacctg cgggctatgc tgttcaacgc ccccagcacc atttccaact actacacggc
     3901 ccttgtcgcg cacgacatgg cacagcccga ttggggaaag cctaaatccg gcctgccgga
     3961 ctattcctgc ggcgagggcg aaagcctgta acgagaaaag gaggacgacc ctatgaagca
     4021 gggcgcattg atttttgacg agcgaacaga ccgttacgac atccgctttg atctggccga
     4081 ctattacggc ggcctgcatt gcggggagtg tttcgacgtg atgatcggcg gcaaatggag
     4141 gcccacccgc attgaaatgg ccgaaaactg gtatcttgtc ggcatccggg ccgacgacct
     4201 ttccggcctg cgggtgcgga tttgacctat atctacctgc ggcgtctggt ggttccgcct
     4261 tagacgccgc ttttgacttt ctgacaagga gggatgcgat tgcaggaaga agttactcaa
     4321 aagaccattg ccctgtccat gaaaacgggc aagctcacgg ctcaagtgct gcaagcggcg
     4381 ctgaaaaagt acctgcaaca ccgggccaag ggcaaaaaca cgctgcacca cggccagcag
     4441 agcctaaagc agcttaaaaa gcatggggct gatctctcca acattgagat caccgaggcc
     4501 aacattggag cgttcaagcc atgcgccaag aaatacggcg tggattttac cttacgaaaa
     4561 gataagacca cccagccgcc ccactacgtt gtgattttca aggccaagga tgcagataac
     4621 cttgatctgg cgtttcggga gtttaccgcc aagacgcttt ccaaggagca gcggccctct
     4681 atccgcaagg ttttgtccgc tgtgaagcaa aaggcggcgg cccagaccaa gcagcgggcc
     4741 aaggagaaaa tcaaggaaag ggggctggaa ctgtgaagcc tgacgtaaag aaattgctga
     4801 tcctgaacct cccgtatctg atttttgtgt acctgtttgc gaaatgcggg gaggcgtacc
     4861 ggctggccgc tggtgctgac ctgtcgggga agctcctgca ttttgcggat ggttttgcgg
     4921 cggcttttgc caaccctctg ccaagcctgc acctgtttga cctgtgtatc ggtatcgccg
     4981 gtgccgtgat cgtgcggctg gtggtgtact gcaagagcaa gaacgtgaag aaataccgca
     5041 aaggcatgga gtatggcagc gcccgctggg ggacggccaa agacatagcg ccttacatcg
     5101 accccaagtt tgaaaataat atcctgctca cccaaacgga acggcttacc atgaccggca
     5161 ggcccaaaga ccccaagaca gcgcggaata aaaacgtgct ggtgatcggt ggcagcggtt
     5221 cagggaaaac gagatttttc gtgaaaccca acctgatgca atgctttccg accacggact
     5281 atccgacttc gtttgtggtg acagatccga aaggcacttt ggttctggaa accggcagga
     5341 tgttccagca ggcgggctac cgcattaaaa tcctgaatac cattaacttt tccaagtcca
     5401 tgaagtacaa cccctttgtc tacatccatt cggaaaagga cgtgctaaag ctggtaaata
     5461 ccctgatttc caacaccaag ggcgaggggg aaaagtcagc ggaggatttt tgggtgaagt
     5521 cggaacggtt gttttattcg gccctgatcg gctatatctg gtacgaggcc ccagcggagg
     5581 aaatgaactt ctcaacgctg ctggaaatga taaacgccag tgaagcccgc gaggacgacc
     5641 cggagtttca aagccccgtc gatctcatgt ttgagcgttt ggagcagaaa gaccccgacc
     5701 attttgcggt gcgccagtac aagaaattcc tgctgtccgc aggcaagacc cgttcttcca
     5761 tcctgatttc atgcggtgcc agacttgcgc cctttgacat ccgggaggta cgcgagctga
     5821 tggaggacga cgaaatggag ctggacacca tcggggacga aaagacggtg ctgtatctca
     5881 ttatgagcga tacggacacg acctttaatt tcattctggc tatgctccaa agccagctaa
     5941 tcaacctgct ttgtgaccgg gctgatgatg tgtacggcgg gcggctgcct gtccatgtac
     6001 gcctgatttt ggatgaattt gccaacattg gccaaatacc caatttcgat aaactcattg
     6061 ccaccattcg aagccgtgaa atatcggcct ctattatttt gcagagccag tcgcagctaa
     6121 aagcgattta caaggacgcc gccgagatca tttcggataa ctgcgatagt gtgcttttcc
     6181 ttagtgggcg cggcaaaaat gccaaggaga tttccgatgc gctggggaaa gaaacgattg
     6241 acagtttcaa caccagcgag aaccggggaa gccaaacgtc ccacggattg aactatcaaa
     6301 aattaggaaa ggagttgatg tcccaagacg aaattgccgt gatggacggc ggcaagtgta
     6361 tcttgcagct acggggtgtg cgcccgtttt tatcggagaa attcgatatt accaagcatc
     6421 cccggtacaa ataccttgcc gacgccgaca agaagaatac ctttgacgtg gacagatttt
     6481 tgactatcac gcgccggaaa cggcagcaag tggtaacgca ggatgaagta ttcgacctgt
     6541 atgaaatcga cctgtcggac gaggacgccg ccgctgaata aacagcggct tttttcattc
     6601 caacaacaaa atcaaaaaat cattatgagc cggaaaccgg cagaaagagg taaatttatg
     6661 gcatttttta attccgcagt tactactttg cagactatcg tgatcggact gggcggcgct
     6721 ctgtgcgtat ggggcggcat caatctgttg gagggatacg ggcaggacaa tccgggggca
     6781 aaatcgcagg gtgtcaagca gcttgtcgct ggcggcggcg tggccctgat cggtgtcact
     6841 ctggttcccc tgctgtccgg gctgctgggc tgatcctggc ctttgtccct gtccaaaccc
     6901 ttaactaatc gcctccgcgt cctcccggcc agaccgggag ggcgcgggaa aggaggtgct
     6961 acccgtgatt agtgaaatca ttgaagaatg gataaaaggc atcctcatgg acggtatcac
     7021 gggcaacctg tccgggctgt tcgatacggt aaatgccaaa gtcggagaaa tcgccgccga
     7081 cgtgggacga accccgcagg catggaacag cggtattttc aatatgctgc acagcctgtc
     7141 ggaaaccgtg atcgtcccca tagccggggc catactcgcc cttgtcatgt gctatgagct
     7201 tatccagatg ataacggaaa agaacaatat gcacgacttt gacagctcca tgttcttccg
     7261 ctggatattc aaatccgcgt ttgcaatcct gatcgtaacg aacacatgga acatcgtcat
     7321 gggcgtattc gacgccaccc aaagcgtcgt caatcaaagc gccggtgtga ttatcgggca
     7381 gacatccatt gattttaaca ccctgatacc agaccttgaa agccaattgg aggctatgag
     7441 cgtgggcgct cttttggggc tgtggttcca aacccttgtt gtgggcctca ccatgaatat
     7501 cctttccatc tgcattttcc ttgtgaccta tggccgcatg gtggaaatct atgttgtcac
     7561 gtcgctgggg cctatccccc ttgccacaat gggcaacgcg gaatggcgcg gcatgggcca
     7621 agggtatctg aaatccctgc tggcgctggg cttccaagcc tttttaatca tggttgtggt
     7681 gggtatctac gcggtgctga tacagaatat cggttccgct tccgacatat ccggcgcaat
     7741 ctggggctgt atgggctata cggtgctgct gtgcttctgc ctgttcaaga ccggcagtat
     7801 atcaaaggcc gtattcgggg cgcattgatc ccaaaatcca agcatacaca ggaggaactt
     7861 gtttatttca acgtacaaca tcatttacgc tgacccgccg tggcggtatg actgcaaaaa
     7921 cgtacagggc gcagcggaaa atcactattc caccatgagc attgacgagc tgtgcgccct
     7981 gccggtggaa cggctggcgt ccaaagactg cctgctgttt ttgtgggcca cattccccat
     8041 gctgccggaa gctctgcggt taatcaaagc gtggggcttt tcattcaaga ccgtggcttt
     8101 tgtatggctg aaacagaacc gtaaaagcct tacatggttt tacgggctgg gccgctggac
     8161 aaggggaaac gcggaaatct gcctgctggc aacacacggc aagccgaagc gtcgttctgc
     8221 ctctgtccat cagtttatta tcagcccgat tgaacaacac agcaaaaagc cggatgtgac
     8281 ccgtgaaaaa atcgtagagc tggcgggcga tttgccccgc gtggagctgt ttgccagaca
     8341 gaaaaccccc ggctgggacg tgtgggggaa cgaggtggaa tgtgacctta tcctgacgtg
     8401 aaaggaggtg ccaaatggct tatgtaaccg tccccaagga cttaacccat gtgaaaagca
     8461 aggttgtgtt tgggcttacc aaacggcaac tgatctgttt ttccggggcg ctggtgatcg
     8521 gcgtcccctt atattttctc acccgtgact atcttaccaa cagcgcggcg gccctgctca
     8581 tggtatttgc catgctgccg ggctttttgc ttgccatgta cgagcgtcac ggccagcccc
     8641 ttgaagtggt gattatgcag atcatccagt gctgcttcat ccagccaaag gagcgtccct
     8701 atcagaccga caacacctac accgcccttg tgcggcaata ccagatggaa caggaggtaa
     8761 aggccattgt ccaaaaagga aatcccaaga gaaaccgaaa aaacaaagct gacccgcgcc
     8821 cagcgaaaag aaattgacgc ggtaatccgt aaatataagg gcgacggcag gccccacacg
     8881 gcgcagcaga gcatccccta cgaggtcatg tacccggacg gcgtgtgccg ggtatcgccc
     8941 ggcgtctttt ccaagtgcat tgagtttgcg gacatcagct atcagcttgc ccagccggac
     9001 acgcagacgg ctatctttga aaaactgtgc gacctgtata actatgtgga cgcctccatt
     9061 catatccagt tttctttcct caatcggaaa gttgacccgg tgcagtacgc caagagcttt
     9121 gagatcgcgc cgcaggggga cgacttcgac gacatccggg ccgagtacac cggcattctg
     9181 caaaagcagc ttgcaaacgg caacaacggc atggtaaaga ccaaatacct gacattcacc
     9241 atcgaggccg aaagcgtgaa agcggcgcga gccagattga agcgcatcgg ctttgacctg
     9301 ttaggctatt tcaagagcat gggggccgta gcccatgtga tggacggctg ggaacggctg
     9361 aacctgctgc atggcgtgta ccacccggac ggcgagatat tcaattttga ctggaagtgg
     9421 ctggccccgt ccggcctctc caccaaggat tttattgccc cgtcctccct gtgcttcggc
     9481 aatgcaaaga cattcggcat gggcggcaaa tacggcgcgg taagtttcct gcaaatcctg
     9541 tccccggagc tgtcggacga tatgctggcc gacttcctca atacggaaag cggtgtttta
     9601 gtcaatctcc atgtgcaggc cattgaacag accaaggcga tcaagaccat caagcgcaaa
     9661 attaccgacc ttgacgctat gaagattgcg gagcagaaaa aggccgtccg cagcggttac
     9721 gatatggaca ttcttcccag cgaccttgcc acctacggcg aggacgccaa gaagctgctg
     9781 acaaagttgc agacccgcaa tgaacggctg ttccagttga cgttccttgt cctcaacgtg
     9841 gccgacacca agcaaaagct gaataacgac gtgttccaag ccgcaggcgt ggcccaaaag
     9901 cataactgtc cccttgtccg gctggactac cagcaggaac agggccttgc aagcagcctg
     9961 ccgctgggcg tcaatcagat caagatacag cgcagcctta cgacttccag cgtggccgtg
    10021 ttcgtcccct ttgtgacgca ggagcttttt cagggcggcg cggccatgta ttacggcatc
    10081 aacgccaagt cccgcaacat gattatgctg gacaggaagc aggcccgttg ccccaatgcc
    10141 ttgaagctcg gcacccccgg cagcggcaaa tccatgagct gtaaatccga gatcgtcagt
    10201 gtgttcttaa cgacgcctga cgatattttt atttcagacc cggaggccga atattacccc
    10261 cttgtcaaac ggctccacgg acaggtaatc cggctgtcgc ctaccagcaa ggactttgtg
    10321 aacccgctgg acatcaacct gaattattcg gaggacgata acccgctggc cctgaaatcc
    10381 gactttgtgc tgtcgttctg tgagcttgtc atgggcggca agaacgggct ggaagccatt
    10441 gaaaagaccg tgatagaccg ggctgtgcgc gtgatctacc ggccctatct ggcagacccc
    10501 cgcccggaga atatgccgat cctgtccgac ctccacaaag cgttgttaga tcagcatgtc
    10561 ccggaggctg accgggtggc gcaggccctt gacctgtatg tgtccggctc cctgaacgta
    10621 tttaaccacc gtaccaacgt ggacatcggc aaccggcttg tgtcctttga cattaaggag
    10681 ctgggcaagc agctcaaaaa gctggggatg ctcattgtcc aagaccagat atgggggcgc
    10741 gtcacggcca accgaagcca aggaaaggcc acatggtatt ttgccgacga gttccatttg
    10801 cttttgaaag aggaacagac ggcggcctat tctgcggaga tttggaagcg gtttaggaaa
    10861 tggggcggca ttccaacagg tgccacccag aacgtcaaag acctgctttc ctccccggaa
    10921 attgaaaata tccttgaaaa cagcgacttc attacgctgc tcaatcaggc gtccggggat
    10981 agaaaaatcc ttgcggaacg cttgaatttg tccaccgaac agcagaagta catcgacaat
    11041 tccgaacccg gcgagggcct tttgatcttt gaaaacgtgg tgctgccctt taccaacccc
    11101 atcccgcaca acacccagct ttacaagatt atgacgaccc gcttaaacga ggtggcggga
    11161 gtatgacccg gcgacgccac aaaggaggtg aaccaccatg aaagagctga aagccaaggc
    11221 gaaagtgacg caggccatga cccgcgacgg gctgacgctg gaaaatcagg ccaccggcga
    11281 ggtggagaac atttccagcc gggaggccga acaggatttg tccgcgccgg ggccgggcgg
    11341 caccgccgat aagctgctgg aacgggccga cgccatccat gacggccata aggccaagaa
    11401 agccgcgaag caggccgggg ctgtggccgc cgagggggac gctgccctgc accggccatc
    11461 cgcccggctc caactgtcgg aggaagaacg ggccgacccg gatttacagc cctatatccg
    11521 aaaatcggac aaacgggccg ataagctgga taaggcccgc gccgccatcc ccaagaagcg
    11581 cgtcccggtc aaggaaaccg tctttgacga ggcaagcggc aaagcaaaga ccgtcctgca
    11641 ctttgacaaa caggacaagg ggcctccctc cctgaaaccc aatccggcag gccgtcctgt
    11701 ttccgaggtt tttctattcg gccacgggaa aatccatgaa gtcgagcatg aaaacgtggg
    11761 tgtggagggc ggccataagg gggaggaact tgccgagcgg caggccggaa aagccatccg
    11821 cagcaccatc cggcaccaca ggctgaaacc ctactgggac gcggaaaagg ccgagcgcag
    11881 gctattgtct gccaacgcgg agtatttcta ccagaaatct ttgcgggata acccggagat
    11941 cgcacaggcg gccagcaacc ccatttcccg gttctggcag aaacagcaga tcaagcgcca
    12001 gtacgccaag gccgcccgga cagcgggcca gaccgcaggc gcggcggcac agggcgcggc
    12061 caagacagcc ggggcctcca cgaaagcggc caaggccgca gccaaagaaa gtgaacgggc
    12121 ggcctccttt gtggcccgcc actggaaagg ggcgctggcc gtccttgtgt tggggctttt
    12181 gatgctgctt atcatcggcg gcttacagtc ctgcaccgcc atgttcggca gcgccggaag
    12241 cggcatcacg gcgtcctctt acttttccga ggatagcgat atgttggccg ccgaggacgc
    12301 ctacgcggag ctggaagccg agctgcaaga ctatctggac aactacgagg ccacccatga
    12361 ctatgacgaa taccattttg accttgacga gatcagccat gacccctatg tgctgatttc
    12421 cattctgtcg gcgctccatg acgggatatt taccatcggg gaggtacagg gtgatttgtc
    12481 catgctcttt gacaagcaat atatcctgac agaaaacgtc gtggtagaaa cccgttaccg
    12541 caccgaaacc gatacatgga cggacgcgga gggcaacacc catacagaca cctaccaagt
    12601 gccgtatgac tattacatct gctacgtcac gctggaaaac ttcgacctgt cccatgtgcc
    12661 agtctatatc atggacgagg aaacgctaag tttctattcc ctgtatatgt ccacgctagg
    12721 aaaccggcct gatctgtttt ccggcagcca gtaccccaac gcctccaccc tgacgccgcc
    12781 gacctactac gacgtacccc cggaggcttt ggaggatgcc acctttgccg ccatgctcaa
    12841 agaggcggaa aaatatatcg gttatcccta tgtgtggggc ggcagctctc ccagcaccag
    12901 ctttgattgc agcggcttca tttcgtgggt aatcaatcat agtggatgga acgtgggacg
    12961 gctgggggcg cagggcctat ataacatctg cacccctgtt tccagttcgc aggccaagcc
    13021 cggcgacctt gtatttttca agggaaccta cgacaccccc ggcgtgtccc acgtcggcct
    13081 gtatgtgggc aacaatgtga tgctgcattg cgggaacccg atttcctaca cgaaccttac
    13141 ttcaaactac tggcaatccc atttttacag ttacgggcgt ttaccttaac gccagaaagg
    13201 agctttcaat tatggcaacc aaacttgacc gcatcgaaaa ggacatccag aaaaccaagt
    13261 ctaagatcgc ggagtttcag aaacagcttc gggagctgga aacgcagaaa accgaacagg
    13321 agaatttgca gatcatccag cttgtgcgcg gcatgaacat gaagccggag gaatttgccg
    13381 cgttcttacg cagcggcgca atgcaggccg cccctgccgc aaccccttac catgaacagg
    13441 aggacaatgc ccatgaagaa taagaccaga cggatttccc gtattctccc cgcacttctg
    13501 gccgtgatgc tctgcgtcac ggcctttccc gttaccgccc ttgcaggcgg caatgacccg
    13561 gcaccggccc cgctgccgga ggctacaacc gaacccgcta cgggcggttt ggagcctgaa
    13621 acagacgaaa ccggcaccgc cacggacggg acagaaaccg aggccgaaaa agataccggc
    13681 cctctgaccg gcaaggacat caccgacctg ttttccgcgc tgttcggttc caaggtgagc
    13741 attgccgcca cggacgacgg catccagatc accaccggca ccgacaaaaa agaacctgaa
    13801 cagaccggca ccgtcaccac caacggcggc aggctgaatg tccgcaccgg cgcggggctt
    13861 gacaagaccg cgttcaccca gcttcccaat gggacgacgg ttgaggtagt cggcacagac
    13921 ggcgactgga ttaaaattct cttgccggag cgcatcggct atgtccattc cgactatatg
    13981 accgtcagcg aaaaggaagt agcggctacc ggcgagggcg gtttttccct ctccattgac
    14041 ccggaggaaa ttgcttctct gctggaactg ttcaatggcg gcggggccag cgccgccctg
    14101 tccccggacg ggaacctgtc actgattgac gacatcggca gcgccgcgaa aaatggcaag
    14161 cagtttatca ccgtggaaac caaaaacggc aatgtgtttt atctgattat tgaccgggac
    14221 gacgagggaa aagaaactgt ccatttctta aatcaggtgg atgaagccga tctgctgacg
    14281 ctgatggagg acggggaaac cgtcaccccc gctgctgtct gctcctgtac cacgaaatgc
    14341 aaggccgggg ctgtgaacac gaattgcccg gtatgtaaaa ccaacctgac cgagtgcagc
    14401 ggcccggagc cgcaggaacc ccagccggag gaacccgaag ccccggagga agaacccaag
    14461 ggcggcgcgg gcggcctgat cgtgttcctg ctggtggcgc tggcgggcgg cggtgcggcc
    14521 ctttactatt tcaagttcaa aaagcccaag gctgaaacca ccggcagcga cgatttgggc
    14581 gagtatgatt ttggggagga cgaggatttg gaggacgagg aacccgaaac cgagtttgac
    14641 ccggaagctg attttgtgag tgaacaggag gacgaggaat gacatataaa cttgtaatcg
    14701 cggaaaaacc cagcgtggcc cgcagcattg cgggcgtgat cggggccacg gagaaacacg
    14761 acggctattt ggaggggaac ggctatctgg tgagctggtg catcggacat ctggtgtcct
    14821 ttgccgacgc cggacgctat gacgagcgtt tcaaaaaatg gcgctatgag gatttgccca
    14881 ttctcccgga aacatggcag tacatcgtcc ccgacgacaa gaaacagcag tttgacgttc
    14941 tctgttcgct gatgcagcgc cccgacgtga ccggccttgt gtgcgccacc gacgccgggc
    15001 gcgaggggga attgattttc cgtttcgtct accagatggc gggatgccaa aagccctttg
    15061 agcgcctttg gatttcctct atggaggaca gtgctattaa ggacggcttt gcccacctga
    15121 aaccgggcgg cgactatgat ccgctgtatc aatcggccct gtgccgggcc aaggccgact
    15181 ggctgatcgg cattaacgcc acccggcttt tttccgtcct gtaccacaag aacctgactg
    15241 tgggccgcgt ccagacgccg accttaaaaa tgctggtgga cagggacgcc aagattaccg
    15301 atttcaagaa agagaaatat cacatcgtcc atatcacggc gggcggcgcg gacgcggtaa
    15361 gcagccgctt ttccgacgct gccgaggcaa acaccgtcaa ggcggcttgt gcaaaagcgc
    15421 aggccgtttg cgtttccgtg acgcgggaga aaaagacgga acagccgccc aagctctatg
    15481 accttaccac cttgcagcgg gaggctaacc ggcttttcgg ctataccgcc aagcagaccc
    15541 ttgactatgc ccaggcactg tatctaaaga agctgctgac ctatccgaga acggatagtc
    15601 aatatcttac cgacgatatg ctgcccgccg tggaaagcct tgtgtccggc ctgtgggagc
    15661 tggtgccgtt tgccaagggc ctgaaccttt ccccgcagtt tgaccgcatc ctgaacagca
    15721 aaaaggtgtc cgaccaccac gccattatcc ccaccgctga atttgtgaag cagggctttg
    15781 gcactctggc cgagagtgaa aaaaagctgc tgtccctgat ctgctgcaag ctcttatgtg
    15841 ccgtggccgg gccgcatatc tatgaggccg tcaccgccac atttacctgt gccgggcagg
    15901 agtttaccgc caagggcaag accgtccttt cccccggctg gaaagagatt gaacgccggt
    15961 tccgttcttc cttaaagacc gacgccgacg aggacgccga ggcggtgcgg gagctgccgg
    16021 agctttgcga gggccagacc tttgcagacg tggccgcctc tgtcaccgag catttcacca
    16081 cgccgcccaa gccctacacc gaagatacgc ttttgtccgc tatggaacgg gccggggccg
    16141 aggatatgcc ggaggatgcc gagcgcaagg gattaggcac accggccacc cgcgccgcca
    16201 ttctggaaaa gctggtgcag atgggctttg tccagagaaa aggcaagcag ctcattccca
    16261 ccaaggacgg catcaatctg gctgtggtgc tgccggaggc gcttacttct ccccagctta
    16321 ctgccgaatg ggaaaaccgg ctgaccgaga tcgccaaggg gcaggccgat cctgacgaat
    16381 ttatggacgg catcgagacg caggcgcggg agctggtgca gacctattcc tgtatcagtg
    16441 aggaaaagca gaaattgttt caggccgagc gtgttcctat cgggacgtgt ccccgctgtg
    16501 gtgaggctgt ctatgagggc aagaaaaact actactgcgg aaaccgggcc tgccagttcg
    16561 tcatgtggaa gaatgaccga ttttttgagg aacgcaagaa agcgttcacc ccgaagatcg
    16621 ccgctgcgct gctcaaaacc ggcaaagtca aggtaaaggg cctctattcc gtaaagaccg
    16681 gcaaaaccta cgacgggacg gtgcttttgg ctgataccgg gggcaagtac gtcaactacc
    16741 gcattgaaca caggagctaa aggacagtaa cacagcaagc atagggagtg tggctacact
    16801 cccggtaaaa tcccccggcc gcgtccctgc cgggccgggg atttttgctt gttggggaag
    16861 tccccaaccc cctatttttt gaaacagaac agagaaagga gtgaccgcgt gaacgaagta
    16921 ttttctatcc aagtccataa ccggcagttt ttggaacagg gcaaacaagg cgtctggctg
    16981 gatttgccca ccacaaccga aacgctgcaa gccgccctgc gggatgttgg cattactgcc
    17041 gacaatccgc aggatttttt tatcaacggg tattcctgcc cggaggacag acaccttgcc
    17101 attccctatg acatggtgct ggccgccgac gtggacgagc tgaatttcct tgccgcccgg
    17161 ctgggccagc ttgacgccgc cgagatcgca gagctgaacg ccgccctgca aaatccccgt
    17221 ggcggctttg agaatatcgg ccagatcatc gactacaccg aaaatgtgga ttactatgtg
    17281 catcttcccg acgttagggg ggatggacag ttgggcgact actacctgaa ccgttccggc
    17341 atggtggata tgccggagga atggaaagcc gggattgata cggcgcagtt tggacgccac
    17401 attgcccagc aggaacaagg cgtttttacc cagtacggct atctggtgag aagcggcgac
    17461 gaatggcagc gcgtccatga gggccagccg gtgccggagg aataccgggt gatgcactac
    17521 ccccagccgg aagctgaccg ggaggaaccg caggcccagc agccggaggc atccgaaagc
    17581 cccgacaaag cgccgccccc ggttatcccg atcatgctgg acggcatgaa cagcaaagaa
    17641 cgcatgaaag tgattactga ccgtctggaa accggcgtac aggagctttt tgaaagtagc
    17701 cgctatatgg cttacttatc cgctatgtcc aagatgcacg attacagtct gaacaatacg
    17761 ctgctgatcg ccatgcaggg cggttcgatt gtggcgggct ataacaggtg gcaggatgaa
    17821 tttgaccgcc atgtgaagcg gggcgagaaa ggcattaaga tactggcccc ggccccgttc
    17881 aaggttaagc agcagatgga gaaaaccgac ccggccaccc agcagcccgt gatcggcgcg
    17941 gacggcaagc ccgtgaccga agaacaggaa gtgacggttc cggcctttaa ggtggtgaca
    18001 gtctttgaca tcagccagac cgagggcaag gattttccca tgccctatat cagtgaactt
    18061 accggcgacg tgcagcagta tcaggatttc tttgccgcgc tggaacggac atcaccctat
    18121 cccataagca ttgagccgat ttcagacggg gccaagggcc gctgcttcta cgcaaaacag
    18181 cgcattgcca tcaacgaggg catgagcgaa ttgcagaccc taaagaccgc catccatgag
    18241 attgcccatg ccaagctcca cgccattgac cgggaggccc ccgccgagga acaggcggcc
    18301 cggccagacc gcagcacccg cgaggtacag gccgaaagcg tggcctatac cgtctgccag
    18361 tattacgggc tggacacgtc ggactattct tttggctatg tggccgattg gagcagcggc
    18421 agggagttat ccgagctgaa agcctccctt gaaaccatcc gggccacggc cagcgaattg
    18481 ataaccgaga tcgacggcca ttttgcccag cttcagcagg agcgcgaggc ccggcaggaa
    18541 caggaggccg tcacggttga cgttaccccg gaggcagcgg ccccggcgca ggccgaggaa
    18601 gaacaggccc cggcccttga tccggcagcc gagccgcttg tgactgtgct atggagcgaa
    18661 agcccccagc taaaggacgg ccagcaaatg ccgttgcatg aggccgacgc gctgtttgcc
    18721 cgtctggatg cagcatatcc ggcagaggca ggctatgaca aaaccgcgtt ccgcattgac
    18781 tgtatctttc agggcgagcc ggacagctac gaggggcggc aggattttgg agatagggac
    18841 ggttccctga tcgaacatat ccagagctac caccaatttt acgcgcagga cgaaaactgg
    18901 aaaaagcatg taatcgctac aaccggcata gagggctggg agcaggacaa ggcccatcgg
    18961 gatatgctgt taggcgagtt tgtcccctat atgaagctgc attgcagcct tgcccaaatg
    19021 gaacagacgg cgcagggccg cttacagtcc ggggaaaccc tgacgccgga ggaaacggcc
    19081 tatttggggg cgcttctctc ttatgtgcag gagtgccgcc ccatgctcaa tcagggccag
    19141 tatcagttgc cggagccgcc gaaactggct gacttcgacc agagcttgca ggactacaaa
    19201 gcacaggtac aggccgagat cgcgcaggag gccgccgcag ccggtatgac cgtggaggaa
    19261 tacgcggcgg ctggctatga agcaccccaa caggacacct tttccatcta ccagcttcga
    19321 ggcggggagg aaacccgcga ctatcggttt gagccatacg agcgtttaca ggccgcaggg
    19381 ctggccgtgg acagtgccaa ctatgacctt gtttatacgg caccgctgga taaaagcacg
    19441 tctttggagg atatttaccg cacattcaac atcgaccacc cggcagattt taggggacac
    19501 agcctttccg tttccgacgt ggtggtgctg caccagaatg gacaggacac cgcccactat
    19561 gtagacagct tcggctatat ggaagtgccg gagtttttgc gggaaaatcc cctgcgtacc
    19621 gccgaactgt ctacggagca gaacgaaaac atgattgacg gcattatcaa caatacgccg
    19681 cccgaaccgc agccggagga acccgaacag ctatcttttg aggatgtggc agttgcttcc
    19741 ggccagacac aggaggaacc cccggaggct gcgcccccgc aaacggctgt ccgctattat
    19801 cccatcaatg agggagccgc ccgccgcgcc aaggaagcca tgagcttttc tgactaccgg
    19861 cccggcagcg ccacggcgga ataccggcac tatgtcgatc aggccgtgga aattgcggag
    19921 cgccagaaaa agcgtgtgga gccagagtat cacgaaaaga tagacagcct ccttgacacc
    19981 tacgcccgca agctggccga gaacatgaat aagggctatg agattaccgc ccgcgtcccc
    20041 tctgtcctga ttgccgggcc gtccaatttc ccggtacggg cgaaagaaaa gcagaacgcc
    20101 gccagtgacc gcaacatgga ggaatttcag tacattcagg gcttgcttga caaaatccgc
    20161 agcaccggca tgggtggtat cagcgccgac gatccgcagg ccgtttccaa gctggaaaag
    20221 aaactggaaa agctggaagc gtcgcaggag ttgatgaagg ccgtcaatgc ctactaccgc
    20281 aagcatggga cgctggacgg atgcccccat ctgatcgaaa gaggcattga aaacctgaaa
    20341 gcggatatgg cgtcgggctg gcactatgag aaaaaaccgt tccagtcctg gcagctctcc
    20401 aacaataacg ccgagatacg ccggttaaag ggccgcattg aggaattgac ccgccaaaag
    20461 gaggcggcct atgtgggatg ggagtttgac ggcggcactg tggagatcaa ccgggtggcc
    20521 aaccgcctgc aaatcttctt tgaggggaag ccggatgctg ctgtacgcga cgagctgaaa
    20581 agcaacggtt tccgctggtc gcccaaggcc ggggcatggc agcggcagtt aaacgacacc
    20641 acaatccgcg tcgctgaccg tatcaagtgc atccagcccc tttccggcga aaagcccagc
    20701 gccttacagc ttgcggcccg cagggagaaa gaaaagccct ctatccgggc gcagctcaac
    20761 gcggccaaga ccgaacaggc caagcagcca ccggcgcggg aaaaatcaaa gggattggag
    20821 gtagagtaaa tgcagaagtt tgaaaatgtg gacgttctgg ccgcgctgga acagatcatg
    20881 cggcagaaca ccgcgtttta tcaaagtgac tttggcattg acaaagacat tatcaggcgg
    20941 gatgccgcca gcgatcaggc cgcagacaag accctgctct ggatgtcccg gccctccggg
    21001 acgtactgtt tccgggaacg tgatgttttc ctgaaagaca cccgacagta caacacatgg
    21061 aaattttacg gggagcagac ccgtgacaag atactggcct acgcggtgga gctgaccggc
    21121 acacagggcg gcactatccg gggcaatctc tatgagctgg actatccgca gcatttccgg
    21181 cacgtcattg aaaccgccca gccggtgagt gtgaaccggc tcttttatga gcatggcacc
    21241 cgtgacatcc cggagagcca gcgttttgac ggaagccctg accgggtatt gggaaacttt
    21301 ttgtactatg aggcccagcc ccatgacccg gctgtgctgc aggaggcgct acggacggag
    21361 cagcacagcc gggggcgggc cgtccccggc gatttcaaag tccacgtcac cgctttgcat
    21421 gacagccgga ttgaaaccga ggcccggcgt attgtggaca gcctgaaaga tctgtccgcg
    21481 cctaacagcc ccaataaaac gcatttcatg gtggagatct ccccgcactt tgcggcgatt
    21541 gcttccacca aggataatga ccggctcttt gccatgctgc cctataaatc cctgtgtttt
    21601 accgggatga aaggcaggca tggcgtgtat gccgtgatta accgggacga gcgccgggat
    21661 gtgagtattc gcaggccccg gccctctatc cgcaagcagc tatccgacag caaacaggcc
    21721 gccagcccaa aaaaggcggc ggccagaacc aaaaagaatg aattggaggt ataaggaatg
    21781 atgcacttta ccgtggagga agaaaacctg atctgtatgt accacaatgc agaccggcgc
    21841 agaacgataa acaggctcac cgccgtcatg ccggttatgg atggggatat gcgggcgcag
    21901 gcccagcaga ccctttccaa gctggaacgc atgacggatg ccgactatga cggccagaag
    21961 ttccacttca cagacgaata acgaacagca gacgccgggc gcggcggcca tgtggtcgct
    22021 tgcgtccggc gtcttttctt tttgcctatt cctacgcatt tagaacccgg tattgtcggg
    22081 gataggatga agtattcgct tgcccctgca aacatgcggg agaagccttg cagcgggcgg
    22141 ttttcaatcc cttaaatgcg taggttgcag ctataattag acccctgaaa cagatatagc
    22201 cgcgcagcgg gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga
    22261 gggtgcagcc ctcttgccgt ctatctctgt tccagcggga agctcggcaa tgcttccagc
    22321 agtttcaggg aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt tttgccgttt
    22381 gcctgtctga tctccaccgt ggaaagctcg ttgatgtagt cagcataatg ggccaacact
    22441 ttttccgtgg cccacctctc accggcaacg gcggctttga tcgtttcata gtccagcagc
    22501 ttttcttgtc cgtccatcct gatacccccg tcagactgat tttttctatg ataccatatt
    22561 cggaagcatg gaaccagaga aaacggcgct tttctgctgg taaattctac gcatttagaa
    22621 cagcacttcg gcggggatag gataaaacac ccacataccc cgctgaccat gccggaaaag
    22681 ccttgaaata taagggcttt tacagctcta agtgcgtagg tttccagtat cttttccgct
    22741 ggcgctcggc ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag tatttggccc
    22801 ggttagactt cggcgtgaac gtggccccgc actcacagca gcgtttcact tcgtcccggt
    22861 ctttggtgat ctcggcatac agggccgcat ccagcgggag gacagcggcc ttaaaccagc
    22921 ggcacagaag cgaataggaa atgctctgta cgcaaataca ttcttcgccg tcgtccagca
    22981 acaggcagtt gccgtcacaa tagttgcagc actcatgcgt cagcttccgg gccttgcgat
    23041 attgagggta attcattcgt ggaatactca tagctcttgc gtccattcct ttcccggcgt
    23101 catacgcaaa atgccgtcca cattctgctt gatgatgccg tattcctgca tctgcttttt
    23161 cgcttcgccg tacttctcat acagccgttc cttttcctcg gccaatcgct tgtactcggc
    23221 tttcagggcg tacagatcgg ggagcttttt cacaccctgt ccctttaggg cctttgccgc
    23281 agcttcatac aaaatcaacg tgctttcatg ctcacgccgg aattttgcct tatccgctgc
    23341 attgcggtat tccatagcca cgggccagag ctgcttgtag gtagaaatat tctttatcag
    23401 caccgccata tccgcaagcc ggtgttccat agctttgagg gtagcggcgg cctcgtcgct
    23461 ggcggcggtg atctcggcca ctttgctttc caaatccggg taggtgtcta tcccatgttc
    23521 agtcaggaaa ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg cccacctctc
    23581 atagcctgcc gactgctggg ccttaatgtt gttttccaga tcaatccgca aagaaatccc
    23641 cttgcgctct ttgggcgctc tggtgataac gcggcctttg atacgctctt tgatggcttc
    23701 ctcggtgtag gcttccccca atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg
    23761 gaaagaaata aactttcccg gcttgatctc atagccctgc gcctgcatga gccgcaggaa
    23821 atcatcaaaa tcttttgcct gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt
    23881 ccagctcgtc cctttccgct gggcgtccca ctcggcatag ctcttgccct tactctggcc
    23941 gggcttcacc acggacaatc cgttttcctt gcacagcctg tcgctggtgc gccggatata
    24001 ggcatagctc tgtttgttgg aaatgtactt gcgctgattt accatatcca cggcacagaa
    24061 aataatgtga ttgtggacgt ggcccttatc aatatgggta gtcagcacat agggatattt
    24121 cccttgcagc acttcctcgg caagctgctt cccgatctca tgggcctgtt ccggcgtggc
    24181 ttcgccgggg gcaaaggatt gtatcaggtg gtggcccaga ttattgccct tttggtatgc
    24241 ctgcgccagc agcatttcaa attcaatgtc cgctgtttca taagagcagg caaaggaggc
    24301 caccagcagc ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg cttttttcag
    24361 cgttgacttg ataggatgta tctttgtaac tgccatatct gcgccagcgc cccctttata
    24421 tcctcgatgt cctgtgcgta caccggcccc ataccgttca gcctctttgc aatctgattg
    24481 acgttgacgc cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg
    24541 ttgacgatat agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc
    24601 agcagcgcca ttttctgttc aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc
    24661 acgatttttc gctttctgtt tgccatgctt caccgtcctt tccattcagg ggtttgggga
    24721 tttccccaac aagcaaaacg gacgcggccc ggcagggacg gggcaggccg tttttccggg
    24781 agtggctaca ctccctatgc ttgctacgtt actgtttctc cactaacaag acgtttccaa
    24841 agccccaaac tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc
    24901 tacgctgtgc agctcccgct aaaagtaaaa cagaggatgc cagttacaca tcctccgttt
    24961 cattcttctt cggcttttca attagtatgg cgctgtcaat cgcgccctcg atgatcggca
    25021 ggtattcttc ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct
    25081 cccgcatgat ctccgggtta aagtagcgtt ccacaggaag cccgcaggct ttaataagct
    25141 gtatgacaac cggcagactg ggaatggccc gtcgttttcg atgttggcaa gatagcgcca
    25201 gtcaatattt attttctctg ccattgcttt gcgcgaaacg cgctttgctt gccgggccgc
    25261 tttcacatcc gcgccgaaag tttcaaagcc gggacaatct tcaatattcg ccatatcgca
    25321 cctcccactt acattgtata tttcatactc tgcccgtgga atgttggtat atgcaccttt
    25381 cttaaagttt atatttcaca ttttgtttct tttattcttc gagcaaaaga attataatgt
    25441 accctgtgga ggtgtactat ggactacatg acgttgaaag aggcaggcga gaaatggggt
    25501 gtgcccccac gatggataaa ctactattgt gctgcgggac gcatccccgg cgctgtaaag
    25561 atggcgacga tctggctcat tcccaaaact gcggaaaagc cgattgacag gcgtacaaag
    25621 aaaggaaagg cggttactga atagtataat gaaaccggcg cattttggat atggttttga
    25681 agagagagaa agcatggtat tttgtcacgg aaaaactata agatgataga tggcaggctg
    25741 ctccagaccg ataaaaagta ttctcatctg aaacagaaag aaaagattta cacatggatg
    25801 tatgaagaag cgaaacttta tcatgacaga aatggaaaat gcccgaaaca aagacgagga
    25861 agctgctgct gtatcagccg tatatggtag aattgaaaag gccggcatct ggattcttta
    25921 tggtgaagtc tccagacatt ggataactgc tggtctaatg cgcctttgag tatggggtga
    25981 ttgattttaa taccggggag gtacggcttt ttgaggaaaa tattctggga tttacatttg
    26041 gcgattatta catttcctgt aactgcaagg acacggatga tgaagatatg aggaatatag
    26101 acttgctgat tttctactgt cttgaacggt attaggactg aatctgctgc gcggagtgcg
    26161 gcaagagcga ccggagggag tacacacagg ccctgcctgt gtgaaaagcc ataccgcaag
    26221 gtgcattcgc aaaaaagagg ccgcgcattt tgctgaggct tggggtgtcc ccaacaagca
    26281 aaacgagaac gccgcaggcg ggcaagtggt ggatcagaat atccgtaaag gatattctga
    26341 tccattgctt gccagtatta tcgggatgtt ttgacaaccg ctgtaatcga aacatcccaa
    26401 acattaagaa ggtgttaatt attattatct tgatttaatt cgtttaaacc aataatatag
    26461 atacatacac catgaaaaga agcgagtatc ccaatgacgg atgattaaaa tccgaaaaga
    26521 aatcgctttg aaactacagg gaaactacag actgttatgt tatcttctta aaatggaggg
    26581 atttttatgt cgatacgaat tctacttgtc gaggatgatg atcatatctg taatacagta
    26641 agggcgtttc tggctgaggc aggatatcag gtggatgcct gcacagatgg aaatgaggca
    26701 tacaccaagt tttacgaaaa cacttatcaa ctggttattc ttgatattat gctgcccggt
    26761 atgaacgggc atgaactttt gcgtgaattt cgtgcgaaaa atgatactcc cattctgatg
    26821 atgacagccc tgtcggatga cgaaaaccaa atccgggcgt ttgatgcaga ggcagacgac
    26881 tatgtaacaa agccattcaa gatgcagatt ttactaaagc gggtggaagc cctgctgcgg
    26941 cgcagcggtg cgctggcaaa ggaaatccgt gtcggcaggc tgacacttct gccggaggat
    27001 ttcacggtac tttgtgacgg tacagagctg cccctgacac gaaaagaatt tgaaatcctt
    27061 ttgctgctgg tgcagaacaa aggcagaacc ttaacgcatg aaatcatttt gtcacgtata
    27121 tggggatatg actttgaggg tgatggcagc acagtccaca ctcatatcaa aaatttgcgg
    27181 gccaagctgc cggaaaatat catcaaaacc attcgcggtg taggataccg attggaggaa
    27241 tcataataat ggaaagaaaa gggattttca ttaaggtttt ttcctatacg atcattgtcc
    27301 tgctactgct tgtcggtgta acagcaacac tgtttgcaca gcaatttgtg tcttatttca
    27361 gagtgatgga attacagcaa acagtaaaat cctatcagcc attggtggaa ctgattcaga
    27421 atagcgatag gcttgatatt caagaggtgg cagggctgtt tcactacaat aaccaatcct
    27481 ttgagtttta tattgaagat aaagagggaa gcgtactcta tgccacacca aatgccaata
    27541 catcaaatag ttttagaccc aactttcttt atgtggtaca tagagatgat aatatttcga
    27601 ttgttgctca aagcaaggca ggtgtgggat tgctttatca agggctgaca attcggggaa
    27661 ttgttatgat tgcgataatg gttgtattca gccttttatg cgcgtatatc tttgcgcggc
    27721 aaatgacaac gccgatcaaa gccttagcgg acagtgcgaa taaaatggca aacctgaaag
    27781 atgtaccgcc gccgctggag cgaaaggatg agcttggcgc actggctcat gatatgcatt
    27841 ccatgtatgt caggctgaaa gaaaccatcg caaggctgga ggatgaaatc gcaagggaac
    27901 atgagttgga ggaaacacag cgatatttct ttgcggcagc ttctcatgag ctaaaaacgc
    27961 ccatcgcggc tacaagcgtt ctgttggagg gaatgcttga aaatatcggt gactacaaag
    28021 atcattctaa gtatctgcgc gaatgcatca aaatgatgga taggcagggc aaaatcattt
    28081 ccgaaatact ggagcttgtc agcctgaatg atgggagaat cgtacccata gctgaaccgt
    28141 tggacatagg gcgcacggtt gccgagttgc tgcccgattt tcaaaccttg gcagaggcaa
    28201 acaaccagcg gttcgtcaca gatattccag ccgggcaaat tgtcctgtcc gatccgaggc
    28261 tgctccaaaa ggcactatcc aatgtcatat tgaatgcagt tcagaacacg ccgcagggag
    28321 gcgaggtacg gatatggagt gagcctggtg ctgaaaaatg ccgccttttt gttttgaaca
    28381 tgggcgttca cattgatgat actgcgcttc caaggctgtt caccccattc tatcgcattg
    28441 atcaggcgcg aagcagaaaa agtgggcgaa gcagaaaaag tgggcgaagc ggtttaggac
    28501 ttgccatcgt acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg gaaaacacct
    28561 cggatggcgt tttgttctgg ctggatttac cgctcacatc aacattgtaa atatttaaaa
    28621 cttaaatgat ttt
//
LOCUS       DAYYHS010000029        27927 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000029 DAYYHS010000000
VERSION     DAYYHS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.029"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(239..1183)
                     /locus_tag="ACHA6A_001263"
     CDS             complement(239..1183)
                     /locus_tag="ACHA6A_001263"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8969473.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1479..2423)
                     /locus_tag="ACHA6A_001264"
     CDS             complement(1479..2423)
                     /locus_tag="ACHA6A_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8969474.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2644..3858
                     /locus_tag="ACHA6A_001265"
     CDS             2644..3858
                     /locus_tag="ACHA6A_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8969475.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3880..5895
                     /locus_tag="ACHA6A_001266"
     CDS             3880..5895
                     /locus_tag="ACHA6A_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8969476.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5978..7351)
                     /locus_tag="ACHA6A_001267"
     CDS             complement(5978..7351)
                     /locus_tag="ACHA6A_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8969477.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7377..7661)
                     /locus_tag="ACHA6A_001268"
     CDS             complement(7377..7661)
                     /locus_tag="ACHA6A_001268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8969478.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7663..9678)
                     /locus_tag="ACHA6A_001269"
     CDS             complement(7663..9678)
                     /locus_tag="ACHA6A_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8969479.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9857..10162)
                     /locus_tag="ACHA6A_001270"
     CDS             complement(9857..10162)
                     /locus_tag="ACHA6A_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8969480.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10251..11282)
                     /locus_tag="ACHA6A_001271"
     CDS             complement(10251..11282)
                     /locus_tag="ACHA6A_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8969481.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11489..12364
                     /locus_tag="ACHA6A_001272"
     CDS             11489..12364
                     /locus_tag="ACHA6A_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8969482.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12516..13358)
                     /locus_tag="ACHA6A_001273"
     CDS             complement(12516..13358)
                     /locus_tag="ACHA6A_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8969483.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13450..15327)
                     /locus_tag="ACHA6A_001274"
     CDS             complement(13450..15327)
                     /locus_tag="ACHA6A_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8969484.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15324..16745)
                     /locus_tag="ACHA6A_001275"
     CDS             complement(15324..16745)
                     /locus_tag="ACHA6A_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8969485.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16748..20137)
                     /locus_tag="ACHA6A_001276"
     CDS             complement(16748..20137)
                     /locus_tag="ACHA6A_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8969486.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20394..21785
                     /locus_tag="ACHA6A_001277"
     CDS             20394..21785
                     /locus_tag="ACHA6A_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969487.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21858..22505)
                     /locus_tag="ACHA6A_001278"
     CDS             complement(21858..22505)
                     /locus_tag="ACHA6A_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8969488.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22513..23199)
                     /locus_tag="ACHA6A_001279"
     CDS             complement(22513..23199)
                     /locus_tag="ACHA6A_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969489.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23205..24278)
                     /locus_tag="ACHA6A_001280"
     CDS             complement(23205..24278)
                     /locus_tag="ACHA6A_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969490.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24500..25174
                     /locus_tag="ACHA6A_001281"
     CDS             24500..25174
                     /locus_tag="ACHA6A_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8969491.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25179..26519
                     /locus_tag="ACHA6A_001282"
     CDS             25179..26519
                     /locus_tag="ACHA6A_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8969492.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26668..27654)
                     /locus_tag="ACHA6A_001283"
     CDS             complement(26668..27654)
                     /locus_tag="ACHA6A_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8969493.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8155 a         5265 c         5056 g         9451 t
ORIGIN      
        1 tgtatttgcc gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc
       61 gcttattgta atcaacagat taaatttcat atgttcatat gtacggatac attttcggac
      121 cgattaattt taaccattct atttatttgc ttacctatta gatatcaata aaaaactgct
      181 taacagctat ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt
      241 ataatacagt gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt
      301 cttcttcgtt gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg
      361 gcacacctgt aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca
      421 atacagcttg ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac
      481 catagtaagt tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag
      541 caatcacatc taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg
      601 gtttaccacc gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta
      661 agatatagcc gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga
      721 aacgagttgt atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt
      781 cgttccaagc gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt
      841 cgaatcctga tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa
      901 ctaaatctaa acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat
      961 cagcatcttt acattcttca tagccacctg accatacagc cacattttca tcgccccaag
     1021 ccataccatc taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca
     1081 atactaattc attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa
     1141 aacctgtacc gacgattact actttacgac ttgttttttt catgaataaa cagctccttt
     1201 tcataactat ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg
     1261 ttaattataa tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac
     1321 agataaatga caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact
     1381 gaaaataaat ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa
     1441 caattttcct tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca
     1501 ttgggagtac atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat
     1561 ccgatacgat tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca
     1621 aagtcgaatt ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat
     1681 ttattactaa gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa
     1741 gaagtaaaat gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca
     1801 tctgctgatg aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat
     1861 gtatagagat ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata
     1921 ttttcaatca aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta
     1981 tgttcccctt tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt
     2041 gccaaatgat tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa
     2101 atatcctctt gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg
     2161 tcactttctt ccatctctat cctttctcca ttaataattt ttaaacatgt accgctatac
     2221 atataattga tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt
     2281 ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc
     2341 tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt
     2401 tcgattgatt cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc
     2461 tcctttttgt aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa
     2521 agctattttt ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg
     2581 ctattgagag aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg
     2641 aacatgctta tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg
     2701 acttctgctg gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt
     2761 tctttatctg ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt
     2821 ttgattagta gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta
     2881 gtgattgcat cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta
     2941 gcttcaagag ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg
     3001 attgctgcct tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt
     3061 caaggatttg gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg
     3121 caatatacct ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt
     3181 gttcctacac ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta
     3241 atcaaaacga atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta
     3301 tataatgctg tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa
     3361 actaatgcag cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt
     3421 tttggctggc ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg
     3481 ggaatcggct ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg
     3541 atcctaacag gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc
     3601 caagcgccca agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta
     3661 ggtactttca ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa
     3721 acacttgctg ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt
     3781 ctagtagtac acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa
     3841 caattgacaa caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa
     3901 gatagatggc tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc
     3961 caacgggaag aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg
     4021 aacgtcaagt ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc
     4081 caaggagttg ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact
     4141 tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg
     4201 atgtttatcg agggagacga agtaaaatca gatattggct ggacagcaag taattttatt
     4261 gaagaatatc cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa
     4321 atctattatc aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc
     4381 ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat
     4441 atcgaatcta tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat
     4501 tgctactata agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc
     4561 tatttgttta taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt
     4621 ttatctcttt cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg
     4681 tcagtatcag aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa
     4741 cgagatcgct ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt
     4801 ttttttgacg aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc
     4861 aagcagcata tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac
     4921 cactatatga tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca
     4981 ttaatgagat acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa
     5041 gattggatat ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa
     5101 gtcttattac tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca
     5161 tatggagaga acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa
     5221 caaaacctat ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa
     5281 tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct
     5341 aacaaagacc aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag
     5401 attactactc catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg
     5461 aatatcgtct accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta
     5521 actttctggg aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga
     5581 gatccttatg gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga
     5641 cgctatttta tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct
     5701 aatctagatt catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg
     5761 cttgaaaaaa caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa
     5821 atgaatggta aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca
     5881 aaaataaatg cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt
     5941 ctgatattgt ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa
     6001 tgtttgttct ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga
     6061 tgattccaac tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg
     6121 gtactagata atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg
     6181 gaaggataaa taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg
     6241 cccctcttct accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat
     6301 gtcctactac cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata
     6361 gtgttcctac aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa
     6421 aaacgatagg aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt
     6481 ggaatgctgg aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc
     6541 cagctgtgaa tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta
     6601 gttctctttc aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca
     6661 tcaagagcat gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta
     6721 tgtcctctgt agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat
     6781 aaccgatatt tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa
     6841 acggttgaca aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata
     6901 agatcatcca caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag
     6961 aaacataaag cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa
     7021 gtaaaatatt cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt
     7081 gcaaagcaat cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg
     7141 tttggaacat cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca
     7201 gtactaggaa tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct
     7261 tctgaagaac gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga
     7321 ttgacacaaa caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat
     7381 tcttttgaca agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc
     7441 aaaggtacga gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg
     7501 aagaaataat cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac
     7561 tcgtccacat ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa
     7621 cttgttccta atccactttg acatactgca aaaattttca tatcattcgt ctccttttct
     7681 tatgatcgct aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt
     7741 attagaaagt agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc
     7801 agctaaacag atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg
     7861 ttgaagtttc agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg
     7921 taatgcgatg tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat
     7981 agctttgata taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt
     8041 gattgcttct tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa
     8101 ttcagatagc ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt
     8161 taatacttga tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag
     8221 cgccgacaac aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg
     8281 tacttgattg atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag
     8341 agactttttc ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt
     8401 ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa
     8461 taattgctga agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat
     8521 cacggctctt atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa
     8581 tagtatagtg aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa
     8641 cggaagtaat gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc
     8701 gatcatcaca ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg
     8761 ataaaagaca ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc
     8821 catgcgatgg atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac
     8881 atctccttca acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg
     8941 ttcggaatag ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc
     9001 caacagttca gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa
     9061 actcaaataa taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga
     9121 aatcgtttta tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt
     9181 taaatacgtg agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt
     9241 tgcttctctt cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata
     9301 cgacagctga caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac
     9361 tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt
     9421 cgaataaacc atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa
     9481 tcgagtaata tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat
     9541 acgggtatca tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt
     9601 tacttcgatg atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata
     9661 actaattttg tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct
     9721 tttaaatcga ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa
     9781 cgatttctat cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg
     9841 aattgatttc gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt
     9901 tttctttgac atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag
     9961 gctttgtttc ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt
    10021 caccagagtt tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc
    10081 ctgctaaagc cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt
    10141 tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta
    10201 caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt
    10261 ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt
    10321 tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag
    10381 acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca
    10441 tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca
    10501 ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc
    10561 cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt
    10621 atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct
    10681 ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga
    10741 acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca
    10801 tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata
    10861 tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt
    10921 ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct
    10981 gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt
    11041 gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc
    11101 tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc
    11161 cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt
    11221 gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc
    11281 atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat
    11341 ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg
    11401 aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat
    11461 cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga
    11521 gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc
    11581 agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat
    11641 tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg
    11701 ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc
    11761 aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat
    11821 aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa
    11881 caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt
    11941 ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc
    12001 ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg
    12061 tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt
    12121 gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat
    12181 atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga
    12241 ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg
    12301 caacttgaaa aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac
    12361 ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata
    12421 tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac
    12481 gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac
    12541 gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca
    12601 tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca
    12661 tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta
    12721 caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc
    12781 ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata
    12841 tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt
    12901 cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct
    12961 tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa
    13021 tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta
    13081 tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct
    13141 tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca
    13201 ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc
    13261 agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc
    13321 atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact
    13381 gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt
    13441 gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag
    13501 aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt
    13561 acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga
    13621 ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt
    13681 cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact
    13741 tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga
    13801 tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc
    13861 tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc
    13921 agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc
    13981 ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa
    14041 tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa
    14101 tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata
    14161 taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt
    14221 atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa
    14281 tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt
    14341 caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg
    14401 tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc
    14461 attatcagga gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat
    14521 caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg
    14581 accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt
    14641 gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc
    14701 tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc
    14761 attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt
    14821 ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc
    14881 ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt
    14941 ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac
    15001 tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt
    15061 atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa
    15121 tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg
    15181 taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac
    15241 tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat
    15301 aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac
    15361 cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg
    15421 tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg
    15481 tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt
    15541 aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg
    15601 gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt
    15661 gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg
    15721 aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta
    15781 ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg
    15841 ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg
    15901 gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg
    15961 tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt
    16021 aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga
    16081 tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc
    16141 gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt
    16201 tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca
    16261 ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca
    16321 atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg
    16381 ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac
    16441 gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga
    16501 tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac
    16561 gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt
    16621 gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc
    16681 tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt
    16741 ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac
    16801 aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg
    16861 gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca
    16921 gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag
    16981 taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag
    17041 ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt
    17101 ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt
    17161 cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc
    17221 atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga
    17281 tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga
    17341 ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt
    17401 ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc
    17461 ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa
    17521 cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt
    17581 ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc
    17641 ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca
    17701 atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa
    17761 tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat
    17821 ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat
    17881 ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg
    17941 cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat
    18001 ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt
    18061 taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa
    18121 tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact
    18181 ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt
    18241 cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg
    18301 gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt
    18361 cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag
    18421 tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga
    18481 atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag
    18541 cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat
    18601 cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga
    18661 gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt
    18721 ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata
    18781 gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat
    18841 cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct
    18901 gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc
    18961 catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac
    19021 catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca
    19081 tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat
    19141 tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct
    19201 ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga
    19261 gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca
    19321 atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg
    19381 ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta
    19441 taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc
    19501 ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag
    19561 gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg
    19621 ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta
    19681 ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat
    19741 cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat
    19801 cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat
    19861 aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa
    19921 tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt
    19981 tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat
    20041 ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat attatcagca
    20101 tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt
    20161 tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt
    20221 attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac
    20281 caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa
    20341 gggttgaggg agaaaaacat atgatcaggt tgttggaagg aaactataga aagatggaaa
    20401 aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac
    20461 gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg
    20521 gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg
    20581 taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat
    20641 taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca
    20701 tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt
    20761 tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt
    20821 tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc
    20881 caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt
    20941 ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca
    21001 tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga
    21061 taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca
    21121 gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga
    21181 aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat
    21241 ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt
    21301 ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg
    21361 cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat
    21421 tttattccac tctactgaat cagaatctga tttattattt atctagctat atcttttgga
    21481 tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca
    21541 gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg
    21601 tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc
    21661 ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata
    21721 tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt
    21781 tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata
    21841 cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag
    21901 tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt
    21961 acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa
    22021 tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct
    22081 ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag
    22141 atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa
    22201 tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt
    22261 ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc
    22321 gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa
    22381 aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa
    22441 gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt
    22501 gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg
    22561 ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt
    22621 ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc
    22681 caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc
    22741 aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga
    22801 ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt
    22861 aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct
    22921 ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc
    22981 agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga
    23041 aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc
    23101 ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa
    23161 tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct
    23221 gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca
    23281 atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg
    23341 cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc
    23401 ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt
    23461 gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg
    23521 accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg
    23581 gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc
    23641 gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt
    23701 tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac
    23761 gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa
    23821 ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga
    23881 ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt
    23941 aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg
    24001 attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct
    24061 cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct
    24121 tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct
    24181 gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt
    24241 aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag
    24301 attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac
    24361 ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca
    24421 tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc
    24481 aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc
    24541 caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag
    24601 tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat
    24661 gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc
    24721 agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg
    24781 tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc
    24841 attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag
    24901 ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa
    24961 agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca
    25021 gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta
    25081 tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt
    25141 acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa
    25201 agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt
    25261 tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat
    25321 tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag
    25381 gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat
    25441 taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg
    25501 agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg
    25561 atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg
    25621 ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca
    25681 tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta
    25741 tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta
    25801 gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg
    25861 cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta
    25921 ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga
    25981 cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc
    26041 agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac
    26101 catttcaaga gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat
    26161 tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg
    26221 cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag
    26281 aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga
    26341 tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc
    26401 ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca
    26461 attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat
    26521 gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat
    26581 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac
    26641 ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac
    26701 atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac
    26761 atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac
    26821 atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt
    26881 tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac
    26941 cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt
    27001 tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc
    27061 tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc
    27121 aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa
    27181 gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc
    27241 cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg
    27301 ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg
    27361 atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt
    27421 atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt
    27481 ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt
    27541 ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa
    27601 tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat
    27661 tcctcctagc aaatagattt atgattggta tattgttact tataaattta ttctatagga
    27721 gacgattgtt ttctacaaat atttagattg gaatttgatt aaaacaattt aattgaaatt
    27781 tgaaaacatt tatgttaatg aaagtcatat tctaataaaa tgtcatttgt ttctatatga
    27841 atttttatga aaaatttcat ataatttcca ctcgttgtga tggtttaatt tatccaatcc
    27901 atcaataagc aaacaactta tataaaa
//
LOCUS       DAYYHS010000030        27601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000030 DAYYHS010000000
VERSION     DAYYHS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.030"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACHA6A_001284"
     CDS             <1..1152
                     /locus_tag="ACHA6A_001284"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8969494.1"
                     /translation="MTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKVI
                     LATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAE
                     AIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIE
                     KIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGD
                     CATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAA
                     GLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDL
                     SGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            1264..2715
                     /locus_tag="ACHA6A_001285"
     CDS             1264..2715
                     /locus_tag="ACHA6A_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8969495.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3064..3696
                     /locus_tag="ACHA6A_001286"
     CDS             3064..3696
                     /locus_tag="ACHA6A_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8969496.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4284..5723
                     /locus_tag="ACHA6A_001287"
     CDS             4284..5723
                     /locus_tag="ACHA6A_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8969497.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            6018..6236
                     /locus_tag="ACHA6A_001288"
                     /pseudo
     CDS             6018..6236
                     /locus_tag="ACHA6A_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            6485..6700
                     /locus_tag="ACHA6A_001289"
     CDS             6485..6700
                     /locus_tag="ACHA6A_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969498.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6917..7173)
                     /locus_tag="ACHA6A_001290"
                     /pseudo
     CDS             complement(6917..7173)
                     /locus_tag="ACHA6A_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7360..8271)
                     /locus_tag="ACHA6A_001291"
     CDS             complement(7360..8271)
                     /locus_tag="ACHA6A_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8969499.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8290..9294)
                     /locus_tag="ACHA6A_001292"
     CDS             complement(8290..9294)
                     /locus_tag="ACHA6A_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8969500.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9291..11414)
                     /locus_tag="ACHA6A_001293"
     CDS             complement(9291..11414)
                     /locus_tag="ACHA6A_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8969501.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11419..13866)
                     /locus_tag="ACHA6A_001294"
     CDS             complement(11419..13866)
                     /locus_tag="ACHA6A_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8969502.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13853..14242)
                     /locus_tag="ACHA6A_001295"
     CDS             complement(13853..14242)
                     /locus_tag="ACHA6A_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8969503.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14302..14805)
                     /locus_tag="ACHA6A_001296"
     CDS             complement(14302..14805)
                     /locus_tag="ACHA6A_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8969504.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(14818..15042)
                     /locus_tag="ACHA6A_001297"
     CDS             complement(14818..15042)
                     /locus_tag="ACHA6A_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969505.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(15146..17056)
                     /gene="ltrA"
                     /locus_tag="ACHA6A_001298"
     CDS             complement(15146..17056)
                     /gene="ltrA"
                     /locus_tag="ACHA6A_001298"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8969506.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(17802..17939)
                     /locus_tag="ACHA6A_001299"
     CDS             complement(17802..17939)
                     /locus_tag="ACHA6A_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8969507.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(17936..19120)
                     /gene="mobT"
                     /locus_tag="ACHA6A_001300"
     CDS             complement(17936..19120)
                     /gene="mobT"
                     /locus_tag="ACHA6A_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8969508.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(19379..19669)
                     /locus_tag="ACHA6A_001301"
     CDS             complement(19379..19669)
                     /locus_tag="ACHA6A_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969509.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(19659..20801)
                     /gene="dcm"
                     /locus_tag="ACHA6A_001302"
                     /pseudo
     CDS             complement(19659..20801)
                     /gene="dcm"
                     /locus_tag="ACHA6A_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(20877..21041)
                     /locus_tag="ACHA6A_001303"
     CDS             complement(20877..21041)
                     /locus_tag="ACHA6A_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969510.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(21034..21708)
                     /locus_tag="ACHA6A_001304"
     CDS             complement(21034..21708)
                     /locus_tag="ACHA6A_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8969511.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(21786..23126)
                     /locus_tag="ACHA6A_001305"
     CDS             complement(21786..23126)
                     /locus_tag="ACHA6A_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8969512.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(23209..23544)
                     /locus_tag="ACHA6A_001306"
     CDS             complement(23209..23544)
                     /locus_tag="ACHA6A_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8969513.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(23554..23928)
                     /locus_tag="ACHA6A_001307"
     CDS             complement(23554..23928)
                     /locus_tag="ACHA6A_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8969514.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(23941..24255)
                     /locus_tag="ACHA6A_001308"
     CDS             complement(23941..24255)
                     /locus_tag="ACHA6A_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8969515.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(24368..27595)
                     /gene="fss3"
                     /locus_tag="ACHA6A_001309"
     CDS             complement(24368..27595)
                     /gene="fss3"
                     /locus_tag="ACHA6A_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8969516.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         8105 a         5659 c         4812 g         9025 t
ORIGIN      
        1 atgacactaa attctgagat aaaaaagtta tttctagaga cagaagttga caaaattgat
       61 attcaaaata aaaagattag ttgtgtaaaa aaagatggta caagattctc tcaagaatat
      121 gacaaagtta tattagctac aggttcaaag caaattatgc tagatatacc ggggaataat
      181 ttagctaata ttttttcgat aaagaatttt ccaaatgcaa aagagatttt taaagctttg
      241 gatgatagta ctattaaaaa agtaggaatt attggagcag gatatattgg agttgaaatt
      301 gctgaagcca taagaaaaag agggaaagaa gtatatcttt tcgatgttgc agatagagtg
      361 ttgtcgactt attacgaccg tagtttttca gataaagttg aagaaatact atcaaaaaat
      421 ggaatacatc tttgtctatc agaaagtcct aaaaaatatt taggaagaaa aaagattgaa
      481 aaaatagtca ctacaagtgg tacccaatac tctatggata tggtcgtaca agctatagga
      541 tttctatcta atagtccaat tggggaaaag gaactcttga gagatgttag tggagcatat
      601 ctaactaatg aataccagca aacaaatata aaagatatat atgctattgg ggattgtgct
      661 acaacttttt ttacgtccat taatagaagt agtgttgatt tttctatttc taatgctcta
      721 agaacagctt atattgcaac tcaacatatt aacaatcaag attttacacc ttctggttcc
      781 caatttacaa atggcttttc tatatttaat tacaattttt acgcagcagg tttgaattta
      841 aaaacagcac gattattaag tgagtctata gattatatag aaatagaaga tttcattaga
      901 cctgcttttt tgagaaaaaa tagtcgtgta aaattaagaa ttagctttga aaaaaataca
      961 aagagaatag taggtgtaca actttgtagt caagaagatt tatctggact attatcaatg
     1021 ttttctcttg ctatagaaga acaaattaca ttagacaaat tgaaaatgtt agattattta
     1081 tttcatccta gttttagcca accctataat tttattttaa aggcagtaac aggattaaac
     1141 attgaaacat aatcgttact aaatgaaatc aagaaatata ctgtattact caattcattg
     1201 aaaatattta tcataatttt taatagaaaa cactattaaa gagaaaaaag agaggtaaat
     1261 caaatgaaaa aatctttaaa acttgcatca gcagttatat taatgtccca actccttata
     1321 gcaacacctt tccatgtgtt ggcaagcgaa aattcaacat acaaaattgc aaaagaaaga
     1381 accgagacga ctgaaatgag taattccgat gtaacagcag agagtagtga acttttgaca
     1441 acttcatcaa gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcatcaagt
     1501 gaaagtagta cgacttcaaa tgccgagtct tcaacaactt catcaagtga aagtagtacg
     1561 acttcaaatg ccgagtcttc aacaacttcg tcaagtgaaa gtagtacgat ttcaagtacc
     1621 gaatcttcaa caacttcaag tagtacaagt gaaataactt cttcgaaagt gacaaaacca
     1681 aaccatatag aatcttcaaa aaaggaagcg ccaaaaacgg aaggacaaac aaaaactttt
     1741 tcgacaccac agcaaataga atcaacagaa tctttccaat cacaaccaat actatctggt
     1801 aatgaagaaa ttcattttga caaaaatgaa tctgtagaaa attttattat gaaaattggt
     1861 gagtctgcga gaaaaattgg tcagaaatat gatttatatg cttctgttat gattgcacaa
     1921 gcgatcttag aatctgccag tggtcaaagc cagttagctc aggcaccgaa ttacaattta
     1981 tttgggataa aaggaactta taatggaaac tttgtcatta tggtaacaaa cgaagatttg
     2041 ggtaatggaa cgctttatac gacccaatca aaatttagag tgtatgaaaa ctatgaggaa
     2101 agctttgaag attatgccaa attattaact aagggaatta gtggaaataa agatttttat
     2161 gcaggagcat taaaagctaa tagtaaaaca tatcgagaag caactaaatt ccttacagga
     2221 cggtatgcaa cagatacaca atactatttg aagctaaatg aattaattaa aacctacgat
     2281 ttaaccaact atgataaaga agttaaaggt ccacaaatga gtaagactgg ataccaagtg
     2341 cctattcata attattcaat aaccagtttg tttggaaatc gtggaaatga atttcaccga
     2401 ggacttgata tggcagctac acagggtgag ccgattcatg cagttaaagc tggtaaggtt
     2461 gttaaagcag agtttcacta ctcttgggga aattacgtgg tgatacaaca cgaagatgga
     2521 tcaaccgcgt tatatgctca ccagcaacaa tacctagtaa aaactggaga taatgttttg
     2581 caaggacaaa taattggcta tgtaggaagt actgggaata gtactggccc acaccttcat
     2641 ttggaaattt gcaaagatag tagtctttct cagagcatgt tagttgatcc gaaaacctta
     2701 ttattcggag actaaaaaag gtaatcaatc tgacaaagta acaagaaaat ggttggattc
     2761 ttagaccttt gatcaggata ttgagactgg aattgatatc tcgattacga tgggtttgac
     2821 aaataaaaca agtccattat cgaatatcga gtgtattatt gccgtcttta tgctcatatt
     2881 ttgagcaggt ttcactagat gaaaacaatt tatctatctt gaggatttcg tgtacgtatc
     2941 gttcagtctt gtattgtaaa ttagtcataa aatcagacta agaaaaattg gaaatatttt
     3001 tttctgattt attaaaagtt aattgaaatt atatggtact tctttcttgg gagggtaggt
     3061 gcagtgaagg aaagatatgg tcaccttact gttgttgagg agattactca taatggatat
     3121 aaaaaatatc gttgcctatg tgactgtggg aatgaaacga ttgtatatcg atcaaactta
     3181 ctatcaggta gaaccaaaag ttgtggatgt ggaaattata aaaataggca taaatataaa
     3241 aatttgacaa ataaaacttt tggtaagtta gttgcaatca agccaactaa actgcgaagt
     3301 gaaggaactg ttgtttggga atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca
     3361 aagcgacttc taaaaggtga tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac
     3421 attgaaaata aaaaattcgg attacttact gcaaagtatc ctttggacaa taactgttca
     3481 tggaaaacaa aatgggtttg tcattgtgag tgtggcagta tgtgtgagat aagttatagt
     3541 aacttatttt ttggacatac tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca
     3601 tgtgttgaag ggacagtaat agagtgtcta acgtcaaaat taccgaaaaa taatacaagt
     3661 ggtgttaagg gagtttatcg ttctaaagag aaatagattg cgtatataac tttaaaaaaa
     3721 aagaggtact atttaggatc gtatgattct attaagagtg cagctcaagc tagaaaagat
     3781 gctgaaaata aattgtttca acctttgatt gacaaatatc gaggtaatca ttaattattg
     3841 tggataataa ttaacagtga tatataaaca tctataatat attattcata ctattgatat
     3901 ttagtataat cctatggaat tgaatatcat agcaatgtat ttgaaaagca aattggaaaa
     3961 caaataggtg caacaccata aatatgaaaa aattgggaaa aagtagaatg gaaatttgct
     4021 tacgtctcac gagatagctc acactattaa tctgaagaac aatgaagtcg acttttagaa
     4081 attgctagaa gaaagtaaag cgaattatca ctagttacta tagagtaagt tcaaataaag
     4141 catctccaac ttacatggtt acatgaggat atcaaatcga aattactcaa gcttcctatg
     4201 gctagagtcg cgagaagttg ttgggcagta gacataggtt atattgttct tagctacacg
     4261 atcattagaa aggaaatcct attatgtata gttatcaaga agatgcaaaa gtccaaaaaa
     4321 atcgctggtg gattttgatt gctatatcta tgtttacctt catgtccaca ttggatggca
     4381 gtattgtcaa tattgcacta cctaccatct cagatgatat gggcatacca atgaatcaag
     4441 cggaatgggt agtgtccgtt tatctgatga ttgtctgttc ttgtttgttg ctgtttggta
     4501 aaattggcga tagccgtggg aaaatcaaag tatttcggat tggtacgctg atctttgtct
     4561 tgggatcgct gttgtgtggg ttcaatcatt cgctaggtat tttattgttc gcacgggtgg
     4621 tacaagccat tggtgctagt atgactatgg ctacaaatgc cggaatcatc acggaagtct
     4681 ttcccataca tgaacgtggt cgggcattgg gagcaatagg tgcgtttgtt tcattaggct
     4741 cgattgctgg accaggtctt ggcggcttga ttttgtccca attttcttgg gcgtacattt
     4801 tttggatcaa tgtaccggtg ggaatcgtaa cgatgttgat tgctgaaaaa ttcttaccaa
     4861 aagatatcac catgagcggc catacaatcg atggaggggg ctttgctgcc attgcggcta
     4921 cgattatcag cttttttgga gcaatctttt tggggcaaga aagtggcttt ttccatccag
     4981 tcccccttgt actgttcgta atttcagtga ttgcctttgt cagcttttta aaagttgaaa
     5041 agaaagcagc ctctccattg atttctttcg gtatttttaa gaacaagatt tttaccatga
     5101 gcttgattac agccttgttg attttttctt ccaacttctt tgtcaatgta gtgattcctt
     5161 tttatctgca aaatgctcgc gggttgagtc ctagctatgc tgggctgttg atgatggtct
     5221 ttcctttgat gatggtcgtc ggggcgcctt taagcggcta cctgaccgac cgggttggcc
     5281 cggagatttt ggtattagta ggcttgggct tattgtcggt aacccaggtc atgtatatgt
     5341 ttttgtcagt ggactctcct ttgtggctat acattgtcgc tactgccatc atgggtgccg
     5401 gcaatgcctt gttccagtcc cccaataata cgatggttat gagctcggtc actatcgaaa
     5461 acttgggact ggcaggctcg atgaattcct ttgcccgaaa tttggggatg gtcagcggga
     5521 tttcgctttc tacgacggtc ttatatcaag cgatgtctgc taagatgggc cagcaagtaa
     5581 ctaactatat cgcaaatcgg ccggatgtct ttttatatgg aatgaagatt acctttttag
     5641 gcagctttgt tttgtgtttt gttgcctttt tcctgactat ctggcgtatc aaaaaacaag
     5701 gaggtattca gaaaaatgtc tgaaaaaacc actgttgaag aatttttgga ggcctgcgta
     5761 tgggtgagtg ggatttccat ggatgatgga agatgaagtg aacttctaaa agccgaaaaa
     5821 ctcaagtatt atcttcaaaa tactagtgct caatgagatg cttgatttga tcacaagaag
     5881 actgcgaaag ccgctggcaa ccgcaagcta ggtatcccac cgatggacta cgttttagag
     5941 gctactggtt atgaatacgg agccaacgct ctcatctgca taagaataca tcacccagat
     6001 tatgtaaaga ttttcgatac ttctgtcaaa aaatcattaa gatagataaa tgatttccgt
     6061 ctagtcaaat tggttcagaa tgtagacaca cagatggtaa gaagtcactc acaattcgag
     6121 aatggacttg tcctatttgc cgtatacatc ataatcgaaa cgtcaacgat agtatcaata
     6181 ttttgaccaa gggctatgaa tacaagcatt gccttagaaa acatcaagaa ccttaggaac
     6241 aacggggata gcttggttaa taagagatac cgctgtttag gcttgcaagt atactctttt
     6301 atcaagaatt ccgtggcttt agtcatgaga ggcttaatac tatgaaaatt ttctttagca
     6361 tttttgattt aggttccttt tctaattata ataagatgac cactacagta acctactgaa
     6421 atatataaac agccactgta gtaccatttt caaagtagtc tatgagagga gaatcttcat
     6481 tcttatgaac gctggaattg cggatatgaa tttaataaaa aaaaccctta atgattttac
     6541 ttccaattca atatcaaaag gtactggtat caatttaagc acaataaaaa agctgaaaag
     6601 tggtgaaaga tcagtggaaa agctgaatct tttagatgct ataaaaataa cggaatttgc
     6661 tatgaaaaat ggaaaagctg agattgaaat atggagataa ttatcgtcta gtgaattact
     6721 tgccacttag ctgaatttaa cgatccttgg tgcttgaagg gggagattct tgggaataga
     6781 gcatacttgc aagcctatgt ctttactaac aacgatatct cttattaacc aagctatccc
     6841 cgtagttcta aggcttttac ttttatctaa gcaataatcg tattcaaagg aaatttttga
     6901 cccttagaaa gtctcatttt atcagacgtg agcttaggca ttgctgaatt ttgttaattc
     6961 ggttttaggt tgtgctttga gcataatatg aaagtattct ttatcacgat ttcattcatc
     7021 taaggtaatg tgatgtgatt ctgaaattct ttcaaaggca gtttttgcat aagctgacat
     7081 tacatcatca atgacatgtt tacaatgttt cactactaaa ataagggggt aaccaagaag
     7141 aaaaactgaa tgattatttg tatctaattc catggtatat caaccatttc ctatatagac
     7201 tgattatgac atggattaaa ataaaaaggc actatgtctt aattgtcggt tttcgagtta
     7261 ccgaatggca ttcatcaccc acctatagag gatgggcgac ttctgcctga aataagttac
     7321 actacttata acttctaata aaattagatt gagtcattat tatttaacat caacgatttt
     7381 ccagtttcca tttttttgta atactaattt gtactgaaag tagctagtgg tttttgatac
     7441 attatcaagg tattttatct ttgtactcac tagaaaattt tttccgtttt gtagataaac
     7501 tggattctct aactccgaaa actttaagtc aatattgatt ggtcgcatcg caccattttc
     7561 aacataatac tccaattctt tttcagaagc tgatggataa agtttaaaaa atgtttctaa
     7621 aaaatcggtg atttcttccg ttgttttggc atcaatttct gaatcgtttt ctacttgttt
     7681 tgcttcatat tttgccttgt ctggctttcc aatcattgtt ggcaaactag ttactaaatg
     7741 attcctgttt tcatcttcaa agattgtcac tcgatatgca gaagaaattg attgcgtctt
     7801 ttttccctct gtaatatgtt gctccactaa aaatgaaacg acaaattctt ccgagtgttt
     7861 ttcaacatct agaattttta cagattgaac ttcggaactc gtgggaatat ctgctcgaac
     7921 catatcttgg attaaagcga gaccgttttc tgtaaggtac tgttcgagat tactcattct
     7981 cttttcaatt gcttctttgt tatttttcca tgaaaaatat tctttcgcaa aatcctctgt
     8041 aaaactttca acgccagaag tattcactac cttctctttt ataaccactt tttcatgagt
     8101 tgtatgttga tcaatggcgg tgaaattttt ataaacgcca aaagcaagac tacaagacaa
     8161 taaaagccaa agggcaagca ccatttttcg atgcttaccc acggatacaa ctcgttcttt
     8221 tttctttttt gggctctttt ccttttgatt ccgttcaatt tttatcttca ttctttttcc
     8281 tcttttctct tatttgtatc ggccagctcc gataagatgc tgccgccaat agttttccgt
     8341 tagattcgtc caccccactg gatctcctgc atgaaacatg cgattctccc ctgcgtaaat
     8401 gcccacatgg gtcacataag taccggcatc ataggttgaa tgaaagaaaa ctaaatcacc
     8461 tggtttggca tcttttagat caatatgagt catggcatca tattgatcct gagccacccg
     8521 aggaagtttc aggtcaactt tggcataaca ccattgggtc aagccactac aatcaaaccc
     8581 cgtttctgga cttgagccac caaacacata ggccgttcct tcatacttaa atgcctcatc
     8641 aaaaatttct tggactattt tgtcatcaaa ctgagttgta gttaggtatt gcttcactaa
     8701 taaacagtaa aacatattgc cataattata ccgccaccca ccattttcct taatcgcgat
     8761 tgggttgggg taatcgactt tttgaccacc tgatttttcc tttgaaaaag attcagctaa
     8821 agcaaaacta tgcttcttcc cgtttttccc gacatgatct agatacccgc caccatagtt
     8881 ataggcctga ataatactat cttgatcaca ccctagttct ttggaacgct taaccaaaga
     8941 cgcaaaatag agacagcctt gtttgatgga tgcctcggta cttaatgaat taggtggtaa
     9001 tccggctgac tcagaacttt gcataacgtc ttccattgtg ccaccagatt ccacttgaat
     9061 aatcgccaaa agaattggaa tatactctgt tatatccgat tctttggcat acttttccac
     9121 cattgcacgg tgtttcaata cttcttctga gacactagtt ccgccaggag aagtgccaga
     9181 actaccattt ccctcactgt cttcaccaaa caacaaaccc acacacatca aaatacccat
     9241 aaaagagatg gtcagaaaag ttaatgaaat aacacccact ttcttcaagt tcattttttc
     9301 tcacctgacc ttgattttgg acgatggttc attctttttt gtactttttg attggtctgc
     9361 tttggcagag tggacctctg tttcacttgt tggaaagaat ttttcttact tgctgattgg
     9421 cttttccctt ttgaaatgat ttcttgacta gttagttttc gtttgatttc gggattcaga
     9481 tttgctctca caatccgctt tggtgcttct tttttcagca tcatatcttt ctgaggttga
     9541 gctgctttat ctattggaat attcgccctt ctttttccct gacgatttgt tgcttcactc
     9601 agtatcttgc gtttttcgtc cattttcgca tttcttcgtt gtcgttgttt ttctcgcaaa
     9661 tccactcgat ccgctttttc ttggaacagt ccgcgcttga attcttctgg tgcttttttc
     9721 gacttttcga ttcctttatg gagattgtat ttcaaattcg ttggcgtatt ccttacctga
     9781 tcctttactt gtttagtctt atctttcacc aaagcttggg tatccaaaac tttgccgatt
     9841 tttctcccag ccaaattcat tcgggattgt ttcttattat ttgaaactgg attctcctta
     9901 gttaagtcag atagtatttc gtgatcgttc ggtaatcgtg aattctttcg tagagaagaa
     9961 ccggatggct taaatttgga ttttgattgt tcctttgcca acttatttcc gactgctgca
    10021 ccaccaccaa aagccaaagc tcgtcccaca tttcgattga gttgccgcaa ttttcgattc
    10081 attaacattt gcggttttcg catgacgcgt cttcccattg actggctatc attactctgg
    10141 aggttgaaca tactcataat gtcgcccaat ttgaaataga taccggcaaa ggtcacaatt
    10201 tgcagaaaag caaccatgaa aaatggatag gttgccgaaa tattgaaaaa catcgtggag
    10261 atgctaaatg cagtcgtaat caccaaagta acacccgctc gcatcataat cgtattgaat
    10321 aaacgaataa tcgccttctt tcctaaactt tcataagtcg gcaacatgga caataagaaa
    10381 ctaattggca gaaacatcgc aaagataatg aagagaattt gcgagaacaa cataatccct
    10441 gttagaagaa aaacaaagaa ggaaatgcca atattgaaca ataccaaaaa gaccacggtc
    10501 cctaaacggt tcatggtctt ggtaatactg agattcgcat tgtcattgtc ttcaatctct
    10561 gccttgaccg cttcttcccg atctttgcct ttgttttcat ctggactcac agatagaatc
    10621 ttgttgaccc ggtcttcacc aatttcttct atgttggaat catcaaattg cagtagtaac
    10681 caaggttgtt ggacttgaat cgaaaacaaa ctatcccgaa tcaaatccac actatctttt
    10741 ccttggcttt ctgaattggg tacaacgatc tcggttccaa gggtaagggc cgcttcacta
    10801 acatctgagc taaagtcatt gatttttgtg atataggtag gcgcataagc aatgaacgaa
    10861 cccgatagta gaaaaatcat cacaaaatta atcacggctc gaactgcttt cgtggtttct
    10921 cgttttaata atccggtata cgccacatac acccccagag ctaaaatcat caataataga
    10981 aacccaaaat aaaatccgga ggtctggagt ccattttcag taataccagc caatgtttgg
    11041 atattctttc caatactttc agccgtatcc gaaatgaaat ctaatttgta agcctcttga
    11101 accacataac ccgtggcgtt cgacaaatac aaactcaccg tccaaatgaa attagtaatg
    11161 gcataaagac catacatcac gcttttccct aagccatcac cccaattcca aggcaaccaa
    11221 tcccaagagc tatccacaaa gaaatctagt tgatagttgt tcagtgaata tttggaatat
    11281 agatttcctg cttcaactgt atcatcaact agccctgtcg cctcggctac cgtaccaact
    11341 aaactcaata gaactatcag agtgagtatg ctaataaaaa cgattcccac aatccgaaaa
    11401 agtttctttt tcatcccctc actccattct ctcttgaaca ggtggacggg tatcaaaggc
    11461 atggaagagt tcttcaaaga ctggatgtac ctgaaccacg cctacccgac catacaaatc
    11521 ctgcatcaag cattggccat tttccaaatc tcgtaggcgt ttttgattgc tttcatcttc
    11581 tcgatccaag ccaaagaatt ccaaggtttt cttgatttca tgaatatcgg tactgcgaaa
    11641 cgcaaacttc aacccgatat tattcttcaa gcgttcatcc agtaaatcat cactattttg
    11701 cgtgacaaag taaaccccgg cattcatagc ccgcccagca cgaacgagct tattcgataa
    11761 tgttttccct tgcgctactt gaagaaaact ccacgcctca tctaagtcta caattttgaa
    11821 gatttctcga ttactatgga taaagtccaa ggcaaaagtg gaaatgacga ttagcatgga
    11881 aacactaagc aattccatgg tggtatattc ttcaaaagtg gtttctgcat ctggtaaaac
    11941 caaatccgct acttgaatga tatttaactg cctatctaaa ctaatcgact gggtaatcga
    12001 accatctgag aaaagcaaat gggcaaaatc ataatcggta aaggactcga tgtgatccgc
    12061 gatattttcc gcaactggtg agccttcttt tcgaagctca ttaattactt gtaataatcc
    12121 ccgttgattg ctttgggtaa ccgtccgaat ggccttacgc aacactggaa acttctcccc
    12181 atctcgacta gaaattcctg ttaaaaaggt cagaatatca attgctagac tttcagaatc
    12241 cttctttttc tccataatca cataaggatc aagtaatcct tgattttttg gcttactagt
    12301 taagttgaga atattgattt catccgcaat ttctggcaag gtttctttcc agccaccccg
    12361 ttcccctttt ggatcgacaa tcacagcttg tccaccaaaa aggacggcgt aataaactaa
    12421 gagattgtta ctaaaggact ttccgccacc gagtgaaccg aggaatgccg ctgctagcgc
    12481 attggtaact gaatccttga ccccttgtga ggccaatgcc ggtttcaggt aaacattgcg
    12541 ccccgtatct acgttataac caaagtaaat gccttccaac tcccctaaca tctgagtggc
    12601 gccaaaacca agtccggcga gaaaatcaga cgtaacatac tggatataat cattcatgta
    12661 tcgcttacta gaaggtaaga attcttcatg tagccccatc atatcgccaa aaggccggac
    12721 tagttttaca ttggtatcat cataaaaatc tagcacttca tcacaccggc gctttagttc
    12781 atctactgat tcggcactta ctcgaaccac atagctcagt ttgtacatgg attctttgga
    12841 ctgatccaag gtagtttcca attcatctac ggaatccaat gcatctaaga cattggaatt
    12901 tgtttcattg tcagattgat aggcatggtt gtccaagtct tttaattcct ttttcttatt
    12961 gcgaacggta gcaagtgcct ttttgttcgt cacaatctcc acattcatac tagtgtcaat
    13021 tgggaatgta aattgttgtt gctggtaata gaaaagttct gaggagggaa actccatttc
    13081 ccccacaatt gtattaattg ttaagtacgc tacatatgat tcatgattct catgttccat
    13141 gcataagtac cggggctttt cttcaatcaa acaacgactg ggacgtaata agtcgtaacg
    13201 tttcaccaaa gtttccgact tcagcttttt ctttggtagc tgaaattcat attcttcaaa
    13261 tggtgtaccc ttttcgccat agatatgttc aatcaaatac atcagatcac tcggtgtgac
    13321 tcgtctgact ttaaatcttc gagccaattt actttccatt agtttttcaa gcttggtata
    13381 tcggcgaatt tcttcattgg ataaggaaac aaaatcgccc atcaaatgat gattcactcc
    13441 atagacaaat tcttgaaagc cggagaagaa agatttcttg agattcttta gattgacctc
    13501 ttcatctgtc gcaatcagtt tgaagccaat aaaaaagcga taatcaatct gactatcgcc
    13561 aatcatggat actaatgcct ctgtctgtaa atcaattcgt tgtttcgcta cttctgttaa
    13621 tcgcccggta atttcctttt ttgatcgttc ttgcgtggca cgaatactac tttctgtggc
    13681 aatttgtaag gcatgaatct tgccttcacg attttgcgca atcaattgac gaaagttatc
    13741 gtggacttga tatttctgtt caggtgacaa aaaggagtaa ttgtaaggta ttaactcata
    13801 gtaggcaaaa cattctccct cttgattaaa gacaagatta ttttcaatat acttaatcgg
    13861 gtacatagtt cacactcctt accagagtaa tgggttcgtc ccatcgtttc ttctgttctt
    13921 tgactttctt tcctgcgaaa gtgaccttaa atcgtaagtg atagctaata aaagatcgta
    13981 aaaatcccaa tggtttctta ccgtcaaacg ttttttgcga catgaaccaa gtaactgcca
    14041 ccggtatacc taagtatttt aaaaaggccc cctctatcat agatagtgga ggcacatggg
    14101 caaatacgat gacaaacagc aaggataaaa caaaccaagc catctggtta aaagtcacag
    14161 gaaaaggcag ttgaaagtca ttaattgcat aaatgacctt ttcaactgcc caaatactgg
    14221 tgtagctttt tattttcttc atcaaatcat tcctttaaaa agaaggatag tttagaaatc
    14281 aactaccctt caataatttt gttaatatgg atactcaaaa acacctcgac tcgttaccaa
    14341 ataattgcct ccaatctcca agtctctacc acacgcatca aaatcaatat acgtcttcaa
    14401 gtgttctggt acttctccaa aatcaccagc ttctacaatc aagaaatatg ctacatcact
    14461 catatcatcg caatctggat aatagataat atcatccaca tgctctacca tctcctcaaa
    14521 agaattgaag aaagcatgtt gaatctcaga agcaacttca ccaactggcg ttccttcaag
    14581 ttcctccgct aagccacaca accgattaat ttcctcaatt ggcgtatact catcaatctc
    14641 aaagggaagt tcataatcat gaattgcata ttcctcgtat tcgtcattta acccaattcg
    14701 ttctttcact tcatcaaaat cgacaggtgg ggtaaaccac gcccccacca attcaccttc
    14761 gttatatttc ccgaggttcg caatataaac acgcatctgt tccatcgtgc ttccccctta
    14821 cgcaccgaca attcggttaa acaaactgag taagacatcc ttcaccccag aagcattaaa
    14881 gaccaatccg accgcaatca aagcaatcac caaaaagcca atcagtttgg aaaactcccg
    14941 cttaaatcct aaaaacaaac caatgaccac aattgccagt aagactaagg attgggcatt
    15001 ggttaaaaac cattgatata tggataggta agttcctgat attataaata tctcaaactt
    15061 ttccccccat tcacacggag cgtgcgactt tcaccgcact ccgcgttcca tcgtgtttat
    15121 attgaaaagt atttatgtag tagacctata tcaagtttaa ttcttgattg ccagctaata
    15181 ctctatattt atttagtctt atgagctgga ctttatttag cttcaataga gctatatatt
    15241 tttgtattgt ttcttctgtt ttagcgtgta ttagacggtg taccgcctct tttaccaaaa
    15301 taagattttt ataatcgtca tttccaccag ctccagttgg tacaatatga tgacaatgta
    15361 tttcattagc ttgttgaaat tcttccagag ttatagcaca ttttccatgt tgagcagaaa
    15421 atagcgaaat tcgattgtct gcgtattcta ttgtgcgatt ttccaaagat aatttcatta
    15481 actggtacat cagtaattta tcaacactta gattggtgtg gataaatttt cttccctcta
    15541 gagtgtagca attcgctaaa tacacctttg ccattggttt tctgtgttgg atatatccaa
    15601 cagggaaaat aggttcgccc tcgccagcaa cataccgaag cattttagat ttaccatatc
    15661 ttgttgcctc ctttgtcgta agttgtctgc ctgtttttct caatctgccc cttttgtttg
    15721 tacctaatct gttagtgaag atagccatta cagctctatt cagtttcata caatccagat
    15781 ttacattcgt tgcaatctga taataatttt gcatacctaa taccattgaa ttataaagtc
    15841 ttgtttcttc taattccgtt ttcccttttg agggagaagc aattttcttt gcctgtgata
    15901 ccagcttgtc tttttggttt ttgaattgtt tatcacttat atgtgattta acaactaact
    15961 tctttcttct cggtatcatt ttaatcttga aaccaagaaa gtcggagtat ctttttctaa
    16021 catttacaat tcttgttttt tcttgtgata cttccaattt taatcgttgt tcaatccatt
    16081 gagtgattgc tattttggct ttttcagcac tttccttgta gcggcagaaa actctaaaat
    16141 catctgcata acgaaccata tacatttcct ttaactttgt tttcttcatt tctttgtaag
    16201 cgttgctttt acatgggcta ccgcttttat tgatatgaac cacatagttt tttgtaacag
    16261 gattttcctg ccattggctt tctacccaat ggtctaactc attaagaaca atgtttgcta
    16321 gtagtgggga tattattccc ccttgcggag ttcctttctc tggatatgtt attgtaccgt
    16381 tagtcatttt gattggagct tttaatatcc tttttaaaat gaatatcaag tgcttgtccc
    16441 gtattcccat agcccatatc tgttttatca gcttggaatg gttcacgttg tcaaagaaac
    16501 ccttaatatc aaattcaatc acataatgta gctggctgat ttgtaggcgg ttataggttg
    16561 cttttattgc attttcaact gacctattag gtctaaaacc atagctgttt tcactaaatt
    16621 tagcttcgca gattggttct aatacttgtt ttatacattg ttgtataagc ctgtcccaca
    16681 tacaaggaat acctagaggt ctggttttac catttggttt tggtatttct tttcgcctca
    16741 ctggcttagg tcgataccca tgtggactgc ctgcaacaat gtatcttact tttttgacaa
    16801 ctgtttctgc tggcaatttg ccaatatctt taattattgt accgtctgtt cctgctgttt
    16861 tactgcctgc atttgctttg atatttcgat atgccagtaa tatattttct ctcgatagga
    16921 taatatccat aagatgagtg aaaatatctc cattcttact tttggcatat aaatcatcaa
    16981 aggtgttttg catttcgtaa tattcagcgt gtcttaaatc ttctacacac aatgtttcat
    17041 ttttattttt aggcataagg cttcactcct ttacttgaaa ttgagtgagc ttttttatct
    17101 tactcgtaat ccttattgaa acaacataat acttttctaa tatatacacg acttgagggc
    17161 attcctccat tctcattaca agaatttctt aggttcgccc tctgcttttc agcattagtt
    17221 caactgctta ttgttcttcg tcagtttatt ctcttgatga gaacccaata cctttcctcg
    17281 ttccgataat cttatctata catatatatc cttaggtcac tagctataag cctgctggct
    17341 tgataccgcc tgtaacgtta tatggcattt cataacctat attcttactt tgccacacca
    17401 gctacgattt ctcgtacctt tgcatttcta caaagttcaa atttagaccc gtacattcgc
    17461 tgtttcgtca gttccttata gaacattcta accatagata ttttatagac ccccgacctg
    17521 ttggatacgc tacccacctc tggatagttt tcgatagaag atatatgttc atcaactatt
    17581 acggtatctt tcagccgtct ttaccgagct tcaaacattc tgagtttcct catacaatgc
    17641 ttgtcggagg ctcagggcag gcgtttcaga gcgttacttc atcattccac ctgtcagatt
    17701 atcagttctc ctaataacca aactttttcg ttcttttatt agtgccttag cttttcacta
    17761 aggaacgagt cgcacataaa ttttgtccga aattcatctt gtcactttcc tttcttttcc
    17821 attgctcgat ccgctgctgc acttgcattc atcaagcaca tcacagtaac accaagcata
    17881 ctgccaccaa ataccaaaaa aatatctttt acaattccca tgacgattcc tcttttcata
    17941 taatcaaatt ttcaattgcc atactttgtt gctccaaaat ctttttatgt cggtctgata
    18001 agcgtgcatt ctgtaccata tcctggatat aggttgtccg attcaatcga tccaactcct
    18061 tagccatttt ccacgtaggg gcgacttgtc ggcctagcca attcaaggtt cgttcaatgg
    18121 tatacggttt tggctcggaa gttaattgaa tctctttccg attccgtcca ataaaacgcc
    18181 cccattgttc attcaagggc cagttggttc gccttttacc ttctacttta tccgcaaatc
    18241 gcaagtaacg attgataatg gaaaacgtgg tgctctcaat gctacgacct tttaacaaat
    18301 cttgaatcgc atgaaaagca cggtcatttt tcagtcgaat ctcaaaacga tttttcgtct
    18361 cagtttggtc taatggaatc cctaacttga tatattgttc atagtctttt tcatacaaac
    18421 agaaataaac ctcactcttg agactgccga tatataaggt attgcccatg ccgtcctttt
    18481 catcagcctt taaaagttcc ccggaacgat aacttttaaa ggtacgaaac aaggagatac
    18541 attcttcctt ctgacatttt ttcgttagct caggtatatc caatagcccc actcgatcat
    18601 tgattgccaa gtccaagcgt ttcatcacgc caccggcctt tagacaatcc tcgaaaaagt
    18661 cgtaccagct acgcttttga gcgagtaaaa aagtttcaaa ttgtcggcaa cctcgcccct
    18721 ttaattctaa aagcacacct ttatcttctt catgagacaa catcaccaca atatcaccca
    18781 tgacatactg ttcttgatag gagtaaaagg cataatcttc atggagcata tactcaaatt
    18841 tcaaatggag aatttcttga ataatttttc gtgcatccgt tgtgggaaag cgaatcctta
    18901 catagtcaaa cagtaacgtc aaaggcaaat cgggattaaa gctttccagt tggtcaaaaa
    18961 ttttcctcat ggtttttata gatgcggtta cgccaccatt ttctatctga tttaagtacg
    19021 gccgactaag tccacaagcc gtagccaatt tattttgtga aacgccgtat tcgttgcgtc
    19081 ggtcttttaa tagccgataa tctaaattcc gttgtgccaa gttctcacct ccaaattcaa
    19141 ggtgttaact ttttcaaatg agttaacacc tatacaaatt acttactatc tttgatacaa
    19201 caagcattct taatggttgc ttacttattt tttgtgtttt tgtacccccc tgttagatac
    19261 gggggtgaga catcatggtc ggctaacgcc gtccatgaac aggcacagca ccacggagga
    19321 tttctgcttc gcgaatcctc cgcggtcagc gcctaacagt cagttgtgtt ggtttaggtt
    19381 actcatctgt aataccattt acttccaact ctgctttcat ttctaacaag tattgacgaa
    19441 tatctggttc ttttgcttgg tccgcaatcc gctcaaattc tgcaattact tgatttattt
    19501 caattcgagc atccgtctcc tcattcatta aatatggatc gccagcgact ttgacaccat
    19561 tcttgcgaat atcaaatttt aaccgtcgca attgttcttc taattctggg tctgactgaa
    19621 tctttccaat cacccaataa atttctccaa tcaactcatc ataaaccatg tacatccaac
    19681 tcttttaatc ttctggcaat atcaaaaatg accggaactg ttacagagtt tccggcttgc
    19741 ttatacaact gactatctga attgacttga gaatctcgat caaaagccca atcgggaaag
    19801 ccctgaagtc gccaacactc tctaggtgtt agcttccgaa tttgatactg atccgtgacg
    19861 atcgcttgtt ggtctcctgt tagtaaggta tgaacactct gttttcccag acgacctcgg
    19921 cgacttgtgg aattaggaaa agccagattc acactgtctt ctggcttcac ctcttgatat
    19981 tctgtttcat tttttcgaat catgattttt gtaccacctt gtttcgttga caaggtgggg
    20041 gcggttccag taacttcata aactcgatta ccttgttcaa actttcccgg taatagtcct
    20101 gctacagaga ttccatccct tgctggcagt gcgattttga ttccttcatc acctgttaag
    20161 gttggcgcta cgccatctga accatagact tgtccaccca ttcctcgatt ggaaggattg
    20221 atatttacca aatttctgat gttttgatca gatgttgaag acggtccaat gataggaaat
    20281 acttgttcgg tacatgcgtc tcgagaatgt ccgataagga atatccgctc ccggctttgg
    20341 ggaatgtagg cctttgagtt aagcacctac cactccacgt cataccccaa tccatccagc
    20401 gttcggagga tcgtctcgaa cgtagcccct ccttcgtgat tgagcaatcc tcggacgttc
    20461 tcaaggaata aaagcttagg tcggagaaga gtggtgaacc gagcgatttc aaagaataaa
    20521 gtacctcgag tatcggaaaa tctttgccgc tttcccgcaa ttgaaaaagc ttggcacgga
    20581 aatccgccgc aaaggatgtc cactggtccg agggattgaa taaactcatc tgatacactt
    20641 gtgatgtcat gcatttccac ctccttactt gtatcatgaa ttgctttata actacgtcta
    20701 gcaaatttat caatttcaca aaaaccgata caatgatggc tcgcctgttc catacctaaa
    20761 cgaaagccac caataccggc gaataaatct aaaaatctca tacattacct cattcattgc
    20821 aaagaaaaaa gccaagaaca ttttgctctt ggctcataca ttattaattt gtcctactat
    20881 tttttattta atccaactaa tttccgttgt ttttcattgg cctgatcggc tacatattta
    20941 atcaccgttt tgaactcttt tgcagtagca ttttccgata aatccgcaat gctcttattg
    21001 tatttcttta caattttcgt tacttgttct ttctcagtca tgtaattgaa cctccaaatc
    21061 tttcctacta taccctagtg gtaaaaataa ttctagatta tccgtagcat aatgaacttc
    21121 ggaaatatta ttcgtataat cttttattat gatatgaagc aatacatctg gatgtttact
    21181 ctttactttt gcaatgttct ctcttgattt gaaaaaagcg ttaataaatg cctttttcgg
    21241 cacatgacgt ccctctgcaa tttcacgttc ttgtgtaaat ttccaagcga ttttagggtc
    21301 ttgataaaca tagaaaatcg tcactcgaaa atgttttttc aacgcccgcg agatattttt
    21361 ttcagcactt aatattgcaa aagttgcatc cagaataaaa gaatagccat gatctaagac
    21421 atatctaaat gcctgctcta ccaaccaaga agatgctttt tgaaagagac tagagttttt
    21481 tccattataa tcaggaaact gggtgcgaaa gtaatccgca tcaataatca catggttatc
    21541 ataattctca gctaatccaa tggcaacctc tgattttcca gccccaggac tccctgccat
    21601 aaaaactgcg tctcgtccaa cttcggggga tttccctttg ataatataag aaataaactt
    21661 atccttattt gcttttgcat actctaaata agaatcatta ctttccatat taattatccc
    21721 ctttcttcct actatataaa agtattatac gttacttgaa gtataaagtc agtattgaag
    21781 atcactcaat cattcatctt ttctaattta ctaattgaat ctaagaaatc ataccctttt
    21841 ggtacaagtg gcgtataaaa ctcactaatc acactgccac ctgtatccac ataacctcga
    21901 cctttgatgt gcttcataaa gaagtttttt tcgacctccc caaacatcat ggaatatccg
    21961 agttcactca tacgacccaa agccacgcga aagttgaatt gatcgcggat tccgtctcct
    22021 aaatatttcg catccggcct ttggcaagcc aaaatcagaa agaaaccaga ttgacgaccc
    22081 aacatcacga tttgtttgag cttattcaaa atcaccgcac tttctttcgt cgttaacatc
    22141 tccatataag ccacgtattc atcaaagatt aaaaagttgg gtggaagtcc aagataggca
    22201 taattctctc ctgttttgta gttagacatt tctttcattg ccttgctacg agccatcata
    22261 cgttcataga aatcttccac acaagcagaa atttcttcct tttgtgaata aacatgaggc
    22321 atcaccgtac ctaaatcagc taaatctgca tttttgggat cgaggataaa caattctgca
    22381 tctgacttca atagtgcttc gataatggtg aggaggaaat aggtcttccc tccacccgtg
    22441 ccaccagcga ttaacatatg gggtaaagaa tcataagccc atacttgatt tttcatcaat
    22501 cgcagagtcc cattttctgc cactacttcg tctatcccaa ttcgattggc gatcatatca
    22561 tacagtaaag tatactccac ataggagtct ttgagttcct tttctactaa ctcacaatac
    22621 agtccgcttt ctaacttctt ttccaatttt aaaagctgat cttggtattt cccaagtgaa
    22681 atttgaacgc gaatagacag taatccatct ttcattcgat aacagatttt ggggaagtaa
    22741 ctgatagtct ctctggttcg gctactattc aaatctttga aaaagccttc actttgagtt
    22801 tgtttcactt cataccaatg gttctctaga atcatccgag ctagcttttg tcgatgccac
    22861 aagcttcgcc aatgatcaag aaaaaagtga atataaagca atatcatagc tcctgaaaaa
    22921 gcgagacttc caagcagaca tactaaatct tttatagacc aaacgatttc aatagtagaa
    22981 agtgatagct tagaaatatc aaattgtcta aactcttgaa aataaaagac gctcatccaa
    23041 ccgatgaaca gagcgagtag ccagccaaga acaaagtgat aaaccaagtg ctgatctcct
    23101 gcgcgtatgc gatgtccttt gtacattttg atggtttcca cctccaatcg aaaaagccca
    23161 ataggatttc attaggcaat ttcaaaaagt tatgctagtc atttactgct acgaggaggg
    23221 gagttgtttc gtttcaacca attgatttaa aaagccttca atagcatccg gcatctccac
    23281 accaatttct tccaaaatgg attctgcctg ccgacataag ttttcattta cttcgacaac
    23341 tataaagaaa gactcttctt ctcctaacca gtctagtaca tcagagtctt cttcactgtc
    23401 gctattttct ccaagtaaac cttgatgtat aatcagaaac attttttcat cagaggttaa
    23461 tgctttaacg agttgttcct cagtcagctc gccttttcca tattgatcga gacaatctaa
    23521 aatatgttca acattaattt ccataatctt cacctatttc tttctttcat tttggttcgt
    23581 aggatttcct tgattcggct gccgcttcaa gactaaatca tccgccttaa tgaaccaatt
    23641 aacggtagtt ccttctcgaa aaacataatt ggcaacggta tccacgattg gattgaccaa
    23701 ctctacctct gcatcataag caaactcgcg taagggtaca ttagctggga tactaacttg
    23761 aatcattcgc ccttgtgcag aggattttaa attataggtt cgttctttta cctcattacc
    23821 aattgttcca tcctcatttt gcttccgtac ttctcggcgt aatgcggaaa aacgcaactt
    23881 cccaaatgtt aaacctgaat ctacgacaat accttctgtt aatctcatct tcttttcctc
    23941 ctatgctttc actaaatcat caacatacaa cacgtaatca gtaaacccac gattctcaat
    24001 ctcatagcct ttggcaacca aatggggatt cacggctttt agcggttggt cttgatcaaa
    24061 atccttttca ccggcttccg cagctactac cacctcaata tcatcggccc gttgcttact
    24121 ggaatacaaa tgataactgc gaccaatcac cgtagtattc cgtccatatc cttctgttat
    24181 aatttctcct tcaccggcat aacttaactt tccaaaggtt tcagccatat ctggcacgac
    24241 aaatttcaat tccatcttct tttcctcttt tcattgttag ttttggatac aaaaatagcg
    24301 cgactacttt tttaggaggc ggtcgcgcac gttggttctt aggatttact tgtatcatct
    24361 catcacctta gttccggcga ttccagaaat aatagcccgc cgtcgcaaaa atcaaagtaa
    24421 aaccaatgaa cagtaaaaca ttggaatttt tctccccggt ttgagggaag gtcttcactg
    24481 tgggactaga ttcttgagag ccacttggaa cagttggtgt ttccggttgt ttcggcgtac
    24541 ttagtatggt tggaacttct tttggtgtta aggtttgaga tttttctttc aaatcttcat
    24601 tgtgttttcc attgatattc ccatctttat cgtaattgat ttccgcaaaa gtaaacttgg
    24661 ttccttctgg atattttccg gtatctactt ttttcgcaag cacagttttg gtgaattctt
    24721 tatcatttac ttcataatca atcttgccag atttccaaat ttctttgttt gtaccatccg
    24781 gtaataaagc atacagaatt gtttcgaaag cttcttttga gccatccagt acatcatgag
    24841 taatcgacac atcatcaaac atatccacca catcaccatg agtaaaggtc tgagaaccgt
    24901 tttctaggtg ggcctttgtt tggatagaaa catggtgttc cactgtacaa ctgacagttt
    24961 gggcttggtt attcaagcta acatccttcg ctactggctt atcgcctgct tcaaaagctt
    25021 ccttattttc atacacatag ttgaataaga caatagtttt atttaaaact tgttcggcgg
    25081 ttaatttatg ctgaattttc caagttcctg ttttctcttt gttcgccaac gttgcgcttg
    25141 tttctgcaat caccactggt ttggcttttt catctttttc ttgggcagct tttgtcgcat
    25201 ccacatcaat ggcttgcgct accacatacc agtcttcttt gatttcatgt agattgtaac
    25261 tcaatttatc aactagctcg gtagaatcca gagaagttaa ttctttgttt ccgtctttcc
    25321 aagtcgcaag agaagttaaa ctatcttctt cctcaggcaa atcatagagc attaaacggt
    25381 tcaaactaac agaaaatgct ttgttcgtca gcttctcgta aggaacggtc accgttttaa
    25441 ttggttgttt ttggtcttgt tcggtaatgg taaagacata ctcactcttc acaaagtctt
    25501 ctttgttttc cttaaaagta gaacggattt ctaatggcgt aatcttttga aaaccttctg
    25561 gggcttctac ttcttcaagt aaataatccc cataaggaag attttcaaac ttgccataac
    25621 catcaaaccc taattgctcg ttataagctg tggtcgcttc atcttcggca cctgtgattt
    25681 cattggtccc ttccaatggc gacactttaa aggtcagatc attgaagcca gtttcggtag
    25741 tgcccgcagc tgatccagca aatttaaaga aatcaaagcc aaagcgaatc acttgttctt
    25801 ttgccgtaac atttcgggta atcacggcat ttttttcgct atcatccacc tttttgatgg
    25861 atacaggata ctttgtttca tcaaggctat atccttcggg cgcttttgtt tcttgccaat
    25921 aatactcgtt gatagctaaa tgtttaacgg caacttggtt cttttcgtct aaagtcaaag
    25981 tgaccgtttc atcagaacct ttcgttccct tcgctaactc tggtttaaaa gcctcactcc
    26041 atttaacagc ttttccatcc ttagcagtaa acaaggtgta ttcagcaccc tcaaagactg
    26101 ccttaccttg tgctttatcc ccagtatctt tgtcttcttt cgtcaaagtg gtttcgccag
    26161 tgacttcttg attttgccct tttacgttac tggtaacagg ggctacggtt tgattggcat
    26221 acttcaattc gacttttaca ggtttgaacg tattcacgaa cccatgacta gctttagttt
    26281 cggtcacata ataggttccc aattccaacg cattagcaat ctcttttggt gtttccgcat
    26341 gaccgttttc atccgtggtc atttcttgaa cgatttcacc ggttggactg tctttgcgaa
    26401 tggcaaacgt attaccagct aaggaataat tgtcattcca aagatcactc cctgtttcaa
    26461 ctccgctttt atccagaatg atttgccctt tttcccgtgc gttttttgaa gtcacgtaaa
    26521 tggtttcgcc tgctttaatg gtggtagtca tgggggtggt atcaatcgta taaggcgccg
    26581 gtactgattt ttcggtaatg gttacttttg taccatgagg aatctcatcc aatgtagcaa
    26641 tgccttcttt gtcagtagtc acgtcttttg caggtaaagt ttttccaaag tctaaatgga
    26701 aaaccgttcc tggtacaaca tcacccgatt ctttatcgac ttttttgatc tttaaagaac
    26761 cctccgtttc cacatcaatt ttgattgtgt aggtattagg tttatcaatc gccccggcca
    26821 ttaaagtctg ttggccagcc tttttatagg cgacaggtgt tccggtacca gcagatttct
    26881 taagcgtcag cacaccactt tcttttgaat tggcatttgg ggtgattatc aactgattac
    26941 cattcacacg ataatcaatg tttgcggcat tttccacaac ctcatcgaac tctgacaaat
    27001 tcagattatt cgtatccgtc acagtagtcg attgccctaa tactgtttta gccgtactat
    27061 tatggaaggt tggtttcttc tgataatcgt caatggcttg atttattttt gcctcaatgg
    27121 ctgcgatatt tacggcacta ccatctgatc gttttattga gtgaagggta tacccattaa
    27181 cttcttgcca gatcatcttc tgtgccacaa tatgagtatc tgcatctgta cctacttttt
    27241 tccataaaac ggaaactaat ttggctttat cggacatatc tggtaacggg tttttctcat
    27301 agccaggagt gaactcattg taaatcggga ctcctggttc aatacagaaa atatcttgtt
    27361 taaagccgtt gtacactgca taatgcggaa cagtgacttc tgacattgga cgaccatcac
    27421 taaaagttcc ttcaacagca tattgatgtg ctaaatctag ttctacggtt accgtttgtg
    27481 gatgggtaat ttcatcagca aaagtaatcg taggagcaat tgctccaaca attgcttgaa
    27541 cagccaaact caaaatagtt aatacacata atttcttttt ttgtttttta tacatgaaat
    27601 g
//
LOCUS       DAYYHS010000031        27529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000031 DAYYHS010000000
VERSION     DAYYHS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.031"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..660
                     /locus_tag="ACHA6A_001310"
     CDS             13..660
                     /locus_tag="ACHA6A_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8969517.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            744..1688
                     /locus_tag="ACHA6A_001311"
     CDS             744..1688
                     /locus_tag="ACHA6A_001311"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8969518.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1823..3274
                     /locus_tag="ACHA6A_001312"
     CDS             1823..3274
                     /locus_tag="ACHA6A_001312"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8969519.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3295..4131
                     /locus_tag="ACHA6A_001313"
     CDS             3295..4131
                     /locus_tag="ACHA6A_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8969520.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4131..4790
                     /locus_tag="ACHA6A_001314"
     CDS             4131..4790
                     /locus_tag="ACHA6A_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8969521.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4916..5605
                     /locus_tag="ACHA6A_001315"
     CDS             4916..5605
                     /locus_tag="ACHA6A_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969522.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5611..6567
                     /locus_tag="ACHA6A_001316"
     CDS             5611..6567
                     /locus_tag="ACHA6A_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8969523.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6611..7123
                     /locus_tag="ACHA6A_001317"
     CDS             6611..7123
                     /locus_tag="ACHA6A_001317"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8969524.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7379..7927)
                     /locus_tag="ACHA6A_001318"
     CDS             complement(7379..7927)
                     /locus_tag="ACHA6A_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8969525.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8016..9197)
                     /locus_tag="ACHA6A_001319"
     CDS             complement(8016..9197)
                     /locus_tag="ACHA6A_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8969526.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9433..10941)
                     /locus_tag="ACHA6A_001320"
     CDS             complement(9433..10941)
                     /locus_tag="ACHA6A_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8969527.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11100..11291
                     /locus_tag="ACHA6A_001321"
     CDS             11100..11291
                     /locus_tag="ACHA6A_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969528.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11494..12411
                     /locus_tag="ACHA6A_001322"
     CDS             11494..12411
                     /locus_tag="ACHA6A_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8969529.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12404..13477
                     /locus_tag="ACHA6A_001323"
     CDS             12404..13477
                     /locus_tag="ACHA6A_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969530.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13625..14143
                     /locus_tag="ACHA6A_001324"
     CDS             13625..14143
                     /locus_tag="ACHA6A_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969531.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14124..14516
                     /locus_tag="ACHA6A_001325"
     CDS             14124..14516
                     /locus_tag="ACHA6A_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8969532.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14592..15527
                     /locus_tag="ACHA6A_001326"
     CDS             14592..15527
                     /locus_tag="ACHA6A_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8969533.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15660..16439
                     /locus_tag="ACHA6A_001327"
     CDS             15660..16439
                     /locus_tag="ACHA6A_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8969534.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16432..16749
                     /locus_tag="ACHA6A_001328"
     CDS             16432..16749
                     /locus_tag="ACHA6A_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8969535.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16993..18603
                     /locus_tag="ACHA6A_001329"
     CDS             16993..18603
                     /locus_tag="ACHA6A_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8969536.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18760..19608
                     /locus_tag="ACHA6A_001330"
     CDS             18760..19608
                     /locus_tag="ACHA6A_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8969537.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19839..20318
                     /locus_tag="ACHA6A_001331"
     CDS             19839..20318
                     /locus_tag="ACHA6A_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8969538.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20351..22579
                     /locus_tag="ACHA6A_001332"
     CDS             20351..22579
                     /locus_tag="ACHA6A_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8969539.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22708..24351
                     /locus_tag="ACHA6A_001333"
     CDS             22708..24351
                     /locus_tag="ACHA6A_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8969540.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24424..24969)
                     /locus_tag="ACHA6A_001334"
     CDS             complement(24424..24969)
                     /locus_tag="ACHA6A_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8969541.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(24992..25477)
                     /locus_tag="ACHA6A_001335"
     CDS             complement(24992..25477)
                     /locus_tag="ACHA6A_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8969542.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25467..26279)
                     /locus_tag="ACHA6A_001336"
     CDS             complement(25467..26279)
                     /locus_tag="ACHA6A_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8969543.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26418..>27529)
                     /locus_tag="ACHA6A_001337"
     CDS             complement(26418..>27529)
                     /locus_tag="ACHA6A_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8969544.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9014 a         5019 c         5517 g         7979 t
ORIGIN      
        1 gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta
       61 gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa
      121 cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag
      181 acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg
      241 atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga attagatagt
      301 gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca
      361 agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat
      421 gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt
      481 gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat
      541 ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt
      601 acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa
      661 gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata
      721 acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg
      781 ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac
      841 tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag
      901 gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc
      961 caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa
     1021 accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa
     1081 tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc
     1141 ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat gaaacactga
     1201 aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt
     1261 ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg
     1321 ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca
     1381 aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga
     1441 atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga
     1501 ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg
     1561 gtaccgagct tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc
     1621 agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca
     1681 tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc
     1741 gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa
     1801 cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga
     1861 aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa
     1921 tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt
     1981 gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg
     2041 tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc
     2101 ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca
     2161 aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc
     2221 aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc
     2281 agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat
     2341 acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg
     2401 tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat
     2461 gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca
     2521 agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg
     2581 tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc
     2641 tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg
     2701 tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg
     2761 ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg
     2821 gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt
     2881 atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc
     2941 accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag
     3001 agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc
     3061 catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca
     3121 accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt
     3181 tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa
     3241 tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag
     3301 aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca
     3361 gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta
     3421 gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt
     3481 ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt
     3541 aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct
     3601 gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc
     3661 aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt
     3721 gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg
     3781 gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc
     3841 gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc
     3901 gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac
     3961 gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta
     4021 ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact
     4081 gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc
     4141 ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat
     4201 acacattagg gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg ttgcttttga
     4261 cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt
     4321 tctttagagg cattcccgca ctagtcctgc tgtttctgtt atattttggg ttaccttatc
     4381 aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg
     4441 gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag
     4501 cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa
     4561 tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag
     4621 caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg
     4681 attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg
     4741 aaaaagtgca acagtattta gaaaaacgtt ctatgcatca gtcaatctga aaaatagaaa
     4801 tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat
     4861 tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa
     4921 gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg
     4981 cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg
     5041 cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc
     5101 agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc
     5161 aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta
     5221 tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa
     5281 agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc
     5341 tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaac
     5401 agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga
     5461 acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga
     5521 agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg
     5581 gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa
     5641 tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa
     5701 ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa
     5761 aaaaagcacc atcgaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc
     5821 gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc
     5881 ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat
     5941 ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa
     6001 tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc
     6061 cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt
     6121 tttagtgatg actatctttt tttccacgca gacggatata ttggattttc tgatttcacg
     6181 atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta
     6241 acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac
     6301 tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc
     6361 atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac
     6421 cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg
     6481 gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt
     6541 caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag
     6601 aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat
     6661 cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca
     6721 gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat
     6781 agtggaagat caaattgctg gaacagctgc attagtctca agcgtggatc ccgtttacac
     6841 aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc
     6901 tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac
     6961 gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc
     7021 gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt
     7081 tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag
     7141 caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta
     7201 acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt
     7261 gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt
     7321 cgtgacatag cttttgtcac gacctctcgg cttatcctac gagtattatt cgatacattt
     7381 atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca
     7441 cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa
     7501 ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca
     7561 atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata
     7621 aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt
     7681 taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact
     7741 catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat
     7801 ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa
     7861 aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat
     7921 aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc
     7981 caaagctttt aaaagtaact ttgctttttt gtatattata ccagcaagcc agtcacaata
     8041 gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca
     8101 agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag
     8161 aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg
     8221 aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc
     8281 atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg
     8341 gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca
     8401 ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca
     8461 tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct
     8521 tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga
     8581 atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt
     8641 gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg
     8701 aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat
     8761 tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt
     8821 ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga
     8881 ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca
     8941 cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga
     9001 agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat
     9061 aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt
     9121 gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata
     9181 cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt
     9241 gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc
     9301 attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgccttagt
     9361 aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata
     9421 agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat
     9481 atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg
     9541 ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt
     9601 tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga
     9661 atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg
     9721 aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc
     9781 atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat
     9841 cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc
     9901 agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat
     9961 tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata
    10021 acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa
    10081 tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat
    10141 tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc
    10201 tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac
    10261 tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa
    10321 agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt
    10381 caaggaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt
    10441 agttgtttca atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt
    10501 gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt
    10561 attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat
    10621 taataagcca taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat
    10681 cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat
    10741 ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg
    10801 aagtacacgt ttcgcatgct tgtcgtcaac tagcttccca ccaaattcta aatataattt
    10861 gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga
    10921 atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt
    10981 tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa
    11041 accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca
    11101 tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg
    11161 gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg
    11221 aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc
    11281 ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa
    11341 gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat
    11401 tgcccgctct tttttataac aattttttac agaaaatggt actattgaag tattaataag
    11461 tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat
    11521 tatgagccag acgaactaga ggagacgaat gcggatcagg atacaggaca agaatcgtct
    11581 tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta
    11641 caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat
    11701 ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg
    11761 ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa
    11821 aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg
    11881 gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg
    11941 gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca
    12001 aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat
    12061 cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa
    12121 gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt
    12181 ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca
    12241 agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat
    12301 ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc
    12361 aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga
    12421 agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt
    12481 ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac
    12541 aaaaatacac gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca
    12601 tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga
    12661 tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga
    12721 tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga
    12781 agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact
    12841 gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac
    12901 aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt
    12961 attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc
    13021 agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc
    13081 agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt
    13141 attagcccat gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa
    13201 gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat
    13261 ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta
    13321 tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac
    13381 agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta
    13441 cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat
    13501 gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa
    13561 gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt
    13621 aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg
    13681 taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat
    13741 agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag
    13801 cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt
    13861 tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa
    13921 gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac
    13981 gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt
    14041 gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt
    14101 ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg
    14161 tcagtatact cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa
    14221 aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact
    14281 tttcggaaaa agaagtgttc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa
    14341 attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg
    14401 agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca
    14461 aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt
    14521 cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga
    14581 aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag
    14641 gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg
    14701 ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat
    14761 tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag
    14821 caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg
    14881 gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca
    14941 ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa
    15001 tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa
    15061 gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg
    15121 tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga
    15181 tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat
    15241 tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag
    15301 cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa
    15361 cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact
    15421 cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata
    15481 tatacattga aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat
    15541 gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta
    15601 gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa
    15661 tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta
    15721 taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc
    15781 ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg
    15841 catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag
    15901 aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga
    15961 cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa
    16021 agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa
    16081 tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag
    16141 atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga
    16201 tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca
    16261 tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg
    16321 gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta
    16381 aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa
    16441 ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc
    16501 ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat
    16561 gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat
    16621 ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac
    16681 aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct
    16741 atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg
    16801 atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa
    16861 aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat
    16921 ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt
    16981 gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat
    17041 cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac
    17101 aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg
    17161 ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc
    17221 acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg
    17281 tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga
    17341 atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct
    17401 ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg
    17461 cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct
    17521 ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt
    17581 acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc
    17641 agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa
    17701 aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca
    17761 gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc
    17821 cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca
    17881 aataaattga cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca
    17941 atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt
    18001 ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga
    18061 ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct
    18121 ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg
    18181 aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc
    18241 caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca
    18301 ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct
    18361 ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca
    18421 gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat
    18481 gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa
    18541 ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa
    18601 taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt
    18661 gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata
    18721 aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca
    18781 gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca
    18841 atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa
    18901 tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg
    18961 gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc
    19021 catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc
    19081 agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg
    19141 gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat
    19201 atcgatcgaa tccgagataa tacctacgct tatctgattg tagatgattt gaacaattta
    19261 gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa
    19321 ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa
    19381 aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca
    19441 gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa
    19501 ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt
    19561 acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc
    19621 aaaaaacata ccaaaaatat tccaacatca tcttggactt tctacatgtt actaatgagc
    19681 gtgtaaaaga acgagatggt gtttttattt ctatcaatga agctcttttt ctttgcaatc
    19741 ctcacttcaa tcctctataa tgtcgcatat agtttagtaa atgagtaaac tactaattta
    19801 cttatttact aaaaataaaa ggatgaggag ttaaaataat ggaaatacca acaaatttaa
    19861 aacataaacc agtaatcgtt gtagaagact atgataaagt tgatggaaga aatgcattaa
    19921 atacggatgc aaaagggctt tcactgggcc ttgctcagtg gaatgatcgt ggaaaagtcg
    19981 atatctcagg caaggtatgg cgacatactg gagaaaaatg gtcacgacag tcggaagaat
    20041 taccaatcac acgtatttta gatttagcga tcttagctgc acaaggaagt ttatacttcc
    20101 aagatgctta tcgttatgaa aaattttatg atccagaaaa tccagtcgta gacatcattg
    20161 gattacaagg gaaccgaatg acggtagaag tagataccaa aaatccaaag atcgatgaag
    20221 acatcatttt gtattacgat acactacaaa aagacggaga actgattggg gaacgattcc
    20281 gtattttaaa acgtctttta gatgagctag gatattaata gacagagatg atcaaaaggg
    20341 gaagagaagc atggagaatt tctcactaac agaaaaccga accttttcac ggctagaaaa
    20401 gcaaatgatc tggaagaaac cgaaaagcca tcggacaagt gcagaagaat ggcggatcgc
    20461 ctccgaaatc aaagggaatt ggcaagatcc tgaaatgaaa atcagtaacg tacttttgga
    20521 aggagatgcc gggtcgggga agacacaatt agccaaagca ctatctgctg atctgcaact
    20581 tccctataca aaagtgacct gttttgcaga tatggacaaa tcagatgttt tcggagcctt
    20641 attacctgta gtagattcta ataaggaaca agaccaagaa ttactagaag ctatttacca
    20701 aacagataca cttgaagctg tactgacact gatccagaac cattacggca ttgatccttt
    20761 ttctgctaaa gaaaaattag cagagctagt tcaaaggatc gaagctgatt atccagatgc
    20821 aatccattac agctattacc cttctgaaat cgttcgagcg attgaaaaag gatatttatt
    20881 agagattcaa gagccgactg tcatccgaga tgcctctgtt cttgtggcac ttaactcagc
    20941 gctcgaacca aatgggatgt tgaatttacc gacaggaatc attcgtcgcc atccagattg
    21001 cgtcattgtg atcactacaa atcgaaatta ccaaggaaat cgcccattaa atgaatcttt
    21061 aagagatcgg atgcaacatg cggaaaaaat ggatcttcct gccttagaag tcatggcaga
    21121 acgtgcgatg gcgaaaacta tgatccaaaa acaagaactt ctcttaaaga tggcagagat
    21181 tatccgtttg ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag caggaatgcg
    21241 ttcttatttt tattgggcaa atacctataa gcaaggacaa gatttactcc agtcgattta
    21301 tccaaaagtt ttgtataaat tatccactga ttcagatgaa ttggcaattc ttgaacaagc
    21361 tttgtctgac agtggattgc tactggaact tcaggacata ttgagaaaag aaaaatgggg
    21421 tcatcctcaa tctgagcata caaaaggaag aaccattagc gccgaagaag caaatgaacg
    21481 aaacatcact gaaaatgctg aagaaattct gcgcttagat gaaaagaaat cgttgtctgc
    21541 tgaagaacca aatgagggag aagagaaaca agaagaaaca atagaaaaaa acgagattcc
    21601 tcctcaaatg caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa aagaacaaca
    21661 aacaggagat gattctcaag aaatgtctgc ttctgacatg gaagcacatg ataaggaaat
    21721 caagaagcaa ctgaataaag aagcgcgaca aatcatgaag gaaacgattc atgaaaaaga
    21781 aggtctcatc gttcaccgac cagctttcca aggaaaaacg ccggaagcac gtgagctaca
    21841 catgcaagta atgccaatag tagaatcact tagccggcaa attttagagt tactggataa
    21901 tgaacaaaca gagacgtacc aaaaaggaaa atacgaaggt cagcggttca atgcaagcaa
    21961 agtagcatat ggtgatttga gaaattttga caaaaagaac ccgccacatg agcaaccttc
    22021 tttagcggta gctgtacgga tcgatgaatc tgggtcaatg atacgggatg atcggatcga
    22081 agcggcaaaa aaagcagcga ttgccattgc cgaattttct aagaaagtcg atatcccatt
    22141 gttgatttac ggagatacag cagaccgttc gtcaagagaa aagacttctt tgttttctta
    22201 taaagaattt gaagacggat ttcaatggtt aaatgaaaaa ttagtcacta tgaaaccaag
    22261 gcaaaacaat cgtgatggtg ctgcacttcg tttagcagca gaaaaattaa acagacaatc
    22321 cgcaacaacc aaactgttgc ttaatattag tgacggtcag ccaaaagcgt tgccggatta
    22381 tacaggcaaa aaagcaaaag aagatatcca gtctgttttg aaagaatatg aacgtcaagg
    22441 aatcctattt gtctccgcag ctattggcca agacaaagaa gaaatcaagt cgatctacgg
    22501 tgaaagtcgt tttgtagata ttactgactt gaatgaattt cccaagcaaa tgattcaact
    22561 gattgcccgc tatctatgat aaaataaagc tcaacgaacg cacttagcag attcgttgag
    22621 cttttcgttt ctagttaccg gtaacaagga tacttctaaa atagaatcgg ttataatcaa
    22681 gagggaccgt taaaggagtg aaacgatatg atacaagaac aaaaatggtg gaaagaagca
    22741 gtaggctatc aaatctatcc tgctagtttc aaagatagca atggcgatgg tcaaggagac
    22801 atcaatggga tccgtgaaaa acttaattat ttgaaagatc ttggtatcgg ctttatctgg
    22861 atcaatccga tctatcaatc tccatttgtc gataatggtt atgacgtaag tgactatcaa
    22921 gatatagaag agagattcgg aacgatggaa gattttgatt tattgctaaa agaagcacat
    22981 gaattaggga tcaaagtgat tatggatctt gtcatcaacc atagttcgga tcagcatcaa
    23041 tggtttgaag aatcgaaaaa atctaaagaa agcccgtatc gcgattatta catttgggtc
    23101 gacggggtag atggcaaaga accgaataat tggacatcga ttttcggcgg ttctgcttgg
    23161 gagtattctc atgaaactgg acagtattat ctgcatgttt ttgcaaaaga acaaccagac
    23221 ttaaattggg aaagcgagaa attgaaagaa gaactattca atatgatccg atggtggttg
    23281 gataaaggga tagatggatt tcgtttagat gcgatttctc atgtaaaaaa agatgaatat
    23341 tcagtaaaag ccacagagaa ccctttctca ccctttcaaa atgtctcagg aatcgaagaa
    23401 catttgaccg atttgaaaca tgtttttgaa gagtacgata tcatgactgt cggagaagca
    23461 agtggtgtca ctgcagaaga aggaccacaa tgggtaggga aagacggtta tttcgatatg
    23521 atattcgaat ttgatcatat ccatatctgg cagcaagaaa aagaagggca attagatgtc
    23581 cttaaattga aacttgcatt aagcgcttgg caaacttcat tggatggtat cggatggaac
    23641 gctttatata tggaaaacca tgatgttcct cgtgccgttt cagtgtttgg agatactcgt
    23701 cccgattttt gggctatgtc tgcaaaagca atcgctatga tgtatttctt tttgcaaggt
    23761 acgccattta tttatcaagg acaggaaatc ggaatgacga atatgccctt tgaatcgatc
    23821 gatcaagttg atgcagtaga ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag
    23881 acaagagaag aagcattgga tatcattcgt gaaacaacga gagacaacag caggacaccc
    23941 atgcaatgga cttcggaaca atatgctggt ttttctacac atgaaccttg gctgatcact
    24001 aatccaaata ccaaaacgat caatgtagaa caacaagaat atgaaccaga gtcggtcctg
    24061 caattctata agaacatgat ccgcattaga caaaccaata aaggtctgat atacggttct
    24121 tataaagaat atcttcatga acatccacaa ctttatgtct atgaaagata tttagaagat
    24181 gaacaatatc ttattatggt aaacttaaca gaatcacttg ctgactatga acttccaaaa
    24241 gaagcagatc agtcctggac attactcttg tcaaattcct ctagcggaga atttgaagct
    24301 aaaggcatac tagcacctta tgaagctcgg ttatataaaa caaacaagta aaacttcctc
    24361 gttaaagttc attagttgga ctttgcgagg aagtttttca gattgctctg tcaattactc
    24421 atttcaataa ttcgctctag gaccaccgat atatttagga cgagagcgaa gggctgtcac
    24481 gtgagcttgt ccaagggttt tcaaacgagg tctgacaggt acttggacat gtttgatatg
    24541 cataccgatt gatgtgtcac cgatatcgat accggcttgt ccaactacgt gttccacttc
    24601 gacaggatca ttgaactggt caaaagcagc aacagaacaa gcacctccag catgtagtgc
    24661 aggaacaaca gagacaattt cgaaattttt tgcttctgcc actgttcgtt ctactactaa
    24721 tgctcggttc aaatgttcac aaccttggac agctagcgca atttctttag gatccaacaa
    24781 ctcttttaac gtacggatga tccattgacc aacttccgca ctggaatttt ttccaatatg
    24841 tcctccaacg acttcgcttg tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg
    24901 tgcctcagcc aatatgtcat taactatttc agtcaattgg tcttttaatt ctttttctga
    24961 caacttcatt ttctaaaacc ttctttttca attaatattg cttccgtgct tgaatacgtt
    25021 gaagacttgc agttatagga aaggcaagtc ccattccaat cagattttgg aagatgtttc
    25081 ctggtataga agccactcca gccggccaat catataaaat ccaaccggcg ataccatagc
    25141 cgatgatcat aactaagctt cctaataaaa gccctaatcc ccacatacga gtagaagatt
    25201 tgctgaaaga accagcaaca tatccttgta acccgtggat cactaatgaa aaaactgccc
    25261 actgcggata tcctgaaatc aaatcgatca atagaccact ggaagcacca acaactaatc
    25321 cacccgttgg accataaaga aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa
    25381 atccattcgt agccggtaca gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa
    25441 tcagcatagc atagatcgtt aattgcttag ttttcatttc tcattcctcc tttttgaata
    25501 ccgccagacc agacacttcc ggtatgatcg ttcatcagca cgccattaga aatacaatgg
    25561 gcaacaaatt ttttactttc tgctactgct ttggtaaaat ctgcccctaa cacaagattc
    25621 gctgttattg cagaagaaaa ggcacaaccg gccccgttta ctgttgtagt gtcgattttt
    25681 ttatgagaaa atacctcata atgatcaatt gaatagagaa gatccacagc ttcatctcca
    25741 gccatccttg tgccgccttt gatcacaatg ttttttgttc ctgaagcagt gagttcttta
    25801 gcgagatgga tcatttctgt taacgaagag ggaagagctt gaccagaaag taaagcagct
    25861 tctgccaaat taggcgtaat catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa
    25921 atatacaatt gattgatcac agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg
    25981 attgggatat tttgttttgt ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa
    26041 ttcaataacc caattttgat accatctaat gaagtcattt tatcaatcga aactaactgt
    26101 cgtttaagca attccaaaga tatagattcc agcataaatc gctcattaat atctagtgcc
    26161 cctatagaag tcaaggcgct tagtccgaat agaccaaaat tctcaaaagt ttttaaatct
    26221 gtttggattc ctccaccagc aaatggatca gaaccgccaa ttgtcaagat tcttttcata
    26281 acattttcct ccatgaataa gagtataacg aaattttcaa aaaagaaaaa cagccaattg
    26341 gcatattttt atctcatcca atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc
    26401 tttctttcct taaatagtta ggacttttgt gattttaatt ctttctcgat ttgaagccgt
    26461 tgcactttca ttgtagctgt tcgcgggatt gcatcaaaat cacggatgat agggacgttc
    26521 aagcgaggaa gttcatggac ttgctcccac catcgattcc aatccatttc tttatcagga
    26581 gctaaagcaa taatcggttg tggagaatga tttttatccc ttacaatcac tacttctgcc
    26641 aaaaaatcta acgaatctaa taaaaagtct tcaatagcta atgtactatt gatcgtttcg
    26701 atcaaatcca cttgccgatc tttcaaaaat aggtggccat gcttatccat aaaaccgtaa
    26761 tctccgctat cccaccaatc gccataaaca ttttcctgga accgtgcatc ttccttgtaa
    26821 taagtcaaag cccgtccttt agacagtaat tgtatatgac catctgtgtt ttcaggtagg
    26881 acattcccaa ctgaatctgt aatccgagcg cttgtcatgt cttccagtcc aacgcccata
    26941 tctcttgcat cacttgtttt caaactctct agtgtatgag ccttaagaat cattggtccg
    27001 cattcacttt ggccatatac ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa
    27061 aatgtcttca ttgtctggtt gtttatagca tcaaaagtgg agtgatagta tcggataccc
    27121 gaaaaagctt ctggatgctc cttagcagta aaacgccact gaacaaagtt attcggatgg
    27181 gtctctagtg caattggccg gtgagcttcc aacatatgaa caacttttga actttgtgcg
    27241 tttgctaaag gcatcatagg aaatcccatg gacatcaaag aagagatacc aatattgaat
    27301 ctggaatgca ctggagagat gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa
    27361 actgtttttt gccatttcgt ccgccatccc atagaatgag cagaatgaca aataagttta
    27421 gggataccag ttgtaccaga agtatgggtc atataagaaa tttcattttt gtctaaaaat
    27481 gattgagaaa cagacaaagc aggttgttcc attaaataaa gaataggaa
//
LOCUS       DAYYHS010000032        27451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000032 DAYYHS010000000
VERSION     DAYYHS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.032"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(238..600)
                     /locus_tag="ACHA6A_001338"
     CDS             complement(238..600)
                     /locus_tag="ACHA6A_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969545.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(674..1054)
                     /locus_tag="ACHA6A_001339"
     CDS             complement(674..1054)
                     /locus_tag="ACHA6A_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8969546.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1136..2311
                     /locus_tag="ACHA6A_001340"
     CDS             1136..2311
                     /locus_tag="ACHA6A_001340"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8969547.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2388..2972)
                     /locus_tag="ACHA6A_001341"
     CDS             complement(2388..2972)
                     /locus_tag="ACHA6A_001341"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8969548.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3173..4171
                     /locus_tag="ACHA6A_001342"
     CDS             3173..4171
                     /locus_tag="ACHA6A_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8969549.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4283..4792
                     /locus_tag="ACHA6A_001343"
     CDS             4283..4792
                     /locus_tag="ACHA6A_001343"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8969550.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4822..5481)
                     /locus_tag="ACHA6A_001344"
     CDS             complement(4822..5481)
                     /locus_tag="ACHA6A_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8969551.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5483..6247)
                     /locus_tag="ACHA6A_001345"
     CDS             complement(5483..6247)
                     /locus_tag="ACHA6A_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8969552.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6255..6932)
                     /locus_tag="ACHA6A_001346"
     CDS             complement(6255..6932)
                     /locus_tag="ACHA6A_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8969553.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6969..8354)
                     /locus_tag="ACHA6A_001347"
     CDS             complement(6969..8354)
                     /locus_tag="ACHA6A_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8969554.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8564..8648)
                     /gene="ffs"
                     /locus_tag="ACHA6A_001348"
     ncRNA           complement(8564..8648)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA6A_001348"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8671..9186)
                     /gene="tadA"
                     /locus_tag="ACHA6A_001349"
     CDS             complement(8671..9186)
                     /gene="tadA"
                     /locus_tag="ACHA6A_001349"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8969555.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9292..10149
                     /locus_tag="ACHA6A_001350"
     CDS             9292..10149
                     /locus_tag="ACHA6A_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969556.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10230..10565)
                     /locus_tag="ACHA6A_001351"
     CDS             complement(10230..10565)
                     /locus_tag="ACHA6A_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969557.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10632..12002)
                     /locus_tag="ACHA6A_001352"
     CDS             complement(10632..12002)
                     /locus_tag="ACHA6A_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8969558.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12080..12616)
                     /locus_tag="ACHA6A_001353"
     CDS             complement(12080..12616)
                     /locus_tag="ACHA6A_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8969559.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12925..14406
                     /locus_tag="ACHA6A_001354"
     CDS             12925..14406
                     /locus_tag="ACHA6A_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969560.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14509..15216)
                     /locus_tag="ACHA6A_001355"
     CDS             complement(14509..15216)
                     /locus_tag="ACHA6A_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8969561.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15295..15633)
                     /locus_tag="ACHA6A_001356"
     CDS             complement(15295..15633)
                     /locus_tag="ACHA6A_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8969562.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15744..16706)
                     /locus_tag="ACHA6A_001357"
     CDS             complement(15744..16706)
                     /locus_tag="ACHA6A_001357"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8969563.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16721..18052)
                     /locus_tag="ACHA6A_001358"
     CDS             complement(16721..18052)
                     /locus_tag="ACHA6A_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8969564.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18060..18740)
                     /locus_tag="ACHA6A_001359"
     CDS             complement(18060..18740)
                     /locus_tag="ACHA6A_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8969565.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(18959..19438)
                     /locus_tag="ACHA6A_001360"
     CDS             complement(18959..19438)
                     /locus_tag="ACHA6A_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8969566.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19565..21244)
                     /locus_tag="ACHA6A_001361"
     CDS             complement(19565..21244)
                     /locus_tag="ACHA6A_001361"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8969567.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21792..23294
                     /locus_tag="ACHA6A_001362"
     CDS             21792..23294
                     /locus_tag="ACHA6A_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969568.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23307..23969
                     /locus_tag="ACHA6A_001363"
     CDS             23307..23969
                     /locus_tag="ACHA6A_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969569.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24070..24273)
                     /locus_tag="ACHA6A_001364"
     CDS             complement(24070..24273)
                     /locus_tag="ACHA6A_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8969570.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24384..25568)
                     /gene="iadA"
                     /locus_tag="ACHA6A_001365"
     CDS             complement(24384..25568)
                     /gene="iadA"
                     /locus_tag="ACHA6A_001365"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8969571.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25725..>27451)
                     /gene="yfcC"
                     /locus_tag="ACHA6A_001366"
     CDS             complement(25725..>27451)
                     /gene="yfcC"
                     /locus_tag="ACHA6A_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8969572.1"
                     /translation="SSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGKFSTQD
                     IEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPEKGLEK
                     VIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGGFGFLN
                     FVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLERLALP
                     LLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNATSWMS
                     PFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLTYKSDD
                     YFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFFVMGLV
                     AGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASVPSALN
                     TILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADLVGVSR
                     QVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLGTIFIM
                     IAIAIGLQ"
BASE COUNT         8164 a         5745 c         4658 g         8884 t
ORIGIN      
        1 gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca ttactttgtg
       61 aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac atattttgaa
      121 aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt ttggaccaaa
      181 caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat ctgtatttta
      241 gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt gacctggttc
      301 ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag tttcaaagac
      361 aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca aactaatctt
      421 gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta aaccagtctt
      481 tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat ggaccaaaaa
      541 ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt tgcttgccaa
      601 aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca cattttcaga
      661 aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat gcttcttcta
      721 tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt ttattcgtga
      781 aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt cttaaagata
      841 aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact ggctgtccga
      901 cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa acatgaataa
      961 gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc cctgtcactt
     1021 ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc gcatttatca
     1081 ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc ttaagatgat
     1141 cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg acgcaattgc
     1201 tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg acttcttgtc
     1261 tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct ttggttatgc
     1321 cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac accatgctgt
     1381 ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt tagctgcagc
     1441 agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag tttatcctcc
     1501 attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt tgctagaaaa
     1561 aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag aaaacaatgt
     1621 aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga gcaaagaaga
     1681 attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta gtgacgagat
     1741 ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga ttgatcctaa
     1801 tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc tagctgcaat
     1861 caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg aaaaacattt
     1921 agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc aagcggctta
     1981 tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa atgttcaaac
     2041 agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc ctgaaggaac
     2101 ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat tggagaacac
     2161 tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc cttccggtca
     2221 tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag gacttaatcg
     2281 aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa aggacgagac
     2341 cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta ttctgaaatt
     2401 tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg ttgtacattt
     2461 gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt atgtcggaaa
     2521 tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg gaatccagca
     2581 ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat aaagaattgg
     2641 cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag gttgaacagt
     2701 tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc cattcctgtt
     2761 ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa aatcacgcca
     2821 tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg tgctagttca
     2881 gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt tggtcctttt
     2941 tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat ttgcatactt
     3001 cataataata ataacataat acctaacctc tcgtaaaaat actagatttg ttttctttta
     3061 tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc aaacattttt
     3121 gagaagcatt ctcaattaaa ttctgatata gtgtgaaaag ggaggaattt ttatggaaat
     3181 ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt tttacgcagg
     3241 aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg gtcaattagc
     3301 aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa taaaagcaag
     3361 tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat tccatttaaa
     3421 agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta caaataaagg
     3481 catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc agccaagaaa
     3541 attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt tcgtgaatga
     3601 tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt ctgctatcga
     3661 ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc gtcgcccgga
     3721 atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca acttgcttac
     3781 tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata tccggttaaa
     3841 aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg tcaattatgg
     3901 attctcttct aatttagaac atctttcttc ttggggactt gatagtacca gaaatgccat
     3961 tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg gtgacatctg
     4021 tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac ctactgcagt
     4081 gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc acagcacaag
     4141 cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca tttcaagtta
     4201 aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat agagaaagaa
     4261 aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag aaaaggcaag
     4321 agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg tcatgttttt
     4381 acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg tcaacagcgt
     4441 gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag atatattagc
     4501 agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg aaggccttta
     4561 cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga tcggctttac
     4621 gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa acagccacga
     4681 tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg ctgctgctgc
     4741 cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat aattcaacaa
     4801 aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt tcaagctatt
     4861 ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa ataagataaa
     4921 gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa cgggatcgaa
     4981 taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc gatcaaatcc
     5041 tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa acggacgaat
     5101 caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac tcgggatcaa
     5161 agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat aaaagtgatg
     5221 ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga agggattatt
     5281 gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta aagaagtata
     5341 atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt tgatcgtcac
     5401 accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat attccatcca
     5461 ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt ttcacatagg
     5521 tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat aaaacgagca
     5581 aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta tctccaacca
     5641 tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata atgatggctt
     5701 ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa gagatgactg
     5761 accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg ttcgttccaa
     5821 tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca taggtcagat
     5881 aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta aatccggcag
     5941 ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc ccttttccat
     6001 ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct ggagctacat
     6061 gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta gtcgtgttat
     6121 tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat cgtttaccgg
     6181 ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa tatcctttat
     6241 agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt tgttttttct
     6301 acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga ctctgttttt
     6361 cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt ctcatcgata
     6421 tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc ttttttaccg
     6481 ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta aagatagtct
     6541 tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg gaaaaatcct
     6601 tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg ggctagtaca
     6661 tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc ggacacaagc
     6721 cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac tagctcgcct
     6781 ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt cagttcttcg
     6841 gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt tttatgctca
     6901 acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt ttttattata
     6961 ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac acttcggata
     7021 aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa aaatggaaca
     7081 aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat tggcttgttc
     7141 ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac catctgccca
     7201 aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa gttacgattt
     7261 ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac atttaacaag
     7321 tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc ttttggaatt
     7381 tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg ttctttcacc
     7441 gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact cagagaaaat
     7501 ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag acaactattt
     7561 atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc tttggctgaa
     7621 tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac atactgcccg
     7681 tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct gaataaatga
     7741 tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat ttggaaatca
     7801 tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg taaatcttcg
     7861 actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg attataagta
     7921 agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt cgttgtaatg
     7981 attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa taagttatcc
     8041 aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt ggaatttcct
     8101 acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat cagcaagata
     8161 ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa atcacctaga
     8221 tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg ttgatccaaa
     8281 aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt atgtaaaatc
     8341 actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat aggcagcttt
     8401 ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca aattgtataa
     8461 gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt ttacagtact
     8521 ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat cagacacatg
     8581 gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag cccaccattg
     8641 tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc agtctttttt
     8701 taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca tcccacattc
     8761 gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt cctccaaaag
     8821 attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat aaacttctgc
     8881 tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg taacaaaaag
     8941 tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga ttgcaagcat
     9001 ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc ttccaatcac
     9061 ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt ctgctttctt
     9121 tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa gatttgtatt
     9181 taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata tgataaaaag
     9241 gctgtaacta actttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg
     9301 gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg
     9361 gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct
     9421 caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac
     9481 ccatggtatc actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg
     9541 atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt
     9601 ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta
     9661 gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct
     9721 ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc
     9781 accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat
     9841 ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa
     9901 gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa
     9961 atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa
    10021 acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct
    10081 ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa
    10141 cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg
    10201 aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc
    10261 cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt
    10321 tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga
    10381 ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt
    10441 actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact
    10501 gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt
    10561 ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg
    10621 aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt
    10681 acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc
    10741 ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc
    10801 aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat
    10861 cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga
    10921 gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta
    10981 gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt
    11041 tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa
    11101 gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt
    11161 ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa
    11221 ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt
    11281 tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt
    11341 attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc
    11401 cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca
    11461 accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt
    11521 gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt
    11581 ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg
    11641 gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca
    11701 gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg
    11761 aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt
    11821 aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac
    11881 cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg
    11941 cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc
    12001 attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc
    12061 tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat
    12121 ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat
    12181 catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt
    12241 tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt
    12301 ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca
    12361 ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat
    12421 aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat
    12481 aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca
    12541 ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg
    12601 ttactctccc catcattctt acctccgtct cttttcatct acccataaat aaataacata
    12661 aataattcta aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa
    12721 tcaatttttc cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat
    12781 agaataaaca aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct
    12841 gtgagaataa cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa
    12901 aaacagaata aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt
    12961 ctacaaatgg tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat
    13021 attccaaaac ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt
    13081 ttattgattg atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc
    13141 gatcaaacta aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc
    13201 aatcgtttta actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa
    13261 tatttacttg aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc
    13321 aatcattcct taataaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt
    13381 gaaggaaatg agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga
    13441 ggtaacgatt ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat
    13501 gagcaaatag aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc
    13561 atattacgaa acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg
    13621 atggacttat tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt
    13681 cgcccttgtg aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt
    13741 tttccaaatc aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc
    13801 aaaaacctta tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct
    13861 ctcaaccatt tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt
    13921 tttttcgaac tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc
    13981 acgtccaatc attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca
    14041 attttttcaa aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa
    14101 cacttactac aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag
    14161 tttacaggaa attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt
    14221 aaaattaccg catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt
    14281 tcagatgaaa cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag
    14341 ttaataaaag cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg
    14401 atgtgacaaa attcttgtca catcctctta ttccaaataa atggtattca atcagctaac
    14461 tctcggtatt agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg
    14521 gttgccgccg aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc
    14581 aacataagtc agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt
    14641 cagtagtccg ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg
    14701 taatgtcacc aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa
    14761 cgtagagttc cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc
    14821 aaagttagct gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc
    14881 ttgtaccgca tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg
    14941 tgcagttgct tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc
    15001 tccggcaatt tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc
    15061 tgcttgtgta ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact
    15121 attgacgtac gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt
    15181 cggatctaaa aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta
    15241 ctttatttta tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt
    15301 ctttttcaca aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat
    15361 attatgatcg ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc
    15421 tgacgcacct tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg
    15481 gtttccattt gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt
    15541 gttccattcg ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc
    15601 tgagccacat tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg
    15661 agcactattg taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa
    15721 aaggcacgtt ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata
    15781 tcttggatct ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt
    15841 gctaatggat ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta
    15901 gaacgtaaac gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc
    15961 acttcgtcaa tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca
    16021 tatttatcaa gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg
    16081 atattttgat aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct
    16141 ctgttcaaag cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta
    16201 cgggacgtcg tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt
    16261 aaccgtgcag ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca
    16321 atcatctcac tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca
    16381 tcattgggat gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg
    16441 attctgggtg aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct
    16501 tcaacttctt gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt
    16561 atttcttttt ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat
    16621 gcatcaggat cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta
    16681 tctactattt cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt
    16741 gacaaacaac gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt
    16801 tagcggaacg tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt
    16861 ggatcagccg caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca
    16921 tcaccattgt tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg
    16981 ttccaacgcc attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac
    17041 gatttccttc acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg
    17101 tatctgcaat ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg
    17161 taatcaagcc ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc
    17221 agatcatttg atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt
    17281 tcgtcatcac ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa
    17341 caatccccat caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt
    17401 ctgacaaacg ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag
    17461 ctggcatata gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg
    17521 ctcggttgat catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt
    17581 caagatcatc agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata
    17641 ctgcggcgcc atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta
    17701 gtgatcctgg cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt
    17761 tattcaagac cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga
    17821 cgacctcgcc aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg
    17881 tccctactcg ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt
    17941 acgctgttcc ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc
    18001 caacaggtaa actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc
    18061 tacacttcca gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct
    18121 acaaaagatt cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact
    18181 ttcttggcac gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta
    18241 tcgccaatcg gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca
    18301 ctgaaataag cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt
    18361 ccgctaggct cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt
    18421 gcatgtacga attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg
    18481 aatgaatcta ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg
    18541 gcttgatttt cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta
    18601 atcagcaacc aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta
    18661 ataaacgggt caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt
    18721 ccatgatacg taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa
    18781 aacgatttca tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg
    18841 attgatagta cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc
    18901 aagcagatat aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt
    18961 aactaattaa atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc
    19021 gaaggcttct cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac
    19081 tgtctgctaa aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc
    19141 cgtggggata tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat
    19201 ctttcgtcac ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga
    19261 tgatcacccc agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa
    19321 tccctctttt ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg
    19381 gttgatagct gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg
    19441 attcactccc tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat
    19501 ttgtttttcg caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt
    19561 ttgtttattt tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc
    19621 ggcgttttgt ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt
    19681 tgctccactc aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc
    19741 tgctttcttt catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt
    19801 gaggttttga aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc
    19861 cgtcttctga caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta
    19921 atccatcaat cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct
    19981 tatattccag cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg
    20041 catgagccaa atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat
    20101 atgttggttt catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa
    20161 cgcgcatatt ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg
    20221 ttttcgccac gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc
    20281 cttcttcaat cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg
    20341 gttcacccat tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt
    20401 ctttcaacgt aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg
    20461 tgcgaataat tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg
    20521 ctgcatccaa tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac
    20581 gcccttccca atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta
    20641 caggctgagg attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc
    20701 cgatttccgc caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt
    20761 taaaatctcc cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat
    20821 ctggtatcgt atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg
    20881 tatgctcatc taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat
    20941 tcaattttgc taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca
    21001 acaagtaagg aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta
    21061 cacgatcaat gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca
    21121 actcattttc aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg
    21181 cgacatacat gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac
    21241 tcacaaactt cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat
    21301 ctaaatatat gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga
    21361 gaatcagcag gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt
    21421 tggatatact tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata
    21481 taagtattca gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca
    21541 attgttagaa caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta
    21601 ttcctctttc atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt
    21661 ttgcaataac tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca
    21721 gcataagatt taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag
    21781 ggatgcaaaa tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac
    21841 gttcattgtt attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca
    21901 cgattcgaag cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag
    21961 tgactttaag tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg
    22021 gttctaccac tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa
    22081 cggacgcgga aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg
    22141 cctcagctat ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca
    22201 gttcagaaac tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa
    22261 tgactcaagc agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt
    22321 ctgatggaac agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa
    22381 acaatgtagt cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt
    22441 tcacaatcac gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt
    22501 atgaatccag cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga
    22561 atacacttta cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca
    22621 cgatagattc tgctatttac actctatctg atccagataa aatggatgag tttctcaaag
    22681 aagctgagaa gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc
    22741 agtcaatgtt ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag
    22801 tggctgtagc cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc
    22861 ggcatgagtt aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat
    22921 tgtttaccga agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa
    22981 acgtcgtcgc gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac
    23041 aaaaccagca agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc
    23101 aacgcgatgg tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg
    23161 aattggaaat caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa
    23221 tcagtctgtt ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa
    23281 tcctattaaa ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc
    23341 aagatccgca agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt
    23401 acgctatttt aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac
    23461 tagatgcacc aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt
    23521 taagaagtta tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt
    23581 atttatcggc tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga
    23641 attatcgaca agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa
    23701 aaaaacaggt gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta
    23761 tctgctgtga acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa
    23821 cttcacaaga tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta
    23881 tcatcgtgac tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa
    23941 ataaaaaatt tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg
    24001 aaccatatct gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat
    24061 aagtggtttt tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa
    24121 atttcgagct ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt
    24181 gctcttgtcg tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt
    24241 catatcttta gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat
    24301 tgatctacct tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta
    24361 aaaaagatag ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact
    24421 tctttttctt ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct
    24481 ttatccaaga tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat
    24541 ttcaatatac gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt
    24601 tctttttgca cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac
    24661 cctaggaaat gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc
    24721 gtgaaattat ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga
    24781 acttcaccat ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg
    24841 ccaccacgtt tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga
    24901 gtaggcaaaa attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct
    24961 agctttcttt ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg
    25021 cctcctacac gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa
    25081 cggtgatcgg atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct
    25141 ttgatgatag aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa
    25201 gtggagattc cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta
    25261 ccttctccgt ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg
    25321 ctcaatgtga cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata
    25381 tggaaatgac agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct
    25441 acttccactc cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg
    25501 gcaatcagta catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg
    25561 attttcatac atttttcctc tttctttaag tataactgat gataggatct taaaaaataa
    25621 tccaaacgat aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc
    25681 ttatctactg tttggattac ttttttattt acaggtctcc agtactactg cagacctatt
    25741 gcaatggcaa tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt
    25801 tggaatcttg cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg
    25861 gctgatacgg gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct
    25921 acttgtctgg atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct
    25981 gcttgtcctg aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc
    26041 caagcaccaa tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta
    26101 ttcaaagctg aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc
    26161 atacctacta cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg
    26221 atgtcattga ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat
    26281 cccatcacaa agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc
    26341 caaacgatcc agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg
    26401 aattttttat tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg
    26461 taagtcaacg aactttctgg ttttttacgg attttttctg catatatcat catataacca
    26521 gcggctaatg cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg
    26581 gggattccag caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca
    26641 tttccgactt gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac
    26701 gcaatgacaa atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat
    26761 gtagcgccac cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc
    26821 ttagttttac tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata
    26881 ccaaaagcac ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta
    26941 tctccagata ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct
    27001 gttccccata ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg
    27061 tactgatcgt aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga
    27121 tttttttctc cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca
    27181 cgttctgctg gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc
    27241 tcgtaagcat aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt
    27301 gcatctttgt attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt
    27361 atccatgcaa agatgactac accaaaaata atgacatacg tatggggtgt cgataattta
    27421 gtgaagtcgc gtttcggttt tcctgaagaa t
//
LOCUS       DAYYHS010000033        26915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000033 DAYYHS010000000
VERSION     DAYYHS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.033"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1086
                     /locus_tag="ACHA6A_001367"
     CDS             82..1086
                     /locus_tag="ACHA6A_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969573.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1087..2031
                     /locus_tag="ACHA6A_001368"
     CDS             1087..2031
                     /locus_tag="ACHA6A_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969574.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2031..2993
                     /gene="opp4B"
                     /locus_tag="ACHA6A_001369"
     CDS             2031..2993
                     /gene="opp4B"
                     /locus_tag="ACHA6A_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8969575.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3020..3934
                     /locus_tag="ACHA6A_001370"
     CDS             3020..3934
                     /locus_tag="ACHA6A_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969576.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3960..5741
                     /locus_tag="ACHA6A_001371"
     CDS             3960..5741
                     /locus_tag="ACHA6A_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8969577.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6089..6775
                     /gene="rnc"
                     /locus_tag="ACHA6A_001372"
     CDS             6089..6775
                     /gene="rnc"
                     /locus_tag="ACHA6A_001372"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8969578.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6795..10376
                     /gene="smc"
                     /locus_tag="ACHA6A_001373"
     CDS             6795..10376
                     /gene="smc"
                     /locus_tag="ACHA6A_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8969579.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10385..11194
                     /locus_tag="ACHA6A_001374"
     CDS             10385..11194
                     /locus_tag="ACHA6A_001374"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8969580.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11205..12203
                     /gene="ftsY"
                     /locus_tag="ACHA6A_001375"
     CDS             11205..12203
                     /gene="ftsY"
                     /locus_tag="ACHA6A_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8969581.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12390..14657
                     /gene="gshAB"
                     /locus_tag="ACHA6A_001376"
     CDS             12390..14657
                     /gene="gshAB"
                     /locus_tag="ACHA6A_001376"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8969582.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14746..15198)
                     /locus_tag="ACHA6A_001377"
     CDS             complement(14746..15198)
                     /locus_tag="ACHA6A_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8969583.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15348..16298
                     /locus_tag="ACHA6A_001378"
     CDS             15348..16298
                     /locus_tag="ACHA6A_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8969584.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16291..17250
                     /locus_tag="ACHA6A_001379"
     CDS             16291..17250
                     /locus_tag="ACHA6A_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8969585.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17247..18002
                     /locus_tag="ACHA6A_001380"
     CDS             17247..18002
                     /locus_tag="ACHA6A_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969586.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18025..18978
                     /locus_tag="ACHA6A_001381"
     CDS             18025..18978
                     /locus_tag="ACHA6A_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8969587.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19622..19918)
                     /locus_tag="ACHA6A_001382"
     CDS             complement(19622..19918)
                     /locus_tag="ACHA6A_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8969588.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20273..20629
                     /locus_tag="ACHA6A_001383"
     CDS             20273..20629
                     /locus_tag="ACHA6A_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8969589.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20639..21538
                     /locus_tag="ACHA6A_001384"
     CDS             20639..21538
                     /locus_tag="ACHA6A_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8969590.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22058..22786
                     /locus_tag="ACHA6A_001385"
     CDS             22058..22786
                     /locus_tag="ACHA6A_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8969591.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKSVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22931..24958
                     /locus_tag="ACHA6A_001386"
     CDS             22931..24958
                     /locus_tag="ACHA6A_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8969592.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25066..26163
                     /locus_tag="ACHA6A_001387"
     CDS             25066..26163
                     /locus_tag="ACHA6A_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8969593.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26470..>26915
                     /locus_tag="ACHA6A_001388"
     CDS             26470..>26915
                     /locus_tag="ACHA6A_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969594.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         9021 a         4660 c         5685 g         7549 t
ORIGIN      
        1 tgttataaaa cgctttattc tgtgtaatgt ccttttttat taagaaaaac ttccatttag
       61 gaagcagaga ggggtattcg attgtccgat aaccaattat tagaagttca gaacctacat
      121 acaggattcc gactcaaaga tgactattac gatgcagtag atgacgtttc atttacttta
      181 gacaaaaacg agattttagc aatcgttggg gaatctgggt gcgggaaaag taccttggct
      241 actacgatta tgggattact tgatcctaat aacacaaaaa ttacaggtga gattttatat
      301 aacgatctga atttaatcga cctgaacgaa acgctctata ataaaatccg cggaaatgat
      361 atcggaatga ttttccaaga tccgttgtct gcattaaatc ctctcatgag aatcgaagac
      421 cagatcaaag aaggactttc ctatcacaca aagatgaatg cagaacagcg tcaagctcgt
      481 gcactagaat tgctagaaca agtagggatt cctaatcctg cgagagttgg acgccaatat
      541 ccccatgaac tatcaggcgg tatgagacaa cgtgtcatca tcgcaatcgc aatcgcctgc
      601 aaacctccaa tcgtcattgc agatgaaccg acgacagccc tagatgtaac catccaagca
      661 cagattcttg atttactgaa agaccttcag gaagaaacaa aaacagggat catcttgatc
      721 actcatgact taggagtcgt agcagaaatg gctgatcgag tggcagtgat gtatggcgga
      781 cagttcgttg aagtagcagg tgtaaaagaa ctgtttgatc atcctaagca tccttataca
      841 cgctctttac tgaactctat accacaagaa ggaaatcatg atgcagaact ccatgtgatc
      901 gaaggtgtcg ttccttctct gaaaaacatg cctcgtaaag gttgtcggtt tgcaccacgt
      961 attccatgga taccagcaag cgatcatgaa gaagagccta tccttcatga agtcgcaccg
     1021 aaccacttgg tacgttgctc ctgctataag catttccact ttagagacga aaaaggagag
     1081 gtgtagatga cagaattaat cacaatcaaa gacctgaaag ttcattaccc tattcgcagt
     1141 ggattcttca atcgagtaac ggatcatgta tatgctgtag atggtgtgga ttttattatc
     1201 gaaaaaggaa agacatatgg tttagtggga gagtctggct ctggaaaatc tactacggga
     1261 aaagccgttg tgggattaga aaaggtaaca gccggacaaa tcatgtatga aggaaaagat
     1321 gtaacgaagc gaagcaaccg caaaaaaatg ggttataaca aggatgtgca gatgatcttc
     1381 caagattcaa tgtccagttt gaatcctaaa aagcgagtgt tggatatcat tgctgaaccc
     1441 atccgaaatt ttgaacggtt aagcgatcaa gaagaaaaga aaaaggtaaa aggactgcta
     1501 gatatcgttg ggatgccgga agatgcgcta tataaatatc ctcatgaatt ttcaggcgga
     1561 caacgtcaac gtctaggagt agcccgtgct gttgctacca atccaaaact gatcgtagcc
     1621 gatgaacctg tatctgctct ggatctatct gttcaagcgc aagtattgaa tttcatgaaa
     1681 cgaatccagc aagaatacgg cctcagctat ttattcattt cacatgattt aggtgtagta
     1741 aaacacatgt gcgacaatat cgcaattatg tacaaaggtc gatttgtaga aatcggtaca
     1801 agagaagaca tttataatga cccgcgacat atctatacga aacgtctgct ttcagctatt
     1861 ccgcaaatcg atgtagaaaa tagggaaagc cacaaagaaa atcgacgaag agtagaacaa
     1921 gagtatatcc aaaaccaaaa agaatactat gatgcgactg gtcgagtcta cgatttgcgt
     1981 acgatcacaa ctacacacaa agtggcgttg aaagatggag gtgcaagcta atgtggaaaa
     2041 cgattctgcg acgtgtttta ttgatgatcc cgcaagttat tattttaagt gttctgatct
     2101 tcttattagc ccaagcgatg ccgggggatc catttaccgg tttgatcaat ccaaatcagg
     2161 atccggcagt gatcgaacag atgcgacaag cagctggctt gaatgaccca tggtatgtcc
     2221 agtacttccg ttggatcgga aatgctttgc aaggcgattt tggtcaaagt ttcttataca
     2281 aacgctctgt tgctgacttg atcggcgaac ggatcatcaa cactttatat ctatcgatct
     2341 tgacagttgt ccttacttat ttgattgctg tgcctctggg gatgcttgct ggccggtatc
     2401 aaaattcaat cttggataag attgtcgtaa tctacaactt ctttagtttt gctgttcctt
     2461 tatttatctt cggcttgatc atgctatttg tttttggtta tcgtttaggc tggttcccaa
     2521 caagtggatc acaaacacta ggtttgcaag gtggatttat cactcaatgg atagaccgtt
     2581 tgaaatatat tctgttacca tcaattaccc aagcattttt ggcgactgcc gtcacaatcc
     2641 aatatctgcg aagtgaagta atcgattcga aatcgctcga ttttgtcaga acagcccgtt
     2701 ccaaaggggt accgacgaat aaagtattca gtcgacatat tttccgaaat gcagcgttgc
     2761 cgatggcttc tcaaatgggg tatgaaatca cttcgttgat tgctggttct gtagttatcg
     2821 aaaagatttt tggttatccg ggagtcggaa aactatttat tgattccatc ggccaacggg
     2881 actataccgt gatcacatcc ttagtattga ttttaggaat cgcgacgcta gtggggacat
     2941 tactttcaga tatcatcatg agtatcgttg acccgcgtat tcggattcaa taaaaccaat
     3001 cagaaaggag aatggatcga tgagtgaaaa aaacaataca gcagttcaag aaagtattcc
     3061 tcctatgggg atacgaatga ttgctcgaga atttaaaaaa gataaaattg ctattttttc
     3121 attaggtttg ctagtggttt tattattagt gatctttatc ggtgcgatgc tgaccgatca
     3181 agacaaagtg atgtatgtta gtatcttcga caaatacgct gcaccaggaa cagtttcgac
     3241 acaaggacaa aagttcattc tgggagcaga tgaaggcgga cgtgatgtat taggacaatt
     3301 gatcataggt gctagaaact ctgttacgat cggatttgcg atcacgatcc ttacttcgat
     3361 catcggggtt ggattaggga tcatctctgg ctactacgga gggatcattg acaacatctt
     3421 gatgcggatc gttgatttta tcatgattct accgatcatg ctgatcatta tcgtcttcgt
     3481 atcgattatt tccagataca atgtctggtc ctttatattc atcatgagtg cattttattg
     3541 ggtagctaaa gcccgattat tccgaagcaa gacattatct gaagcaagaa gagattatgt
     3601 cagtgcctca aaaacaatgg gaaccaatga ttttaaaatc atgttccgcg aaatcatgcc
     3661 gaatctaagt tcattaatca ttactaattt gacgatgaac tttgcggcaa acatcggtat
     3721 cgagacaact ttgactttct taggtttcgg cttgccggca aatgtaccta gtttaggtac
     3781 attgatcggt tatgcaagta acggagaagt cttagtgaat cggcaatgga tctggttacc
     3841 cgcatcaatc cttattttag ttttgatgtt gagtatcaat tatgttggac aagcattcaa
     3901 gcgttctgca gatgcgcgcc aacggttagg ctagtataga agcgaagggg gaaataggaa
     3961 tgaagaaaac gatttttgga tttgcagcgc tgatgtctgc tgtcgttctt gcagcatgcg
     4021 ggggcggagg tacagatacc tctggaaaca actctggagg caacggcaat aacaaagagg
     4081 aattaacttt tccaattgca acaaaaaata atgatgatgc aatcgaagac ggcgaattag
     4141 aagctgcggt cgcaacggac agtcagttta aaggattgtt ccagtgggag ttttaccaag
     4201 attcatatga tgcagctttt atggctcctt ctcatgaacc gctatttgca aacgatgacg
     4261 atttcagaat cacagacgac ggagctgcaa cgcttgatct agatgaagat acgaaaaaag
     4321 cgacaatcaa agtaaaagac aacgtcaaat ggtcagatgg tgaagatgta acggcggacg
     4381 atgtgatttt cccttacgaa atcattggga ataaagatta cactggtatc cgttatgacg
     4441 acactttccg taacatcgta ggtatggaag aatatcatga tggaagtgcc gacacaatct
     4501 ccggaatcaa aaaaatagac gacaaaacag ttgaaatcga atataaagaa gtaaacccaa
     4561 gcatgcttca atcgggtggt ggtatctggt catatgcagc accaaaacat acgttagaag
     4621 gtgtagcaat caaagatatg gaatcaagcg atcaagtacg taaaaatcca gtgacttttg
     4681 gcccttatta catgagtaat atcgttgcag gagaatcagt agaatacttg ccaaacgagc
     4741 actactggaa tggtacgcct aaattgaaaa agatcacgat gaaatctgta ccgatggcaa
     4801 gtgcaactga agcattgaac tctaaattat atgatatgat tttccaaatg ccaacagata
     4861 cttacgatac ctataaagaa attgctggct atacaaactt aggccgtgaa caaacttctt
     4921 atacttatct aggattcaag ttaggtaaat ggaatgcaga tacaaagagt gtggaataca
     4981 atccagattc aaaaatggct gataaatcat tacgtcaagc aatgggctat gcattagata
     5041 ataatgcagt aggtgaacgt ttctatgcag gtttgagaag caatgcgact tcattgattc
     5101 ctccagtatt ccagtcatac catgatgatg agatgaaagg attcacacaa gatctagaca
     5161 aagcgaaaaa actattagat gatgctggct acaaagatgt ggatgacgat ggtttaagag
     5221 aagacaaaga tggaaataaa ctaacaatca actttgcttc gatgtccggt ggggaaacag
     5281 cgcaaccatt ggcagattac tatatccaac aatggaaaga aattggtttg gatgtccaat
     5341 acacaacagg tcgtctgatc gaattcaatt ctttctatga taaattagaa aacgatgatc
     5401 ctgaaattga tgtttaccaa gctgcttgga atacaggaac tgatccaaat ccaagtggac
     5461 tatggggacc aaatgctgcg ttcaactaca ctcgttatga atcagaagaa aatacaaaat
     5521 tgatcaacga tatcagttca agcaaagcat ttgatgaaga gtaccaaaaa gaagcatatc
     5581 aaaaatggca acaatatgca tttgaagaag catttgcgat tccaacgttg ttccgtaatg
     5641 aagtattgcc agtcaacgac cgtgtcaaag actgggattg gtcttatgat gtaccaaacc
     5701 catgggcaaa tgtttctgtt acagcagatt cacgttcata atcactaaaa atgaccgaaa
     5761 aagggcgatg tcctttttcg gttttttgat atttttctgt ttatttctct aatattcagg
     5821 tcaaggcgct gatttcatga cttctccaat aaacggtgtt caaagtccag tttctcggta
     5881 ttaagtcaaa tttatgcaaa aatatggaaa gccattttca caaaattttt tcttcatact
     5941 cgaaactaaa cgaatttttc acaacttctc gtcaatcact aggattatta aagctttact
     6001 taccttttga ccgaaccatt tcaaatttga caaaaaacca gtataataag gaagtatcga
     6061 aaaaatatcc ataaaaggaa tgtggaaaat ggacaaccag ctgacaaaag aactaaaaga
     6121 aaactataac atcgtttttc acgatctaaa tttgcttgaa caagctttta cccattcatc
     6181 ctatgtgaat gagcatcgca atctgcaatt atcggataat gagcgacttg aatttttagg
     6241 agatgccgtt ttagaactga tggtttctca gtatttattc cgtttatatc ctcatttgcc
     6301 agaaggaaaa ttgacaaaaa cacgagctgc gatcgttcgc gaagacagct tgtcgaaatt
     6361 tgccaaagaa tgtcactttg atcaatatgt tttgttgggc aagggagaag agaattcagg
     6421 aggacgtacg cgaccagcat tgctttgcga cctattcgaa gcttttttag gcgcgttata
     6481 tcttgaccag ggattcgaca cgacccatgc attcatcgaa aaagtgattt ttcctaaagt
     6541 caaagcaggt gctttttcac atgagatgga tcataaaaca aaactacaag aagtcttgca
     6601 aaaatcaggt gatgtatcaa tcgaataccg attgatcaat gaagaaggtc cagctcatga
     6661 acgagtattt tggatcgaag tttatgtaga tgatcaattg attggcactg gacaagggaa
     6721 atcgaaaaaa ttagcagaac aagctgcggc ggaaaatgca ttggcagcac tgtaaaagta
     6781 agtgagggaa agctttgtat ttgaaacgaa ttgaaatagc cggttttaaa tcatttgcag
     6841 acaagacagt cattgatttt gaaaatagcg tcacagcagt ggttggacca aatggcagcg
     6901 gaaaaagcaa tattacagag gctatccgct gggtattagg agaacaatct gctaaaagtc
     6961 tgcgaggcgg aaaaatgccg gatatcatct ttgccggttc cgatacccgt aagcagctga
     7021 atattgcaga agtcacagtg attttagaca atagtgatca ttatctgcca ttggagtaca
     7081 atgagatcag tgtaacccgc cgttatcgac ggactgggga aagcgagttt ttcatcaata
     7141 aacaaagctg cagattaaaa gatatccaag aattgttctt agattctgga ttaggaaaag
     7201 aatcgttctc aatcatttct caaggaaaag tagaagcgat tttcagtagt aaaccggaag
     7261 accgtcgtgg gatctttgaa gaagcagctg gtgtcttgaa atacaagcag cggaaaaaga
     7321 aagcagaaca gaagcttttt gaaacagaag acaacctcag ccgcgtccaa gacatcatcc
     7381 acgaattgga agaacagtta actcctttgg ccgcccagag tgaggcagca aaggagtttt
     7441 tacggctaaa agaaacactg actcaaaccg atgtctcttt gatggttgct gaaatcaaga
     7501 cagcaaaaaa agattgggac aacaaacaag cacagcttgc gaaattcaat ctagaattag
     7561 gcaaactatc tgagtcgatc caagaacaag aatccatttt ggcgaagcag cgtaaagaaa
     7621 atgcgcaagc cgatcgtctg atcgaaaaaa atcagcaagt attattagat ctttcggaaa
     7681 aactgaagca gacagaagga caaaaagatg ttttgcagga gcggacaaag catacacaaa
     7741 aaagctcgca agaatatcaa acttcattag cagaagcaca aaaaaaggtt aaacattttg
     7801 aaaagcttca agaaagcttg atgaaagcag cagcagaaaa agaaacagag atccaaaaag
     7861 ctgaagcaaa tctcataaaa acacagcaag aattagaaaa ataccaaaaa tcaacgaaag
     7921 aattattggc agaattaaga gatcaatatg ttgatttgat gcaagaacaa gcagctgtag
     7981 gaaacgaact gaagtatcta gaacgtcaat atatacaaga aacagcaaaa agcaagcaga
     8041 cgttagccaa gcaatcggaa gtagaagcat cagtagaccg attgatctta caaaaagaag
     8101 aattgacaca aaagcaggcg cagctaaaat catctttaac tgaaacaaaa gagaagttag
     8161 aaatgatcca acaaaacggg aaaaagttcc aagaagcatt agcaaaagaa cagcctaaaa
     8221 tgtatcaatt aatgaatcaa gtccagcagc tacgtgctcg taagaaaagc cttcaagaaa
     8281 tccaagaaaa ctattttggt ttttaccaag gggttcgatt ggtcttgcag cataaacagc
     8341 agctttcggg tattgtcgga gcagtggctg aactgatcga tgttcctgca gatttcacgt
     8401 tagctatcga agcagcttta ggtggggcag cccagcatgt catcgttgaa aatgaaaaag
     8461 atgctcggca agccatcacg tatttgaaac agcaaagagg aggaagagca acattccttc
     8521 cactgacgac aatcaaacca agacaattac cggctcatat attgacacaa gcagcagctg
     8581 tagaagggtt tatagggatt gccagtgaac aagtcagtta tcccgatcaa atccaaactg
     8641 tggttcaaaa cctgctagga acgatccttt tagccaaaga cttaactagt gccaatgcaa
     8701 tcgcgcaaac gatccgctat caatatcgag tggtttcctt agaaggtgat gtgatgaatg
     8761 ccggaggatc catgacaggt ggagcgaata aacgaggaaa ccaaggcagt ttgtttgtac
     8821 aaaatcaaga attgaaacag ttgactagtg aatttgaaga agcagataaa caactccaag
     8881 cacaagaaaa aaaagttcag gagctgcaac aagaaacagc tcgtttagcc gaagaacaag
     8941 aagttttgcg tacacgaggc gaacaattac gttttgaaga acaagaagcg acaaatcagc
     9001 tacaaaacat cataaatgaa cttgaacgtt ttgaaaaaga aaagcaaatc tcaacttttg
     9061 agacacgtga actgcagcag tttatcgaag attacgaaaa gcaaacgaac gaattgaaag
     9121 acaaacaaac agatcttgaa tcgcaacgac aaaaaatcga cgaagaaatc aaatcgttaa
     9181 gtcaagaaag cgaccagatg gaagccagaa gagcacaagt acaaagtcaa aaagcacaag
     9241 aacaagcaga tcttgctgtc ttgaaagagc agtttaatca tctgcagatc cagctccgcg
     9301 gtgcccgagt ccaaaaagca gaggcaaccg aacgtcaaga agcgatagaa aagcagttgg
     9361 cgacattgac agcagacttt tctgatcatg aagtcacaga agaaagtttg gaaaaacaaa
     9421 ttaacgaatt atctgctcag cgagaaacat taaaagccga gttggcaaaa gccaaagagc
     9481 agcgagatag aaggcaaaag gagatcgata agcttgaagc agtcttggca gagcgtaatc
     9541 gtgagcaaaa agcacgactg tcggaacaat ccaaattaga agtccaaaaa gaccgagctg
     9601 aaatgatttt agataatcat ttgacctatc tgcagtcaga atatcagctg acatttgaga
     9661 aagcttccca agactatcaa gaaacaaccg atatcgaaga cagtagaaca aaggtttcat
     9721 ccttaaaaga acagattgaa aaactgggac cagtaaactt aaattcaatc gaacaatacg
     9781 aacaagtcag tgaaagacat acattcttag caacacagcg agatgatctg ttagcagcga
     9841 aaaaccaatt gtttgaaacg atggatgaga tggatgatga agtgcggaca agattcaaag
     9901 aagtatttga agctatccgt caagaattca aaattgtctt tccaaatatg tttggcggcg
     9961 gtaaagctga attagtactg acggaaccaa ctgatttgct gaaaacaggt attgagattg
    10021 aagtacaacc tcctggcaaa aaactgcaaa gtctaagttt gctttctgga ggagaacgag
    10081 cacttacagc catcgcgttg ctcttttcca ttatccgtgt ctgtcctgtt ccgttttgta
    10141 tcttagatga ggtagaagca gcgttagatg aagccaacgt gaaacgtttt ggtcgttact
    10201 tgagcgattt ccaagatgac acacaattta tcgtagtcac acaccgcaaa ggaacgatgg
    10261 tcgcagcaga tgtactgtac ggagtgacga tgcaggaatc cggtgtatca aaaatcgttt
    10321 ctgtacggat ggaagatatc aatgaagagg cgaaaatcaa cgaaacgaaa gtgtaggaac
    10381 gaaaatgatc aaattagtag caatcgattt ggacggtaca ttattaaatg aacaaaagct
    10441 gatttcagat gagaataaac aagcattagc acaagcaaaa cagcaagggg tcaagatcgt
    10501 tctatgtaca ggacgcccct tagctgcgat ggcgcattat ctgcaagaat taggactggt
    10561 agatgaaggt gactacagca tcactttcaa cggcggtttg gtacaaaaaa atgatactgg
    10621 tgaaatcatg gaaaagaaag tgatggaagt atcagatatc caacgcttat acaaactggc
    10681 tcagcaattg gatctgccac tagatgtttt gtctgacaat gtggttttac agctgcctag
    10741 tgccccaagg aagcaatcct tgtacaatgt gttgaataag ctgctgcaat tccaaccagc
    10801 ggatataagc gatattacag atgaatttgt gcttaataag gcagtaatcg cttatgacca
    10861 agaagaatta gatcctaaaa tcaaagaaat accagcagaa ttccatgaaa tgtatgagat
    10921 catcaaaaca agaagtatgc ttttggaatt tatgccaaaa ggtgttacaa aagcttatgg
    10981 tatttcttta ttggcaaaag atcttggttt gaaacaagaa gaaatcatgg cgatcggtga
    11041 tgaggaaaat gatttaccga tgatccaata tgctggacta ggcgtagcga tggagaatgc
    11101 tgtgcctttc gtcaaagaag cagcagattt cgtgacagcc agcaatgaag aaaatggcgt
    11161 agccaaagtg atccaaaaat ttgtattaga gtagggggag tcaaatggga ttttttgata
    11221 aaatcaagcg tgcttttact ggcgaagaga aaacagaaga agttgaagta caggagaagt
    11281 acgacaaggg tttagaaaag acgcgtaaaa catttggtga acggatgaat gagttgtttg
    11341 ccaattttcg aacagtcgat gaagactttt ttgaagaatt agaagaaaca cttatcggtg
    11401 cagatgtagg ttttgaaaca gcgatacgaa tcaccgaagc attacgtcaa gaagtgaaac
    11461 tcagaaatgc gaaaaaatcg gcagaagtcc aaaatgcgat catcgagaaa cttgtggatc
    11521 tctatgaagc ggaaggaatc aatgaagtaa atgaactaaa tatccaagaa gacggactaa
    11581 cagttatttt gtttgtcggt gttaatggtg tagggaaaac aacaagtatt ggtaaacttg
    11641 cccatcagtt caaaaacgaa ggtaaaaaag tattgatggc agcagcagat accttccgcg
    11701 ctggagcaat cgaccagtta gttgtctggg gcgaacgcgc tggtgttgaa gttgttcgtg
    11761 gcaatgcagg aggcgatcca gcagctgtcg tattcgatgc tttggaaaga gcaaagacag
    11821 agcaggcaga tgttttacta gttgatactg caggccgact gcaaaataaa gtgaacttga
    11881 tgaacgaatt agaaaaaata aaacgagtaa tccagcggga attaccagaa gcaccccaag
    11941 aaacacttct tgtattagat gcaaccactg gtcaaaacgc gatggttcaa gcaaaacaat
    12001 tcaaagaaac aacagatgtg accggtcttg ttttgacaaa attagatggt acagcaaagg
    12061 gcggtatcgt acttgctatc cgtaatgaat tgcatttacc agtgaaattg gtcggtcttg
    12121 gtgaaggaat cgatgacttg gagccgtttg atccaaatga ttttgtcgtt ggattgttca
    12181 aaggattact aaaagaagaa taacccatgt ctaaaaagca ggagctgtga caaaagttgc
    12241 agctccttgt ttgaatgatt gtttttaaca tagctgtgtt tttgatgcct tcatttttat
    12301 acttatttgt aagcggttct caaacaattt cgttcttttt cttagacttt ttgattataa
    12361 ttagattcat atgaattgga ggtcacttaa tgatgaattt taagcaatta ttattgcatg
    12421 tcaatgcgcg cccttttatc gaccaagctc gatttggtat agaacgggaa ggacagcggg
    12481 ttgatcttgc aggaaatcta gcaaaaaccg atcatccagc aatctttggc gatcgatcct
    12541 atcaccccta tatccaaaca gattttagcg aaacacaaac agagatgatc acccctgtta
    12601 ccgattctat tcccgaatta tttcagtatc tcgctgctgt ttatgatgtg actgctcgtt
    12661 ctataccgaa agaagagatg atctggccat taagtatgcc acctgcctta ccggaaaaag
    12721 acgaagagat cattattgca aaattaaaaa atttcgaaga tgtcttgtat cgacgttatt
    12781 tagcaaaaaa atacgggaag cgaaaacaga tggtaagcgg tattcatttc aattttgaat
    12841 ttggcgatga gttgctaaga acattgttca gccatcagga agaatttcaa gatttttccg
    12901 aattcaaaac agaactttat ttgaaaacag cgagaaactt tatgcgctat cgctggatga
    12961 tcacttattt attcggtgct tcaccaatga gtgagaagaa ctacttttta gatgaatcac
    13021 atccgcaaga acctgttcgc agtattcgca acagtgcgct gggctatacg aatcatccaa
    13081 atgtgaaagt ttcctatgct tctatgaaac agtatttagc agatatcgag cgaatgatcg
    13141 aagaagggaa actttcggag gagaaagaat tttatacacc gcttcgtttc cgaggcggaa
    13201 agaaagtcgc agatttagca acaacaggtg ttcgttatat tgaattgcgg aatatcgatt
    13261 tgaatcctta tgcaagattg ggtatcaatc cagagcaagt tcggttctta caattattcc
    13321 tgatgtacat gttatggaca gaagaaaaag aagactgtga ccaatgggta gcagaaggta
    13381 cgactcgaaa taacaaagta gcactagaac aaccatcaga tcaaacagaa tttcatcaag
    13441 aaggtagaga aattcttgaa ggaatgaagc aaatgctggt tgaattggat tggttggatt
    13501 ctctttattt agtagaggaa gcattgactc aaatggatca tcctgaacaa acgttagcag
    13561 cgaaactcta tcaagaagca cagctatcga gtcagcaaga agttgctgtg gcattgggac
    13621 atcaatatta taaagaaagc catgaacgtc cctatcagtt ggccggtttt cgcgaaatgg
    13681 aactttctac tcagatattt atgttcgatg ccatccaaaa aggtgtccaa gtcaaagtat
    13741 tagatgagtc ggatcaattt ttgcgtctgc aattccaaga ccatgtagaa tatgtgaaaa
    13801 atgcaaacat gacaagtaag gacagctata tcgtcccact tatcatggaa aacaaaacag
    13861 taacgaaaaa agtcttaaaa gaagctgggt tccgggtacc aggaggcgca gaattttcat
    13921 ccatggaaga agcggtaaaa gcttatccga gatttgccga gcaggcattt gtgatcaaac
    13981 ctaaatcaac caattacggg ttaggtatca cgattttcaa ggaaggagct agtctggaag
    14041 attatcaagc aggtttagcc attgcttttc gtgaagacag ttcggtttta gtagaagaat
    14101 ttatgccggg aacggaatat cgtttctttg tgatcgacgg agaagtccaa gccatcatgt
    14161 tgcgagtccc tgctaatgtt attggtgaca gtatccgaac agtgaaagaa cttgttgaag
    14221 aaaaaaatag tgaccctttg cgtggaacga atcatcgtgc acctttagaa ttgatccaat
    14281 taggtgaatt agaacaattg atgttgaaag aacaagggtt gacgatcgaa tcagttcccc
    14341 aagccaatca aattgtttat ctaagggaga actcaaatat cagtacaggt ggtgattcga
    14401 tcgatatgac tgatgaattt tctgaagctt acaaaaaaat cgccgtttct gcggtagaag
    14461 cactaggagc taagattagt ggtatcgatt tgattatacc agataaagaa atcgatccta
    14521 caactgataa aaaagcatat gggatcatcg aagcaaattt caatccagcc atgcatatgc
    14581 atgtttatcc ttttgcagga aaaggcagaa gattgacgat gaatgtattg aaattgttat
    14641 atcctgaagt attttaaaag aaaaagcttt tgacagattg tcctatttga tgtcaatctg
    14701 atcaaaagct ttttttgcat gtaaaaattt atgtgttttt tcgttttaaa ataaacgatc
    14761 ccagaagctt tttttctttt ttggtttttc tttttcagct ggcgaagagt tttcttctgg
    14821 gtattttttt tcgatatcaa tcacaggttc atttactgct tcttttgctg ttaagagcaa
    14881 tccgaattcc tcaggatttg cagtgacgta atcgttgact acagtgaaac ggctattgtt
    14941 tttcgttgct agctggatat attggttttg gacagaaagg ggtagagccc cattcaagag
    15001 tatggaagca tctggatgtt ttttgagttc ttttaaaaag ttttctttgt tttttggttg
    15061 gatcatttca gcaacagtca tactaagata gcagcgttcc ctgaatgtcc ctaaatattt
    15121 atgctgttct tcagggttca ctaaaggtgt accataaagt cccttatcca aatggctttt
    15181 tacatcatct tgtgccataa aaaataccct ccttcttctc tttgtctttc ttacgattat
    15241 agcatattcc caataatacc taaaacatat gaaaaagcgg ttgcttttgt tcaacgattc
    15301 acttaaaata agaatgaaaa tcattctcaa ttagaaaggt ggattggatg cgctacggac
    15361 gactagtagc attattagtt attttaagta gtatttcatt gttcatcgga gtatcgaatg
    15421 tacaattgtc agaactgctc cagttagatg ggcaaagctt acaaatcttg ctagtgactc
    15481 gtttgccacg aacgatttgt ttgatccttg tgggagcgac cagtagtatt tgtgggttga
    15541 tcatgcaaca tttgacccaa aataaatttg tttcaccaac gactgcgggc acgatggata
    15601 gtgcgcgttt aggtatttta gtggctatga ttttcctacc tggtgcatcc ttgattgtcc
    15661 gatccttgac agcattttgc tttgcttttg caggaacatt gcttttttta tcccttacgc
    15721 gattgttcct ccagaaaaat caattgatgc ttccgttgat tggagtcatg ttagggaata
    15781 tcattggttc agttgctact ttttttgctt atcaattcca gttggtacaa aatatgactt
    15841 catggcttca agggaatttt gcgactgtga tacgaggaaa ttatgagtta ttgtatgctg
    15901 ttgttccatt gttgctcttg gtttggctat ttgcttaccg atttacgatc attggtctgg
    15961 gcgaatcgtt tgcagtaaat ctgggaatag attaccagaa gatgcagttt ttaggcattg
    16021 gcttagtatc attggctagt gcagtcatgt tgatcatggt aggaagcatt ccttttatcg
    16081 gagtgatcat tccgaatctt gtctctagaa tatatggaga tcaagtgcac aaaacaattg
    16141 gcgtaactgc tttagcaggt agtttgttct tagtattttg cgatcttgtc gctcgcgtgt
    16201 tgatttttcc ttatgaaata ccagtcagtg tgatcgttgg aatcatcggt ggggctatct
    16261 tcctttatct tctgataagg ggacgacgat atgcttaaaa aaaagccatc tatttttatt
    16321 ggtatgcttg ttcttgtttt atttgttaca ggaatgtact tgacgatcca tacatatgga
    16381 aactggcatt ttgcatgtgc attgaggagt aagaaaattt ttgcatttat ccttgtctcg
    16441 ctagctgtca gcagtgccac aatcagcttt cagacaatta cgcataacca gtttttgact
    16501 ccttcagtat tgggacttga gtcgttatat gtccttgtcc aaacggtcct tttcttcttt
    16561 atcggaggag tgacgatgct tcagcagaca ggtacagttt ttttcttatt gaacgttggg
    16621 ctgatggtcg ggctgagtct attgttattt ttctttcttt tgaaaaaaat gagagcgaat
    16681 ctttttttat tactgatgtc agggatgatt ttaggtacgc tttttggtag tgtcagtacc
    16741 tttttacaag tcatcatgga tccgaatgaa tatgatttgc ttcaaggcag attatttgca
    16801 agctttggca atatccagag tcagtatctt ttgcctgctt ctttgatcat tatcggctgc
    16861 tggtacctgt tatttcgata tcgacatgtg ctagacgttt tccatctcgg aaatgaacaa
    16921 gcagtaaacc tcgggataga cacagatcgt tttgaatttt tgattttatg ttgtgtcagt
    16981 cttttgacag ggacatcaac ggctcttgtg ggtccagtta cttttttagg gtttattgtc
    17041 gcaaatatca gttatcgtgt cttttctact tatcgtcatg gtcagctatt tatcggaagc
    17101 tttctggtag ctatgctgtt tctgacagga ggacagctcg cagtagagca gattttcggc
    17161 tggaatacaa cagtgagtgt cgtcgttgaa tttgtcggag gaatttattt tgtcacaaaa
    17221 ttgattcgcg aaaggaagag tatcctatga ttgatttaca aaaggtcaat aaactgtacc
    17281 atggaaaatc agtggttaaa gatgtacagt taacgattcc tgaaaagaag ttgactgctt
    17341 ttatcggtcc aaatggtgct ggaaagtcaa cggtgttgtc gatgatcagt cgtttgatac
    17401 caaaagatac aggtgaaatc tatttagacc ataacgaagt aaaggcttgg aaatccaatg
    17461 aattagccaa aaaattatcg atcctacgac aatcgaatga aataaatgtc caaatcaccg
    17521 tgaaagaatt agtagaattt ggacgatttc cttatacgaa aggaaaattg acgacaaaag
    17581 acgaggaaat cgttcaggaa tcactggaac accttgggtt agaagaaata gcggatcaat
    17641 cgattgacac tctttcaggc ggtcaactcc aacgtgcgta tattgcaatg gtgctagcgc
    17701 aagacacgga ttatatttta ctggatgaac ctttgaacaa tctagatatg aattttgcgg
    17761 ttcaaatcat gcagatttta aaaaatttag tacaagaaat cgggaaaaca attgttattg
    17821 ttcttcatga tatcaacttt gctgcaagtt atgcagacga aattgttgca atgaaagatg
    17881 gacagttatt tacgcaagga acgacccaag aaatgataaa aaaagatgta ttggatgaat
    17941 tatacgaaat ggacatacgg atttgtgaga ttgaaggccg cagattctgt ctttatttct
    18001 aaaactaaca ttaggagaga agagatgaaa aaattagtag taggtatctt gattctagca
    18061 tcatttggtt tagcagcatg tggaaatagc aacaacacca gtcaggcaga cacaaaaaaa
    18121 agcagcacac aaactgaaac tacattgacg ataacagata gtaatggaga ccaaatcgag
    18181 gtgccaaata atccagaaaa agtcgtcgta ttcgacaatg gttctttaga tacgatggat
    18241 gctcttggtg ttggagataa ggtagttgga gcagctacaa gcagccttcc tgagtatctt
    18301 tcatcctata aaaaagtaga atcagcaggt ggaataaaag aaccagactt agaaaaaatt
    18361 aatcagcttc agccagatct aatcatcatt tctggccgac aaagagattt tcaaaaagat
    18421 ctaagtcaaa ttgctccgac cattttcctt tctttagatg cgaaaaatcc atgggaatct
    18481 ttccagcaaa atgtcactgc tttaggggag atctttggta agcaagaaga agctaaaact
    18541 caattagagg aattatctag tgcaatcgat cagacgaaaa agaaagcaga agctactgac
    18601 aaaaaagcac tagtcacact tgtgaatgaa gggcaattat cagcctacgg atctggttct
    18661 agattcggtt tcatccatga tttatttggc tttgaacaag cagatgatca aatcgaagca
    18721 tccactcacg gtcaaagtgt ttcttatgaa tatgtgctgg aaaaaaatcc ggatatctta
    18781 tttgtagtag acagaacaaa agctattggt ggagacgaca gcaaagatga tatttctgcc
    18841 aatgaactgg tagcacaaac gaatgctggt aaaaatcagc agatcatctc ccttgaacca
    18901 gatgtctggt atttaagcgg gggcggttta gaatcaatga aactgatgat cgaagatgtg
    18961 aatcaagcat ttaaatagca taaataacag aggattaaac tctcatacta tgtaacaata
    19021 atgtttctta cattcttaaa tagcaagagc ctatcctcaa agaagttatc aaaaaccatc
    19081 cgtaaaatcg tattgatgat tttacggatg gtttttattt atttgagaaa tcttttttca
    19141 acgtgaattc attatctaca tcatacgggt tgattctaga atggggagtg gcaaaaaata
    19201 gtgtgatcca ttttttcagc atcaagatcg cctcctttga tttgaatcaa aggtacaaag
    19261 cgaaacttag ataaaactta aatttagata tttttatttt gtaccgatct cttctttaaa
    19321 cgatgttcca tctgctgctt ctcggcaata agtccactag actagaaaat atgagaagct
    19381 attttctgtc cagcgttctt attactcaaa acaaatcgca gttgtcacaa tctcttctaa
    19441 acgatgttcc atctgctgct tctcggcaat aagcccacta gactagaaaa tatgagaagc
    19501 tattttctgt ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta
    19561 aacaatgttc aatcagctga ccttcggtat tagctcaaag cactgatttc acaacctcta
    19621 tttaggcatg tgtctcagca agcggttgat agttgatgat ctgcattttt cgataaaaat
    19681 cggcgggaag cttactccac cgttttagta attgatagaa ataactctct tctgctttga
    19741 ttttatactc gcttaatatc aatccaaaac tattcgtttt ttttgaagct aattttttat
    19801 aaatatccaa tgctttggtt ttatcttcat tgacaaagag tgtttttact tcaatataag
    19861 aaccctcgat attttgtgtt tcttcgataa aatagatgtt ttttatatat tccttcatag
    19921 tgattcctcc aaagaagtct tttatttgat aagtttactc taccgaagag ttgttaataa
    19981 tccgtgaaca aaaagtaatt ctttttccaa tagttgttaa aggtttttta ttttttatag
    20041 tattttttgt tatttaagaa aggaataagg ttaaacaaat aacaggttgc tttttgggtc
    20101 tttttccgtt caagaaactg aacgaagaaa agggaaagtt gtctcttttg tgataagaga
    20161 aatcataata aacgaatgac ttactttcca ataaattgag aacagtcaga accagacaaa
    20221 ttagttgcat ctgatgaata aagtgattat tctatgacat gaggtgatac agatgaaatt
    20281 agaagtgaca aaagaagcac aagaagtatt gaagaataaa ttagaagcag acgatcaact
    20341 tttattagat atcgaaaacg gtgacggtcc atttgcagaa agccaagtca gttgccaatt
    20401 ggatacggca tttcgattga tcattgtcaa aaaagataca caaacagatc taagtcttta
    20461 ttctgtagtg gctgatacac cagttggacc aatcaaaatc aaagacagtg caatacttta
    20521 catggatgat ccatccacac ttgctttaga accaacttat aatagtttac aattaaaagg
    20581 accaagcggc ttgagaaaag gcaacttgca agttgttcga aaagactaga gggataaaat
    20641 gggaaaattg gcgatcatca gcgacctaca tgtggacatc aataaattcg gcgaatcaga
    20701 gctgatgcag ctttggcaag ttttgcaaaa gcacaagatc actcggcttc atctagccgg
    20761 agatacggct aataaagtag accgttgcct agctgttgtc gaatttttcg tcaataaagg
    20821 attatctacg acatttcatt tcggcaatca cgagttggca gatgtgccca atgaacagat
    20881 gatggaccat tttccagacc cacatttttt aaatgaacgg tatattgctt taaatgagca
    20941 gaccgttctt ttagggatga atggctggta cgactatcag ttttccgaat tgaaagatta
    21001 cgaggcaatc gtacgaatga agcgtttgta ttggtatgat cggttcatcc agcgtgggaa
    21061 aacagatctc gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc tagaccagct
    21121 ggaagcaaaa aaacttgagg tgattttatc cactcatttc gttcctaaac gggcatttat
    21181 catgtatcaa tctgctcctt atgagcgttg gaacaaatta aatgcatttt taggatcaga
    21241 atcattcgga gaattacttg accgttattc aaatatcaaa caagtagttt ttggccatac
    21301 gcatcgtcgc tttgaagata aatccatcgg tcacacgatt tacagctgca gaccttttgg
    21361 ttattattat gaatggcagc tgacccgaga ttttgtcttg aaagaacagt tgctagacag
    21421 ctatgatcct atgaaattac gtgtcctctt gcgtgcccat cacccagctt ttcaggccta
    21481 tcaaacacaa catttgcaag aggaatttga acaagcgatg acaatcgttc cgtattgaga
    21541 cagcattagt aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag taagagcagt
    21601 agatccattt atcgtggata tgctgctttt tttgcggaaa ataatcagcg aatgtttatt
    21661 tgatcggatg atttgtaact cgcagagtac tagatgcaag cgtgtattct ttttttgaac
    21721 gataatcaca aaaaaaaata taatatacat tttttattta attataatta atattgattg
    21781 aagtctaggc agtcaagtca cattttgata gataggaggg ggacttacga attattttaa
    21841 aggctatgac agctgtattt ctagattgtg tttgcatcaa gttgcaataa aaactagaaa
    21901 tcaaatcggg ctgataatca tttataacta atcgatacat aggaccaaat ataggtctat
    21961 gttcttttat ttcataagta tcgtaaaaca gaattttgct atggattaat ccaatcgact
    22021 gatatgttta gtaaataaag aaagaggaat gtcaatgatg aaaaaagaaa tcgtattaac
    22081 agcagcaatg atgttattca gcatggtagc aagtacgact tttgtttctg caactgaagt
    22141 atacccaaag gaatataaca cagaaggaac gattacattt gaagcaggag atgagggtgt
    22201 aactcctcct gttgatccag agaatcctga tcccaataaa tctgtcgatc catcagatcc
    22261 tccaagccct ggaacaggag gggcattatc gattgattat ggatcaaaat tcaaatttgg
    22321 gactcaaaaa atttctactg cggataaaac gtattatgca gctgctgatg tgatgaacga
    22381 tgggtctaga aaaccgacat atgtccaagt gacagatagg agaagtacct tatctgggtg
    22441 gaaattaagt gtctctcaac cagaacagtt taaaactgcc agtggggatg aactagttgg
    22501 agcacagctg aaatttacga aagggcaggc tgtttctttg gttgatccaa catatacgcc
    22561 gcaaacggtg aattctgaat taacgttgac accgggagga aacaatacgt tagcaattaa
    22621 tgctaaatca ggaacaggtg tggggacttg ggtctatcga tttggtgcaa atgaaaatga
    22681 aaatcaagat gcggtacagc tttcggtacc cggtaaatct gtcaagttag cgcaacaata
    22741 ttccactaaa ttagtttgga cattagaaga tacacctaac aactagttta ctgaaaagaa
    22801 ctgttaaaaa taaatacgta gtcatattgt cttgcgaggt aatcgagcaa aaattgtcaa
    22861 aggctaggct aaatttgttt ggatacgaat agttcatcta ggaatttaac gatcaggagg
    22921 aaaagatata ttgaaaaaga ggaggatcgt gctcatttta agcagtctct tagtgactaa
    22981 tatctttccg atcatagggt actctttgga attgaatcag ttggagcatg gaaatagaag
    23041 agacgaagaa agcaaactca cttcgacatt cgacagttta atagaatcca accagacaca
    23101 agaaagcagt gaggatttaa gtatttcgag tagtggagag accactgaca acgtagaagc
    23161 tgagggttcg gacaaacaag gactagaaga tgcaccaatt tctgcggttg agctgcctga
    23221 cgatcccaat attatttcta tcgataaaat ttttcaaaca cctatcggta ctagcacgtc
    23281 catattggaa aatgggaagt tactgcagtt gaacccagca gaaaggtctc aaagaggagc
    23341 tatttggtca aataagaaga ttagtttact ctcggatttt acatttaaat cttatttata
    23401 cttaggaaat caacgagata gagcgggaga tggcatgacc tttacattga ctggcgatcc
    23461 tagaatggct agtaatccca gtcaagttat tggatcgcca ggtatgggaa ttggctcata
    23521 ttctactcgt tctggccaac cttacattgc gaatgcgctc tcgattgagt ttgatacgta
    23581 taaaaatagt ggttcttctg atcgaatgga tcgagagata actgctgata atagagggca
    23641 tggacatgtc gcttttgtca cgcctaaacc aaataataat aattattctg gagagcatac
    23701 tggcgtgacg gtagctccta catttctttc taatggcaca tggcgcttgt taataattaa
    23761 atgggacgcg gagcaaagaa gtcttactta tgatttggaa ggtgtcggtc gctcttctcg
    23821 gattatacct gatctcatta ggcagtttgg ttctacagaa gtaaattggg ggtttacttc
    23881 ttcaacaggt actttttttc aagaaaatgc tgtcgctgtc acgcgacttc catcgaatgt
    23941 cacatcaaca gctgatgtaa aagtcaacga tagtgattat ggggccatcg ctgaagctgg
    24001 aggaaacgat aagataacta tcagaaatac attgaaggtc gagggggatt ttcttgaata
    24061 taagaaaatg cagactagta tcgacctccc acaagaatta actattcccg agcatatctt
    24121 gatcaatgga aatcaaattg atgccaaaga tatcttgata accggaaatc gagtcacgct
    24181 aaatctgaca gattatttta ttgctggaga tacgttaata atcgaattgg agacaacttt
    24241 gcaggaagta attcctgaaa aagtactcac tatgcatttt gaataccttg aaaacgggaa
    24301 tttgattgag caatcgaatg aaataaacct aaagatcgca aagctgaagg agatagatat
    24361 ccatgtttat tataaagata aagacagtca gattgatatt gctgaaatga aaacaatcac
    24421 tgggaaaata ggagagaagt ataaagaaac tcctattgat attgaagggt atgtttttga
    24481 ctcggattca gcgaatacag aaggcatctt ctccgaaaat acagaggata tctattttta
    24541 ttacagaaaa ggcaaacttt atctctcaac tgctcctcaa tatgtcaatt ttggaaaaca
    24601 taaaatatcc agtagaccat taagtgaatt cggacagttt tcaggtgggt tgaaaattat
    24661 agatgaacgg gcttttggaa gttggaaatt acaattgaaa caagagaaag ttcttacaaa
    24721 tggagatttt gagcttccag aagctctttc ctttgttacc tctgaaaata caacggttat
    24781 agggacatcc gctgttacga ttttcgaaag tgatcaaaag ggtgaaagcg atttgtcctc
    24841 tcttttagat tctacagctc atcgtggaat cagaataaat attcctgtag aaaaccaaag
    24901 aatcggaacc tttgagggga aactatcttg gatacttgca gatgtcccag gaaattaaca
    24961 ggaagagaag aatataacaa aattattgag tttcaagaag ggcacaggta ccataggctc
    25021 ttgtgtccca cagagacaag attttgttat agtcttctct tacatatgat taaaggagcg
    25081 attttcttga gaccaaaaca acaattattt ataacactcc tagttatttt aggggcactt
    25141 tttaacaata tatcagtgtc agctagtgca tttaattttt cagtgacgcc gataacttca
    25201 gagaatcaaa tcgacaagag aaaaacttac ttcgatcttc aacttgatcc ggatcaggaa
    25261 gtagaagtaa aagcggaatt gagaaatgat acagaaaaag aagtgaaaat cgatatttct
    25321 gtgaatagcg caaccaccaa ttcaaatgtg atggttgaat acggaaaaaa cgagattgaa
    25381 aaagacatga gcctgatctt tgatctggta gattatgtga gctatccaca gatagtgacg
    25441 ctaaaaccta aaagtgtcca aaccgttgct tttcatgtca ggatgccgaa tgagcgattt
    25501 gatggtgtat tggctggtgg gatcacattc caagaaatcc agacggaaaa ggaccaaaca
    25561 gaaactaagg atcaaagctt atcgattgag aatgaataca cctatattat tgctttgttg
    25621 atgcaacaga cactgaacga agttgctccg aaccttactc ttcatgaagt taaaccagat
    25681 caaatcaatg caagaaatgt catcttagca aacgttcaaa atgatcaaaa aacctatatc
    25741 aatcaagtag ttattgaaac aaaaattaca aaaaaaggac attctgaagt tttatatcaa
    25801 gaggaaaaag aaggattgca gattgcgccg aatacaaatt tttcttttcc aactgcttta
    25861 aatggtcaac cgctaacccc aggtgaatat caccttacaa tgactgtttt aggaaatgaa
    25921 aatgaaaatg gaaaattttc gagaaaaaaa ggaaatacta cgatcaacta taccaatcaa
    25981 tgggtatttg aaaaagattt tacaatagac ggaaaagtag ccaaggaatt gaacacgacg
    26041 gatgttacga ttaaaaaaga caataattgg atttatatct tagtagcttc acttttctta
    26101 ttgtttattt tattatgcat acttttgttt gttttgagaa aaaaagaaga gaaagaaaag
    26161 taaggttaag ataacaaata gtaaattttt attattgtag gatggttggg ctctttttat
    26221 aacaatataa aatataccat tttattaata aaaatgcata ctagtaaatg atataattct
    26281 aataacaggt ttgatcgcga gaaaggccaa ataaaacgct ctccaaaatg ttaaaaaaaa
    26341 gacaggacga tgtatattat acaaacaaga atcgctgaca acagtgactc ggatccagga
    26401 aatcagtttt atccccagct agttggtgaa tgcatggaaa gtaatatacg tagaaagagc
    26461 tgatgactaa tgaattatga gtttttttta agcaaagaag atcacagaag ttattcatta
    26521 cttaaatatt tagaagcaag ctcaaattta tcagagtcaa tatcgaatgt tcaagaagag
    26581 ttgtcgctat cgacatttct cttgaaaaaa acgatcgata agctgaaaca tgatttacaa
    26641 aagttcgatc tagagaaaaa tttgcgagta accgtcagtg aattggatat atgtctggaa
    26701 attaatggaa aatgttctag caaagctctg ttatctcgtt atataacgga ttctttatct
    26761 atgaaaatgg tattggcttt ctttcaggaa aaatatatct cagtagaaaa ttttgctaaa
    26821 cagcatcacg tgagttattc tgtggcttat aaagtattac aaagactaaa aagaaatcta
    26881 aaaaaatatc aaattttttt tgaaaagaga aagtt
//
LOCUS       DAYYHS010000034        26429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000034 DAYYHS010000000
VERSION     DAYYHS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.034"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..631)
                     /locus_tag="ACHA6A_001389"
     CDS             complement(107..631)
                     /locus_tag="ACHA6A_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8969595.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACHA6A_001390"
     CDS             complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACHA6A_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8969596.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACHA6A_001391"
     CDS             complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACHA6A_001391"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8969597.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2353..3033)
                     /locus_tag="ACHA6A_001392"
     CDS             complement(2353..3033)
                     /locus_tag="ACHA6A_001392"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8969598.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3159..4028
                     /locus_tag="ACHA6A_001393"
     CDS             3159..4028
                     /locus_tag="ACHA6A_001393"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8969599.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4054..4569
                     /locus_tag="ACHA6A_001394"
     CDS             4054..4569
                     /locus_tag="ACHA6A_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8969600.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4616..5179)
                     /locus_tag="ACHA6A_001395"
     CDS             complement(4616..5179)
                     /locus_tag="ACHA6A_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8969601.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5234..5413)
                     /locus_tag="ACHA6A_001396"
     CDS             complement(5234..5413)
                     /locus_tag="ACHA6A_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969602.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5490..5999)
                     /locus_tag="ACHA6A_001397"
     CDS             complement(5490..5999)
                     /locus_tag="ACHA6A_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969603.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6095..6475)
                     /locus_tag="ACHA6A_001398"
     CDS             complement(6095..6475)
                     /locus_tag="ACHA6A_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8969604.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6805..7554
                     /gene="pnuC"
                     /locus_tag="ACHA6A_001399"
     CDS             6805..7554
                     /gene="pnuC"
                     /locus_tag="ACHA6A_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8969605.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7738..9015)
                     /locus_tag="ACHA6A_001400"
     CDS             complement(7738..9015)
                     /locus_tag="ACHA6A_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969606.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9094..9738)
                     /gene="pcp"
                     /locus_tag="ACHA6A_001401"
     CDS             complement(9094..9738)
                     /gene="pcp"
                     /locus_tag="ACHA6A_001401"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8969607.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9937..10881)
                     /locus_tag="ACHA6A_001402"
     CDS             complement(9937..10881)
                     /locus_tag="ACHA6A_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8969608.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10883..11569)
                     /locus_tag="ACHA6A_001403"
     CDS             complement(10883..11569)
                     /locus_tag="ACHA6A_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8969609.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12043..12363
                     /locus_tag="ACHA6A_001404"
     CDS             12043..12363
                     /locus_tag="ACHA6A_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8969610.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12517..13422
                     /locus_tag="ACHA6A_001405"
     CDS             12517..13422
                     /locus_tag="ACHA6A_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969611.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13419..14192
                     /locus_tag="ACHA6A_001406"
     CDS             13419..14192
                     /locus_tag="ACHA6A_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8969612.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14373..15797)
                     /locus_tag="ACHA6A_001407"
     CDS             complement(14373..15797)
                     /locus_tag="ACHA6A_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8969613.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16008..16454
                     /gene="nrdI"
                     /locus_tag="ACHA6A_001408"
     CDS             16008..16454
                     /gene="nrdI"
                     /locus_tag="ACHA6A_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8969614.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16519..18501)
                     /locus_tag="ACHA6A_001409"
     CDS             complement(16519..18501)
                     /locus_tag="ACHA6A_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8969615.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18515..19411)
                     /gene="murQ"
                     /locus_tag="ACHA6A_001410"
     CDS             complement(18515..19411)
                     /gene="murQ"
                     /locus_tag="ACHA6A_001410"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8969616.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19414..20475)
                     /locus_tag="ACHA6A_001411"
     CDS             complement(19414..20475)
                     /locus_tag="ACHA6A_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8969617.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20686..21213)
                     /locus_tag="ACHA6A_001412"
     CDS             complement(20686..21213)
                     /locus_tag="ACHA6A_001412"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8969618.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21280..22782)
                     /gene="eat(A)"
                     /locus_tag="ACHA6A_001413"
     CDS             complement(21280..22782)
                     /gene="eat(A)"
                     /locus_tag="ACHA6A_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8969619.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23040..24161)
                     /locus_tag="ACHA6A_001414"
     CDS             complement(23040..24161)
                     /locus_tag="ACHA6A_001414"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8969620.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24287..25228
                     /locus_tag="ACHA6A_001415"
     CDS             24287..25228
                     /locus_tag="ACHA6A_001415"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8969621.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25378..26274)
                     /locus_tag="ACHA6A_001416"
     CDS             complement(25378..26274)
                     /locus_tag="ACHA6A_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8969622.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
BASE COUNT         7629 a         5581 c         4669 g         8550 t
ORIGIN      
        1 tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc
       61 tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat
      121 aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga
      181 ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt
      241 gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt
      301 ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt
      361 ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg
      421 tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact
      481 cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg
      541 tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt
      601 cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc
      661 tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa
      721 tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat
      781 agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa
      841 tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga
      901 ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct
      961 tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga
     1021 ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat
     1081 cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg
     1141 cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg
     1201 gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt
     1261 tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt
     1321 gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat
     1381 ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg
     1441 gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga
     1501 tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt
     1561 ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat
     1621 cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg
     1681 gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca
     1741 tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg
     1801 ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca
     1861 tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt
     1921 tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt
     1981 aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat
     2041 ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc
     2101 gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac
     2161 ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt
     2221 ttatatgtat tatcatacac tattccagaa aaaaataaca gtttatttta tctgtatcag
     2281 tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa
     2341 agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc
     2401 aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga
     2461 taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat
     2521 tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa
     2581 tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg
     2641 cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac
     2701 tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt
     2761 tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg
     2821 attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt
     2881 caattccaat gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc
     2941 tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac
     3001 ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata
     3061 actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt
     3121 aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag
     3181 atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga
     3241 tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag
     3301 aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg
     3361 cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag
     3421 caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg
     3481 atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta
     3541 cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt
     3601 tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag
     3661 ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc
     3721 agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga
     3781 accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt
     3841 ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag
     3901 caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata
     3961 caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag
     4021 acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag
     4081 cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg
     4141 atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat
     4201 ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa
     4261 gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat
     4321 attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat
     4381 catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat
     4441 gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta
     4501 gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac
     4561 ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga
     4621 tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga
     4681 agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga
     4741 tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga
     4801 tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa
     4861 acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag
     4921 gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg
     4981 caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat
     5041 cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg
     5101 gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt
     5161 ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga
     5221 aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag
     5281 taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat
     5341 tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt
     5401 tcattttttc catgttatct caacttcctt tcatctttac tctcttagct tactcttata
     5461 ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt
     5521 tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac
     5581 tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt
     5641 tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca
     5701 tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa
     5761 aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga
     5821 atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat
     5881 gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc
     5941 gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt
     6001 cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga
     6061 atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc
     6121 cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt
     6181 ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga
     6241 aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa
     6301 ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt
     6361 ctttgcggta attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg
     6421 atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag
     6481 cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg
     6541 tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa
     6601 agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt aagaaattat
     6661 attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat tgaaagacta
     6721 gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac acactacatt
     6781 tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc tcatcagcta
     6841 aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg ccaggtcatg
     6901 actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg taccacgctt
     6961 ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat tttaggtatt
     7021 ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt aagtatcggt
     7081 gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa agaatggaat
     7141 cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat cgcaatcatc
     7201 agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac agatgatcct
     7261 cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat catcagtttc
     7321 ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca aatggtcctt
     7381 tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa cagctctgtt
     7441 tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca aaaagaacgt
     7501 gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc ataaaaaaac
     7561 tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     7621 ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
     7681 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct ctatttttta
     7741 tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt
     7801 ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca
     7861 tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt
     7921 ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg
     7981 caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt
     8041 aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg
     8101 ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt
     8161 ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc
     8221 atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc
     8281 tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt
     8341 ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata
     8401 atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt
     8461 tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt
     8521 ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa
     8581 ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg
     8641 caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt
     8701 aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg
     8761 ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa
     8821 ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc
     8881 ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt
     8941 agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga
     9001 ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat
     9061 aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga
     9121 aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc
     9181 ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg
     9241 cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg
     9301 atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc
     9361 atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg
     9421 accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc
     9481 ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc
     9541 gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct
     9601 tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg
     9661 catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc
     9721 tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt
     9781 ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa
     9841 cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg
     9901 ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc
     9961 aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt
    10021 gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag
    10081 cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc
    10141 atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc
    10201 attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt
    10261 cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc
    10321 gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac
    10381 tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa
    10441 gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa
    10501 attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat
    10561 tttcattcct agtttgtctg acattttttc catatattct tttgatggaa gccggctttc
    10621 tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt
    10681 cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa
    10741 ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat
    10801 caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc
    10861 aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt
    10921 tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa
    10981 cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt
    11041 ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat
    11101 tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc
    11161 cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa
    11221 ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta
    11281 caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca
    11341 gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc
    11401 gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag
    11461 tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa
    11521 acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc
    11581 cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt
    11641 ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttatcac aaaaattatc
    11701 ttacttattg taaggtaaag ctttttttaa tttattgccc gataatatat agaaataagg
    11761 tctagttact gtttttttta aatgattaat aaataacaac ttctttctat gaaattgcaa
    11821 agcattcatc ttcttttccg ctaacgatat atccgtcagt gcttggtaca ctttactaaa
    11881 aagtatgaga ttgccgatat ctggataggt gtgctatact acagaaaaag atatacaatc
    11941 tgccaccggg taggataaag aggatgatca ttccattagg tctttgaagg gatgattttc
    12001 ctctttttgt gtattttttt atagttaagg aggaatgtaa gtatggcatg gatggaatta
    12061 atcatagcag gaattttgga agtattctgg tcaacgatga tgaaatggag tgatggtttt
    12121 tctaaaatca attattcttt ttacacagtg attggcatga ttgctagttt ttactttttg
    12181 tcaaaggcaa tcaaaagttt gccgatgagt ctctcttatc ctatctggac agggattggc
    12241 gcagttggtt cggtcataat tggtgtggtc ttgtttcatg ataagctgaa tctttccact
    12301 tggttctttg tggggatgct actcatcagt atcattggca tcaagattac tagcggccac
    12361 tagacacttg gaataagtac tgatcagaaa aaataagcct ttttatttaa tattcaattt
    12421 ttttatgaaa aaataagaaa taagtccaat gacctagcga cttctccact catcaggtgt
    12481 atgcttgtac tatctataga aaaggagtga atatttttga aaagtctttc ggtccaagaa
    12541 ttatccaaga actttggtca caaacaagcc ctcagcgatg tcagttttag cttatcccct
    12601 ggcaaaatcg ttggattagt aggtcctaat ggcgctggaa aaacaacgat catgaaagct
    12661 attttaggac tgatccatta ctcagatgga acaatctgta ttggtgaaaa tgagatttct
    12721 ccctctagtc ataaagggtt ggaacaagta ggagcattga tcgaataccc gggtatctat
    12781 ccattcttaa caggctatga tcatctgaag ttattctctg aaaccaacga tgtatctgct
    12841 atcgacacta tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa agcgaaatcc
    12901 tactcgttgg gaatgaaaca aaaattggga attgctcttg ccttactgaa ccatccgtct
    12961 atcgttattt tagatgagcc gatgaacggt ttggatccac aagcaacaag agatgtgcga
    13021 aaaatcatta ccgacttggc tactcagggc acctctttct tgatctccag tcacttactc
    13081 agcgagcttg aaaaaatggt cgatgagctg atcctgatca atcaaggaaa gatcatcaaa
    13141 caatgtacaa tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat ctctacaaca
    13201 aatgatctag ctgctcgaac gatcttgaca gaagctggat atactttgga agatcctgat
    13261 aaaatcaagc ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca gctattgtct
    13321 gcacaaaata tccatgtgac agacgtccag catctgacga atgatttgga atcttctcta
    13381 ttggaaattc tggattcagt aaagggggaa gaagaaaaat gacattgctt gtaaaacaag
    13441 aattatttaa attgatcaag aaaaagtcaa ctgctgtttt atctgttcta ttagttgttt
    13501 tgctcatagg aacagcactt ctagccaaaa aatacacgac gatcattgat ccagttgaaa
    13561 tgactgctca gctttttagt gccacatctt ggattgtctt tatcatgatt gcagctgcaa
    13621 gcacgatcat ttcgatggaa gcacaatatg gtacattgaa aaatctcctt tatcgaaagt
    13681 attctcgagg agaaatatta gtaagcaaat ggatcacatt agttatttat tctgtttatt
    13741 tatacctttt agctattatt gtcactgtgt tgatgaaact gattcttttc ccttcgattt
    13801 ccttcacgga gaaggtttct actggtcaaa cactgatcca aagcttgttc acttatactt
    13861 tgggtagtta tatcggttta tggctgattt taagtttagt gttgatgatt gcttgcttta
    13921 tcaatagttc cggcgcatcc atctcagcag gaatcgtctt ttattttgct tcgtcaatca
    13981 tatctggtat ccttattgca ctcatccaaa aatgggagtg gatcaaatgg aatccgatca
    14041 gtatgttgaa tctgcaaaac caaattggaa acgaagagat catgaagcaa ttgactcatc
    14101 tttctactaa tcaaatgcta ttcggtaatt tagcttacat cgttttgttc ttagcattag
    14161 gatatctcgt atttaagcga aaaaatgtat gatgactcat caaaaaagag gctgtgaaag
    14221 agacgttcag ccttattgcc gattggctgt ctcttaaaca ccgtttattc tgaaaaagga
    14281 gctgtgacaa catttgtgtc acagctcctt tcctagtatg tttttgaata aacgacaggc
    14341 tcacaacctc tctttatact tcttttatac ttctatacgt gaagcatccc agtggcaaat
    14401 aaaaccattg caatcactcc gaccatcgtc acacctataa gtaaaatctt atctaagtgg
    14461 atatgttgta caggctcgtt ttgctcctta cgtgctttga ggtaaaaata aatgcctggc
    14521 aagaaaagaa caagaacaag cattacttca ttaaaggcta cgactagcat agcccatact
    14581 tgaaaggcac ttgctaaaag ccctactaaa aattggaccc attctttccg ctgataagaa
    14641 agtttcattt ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc tgtaccaagt
    14701 gtgtaagcaa agttataagc actgtcagtg aataataatg aaagtaaaaa gacattcgta
    14761 cataacgtcg tcaatacgat agcataagtt ggtgccccat ttttgttgag ttttccccat
    14821 gctttaggca aaagtccgtc ttcagccatg tttcccattg tttcaatcgg taagatcgtc
    14881 catgagagcc aaacaccaac taaagaaatg atcaaaccgc cgttgatcac gactgctccc
    14941 catttaccaa ccatcatttc aaacacatag cccatagcag gctgcgcctt gatcgtttgc
    15001 agctgattca tcgtcattgt cccatatgga agcatggaaa ccaagacgta aataataatc
    15061 aagccgaata tccctagaat cgttgctttc ccaacatctg attgtttttt tgctcgtgaa
    15121 cctacgacag aggctccttc aataccaaca aatacccaaa gcatcaccat gatgcaactc
    15181 aatacttgac tggaaaacgg gatcggctgt ccattcgcat tcaatataat gtcgctagaa
    15241 aagttatcaa tgaattgttg gatcgaaaac gtgctgatag ctactacaat aaataaaaat
    15301 aatggcacta atttacagaa tgtgacgata gcattgacga aactagcatt ttcgactccc
    15361 cgattcacta gccatgcata tccccataaa aagactgtac tcatcacgat cgatgccaga
    15421 ttttgcccac cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa caatgttgca
    15481 aaagctacgt tgcctaacca gcatgtgagc cagtaacccc aaccgctgat aaaaccagcg
    15541 aaacggccaa aaccttcttc tgcgtaacta aagatcccgc cttttaaatc tggacgtttt
    15601 ttattgatat tattgatcga caaggagagc atcaacattg ctgttcctac gagtacccaa
    15661 ctgaaaatag ctgggccgcc agccgatccg gagatgtccg tcataatacc aaatacgcca
    15721 cctccaatcg ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat tccttttttc
    15781 tgttgctctg tatccatgta agcatcctcc aaaataaaac cgcttttatc aaaaagtata
    15841 acaaaccttt tttattttta cttaattaat aaaaacttta atagaaatat aattctaccg
    15901 gcttttttct cattatacaa gcaaagcgcc tagtcccttt ccaaattaca ccaccaaaaa
    15961 acttaaaaaa gatgctatac taaaaggaaa gaattggagg atgatccatg aacattttat
    16021 atatatcgat ttcaggaaat acgcgtgcct ttgccaaaca tctagcagaa tatgctgaaa
    16081 aaatgcatgc agaagatcct gtcaatcctg aagttacttt aaaagaaatc catgaaaata
    16141 gtgattttac gaaagaaaca gaaccatttt ttactttcgt tcctacttat ttagacggcg
    16201 gaaatggttt agataacggt gatacagaaa tcttgaccga aacgatgcgt gaatatctgg
    16261 aacatgaaga caatcataaa ctttgtcttg gtgtagtcgg tagcggaaat aaaaacttca
    16321 ataatcagta ttgtctaaca gccaaacaat atgcacaaac atttggcttt cctttcttgg
    16381 cagattatga attacgggga acaccatctg atgtgacacg aatttatcag attttagcag
    16441 aaaacaataa ataataaaaa agaagaagct gaaaaataga tttcagtttc ttctttcgtt
    16501 tctattgttt ctatcatatt aagctttgac atccatcaca ggtgcttttg cttttgcttt
    16561 acctgttttt tccaagacaa agttttttac acgatccata ttggttacca agacgatcat
    16621 ggatgtttct ttgcctgctg ctttgatcgc ttctaagtca gcttctgcta caagcgtatc
    16681 aggtgtcact tgctgccctt ctgttacttt cacatcaaat ggcgtaccgc ctaagtcaac
    16741 tgtgttgatt cccatatgaa gcaagatttc taaaccattt ggcgttgtga tcccaatagc
    16801 gtgtttcgtt gggaagacag agctgactac cccagtaact ggagaataga tatgtccgtt
    16861 ttctggttct acaaagtagc cttcacccat catcttttgt gcaaaaactg gatcacttgc
    16921 tttatctaaa gaaacaactg ttccgtcagc aactgatgag aggatagtca cactttcagc
    16981 tgaagtttga ggtactactt tttcaggcac ttcatttact gtggctgttt cagtcgctaa
    17041 ttcttctttt tctaaacggt ctttaggaat accgaagaag aatgtagcaa cgaagccacc
    17101 agcataagct gccaataatc caaggatgta gcctaaccat ttaccgtcag agatcaatgg
    17161 gatcaaagcg gctcctgaag gtccaatagc gatagccccg acatttccga tcattccgat
    17221 cactgccccg ccgatacctc caccgataca agcagtaata aatgggcgtc ctagaggtaa
    17281 tgttactccg taaatcaatg gttcgccgat tcctaggatc ccgactggta aagctccttt
    17341 gatcatttct gttaattctt tatctttttt gcagcgaatc cataatgcca atgctgcacc
    17401 cacttgtcct gcaccagcca tcgcaagaat tggaagcaat agcgtacgac cagtttcatc
    17461 aatcatttgc acatggattg gagtcaaaat ttgatgcagt ccgaacataa ccatcggcaa
    17521 gaataatgtg cctagaacga agccggcaaa tgctccacca acgccaagta cccagttgat
    17581 cccaccgacc atgccatcag aaatgaaacc tgctaaaggc atgatgaaga aaatttcaat
    17641 cagaccgatg gcaatcaatg aaagagttgg tgtaacgatg atatctaccg catcaggtac
    17701 tactttatgc agttttttct caacgattga caatagccaa acagcgaaga tgacacctag
    17761 gatcccacct tgtccagctg caagagcttc accagtaaat aagtttttca gtggtgcttc
    17821 cggattcatc cctgtcagca tcacaacggc cccgataact ccaccaagag ttggtgttgc
    17881 accaaaaact tgtgcagcat tgatacctgt atagataact aggtagctaa acatcccgtt
    17941 tttcaagatg ttcatcacat cgatatattg ttgccatgtt gctgcatcaa gtgttccggc
    18001 tgtgactaag tttgctaaaa ctgcggcaat ccctgccacg atcccagtac cgacaaatgc
    18061 aggaatcatt ggcacaaaga tattagaaat gtcttttaat accgatttga ttttagatgg
    18121 tttttgtttc gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta cacggtcttt
    18181 tccagatgct gatgaatttg ctgtagctgt acttacagaa gaaagttttt ctcctaattt
    18241 cacgcctgcc aaatcaacca tttgctgtgc tactttgttc actttcccag gtccgatgat
    18301 gacttgaagc tgttcatctt ctactacacc aagcactcct ggaatcgctt ttaattcaga
    18361 gacatttaca agatcatcat tgatgatctt catacgcaca cgagtcatgc agtggacaat
    18421 gctttctaca ttgcccatgc cgcctgcccc tttataaatt tgttttgcca gttcttccaa
    18481 agtcagttcg ttttttgcca tggtcttttc cccctcataa ggtccctttt acaaatccgt
    18541 ttgcttcagc taattttgct tgagcttcct ctttatcact atttgtcaaa atcatgacga
    18601 tagccagctt aacattttga tctgcttctt caaagtactt ttcagctgtt tcgtaatcag
    18661 cttctgtcgc ttgcatgatg attcgttttg agcgttcgat tagtttttca tttgttggtt
    18721 tgacatcgac catcaagtta ttgaatactt tacctatacc aatcatactg atagtagaaa
    18781 tcatattcaa gataagtttt tgtgctgttc ctgacttcaa tcgagtagag cctgtcaaaa
    18841 attctggacc tgcatcgact tcgattggga aatccgcgtg tttactgatt tcggcttgtt
    18901 tattgcaaga aatcgttcct gtgacagcac cgatcgattt tgcgtaatct aatccaccga
    18961 tcacatatgg tgtacgaccg ctagctgcga taccaataac tatatcattt tttgtcaaat
    19021 tcagcttgat tagatcttct tgccctagct cataagaatc ttcggcccct tcgacagcta
    19081 cagtcattgc ttcttctcca ccagcaatta gacctatgac catttccggt tctacaccga
    19141 atgtcggtac gcattcagca gcatctaaca cacctagtct tccacttgtt ccggctccca
    19201 tataaatcag acgtccacct tttttaaagg actcaatcgt gttagcaatc actggttcga
    19261 tacttgtcag ttctttttca accgcagcgg ccactttctg atcttctttg ttcattagtt
    19321 gaacggcttg agaaatactc atctcatcta atccaaaagt ttcttggttt ctgcgttccg
    19381 ttgttaggtt ctctaaattg attttgctca tttttatctt ccttcttttc ttataaattg
    19441 atagttcata ccagctttga tttggttgat caatggtagc tcttctttga taactttagc
    19501 tactacattc actttttcat ccgctggcaa atcatatttg gttaattgga tctcacccat
    19561 atagcgcaaa tacttttcgt tatctaaagt aatagaccct ttcatacgcg tagctgtatt
    19621 tcttgctggg ataggcggaa tctctcgaaa acgtgcatct acgcttcgta tgacatctct
    19681 tgcatcgtct tgccgattcg tatgcttccc taatatatat tcgtaaaatt cttcatctac
    19741 aggttccgta tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt gctcctgaac
    19801 gttttctgac agacctgcat ctccgatgta aacaatatcc gtgttacttg ctaatagatc
    19861 tagagcgcct gcaagaggat ggatccctcg atgttcttcc aaagttggca atccttgata
    19921 tagtgggcct ctcaactctg tatctcctgg tacaaaacct tggacaagga gaccttgaat
    19981 tttgagccat tgattttttt tctgatacca ttctttgtcc agccctgttt cgggacgtgg
    20041 ataataatta tgccaagctt caatctgtga gaaatcagct tctgcttctt tcaattcatc
    20101 gatatcttta ggcgtgatcg tgctagcatt caaactgatt ttcaattgat gtgaccagtc
    20161 ggcaatctgc tgattggaaa tatgataatc cattcttaaa cctgttacac caattgcttt
    20221 caaaggcgtt agatcttcta atgaaaaacc cgctcgttcc aatgcctctc ctgaaatatc
    20281 gaccatcaat tctagtttat tctttttcgc ttcgcttcct agttcgatca atcgcttttg
    20341 atatgccgaa acatcatctt ctggtatatg catggaagtg aaaataccga caaagccatt
    20401 ggatgccatt tggcggatat actgtttctt ctcttctgac aaagaatcgt tcataaatat
    20461 tgaaaagcca aacatattat ccctcttttt ctgtatccgt cttcatttct atgactagag
    20521 tatatatcta aagaaacaaa atttcaatac ttttattaaa ataattaaat ttcatttcta
    20581 aaggttgagt ttatattatc aaaagaattg tcataaccta aaaaaggcac tgctaagcaa
    20641 aagcagtgcc ttgaaatatt ttatttaaat aacacaaaaa cataattact cataagaaac
    20701 caattctgta ttatctacca tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata
    20761 agcattgatc actcttatca aggcattatg gcaaactaac agatagacat tttcttcgtc
    20821 ttcgatgcat tctttcaata aaggaacgat ccgtgcatac acatctaaga ctgactcccc
    20881 atttgggaat cgtacagcga acgctaaacg tgctttttga aagttctcat ctttgcttgg
    20941 cagtccatca taatccccaa aatccatttc taccaatcgt tcatcaacgg tcagcggcaa
    21001 gctgagacga tcagcaactg cttgtgctgt atctcttgct cgttgaagag gggaatggat
    21061 gattttcgtg attggaacag gcaaatctac taccttttct gctagtttct gtgcctgcat
    21121 ctgtcctttt tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca
    21181 agttgtttct ccgtgtctcg ttacataaag catgtgattc tctccttcgt tggcaaagtt
    21241 ccccttaacc ataccttgtt gttcttgaga ttacaagggt tagattctct caagagaaat
    21301 aatctcagta gatactttat ccaggaaggt ttgatcatgt tccactagta acatggcagg
    21361 tttcacgttc aagatcagtt gttctaattg ttcttgattg aagacatcca aataattcaa
    21421 tggttcatcc catgtatata gttcagctgg ctgtgataaa gatttagcca attccacttt
    21481 tttccgttgg cccatactca tctgctcgat cttgttatga aaaacatctc tttccatccc
    21541 aagctttcgg aggttgttca aaaatgcttg gtagtctact tggtttttct ctgcaaattc
    21601 cgccaacgtt cctcgattgt cttcataatt ttggcttgca taactaatgc tcagatgttt
    21661 tggctggtat ttttctccta taaccttgcc gttgaatgcc cccagaagat aatggataat
    21721 ggatgacttt cctgcaccgt taggacctga aatcgccacc cgctgatgag gctcgattgt
    21781 aaaatgaatt ggctcgaata acagattttc ataccctaat tgaagatctt ctacgcttaa
    21841 aagtcgctta tggtgagacg cttggctatt catcgtcaaa gaatcgatat attcgatatc
    21901 ttttaatagt ttttctttct cgctgatctg ggtctccatc cgattcacga ttgctttgga
    21961 tcgtttcatc gtccgtgcag catcagcacc cactgctcct ttattcactc gtctagattc
    22021 agtatcgatg aatccgactt gtttctttgt tttatctcct tctcgggaac gagaccattc
    22081 ggctttttca gctgctgttt tctttagcct actgacttct tttttcaatt tttcattttg
    22141 agccatttca aattcatcac gaagtttttt ctgttcttca tagatagaga aattcccttg
    22201 ataaagttcc agttgacttt tttcgattgc taaaacatgg tccactactt catcgataaa
    22261 tccccggtca tggctgacca cgatgaagcc ttgttttttc tttttcaaat aagccgctac
    22321 ttgttttcta ccagagatat ccaaatgatt cgttggttca tcgattaacg ggaaatgagt
    22381 gtcatccaca aataaaagtg ccagtaggac ttttgtcttc tctccccccg atagtgtgct
    22441 gaattctctc cataagattt caggatctgt ttgcatcaat tggagctctc tttcgatttc
    22501 ccatatctca aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt ctttatcttt
    22561 tgtctgttgc gggaaatagg caaattcttg ctgatggatt acttgccctt gataaggtag
    22621 tttgttttgt agaatattca gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc
    22681 tagtttccat tgtgtgtcaa aatttagatt tgcttgttca aataataatg tgccttggct
    22741 gtcgtagccg aatgtcagat tttttatttc gattttagac attttccatc acctcgtaaa
    22801 aaaagaccag tcctattttc agaactggct aaagaggatg caagggttgg atacacacaa
    22861 aaagacccat acatcgaaaa aaccaagcca atcccgaaaa tctgcacact gcttccttgt
    22921 ctccacaagg tacagtctga atgcagcttt catcaatcga cttaattttt caatgcacgg
    22981 atccctcact ttgtcctata tttgaaatta cgatagcata aagaaaatga ttttgtcaac
    23041 tacttcggga ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt tgttttttct
    23101 aatgtttgcc tgtgttccat tgcttcttct aaacctattg gacccaaatg aatacttaag
    23161 acaattggga gaaaatcaga aagtccttct agctcttggt ctctacttcc gcaaatagcg
    23221 attattggtt ttcgatattt ttttgcaagc actgctacac cgtatggaac ttttccgtgg
    23281 atcgtttgct gatcgatccg cccttctcct gtaatgatga catctgcttg ttcgatcttt
    23341 ttttccaatc cagtgatttg gctgactaaa tcgaaaccag aaaccatctt cgctcccaac
    23401 aaaaaaaggg ctccaccaat accgcctgct gctccacttc ctggaatatc atctaaatcc
    23461 acctcccaga aatcttttat ttgctgagca atctttcctg cttgtttatc taagaaagct
    23521 agcgtcgtat ctattcctcc tttttgtctt ccaaattgcc aagcagctcc ttgttgacct
    23581 gtatatggat tggtaacatc tgcagcgata atcaatttta ctttagaaac aagaacgttt
    23641 cttcgatcaa aatgaagttt tttagtagat aataaaggat ttccttctgt gtacccttct
    23701 aaagaagcac ccaaggattg gagtaatcct agaccaccat cgctacaccc gcttcctcct
    23761 aaagtgacaa taatttgttc tacttcttga agcagtgcat ctttaataag ttctcctaag
    23821 gcataactgg aagcaagtcg gatagtttga tccgttggtt tatcaagtag atctaacccc
    23881 aatgcttgtg cagattcgat gactgcggtg ttttttcctt gccaagttgt caataaatag
    23941 ctgatttttc gctgacggaa taagagatcc atcccatcaa tggtacgcca tgttcccctg
    24001 agcgcgtaat agattgcttc tgcagtattt tctccgccat ctgcaatcgg cacattgatt
    24061 tttcttcctt tacgatcttc ccagccattt agaactgcct gatttagttc aaatgaagaa
    24121 gcacttcctt taaatgaatc aattgccgcc aaaatatcca tctgtatccc tcactttctt
    24181 cttaagtaag acactttttt tcattttttt caaacaaaac aatgctattt tcttccattt
    24241 ttctatatac taaaagggaa ctcattgaat aaaggagaga aaaaacatga aaatcgcaat
    24301 agctggggcc ggagcaatgg gcagccgaat cggattgatg ctacatcaaa gcggaaatga
    24361 ggtcctcttg atcgatcgtt ggcctgctca cattgaagcc attcgtacaa atgggttgat
    24421 tgctgacttt aacggtaaag aagtggtcgc aaaactgcca atctattcac cagaagaaat
    24481 catcgaaagc aacgaacacg tggacttgat cgtagcatta acaaaagcca atcaattaga
    24541 tgatatgttt tgttcgatcc aatccattat tactgacaat acttatgttt tgtgtttact
    24601 aaatggatta ggtcatgaag atgtactgga aaaatatgtg ccaaagaaaa atatcttatt
    24661 tgggatcacg atgtggacag ctggtctagc tggtcccgga aaagtcacat tgttaggtga
    24721 tggagaaatc gaactagaga acttggaacc agaaggcgaa gcatttacaa aaaaagttgt
    24781 agaagtcttt caagaagcaa atttaaatcc gatctatagc cataacgttc gttattccat
    24841 ttggcgaaaa gcttgtgtaa atggaacatt gaatggttta tgcacgattt tggattgtaa
    24901 tattgctgaa ctaggcgcgc aaaaagcggc agagagcatg gttcgaacaa tcgtatctga
    24961 atttgcttct atcgcagcaa aagaaggaat catactagat caagaagaag tgtaccaaca
    25021 tattgcttct acttatgatc ctgacaacat cgggttgcat tacccatcta tgtaccaaga
    25081 cctgatcaaa aaccatcgtt tgactgaaat cgactacatc aatggtgcca tttggcgaaa
    25141 aggacaaaaa tatgatattg ctacaccata ttgtgccttt ttgacccaat tagtccatgc
    25201 aaaagaagga atccttggcg cagaataaaa ggctctctga tgagaatcag ctgaatcagc
    25261 agctggttct ctttttttaa gcccaataaa agatcaaaaa gaaaacacaa ctctcatgat
    25321 gccataagaa ttgtgcttgt ttgtttcagt acataatctc ttcaaaatgt aggtttttta
    25381 tttgttttca acagaggaga ggtcatattc gtattgctca taaatagtgg aatcgttagt
    25441 gataaatcgt tttgcccaat tttctgcatc ataaaattcc aatagctctg ataggattgg
    25501 cttaaatggt gtatgcagaa attccggtaa ttcatctaaa tcacgttctc gaagcaaaaa
    25561 aatattttca ggacgataaa aatgataatg agcaatcgct tgattatccg tcactagttt
    25621 cttttgaaaa tacatggatt caaatgtccg caaagtcact ccttgctgac cctcccgcaa
    25681 cagttccaga atcccgcgcg attcccctgt ttttttcagg tactcttcat aagtaagata
    25741 tccaggttgt tcattccctc gatgtggaag aatgaaatag ttggtcgtaa catctctttt
    25801 tataaattcc tgcttatagc tttctgctcg ttcttttctc ccattattcg ttgcaccaaa
    25861 aaatagatcc gtctttggtt cgctagaaga aaaattcata tgtttcgaat aaaatgaact
    25921 attatgtttc agcccaagtt cttttgcttc caaaaaatca aaatgataaa tctcatcgat
    25981 tgccggttca tcttgtgcct gcttcagcat cgcctcgtgt tctgctttga agtgattcca
    26041 aaaaaataac acaatttttg cattggtaaa tgggcgcacg cgtcgagcca tctccatatc
    26101 aaatccataa tctaaaaaaa cgatcagctg ataatttgcc aactgttgtc tagctgattt
    26161 aatgaattcc gactgtagta aatcttttcc ggaccaaaca tctaaagagc ggatggaagg
    26221 caaaaaataa agtgatttat tttttgcaac tagcagtacg tcatttttgt tcatcttttc
    26281 cgcctcactt tctctgtcac tttttcctat gatagcctgt ttaaatagta aaaagccagt
    26341 aaaacgtttc ctatatttgt atgaaaattt aacatttgta tgaaaatatg atgaaataaa
    26401 tcattcttct attaaaattc gcctaaaag
//
LOCUS       DAYYHS010000035        26358 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000035 DAYYHS010000000
VERSION     DAYYHS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.035"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1552)
                     /gene="zwf"
                     /locus_tag="ACHA6A_001417"
     CDS             complement(26..1552)
                     /gene="zwf"
                     /locus_tag="ACHA6A_001417"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8969623.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(1567..2214)
                     /locus_tag="ACHA6A_001418"
     CDS             complement(1567..2214)
                     /locus_tag="ACHA6A_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8969624.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(2398..2727)
                     /locus_tag="ACHA6A_001419"
     CDS             complement(2398..2727)
                     /locus_tag="ACHA6A_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8969625.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            2904..3632
                     /gene="sfsA"
                     /locus_tag="ACHA6A_001420"
     CDS             2904..3632
                     /gene="sfsA"
                     /locus_tag="ACHA6A_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8969626.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            3648..4661
                     /locus_tag="ACHA6A_001421"
     CDS             3648..4661
                     /locus_tag="ACHA6A_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8969627.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            4661..5938
                     /locus_tag="ACHA6A_001422"
     CDS             4661..5938
                     /locus_tag="ACHA6A_001422"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8969628.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            6001..8703
                     /locus_tag="ACHA6A_001423"
     CDS             6001..8703
                     /locus_tag="ACHA6A_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8969629.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            8855..9172
                     /locus_tag="ACHA6A_001424"
     CDS             8855..9172
                     /locus_tag="ACHA6A_001424"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8969630.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            9202..9522
                     /locus_tag="ACHA6A_001425"
     CDS             9202..9522
                     /locus_tag="ACHA6A_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8969631.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            9630..11090
                     /locus_tag="ACHA6A_001426"
     CDS             9630..11090
                     /locus_tag="ACHA6A_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8969632.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            11158..11379
                     /locus_tag="ACHA6A_001427"
     CDS             11158..11379
                     /locus_tag="ACHA6A_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969633.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            11410..11592
                     /locus_tag="ACHA6A_001428"
     CDS             11410..11592
                     /locus_tag="ACHA6A_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8969634.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            11592..12005
                     /locus_tag="ACHA6A_001429"
     CDS             11592..12005
                     /locus_tag="ACHA6A_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8969635.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            12128..13309
                     /locus_tag="ACHA6A_001430"
     CDS             12128..13309
                     /locus_tag="ACHA6A_001430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8969636.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(13380..13544)
                     /locus_tag="ACHA6A_001431"
     CDS             complement(13380..13544)
                     /locus_tag="ACHA6A_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969637.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            13698..13771
                     /locus_tag="ACHA6A_001432"
     tRNA            13698..13771
                     /locus_tag="ACHA6A_001432"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13732..13734,aa:Arg,seq:cct)
     gene            complement(13840..14979)
                     /locus_tag="ACHA6A_001433"
     CDS             complement(13840..14979)
                     /locus_tag="ACHA6A_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8969638.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(15278..15913)
                     /locus_tag="ACHA6A_001434"
     CDS             complement(15278..15913)
                     /locus_tag="ACHA6A_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969639.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(16026..16661)
                     /locus_tag="ACHA6A_001435"
     CDS             complement(16026..16661)
                     /locus_tag="ACHA6A_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8969640.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(16695..17156)
                     /locus_tag="ACHA6A_001436"
     CDS             complement(16695..17156)
                     /locus_tag="ACHA6A_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM8969641.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(17286..17717)
                     /locus_tag="ACHA6A_001437"
     CDS             complement(17286..17717)
                     /locus_tag="ACHA6A_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8969642.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(17735..18055)
                     /locus_tag="ACHA6A_001438"
     CDS             complement(17735..18055)
                     /locus_tag="ACHA6A_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8969643.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            18354..19130
                     /locus_tag="ACHA6A_001439"
     CDS             18354..19130
                     /locus_tag="ACHA6A_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8969644.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            19145..19348
                     /locus_tag="ACHA6A_001440"
     CDS             19145..19348
                     /locus_tag="ACHA6A_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969645.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            19364..19702
                     /locus_tag="ACHA6A_001441"
     CDS             19364..19702
                     /locus_tag="ACHA6A_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8969646.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            19689..19868
                     /locus_tag="ACHA6A_001442"
     CDS             19689..19868
                     /locus_tag="ACHA6A_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969647.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(19911..20381)
                     /locus_tag="ACHA6A_001443"
     CDS             complement(19911..20381)
                     /locus_tag="ACHA6A_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969648.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            20468..21166
                     /locus_tag="ACHA6A_001444"
     CDS             20468..21166
                     /locus_tag="ACHA6A_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8969649.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            21344..21685
                     /locus_tag="ACHA6A_001445"
     CDS             21344..21685
                     /locus_tag="ACHA6A_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969650.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            21678..22349
                     /locus_tag="ACHA6A_001446"
     CDS             21678..22349
                     /locus_tag="ACHA6A_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8969651.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            22355..23047
                     /locus_tag="ACHA6A_001447"
     CDS             22355..23047
                     /locus_tag="ACHA6A_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8969652.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            23044..23793
                     /locus_tag="ACHA6A_001448"
     CDS             23044..23793
                     /locus_tag="ACHA6A_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969653.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            23805..24074
                     /locus_tag="ACHA6A_001449"
     CDS             23805..24074
                     /locus_tag="ACHA6A_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969654.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            24080..24244
                     /locus_tag="ACHA6A_001450"
     CDS             24080..24244
                     /locus_tag="ACHA6A_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969655.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            24237..24536
                     /locus_tag="ACHA6A_001451"
     CDS             24237..24536
                     /locus_tag="ACHA6A_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969656.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            24536..24838
                     /locus_tag="ACHA6A_001452"
     CDS             24536..24838
                     /locus_tag="ACHA6A_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8969657.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            24878..25243
                     /locus_tag="ACHA6A_001453"
     CDS             24878..25243
                     /locus_tag="ACHA6A_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969658.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            25733..25873
                     /locus_tag="ACHA6A_001454"
     CDS             25733..25873
                     /locus_tag="ACHA6A_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969659.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            25870..26052
                     /locus_tag="ACHA6A_001455"
     CDS             25870..26052
                     /locus_tag="ACHA6A_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969660.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            26065..26259
                     /locus_tag="ACHA6A_001456"
     CDS             26065..26259
                     /locus_tag="ACHA6A_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969661.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         8748 a         4404 c         5170 g         8036 t
ORIGIN      
        1 ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca cgataccagt
       61 tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa gctgctttag
      121 gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc caagcttttc
      181 gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag aagttcgttc
      241 catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta ttttcaacca
      301 tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct tgccctactt
      361 ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc gttagaacat
      421 taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat acattaacag
      481 gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt ttgcctgtac
      541 gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg cctgcgacga
      601 atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc ttgaattgta
      661 caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac acttcttctt
      721 cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt tctttttcgg
      781 aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata tgattttgca
      841 ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct acaccgaggt
      901 tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga gcctcaaaaa
      961 tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct aaataatgat
     1021 caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc aattcaaaag
     1081 cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag ccattttttg
     1141 tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag
     1201 ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt
     1261 cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag taaaaatggc
     1321 ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt tctttgacaa
     1381 cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca gcaaaatgct
     1441 cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta cgctgggcta
     1501 ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac atgtggatac
     1561 actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga cggggatttt
     1621 ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa cgatctcata
     1681 ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt tttcatcaag
     1741 cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt gtcggcgttc
     1801 ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat gttcaggatg
     1861 attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt ccaatacttc
     1921 agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa aaacttccca
     1981 taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag tcaattgtac
     2041 accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa tcatttcact
     2101 gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga ctttcgttgt
     2161 atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg tcatattcga
     2221 tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac gttaattgac
     2281 tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa aacgaaagat
     2341 gatagagaag actcattctt tcgtttataa aggatatata tatacattgg gagggatcta
     2401 tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg
     2461 ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat
     2521 ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat
     2581 ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag
     2641 tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt
     2701 aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa
     2761 aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat
     2821 ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat
     2881 agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg
     2941 accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc
     3001 atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg
     3061 tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg
     3121 gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag
     3181 acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa
     3241 cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg
     3301 ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca
     3361 cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg
     3421 cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa
     3481 tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg
     3541 cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg
     3601 tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag
     3661 gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa
     3721 aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc
     3781 attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata
     3841 tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa
     3901 ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg
     3961 atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata
     4021 tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg
     4081 gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag
     4141 aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc
     4201 ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa
     4261 agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg
     4321 tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact
     4381 caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg
     4441 cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag
     4501 cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg
     4561 aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg
     4621 gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt
     4681 atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat
     4741 tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac
     4801 aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga
     4861 agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt
     4921 tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa
     4981 cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg
     5041 aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt
     5101 atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct
     5161 tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct
     5221 tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga
     5281 cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga
     5341 ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact
     5401 gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct
     5461 ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat
     5521 ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc
     5581 ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag
     5641 aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag
     5701 agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg
     5761 aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa
     5821 gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag
     5881 aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc
     5941 gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat
     6001 gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca
     6061 gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt
     6121 gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg
     6181 ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt
     6241 gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg
     6301 attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag
     6361 tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat
     6421 gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact gctctttgga
     6481 cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta
     6541 atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa
     6601 gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt
     6661 gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt
     6721 ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca
     6781 cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt
     6841 ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg
     6901 gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta
     6961 cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa
     7021 gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac
     7081 agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat
     7141 ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa
     7201 accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc
     7261 cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa
     7321 atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat
     7381 aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga
     7441 gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa
     7501 ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct
     7561 tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct
     7621 gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc
     7681 atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca
     7741 ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa
     7801 gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt
     7861 gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg
     7921 attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa
     7981 aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa
     8041 actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc
     8101 aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg
     8161 cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag
     8221 atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc
     8281 tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc
     8341 ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat
     8401 gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc
     8461 gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg
     8521 atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt
     8581 ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa
     8641 attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa aataaaaaaa
     8701 tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta taaaaaagag
     8761 ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt cttgcgttat
     8821 ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt
     8881 ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga
     8941 aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata ataatttaga
     9001 atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga aagctcaatt
     9061 tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc aagactatgg
     9121 catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta
     9181 tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat catggggtta
     9241 atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc tgctaaaatc
     9301 ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact tatacaagcg
     9361 catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat gcaagtgaca
     9421 ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac tgatttagca
     9481 aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact tcttttttga
     9541 acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga actctcttta
     9601 tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg atggagaaat
     9661 acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg
     9721 cttttatcgg catactgccc gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa
     9781 ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta gcagcagaca
     9841 aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc tggaacggta
     9901 cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt gcccgagctt
     9961 atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt caaggtattt
    10021 cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat gtcgcagatg
    10081 caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca gttggattat
    10141 ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt atgatcatgg
    10201 gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg atcaaaatgc
    10261 ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca gcgacgatcg
    10321 ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat gggcaactga
    10381 tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct caagggttgg
    10441 gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt attcatggat
    10501 caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta atcaatattg
    10561 atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg
    10621 aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca tggtttggcg
    10681 gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa cgtgcagatt
    10741 atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac gaaccagtga
    10801 tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg attgctccag
    10861 tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct ccggtatcgc
    10921 agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca ggagcggatt
    10981 ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt tgggcaccat
    11041 ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa gatgaacgtg
    11101 aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg
    11161 attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga aggatacaat
    11221 gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca
    11281 agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa agcacagcct
    11341 tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta aaaagatagg
    11401 atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt gatcgtgttg
    11461 tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac aggtcttttt
    11521 ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga agcaaaggag
    11581 aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt ttttacgata
    11641 aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa
    11701 aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg gctcgcataa
    11761 aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca acgaagaatg
    11821 aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag gttcattaca
    11881 cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa
    11941 tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc gaagcaacgt
    12001 attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag ctagaaggaa
    12061 tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg taggaagggg
    12121 cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg aaatgtttga
    12181 tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac gttttcgtac
    12241 acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc taacaagtca
    12301 agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa tggccggcat
    12361 tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc agatcatgga
    12421 aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg cgccattctc
    12481 ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca gttatcaact
    12541 aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc gtatggattt
    12601 tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa accaacgagc
    12661 tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag gcaattacca
    12721 gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt atcagcttgc
    12781 aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt tttgcagcct
    12841 tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt catgcggcta
    12901 cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa tgatcacacc
    12961 ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc catttaaaaa
    13021 gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat ggaatgaagg
    13081 aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa
    13141 actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag ggtatcgtaa
    13201 agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg aagaaacgtt
    13261 aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat aaaaaacggg
    13321 tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga aggaaaatgt
    13381 taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt tttagggttt
    13441 tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt tttagatgaa
    13501 tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa ggacctccct
    13561 acattatttc attctacctc tagtttatca tctgttaagg actagtggaa acgttttgcc
    13621 taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa acaagctaca
    13681 attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc tcctaagcga
    13741 taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc ctttgcggta
    13801 caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat ttttatataa
    13861 actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc gaacaatatg
    13921 aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag
    13981 tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc catgcaaagt
    14041 aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc tgacggcgtt
    14101 tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt ttttgatttc
    14161 aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat caatcgtatg
    14221 tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc attcatttaa
    14281 tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga tatcttccca
    14341 tgttaatcct agaagttcag caaatcgcat tccagtggca ccagcaacta acacgagcat
    14401 aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag atttaaattg
    14461 atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt tgattactgc
    14521 ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca acgacgctct
    14581 aatatggtta ttaaacttaa taacggtaga ttttacatgg tctttagcat acttatttaa
    14641 aaagcgttga tatcccactc tatctaaatc agaaatcaat accgctggca tatatttctt
    14701 tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg ttccttcttt
    14761 ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc gatccccttt
    14821 aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt ctccttttgt
    14881 cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt atgaaatttc
    14941 gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcag ctctctttct
    15001 ctgatacaat aggaactata aagaagccca ttgtttgggt tttattattt tttgcacatc
    15061 ctttttgctt gccggcgcaa ggatgtgcta tttgtcgttt taatggtata attggatgtg
    15121 tattagatca tccaatcaga ggtgtttatt atgattaaag tgatttttaa aagaataact
    15181 gaacttccag agtttagatt atttgttgat tattggtatg tctggttttt tattgttgca
    15241 ttgctgatag gtctagatgt catattagaa agaaaactta agtaagagtt gagttaataa
    15301 attgaattcc taaatgagat attttgtagc ccatattttg tttgccgaca tcacctaata
    15361 ctatggagct aatcgttttt tctaacagac caaaatcaga taatctattt aagctaccat
    15421 ctattagctc ttgtgaatag ccagacatgc tgatatttcc agtgaaattt tcgtttccac
    15481 gactcattaa gcatgctaac aattgaatct caatcgatgt taattgattc agtacaagaa
    15541 gataatactc ctcgttatcc catgaagcgt tgttaattcg aagtaaacaa ttcttataaa
    15601 gatttttata catatctatt ttattttgtg cctttgattt ttctacctcg tcatttatcc
    15661 cttcgaatat tccaatgagt tggtccttgt tctctaagtt gaaaacatct tctggaattt
    15721 gatcgcgaat gttttctaat gattcgttta aatttttgta gaatttctcg atgcgtttga
    15781 aacgcttctc gtttttagac ccaaaataaa gtgactgcac aacgccacca atataaggaa
    15841 ttgctccaat accgccttca actattaatt caagatattc ttttttggtt agtttttcat
    15901 tgctattatc catagttacc tcctattttg tcatttcctt tacaagcatt gtgactcctg
    15961 atggcactct tttgtatagg ttttattatt gtgcacaatc taactcttag tggggcgtgg
    16021 ttcatttatt ttttacttac tttactatta atcatatccc attgagcata ggatttttct
    16081 atgttcgatc ctcttgaact aatttctcct gtaaaagtta ctttatcgcc ctttgtaaag
    16141 gtatcagcat tgtttaagtc cttcgcaaaa attacatatg aaactttagg tgttgcagaa
    16201 atatcattcc aacttttatc atcgtactct ttgtctgcta taatggctat tctagtagtc
    16261 attgattcga taacagttcc ttcaaaagta tatgttttac cactaatata tttagtaaaa
    16321 gctgtatttt gctcagtagc tggagtaata gaataataac tatctaagaa agcaccccag
    16381 tcatgattat ttgtctctaa aaattcttca aattgagtta ggcttttatt gctgctgtta
    16441 gatgtagtag tttgagtatc agatgatgag ctttcctgtt tgttattgtc atttgcctcg
    16501 tttgatgagg aaagcgagaa acctacggta gtaaaaataa tcccgataat aaggagaatc
    16561 ataggtgttt tcttatttgt ttttttgata aatcttacaa ttaacatgat taaacctata
    16621 ataaataaaa caataccagt aaaaccaaat ataccgtcca ttaatagatc ctctttctct
    16681 tttgatattg aatattataa accaagcaat tcttttttct taaaatcgaa ttcttcttga
    16741 ttaataatcc cttcatctaa caactcttta tactttttaa tttcatctgc acttgaaatt
    16801 tgtgtttctt tggaattatt atctaatata gattgttgct tctctatata tttttttaaa
    16861 ttttccattt cattccaata tttttttgta aacataacgg tgttttcatc tttcgtggcc
    16921 gctaatatac ctccgcgact ttcgtttccg cctagcaagg taaattgtat ataaccatta
    16981 ctcatacctg gtttttttaa ttgtacggct gatatatttt taaaggggat agttttttct
    17041 cctttaatac cttgattaac taaatttata aatccttttc taataattga aatagaatca
    17101 tcagttactt taatcaaggt ttttcctgga gattttatta aaatctcttt ttccattttt
    17161 tcttcctcat ttctgtgata tgattttttt tgtagaagct catatatgag gaaagagccc
    17221 gtgttgcagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa taattcttga
    17281 tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca ttggcagaca
    17341 gcataacaag tgtatctgag cttattctga atagaataat actccataaa tttttcgaga
    17401 ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa aattagaata
    17461 gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa
    17521 actgaaaatt caaaatgaga ggcgctgaaa tgtgcaagtt catgagataa atgaaaggct
    17581 tctgcagttt caccgtatag attttcattc aaaaaaatga ttctgggttt tggatagtag
    17641 aaaccaggct ctttcatctc catataaact actttcaaat tgtattcgct caacatttct
    17701 ttcaatttca aatacataca aaccatcact ccaactattc attttcctct aaagctttag
    17761 caattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca aaagaaaaaa
    17821 cagtatcgtc ttctgataaa tccacatgtt taggggtttc tctttcttct ctacctagaa
    17881 ggtagtctac agagacatcg aaatagtcag caatccgact taattcatct gaattaggag
    17941 tattattttt ccactttgct agataaccgt tagaatatcc aaaattaatt tctaactgcc
    18001 gtatagatat ctttttcctc ttcgccaagt cttttattat ttcataggta ttcatcgata
    18061 aaacaacctt tctaaatgtt tacaaaaaaa gtttagaaaa atacgcagaa ataatttgac
    18121 taattctgag taatgagcta tactatatct tgtaaacaga tttaacaact aaaaagacaa
    18181 caaaaaacac tattgatatg taaatgcaga ccgccaagaa agctttaaaa tcaatgtttt
    18241 tatgtcttat ttaattatgc tcttagtata gagtattact cagatactgt caattgaatt
    18301 tagaaaaaag ttgttaaatt tgtttacgaa tataaaagaa aggaatgaga aatatgaaca
    18361 caccgcaaat ttttaatttc gaacaaaacg aagttcggac aattttagta aatgatgaac
    18421 catattttgt aggcaaagac gttgcgagtg ttttgggtta ttcaaacact aaagatgctt
    18481 tgtcgcggca tgtagatttg gaagataaga tggggtcgcg aatcacgacc tcaggtcaat
    18541 caagagagat gacaatcatc aacgaatctg gtttgtacag tttaatctta aaatcaaaac
    18601 ttccctctgc caaaaaattt aaacgttggg taacaagtga agtgttgcca gcaattagaa
    18661 aacatggagg ttatctaact ccagaaaaag tagaagaagc tttgcttaat ccagatacaa
    18721 tcattcaatt agcaactcaa ctaaaagaag aaagaactgg aagattaatc gcagaacaaa
    18781 agattgcaga gtacgaacct aaaatctcct atttagatag catattatct tctacagatt
    18841 cagtaacaat tagtcagatt gcagcagatt atgggatgtc tccacaacag atgaataaat
    18901 tacttcataa actaggtatt cagaaaaaag tcggtaacca atggttattg tgcaaaaaac
    18961 acatgaacca aggatacaca aaatctcata caactgagat cccgaaagcc gatggtggca
    19021 ctaaaattgt aatgaatacc aaatggacac agaaagggcg tctatttatc tacgaattac
    19081 taaaaaaaga aggatattac cctcaaatgg atttagagga aattggttag aaaggagttt
    19141 tagtatgact gacattgcag aaatcactca acgagataga gaaaaaatca aagaatatgt
    19201 cgaaagttca aagttcttaa cttacaccat gcttgctgaa agatttggaa ttagcaaaag
    19261 ctacttatct ttaattttaa acggtaaaaa gacttctgca gaagcaaaca gaattataga
    19321 ttcgattatc actatgtacg aattgtaaag ggaggaacag ctaatgcaat atctagaagc
    19381 aaaaatccca attccagaag gctatgtaat tatctcccaa gtggattatg aggagttaaa
    19441 aaaagctgat gatactggta gatggatgac gttgccagaa gtgctagaac ggattaacag
    19501 aaaatatgat tggtttactt ctagagtttt aaagaaccca agatatagaa atattatcga
    19561 tatcgagaaa aacaaaaatg gatttgtcta ttatccagtt gaaggaagag acacatatct
    19621 atttttaaga agtaaaacac ttgaattttt agaaacaaat ttttcggaaa tcttaaggag
    19681 gcaagcggat gggaaaattt aatagagcgc tagtgttcag cgcaccgcta atcatctacg
    19741 ctttaggact ttggggaagc aggcaagcgt tgataggaac gattgtttac atggtttgga
    19801 tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca accgaggggg
    19861 ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac tcagtttaat
    19921 tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat tttgtggatt
    19981 tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt attaaaatta
    20041 attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg aatatttagc
    20101 ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc aattaatttc
    20161 ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc taaattaact
    20221 aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat catatctgca
    20281 gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt tatttttatc
    20341 cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa aattgttaag
    20401 tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa aaaggagaga
    20461 agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta cgagcttaca
    20521 agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg atgcactaaa
    20581 aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag attcatatgg
    20641 cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg ctatggggtt
    20701 tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca acgaaatgga
    20761 agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga tctatcaatt
    20821 agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta tgcgaattag
    20881 cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg gagttctagg
    20941 gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa aattttggtc
    21001 tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta agagattcga
    21061 tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta tggaaattga
    21121 tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct gaagcgctaa
    21181 gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc atcctaagcc
    21241 aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc cgccggcaag
    21301 caaagagtca caaagaaaac acatcataag gagattttag catatggaaa aagaactttc
    21361 cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg aggaaacaag
    21421 tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg atcaagatga
    21481 tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat cgaagcattc
    21541 gaataaacct gaatatattt acgtcatcga agatggcttg attgatgcgc tagaagagta
    21601 cgacagagat aacttgattg atatctctta ttacggacca ggtaagaaat acattgctga
    21661 aatggaggca gaatttgatg agtgaaatca aagggacaac gaactttgaa aaacttttta
    21721 gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta tcttgggctc
    21781 acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt aatgagtaca
    21841 aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa tataaacctt
    21901 ttcttatgga tgaaactggg acttatgtat ctgtctcagt aacggttaaa ggacacacgg
    21961 aaaccgaatt atttcctgtt ttagattatc gaaaccaacc agttgttaaa ccaaatgcaa
    22021 tgcaaatcaa taactcattg aagcgatgct ttgtgaaagc attggctcta cacggactgg
    22081 gattatatgt atttcaaggg gaagatattc caacaccacc tagaatcgat acaaagaaat
    22141 taaacatgct agagacgatt ctagaagctt tcaatgagca gatgggtaaa gatatgacca
    22201 aaaccttaat cgaatatgtt aatgagcaga cagataaatt agggctctta gctgataacg
    22261 ttgaaactat tgaacagtta agctatgagc aatgtgcctt gatggagcga gcaatagcag
    22321 ctaagagaaa agaattagat aagaagtgat atgagtgttt aaaccattaa tcgattcata
    22381 ttcagcggtt ctgaaaaagt tcaaaggaaa agacataagc gcaaccatca atgaggaagt
    22441 gaacattgat cgactaaaga cgatgtatga cggctacgat ggtgatcgag tcattgaaat
    22501 tcgttttatt gatcctagac gtttcaccgt acagcaacga aacttcatct atgcgctgat
    22561 aggcgatatt tttatcgata caggcatgcc aacggacttc tggaaggaat tcttctactt
    22621 tcgttttgaa ggtgtcacag ggcgcaaaat aagcctgaaa gacgaatcga atacaactgt
    22681 gagtgatgcc aatgtcttag caaatatcat cttagatttc atctttgaac atcatattcc
    22741 tttcaaagaa ggctatgaga ttttacctgc gaatcaagaa tattacttct acaaatgcat
    22801 cacaaaaaga gtctgctgca tctgtggcaa aacaggagct gacatcgatc actttgacaa
    22861 agcgctagga agacgaaagc gcaaagaagt tgatcatgca gagtacacat ttgcagcact
    22921 ctgcagaatc catcacacag agaagcacaa aataggtgtg attaatttca aaaataaata
    22981 tcaaatcaaa gggatcaaat taaaccagaa aacaatcaaa aagttaaata ttggagggga
    23041 aaaatgacag aacatcgaag ttattatgcg attataccag ccaacgtaag gtacgacaaa
    23101 agacttaaac caaatactaa gttgttatac ggagagataa cggccttgtg taatgaaaga
    23161 ggcttttgtt gggcaggcaa tgagtacttt gcagatttat atggtgtgaa taaagagacc
    23221 atatcgcgat gggtaagtga tttgattaag tttggatact tgaatcggga aatcatttac
    23281 aaagagggta ccaatcaaat aatcaatagg tacctacgaa ttaatcaata ccctattgac
    23341 gaaaaacgca atacccctat tgacgaaaaa gtcaaagata ataatacatc tattaataat
    23401 acatttaata atacaaaaga atatataaga gagttaccgc cttcgaaaaa atcgaaggct
    23461 aagcccgtcc gtcataaata cggagagtat aaaaatgttc ttttgtcaga tgagcagatg
    23521 gagaaactca aaacagaatt ccctaatgat taccaagagc gaatcgaacg actgtcagag
    23581 tattgtgaat catctggtaa gacttataaa aactatttgg caactattcg aagttgggca
    23641 aggaaagaaa aaagtgaacc taagaacgca agcagtggat acaagcgcac aggaagacga
    23701 gagaagcttc ctgaatgggc aatcgaccaa gaagcctatc tcaagaaaaa agcgctagaa
    23761 cgagctaata gacaatcaaa agcaccattc taagaggtgg aaaaatgaag atcgattatc
    23821 tagaactaat taatgaaata gcgaattata aaaagggcga ggaattagac gtcctgagag
    23881 acgtatatga tcaactcgaa gaagctggaa ttgaaggaat taagaatgat cgttcgagtt
    23941 ggagtaaact cagatactat ttcgcactct atatcgatgg aacgcaatta agaaatttag
    24001 catataccaa attactattc attgattgtg ttaaaggctt gcaaaaacat cttaatgaac
    24061 ttgagcaggt gtaaacaaga tggacctaaa gacatttaca gcacagatcg aattaatgca
    24121 tcaagaagct ttaagacaaa gtgtgtcgta cgaagacaag tggctcaaca catttcatgg
    24181 cggacgtgag agcgcacttg atcaagtact caaattactg aaaggagaat gtcgggatgg
    24241 ataagaaagc ggcaatgcag cgaattatcg aattgactta ttcagaagat tggcaggaag
    24301 ataaaaaaat agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca
    24361 aacggagaac gccgagaaag attgcaatct ggcatggtga tcgaattcta gtaacgggta
    24421 ccgctaaaca gttatctgaa attactggtc tgagcaaaaa tattatttgg gatagagcta
    24481 ggagcttatg gattgattca aaaggaagac agtttaggta tgtggaggag aaataatgga
    24541 cgaactaatc gcgaaagtag agcagtgggc aaaagataag ggattggatc aagcggattt
    24601 cagcaagcaa atgttaaaaa cggtcgaaga aatcggggaa gttgccgctt ctctagctag
    24661 aaaagatgaa catggtttaa gagacggaat tggcgatgta gtagtaacct tgatcatctt
    24721 ggcaatgcaa aatgatatgg atttatacga gtgcttggat tttgcttatg acgaaatcaa
    24781 aggtcggact ggaaagatgg tcaatggtgt tttcgttaag tccagtgacc tgaaataatt
    24841 ccattaatgg aagaaataac tgaataggag gatagacatg attgatgcaa aacaagtgca
    24901 aaaacagaaa gatggaatgt tgatgtttga agcatatgtt ctaccatttc ttaatcaatt
    24961 tgaagttttg gaatgctctg ccagcggaga agaattggag tatgttgtaa ttcgtgaaac
    25021 caaagaaaac gttcagaagc taaacgaatt tctttgcact attaactgtt gggatatgat
    25081 agctccagga tttttatgtc cagctatggg agaatttttg gaatattgtc gtcttgaaga
    25141 tgcaggcact ttggatttag cttatctggt atacaactac ctaaacatca acacagatca
    25201 tttgtggttt ggcactgccg aaagaaaatg ggtagtccgc taaccaccaa tatcaatgaa
    25261 aaaaggcggt ttcctgaaag tggaattgcc gccattgctg taaggttctt tacgcgactc
    25321 aaggtcacag aaatataaat gcaaatctct tttgaagtga tccaaagctc tcggaaaatg
    25381 gaagtttttt gtttcaaaga ataaatgaga tttgcgtcaa gaacctgaga gttatttcca
    25441 aaaaagcttc caaagaaccg aatgagctaa ggacatcatg tgtaaacaca taatatagct
    25501 ctcctttcac ggctaatcat aacatgaaaa atattcgatg tcagtaggaa aaaaggtgaa
    25561 ttatgaaaag aatattaaat tatcccggca gtaacagatt ctgcaattaa actccgaagc
    25621 tgatgtatca gtatcaactg tagaatatta tgagcgtgga gaaagtcaag cagtcacaaa
    25681 tgtaaaaatc gataacgtca acggtcttat ctgtttagat gaaggcgata gtatgaatga
    25741 aattgctgca gaccatgaat tatcagaaac tgctttaatt gatcaaatcg aattagctag
    25801 acaatacttt gttccatatg cagatacctg gtccaagcat aagaatgaca tcatttttcc
    25861 agaggaacaa tgagagcaac tgatccagta attatccttg aggaagccaa atttatttgg
    25921 actcacgaag agatagagca agcacgcttg ctcttttctc aaggagttaa gccgagcaaa
    25981 gtagctgaaa taatgggtca aaagattctt gatgtcggat tgcttttgct ccatctagca
    26041 gaaaaaattt gatttgaggt gaaaatgatg attcaacttg caggcatcca aacagggaaa
    26101 atttattttt ctggagaaag caaaagcgaa gccaaccaat ggctattgaa aacttacacg
    26161 aacaataaga agctaaggaa aaaatatcct gatttgttgc taaaggatga tcagattatg
    26221 ccggaaccga tgattttgac ccgtaaggag ggaagttgaa actaaaaaac cagccgtgtg
    26281 ggctggagtt gctatttctt tttaataatg agtttttcta aatttaattc attctcacga
    26341 gtttgcgtcc aataatca
//
LOCUS       DAYYHS010000036        26232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000036 DAYYHS010000000
VERSION     DAYYHS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.036"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1006
                     /locus_tag="ACHA6A_001457"
     CDS             95..1006
                     /locus_tag="ACHA6A_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969662.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            2002..2253
                     /locus_tag="ACHA6A_001458"
     CDS             2002..2253
                     /locus_tag="ACHA6A_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969663.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            2366..2608
                     /locus_tag="ACHA6A_001459"
                     /pseudo
     CDS             2366..2608
                     /locus_tag="ACHA6A_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2698..2937
                     /locus_tag="ACHA6A_001460"
     CDS             2698..2937
                     /locus_tag="ACHA6A_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969664.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            3084..7367
                     /locus_tag="ACHA6A_001461"
     CDS             3084..7367
                     /locus_tag="ACHA6A_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8969665.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            7466..7831
                     /locus_tag="ACHA6A_001462"
     CDS             7466..7831
                     /locus_tag="ACHA6A_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969666.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            7833..8285
                     /locus_tag="ACHA6A_001463"
     CDS             7833..8285
                     /locus_tag="ACHA6A_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969667.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            8734..10209
                     /locus_tag="ACHA6A_001464"
     CDS             8734..10209
                     /locus_tag="ACHA6A_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8969668.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            10232..10543
                     /locus_tag="ACHA6A_001465"
     CDS             10232..10543
                     /locus_tag="ACHA6A_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969669.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            10903..12159
                     /locus_tag="ACHA6A_001466"
     CDS             10903..12159
                     /locus_tag="ACHA6A_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8969670.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            12156..12701
                     /locus_tag="ACHA6A_001467"
     CDS             12156..12701
                     /locus_tag="ACHA6A_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969671.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            12702..13202
                     /locus_tag="ACHA6A_001468"
     CDS             12702..13202
                     /locus_tag="ACHA6A_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969672.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            13561..13839
                     /locus_tag="ACHA6A_001469"
     CDS             13561..13839
                     /locus_tag="ACHA6A_001469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969673.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            13859..14203
                     /locus_tag="ACHA6A_001470"
     CDS             13859..14203
                     /locus_tag="ACHA6A_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969674.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            14222..15133
                     /locus_tag="ACHA6A_001471"
     CDS             14222..15133
                     /locus_tag="ACHA6A_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8969675.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            15212..16204
                     /locus_tag="ACHA6A_001472"
     CDS             15212..16204
                     /locus_tag="ACHA6A_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8969676.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            16201..16419
                     /locus_tag="ACHA6A_001473"
     CDS             16201..16419
                     /locus_tag="ACHA6A_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8969677.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            16432..16842
                     /locus_tag="ACHA6A_001474"
     CDS             16432..16842
                     /locus_tag="ACHA6A_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8969678.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            16894..19422
                     /locus_tag="ACHA6A_001475"
     CDS             16894..19422
                     /locus_tag="ACHA6A_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8969679.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            19438..21648
                     /locus_tag="ACHA6A_001476"
     CDS             19438..21648
                     /locus_tag="ACHA6A_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8969680.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            21656..22747
                     /locus_tag="ACHA6A_001477"
     CDS             21656..22747
                     /locus_tag="ACHA6A_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8969681.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNFTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            22768..23310
                     /locus_tag="ACHA6A_001478"
     CDS             22768..23310
                     /locus_tag="ACHA6A_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8969682.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            23505..23762
                     /locus_tag="ACHA6A_001479"
     CDS             23505..23762
                     /locus_tag="ACHA6A_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969683.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            23776..24108
                     /locus_tag="ACHA6A_001480"
     CDS             23776..24108
                     /locus_tag="ACHA6A_001480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969684.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            24153..24701
                     /locus_tag="ACHA6A_001481"
     CDS             24153..24701
                     /locus_tag="ACHA6A_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8969685.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            24708..25160
                     /locus_tag="ACHA6A_001482"
     CDS             24708..25160
                     /locus_tag="ACHA6A_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969686.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            25257..25598
                     /locus_tag="ACHA6A_001483"
     CDS             25257..25598
                     /locus_tag="ACHA6A_001483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969687.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            25966..26157
                     /locus_tag="ACHA6A_001484"
                     /pseudo
     CDS             25966..26157
                     /locus_tag="ACHA6A_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         9087 a         3740 c         5579 g         7826 t
ORIGIN      
        1 ggcgatacta aaattaacaa aattggaata tttttcttga agatttttag ctgtcagtat
       61 atgatgaaag catctcatag gaggtgcttt tttcatggtt gataattcaa gtggaagggt
      121 tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa aagttagctg atttagcagg
      181 tatttcacaa agtactttag ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt
      241 agataatact ctaagcaaga ttctgaatat agaaactcta ataaaagatg aggaagataa
      301 gattgagatt ctcatcggta aactaattgc atacagagat atgaataagt tacttaacaa
      361 agagttagct gataatatag gaattagtga agtattactc agttatgtgt taaatagaaa
      421 gagaaatccg tcaaaagaaa tgcaaaaaaa aatcgatatt tttctattaa gtaatgaaaa
      481 agaaattttg aaggagatta atcgtgatag tgagatattt agtttatcaa aagacgataa
      541 aattgttatg ggaaaaagaa tacgagaagt ccgaaagaac cgggaagaga cgcttgaaaa
      601 atttggtaaa aactttacca tctatacagg caaaaatgtt ataagtagat gggaaaaagg
      661 tattaatatt ccagatattg aaaaactaat gaatattgcc tatcttggta aggtcactgt
      721 tccttacctt atgtacggag aagattataa gaatatactt ccaaaagatg aaagagttag
      781 cgattttaaa aagataaatt cttttagtat gggattaagg atgaggaaaa ttaggaaaga
      841 ttattatcta gaaagggagg agtttggaaa gcttttttct ccttcaattt caaaatggtc
      901 catagatagg tatgagaatg gtagagatat acccaatact aatcgaatta tccaatatgc
      961 ttatatagga aatctttctt tagaattttt gatttacggg atataaaaat agtttacgtc
     1021 gatacttatt aagttatcga cgttttttat tatagaaaaa atttataaaa ggaggtttct
     1081 tgatatgata agaaacaaaa agggtttgtt gaaagttgct ttactggcag cttttttcta
     1141 agtaactttt tgacaagctc aatggcaaca caagcggaag aagtaataca agaactaact
     1201 gttatgaata acagctagat aaaatgggct atgtatgtta tccaacaatt tagtaatcac
     1261 tagaagccta gcgaaagttc gcttgataag ttatagatga gtattgattt gtagtgtttc
     1321 caaatcaaaa gggtacagag ccacatgaaa tatgttgtcc tctgcagcgt caggatggga
     1381 atccatacca tggagattcg gagatctgtg agggaatact tacgtggtta atatgtcagt
     1441 tgtgttggac gtagtaatgg tcatagtggg tagattgact gaattcttat atgagaaaat
     1501 gaaaccaaca agaagatagg gttatttata cagaattggg gatcttttct ggaaaagtaa
     1561 tcggataatg cgagattcgt acggtctttt ttaaatttct ctaaaaaaaa cggcatcgaa
     1621 tgggttgggt gattgataag caatgaatca tagactgata tagaataaaa aatgattcta
     1681 tttatctttt tacatctatc gttttttcaa gtagccgcaa attatatcag cattcaacaa
     1741 tttttgtata caagttctag taaaaggatc tgaagaaaaa gttggttgtt gtgaagataa
     1801 aaaaagaatc aggttttact gagtttttct tcaaaatttg tgttattagg ttaggacaaa
     1861 gataagaaga agtatgaaga tttaaaaaag gaatgaggaa aagatagtaa tttccgatta
     1921 ttggagtttt tttatttata aagaagaagc gaataagttg tgattcctag atttcgcgcg
     1981 tgattaatta atgagcaaaa aatgcggaaa gttttagtgt ttaatacacg aggacaaagt
     2041 ttcttaggta ttgaaggcac acgtgtaacg gttgatgggt ggtgtgattg tttcgaggtt
     2101 ggaaaacatg ttgaacttat ggcatttact gagctaaaag ataagaacaa cgtggaattt
     2161 ttcgagagtg atattggttg ggacgatcat gaagaagttt atggaaaatt tattttgcgg
     2221 aagggaaatt cttatacgaa tggggaagca taagtagcga tttgttagag atcattgata
     2281 ggattgaaat tgtcgggaat atttatgaga atctagagtt atttgaggtg atagaatgag
     2341 tgatggtttt gaacaagtat caataattaa aaatcaagtt cgggagattc ttagaaaaaa
     2401 aggctatttg gtagatagtt attttgaagg agattacgaa acatgggtag gtgtctatgc
     2461 tcgtccagag aataaaccta cctatttaga tcctacgacg tcagaagatg cctatttaca
     2521 aaataggtat cgagtagatg gttttaagca agactttgcg gagtggtttg aatgggaaat
     2581 tgaaaacgga gaagtaaaag aagaataaga gtacttgttg tatcaaaata agccagtaga
     2641 acaaccaaac cagtagctag taatgacaat aatactgagg aatcggatca ttatggtatg
     2701 attgatattt ctcttccaga agatcttaag ccagatcctg taacaattgg caatagtgtc
     2761 atgatttttg attcattaga tgaagctatg tcatatggtt ggaaagaaat taaggatgaa
     2821 ggtagtaatt ggtatggcat gagatttggt gatggtgaag ttgccaaaaa agctggtatt
     2881 ccaattggga aatggacagt tgatttccac gaagcaggtt ctttttttgt tctgtaatta
     2941 tagtgggagg gtaaaatcat tgatgaaatg tgttcagata agagcgccag ataaaaatag
     3001 aagagacagt tcacgtcgtt atcacttttg gtaacggcgt tttgttttat tatataaaat
     3061 tctcaggaaa aggaggaatt cttgtgtatg caaaaacgaa aatgaattgg ttgaaagcac
     3121 tcgcattggt ggctttttct caattctgtt tcttggtggg aactgctcaa gcacatgcag
     3181 aagaaacgac ggctatacaa cctacgatgg aagccattgc ccaagtcgct tctgaagtga
     3241 cagaagtcca accagcagaa gccggggtcc cagctggtga agccactgtt gaacaggccc
     3301 ctgtgacaga agcgccaacg gtcaattcca aaacgactct cgatcagacg attggggaaa
     3361 cacaacaccc agggaatagt cctggtactt cacaaccagt tgaagaaact gcctcaatta
     3421 caacccctgc ttcagaaacg tccactctag aaggagaaaa agctgccgaa gcaacgcctt
     3481 ctgtgcagcc agagtcttct gagaaacaag cggaagaaaa gaaacgaata gaagaaacag
     3541 tggaacaacc agcggctact gctgacaaag aaggaaaaaa tgaggaaaac gttgaaaaaa
     3601 caatctcttc aaccgcagct agtcaaaatc aaccaacgaa accaaacaaa gaatctacgg
     3661 ctagtattga aaatcaaaag aaaaaagagc agaacattcc aaaaacaggt acacctacac
     3721 tgttagtaac agtaccaacc aatcgttcgg ttcgtgttcg ccgtgcaacc aatgcagctg
     3781 ttttaggtgg tcggaaaatt gattggggcg aaaatattgg tgttacttct agtccgaaca
     3841 atctaaactt cactttaaat tatatggttc gttttgagat taatggacaa gtggcgtttt
     3901 gtattgagcc tggtgtctca gccgcagaag gtcaagttta tacagctact gctttgaaaa
     3961 actatttgaa aaattcaaat gtacggaaaa aaacaagctt gatctcttat ttaggatatg
     4021 tcacaaataa agacaaatcc gatgaacaat acattgcggc acaatttatg atttgggaag
     4081 ctttgggcac aaaaatccca atcaaaaata tccgtgttga ttatgatcgt cgaaaagcag
     4141 aaattcaacg tttgattaat aactttggtg aaaaagcaac ttttaataat caaacacata
     4201 cggtaaaaat tggcgaaacg ttgaaagtaa agaataaaaa tgattttttg aaatatgtga
     4261 aagacattca aaaaccaaaa ggtgtcaatg tatctattca cggcaatgaa gtatggatta
     4321 cagtcacaaa agaggcaccg gataaagcaa aaattcaatt gaataaaatt aaagttcaag
     4381 gtgcgccatt agcctatcgt cgtccaggag ctcaaacaat tggtgtgtta catccaacag
     4441 aacctaatgc gtctgttatt aatttacaaa ttctaaaaca aggaaacatt cgtgtgttta
     4501 aagaagatgc ggaaactggt gtgaaagcac aaggtgcagc ttctttagaa aatgcggtct
     4561 atggtttgta tcaagcagat gggaaaaaga ttaaagaaat taccttgaaa aaaattaacg
     4621 gcaaagtaca ggcagaaatt aaagatcttg atcctggcaa atatgtttta aaagaaatca
     4681 aagcgccaaa aggttatgtg ctttctgatg aagcaattga aattgttgtt gaacctggaa
     4741 aaactgtcaa tgcgaccgca aaagatcaag tgatcaaagg aaccattgat ttagtgaaag
     4801 ttgctaataa agatttagtc gattcaacga atccagataa caaaccaaaa ttagctggca
     4861 ttgaaatcac tttgacgtca aaaacaactg gcaaagtggt gaaaaccgtt gtgacagaca
     4921 atgatggtta tgctactttt ggtaaaaata ccgttgtctt tgatacgtac attctttctg
     4981 aaaccaaagg aaaagaaggg tataaactat ttgaaccact tgaagtcaca atttctgaaa
     5041 atgggcaaac tttccattat gttttagaag ataaagtaat tgaacaacgt cttaaagtgg
     5101 tgaaagtgga tcaagaaaca ggaaaagtga ttccacttgc taatacagaa ttcaagattt
     5161 ttgatacttt agccaatcgt tatgtcacaa tggagattcc aaatgataca gaaacaacgg
     5221 atatctttaa aaccaatgaa aaaggctttt tcctaactaa cggtacactt acttatggaa
     5281 aagatcgcta tcgtttggaa gaaatcaaag cccccgtaaa ttacgttttg aatcaaaacc
     5341 cattagtgtt ctcgattacc aatgatacag aaccgatcaa agtgatccat tttgcgaacc
     5401 gtcaagcacg gaaaagtgta aaattattca agtatgaaga atggaacaag aagaaaacgc
     5461 cgctcgcagg tgtgaacttt accttgtata acggtaaaaa tcaagcaatt ggtgaatata
     5521 ccactgatca aaacggggaa attttagtga aaggtctagt tgccgatgac tattacttct
     5581 tggaaaatgc cccattagaa agtttccaac caaatacaga taaacaagca ttcacggttg
     5641 cattcaaaca aaatgcgcca gatgatgggg aaacgttagt ggtttctgta cgtaattact
     5701 tgattccacc aaaactagaa acacaagcgt caaataaagc cgatggtgaa aaagtggttg
     5761 atccattaga aacgatcgtc attaaagaca gtgtttctta taccaacttg tttgtcggaa
     5821 aagaatacac catttcagga gtattgatga acaaggcaac gaatgcgcca attttacaag
     5881 atggcaaaga aatcacaagt accttggcct ttattgcaga caaagtcaat ggcacaaaag
     5941 accttgaatt tgtggttaat gctagtgttc tgaaaggtca aacgatcgtt gtttttgaaa
     6001 aacttttccg tgatggcatt gaagttgcag cacatgcgga tatcaacgat gaagcccaaa
     6061 gtgtgaaagt gaataatcca accgttcata ccacggctgt ctttgaagac ggattgacag
     6121 tcgcagaccc aacaggattg tttaccttaa acgataccgt tgaaattacc gagattcttc
     6181 caggaaaagc atacttggtc aaagggatct taatggataa agcaaccggt gaacccttgc
     6241 ttgtgcatgg aaatacggta gaaacgagtg ttggctttat tgccaaagca gaaaatgaaa
     6301 cggtgatcgt accatttacc tttgatttga caggtataaa tggtcgtgaa atcgtcgtgt
     6361 ttgaaagtct ttttagaaat gatgaagaac tagcaaacca taaagacatc aacgacaaag
     6421 ggcaaacggt acgagtaact aacccaagtg tacgtacaac ggctacacat aaacccactg
     6481 gcttgaaaga ggttaatcca ttaacgaaag tcaccattac agacgctgtg aaataccacg
     6541 atttggtcgt tggaaaaaca tacacagtca acggtgttct aatggacaaa gtaacggaaa
     6601 aaccagtgct agtagatgga aaagaagtca caagtacctt aacgtttgtg gcagaaacaa
     6661 cggatggtac agtcaatcta gactttattc tagacgcacg tgcgttgcga ggcaaagaag
     6721 tcgtggtctt tgaagatctg tttagagaag gcgtattatt ggcttctcac gcagatatta
     6781 ccgacaatga ccaaaccatc aaaatcagta atccaaccat tacgacgaaa gcgacgaata
     6841 aaaatggcgg aaaagagatc ttggccttgc caaatcaaac tgtgcttgaa tgggtcaaag
     6901 tagacggtct tgcgattggt caaatctatc gcttaattgt tcaaggctac ttaacaccag
     6961 acggcacacc atttgaaggc actcatgcgg aaaaagtctt tgcggccgat aaagaaatga
     7021 tggaattctt atttgaattc ttagtggacg gtcgcaatct tgcaggaaaa ggcttgagtt
     7081 ttgcggaatg gctccaagcg aaaacggaag atgagaaaga aacgtttgaa gaagtggcga
     7141 aacacaatgt agatctaaca aacaaagatc aaacggttca aattgtggaa gcgccaaaac
     7201 caccgcaacc agtgaaacca gaacaacggg ttaaaccagc tactgttgta ccaatgttgc
     7261 caaaaacagg tgaacaaaca agtagtatac ttatggttct tggattagta gtattagtca
     7321 ttgctttagg tataacactt tataaagtac gtaaagccaa agattagtca tgaggaacaa
     7381 gcggttagga tttttaaccg cttgtttgat tgtaatgtag aaatatttag aatttatttt
     7441 ggtttttaag aggaggaaaa actttatggc agaaaaagca ttgcgtatga agaaagagtt
     7501 tttaggtcaa gtatttttat tgggttcacg ggaacctaat ccaccaaagg aaattaatgg
     7561 tgtgtctggg cgatatgttc aattacaagc agagtgttcg cgagaagaac agcctgcttt
     7621 ttttgtttat ctcacagctg aagaggataa aagattacga ttaccagaaa aagaggtgcg
     7681 tatttcattt gaagaagctt attttgtttt tcgtaaagaa cgagtgaata gtcgtgaaac
     7741 aaatgatatg ggacaaacag agcgtcgaac aactggctat caagatgttg gtgtcttttt
     7801 aactggtgta aaaattgggg aggttttgta agatgggatt aaaatttcca aataattatg
     7861 acattacaga agcattagat gaaacttgtt taggagccgt acgctttggc gcattagaag
     7921 agtctgatcg tgtatttgat aatgaaacag gcgattatac gggagaaatt cgatcacagc
     7981 gagcacaatt agtttttgga aatccagatg aaccaaagat tgcacgacga gttaaatttc
     8041 cagcagatgt agatttgact ggttttaaac gacgtgatcc tgtgaaatta attgatccag
     8101 aatatcatgt ggtatatatt ccagcacaag atggcaatcg tgaatattca gatttagtga
     8161 tttatgcgaa aggattagag aaagtagaag ctccaacaaa taatcctaat atgaaacaat
     8221 cacaagaaaa aggtttagat caaaaacaac caagtgttga ttctaaggaa aaagagaaaa
     8281 aataaggttt ttttgaaaga ataggaagga gtcttcctat tctttttatc taagatagaa
     8341 ggaaggggag attttgaatt cagataaaac caatcaaata ctttgagaga ttgtgcttgt
     8401 tatcaaaata tccaacaatt gattgagtat tttggagaca atccaacgat ttttgtacaa
     8461 acaaatgcat atatttgtgc ggttattggg cagtgtatgt ttttacgtga tcttgttcgt
     8521 ttttcattac cgtaccagtg gaaacaagat catcaggaag tgatttggat tcgtatagta
     8581 cgaatatgta atatgtgtgc acacccttat aaaaatcgat taaatctttt tcgattatgt
     8641 ttttttacta aatgtgatat tcctgtatta gttgcttttt gtcaaaagtt tttagcacag
     8701 gaaaagtaaa aacagaaagg aggaatgtga caattgaaag ctcatttttt caagtatcgt
     8761 ggtcggcgaa ttcgctattt caatcgttat ttatggtttt gctattatgg tttctttttg
     8821 tttccatttt tgattctgtt aggttggttt agttggaaag tattttatcc acgtttaagt
     8881 acgttttcga attggcaaat gtatcttatt cctggaataa gtattgtgtt gtatttgttg
     8941 ctggttagtg ggttaatttt agggttgatg cattggtcgc gactaaaaga aggatatttt
     9001 gcctctgttt attggcgtca gttacttgtt cgaatgttaa ttgacaatcg gtttgtttat
     9061 acaaagaaac aagttagtga gaaacaaaca agggaaaaaa tgagattacc aaaagtttat
     9121 ttttatcaaa agaaacaaga attagtggtt tcttttcctt tagatggtgg gcgttttcac
     9181 gaacgatttt taaaaatggg tcatttgtta tctgaagtat ttttgcgtga tttaattcgt
     9241 gaagaacgag aaaaagcccg aatcaattat gtattccttt cagattctgg tcggattcct
     9301 attgatcaat gtatagcaga aaatggacgt attcatttaa tgaaaacttt ggattgggta
     9361 tatgatcaat caccaaatat gttgattgct ggtgcaatcg gtggtgggaa aacctattta
     9421 ttgtattctt taattcaagc ttgtttaagt gttgggacag tagatatttg cgatggaaaa
     9481 gcggcagatt tagcagcatt aggtgacgtc gatgtattca aaggacatgt gttttacaaa
     9541 ataaatgaag aaatgattat ttgtttacga aatgcattaa aagaaatgaa tcaacggtat
     9601 gcctatatga aaaccatgaa agatcctaaa tatgaaccag ggaaaaatta tgcttattat
     9661 gggttaccac cgcattttat cttttttgat gaatgggcag ctttttatgg ttcgttggat
     9721 atggaagcga gaaatagagt cgataaattg attcagccat tagtattaaa gggtcgtcaa
     9781 gcagggatgt acattatttt agcaatgcaa cgaccagatg cagaatattt tccaagtggt
     9841 gttcgggata atttaacatt ccgtatttct gttggtcgat tgtctcccat tggttattta
     9901 atgacttttg gagaagattt taaaaataag ccattttaca acaaagatca acctggtcgt
     9961 ggatatgctt ttccaggatt tgatgtacct gtagagtttt ttgcgccatt tgtttacaaa
    10021 gattatcact ttatcaaaga atttgagaaa tttactgaaa tgattgtcta tgattttaca
    10081 gtaaaagagg atagtcaaga tcaaacgatg ggagatgata atcagttgtt acaagaacaa
    10141 ctagagcgcc aaatgtattt agcaaaagat ccaaaaggaa caagtttttc tgatgcgcat
    10201 attttgtaat caactaagga ggtgaagtta aatggcagag agttgttatt atagagtagc
    10261 tacagcaata cgaatgatta atccttctct ttcttccaga acgttttatg attggttgaa
    10321 tcgtattgaa caagtaactg attaccgctt tttgcgcaaa gaacgtgttt ttacaggaaa
    10381 agttattaat caagtattgc taacgaaaaa agatatagaa cgattgactc gattgtatca
    10441 ttatcgagta gatttagaag aggatttaac tttgtcaatt tatcgcgttt tttctcctga
    10501 aaaatatagt gaaataacaa aattagatca tttaattttg tagttattat ctatcgggag
    10561 ggctctagac gtagtctggt agccgcactc tgcctggcgg tgcgcattaa ggctgcgaag
    10621 catgggccta taatgcgcac tcgctagcag cgtgtgcagc tcacaaagag gtcgtgcgct
    10681 tgcgcacggc ctctttgtgt aaagcccccc gatatataat atggggggca gaaataacat
    10741 aacacataga aaaaagtttt ataaaccctt ttgtatcaag tgattatcta aggtcagcag
    10801 ttgtactact tttcccaaaa aacgaggggg actagttgat atatcggtgt ttctaaaggg
    10861 ttaaaaaaat cgattttgtt attttataaa aggagcgaga aaatgacacc gattgagcta
    10921 aaaagctaca gaaagaagtt aagtgcaatt cataatataa gtggagataa aaagccgcta
    10981 tcacaagcaa gatttgcgca attgatgggc tgctcaaaag attacattag aaagatggaa
    11041 caagggaaaa gaaaaatccc agaggatttt gaacaacgaa tcaaggaacg ctttcgattg
    11101 tatatgaaaa gcggaataga tcttcgagca atgattgatt atttgcgtct ttcctttttt
    11161 gggacagact acaaaacagt gattcaacgt gtattacgaa tggatctgac agaatttgaa
    11221 acacgtccaa cgggcttgta tctttttgac tgtctttctg ttcgtgggaa tatctgggtg
    11281 ttctggcatt caaaacgaac tgtaacagaa aatgtacttg tgcaattttc aggacaaggt
    11341 tgccgggaat atgaggaagt catgcgtgaa caacaaacag attggcgaac atggctatat
    11401 tccatttgga aaaatcaagg aaagttggaa gctggttata cacgtgtgca atgtagccga
    11461 ttcgatattg cgttagatga actttgggga ttggaaacgg aagaacattt cgatctcttt
    11521 gaattactag aaaaacaaaa agcaggttta ttagaaatgg actttaagag attccagaat
    11581 attggcggtt gttttttaga tgatggctat tttcgaagtg aaggattatc tttgtatttt
    11641 ggatcgcgaa agagtcctct cttttttaat ttttatgaga aacgttttga aattgcgaac
    11701 cgagaaaaaa tttcagaatt agaagcatta acgaaatatg gtatttataa tcgatatgag
    11761 ttacggttag ccaatgagaa agcaacacaa gcagtagaag catttatttt aggtgattca
    11821 ccgaaacgtt taggagagat tggcgttggt ttaattaatg caaaacttat tgtttgggat
    11881 agactggaaa gtgatgcacg gattgttgat aaacgatggg caaaattatt tcgttcgatg
    11941 gaagaattgg attttacagt aaagccagaa aaattctcat tagatcgttc agaacgctgg
    12001 tttttgaaac aagtagctcc gacattaaag ttgttacaag aagcagatcg tattttagga
    12061 agagactttg tacaagaagg gattgataat gccgaactaa atgacgagca acaaaactat
    12121 ctagaatgga tgggaacgtc aggagggaaa attgcatgaa tgcatatacg acaacagaat
    12181 ggctgcgcct gttggattta aaggctgcaa tcgaagcatt aaatgaaaag atggttgatt
    12241 tgtcttattt tcgttttcga gttccttata ttgaacaagc ggtaaaagct ggacgttatc
    12301 aggaaaaaga gaactggcaa gaaattgcac gcttattaga agttcgtaag ggatacgaac
    12361 aagaactcga agagttggaa tttagccgaa ggaaaggaag gctagaattc attcgctttt
    12421 atcgattttc attgccaata cctgcgattt tagcggttaa aaaaggctgc gataaaatga
    12481 agatatatga aaactgtgtg gcagcactta gcagcgaaaa acctcttatg gaagaaattt
    12541 cattggtgac tgtcacttgg gaaatgagtc gccagccaac agaagaacag acatatcttt
    12601 ctttagaaga gattgagata gaattagagg aaattggtcg ctatgccact tgttctacct
    12661 attgtggatc ggtcattagt attgctggtg tgattgtcta aatgaaaaaa attatgattt
    12721 atgttggtgc gtattggtca cgtgatccaa cagtattaga aaatccagag gcggtacact
    12781 attgcttgcg ccaattattc tatctgtata aagaacgatt agaatcgttg attcgtcagt
    12841 taccatatac tgacagacgt ttagatgaac ttcttctaag gtatccagca atgtataaac
    12901 gacgaaaaaa tagattgtta ccagaagagt atcccattga aaaacatgaa cttgaagggc
    12961 gatttgtggc ttatttctat gatgatgttc gtatgcgctt agtagaacaa cgaatggaaa
    13021 tagaaaatga tcgctactat tttatcaatt attgtaagaa gcgtaagtat caagtaactg
    13081 atgattttta tgattgtatt ttacaagatg gggaagtgat tctatccaaa attgctccaa
    13141 gttttcgtga acttgtgccg attgattttt tgaaaaaatg tcatttgcgg attcttccgt
    13201 aaaaaagagc tataaaagga aagaaaagta gaagccaaat cggtttctgc ttttttattt
    13261 tttttaggaa ggagaagatg gtatgtcact ggaattttta ggagagccaa ttgaaaagga
    13321 agagttcgtt tcgcattata tggatttgtt tattcaggtg ataaaaaact ttgtgcgatt
    13381 gatgaatttt atccacgcga acaagactat ctagccgctg aacgaataaa aatcagttta
    13441 ttatgggaaa aattcacaga tgaaaataac cgaccgccag attatcgcta tttatcagat
    13501 tgtttaacga ataatgtgct ttctaaagaa tacctatttc acaaacgtga gaaaaatcaa
    13561 atgtcggttg aacatttttg tgattgttat attgcattat tgcgtagtca acagttatta
    13621 acagtagaac gatatggtgc agagtttggt tatgtcttgc aagctgagcg tcatagagct
    13681 acaagatgct atgaaaaaga agatcaagtg attgatggct atgaagaatt atgtattcga
    13741 aacaatcgct atttgcaaca acgtttatat gaacaattga tgatggattt ttcaaatctc
    13801 tatagagggt atgaatgttc gatgattcaa gaaaggtaga agaaaagggg gaatttttat
    13861 gtggcgatta ttatttttgg tggtatgtgg tatggcattg ttctatgtgt atcgtgtcta
    13921 tctctatcca cgaatggaac gatggacaag ttggtataga gatcctttaa aacaacgaaa
    13981 aggattgaag cggtatcgtt atgaattact tgttgaattt tcggatgggc gacaagcaca
    14041 gtattatttt tttgcaaact atccaaaata caacaccagt gaactattga ttcaattatt
    14101 caaaaataat ccatatgttg ggttagaaga aaacggacgg gagttctttt atcgttcaga
    14161 taatattagt gcggcgtcta tgaacaaaac acgaattgca taaaaaattg gaggaataat
    14221 catgaaaaaa acaacattag cagtcgtaac aacactagga atttcattat tttcgtttca
    14281 attagggagt tcattggttt atgcagatga aactgtagaa acacctaagt cagaaattgt
    14341 agaaacattg ccaggggaag gattaccaga tgttgagaca gagctaaatg gaggaacaac
    14401 ggaaaaacca gcagaacctg aacaattgca gtcagagaaa cctgaaaaag agccagtgac
    14461 accagaacca gagtttcctg aatcaacttt accgactgat tcgatcgaag aagaaggtgt
    14521 gcatagttca acaacaacgg attcaagtat agtcacaact ccggaacaat caggaacaac
    14581 cagttcttct gaaaagaata ctgattcgtc aagtacggaa cagccaacga tgtcgtctac
    14641 cagtgaagaa caaaaagaag aaccgaaaca acctgttgaa aaatcagaag caattccaaa
    14701 agaaaataaa cctattgttt cagcaaaaga agtcacagta tcggtaaccc caagcggtga
    14761 aattacgacg aatacttctc aaggaatgag tgtaccgatt gtcactagta atgtagagga
    14821 attgacccat attccaacac caaccacccc attaaaagta gcaggtgatc aaacaattgt
    14881 tggtgtaaaa gatggtattc cattaatcca agatagtgaa gggaatttgg taaaggattt
    14941 gtcgattcct gtaaagaaac ttccaagtgg caatattgaa atcaaaacag cagatggaaa
    15001 aacaaaagtc ttaccaaaga ctggtgaaga aatacatgtt gcactttctg ttctaggagg
    15061 agtgttgaca agtattgctg gatttgttgg atataaaaga aagagaaaag gaatagaaaa
    15121 tgagggtaat tgaatggact atttttcaga aaaacaagct tatcgttgtc aatcgtacgt
    15181 actagtaaaa acatactaga aggagtagct tatgaaaaaa cgacaactac ggcggaaaga
    15241 aaaagtttat acagagaaag taccgaagca aaagaaaatc cattcaaaaa aagcagtgaa
    15301 atggatttgg tgtttggttt tatttctttg tttttcaggg ggcttggcat tttttcaatc
    15361 catgaaaacg aaggttcaag tgtcgttttt gaaagatgaa atccaacaat tacaaaagaa
    15421 agaagtacaa agtcggtcag aattaacgta cacacctgaa attgaatcct ttatgaatcg
    15481 ttttgttgct ttgtatatga atacattcga taatcatgaa caagaacaac aacgtcaaga
    15541 aacattaaaa aaagagtttt atgcacaaaa ctttattcaa gaaccaggaa gtgtttcagg
    15601 tgttcgaaaa ttaactgctg cttcgtttat tggattaaaa gaaattgaaa ccattccaac
    15661 tgctagttat aaagtaactt atgatattcg ctcgatgaat ccagagagta agaaagaaac
    15721 aagtgtgaca atcacacaag tactcaatct tcccttttct atttcaaaca agggctgtcg
    15781 tattattgct tatccttatt ttacggcact accaaagaat acagaaaaac aacgaatgat
    15841 tacttatgat gacacattat acgaaacggt tgatgcgtcg gaagcgaaag atgttgaagg
    15901 atttgtggaa gatttcttag ataagtatgc gaaaaacaat gcaaaagata tgtcatacat
    15961 gatgaaagaa cctgaaggac tcaatcaaac agctaaagtt gatcaagtag agatgaaagt
    16021 attgcgttat aaagaaacga aaaaaccttt tattgtaaaa gcaacggtcg tctttcgaga
    16081 tggtgatacg tcacttttac aaaaagaaca aatgacgatt gtcattacga aaaaaacaaa
    16141 tcagtattat gttgaaaaat taacacataa ttggttggat aggaaaggag aagaacggaa
    16201 atgacactga agggaattgt agattttata ttaacgcaag gtggctattt tttaataatg
    16261 gtggttgttt atctgggatt gcgaaatttt atgaaatcaa aagtaggaaa tgcattaata
    16321 tctatcgcag caggtgccgc agtttatttc ttcttaaatg atccaacacg tgtatttgat
    16381 gcgtttggta aaatcattga aaaattattt atggattagg aggatagtga agtggattgg
    16441 gaaccttatg attattcgat tgtttatgcg gaaccttatc gcgtacaaca gattacaagc
    16501 aattggcgtc ttccatttcc agttgcatta gtcaatgcag caactttttt gttggtcgta
    16561 atctttgaat atttattttt acgttcattt attggttggg tcatttcttt ttttccacca
    16621 gcaggagttg ttttttatat aggactcccc tatttatgtg tgttagcact ggcaaaacta
    16681 aaacctgatg gaaaaaaagt gcatttttat ttatgggatt ggttttgtta tatgattcgt
    16741 atgatgaatg gtgattgtgt gtattataac tgggacaagg tatcaaacaa tgcattggaa
    16801 gaattacttt ttgtgaataa gtatgtttat ttagaggatt agaaaaaggg acaatcaact
    16861 ggttgttcct tttttattgg gaggtgaaat tcattggtgc ttgtaaaaga accagtaaaa
    16921 gcattcaaga aaaattgttt gttaaatgga gaggattgta tctgggccta ctatcatgtt
    16981 tttccacgaa caatttcaaa aaacacacca aaaaagacac aggaaataaa agaaatgatg
    17041 gctcgtttac tgttgctttt agcagattat gaagatgtgg agttggtgat gtatccacga
    17101 acaatgaatt tacggacgaa gtttgacaca ttaggaaagg gattttctcc cgatagtcaa
    17161 gcaattggtg cgtattatgc aaaagaaaca gtgacattgt tggaacgaga aggtcaagtc
    17221 tatcgtcctt catttatttt aggaatcaaa gtcaaagaaa aagcaacggt ggagagtttt
    17281 catgatgtaa taaatctaat ctggttagat ttagcaggaa gtttagcacg cttatttgga
    17341 ttagaagtag atgatttgga aactttgttt tctgaaagtg atccttatgt aaaagaagtg
    17401 acacaattac tttatagctt ttctattcgt cccttagtaa aaaaagagct gttgtatctc
    17461 aatcgtctgc aatatattcg aggaatggct cacaaagtag aagaacaaaa taattgggaa
    17521 gtactgaaag acaatcaagg aacgtatgta cgtataccga aggaacataa aggccttctt
    17581 caattagaag atgagttagg gagtagttat ttagcattgt tgcctattgg ggaaacacct
    17641 gaaaatatga gaaattgtga tctatttcaa tttgctcagg atctttcttt cccagcagaa
    17701 ttgcgaatga aaattcacta tccacgcttg aaaggacagg gaggtattgg tttaaaactg
    17761 aattggctat cggaacggtt caaaacagag cataaggaat tggttcaaaa tgatgaagaa
    17821 cctagtgaac gcttattaac tgttcgagga ttagtaaaac atttaaagca acgagtagat
    17881 aatgggcaac ctgtcgtcga ttggatcgca tgttttattg tatacggtca aacggttgaa
    17941 gaagttcgtc aaaatagtgc aacggttatt caacaacttc atacgattat ccatatccac
    18001 cgtgcaaaaa atgatcaacg agaattgttc tataaatgtt tattagggca accattggga
    18061 cggcagttac attggaagca ccgaacaact gtagaagcag ttggcgaatg gttgttttca
    18121 gcagtatcag aattaggtat ggaatccggc tggtatattg gtcggcaaga tacattaggg
    18181 gatggtggtt ctcgaggaga aaaaacacca ttagaagaat taatttatgc gtcaaatcgt
    18241 tttgtctttt tgaatcttct agccattgcg gaaggaatca aaggtgcgtt gtttgatgcg
    18301 cctcatgtag cagtaactgg aaaaaccggt aaagggaaga cctttttagt tggcttgctc
    18361 tttttgtata gttcgtttat gaatgtacaa agtttatttg tggatcccaa aggagaaaag
    18421 cgcaaatggt ttacgaaact cattcaagac ccgtattatc agaaacatta ccctttgttt
    18481 gtagaacatt tgcaaaaaat gagttatgtc acgttagatg ctacaaatcc ccgtaattat
    18541 ggtgtattag atccaattgt ttgtttatcc cgagtagatg cgaaacaagt agcccaggat
    18601 atgattgatg agttaagtcc gttaggtacc aatcaccttt taaaaggggc ggtattaaag
    18661 ggaatccaag aagttttagt tcaaaaagaa gccggacaac aagttggctt gatgcatgtg
    18721 gtcgactatt tagaacaaat ggaacaaaaa gaggttcgag aatatggtga gtttttacgc
    18781 ttgacggtag aagattcgat tttacgttta ggattttctt atggagaaaa cccgggatta
    18841 gactttaatg ccaaaacaac gattttagag atccaagatt tgaaattacc aaatgacaat
    18901 gtccgaccag aactatatac agatgcggat cgaaagtctt tgtgtttgat gattagttta
    18961 ggtcgttttt gtgaaatgtt tggaaaacga gatagtagta agaaaacagc gatttatttt
    19021 acagaagcat gggtatttaa taattcgaac gcaggacgta gtattattgc agcaatggca
    19081 cgtgtggggc gttcccagat gaatcaatta gtgttagata cacaatttat tggtgactta
    19141 ggttcagata aagaaaaagg gaattttggt gtcgtctttg cttttgatga agacagtgag
    19201 cgagaaaaga ttttgaatca tttacgtttg gaagtaaatg aagcgaactt agctgaaatg
    19261 agccacatga ttaaaggaca gtgttggatg ttagatccct atggacgtgt aggaaaactc
    19321 aatgtacaca gtttgtttga agaaatgacc gctgcattac aaacaacgga aaaaacaagt
    19381 cactctcaag tagaagaaca ggatacgtta atcatgcaat agaaatgagg tgaaacaatg
    19441 gcttatataa agcataagga aaagaaatgg tgcgttttgt tttttttaac gattggattc
    19501 ggactgcttt ttgttggtgg attggtgtat gcggcagaaa agcaggtgga tcaatcgatc
    19561 tatgagattg ggaaatttca tattgatacc ttttcttctt atttatctga tagtgaattt
    19621 tcattaggca aaatgttatt tggggcaaat ggcacagatt ttctaaaaat gatgacgaat
    19681 atgttcttta gtttaagtaa attgatttgg caaggccttg attttgtcat tgagaagctc
    19741 tatgaaggag ctgcaatgaa cgagttaatt caccagtttt tcactttttc ggatactatt
    19801 tattcaaaaa tatacgatcc tattggtgcg gggattgtcg tcttgtatgt gttatatcta
    19861 tttactcttc gtatatttag agggaaacat tacgcaaaaa tggtgtttat tcgatttctc
    19921 ttcgttgtca ttttttcaat ggtttggttt ggctatggta gtaacgtacc tagtaaagga
    19981 gaagctttta ccaaagggtt aaatgatttg tccgtggagg tagaaggctt tattttttct
    20041 gctacaaata gtctagatga attggcgaca aaagatgtca atggtgtttt agtgccaacg
    20101 acacaaaagg aagccattca aaaagtgcgc caactttatt ataaagcggc tgttgttgat
    20161 ccgtatatat tgttaaatta tgggacaaca aatttagaag aattgtatca agcagagatt
    20221 gaaccacaag aatttctagc caaggaagcg tcaagtgaag aattatctga tattgagaca
    20281 aaagtgacca ctgctgcagg agaagataga gggaaaaaga aggcttatcg tgtctatatt
    20341 caaccaacta aagctgttta taaagcagtc gttggttcta tggtgccatt gcttaatcta
    20401 agtattggat taccacttgt agtgattggc atgattcgtt ttatctttca aatgttggtg
    20461 ttgtgtttat tacttgtgat tcctttttca ttactcgctt cttttgttcc agtatttgat
    20521 tatatgctca tgaatacctt aaagtccttt ttgggcttga tgtttcaaaa atctttttac
    20581 agtgtgatta ttttggtggc atttttgatc tataacatag tagacgattt aattccaatg
    20641 aatactgttt ttggtttttt aggaaatatg tttgtcaaag cactcctatc aattttactc
    20701 attgtaaaac ggaaagattt attaaagaaa ctagggttag gagctgcaga tcaaactata
    20761 aacaacacga aaaacatagc aagtcattct ttgtctcaaa tgaaacaact gcctggtcaa
    20821 ctatcaactg ctggacaaaa tttggcaatc aaaggtgcag gattagcagg gaatgtgtat
    20881 cgagcaacga atccactcgc ttctgcagcg atacatggat ttcaaaagtt tcaacaaaaa
    20941 cgtacaccac aagaatcggt aaaagggagg caagtggtta atccctttga actaggtggg
    21001 cgttcaaatc aagccttgcc ttatagtggt cgtcatgttc gttatcatgg ggtagagcct
    21061 gaaatgtctt ctaccaaatc gaaaacagag tggaattctg gtctttcaaa tggtttgatt
    21121 ccaaattcgt ccatgaattc agtaagacca aaacaacagg tacaatcatt ggcacaattg
    21181 agtaagcaac aagggaataa tcaaagaaaa ccattgttaa ataaaggtat cgcttttgtt
    21241 ggtcaatcga ttccgttgcc ttctggaaaa gtacccgtgc cacaattaac agctgctggt
    21301 tctctttcac aacagattcg ccaagtgaaa aatcaggcga aaaatgggag ttatccagca
    21361 atgcagcagt tattgaacca atactctgtt cctaattcgt cggtagccac tctatcagga
    21421 aatcgttctg tgcaacaacc ggggcaacgt tcgtctgaaa gtaaaccgtc gatttccgca
    21481 ccaaaatcgt tacaacctgc attttcttct ttagataagc caacagagca atcggaaaaa
    21541 atagaaaagg atcctaaaac actgccgatt ttggacgcaa aaccaggaac tgattttact
    21601 ttaccaaaag gacgaacagt ccaaaaacca ttatcgaaga aaggatgagg aagaaatgaa
    21661 gaaagggtgt ctaggttgtc ttcttttctt tattcttccc ttttttctat tgccattctt
    21721 tttttcagga gaactttctg agcatggagg agaagctaca ggtctagctg tcgcttttaa
    21781 tggggaatat accgaaggat taccgatttt ttcagagatt aaaggacgtg gtccaatcac
    21841 agatgaacat gcgcaagcag cagttggtgc agctgtaaag tatcagttat taccgagtgt
    21901 gattctttct caattaggct atgaatcagc ttatggacag tcacaatctg gaagaaacga
    21961 tacgaacttt tttgggatta cctggtttcc tggttgtccc tacccacaag gttctgcacg
    22021 tggaattggt ggttctgaag gtggtttcta tatgaagttt ccgaacttaa aagcttgtta
    22081 tgactattat ggctttatgg tggcgagcca aagcaacttt aatcaatgtg tgggcaataa
    22141 agatcctgga agttgtttat tgattctagg tcgtggaggc tatgcggcag caggtattac
    22201 cgaagggagt ccgtattatc gaggagcaat gagtatcata acgtcctata acttcacgga
    22261 atatgatact tttgcgatca agcattggaa tagcctacca acaaaaaaaa cttcgcttat
    22321 tgcaggaaaa agtaaaggca atgcgaaggt tttggatcgg atcattggac gatccgtaaa
    22381 taatggggaa tgttatggtg gtacggcatg gtatgtatct caattaggtg gcccacagtt
    22441 aatgggttct ggacacatgt ttgcgatgtt aataggggaa gattataact ggacaaccta
    22501 tggttggcaa gtaatcatga acccaaaacc aagtgattta caagcaggag atgtggtgaa
    22561 ttggcaagca ggtggtgcat tgtctcctgg aatctatggt cacaccggaa ttataacaga
    22621 tgtttcatat ggcggacaac atttctctag tgtggaacag aatgccgagc aaggacggat
    22681 tatggctcgg tatcaacgca cgtacaatca aacaaaaatt cgtagtctgg ttcgtaaggt
    22741 gaaataagaa aggcagtgac tagaaacgtg aaaaaaggga taattttagt atttagttgg
    22801 ttatgtttga taggtcttac tggttgtcat tggttttctt ctgaaaaaca aaaaacacaa
    22861 ttgagaacga cagaagaaac aatcgaagca gtaaaagaaa caagtggaac tcttcaaagt
    22921 actgaaagta acagtcaaat tggttcatta gaagacgaga cagcgcaaca aatgcaaata
    22981 gatgaaatga caaaaaattt cttaaaagat tttgctagaa aatggagtaa tttcacagat
    23041 gtttataaac gcaatcaagc tgtgagggac tatatgacag atcgttgtat taaagacaat
    23101 agtattgatg tagacccgca tgtacaaatg aaaacaactg ggaaacttta ccaggtgaca
    23161 caagatatag agaatccgga tcaatatttg ttatttggag aagaaacaat gaataaggtt
    23221 actcaatttg tattacttca attgaaagta ggtcaagaag ctcaaaagat tgatgcgatc
    23281 aagatttatt atgtacggag tgcgtattaa aataaaaaat gaacactcat atttattata
    23341 caccgaagaa atagaaaaaa acagactgtt tttttaagaa aaactatgtc attctttact
    23401 tgaaaaaagt tgttatagga cagaaaggat aaaacaaaaa cggaaaaaat atgtcttatg
    23461 aataactata agaactattc tattttttag gagatggata cgagatgaaa aaactaattg
    23521 gctatagtgt gatgtgtatt ggatttgttg gtgtgggatt gtttgtatcg actgaatctg
    23581 cttctgcggc tgaatggtat aatcctatct ggtataatcc gaacattgaa tgtcaaaaag
    23641 gctatccgta tggaccgaca aatctgaaaa atggaaaatg tagagtaaat tggaatggcg
    23701 ttgtagatag tgtaattact ggttcaatgg actctgctct acatggcttt acgggtcact
    23761 agaaggagga agaaaatgtc tgaagagaaa aaagaaatcg ttgaatcatt agtagcttta
    23821 aaagattctc tttctaagat tgaatatgtt gataggcaag aaattcaaaa actaattgat
    23881 gatactatta ttgaaatcca agatgcgcgt tgtgaaggta taaaaatttc tgtagcttta
    23941 tctaaagtaa tagaaaaaat gaaccgttca ttagcgttta atggattgaa gctagataga
    24001 caaactagtc tgatatggga tcatttaaaa gacttgtatg acaagtctaa aagaagtgaa
    24061 cggactgctg tatcaatctt aaaaggcctt tggggaatga attcataaac aagatattta
    24121 aaaagtaact tattttttat ggaggagaaa ttatgaaaag aataattaaa atatttggtg
    24181 ttttaggtgt tttcttatta ggtttaagta cttttgttag tactggaaca agtgtgtatg
    24241 ccacagaaaa agtgaataca gttgatttag aaataaagaa taaattagta gatgaattaa
    24301 ctttttattt tgatacagtt ggttttttag acgaacatgg tgtataccat ataaaggatt
    24361 ttggtgctat tcgtgaacga atcagtcaag ataacgaaca aggaagagca gcaagagtct
    24421 tttatgaaca tattattgtt cctagaatgg agaaagttga ttggaaacga tatgctggat
    24481 gtgtagccat taattctctt cctggaggag gagtggcttg ggctataggt acgcagggag
    24541 attttattga agcattaaaa gcttttaatt ttgaaaaagc tgcggattta ttattaaaaa
    24601 ttgcagaaaa acaattagga aaagctgcag ctaaagagtt agctaaggta aatattgtag
    24661 caggaattgc agcagctgct gctagctgtg cattagagta aaagtttatg aaaagtttag
    24721 aacaagagca tatggctttt aaacaagcaa tgtttaaaga aaatatctat ttaaaccata
    24781 actatattcg tgtttctaag gcatgttcac cagtattgaa tatgcttgga ggtggaaatg
    24841 gattatatca tttactgttt gtcgatgttt gttggttaat ttttttacca gatgaactag
    24901 ttatagtcaa tgaaaaaata acaagtaaaa atgaagtgtt taattattct ttaacaagaa
    24961 ttaattacaa agagattacc aagttttcac tagaaaaagt tcctttttgg ggagaatttt
    25021 gtttaaaaat taaatgtaac tggaaaagaa tgtatttcta tattgatggt gatgatgctc
    25081 ttacattcgg taaaactact ttttctagtt ttaattttca gtttctatta aaaaataatt
    25141 tttatggttt attgaaataa ggttgcgaag gaagatgtag agttatttgc actgttgtgt
    25201 tggtatatct cttaatgctg tgtctttttg gggagattaa ttagtggagt gattatatga
    25261 gtatagatga ttgtttagaa gaacaaataa gattattttt aaagttttta aaagttaaag
    25321 ttcaaaataa ctacttcaca tgttatagtt atgcacaccc tgttttaaat agtcttcctt
    25381 ttaattcttt agtgcaagga ttgattacaa atattgtttt attagtctgt acaccaaatg
    25441 aaatatttgt aaaaaagata ggcagtggta tttcttcagt aagattaaaa gagaaagaat
    25501 atactcaaaa gattattcat attcctaagc ctgaaattaa gcgttttgag ataaaagact
    25561 attctgcttt agggctgttc tcaattggtt actggtgata aaaaataaga atactaaaac
    25621 tatacgtaga atataagtaa tcggcatcaa tcctaattat gtgattgaat gccgattttt
    25681 gttacgcgct atttttaatt atctgtaaga agaataattt tattttactc atattcgttt
    25741 tcactttgaa gaagtaaatt gcaatttctt ataagttaag cagctatgca aaaggctatt
    25801 tattctattg tgaaatataa gatgtagttg aaacaggata tatagaatca aaatacgaat
    25861 cttattttga tttttctagt tttttgaaaa aagcaccctt tgatcgttgt attagtggaa
    25921 aagagaaaaa ggagcatact tatcatgtca caaaactaca actaaatatc taaacaacac
    25981 caatttgatg cctattgaaa aaaagtattg cgaaatgaag ccaagtctat tagaaaatga
    26041 aataatagaa taaaagaaaa tgggaaaccg ctgaatgatg taaatgaagg taagtatcca
    26101 cgaactaatt ttgatgaaca agatatctat tttttatttg tattggagat actagtttaa
    26161 tatcaaaagt tatcagcagt cataattagt tatccaatgt aaaaagtaca aacaatagtt
    26221 gttatgcaga at
//
LOCUS       DAYYHS010000037        25580 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000037 DAYYHS010000000
VERSION     DAYYHS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.037"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..597
                     /locus_tag="ACHA6A_001485"
     CDS             43..597
                     /locus_tag="ACHA6A_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8969688.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(770..1243)
                     /locus_tag="ACHA6A_001486"
     CDS             complement(770..1243)
                     /locus_tag="ACHA6A_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8969689.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKIKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1255..2202
                     /locus_tag="ACHA6A_001487"
                     /pseudo
     CDS             1255..2202
                     /locus_tag="ACHA6A_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2473..2908
                     /locus_tag="ACHA6A_001488"
                     /pseudo
     CDS             2473..2908
                     /locus_tag="ACHA6A_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            2924..3757
                     /locus_tag="ACHA6A_001489"
     CDS             2924..3757
                     /locus_tag="ACHA6A_001489"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8969690.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4002..5027
                     /locus_tag="ACHA6A_001490"
     CDS             4002..5027
                     /locus_tag="ACHA6A_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8969691.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5165..5290
                     /locus_tag="ACHA6A_001491"
     CDS             5165..5290
                     /locus_tag="ACHA6A_001491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969692.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5315..6430
                     /locus_tag="ACHA6A_001492"
                     /pseudo
     CDS             5315..6430
                     /locus_tag="ACHA6A_001492"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6513..7409
                     /locus_tag="ACHA6A_001493"
                     /pseudo
     CDS             6513..7409
                     /locus_tag="ACHA6A_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7425..8249
                     /locus_tag="ACHA6A_001494"
     CDS             7425..8249
                     /locus_tag="ACHA6A_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8969693.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8281..9591
                     /locus_tag="ACHA6A_001495"
     CDS             8281..9591
                     /locus_tag="ACHA6A_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8969694.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9739..10998
                     /locus_tag="ACHA6A_001496"
     CDS             9739..10998
                     /locus_tag="ACHA6A_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8969695.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11008..11880
                     /locus_tag="ACHA6A_001497"
     CDS             11008..11880
                     /locus_tag="ACHA6A_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8969696.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11892..12722
                     /locus_tag="ACHA6A_001498"
     CDS             11892..12722
                     /locus_tag="ACHA6A_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8969697.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12762..14945
                     /locus_tag="ACHA6A_001499"
     CDS             12762..14945
                     /locus_tag="ACHA6A_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8969698.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15006..16226
                     /locus_tag="ACHA6A_001500"
     CDS             15006..16226
                     /locus_tag="ACHA6A_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8969699.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16223..17683
                     /gene="gtfA"
                     /locus_tag="ACHA6A_001501"
     CDS             16223..17683
                     /gene="gtfA"
                     /locus_tag="ACHA6A_001501"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8969700.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17760..18866
                     /locus_tag="ACHA6A_001502"
                     /pseudo
     CDS             17760..18866
                     /locus_tag="ACHA6A_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19188..19397
                     /locus_tag="ACHA6A_001503"
     CDS             19188..19397
                     /locus_tag="ACHA6A_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8969701.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19390..19539
                     /locus_tag="ACHA6A_001504"
     CDS             19390..19539
                     /locus_tag="ACHA6A_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8969702.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19693..20358
                     /locus_tag="ACHA6A_001505"
     CDS             19693..20358
                     /locus_tag="ACHA6A_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8969703.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMSRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20642..21727)
                     /gene="xerS"
                     /locus_tag="ACHA6A_001506"
     CDS             complement(20642..21727)
                     /gene="xerS"
                     /locus_tag="ACHA6A_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8969704.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22061..22294
                     /locus_tag="ACHA6A_001507"
     CDS             22061..22294
                     /locus_tag="ACHA6A_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8969705.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22291..22698
                     /locus_tag="ACHA6A_001508"
     CDS             22291..22698
                     /locus_tag="ACHA6A_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8969706.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23835..24353)
                     /locus_tag="ACHA6A_001509"
     CDS             complement(23835..24353)
                     /locus_tag="ACHA6A_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8969707.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            24720..24968
                     /locus_tag="ACHA6A_001510"
     CDS             24720..24968
                     /locus_tag="ACHA6A_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969708.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            25471..>25580
                     /locus_tag="ACHA6A_001511"
     CDS             25471..>25580
                     /locus_tag="ACHA6A_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8969709.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         8710 a         3918 c         4779 g         8173 t
ORIGIN      
        1 cataatcatt aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa
       61 ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat
      121 ccggaaagag atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat
      181 attataggat taaaagttgg agatatacga ggcaaaagta agcctattat tgttgaacat
      241 aaaacaggta agcgaaaacc gatttttata gataatttga gagaagaaat tttattatat
      301 actgaaggta aagaagaaaa tgattggtta tttccgagtc ggcaacaagg tcgacatata
      361 acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg acgagatgat
      421 ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa aactagagac
      481 attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt
      541 ggtataactg aagatgaaat aggagcatca cttcgtggat ttaaactagg tgtataattt
      601 aaaaattttt ataaacagta aagacaaaga ttaaaagcta tttttatcta gaacgatttt
      661 atttcataaa gttcgcggaa taaaagctgg ggttactggt acttggtata ggttcgtgtt
      721 atataaacta agtaattaaa taacattttt aggttggaaa aggatgaaat taaaaaaaga
      781 gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa gaaaaatact gataactcaa
      841 cgtttagcag actatctaat agggatagtc tgcttttttc gtttttgacc atacttttga
      901 tcttctgcaa acttccttgc ttttcctctg tagaactcat gaaatctgca aaacgaattg
      961 ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat atcatccata gtggttttaa
     1021 tatctgtatg tccaagtcga acatacactt ctttgacaga cgcaccagat tcaaagagca
     1081 gactacaatg ggtatgacga aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa
     1141 tgatgatttt caaattgtga tttacatagt ctaaataaag gggcttattt tcttctgatg
     1201 aaaacaaaag ttgttcacca tcttttatag gtttgattct catttttaga tattatgccc
     1261 gcacgtattc atcacatttc tattgtgaat cgctttattc gaccgacatt tgacttttat
     1321 cataatatac tcggtttaaa attactaatg aaaacaatta atcaagacga tcataccatg
     1381 tatcacctgt ttttttcaga taatcaccac cgtgtgggaa ctgaactgac tttttttgaa
     1441 gtacaagaag ggaacaacca gttttttgga accaatacga ttgaacgaac gatactaaaa
     1501 gttccttccg aagcatcatt gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc
     1561 cattatggaa tcgaatcatt ttctggacga cctatcttac gttttgaagg gccagatgca
     1621 acacgattag ctttagttcc cttacgcgaa tttgaagata ttgatgatta tttcccgtat
     1681 gtcacagacc agatccctac agagcatgcc attttaggaa ttgatgccat tcaactacgc
     1741 gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg taggctggta gcacaaagaa
     1801 tacgtcagtt tcttttcggg tcatcaacga gtgacaatct tagaaaatca agacactcag
     1861 ttctatcaag aagttcagat tattcaagat cgtgagaatc cagttgccat tgaagggatt
     1921 ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat ctgatttaca acgcatcgac
     1981 aaacaattac aagagcgcaa ttttataaat tcaggaataa aagatcgtga attttttgta
     2041 tcactttatt atcgtgaccc aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac
     2101 ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg aagaaattcc tttattttta
     2161 ccgcagtact taaatttcat tcgtgaacaa gtggagcaat aattagctaa tcaaatccat
     2221 gacgaagagt aagaggttga ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat
     2281 tctaaagaat gttgatttga tgcggttcct atttttataa actatcattt tcgactaata
     2341 aaactactta tgagtcagcc tctctttttt tcttgatttt tatacctctt tctaggtaaa
     2401 ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa
     2461 ggggagtacc aaatgaacga gtatattgaa attatccacg caagagaaac aacttaaaaa
     2521 tgtcagtctc aaaattccca aaaataaaat tagtattttt accagcgtat ctggttcagg
     2581 aaaatcatcg attgtttttg atactattac ccaagaagct ggtcgtcaac tgaatgacac
     2641 ctatgatagt tttacccgtt tatttttacc taaatacagt cgtccagagg ttgatgagat
     2701 tgataatctt tctacggcga ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc
     2761 aacgttaggg acgattacta acatcaatgc gttatttcgt gtgttgtttt catgttttgc
     2821 aacacctagt ttaggatatg caaatgctta ttcatttaat gatcctagtg gaatgtgttc
     2881 tgtctgtcag ggaatcggta agacgattgc atttttaata ggattgcttt atttttcggc
     2941 tattgataat gctgattttg tattttctga tccatttacc gcagaagttt tgtctggaat
     3001 tgaggaacag gcaaagaatc atggttattt tgtattggtg cataatgtta attcagcaga
     3061 agatgttaaa actattcaga gaaattggcg ttttgagggg ttcattgttg ttggtgtacg
     3121 tgtaaaagaa tttttggaat taaataacgt ttttgatggt cctgttgttt atattgatac
     3181 gcatctttca aaagagacat taagagaact tgaacaacaa atgaatcgtt tatttgtaaa
     3241 tacaaatgat taccaagcta gtacattggc agtggaacat ttgattcatt atggtcatga
     3301 gaaaatagcg tttctttctt ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg
     3361 acatcaagct tatatcgata cattaaagaa atatcattta acctttaaat cgcattggat
     3421 atataacaac tctgaatttg agcgaattta tcaagaatta gctgaattta ctgcaattgt
     3481 tgttactgga gattatttag cggctaaatt agtaaaattt ttaaaaaata agaataaacg
     3541 agtgatggaa caattatcga ttattggatt tgatgatatt acttttgcag aattaatgga
     3601 tcctgcgttg acaacaatac gactaaatcc aggtaacaag ggggcgattg cttttcacca
     3661 actgattgga ctggccaaac aagaagaaat aaaaaatcaa gtactactat tagagggaga
     3721 attgatttct cgagactctg ttcacaaaat caaataaaaa agctctgtga aataagtata
     3781 gttaacaata aaatatccga acgatagaga gaatgaactg tgatctagca gttcctcgtc
     3841 gcaaattcac tagaattagc attatttact ctaaattgtt cggatatttt ttcttgtttt
     3901 taattatgat tacagtgcct atttttaacg aaatttgctt attttcaaaa tgtatgatta
     3961 tatgttagta aacactttca aatatgaaaa gaggagctat tatggtagga attagggatg
     4021 ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg cgtgctaaat gaggatccga
     4081 cactttcagt aaccgatgaa accaaacgaa agattgcaga atcggttaat ttttatcatt
     4141 atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat tgcattaata acgacagttt
     4201 ctgaagtaga tgagttggaa gatccctatt ttcgaacgat tcgcagaggg attcaagtcg
     4261 aagcagaaca aagcaaagtc aatttgaaga aaattattcg tttgtcagaa acaccgttgg
     4321 agcaagacga tttgctaaaa tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg
     4381 tgattgaaga agtatcagca attaattcga atgtcgtagt gattgatgac cctagtattg
     4441 aagctgaagt agatgcagtt tatactgatt taacaaaagc aacgaatcag catttagatc
     4501 gattgtataa aaaaggacac cgaaacattg cgtttatcgg aggaggacgt gttcgtttag
     4561 atagccaagg aaataaacac gtgtcagatg atgattatcg aaaaatagcc tatgagacgt
     4621 ggatgaaaca aaaaggatta gaaaaatata gtcaaattta tttagaaggc tggaccacac
     4681 ttgacggaat gcaggcagca gatttacttt tgaaaaaaca tgctaaagat aagttgccga
     4741 ctgctatcat ggtaggaaat gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa
     4801 atggtctaca tattccagaa gatatttcga ttgtgagttt tgataatatt gaagttgctg
     4861 aatttttaac acctgcatta aatacggtga atgtgaatac agaagaaatt ggaaaactag
     4921 cagttcgcat ggttaatgaa cgaataaaag aagttagaaa tgtttcgatc aaagtgattg
     4981 tttctaataa tattattgtt agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc
     5041 caaaaaaggg tgtttacttt tttgtttact aatattttta gtaaaaaatc tcgacaaaac
     5101 attaagtaaa cagttatggt gttgttaaca aaaagaaaac gcattcaaaa caggaggaat
     5161 taagatgaaa catttcaaaa aaatagcttt aacagggata gctgttttaa ctgggacatt
     5221 actcgcagca tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa ctttaagcta
     5281 tgcgagttga gatactgacc aagctccagg actaagaaaa atgttagatg aatttgaaaa
     5341 acaaaatcct gatatcaaag tagaaatgga gacaacgcct tgggatcaat attgggttaa
     5401 attagaagct gcaacaacgg gaggtaatat gccagatgta gtaaccatgc actctagcga
     5461 atcctataaa tatatgtcac agaatgcttt gatgggactt gatgatgtta tcaaagaaaa
     5521 tgatattgac atgggaaact ttactgaagg aattgctgat ttctatacgc ttgaagacga
     5581 actatacgca atccctaaag atgcttctgt agtggggcta tggtacaaca aagaattatt
     5641 tgataatgca ggtgtcgatt atccagatga aacatggaca tgggaaacac tcagacaagc
     5701 tgcaattgat ttaacagatg aatcaaaagg catctatggt tttgcatcac atattggaac
     5761 agagactggc tactggccat ttatttatca aaatggggga gaagtatttg ccgatgacaa
     5821 tacaaaatca ggtttgaata ccccagaagc acaagaagct ttagcttttt atcgagatat
     5881 tgcacttgtt gatcatgctt caccaacaat tacagaatta caagatcctg ataagttaag
     5941 tcgttttcaa tcaggtagat tagcaatgat gattgaagga aactggtttg caccgggatt
     6001 tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt
     6061 taatgcaaca attacaaatg gacttggttg ggctgccagt gcgagtacaa aatacccaga
     6121 agaagtgaaa aaactaatgg catttttagc tagtgaggac gccaataaaa tccaagcgga
     6181 aacgggggca tcaatcccag cactaaaagg ctttgaagac gagtgggctg cttcttcaga
     6241 tacaattgat ttgagtgctt ttggaaaaat gttggagtat ggagtccctc gtccatacaa
     6301 taaagatgga ttacgagctg aacaagcaga agttcaaatt attaaccgta ttctgtctgg
     6361 tgaagtaact gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg cgatattgag
     6421 cggaaattaa attagttaat tatacaatat acaggcgaaa ggattctatt tcgtctgtat
     6481 attaatggat gaataccaaa aagggggata ttatgaaaaa tcgaattttt ggaaaatatc
     6541 gtaaagatca agtgatatgg ggctatattt ttatttttcc aacggcatta ggattaattt
     6601 tattaaatat atggccagca attcaaacag caattttaag ttttcaaaaa acggtagggt
     6661 tcggtgatac tgaatgggtc ggattaagaa attaccaaaa aatgtttgca gacggtgaag
     6721 tttttcagtc tttattaaac acattaattt atgcagtggt ttcgatacct agtattgttg
     6781 tattatcctt gttggtggca gttttaatga atagaaaagt taaaggattg tcgatttatc
     6841 ggacaattta ttttctacca gtcgttgctg caccatccgc agtcgcaatg atttggcgct
     6901 ggatgttcaa taatgattat ggactgatta ataacatgtt ggctaaaata ggtctcgatg
     6961 gaccagcatg gttaacggat ccatcgattg cgatttattc tattatcatt gtaggagttt
     7021 ggagtgctat tggttataac atggtgttat tactagccgg cttataagaa attcctaaag
     7081 attattatga agcagcggaa attgatggtg ctagtccaat ccagcagttt ttcaacatta
     7141 cgttaccatt agttacacca acattatttt ttgtagttgt aacaacagtt atcaatgctt
     7201 tccaagtatt tgatgttatt tttatgatga ttactcctaa tagtaccgcc atgtttaaat
     7261 cacaatcatt agcctattta ttctataaac attcatttat tttaaatgac aagggatatg
     7321 gttcggcaat cgttatgttc cttttagtga ttatattaat tattactgca attcaaatga
     7381 agttgcagaa aaagtgggta aactattaag gaggctatca aattatgaaa aataaatttt
     7441 tttcaaatgc gccaatccat atcatgctta ttttaggtgg tattgcaatg gcattacctt
     7501 ttatttggat gattttaact tcagggaaaa cgttggttga atctacccaa attccaccta
     7561 caattttccc tgagaaattt cagctaagta actatcaaca agtacttgat ttattaccat
     7621 tcatggcttt ttatatcaat acatttttaa tggttgttgg acgagtgatt ggctcggttt
     7681 tattttcagc aatggctgca tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc
     7741 ttttcagctt agtacttatt caaatgatga ttcccggttc catttttatt actccacagt
     7801 atttattagc acaaaaattg ggtattttaa atacaattcc agcattaata ttaccagggg
     7861 tagttagtgc gtttggtacg tttttattaa gacaattttt cttaggttta cctgatgaat
     7921 tggaggaagc ggcctattta gaaggtgcca atatttggac gatctttttc aaaattatgt
     7981 tgccgcttgc taaatcagga ttggttgcac ttggaatatt cacagcgttg tttgcattta
     8041 aagatttaat gtggccattg attgtcaata cttcaattga taagatgcct ttagctccgg
     8101 gattagcaaa cttacaagga caatatgcca cgaacttccc acaattaatg gcaggttcgt
     8161 tattagcaat ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag
     8221 gaattgctac aacaggtagt aaattataaa actattaatt agtgattgga gagtaagtac
     8281 atgacaaaaa ttacatttat tggtgcagga agtactattt ttgcaaaaaa cgtattaggc
     8341 gatgcgatgt taactccaag tctacaagat gcacatattg cattgtacga tattgatgaa
     8401 aaacgactaa aagaatcaaa tttaatgctt aaaaatatta ataaaaattc gaatgaaaat
     8461 cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt taagagaggc tgattttgtt
     8521 atcaatgcta ttcaagtggg tggttataaa ccttcaacag tgattgattt tgatattcct
     8581 aaaaaatatg ggttgcgaca aactattggt gacaccatgg ggattggtgg gatttttaga
     8641 accctacgga cactaccagt aatgttagac tttgctaaag aaatggaaga agtttgtcca
     8701 aatgcttggt tattgaacta tacaaatccg atggcgatgt taaccatggg tgttttaaaa
     8761 gcgacgagta ttaagactgt tggtttatgt catagcgttc aagtatgcgt accagaattg
     8821 tttgagcatt taggaatagc cgatcaatat gacttaaatg actttcaatg gaaaattgca
     8881 ggcattaatc atatggcatt cttgttggaa attacaaaaa atggggaaga tttctatcca
     8941 acaattcgtc agttagctgc agaaaaacca aatccacatc cagattctgt acgttttgaa
     9001 ttgttgaaac gttttggtta ctatattaca gaatcaagtg aacacaatgc agaataccat
     9061 ccatacttta ttaaatcaaa atacccagaa ttgattgacg aattagggat tccgttggat
     9121 gaatacttac gtcgttgtga acaacaaatt aatgattggg aaagcatgcg agaggacatt
     9181 gtaaataatg gggagttaac acatacgaga agccgtgaat atgcttcata tatcatggat
     9241 tctattacga caggaactcc gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc
     9301 acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg
     9361 caaccaacct atgtcggtga attgccaaca caattagctg ctttaaatcg gacgaatatc
     9421 aatgttcaag aactgacagt agaagccgct ttaacactag ataaatcaaa aatttatcaa
     9481 gcagcgtatt tagatccaca tctatcatct gaattgtctt taacagatat caaaaattta
     9541 gtggatgatt taatcgaagc acatggggac tatttacctg aatataatta aaaagaagct
     9601 gaaagagtaa ggaattttca attccttact cttttcttag ttaaatttag taaaaatatt
     9661 gacaaaacac ttagtaaaca gttacattag tgttaacaaa aagaaaacgc attcaaattg
     9721 tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg tatagcacta
     9781 ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta
     9841 gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga gattgctaaa
     9901 gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc gaatgctggt
     9961 gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa tctttatcct
    10021 caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt aaccgaagaa
    10081 ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga ggatcgtgta
    10141 tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac tgcttttgag
    10201 gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt agctgagatt
    10261 aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac tttaaatggt
    10321 tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa caatgtttgg
    10381 cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa agattttaat
    10441 cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg tgccggttat
    10501 aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg
    10561 gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt tccttttcca
    10621 gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt atcagtttct
    10681 gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac aacacctgaa
    10741 gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag
    10801 aatactcagg attcaccatt aagtggttta actgaacttg cttttactga tcgtcattta
    10861 gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac aacaaattat
    10921 ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt taatccaatg
    10981 aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa tcgttactgg
    11041 gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta ctttccaatg
    11101 gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa ttttgatttt
    11161 gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa atcgattggc
    11221 ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg tattttgatt
    11281 gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg gttctttttt
    11341 ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt taactatggt
    11401 ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct tttaggaaca
    11461 acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat tgccatgcca
    11521 gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga agctgctaga
    11581 atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta cttattacca
    11641 agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt tgatcaaatt
    11701 tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt acttgtttat
    11761 aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt agtcttattt
    11821 atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt tgaaatctag
    11881 gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg cttttattat
    11941 tagggggctt catgatttta gttcccttag tttcaacgct attcggttca tttaaaacaa
    12001 cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc
    12061 gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc atcacaatca
    12121 ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta gcaagaaaaa
    12181 tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga atttttgttc
    12241 ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt ttagcaaata
    12301 tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta tttttatatg
    12361 ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa attgatgggg
    12421 ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca atgcatgcga
    12481 cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca ttattaattt
    12541 taaatcgtga ttctaatatg tggacactac cattgtttca atataattat acgggacaat
    12601 attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc acgattacac
    12661 tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga gcagtgaaat
    12721 aaccaataaa ttattctcat tataaaggag ttcataaact tatgattata tttgacaatc
    12781 acacacaaac atttcattta caaaataaaa aaattagcta tctattagga atcgaagaaa
    12841 ataattattt aacgcatctt tattttggaa aaaagattac gcactattct ggtgggtatc
    12901 gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat
    12961 tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat tttagagaac
    13021 cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct
    13081 acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat acggaagatg
    13141 atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga ttgactttac
    13201 agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg caattagtaa
    13261 ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt gattttccaa
    13321 atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt caaatgacta
    13381 gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca agcagccatc
    13441 aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat
    13501 atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa gatccatttg
    13561 accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa ctaaaaactg
    13621 gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt ctgaatacgt
    13681 tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac tatcgtttaa
    13741 aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt aacgaagaaa
    13801 aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt gttttagatg
    13861 atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg gttgaaacag
    13921 aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat aacgggatga
    13981 aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta tttcgagcac
    14041 atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga aatcaatatg
    14101 ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg acggcgattt
    14161 ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg acagaagtct
    14221 attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat atgttagggt
    14281 tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt gaaagttgtt
    14341 cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa acttggacga
    14401 gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc ttagtgtatc
    14461 caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa
    14521 cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat gagctagatc
    14581 taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc ttttataaaa
    14641 agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg tttgaaggca
    14701 atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc ttttatttcc
    14761 gagtattagc agaagctgcg catccattag tcacattgaa attagcagga ttagcagaag
    14821 aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg aatctcggtt
    14881 tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat ttgaaacaga
    14941 tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt ccaaacgaag
    15001 aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat tgggaaaatg
    15061 aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa ggactaggcg
    15121 gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc atctttgaat
    15181 ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag tcgggcattg
    15241 aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt atgacggtag
    15301 cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat ggctattatt
    15361 cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt gaatggttta
    15421 aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat caattagtta
    15481 cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca cgttcaatta
    15541 ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg gcgacgcttt
    15601 ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga atgaccaaca
    15661 tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag tataaacggg
    15721 cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg
    15781 ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc tcaacttctg
    15841 ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa gctcagttaa
    15901 ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga aaaaatagtg
    15961 tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa aatgaacata
    16021 ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta aacgttacag
    16081 caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc aataattatg
    16141 aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa agtattgtat
    16201 tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc
    16261 agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac atttacaagg
    16321 tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc gaggctttgc
    16381 gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg tggaagcatt
    16441 aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc gtgaatcaaa
    16501 atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa tgtttatccg
    16561 tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt taatctacaa
    16621 gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga cagaagaggt
    16681 ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag ttgtgaaaga
    16741 atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa ttcgattaga
    16801 tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg aaccagatat
    16861 ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc
    16921 agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt attatatcta
    16981 tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat cagaacgttt
    17041 agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata cgcatgatgg
    17101 aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt acacctctga
    17161 ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca attacaataa
    17221 tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata atgaccgtag
    17281 ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa tttattacgt
    17341 tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag aagggcgtaa
    17401 tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac ggccagtgat
    17461 tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg atttggatgg
    17521 aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta aaaataaaaa
    17581 cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta tcattgagtc
    17641 taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc catagaacaa
    17701 aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag gactcataca
    17761 tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata aaattaggaa
    17821 aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt
    17881 cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc
    17941 gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat gtgtcatcga
    18001 ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg ttagaagaaa
    18061 tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag cgaaatcatc
    18121 tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag tgggcgactt
    18181 tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg gacgatacta
    18241 cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc aatcaactac
    18301 cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat taggactttg
    18361 aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt aaaaaaaaca
    18421 ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt ctacgagacg
    18481 agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta gtcactgttc
    18541 aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc tcttcaaaag
    18601 aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag cacatcaaac
    18661 aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga
    18721 ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg aaaacggaaa
    18781 aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc tttgaatgtt
    18841 gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac ttttggtttt
    18901 cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc agcacagttt
    18961 tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca
    19021 ctagtgatat tttttagaat tagatttcta atgtgcattt tttgagtatt aaggagcaag
    19081 tatacaaaga tttataaagc tgattaaatt tgaataaaaa tatgcaggaa cgctactatt
    19141 tttagtagtg ttctttttta tatttgaata aaaaatgtgg tggttaaatg gtggttataa
    19201 caaaaaatga attaatgaaa ttaggttatg ggaagtatca gtctgagaga atcatacgac
    19261 aggcaaaagc aattatggct tcaaaaagct atacctatta cgaaaataga agattaggac
    19321 tagtacctgt tgaatctgta gaagaagttt taggtatgaa gatagaggta gaggaggatt
    19381 ttcagaatca tggttaattt taaaaaagat aaaaatggga aatatcattt tactgctagt
    19441 ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag
    19501 ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat tatggaaaag aagcagaaac
    19561 ttctcaaagt actatgttgt ttgaggagtt tttttataaa ttattcctac cttattatca
    19621 agggaaagta gaagaaagaa catataaaaa tcggttatct acaatcaaaa aaacttttct
    19681 tatttcttta aaatggagtt aagagaaata cgtccaattg acattcaaaa gtggcaaaat
    19741 aatttattga gcaaatataa aaattcttat gtaagaaaca tgtatgggat ttttcagatg
    19801 tcattagatc gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa agttattgga
    19861 aatgtgaaga aaaggaaaac tacagttgat ttttggacaa aagaagaatt tacaaaagtt
    19921 atttcaactt tttatattga tgatttgtac caacattttg gttttatttg tatgtggtta
    19981 ctttttatga ccgttatgcg tttgggagaa gccactgcat taacttggaa agatattgat
    20041 tttgaaaata aaaacttgcg tgtgaataaa acactctatt ataagagagc caaaacctat
    20101 gagtttgtag aacccgaaac ggcttctagt attcgtacaa tcgaactaga cgacaataca
    20161 attacctttt taaaagcttg gaaggagcgt caagtttcaa taggaggcat gtcgtttgta
    20221 ctttcttata atggaacacc tactcagaaa tatacaatcc gtcatattat gagcagacat
    20281 gcaacgatcg ttggtgttca tataatccgc atacacggat taaggcatag tcatgcttct
    20341 ttgcttatat cacaataaaa agatgcactt cgaattaaag aaaggttaaa acatgctgat
    20401 gtacaaacga ctttaggaac ttatggtcat ttgtatctaa attcattgag agagataaca
    20461 aatgatccca cagattatat ccaaactaat ttttctaaaa tcaaaccgac acctatttac
    20521 gataataaaa aataaatgat aaacaaaagt aggatcacac caaaagaaac acactagaaa
    20581 aactataata caggcttttc tagcgtgttg tttcttattt ccgcctattt atacattata
    20641 ctcataaagc atttaatgca tcgatcgtag ccgcattgtc tatatgggta tataaagttg
    20701 ttgtattggt acttgaatgt cccaattgtt gggcagttaa taccaatgaa tttgtctgtt
    20761 ggtagagtct agtagctaat gtatgacgca gtttgtgagg gctgacttga attctaaagc
    20821 cttttgaata ttttgctacc atccgttcga ttgcaatacc ggatatacgc ttggcttctc
    20881 cttggtaaat tgctagaaat aaagcttttt cgttttctgt tactttatat cgttctttcc
    20941 gaatagctag ataattagct aaatattcca ttgcgaatgc tgctatattg acagcatctc
    21001 ttttatttcc ttttctagtt actaccacat tattcttatc taaagatagg tcatctaaat
    21061 taatatttat aacctcggag agtctcaatc cagatcctaa aatcagtgca ttgatcgcca
    21121 ggtctctttc tacatctcgt tgataataga tcaaagctcg tttactcaaa gtatttttat
    21181 attcattggc aatataatct agataatttt gtgtatcctt atctagaaat aattttttct
    21241 caatggcatt ggcacgtgca gctaacgtag ttttatctgt gatggttgct acttgatcca
    21301 taggattatt gtctaagtat ggttttccta gaggattatt ggaacttttc gaaagatatt
    21361 taaatagaac ctttaatgcc gtgatatttc tattgatagt agaagtagat agacctaagt
    21421 tattattatc tttgagctta gtttttgttg tctctttttt tacattttct tttgtgcgac
    21481 gtttcaaatg agatttgtac aagtttaggt cataggcagt taactgtctt atcgaggtga
    21541 tagaagtatc aggaacgata gactcactca gaagccattc ataaaagagc ttataatttt
    21601 ttagatattc taaagtggtt cgaggtgatt gatctttatc ctcacgatct tgaacaaaat
    21661 cttttatata atcgggcata tgaagtatga ttgtatctat tttttgatat atttctgtat
    21721 ttttcatgtc attcatcctt tcaataagta ggggagaaga gtatctctat aattggacta
    21781 taccaattat agaatgtttt gtcaattggt atgctctaaa atttattaat ttgtttcaaa
    21841 aaaagcatat aaacaggtat acgtttaata atttaaaata aatggtatac ataaataggg
    21901 aaggaaatag aagggagcaa tctaaatttt ttgaattttc ttaattaatt atatagaaag
    21961 aacaatcttt ctatagagtt agacatgaga gggactatgc ttgatttaaa atcaacaagc
    22021 gtatatactg tatatacaaa gaaaaaggag ggagaagact atggcaatta aagaaagaaa
    22081 atttagggaa gtggggaact cagtagtatt aactatttca aaggagtttc ttaaagaaaa
    22141 tgggttaaat cccggagata cggttttttt agatgaagaa aaacttcgag cggcaattac
    22201 aaaaaaacaa gccttatctg atgaaattga tttagcaata tctcaagcat taactgaata
    22261 tgatactact tttagagagc ttgtcgataa atgagtattc attacctaga agccaaagat
    22321 attataaaaa tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc ccttggtgta
    22381 aaggatgcga atgctttaga aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa
    22441 gaggtttatc cttcgattta tgaaaaagca gctatcctac ttatacaatt gataaagaaa
    22501 catccttttc acaatgcaaa taaacgtact gctttcttag ctacttttgt ctttttgaaa
    22561 atcaatggac agcttttagc tattgatcaa aaagatgctg taaatttagc ggtatatatt
    22621 gctacgtatg ataaggattt cgatcaacta aaatatgaag tgatcaacac tataaagaca
    22681 catacgcagc ccatttaagg agttactgta tgtgtcttta tagggttatt agctgctata
    22741 gcgaaataat tgaagtaaat tacaaatagc tataattcaa atccaactat atagaaaacg
    22801 taaattttct atatagttgg attaagataa cgactattta ttttatcata tttttactat
    22861 accctatact tttacttggt atatttatga ataatcgcaa atttacccct atactactag
    22921 tttgtaagtt agattaataa aaatactaaa gttatttgtt cttttattaa atacacctac
    22981 aacttataaa ttgatggtat ttagataaca cgaaagaata aaaataacag ctgatagtaa
    23041 agtttgttga cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga
    23101 aaacttatga aatgaaagac cttttacatc ttgcagaaat cgttactggg attaaactaa
    23161 gcaataaaaa ctaatgttcc cctcattctt tgaacttcta ctatttgatt tatttattct
    23221 gataaatgac gatttcacct tcaggatctt caaatcccgt atagaattta tttaagatct
    23281 cttcagtatc agtaatagga taggcgatag gccacatacc ctttgatggt tagaataaac
    23341 tgctcaaaat ctgtatcgta tgttggcttt agcatattaa caaaggctat ctccaaatta
    23401 taaaccataa gcgttgaatc gttcttaaca atttcaattc ttcctaaccc aaagtcagaa
    23461 gatataatta ccggacttgc cttacccttc atgtgggttg ttgagattcc atatttaaag
    23521 ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa atctttcccc
    23581 tgctagtctc ctaagtccct ttgtagcgga tatggcacca taatctcatt ttatcgaagt
    23641 gagtcatggt ttatctgttg gccaaccaaa attgatacaa tctaacgatg caccctctac
    23701 ggcattctag gggagtgcac ttgcattcac ccaataaaaa ccggcagtta gcaaagaact
    23761 tatgtagtcg tatgttctta ctgttaacat acaagaggtc tcctttccac aattgcttag
    23821 cagtaaacgg gaatttacaa agctaatctc ttccattttc ttatctttgt tctaaaagtc
    23881 ccaaatggag caactgtatt tacatgaata aatttatata cttcccatac agccgttttg
    23941 gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata gatcttcatc actcattgaa
    24001 tcaatcatca tgttaatatc atcaatatta cgattgagaa tttcctctaa ttctttcaat
    24061 ggtagatgtg cataggtatt tgtaaaccac ttatataact ctcctaactg attccattta
    24121 aattgatcag aaggcgtttt aacagataat ccatttcttt catcattttc ccattttaga
    24181 attaaattcg tccaaccaac ttgatatgct aaattttcag caggtgttct atccacttcc
    24241 tcaactcttt tatccttcaa ttcttcagga atagtttcaa attctaaaat atatttagta
    24301 taagtcctat ttatttcttt ttttaattct tctttatttt tatatgttct cattatatta
    24361 cctccataaa ttcctaattg tctcctttaa ttcatctata atttcatcca cgatatttta
    24421 atccgttcta caatgcatat ctgatatgca ttgactgtct aactcatatg tgaatgattt
    24481 ataaaatatc ccaacgtctc atctaaagaa atgttgatcg tcagcaagga gctaaaaaat
    24541 acgaatatat ttcttttgta gtaaaatcgg actggtttgc atatcatttt cttcgtaaat
    24601 atcaaaatca tgttaactac tatcaattac taataaaaac taccacttta agcgttaaga
    24661 actgttaagt ttagttaagt gaggatagtt cacaaaatcc attgttaaac ttcacgaaag
    24721 tggtaaaact caaaatgctt tatataggat tacagcatcg ctctttcttc gattgcgcgt
    24781 tgggtaaaaa atattatgaa gtaaaaattg acgatgacac gttccttacg gctcatcaga
    24841 tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat
    24901 tgccatattc acgcctcgct cgacgaacta ttaaaggtca agatttacct gaagatcatc
    24961 ctttttagga aattgaagct ttttatgttg cacaatgcat ttataatact acaatgatgt
    25021 tttcaccaga tattcttcta gggtgcggtg taatgaaaca gatacacttg caaaagaaaa
    25081 ttcaccgcga atttactaaa attatgaata attatgttaa tcttccgaaa tttttttcca
    25141 aaattaaggg atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa ataacttctt
    25201 tttaaacaat tatagaaaac cgatactctg ttaagttggt tgacacggtg tcgattttta
    25261 tgactttctt attctgaaac ttcactaacg gacagtacct aaaaagtcta tagtatggac
    25321 gtttacgagt actattttat caaaaatgct gctcatttca acttatatgt tttagtgaac
    25381 tacccccact tagcttggat aggctacaat taactagaca gaaaaattaa ggtgtgtaga
    25441 ctagaagaaa acataccagg aggaattttt atgtctaaga gaacacgaag aactttttca
    25501 caagaattca agcaacaaat cgtcaatctt tacttagctg gaaagccacg tgtagaaatc
    25561 attcgagaat atgaactaac
//
LOCUS       DAYYHS010000038        24447 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000038 DAYYHS010000000
VERSION     DAYYHS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.038"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..837)
                     /locus_tag="ACHA6A_001512"
     CDS             complement(<1..837)
                     /locus_tag="ACHA6A_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8969710.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFM"
     gene            complement(1061..3001)
                     /gene="thrS"
                     /locus_tag="ACHA6A_001513"
     CDS             complement(1061..3001)
                     /gene="thrS"
                     /locus_tag="ACHA6A_001513"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8969711.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3338..3517)
                     /locus_tag="ACHA6A_001514"
     CDS             complement(3338..3517)
                     /locus_tag="ACHA6A_001514"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8969712.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3564..5387)
                     /locus_tag="ACHA6A_001515"
     CDS             complement(3564..5387)
                     /locus_tag="ACHA6A_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8969713.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5665..7944
                     /gene="helD"
                     /locus_tag="ACHA6A_001516"
     CDS             5665..7944
                     /gene="helD"
                     /locus_tag="ACHA6A_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8969714.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8037..8684)
                     /locus_tag="ACHA6A_001517"
     CDS             complement(8037..8684)
                     /locus_tag="ACHA6A_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969715.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8863..9582)
                     /locus_tag="ACHA6A_001518"
     CDS             complement(8863..9582)
                     /locus_tag="ACHA6A_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8969716.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9713..10864)
                     /locus_tag="ACHA6A_001519"
     CDS             complement(9713..10864)
                     /locus_tag="ACHA6A_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8969717.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11081..11821)
                     /locus_tag="ACHA6A_001520"
     CDS             complement(11081..11821)
                     /locus_tag="ACHA6A_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8969718.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11825..12148)
                     /gene="rsfS"
                     /locus_tag="ACHA6A_001521"
     CDS             complement(11825..12148)
                     /gene="rsfS"
                     /locus_tag="ACHA6A_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8969719.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12205..12798)
                     /gene="yqeK"
                     /locus_tag="ACHA6A_001522"
     CDS             complement(12205..12798)
                     /gene="yqeK"
                     /locus_tag="ACHA6A_001522"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8969720.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12782..13432)
                     /locus_tag="ACHA6A_001523"
     CDS             complement(12782..13432)
                     /locus_tag="ACHA6A_001523"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8969721.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13442..13753)
                     /gene="yhbY"
                     /locus_tag="ACHA6A_001524"
     CDS             complement(13442..13753)
                     /gene="yhbY"
                     /locus_tag="ACHA6A_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8969722.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13769..14878)
                     /gene="yqeH"
                     /locus_tag="ACHA6A_001525"
     CDS             complement(13769..14878)
                     /gene="yqeH"
                     /locus_tag="ACHA6A_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8969723.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14875..15402)
                     /locus_tag="ACHA6A_001526"
     CDS             complement(14875..15402)
                     /locus_tag="ACHA6A_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8969724.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15582..16367)
                     /locus_tag="ACHA6A_001527"
     CDS             complement(15582..16367)
                     /locus_tag="ACHA6A_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8969725.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16360..17229)
                     /gene="accD"
                     /locus_tag="ACHA6A_001528"
     CDS             complement(16360..17229)
                     /gene="accD"
                     /locus_tag="ACHA6A_001528"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8969726.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17230..18609)
                     /locus_tag="ACHA6A_001529"
     CDS             complement(17230..18609)
                     /locus_tag="ACHA6A_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8969727.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18617..19036)
                     /gene="fabZ"
                     /locus_tag="ACHA6A_001530"
     CDS             complement(18617..19036)
                     /gene="fabZ"
                     /locus_tag="ACHA6A_001530"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8969728.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19061..19537)
                     /gene="accB"
                     /locus_tag="ACHA6A_001531"
     CDS             complement(19061..19537)
                     /gene="accB"
                     /locus_tag="ACHA6A_001531"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8969729.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19540..20775)
                     /gene="fabF"
                     /locus_tag="ACHA6A_001532"
     CDS             complement(19540..20775)
                     /gene="fabF"
                     /locus_tag="ACHA6A_001532"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8969730.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20794..21531)
                     /gene="fabG"
                     /locus_tag="ACHA6A_001533"
     CDS             complement(20794..21531)
                     /gene="fabG"
                     /locus_tag="ACHA6A_001533"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8969731.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21543..22460)
                     /gene="fabD"
                     /locus_tag="ACHA6A_001534"
     CDS             complement(21543..22460)
                     /gene="fabD"
                     /locus_tag="ACHA6A_001534"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8969732.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22567..22791)
                     /locus_tag="ACHA6A_001535"
     CDS             complement(22567..22791)
                     /locus_tag="ACHA6A_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8969733.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22844..23806)
                     /locus_tag="ACHA6A_001536"
     CDS             complement(22844..23806)
                     /locus_tag="ACHA6A_001536"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8969734.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23816..24256)
                     /locus_tag="ACHA6A_001537"
     CDS             complement(23816..24256)
                     /locus_tag="ACHA6A_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8969735.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6693 a         5442 c         4249 g         8063 t
ORIGIN      
        1 cataaaatat tcttggattg cttctcgtgt ttttttcact ttttcgctaa gcttttcgtt
       61 tttagccata tagaaataaa aaatgatatc gatcgtagca aattgggcca aaagtgaatt
      121 agttgccgca cttcgattcg tgatttcttt tggtctagat gtttgtacga gtacatcggc
      181 tatcttgcta agcgggttat ttccaaattg cgtcaacgca atgatttcga tattcatgct
      241 tttcgccagc tcggctaatg cgactacatc tgcacttcta cctgagtttg aaacaagcca
      301 aagcactttc tttttctcat tgctgacaag ttgcgggagc aagacgtgga tatctttttc
      361 aaaaatgatt ggcttaccga cacgagacca tttttgcaga atatcttcta ctactaaaga
      421 agaggcgcca accccatata aaaaaacagt ctctgccgat tccaaacact ctacgacttt
      481 ttggatcgtt tcttcttcta gaatatctac tgtttctttt atcgctactt gtgcattgtt
      541 accaagtttg tttttgatag agtaaaagga ctcatttgct tctatatcac taaaaccttc
      601 atcgattcca gtctgtagtt ctgttgaaat cgtgattttg aagtccgtca gactttgaaa
      661 accgattttt ttggaaaaac gtacaactgt cggcgcactc aacccggaag cttctgctaa
      721 ttcattagct gtcattgtag ggaccttctc aatattaccg ataatgtagt tggccaactt
      781 tttttctgct gaagaataat cttccatcat actttcaata cgactaatta cgctcataag
      841 ttcctccacc cttttaatga tagtctatgt aagtatacca tatcagaccc ttattcacgg
      901 tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac agaatatctt atcccatcaa
      961 tcccacagat tctattgagc atctgtgtct ttgaggaata cctgttatct attgttcgga
     1021 ttgctttcta tctactactt ttttacagga aatttaccta ttagttttct ctgctgtaat
     1081 ttttgacttc tgcttccata gcatcaataa agatattcaa atcttctaca gctgtttctt
     1141 tacttccata acgacgtacg ttcactgttg catcttccaa ttccttgtct ccaacaacta
     1201 attggtaagg gatcttttgt gtttgagagg cacgtatctt atagcccatt ttttcattac
     1261 gatcatcaac ttctacacgc aaccccttca tttgcaaacg ttctttgatt tcataagcat
     1321 aatcgccatg tgcatcaaca gaaacaggga tgattgttgc ttgtataggt gccaaccatg
     1381 ttggaaaagc gcctttgtat acttctgtta aataagcgac aaaacgttcc attgtagaaa
     1441 caattccgcg gtggataact actggacgat gcgtattttc cccgtcttct ccaacatatg
     1501 tcaaatcaaa gcgctctggc aataagaagt ctaattgtat agttgataat gtttcttctg
     1561 taccaagagc tgttttgact tgaacatcta gttttggtcc gtaaaaggct gcctcgcctt
     1621 ctgcttcaaa gtaatctagt tctaattcat ccatagcggc tttcagcatt gcttgggctt
     1681 tttcccacat tgcgtcatca tcaaaatatt tatctgtatt gtttggatcg cgatagctca
     1741 agcggaaacg ataatctgta atattgaagt cagcatatac tgccaccatc aattcaagcg
     1801 tacgtttaaa ttcatctttg atttgatcag gacgaacaaa tgtatgacca tcattcaacg
     1861 tcatttctcg tacacgttgt aaacctgaca aagcacctga tttttcatag cgatgcatca
     1921 ttcctaattc agcgatacga attggtaatt cacgataaga atggatcgtg tttttgtaga
     1981 ccatcatgtg atgagggcag ttcattgggc gaagaacaag catttcgcca tctcccatat
     2041 ccattggcgg gaacatgtct tcttgataat gatcccagtg tccagatgtt ttgtacaact
     2101 caacatctcc cattattggt gtatatacat gttggtaacc tagactaact tctttgtcta
     2161 cgatataacg ttcgatcgtg cgtcggattg ttgccccttt tggaagccag aatggcaaac
     2221 cagaacctac ttctggggaa accatgaaca agtcaagttc ttttcctagt ttacgatggt
     2281 ctctttcttt tgcttcttca cggagacgga tatattcttt caaagctttc ttgtcaaaaa
     2341 aggctgtccc gtatacacgt tgcatcattt gattgtttga gtttccacgc caataagcac
     2401 ctgccactga taataattta aatacttgta tacggccagt ggatggaaca tgtggtccac
     2461 gacacaaatc gatgaattcg ccttgttgat aagcagtaat cacttcttct tctggcaatt
     2521 cactaatcag ttctactttg tacggatcac ttgcaaaaat ttctaatgct tcctgttttg
     2581 aaagaacacg gcgttcgatc ggcagatttt ctttgacgat tttcatcatt tctgcttcga
     2641 ttgccggcaa atcttctgca gttacttggt tttgtccatt atctgtatcg tagtaaaacc
     2701 ctgaatcgat agctggtcca acgccaaagt ggatatttgg atagaggcga cgcatggctt
     2761 gggccatcaa atgagctgct gaatgacgga ctaagccaag tgcatcttca tggtctggtg
     2821 tgatgatctc aatacttgca tcttcctcga ttggacgagt caaatcaacg agttttccgt
     2881 tcactttacc agctaaagct ttttttgcta agctttttga aatgctttca gcaattgcca
     2941 atgttgttgt tccagactca aattctttga ctgcaccatc tggaaaagta atttttatca
     3001 taatagtttc ctccttgaaa tgttttgatt gatcagatca tcaagctgaa aaacaaaaaa
     3061 atcctagcat ctcttgaaaa gagatactag gacgaaaagt tcgtggttcc acctaatgtt
     3121 caaacgcaaa tgcgttcctc aagcgttgta acggtccgac cgcccttgct tcaacaaggg
     3181 tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt ttcagcctag cttttcctct
     3241 cttatcgaag actctcaaat tcagttggct ttctcatcaa tcaattatgt gtttatttaa
     3301 ccactaataa atagaaaaat caagtgggaa actaaactta ttttttccat tccccattta
     3361 caccgtatgt tccttttttc atctcattga tgatgacatg gatgtgttct tttggtgcct
     3421 gcgtgttttt atgaacagct tccgtgatat ctttcatcat attttctaat tgttccggac
     3481 tgcgaccttc tactaattcg acatgtacga atggcataat gctctctcct ctttctgatc
     3541 gttctttttt caactcgatt ctatcatact atgtttgtct tgggtactga atcaggaaaa
     3601 atggctggat ctttttttgc tctcaaggca ctcaaaatag agaccgtatc cacgacttct
     3661 tgcaaaacag ctccgaataa tgcggggatt agtcccgtac ttgcaattaa catcaaaaaa
     3721 atacaaataa agatgccaat cagtacagac tgtttcgcta ttctcatcgt atctcttgaa
     3781 atcactactg cttttgctac acgcaagaga tcatctttca agatgaccgc atctgcactt
     3841 tcactagcag ctgtagaacc atgagccccc attgcaatac cgatatctgc agcagcaagt
     3901 gctggtgcgt cattcacgcc gtctcctacc atcgcaacag gtctttgatc tttagggata
     3961 ctttctaaaa tggtcagttt atcttgaggc aagctacggg catgtacaga atctaattgg
     4021 actttttctg caatcgtttg agcaatggca gatccatccc cggtgatcat catcaaattt
     4081 ttcacatcaa gttttcttag ctccttgatt gtctttgctg cttctgggcg aatctgatcc
     4141 gaaaatagga tcgttcctgc aaaagatcga tcaattgtta catagattgc tgtattggac
     4201 gtttctattt cttcagctga aacaaaagag gcacgtccaa tctttacttc atgattattg
     4261 actactgctt ggattccttg accggtcact tctttcagtt gactaacagg atacagttgg
     4321 ctgtttttca cgttgttcac taacgaacga gctaagatat ggctagattc ctgttctgca
     4381 ctagctgcat acagaagtag ctcttctttt tttattccat tgactggctg aatcgcctct
     4441 acttccaata tccctttggt aagcgttcct gttttatcaa aagcaatcgt ttttgtacgt
     4501 gcgagctttt caatcgttgt tccagttttt acaacgatcc cgtttttact tgaacggctc
     4561 attcctgcaa ctaaagcaat gggcgcagct agaatcagtg gacaaggtga tgccactacc
     4621 agtacctcag caaatcgtaa agggtctttt gagaaccacc aagaaatacc accgatcaaa
     4681 taagcaatca acgtgaaagg gactgcataa cgatcagcta aacggacaaa tcgggcaggc
     4741 ttttcttttg attcttcgac tagtttaata atcatttgat actggctgtc tgcggcacgt
     4801 ttttcagcga tcatttggat cgatccgtct ccatttaaag accctgacat aatctgatct
     4861 ccaggtttct tggtttccgg gcgggactca cctgtcaaag aagattcatc gaccgaagat
     4921 tctcctttgg tcactcgacc atctgcgggt acaagttcgc caggcttgac tagtaactgg
     4981 tcatcaatat ttatttcatc gattactata tcctgcaatt ttccttcttt gaatcgatgg
     5041 gcaattcttg gggtgttatc taacaaagac tgcagttctt tacccgctcg tttattcgcg
     5101 taatcctcta gactatctcc gcccgtaagc atgatcaata tcatcaaact agcccagtat
     5161 tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta atacatctac gccatatctt
     5221 cctgaacgta acgtcttgat catttccaaa aacatcaaga aggcgagaat ccctccagta
     5281 aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa caaattcact taataaagct
     5341 atcccaccaa taagcaaagt aagtaaaaat tttttgaaat gtgtcattgt tttttctcct
     5401 cttttatgtg acttagagag gttagtaata aatttccagc tgcctctttc atcttaagca
     5461 tagtccttga ctagcacttt ttaaaacaat aggccttaat tgaaaacaac tatcactatc
     5521 agcgtttcca aaattcatca gttctacaaa caaaaatatt cagcaaggat tactttttcg
     5581 attcatcagt taaaaagtac cttttttcga aaaactatgc tatactttta attcgttatt
     5641 tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac tagaacaaac gcacgtggat
     5701 gaaacgatcg aactaatcca gatcgaacaa aatatgctgg atacaaaaca acaagaattg
     5761 actcaggaaa tgaatcattc gacaaaagac acagctaatc acaagattcg agctggttcc
     5821 aatgaagctt tttatgaatc tgctgttgaa tatcgccaac atgagcagga attgcttttg
     5881 aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa cgttgtctgt catgaaagat
     5941 agtccttatt ttgcccgtat cgactttaaa gaagaccgtg aaacagaaac attatatcta
     6001 ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg ttattgactg gcgcgcacca
     6061 attgccaatc tttattatga aggtgagatt ggtcccgcat tttatgaaac tgaaacagat
     6121 agaatagatg tcgatctgca gttgaaacga caatttaaaa tccaagaagg acgtatttta
     6181 tctatggtag atacgtcgga agtaatcaat gatgactttt tgctagagat attggatgaa
     6241 tcatcaagca accatatgaa aaacatcgtc tctacgatac aaaaagcaca aaatgcaatc
     6301 attcgggaca caacaagtaa agtaatgctg attgaaggaa tcgccggaag tggaaagaca
     6361 tccgctttgc ttcaacgtat tgcttttctg ttgtatcata atcgcaagtg gctcgaggct
     6421 gaaaacgttc ttctattttc acccaaccat ttgttttctg attacatctc gactgtattg
     6481 ccttcattag gtgaaagcgg cgtgcctaca cagacattca aaaattattt ggtgcagctg
     6541 ctgccagagt tttctttact agatgaacag caacaagaaa gtggtttttt gctaggggaa
     6601 aaagatccga tacaagtgat gaaaagcgga ttgaccttag tcgatcagat tgatcgctat
     6661 atccaaggca tcacaaacta cggtcccttg ttccgtgata tgaaaataaa tggccgtacg
     6721 atcctatcaa aggaatcgat tcgccaatgg tataaagaaa caaatgaaca attaccttta
     6781 catcatagac tttctctatt acaaacgaag ctcctgaaaa aattaggcgg tctgcaaaaa
     6841 gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag aacagctcca agaactatac
     6901 gcaacagatc caaatcttga atatacagaa caaaaggagc gttcattacg taaaaaactg
     6961 gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc gagggatcat gaattatcaa
     7021 tttgtcaata taacgaaaca atatctccac tttctacaaa ctgtgccact ttctcttcta
     7081 acacgacata aaatcagcga gaccaggtgg caggaacatt tgacaactgt tcgtacacac
     7141 ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat tctttttact gatgcgccga
     7201 acatatccag tgacggttac acacaaagct cgctatatct ttattgatga aatgcaggat
     7261 ttcgcaccat cacaagtggc attgctgcaa gccttgttcc caaaagcaaa tttgactttc
     7321 tgtggagatc taaatcaaaa tgtctttgga aatgaaacga tcgttggatc attggaagac
     7381 cttttcgtgg accgtgaagt cactcgtttc caattaacga caagctatcg ctccacaaaa
     7441 gaaatcactg attttgctaa tcattttatt gtgaaagaac atcaagtaga aacaacagca
     7501 agaaaaggag accttccttt gctcatccaa ggaacaacag atgatatcaa attgaattgg
     7561 ttagcagata ccattcgaac gactcaggga aaagcccggt attggcgtac agcaatcata
     7621 gctaaaacac aagaagaatg cgaagctctt tatgctcaac tgccagacga gttgaaacaa
     7681 caagtccagc tgattttaaa tgagagtgac tttatgaagc gttccattat tttacttcca
     7741 gcctttttag caaaaggtct ggagtttgac cgagttttct tgtgggatgt agatgcagaa
     7801 catttttctt ctcagcaaga caaattgatc ctttacacca tgtgtacacg tgcgatgcat
     7861 gaattgaccc tttttacctc caataatgaa agtcctttgt taaacaatat ggatatttca
     7921 tcttatgaga aaaaagtact gtaagtaaaa agacaactgc tatcattaat gttagcagtt
     7981 gtctttttat ttacaataat aattgcagaa aaagtaattc aatcatttaa ggtcatttat
     8041 tctatatata aatccaaatc tgtttcagga tattttaatt tgacaagcga aataatcatt
     8101 ttcagttctt ccatgttttc tggatattct gggtcaaata ccttgctatg acggccaatc
     8161 ccgccattgt cgatcaatcg gtaaattcga ttccattcaa tgaattggtc aattgattgg
     8221 aaattgctga ttggatagcc gccacaatcc aaaatctctg atacacgaaa tgctaggtat
     8281 gcactatcga tcactttatt ttttttcgtg tctatcttct catttctttc gataagaatt
     8341 ttttcaggac gatgtttcaa gctccactct aatagtagaa agaaagcctc catccgatac
     8401 tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa aaagcagcgg aatcagataa
     8461 ttttgtctat aaatcagcat tggtgcgcgg tccaccatac actctaaata acggttcaga
     8521 taccctgtac cgacatagtt tcctaatcga tagccttcaa cgccgaatct tgcgtatttt
     8581 tgcgctaatt ttgaacaatc agttacgttc acagctaact cttctacttt gcggtaaccg
     8641 cttaagatac gttcaaaagg ttctaaagtg atttttgctt ccatattctt ccccgtccat
     8701 cttccacata cttcatatca tttcacatga tacttatgct ctcatcatac cacaggttga
     8761 attagaaaag aaaataaatc acacaaaaaa gcaagacaat ctgttagtga gattgtcttg
     8821 cttttcgaaa aagactgaat acttttcgtt aaaactggtt gcttagtctt ctaatgccac
     8881 attatggaac acatgctgta catcttctag atcttccagt gcatcaatca ttttttcaaa
     8941 tgtttcttga tcttctggag atagtgttac ctcgttttga gggatcattt ggatctcagc
     9001 gattgaaaaa tcagtgattc ctttttcttt taatgcttct tgtatactat ggaaatccgc
     9061 tacttcaccg taaacgatga tttgtccttc ttcttccatt acatcgcgaa catcgatgtc
     9121 tttttccatt aggtattcaa ggatctcgtc agcgtcttct cctgcaaaag caaagattgc
     9181 tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc atgttcccgc catttttccc
     9241 aaatgctgcc cgtacgtctg ctgctgttcg attcacattg ttcgtcaatg tatcaacaat
     9301 caccattgag ccgcttggtc caaatccttc atagcgcaat tcagagtatg tttcatctcc
     9361 tgcacctttt gctttttcga ttgctcggtc aataatatgt tttggtacat tataagtttt
     9421 agcgcgatca atgacgaaac gtagtttttg gtttgcatgc ggatcaggat cacctgattt
     9481 tgctgccgcg tagatttcaa tcccaaattt tgcataaatc cggctattat tagcatcttt
     9541 agcagttttc tttgctacga tatttgccca tttacgtccc atattttcac ttcgctttct
     9601 gatttgatag tttcataacg caaaaacgtc ctgttccatt atacttgtga atcacgattc
     9661 tgtgaagctt tttttctttc ttccttcatt cttctcattt ttagtcaatt atctatactc
     9721 ggattgggat ttgcccgaaa ttctgttccg gaatattgcc tcccaattga tagatttgat
     9781 cacttctaac taacagttcc gcttcttgtg cctctttttt tccgattcgg gaataaatag
     9841 aaatctcttt tttttctttt aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa
     9901 gataattttt attttgttgg acaggaatct cttgcttttt gatattcagc aagacataac
     9961 aagataatcg ttggatccgt gtccatgtat atctttttgt ttttaccttt gacacaaaat
    10021 cctcaaaact atcagcagtt gcagctgctg ttttcatgcg cgattctatt ccttcggtca
    10081 tctgatagat atctgtcagt tccagcaaag tagaagataa caaccgataa cgcaaatacg
    10141 gccaataaga ttcccaagtc tgcagcgaac aagttgaaag atcttcttct gtctgaggcg
    10201 gcacaagtcc agctattctt tttccttctt ctaacgcatg gcgaatggct gttgcactgg
    10261 ctatcctctg atctgtgatg attttttcat gatagcctgc acttttacgt cggatcggat
    10321 gtaattccat agggtggaca tacttggcat tttcctttgc ataacttaat cccaagatat
    10381 gatttggcga agaaaaatca agcgtaatat cgggatacag actgcgaaaa acctcagtca
    10441 ttttttgcgg ataactatac gtttgatcag acagttcttg aaatgcctct tctatcctat
    10501 cttgattctc ttggacaaag cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg
    10561 ttccaaaaca caaactgtca cagtccagtg tttgcaagat ttttactgca cctttagcaa
    10621 aatagtcagc tgactgaacc gcccattcga cgggaagctc cactactaaa tccacaccat
    10681 tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat agccggttcg cctctttgca
    10741 aaaaattccc gctcattaca gcaatgacga cctctgctcc tgttattttt cgtgccattt
    10801 ccacatgata gcgatgtcca ttgtgaaaag gattgtactc tacgataatc ccgcagcttt
    10861 tcatgcgcat cctccttcat agttatcgat aaaaaagaaa tagagacaag taaaaaaagg
    10921 caaaaatcgg aaaaaatacc cgaactatat aaactattct cgcaaacaca atacttctgc
    10981 gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc ggatatataa tttatgttga
    11041 agatttattc ttttcttgtt aactcgagca ggcaaaaccg ctagtctttg tatgctacaa
    11101 agaaccagcg ctttgatttc tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga
    11161 ctttcgaaaa gccgctgttt tccagcattc ttaaataact ttccaatgga taggtacgct
    11221 cttgatgtag ttcatcttca cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa
    11281 aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag
    11341 caaaattatc cccttgataa tgatagctat attctggaaa aacctcatct acttgatagg
    11401 tggagtgtac gtcaaataaa aatcggccat tttcttctaa cgcttgatag acttcatcaa
    11461 acacttgctg tacctcttgt ctatttttca tatagcacag cgaatcagaa aaacaggtaa
    11521 ttgcctgata ttgtccgatt tctgataaat cgagcatatt gccttccaca aattggatcg
    11581 atgcgtcttc ttcatatgct cgctggctgg caatcatcaa catctcttct gaaaggtcta
    11641 accctgtcac tttatatcca tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg
    11701 ccagttccaa aatatttttt gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt
    11761 gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa agcaaatgtt tcataagcca
    11821 tatgttaatc caaccattca ctaagatcga ctagcggagc atctgaccat agtttttcca
    11881 gattatagaa atttcgttct ggcgcatgga aaacatgtac gatcacatct ccaagatcga
    11941 tcaataccca ttttccgcct tcttttcctt caatacgttt tacttcatat ttgttttctt
    12001 cttctttatc aatgatttct tctgtaattg cgttgatttg acgttcgcta tttgctgaac
    12061 agatcatgaa ataatctgcc aacaaggaaa catttcgaac gtctaaagcg ataatgtctt
    12121 ctgcccgttt tgaatctgct gcttcaatcg caatttttaa ttgttcttta ctatctatga
    12181 tgatttcctc ctaatatgag cagcttattt ttttgctacc cagtaattat atgtttcgat
    12241 tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc
    12301 aaatgctaca gcttcgtcta gatcagccca tgcaatggct cgtgcatctt gtaccccagg
    12361 aaaatctcga cctggttcaa tatagtctgc tacataaatg attttgtcaa ggagactcat
    12421 cttagcagcc ccagtcgtat gcacccggat tgcatgaaga atctctgcat cggtgattcc
    12481 taattcccgc tctacaaacg aagcgccaac aagaccatgc cagattgcat tattataatt
    12541 caataactcc ggatcaaaac cgtctcttac gattaccatt ttgaactcct catctggtcg
    12601 ttcttttgca taatcatgtg tcaatgcagc aatactagct ttttcaggag aagcaccgta
    12661 ttttttggca agagccacgg ctgtttcttc tactccaagc acatgcttga agcgttgttc
    12721 gctcattctc atctgtattt tttgcatcag gttttctcta gaataagcag tatattcatt
    12781 actatagtcc atattcatac aacccttcat tttgaatata atcaattact ttatctggaa
    12841 caaggtagcg gatagaacag ccttctttga tcttttgacg tattttcgtg gaactgatgt
    12901 cgatctcagg tacatctacc caaattacag gatatggagt atccgttgtg tatcctggtc
    12961 gacgtatacc cacaaagttg accatagaag tcagctcatc gattttatac cattttggca
    13021 aatattctac catatcccca ccgataataa agtaataatc tgtatccgga ttgttttgcg
    13081 tcaattcctt catagtatca taggtgtaac ttttcccacc acgagctaat tcaatcgttt
    13141 caatctgcaa aaaaggattg tcttccaccg ctaattccaa catgttgagt cgatgttttg
    13201 catcaatcgt ctgtttctca tcaacatgag gtggctgata agaaggcatc aaaaatacgc
    13261 ggtcaagacc taaattccgg ccagcttgtt cagccatcac taaatgtgca aggtgaacgg
    13321 gattgaatgt tccgcccaag atccctactt gctttttctt ctctaagaat tgaggcattt
    13381 cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg cgtattcatc atggtcactc
    13441 cttatattgc ttttacttct tttgatagcc tttgatactt ttcttttgtg gaaggtttgt
    13501 ataatacaat aacacgtcca atgatttgga cgatttcgca atctatttct ttttctaata
    13561 cttgagctgc ttcttctgca atctcatctg tattttgaag taacgatact ttgatcagtt
    13621 ctcttttttc taaagcttct tcaatttgaa cgatcattgc tgaattgatc ccaccttttc
    13681 cgatttgaaa aatcggctga agatgatgcg ctttgctacg taaaaaacgc ttttgttttc
    13741 ctcttaaact cataattttc cttcttcctt aaatcaatgc tttgcgtttc aatacatcta
    13801 caccctttgg cgcccagccc gcaattacta ctggcgctgt tacagtgatc cagcctagac
    13861 cggcaaaaac gatgtctgtt ttttctttca cagaaaactc aaaacgaaca agctccggaa
    13921 atgcctcgac ttcatcagga cgaggaggct gtaataatcc acctacatgt ttttgataaa
    13981 attcgtctgc tttttccagt ttcgttcgat gaatatttac atcatttgac acataagcaa
    14041 taaatgaagc acgattgccg ctgataaaat caaatcttgc aagtccgccc aagaaaagtg
    14101 tttgcccttc attcagctga taaactttag gtttgatttc ttttaacggt gcaatgatcc
    14161 gcaaatcttt tttacctaag tagtgcgcca tttgatggcg atggataatt cccggtgtat
    14221 caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt atccaaggtc gttcctggaa
    14281 actgcgaagt tgtgattaca tcttgtactc ccgctgtatt tttgatgatt tgattgataa
    14341 gcgtcgattt tccgacattt gttactccga cgatatagac atcccgtcca tcgcggtatt
    14401 cttcgatttt ttctaatagt tccgtcatct catgagattt cttagcacta gttaataata
    14461 catctactgg acgaattcct gcttcgtaag cacgttcctt catccattgt gttaatttag
    14521 tttttttcaa tgatttaggt aatatatcca ctttgttccc aactaaaagt actggattgt
    14581 caccgacaaa acggtgtagt cctgggatta gtgaaccatt gaagtcaaaa atatccacca
    14641 cattgacgat caatgcatct ttttgcccta attcattcag taagcgcaaa aagtcgtcat
    14701 ccgtcaatga gacatcttgg gtctcattat aatggcgtaa tctgaaacaa cgctgacaat
    14761 agacttcccc ggaagctaaa cctttttcca aagccgtttg cggcgtgtat cctaattcac
    14821 cgggtttgtt tgtttgtata acggcaccac agccgataca ttgtaattct tctgtcattc
    14881 gattccacct cgccagccca tatcagggtg ttttgctaat agataggcca taattctttt
    14941 ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt gcgataatgg gttttacaag
    15001 gatgctacgt atccctgcac gattggctcc acgtatatca gtcataatct gatcgcctac
    15061 cattactatc tcatcatctg ctaaatccaa ttgtttcttt gctatgttga tccctcttgc
    15121 taaaggtttc attgctcgtg caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac
    15181 acgttttgaa ttattgtttg aaacaacaac aacagggatt cctgcatttt tcatttctaa
    15241 aatccaagta agcaactctt ctgttccatc tggattattc catgcaatca acgtattgtc
    15301 taaatcggtc aatactgctt tgatgcctag attctttaat tgggcaggtg taatctgata
    15361 gatggcttct accatccatg ttggtttgta atttgaaaac atgctgtctc ctcatctgca
    15421 aaaaatgaag gagcacataa agtactcctc aaccccgtat tatactgcat atcagcaaaa
    15481 aaaactacct ctatactcgt tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa
    15541 aataaagatt gatcacagaa aaatttagaa acaaaaaaca actaatattg gcggaacctc
    15601 ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa caagttccgt caatctttca
    15661 atcaacgctt ttttcataat gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac
    15721 gtttcaggaa taactcgatc aacgattcct aactcgcgta gttctggcgc agtaattttc
    15781 atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct
    15841 tctggagaaa gcacagcata aatcgtttct tccagcatcc agacttcatc cgctaaacct
    15901 agagcaaggg caccgccact tccaccttca ccaatgatga tggcgatgat cggtaccttt
    15961 aaatcagaca tttcgagcag atttctggca atagcctctc cttcgccgcg ctcttctgct
    16021 cctacaccgc aataagcacc tgctgtattg atcaaggtaa tcaccggtct attgaacttt
    16081 tccgcttgtt tcatcagtcg taaagctttt cgatagcctt caggatgggg agaaccaaaa
    16141 tttctgcgga tattttcctg cagatcccgt cctttttgaa taccgatcac tgttaccggt
    16201 ttgccatcta atcgagcgat tccaccaaca atcgcttcgt catctgcaaa taaacgatca
    16261 ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt
    16321 tctttagctc ttgctaactg aacgacatca tgtgctgtct tatccacgat ctatccatcc
    16381 tttctgtgta tgcaattcaa gaagcgtatg gattttctgt ttcaattcca tgcggggaac
    16441 aatctgatcg acaaaaccat gagataataa gaattcagcc ttttggaaat cttctggaag
    16501 ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca aaaccgatca agctttgggg
    16561 ttctgctaga ataatatcgc cttccattgc aaaactggca gttactcctc cggtcgtcgg
    16621 gtccgttaaa acagttagat aaaaaagacc ttcattgcta tgccgtttga cagcagcaga
    16681 gatttttgcc atctgcatta gagaaaagat tccttcttgc atccgtgcgc cacctgaagc
    16741 tgtgaacaag acgactggaa gactttcctt tgttgctcgt tcgaataatc tggtgatctt
    16801 ttctccgaca atcgttccca tacttcccat gataaaatta ggatccatca ctccaataat
    16861 gaagggaatc ccctgaatgg ttgctttacc agtgaggact gcttcatgaa gtcctgtttt
    16921 atcttgcatt tttttaattt tttccgtata ttcgggaaat gctagcggat ctttggttac
    16981 aagatccgta tcccattctt caaaactttt ttcatcaata atcaaagcta gacgctgcca
    17041 tgcaccgatt cgaaaagcgt atccgcaatg agggcatact ttttcttcac cgatgtcttt
    17101 cgtataaagg atatgtttgc aatttggaca ctttgcccac atgttatcag gaacagatgg
    17161 cgagttagca ggtttgctct ctctattagg attgatccga atatatttct ttttcttaaa
    17221 cagagccatc tatgcgcctc ctatctccgt ttctggtgtc caattcggta aaaattcttc
    17281 ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca ggatgactaa tcaaatccat
    17341 ctgaaattct gcgttcgtga tgattccttc tgtgataaat tcacttaagg ctcgctgcat
    17401 cttcattaat gcttcaaaac gcgtatttcc atggacgatt atttttgcaa tcattgaatc
    17461 ataatatggc gggatcgtat atcccgggta aactgcactg tctactcgca gtcccatccc
    17521 acctgctgga agcagtaagt tttggatctt ccctggagaa ggagcaaaat ggaaagcagg
    17581 attttctgca ttaatccggc attcgattgc gtgtccttgg aatactactt cttcttgaca
    17641 cacattcaaa ggctcaccgg ctgctatctc cacctgtttt tttactaaat cgattcctgt
    17701 gaccatttct gttactggat gttccacttg gattcgagta ttcatttcca tgaaataaaa
    17761 atcgccttct tcatccatca gaaactcgat cgttccggca ttttcataat tgactgcttt
    17821 agctgctcga acagcagcgt ctcctagtgc ttctcttttt gtttgtgaga tcactacaga
    17881 aggagattct tccagtactt tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa
    17941 atgaatgaca tgaccaaatc gatcacctaa gatctgaact tctatatggc gagcaggata
    18001 aatgattttt tccaaataca tatcatcatt tccaaatgcc gctgctgctt cttgctgagc
    18061 tgaagaaaaa tgctgtggga gctcttcttt tgattgaact ttccgaatgc ctttcccgcc
    18121 accacctgca gctgctttca gcatcactgg ataaccaatt ctgtctgcta tttccaatgc
    18181 ttcttctact gtagataata caccagtact tcctggaatg actgggacat ttgcttcttg
    18241 catcaattga cgcgcattga tcttatttcc cattgcatcg atcgttgcac ttttaggacc
    18301 aataaatgtg atattgcact cttcacacat ggaggcaaat cggctatttt cagataaaaa
    18361 accaaatcct ggatgaattg cttcggcttt tgtcacgatt gcagcgctca gtacttcctg
    18421 aacattcaga tatgaatcaa cggcttttgc cgggccaatg cagattgctt catctgctaa
    18481 ctgtgtatga agtgcttcgg catctgcttc tgaatatatc gctacggttt ggatccctag
    18541 ttcgcggcat gcacgaatga tacgtacagc gatttcaccg cggttagcta tcaatacttt
    18601 agaaaacata tttcacctat ccaatcataa aggttaattc cgcttcggct acttttttac
    18661 cgtctacttt agcgattcct ttgcctattc cggctgaagc tttcactttc aatatctcaa
    18721 cttcaaggta taaggtatct cccggtgtaa ctttcttacg gaattttgct ttgtccaatc
    18781 cgccgaaata agccgtcttt cccttgaatt gttcaagaga aagtaatgca actgctcctg
    18841 cttgagccat tgcttcaaca atcaataccc ctggcataac aggttcttct ggaaaatgtc
    18901 cttgaaaaaa gggctcattg atcgttacat ttttcttagc aacgatccgt tctccttcaa
    18961 ccatttcttc cactcgatca atcaaaagca ttggataacg atgtggaatg atttctttta
    19021 tttcttgtat gttcattgtt ttccctcttt cctgctttgc ttatttttgc tgaatcctaa
    19081 agagtggctg tccatattcg acaactgact cgttttctac aaggatttct gtgatcactc
    19141 cgtcggcctc actagttatc tcattcatca gtttcatcgc ttcaataatg cagataactt
    19201 ctcctttggc aacttgatca cctacttttt tgaaagctgg tttgtcgggc gctggttgca
    19261 gataaacgat tcccacgatt ggcgaaacaa cctcgattcc atcaaatact gccgattctt
    19321 tttctttcat tatttctgga gcagttgttg cttgttcagg tatgacattg actggctccg
    19381 aaactggcaa cgtttcgttt gtttgaacca tattcatgcc tcgacttgtt ttatttttat
    19441 tcatatacaa ttcaaattga ccttcacgca aatcgaattc cgttagcgag gattgatcaa
    19501 attgactgac taattctctg atttcattga tatccaaaat tattcctccc agcgtttcat
    19561 gcacagaacc gcattatgtc cgccaaaccc taatgaatta ctcattgcat aagtaaaact
    19621 tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc tcaggatctt gtttttctac
    19681 attgatcgta ggtgggataa attgatgttc taatgccagc aaagttgcca aggcttcgat
    19741 cccgccggct gctcccaata aatgtccagt catcgatttc gtgctgctga cgtaagtatc
    19801 tctgaaattt tcacctaatg cgatttggat tgcttttgct tctgcctggt cattggctgg
    19861 tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc ggctgtacac cagcttcttc
    19921 catcgcaagc ttcattgctt tagatgcacc agaaccatct ggagtaggag ctgtcatatg
    19981 gtaagcgtca cagtttgcac cgtatccaac gatttctcca tagattttgg caccgcgagc
    20041 ttgtgcatgt tctaaagatt caagaactag aacgcctgct ccttcaccca tcacaaaacc
    20101 attccgattt tcgtcaaacg gaatcgaacc acgattagga tcagttgatt ctgagagtgc
    20161 tgttaaagct gcaaatccag caatccccat ctcacaaatc gttgcttctg ttcctccagc
    20221 aagaatgaca tcagaatagc catgtttgat gttacggaat gcttccccaa ttgaattcgt
    20281 tccgctagca caagctgtta cgatagctgt acaaatacct tttgcaccta cacgcaaagc
    20341 aatatttcca gctgccatgt tggcaatcgc cattggtacg aacattggag aaactcgttt
    20401 tgcacctttt tcatgtagac gaataacttg gttttcgatt gttggaagac caccgatccc
    20461 agatccgatg atgacgccaa accggtcttg attcattttt tctttatcta aaccgctcat
    20521 ctccattgct tctaaagcag cgtagattcc atagatcgaa aacatatcca ttcgtttgct
    20581 atctttttta ataaaatatt tatcgtaagg gaaatctttg acttcacccg ctaaggtaat
    20641 ccctgtttct gaagcatcaa atttagtgat cggtgcgatg ccattcgtcc cctcttttaa
    20701 gctattcaaa aagctgtctg cgtcatttcc aataggtgaa gtgactccat agccagtaat
    20761 taccactcga ttcatttgtt tgctctcctt ttcttatccg tgcatgacca atccgccatc
    20821 gacattgatg acttgtccgg tgatatatgg attttgtgct aagaaaactg ctgtttgtgc
    20881 aatatcttct acttgtccga aacgctgcat cggaatttgc ttttccgctt gttccttgac
    20941 tttgtctgct aaaacttccg tcatatcagt tgtgatgaat cctggcgcaa tggcattaca
    21001 tgtaatgcct ctagttgcag cttctcgtgc cacagatttt gttaatccta ctacgccagc
    21061 tttactagct gcataatttg cttgaccaat atttccaatc aacccagata cactagagag
    21121 attaatgatg gccccttctc gctgcttcat catttttttc aatacgtgct gcgtcatatt
    21181 gaaggttcca atcaaattga tatccagaca ttttttgaaa tcttctgcat ccattcgcat
    21241 cacaagtttg tcatttgtga ttccggcatt gtttactaac acatggatcg atccaagctt
    21301 ttcttctgct tctttgatca tttgtcccgc tttctcataa tcagagatgt caccggaaac
    21361 tcctacgcat tttacaccga atgcttcgat ttcttctatc ttttcttttg gaatctctcc
    21421 gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc
    21481 gattcctctt gtactacctg tgataaatac attttttcct tttacatcca taacgttcct
    21541 ccttatagta ttgctattgt ttcatcaaat gtctgcttat tctcaacacg cagaactggt
    21601 attgttttat cgattttttt cataaatccg cttaatactt ttccggggcc gacttcgatg
    21661 acttgttcga tgccgattgt ttttaactta tggatactct catagaaacg aacaggttgc
    21721 atgacttgct gttcaagtag tcccgcgatt gtttctttct tcattatttc cgttgttgtg
    21781 ttgcttatga taggaaaaga aggttcagag aattgaatct gacttaggtc cttcgccagt
    21841 ttttttgctg ccggttctaa aatagctgta tgaaaaggcc cgctgacatt tagagggatc
    21901 attcttttga accctttttc ttttaatagt aaaacagctt cgtctactgc tttttcttcg
    21961 ccaccaatca cgatctgctg aggtgtatta taatttgcag gagatacgat gccgtacttg
    22021 ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt
    22081 ttcccgcttc cagctggggc agcttctgtc atataagcgc ctctttttgc tactaacgca
    22141 actgcttcag tgaaggatag cgcattacta gctactaatg ccgaatattc tcctaaactc
    22201 agtcctaaag ctgcatctgg gatgattcca tgctccttca ataagcgata atacgcgatg
    22261 ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat ccaaacgttc attttctgta
    22321 aagcaaagtt cagccatatc gtagccgaga atctcacttg cttcatcaaa tgtctgcttt
    22381 acaacggctt cttcatataa atcttttccc ataccttgat attgtgcacc ttggccacta
    22441 aataagaatg ctgttttcat gtttttctcc aatatatcca gaatatatgt ttgattcttc
    22501 ctattcaaaa aggccgagac attcctattt caggaaatgt cccagccccc aacctaattt
    22561 taatgattat gcagtttgtt tttctacgta agtcactaaa tcttgaaccg ttttgattcc
    22621 gtcttctgtt tcgattttca cgtcaaattc atcttcaatt tcattgatga tttggaataa
    22681 atccaagctg tcagcttcta agtcttcttg gatgtcagta gtcaatttga tttcttcagt
    22741 ttcttttcct aattcttctg cgatgatttc ttgaattttt tcgaatacca taataatata
    22801 ttcctccgat attgtttgct ttttattttt ataatttaac agtctacaac tccatgagaa
    22861 tacttcccca tgttaaacct ccgccatagc ctgtaaatat tactttctgc tgttttccta
    22921 aggtgatctt tccactagaa actgcttcat ccaataagat agggatgctt gcagcagatg
    22981 tattcccata tttatccata ttggtcagga acttttcttg cggcatcttt acttttcggg
    23041 caattttctc aataattctg aggtttgcct gatgcaaaag cagataatca actggtttgt
    23101 ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc
    23161 tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc tgaatagaac ggatttttat
    23221 tttgctggta gccagcggtc aatgagctac ttctggtacc gtcagctgct aatttttctt
    23281 tcagcaattt tttctgtgga ttcgcttcta agagaatccc acctgctcca tcgccaaaca
    23341 acacagcagt cgtacgatca gaccaatcga ttagcttaga tagtgtttca ccaccaatca
    23401 ccagtccctt agttgagccg cagcggatca ttttttcagc catgcttaac gcataaacaa
    23461 atcctgagca cgcagcactg atatcaaaag cgatagcatg agttgctccc aatttccctt
    23521 gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga cattgttgct actaaaataa
    23581 aatccaaatc tttagggttt agatttttct gttttatcaa tttttccgcc actaaaatgc
    23641 ataaatcaga agtctcctga tttgttacac atcttcgttc acgaataccc gtccttgacg
    23701 aaatccattc atcagaggta tccatcatct tggaaagatc attattagta ataatctttt
    23761 caggaaggac gctggctgtt gcagtaatca ttccaaattg ttccattaaa tatcctcact
    23821 tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct
    23881 ccgtagccat tccgtctaag atgttcttga ccatttcacg atgaaaatgc tgatggaccc
    23941 gaaatagtaa ctttcctttt ttcgttagtc ccaactttac aacgcggcgg tcatcttcac
    24001 tgcggatacg ttcgacatag ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc
    24061 caacagtaat tgatagttct ttggctactt ctgaagttgt ttttttcttg tacatcccga
    24121 ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt gaattgagat tttttcagct
    24181 cagactcttc gattgtcaaa atatcattga agacactaac aagataatca tttactgttt
    24241 ttaaatctgg ttccatgttc caccgtccac taattagttt gattatcaaa tcttttgatg
    24301 ttcaaactat atcttaactt ttttcaaaat gcaaccttaa aagatcattt ttttactata
    24361 tgagcttttt ttaagatttt actcatttaa aacaactgat aaatcagcat ttataatttc
    24421 ttttattaga aaaatcatct atctcac
//
LOCUS       DAYYHS010000039        24428 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000039 DAYYHS010000000
VERSION     DAYYHS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.039"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACHA6A_001538"
     CDS             complement(55..789)
                     /locus_tag="ACHA6A_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8969736.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACHA6A_001539"
     CDS             1116..1526
                     /locus_tag="ACHA6A_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8969737.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACHA6A_001540"
     CDS             1519..2220
                     /locus_tag="ACHA6A_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8969738.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACHA6A_001541"
     CDS             2220..3806
                     /locus_tag="ACHA6A_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969739.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACHA6A_001542"
     CDS             3825..4046
                     /locus_tag="ACHA6A_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8969740.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACHA6A_001543"
     CDS             4043..4804
                     /locus_tag="ACHA6A_001543"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8969741.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACHA6A_001544"
     CDS             5184..5693
                     /locus_tag="ACHA6A_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8969742.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACHA6A_001545"
     CDS             complement(5768..6307)
                     /locus_tag="ACHA6A_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8969743.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHA6A_001546"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHA6A_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8969744.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACHA6A_001547"
     CDS             7320..8171
                     /locus_tag="ACHA6A_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8969745.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACHA6A_001548"
                     /pseudo
     CDS             8199..8834
                     /locus_tag="ACHA6A_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            8854..9627
                     /locus_tag="ACHA6A_001549"
     CDS             8854..9627
                     /locus_tag="ACHA6A_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8969746.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACHA6A_001550"
     CDS             10035..10832
                     /locus_tag="ACHA6A_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8969747.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACHA6A_001551"
     CDS             10810..11223
                     /locus_tag="ACHA6A_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8969748.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACHA6A_001552"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACHA6A_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8969749.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACHA6A_001553"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACHA6A_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8969750.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACHA6A_001554"
     CDS             16000..16560
                     /locus_tag="ACHA6A_001554"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8969751.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHA6A_001555"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHA6A_001555"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8969752.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACHA6A_001556"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACHA6A_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8969753.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACHA6A_001557"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACHA6A_001557"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8969754.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA6A_001558"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA6A_001558"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8969755.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA6A_001559"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA6A_001559"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8969756.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHA6A_001560"
     CDS             21907..22485
                     /locus_tag="ACHA6A_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8969757.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHA6A_001561"
     CDS             22537..23019
                     /locus_tag="ACHA6A_001561"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8969758.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHA6A_001562"
     CDS             23136..23510
                     /locus_tag="ACHA6A_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969759.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23940..>24428
                     /locus_tag="ACHA6A_001563"
     CDS             23940..>24428
                     /locus_tag="ACHA6A_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8969760.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         8008 a         4389 c         5213 g         6818 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaataaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 cagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatggggt attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta
    23941 tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat ttaaatgaat
    24001 tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt
    24061 ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt
    24121 cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc
    24181 gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg
    24241 aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga
    24301 tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc
    24361 tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt
    24421 ttatggac
//
LOCUS       DAYYHS010000040        24400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000040 DAYYHS010000000
VERSION     DAYYHS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.040"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1082
                     /locus_tag="ACHA6A_001564"
     CDS             207..1082
                     /locus_tag="ACHA6A_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8969761.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1227..1379)
                     /locus_tag="ACHA6A_001565"
                     /pseudo
     CDS             complement(1227..1379)
                     /locus_tag="ACHA6A_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1647..2978)
                     /locus_tag="ACHA6A_001566"
     CDS             complement(1647..2978)
                     /locus_tag="ACHA6A_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8969762.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3127..3840)
                     /locus_tag="ACHA6A_001567"
     CDS             complement(3127..3840)
                     /locus_tag="ACHA6A_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8969763.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4015..5439
                     /locus_tag="ACHA6A_001568"
     CDS             4015..5439
                     /locus_tag="ACHA6A_001568"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8969764.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5738..6445
                     /locus_tag="ACHA6A_001569"
     CDS             5738..6445
                     /locus_tag="ACHA6A_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8969765.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6432..6755
                     /locus_tag="ACHA6A_001570"
     CDS             6432..6755
                     /locus_tag="ACHA6A_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8969766.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6844..7881)
                     /locus_tag="ACHA6A_001571"
     CDS             complement(6844..7881)
                     /locus_tag="ACHA6A_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8969767.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8202..9185)
                     /locus_tag="ACHA6A_001572"
     CDS             complement(8202..9185)
                     /locus_tag="ACHA6A_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8969768.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9600..10370)
                     /locus_tag="ACHA6A_001573"
     CDS             complement(9600..10370)
                     /locus_tag="ACHA6A_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969769.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10363..11298)
                     /locus_tag="ACHA6A_001574"
     CDS             complement(10363..11298)
                     /locus_tag="ACHA6A_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969770.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11614..13263)
                     /locus_tag="ACHA6A_001575"
     CDS             complement(11614..13263)
                     /locus_tag="ACHA6A_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8969771.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13380..15983)
                     /locus_tag="ACHA6A_001576"
     CDS             complement(13380..15983)
                     /locus_tag="ACHA6A_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8969772.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15995..17044)
                     /locus_tag="ACHA6A_001577"
     CDS             complement(15995..17044)
                     /locus_tag="ACHA6A_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8969773.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17070..17384)
                     /locus_tag="ACHA6A_001578"
     CDS             complement(17070..17384)
                     /locus_tag="ACHA6A_001578"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8969774.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17400..17846)
                     /locus_tag="ACHA6A_001579"
     CDS             complement(17400..17846)
                     /locus_tag="ACHA6A_001579"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8969775.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17847..19769)
                     /locus_tag="ACHA6A_001580"
     CDS             complement(17847..19769)
                     /locus_tag="ACHA6A_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8969776.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19981..20877)
                     /locus_tag="ACHA6A_001581"
     CDS             complement(19981..20877)
                     /locus_tag="ACHA6A_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8969777.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20870..21550)
                     /locus_tag="ACHA6A_001582"
     CDS             complement(20870..21550)
                     /locus_tag="ACHA6A_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8969778.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21551..22333)
                     /locus_tag="ACHA6A_001583"
     CDS             complement(21551..22333)
                     /locus_tag="ACHA6A_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8969779.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22326..23243)
                     /locus_tag="ACHA6A_001584"
     CDS             complement(22326..23243)
                     /locus_tag="ACHA6A_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8969780.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23407..23730)
                     /locus_tag="ACHA6A_001585"
     CDS             complement(23407..23730)
                     /locus_tag="ACHA6A_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969781.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         7412 a         4932 c         4011 g         8045 t
ORIGIN      
        1 gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa cacgctattc ttttactttt
       61 tctcacattc tctaagaaaa gaaaaggaga atgaattttt gacaattcga gcaaaagtga
      121 ttatcaaata tgtaaaatca gctacttttt tcttaaacgt ttctttatac taaatataaa
      181 taagtgtttc atgaaaggag aactagatga ataattggct tgagtatttt cctgaaaacg
      241 ttttggaacg cggttacagc tatcatctgc acggttttgt ccgtcatttg aactatacaa
      301 gcaagtactt gtccgcaaca gtttctggca cggaagatta taaggtagtg atcacttggg
      361 atgaaaagac caacatgact tgtgattgtc tatatgccat agaaggaaaa aaatgcaaac
      421 acatggctgc tgtcttattc gcttacgaag aacgtccaat aaaaaaatcc aactactcac
      481 tttccgaact ctcgtccctt gtttcctccg ctagttcctc tcttgtacgg gagcttttaa
      541 cagagattct tatagaacat ccgcaattta tcgaacgctt caaagttaag atgccatttc
      601 atgcaataaa ttattcagat aaattaacaa ctatcatcca taaatacgat catattatca
      661 agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga gatgcgaaag ttcatccaag
      721 aagcagttga atcgctcatt cagcaaaatg cctatcttcc cgcattcgaa ttgatcaatg
      781 aagtgattgc tactctcgaa acattctatt gggagcctga ggatgaacga acccttcttc
      841 tgatagagga ctgttattat ttatggaaag aattattagc agaagcacct catgcagaaa
      901 aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca tactgacgcc tcttattcaa
      961 aacgctactc cataaaaatc ctgaaagaag actttcggga aaaagaattt tctaaccaaa
     1021 agaaaaaaat agataaaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat
     1081 agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa
     1141 aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca
     1201 tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctggg
     1261 tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt
     1321 tccactgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac
     1381 tctaatcttt aaagataaat attaaaataa cgattaataa tattaacagt ctttattttt
     1441 caccaccaaa aacctatctt tgcatatact aaatagacgt atatacacag ataggtttgt
     1501 cttgtgaaaa ataatgatta tccttaccaa aaaatgtttg tttttctaca tgttcgtact
     1561 tatcaaaaaa tatgatcctt attgaaatta ctggctattt atattccaac gacttcttgt
     1621 gttaattttt gttatctcat agtaaattat gtttcatttt cacttaattt agttgtcacg
     1681 tgttcgtcaa ttcgtaaaaa cggtaaatat ataaacacac ttgcggctat aattaatatt
     1741 tgtaaaacag ctgctcgcca gtctccggct gttgctaaaa atcccgatat aactggtggc
     1801 gtagtccaag gaatttgcac tactacatga ttaattaatc ccacagcagt tgcaaaatat
     1861 gctactgtca aagaaaaaat cggtgcaagc acaaagggaa taatcagact gatattaaat
     1921 acaataggca atccaaaaat aataggttcg cttatattga atatagcggg agttaccgat
     1981 aatttagcaa agtccttata atcttttcta cgactgaaga taaatattgc aatcaataaa
     2041 gcaatcgtat ttcccgatcc acccattacc gcaaaggtat ctttaaaggc catattgata
     2101 atatgtggaa tttgctcttt gtttgcataa gctgccatat tttcttgcat attctgaatc
     2161 aaaaacggat ctaacaatga acctgatatt actgcttgat ggataccaaa tccaaaaaat
     2221 agattagcaa tagatgtaat taacaaaaat cctggtaaac cagttgtaat tttacttaat
     2281 ggctctgtaa ctatcgtggt aattagctta tttatatcca tattaaacaa ttgaatgatt
     2341 ataaatgaca ataaagcaaa cgaggtaatc gtaataatga ttgggattaa tacattaaaa
     2401 gactttaaaa cagcgggagg aatatttccg gatagctgaa tctgtagttt cttattatta
     2461 gaaagtttaa taaataattc tgtagccgct aatccaacaa atatacctac aaacattcct
     2521 gaagctcctg tataggatat aggtatcaca cccgtcactt caacagcttt atccgcattt
     2581 tcaggagtaa acatcatctg cataggtgtt aatacaacaa atgtcgaaat agcaactaat
     2641 accgctgcaa tatgatttgt ataatttctg cttgcacaca tatgataaga tactgctgct
     2701 acaactaata atgtagttat gcttaatgta ccattggcaa ttgaagtacc aatttgttgt
     2761 aacgtggtca atgtactagt atcgacaatt tcccccatga atcctgttgg ttctaagatc
     2821 actgaattaa taagtgtcac aaatcctgct aacatcataa atggcataaa tgtagcaaac
     2881 gcatctcgca aagttcttaa atggatctga ttccccaact tagtcgcaac tataccaaat
     2941 ttttcaaata caatgtcctt cctgctactt ttgcccatat tttttctcct ttatgcttat
     3001 tgtaatcgat tactttttcc acccaaataa tacatcactt atcaaaaaaa gtaaatactt
     3061 ttaattaaat atatatatct tttttagtat aaaaaaataa gctctgaaaa aataattttc
     3121 ccgagcttat ttttcaacaa aaacagcttc aaaaacaaag tcttgatata aatgtctcga
     3181 taccgaatac tcgaaaggtt taccagacga tagagcaaca actttgtcta tctcaatcat
     3241 aaattcacct ggatctattt ttaatatttt tgcgtcaaat tcattcgcag tatctccttt
     3301 gatacgtatg tgcgaagtct ttatttccag acccagttca tttgtgatat aagcatatac
     3361 agatttcttt aaatgttttg gttccaagcc tggtatgact gctaatggca tgaatgtttg
     3421 ctcaatcgag tatggttgac aatcaataaa acggcttcgt acaatattat atacatagtc
     3481 ttttacactg atacttaatt tatcagctac ttttttcgat ggaagttcaa ttgagaaagt
     3541 atggatttct gaatctacgt gttcttcacc aaccacgcta aacaaacctt cgtttggacc
     3601 aagaactcgt gagttatcaa ttggtgttct gacgacttca gtcccatacc cacttctact
     3661 tcgcaaaact ccttcggaaa caagtaagtc tacgcctttc tttactgtta acttgcttgt
     3721 atgataaata actgccaatt catctcctgt aggaagtttc tcaccaattt tatactctcc
     3781 atttacaatt tttttcttta tatctaagta aatttccata tacttcgcaa tttttttcaa
     3841 tggtgaccct ccaatcaaaa acatatcttc atctatactt taacataaat aaaccgataa
     3901 aatccacatc aaaattatac acacaataaa aaagatatat atatttaaac aaaactattt
     3961 actttaagtt gtaagcggtg ttatcattaa gtggaaacag gaggtaaaaa aattatgaca
     4021 actatgaaaa agctaacttt tccagatgat ttttggtggg gttccgcttg gtcagctgag
     4081 caagcagaag gaaaaggaga aacaggaaaa gcagagacta tctgggatcg ctggtttaaa
     4141 gaacaacctt accggtttta tcaacgaatc gattcctcta ttacaaccga tcacatccat
     4201 caatataaag aagatataga actaatgaaa aaaactgggc ataactcctt tcgcgtttct
     4261 atttcttggg cacgaatgtt tccaaatggc ggtaccggaa aagtaaatcc caaagccatt
     4321 tctttctatc gaaaattatt ttccgaaatg aataataacg gaatcaaagt atttgccaac
     4381 ttatatcatt ttgacatgcc tgctactctt caagataaag gaggatggga atcacgtgaa
     4441 gtagttgatg cttatgttca ttacgctgaa acttgtttta aagaatttgg tgacttagtt
     4501 tgtcattggt ttaccttcaa tgaaccactt gggcctattt taggtactta tcttgaagat
     4561 tttcattatc caaacctcat tgactttaaa cgcggagcgc aagcagcatt cttcactata
     4621 cttgcacacg caaaagcaat acaagtattt aaagaactag ctcttcctag taaaataggc
     4681 gttattctaa atttgagtcc tacttttcct cgtagtcaaa atcctgctga tgtgaaagca
     4741 gcagaaatcg ctgatttatt ttatacgcgg agctttttag atcctatggt taaagggaat
     4801 ttttctaaaa agcttatcct gttattacaa gaatataatc agcttcctga agattatacg
     4861 aaagaagatc ttgtatgtat ctacgaaaat accgctcaaa ttcttggact aaactattac
     4921 gagccacgtc gcgtcaaatc tcgttcttct atggtggata catctagtcc atttttacca
     4981 gagtggtttt tcgaaccaca tatcatgcct ggacgacgaa tgaacgaata ccggggatgg
     5041 gaaatttacg aaaaaggaat ttatgatttg tgcatggata tcaagaacca ttatggaaac
     5101 atcgaagcat ttatctcgga gaacggaatg ggcgttgcgg atgaagaacg tttcatggat
     5161 gatacagggc aaataattga tgattatcgg attgagtata tcaaagatca tcttgcttac
     5221 ttatggaagg caataaatga aggatgtaac attaaggggt atcatctttg gacatttatt
     5281 gattgctggt catggatcaa cgcctataaa aaccgatatg ggctggtttc actcgacctt
     5341 actactcaaa aaagaacaat taaaaaaagt ggcgaatttt acaagcaatt aagtgataca
     5401 aatggattta gatacgatgt agacttgtta gttgaataat attctaaaat tccacttgaa
     5461 gtgaacacat tagaaaacaa gaataatgag aaataccaat aacaaatact ttttcttcat
     5521 acttcattta tacttgtcag tataagagac tgtgacaaat gtagtgatcc tcagaagtta
     5581 ggtctggaaa tctaacttga gaagattttt ttaaactacg tccagtcttt tttatttgtt
     5641 taaaattgaa gttgacaagt cttcattaaa aatttagaat attcacaagt ttgacgacaa
     5701 ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa
     5761 aactgcgatc aaagacacgt tacctaccgt ttttggttat atcggtattg gacttgcatt
     5821 tggtatcgtt gggaaagctg ccgggtttca tccgctggtc gtcacgttga tgtccctgct
     5881 gatctatgct ggttctgccc aatttatcac ggtcagcatg cttgctagcc atagtccact
     5941 gctctccatc gttctctcga cctttctagt caattcccga atgattctga tgagtatgac
     6001 gattgctcct tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct
     6061 gacagatgaa agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa
     6121 ctttcgttgg atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct
     6181 tgttggcgca cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc
     6241 catcgtcgct atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc
     6301 taaacgatta cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat
     6361 ttttatccca agtaacttag tgattttagt tgtaacactg attgggtgcg gattgggggt
     6421 gtggatcaaa catgccttct tttgagttta cattgctgac tattttaggt tgtactttag
     6481 caacttggct ttctaggatc ctaccatttg tcttactgaa aaaattcgat ttgccgcaac
     6541 cgttgcttga gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca
     6601 gtctatttac ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt
     6661 cagttcctac acttttagct gctattcttt caaaaagctt attggtgatc gtcttggtag
     6721 gaattctttc cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa
     6781 attactgtca tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct
     6841 tgcttatccg ttgaacggac cactaacaac gacttttcct aatatatgtc ctgtttctac
     6901 gtctttggtt gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa
     6961 tgtactgcgg ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg
     7021 cgtttcgatt tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat
     7081 agattggttt ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac
     7141 atgtccaaat ggtcggacaa ggtgtttgat ttgatcaaag tagcgggtaa tatcgaataa
     7201 tacagcgaca tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg
     7261 atgataatcg attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact
     7321 tgcagtcgcg tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac
     7381 gcccccagct ccgttgatca ctaagatttt tttcccgcgg ttggcatttt cttctggagt
     7441 cagaccaaat ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc
     7501 ggcttcttca tctgaaagat tttttggtgc aagagcgaca atacgttcgt caaccagttg
     7561 gtattcttga ttgcttcctg ctcttgtcgt tgtacctgcg taaaaaacgc gatcgccgac
     7621 agaaaatttt tgcacttcat cacctacagc aacaactttc ccgactccat caaaccctaa
     7681 gatacgtaaa gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac
     7741 agatactgcg ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt
     7801 ttcatcaata aagctttttg gatcatcgat cgacaaccct tcataaaaac cgactacctt
     7861 cattaattcc ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat
     7921 acagttttca tttcaatata tatagtatgc tatttgttcc acttttattc atcataatta
     7981 ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga
     8041 cttttgtcac aacctctttt aaacggtgtt caatcaactg accctcggta ttcgctcaaa
     8101 aaacgacaaa atatgaggag ctatttttgt ttggctgttc ttattacttg atgcaggaca
     8161 gctttttcac gacctctaat tcacggtgtt caagcaagaa atcattgttc actgaaaagc
     8221 gttggatgtt ctttttcaaa ggtgtctaag atctctcgta cttcatcagt agatttcgta
     8281 ttcatcaact gaactcgcaa gtcatttgct cctggaaatc ctttgatata gattttaaag
     8341 aaacgatgta gtcctgtgat ggaacgcggg atgatttctg aataatgatc ttgcagatct
     8401 agttgcagac gaagtagtcc gatcagttct ttttctgtat gttccctcgg ttctttttca
     8461 aatgcataag gattcttgaa aatcccccgg ccaatcatga tgccatctac gccatatttt
     8521 tcggccaatt cttctcctgt ctggcgatct gggatatctc cgttgatcgt gatcagtgtt
     8581 tgcggagcga tttggtcacg taaagcaatg attttaggga tgacttccca atgtgcatct
     8641 actttactca tctctttacg cgtacgcaaa tggacagaaa gattggctat atcttgatga
     8701 agtaggtgac tgatccattc ttccatctca gacatttccg tatagccgat acgcgtcttg
     8761 acgctgacag gcaaaccgcc agctttagcc gcttcaatca attgtgccgc tacttctggg
     8821 cgaaggatca agccactgcc ttttccacgt tctgctacat tagggactgg gcagcccatg
     8881 ttgatatcga ttccttggaa gcccatttct gccatagcaa tgctcatttc tctaaaaaat
     8941 tctggcttgt ctccccaaat atgggcaacc atcggctgtt catcttctgt aaaggtcaat
     9001 cttcctcgta cactgtcttt tccatctgga tgacagaaac tgtctgagtt tgtaaactca
     9061 gtaaagaaga catccggtgc tgctgcatgc tttacgacat ggcgaaatac aacatctgtt
     9121 acatcttcca ttggtgctaa aacaaaaaaa ggctttggca gctctttcca aaaattagta
     9181 gtcatttatt tgttactctc cattctatca agcagtgaag tcgcaatctt tctcaaaaac
     9241 attgtcatta tactaggaaa caaaaaaaga agcaaagtga acagcttaaa agatgtttct
     9301 gattgagcaa aaaaagactg tgacaaaaat cttatcacaa ttccttttcc ggaataaatg
     9361 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa acgaaaaata ggaggagcta
     9421 tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac ctctccacta
     9481 aacggtgttc aatcagctga ccctcggtat tagctcaaca aacgacaaaa tatgaggaac
     9541 tattttcccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttc
     9601 taacttctca atcctcttcc tttattgatc aaataccaag taacggcgta caaaacaacg
     9661 atgaatagca caagtacgat catcgatgtt gctactggca catcgattgt tccaaggaat
     9721 ccgtaacgga aaccggagat catataaacg atcggattga ctttcgaaac agcttgccaa
     9781 aatggcggca acatagagat ggcatagaaa acgccaccta gataagttaa cggctgcaag
     9841 acaaatgtag gcacgatcga tacatcgtca taagactgag caaaaatccc gttgagcagt
     9901 cctgctaacg aaaacaagat tgccgtcatc aataatgtaa tgatgaccat gaaccatgaa
     9961 tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc gacgagaata
    10021 ctgcgaccca ctccgccaat cacaaagccc caaatgatca catgcgtagg aacaggcgct
    10081 accaagattt cttcgatgtt tttctggaat ttttgagaga agaaggacga agaaacgttg
    10141 gcatacgaac tagtgatagc agacatcata atcaaaccag gcacgatgaa ttccatatat
    10201 gaaaaaccac ccatgtcacc gatccgacca ccgatcattt ttccgaaaat aacaaagtac
    10261 aaggatgtcg tgatcacagg cggaaccagt gtttggaccc agatacgcaa atagcggttc
    10321 gtttccttga tagctaaact ttttaatgca gtaaaataaa gattaaacat ttttctcctc
    10381 cgtatgatta ttttcttctg tgattttcaa aaacagttct tctaaacgat tcgacttatt
    10441 cctcatagaa agaactttga ttccttgtct tgtcagctga tcgaagattc cgttcacccc
    10501 ttgatttcgt tcaacttcga cagcgattgt ctgttcatct tcaaatgtac aagcgtatcc
    10561 ttcaatcaca ggtttggtct catatggcgc taaatcaaag ataaatgttt caaattgcag
    10621 tttggataga agctctttca tactagtatt ctcgatcagt tcacctgatt ggatgatccc
    10681 gatgttgcga caaagcattt cagcttcttc taaataatgc gtcgtcaaaa tgatcgttgt
    10741 accattttcg ttcaattctc ggagaaactc ccacatctcg cgtctcaatt caatatcgac
    10801 tcctgctgtt ggttcatcca agatcagcaa acgtggttca tgcatcaatg ccctagcaat
    10861 catcagtctt cgcttcattc ccccggaaag catacgtgca cggacatttc tcttttccca
    10921 aagattggac tgcttcaaat atttttcgct gcgtttcatc gcttctttgc gagagacacc
    10981 gtagtagcct gcttgattca cgacgatttg ctggaccgtt tcaaagggat tgaaattgaa
    11041 ctcttgcggg actaatccaa tctgttgctt tgctcggact agatccgtat ccagatcata
    11101 accgaagaca ttcacttttc cagatgtttt attgactaaa gaagtaatga ttccgatcgt
    11161 tgtagacttc ccagctccat ttggtcctaa tagcgcatag aaatctcctt ctttcacgga
    11221 aagatcgatt cctcgcaatg cttcgacccc acttccataa acctttttca aattatttat
    11281 ttcaagcgca tttgccattt atttcctatc tccttataga catgattctt tattgtatca
    11341 tggaatacag cagatgaaaa aacaacttgg ttccagcgaa aaatcaggtt gctgggctca
    11401 ggaaaatctc ctaatgaaag acatcaatcg gacgaagtca gacaaccttt tcagaaaaaa
    11461 caaatcgaag ataaaggatt actattcata tgtgcaacaa aaggagcgtg agacaaaagt
    11521 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcaa aagccaagat
    11581 ttttgatcca ataaatactt tcgatcactt cgcctatccc aatacatgcc actgccgctt
    11641 ttcatcaaat aacgcggctt ccgtttttgc ggtttctact aaagtctctg ggtaatccaa
    11701 aactttcagc aaggcaatcg catttcgggt ccttgaaggt ccttctttta atttgaaatc
    11761 aaaactgatt ccttttcctt ctgttacttg ttcttcaaag tggacattct cacaataatt
    11821 ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag atggatactc
    11881 cgcaagccac cgaacgactg aagcggaaga agcaatccgt tcgatcgtat ttgtcccttt
    11941 taaaatctca tctacgaagc agtagcagcg ttctttggaa gccacttggt ccaacaaacg
    12001 tttgatcgat ttgatttctg aaacaaaata actgtcgcct tcaaataaat catcttcgat
    12061 tgccatggaa gtcaacacat gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg
    12121 gatcgtctgt gctagaatac agctgatggc tatacttttg acatacgtag attttcctga
    12181 tgcattggaa cccgtgacta atgtattctt ctgccagttg accggattca ctaccgcttc
    12241 ttttaacaat ggatgataga tatctttcgc tgtcacaccg ccctcagtaa actctggtcg
    12301 gcaagtgatg ggcatataag tacggtaatt caatattgca gcggctactt ctaattctcc
    12361 cagcaattcc cacagcttga tggcttcagt tgaatgcttt tctaatttag tgagaacgaa
    12421 atgataggag ataaatggca gcatgaacat gatgttgaca tattcaaaaa tcaactctgc
    12481 ttcactccca ctcttcatac gaaatgaaaa acctaattta gggatgtctc ttaatggttt
    12541 agtttgctgt ttcagctctt cttgaagtgg tgtcggcagt tttgctagct gattggcggc
    12601 tgaaatcgtt gaaaccaaat aactcatgct attcagctct gtttctagtg tttgtttctt
    12661 cactagataa tagaccgtat tgaatactaa cccaccgatt gccaaataga ttcctagtac
    12721 ttgtcctaaa agaagcaaac ccaaacccac cagcggaagc aagccaagaa aaataaataa
    12781 acctacgcgt ccaatctttt tactttttcc atccgctaga tattgttttg caaaattatg
    12841 atcttttttt cctaaacgag cgaattgata ttggattttt tctctcagtt gaggattttc
    12901 ctgataaaaa gcaatcagtt tttctaactg ctgatcttca ccaaaatcaa agctgcgtaa
    12961 acgttgatac aacgcttctg aacctacact agaataggta ctgttgattc cttcgaatac
    13021 ttcgaacata tccaggtcat accaagtaag atcatcgatt tcgctatccc atgaacggaa
    13081 cttcttctcc gtgagccatg cttccttcaa gctttcttct ttatcaaatc tcggttgata
    13141 aggaattttt tcccatttgc tacgaactgt ttcctttaat ttcaaacgtg aaaaaatgtc
    13201 taaagcaact aataaaataa cagctgcaat aataattaaa acgataaatt ctctttgacc
    13261 catatccctc tccgttcctt tatttgttag ctttagtata gcactgttgc gctatttgaa
    13321 aagagggtaa aaaaacacct ttcaaaataa aaggtgtttt tttcttgtat attacatatt
    13381 tatctctcat ttatcaaaat tgacaaataa tcatagggag gaattgttgt taattcttgt
    13441 ttattctcca gtgcattgac aactgtccca agggaaagga tagcctcagg tactattttt
    13501 tcttgttctg ttggattagc taagcgaaga acaaatcctt gttcatgaaa ataagaagga
    13561 taaatcgatg atacaattaa gtcttcaggt aattcaatca atgaaaattt acgaggaaca
    13621 tcaagttcat tttgtaatgg ccaaatcttg ttatctaacc tgtgaataaa tacattcagt
    13681 ttctgctttt gataagaaat agattgttct aaccgttttt ctgcctgttt tgccactttg
    13741 aactcattaa atcttccttc ttctacatta aatccaaaag tgactttata ctcacctatc
    13801 tcttgagcta gcggtgtttc catcatgaca tgcccttcat ttgtcgtatc tcctgaagct
    13861 cgcccaggtc gccaagcaag attaggtttt ccaagttccc ctgttgttga aaataaggtt
    13921 atgattaatt gattttctat acgttcatac tcttttaaac catctgcata aaacgtaaaa
    13981 taatgatcat tttctgccac agagacactc ttatcgaata tttctaaatt aacaggtttc
    14041 tctaccatag tttctgacca attcggagga atgacatcag gggtattttc aataaatccg
    14101 ttttgaattt gtgcaattga tttttcagat ttatctaaaa cattaattgc taagcgcata
    14161 cgatgcgagt aaacagtatt atgaattttg agtgtgcctt caacgatttg actttctccc
    14221 agactgaacg atatttcata agatatttct tccatttcgg gtttttcaga taaacgatct
    14281 tttaaatctt taggaagttg agcttttccg taaacaacta atgtttcttg taaagaatct
    14341 ttatatactt tagctgtttc taattttaga gatagttctg tatctccttc taatggtgaa
    14401 taatcatacg tatcgccatc atttgctgaa tcgatcaaat gaacgaaatc aagatattgt
    14461 ctatctccaa ctatcaaatt caattgccca tctttaaaag aaagtttata tttattgtta
    14521 cttatctcag ttttgtctat ctcgattgct gtttctaatt tttcattatt tttctcaaat
    14581 tcaacaacag tgtatcctag tgctggcaca tttacattta tttttatgtc aagttcataa
    14641 taagctggtt catcaaaata ttcgaaacct gtagcaatca tccgataagc atttttccgt
    14701 tctggaaaat aaacttcttt ttcaatgata ttatagggac tacctttgaa gtgtattttc
    14761 tttgaatcag ctactacatg cacgattttc gatccactat aattctcagt cgttgtgttg
    14821 aaaacaagga cttcattcga ttttagcttt aatgcatcag ccatccgttt tacaataagg
    14881 ttttcgattc cgtcagctat ctcattagct tctttgattc gatggaaaat atcttccgca
    14941 acattatctg aaacacagcc accaatacta tcatgagcct ggttttgcaa aatctttttc
    15001 cataaccgac gaagtaggcc tttactgata tggataccat ttctttctgc aatcagcatg
    15061 agcggctcaa ctcgttttag aattttttgt tccaatttaa aattttctat tttcattttt
    15121 gttcttactg agccaataga acgatgaaca cgagagtaaa ccggctgacg aagttcacct
    15181 ttatattcag gtaattcttt tttgtttctg ataacttcta cataagaagg ataatctgaa
    15241 ataatattgt ggaatttact gtatttattc aacttctcta cgacttgatc aaaatttaaa
    15301 atcattgact tttgatctac acccgaaggt actaaaatat ctttgttttt tccttgcata
    15361 tgaagcattt caactgcttc atccaacttt tcttttgcaa attcaggaat atctttaatt
    15421 gcgtctaagg tcatcattcc agtcatataa ccaaatggaa aattcatggc ataaacaaat
    15481 ttatctccca atcctttcca gataaaataa ggagatggaa ccatcgtttc aaagtttaat
    15541 cctcgccaac acatgaaatt atcgatttct acttgatgca aaagtgtagg caaattcgca
    15601 ttgaacccaa acgtgtcagg caaataccct accatcattg gatctccata tttaaggatc
    15661 gtatcactga ttccaatcat taagtttctt aaaattgatt cagcatccac taataaggca
    15721 tctgtttgtg cataccacgg tcccacgaat agttgtcctg ttttcatcag tcgttggata
    15781 accttaagct tttgcggatt gatctccaaa tactcatcga cgattgaagt ttgtccatct
    15841 aaacaaaagt ttactttagg gttcctttcc aattcatcta gaacttcagt aaagacctga
    15901 tcagacaata cgatggcatc ttcatttgta aaataccatt ctctgtccca gtgcgtgtgt
    15961 tgaattatat gtgcatttac catatcagat tctcctactt tccttcttct actgaattta
    16021 cttgttgttc ttttttatac ttgatggatt ttagtgcatt gaccattaac gctgtaacca
    16081 ctcccccaat aacaattgat actaaatata aaggtatatt cgtaacaaaa ggaattgcca
    16141 taaaggttga aagcattgtt tcttgagtaa caccaaaaaa tccatttaga ccacatgcta
    16201 ctgcagtgcc caccacgatg cttggtatta ctcgtaaagg atctttcacg gcaaacggga
    16261 tcgtcccttc agtaatcccc acaaggccca taatcatagc tgtctttgca ttatctcgtt
    16321 ccgattcatc ataaagtttt ttgtttaaaa ttgttgctaa aaacattcca aatggcggaa
    16381 tagcaattcc taataacatg gctgtactag gtccaaagct tccgcttgaa aaagaagcaa
    16441 ccccaaaagc atatgcgatt ttattgatag gtcctcccat atcaaacgcc atcattcctc
    16501 ctaaaatagc cgacatcaaa gcagcatttg ctcctgacat gccttctagc caattagtca
    16561 gtcctgttgt cattgcttga ataggcgtag ctagtacata ccataatatg attccaacaa
    16621 ctaatgtagt aacaaaagga atcagcataa ttgattttag actaataact tgcatcggta
    16681 cttttacttt tttcaatgca ttagctaaat agccacaagc taatccagca accattcctc
    16741 caataaatcc cataccaaga ttttgacaca tcatcccagt gataaaagct ggtgctagac
    16801 cgggacggtc agcaattgaa tatgcgatat aggctgaaat catcggtacg ataaactgta
    16861 atcccgtctg accaatcatt cgcaggactc cccagaatgt atccgtctgg tcccaaacac
    16921 ctgtgccacc aaaaatgact gcaatagaag ttagtaaccc tgctgctacg acaagcggga
    16981 tcatatagga aaccccactt tggaaatgat ttagaatttc tcttccttgt ttcttgaatt
    17041 ccattagtaa ctcccccacg atatattttt tatttgttaa ttaatgcaat tgcttcatca
    17101 atcgtctctt caccatcttt tactacctct gaggtcccta ctgttaaaat cggtttaccg
    17161 tcaaagcgac ctcgttctga aacatttgta tctactgcaa atatgacaac atctgcctcg
    17221 gaaacatccg aaattgaaag tttgttttct atccccatag ccccctgtgt ttctacttta
    17281 atatctaacc ctttcttctt cgcaaacttt tctaaattag cttttgccat ataagtatgg
    17341 gcaatccctg cgatacatgc tgttactgct actatttttg tcatatgaaa ttcctccttt
    17401 taaaatgata gagcttttac caattctttt ttatttctcg aatcttttat tcttttaaca
    17461 aactcgtcat ccatcaatct tccagataat tctgctaaaa tacgtaaatg attagtacct
    17521 ttatcagtgt cttttattgc taacaaaaag acgagatcaa ctggttgatc atctaatgcg
    17581 ccccattcaa cagaccgctt taacttggct acagccactg ttgattcaat tactgctagg
    17641 cttttgccat gaggaatggc gatatttttc ccaattcctg tggtcatatg gttttctctg
    17701 tcaaatacgt ctttttgaaa ttgtttgtgg tcagacacaa acccattagt ctctaacaat
    17761 ttcgataatt tttcgactac acttttttta tcagatgcat ttaaatctat cactatatta
    17821 tttatattga ttttttcact gatcatttat aagccttctt tcaatatata aactaatttt
    17881 tcaaacgacg aactcgtttt taaatttgct aataactcat gattcgaagc gatttcattg
    17941 aatgtttcat aaattttatt caattcaggt tttacttttt cattaagtgc aaaaaagaaa
    18001 acagaatgaa caagtgtccc atcccaatca attccttctg gattaacgta gacacagatt
    18061 actggctttt ttataaaatc tacttcgata tgcggcatag caaaatcatt catcgctgtt
    18121 gaagatagtt tttctctttt gattgctccc tctagtatcc cttctttcgc gtaattttct
    18181 tttaccaatt ttttggtaat tatatttaaa acgttcactt gattatcctt tatttggtgg
    18241 gtaaaaaagc aaaatcgttt atctaataaa tttagaaaag catcttctgt ttgttcattt
    18301 aactgtagat actgttttac ttttctgaca tctgccccta acatcaaagg actaacaaca
    18361 ataacaggaa aatttgtatt ttcaatcgga atcgtgctta taacaagttc atcagtgact
    18421 tttctatctt gtagttcatt gatgcttagt acatcttcta tctcaaccat atctgcaaaa
    18481 ccgttattta ttctttgctc taataatttt gatgttccat atccgctact acaaactaat
    18541 atcactttcg ttttgtcagg tttgtaacga tccaaaagtg attgaatatg gagtgttacg
    18601 taagcgattt catcttcatt aaaatgtata cagaatctct cttcgatttc ctctaataaa
    18661 tctacactta tcataaatga ccttttaaaa gaatatttga ttttgtccgt ataagggtta
    18721 tagatattta ctcccaattt gagtctttta actgctgcat tgagatgtaa tactagatta
    18781 ttgatcaatt cttcatcagg ataaaattca tttaatttaa ttcttactaa ctcttgtagc
    18841 tcaacaccca tatctaaata ggaaagcttc gtttgatcta agtcgtgatt catagcattt
    18901 ttttcaatcg aagtaatgtg aatgtcaaca taagcttgtt catctattgg cacaataatt
    18961 gaaaatgctt tttcaatcat tttgattaaa ataagtgtat tctttgagat ttcttcttca
    19021 gaattattac tacgtgataa gaaattgctt gtttcaatcc tttctatcgc aatagcaagg
    19081 tggatagcta aagtttgaaa ttcataatct gtaaatttca attgtgtctt attcatgtat
    19141 cgattaagca attcggctac ttttttcaat atgtccttat ttaagaaatt attcgcttca
    19201 ccaatgaaat tgatattcaa gtctaagaga tctgttttac tgttagatgc tgtaaagcca
    19261 ccccaataat agttcacaac ttctgacatc aaccttcttt tacttttttc atcagctttg
    19321 atgcgcacgc catttcgatc tgaaactaag gacatcccat gcttgtttag tctttttcta
    19381 acatctttca ttgtgctttc taccgtcgac ctgctcacgt ataattcgtc agcaagattt
    19441 tgaatcttta gagaatcatt agtattcaat aatttagcaa acacataagt tagacggtcc
    19501 tccggagagt ctgcgatatt gattttatga ctggataata aatttcgaag tgtttctttt
    19561 tttccaataa gcgaaacgcc ttttttaggt tgaaccaaca tttgtacatc tggcattttt
    19621 tggattattt ctttcaaaat taaaatatct ttgctaattg ttttcgtaga tacatataat
    19681 tgttctgata attctttgta agttgtgtgc tcattttcaa tcaaaaagta tagtattttc
    19741 atttgccgtt cgctaaattc gatgcccatt atcttcacct cctgaataca gcttaagtgt
    19801 acaattatta tctctatttt catacagaag aaagttcttg attaatgtag aagtttctaa
    19861 tattcttgat tatttcaaat caataaaaaa gcctgcaata tttatatcac aggctttttt
    19921 ctctattaaa tatatataag ataatacatt tttctactag ttctcactaa tcttgtataa
    19981 ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc tagcggtcat
    20041 ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc cgctagattc
    20101 cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt caacagaggt
    20161 ttgcgttcct ggtttgatag gattggccac tgttaatcga atgaatggat cctcttttga
    20221 aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga tattttgcag
    20281 cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt gttcagcgaa
    20341 cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata cttcttccac
    20401 ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct ctattcgagc
    20461 aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt tgatgatcgt
    20521 taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta cataaccgtt
    20581 aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata gtctgatgtt
    20641 ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaacc atgtattcag
    20701 tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac gaccaccata
    20761 ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt ccctttgcag
    20821 gaagaaaata aaacttccca taaagataac aacgagaagc aacagccatt taatcaaatc
    20881 taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct agcttccctc
    20941 gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt tcttgccaaa
    21001 ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa aaaagcttca
    21061 gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt acatccctcg
    21121 actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct tttacttgga
    21181 cagaagtctg atgacgaagt tggatgccga ttcgtaacaa aagttcttcg atatcaaatg
    21241 gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct ctttgatcgc
    21301 tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct gctaacaatg
    21361 cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca ggatatgtgc
    21421 ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat ctctgttcaa
    21481 gaacctcctc cagtaaggaa tgaacagctt catcgtcttc gataatcaaa atagtttcca
    21541 gcaggaccac ctacttaatg tcagattttt ggaaaataaa cattcctaga gctgatgata
    21601 ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa gttgcttgtt
    21661 gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga aattccaagc
    21721 ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc catgatataa agaaaagaaa
    21781 gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga acagataatg
    21841 tccctaatgt cagcagaagc aatacaggaa atacttttag aacataattt ccatagtttt
    21901 tgattccctc aacttcacca aatccgaaag caagtgtgta agcaagcatg gctagtcccg
    21961 tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc gacacataat
    22021 aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt tgaaattctt
    22081 ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca gccatttttt
    22141 ctaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg tttaattcat
    22201 tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga agtacgacca
    22261 tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt tcagcagtta
    22321 atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc cagtttttgc
    22381 ccaactaggt gaataccatc cacaggaact tttgcttcta ccaatgcttg attgatggct
    22441 gctacttggt caattccttc aaaaagctgg ataacacggc tagattgtac aaaataatcc
    22501 ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt gtcaattttt
    22561 ataaattgac gagattcttg tgccaattct tctttcgata attctttgat caagtgtcct
    22621 tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt catctagcaa gtggctagaa
    22681 agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg catttctgcc
    22741 atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc tggattcgtg
    22801 atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt ctttgctttc
    22861 tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc tctatccgac
    22921 acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc tggataaaga
    22981 gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc cgtcgttcca
    23041 aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct cataaaggta
    23101 gacttacccg ctccattttc cccaatcaat ccatagatca ttccacgttt gatttcgatt
    23161 gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt tgctttcaag
    23221 accgtttcat ttccaaaatc catacttatc cttctttctt attgatgagt gaagtatagt
    23281 tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt tccaattgaa
    23341 tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt atcaggcagt ttgttatacg
    23401 cttgtatcag tttcctgatt ctttcttttc gaccaaagtc gtttctctgt attcctcttc
    23461 actgaaaatt tttgcttctt tcagatcaag cttgagtaat tttaccagac cggttataag
    23521 gataactata gcaagactcc ctcccagaaa aattagactg tctcgcagac gtggttgcgt
    23581 tgtcttccaa tcaaagagcg caccaaacag tgctaatcca agtggactgg ccaccatggt
    23641 aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc gttggaactt ctcgctgaag
    23701 ccagaccata taaaaagcaa aagaaaacat taatccttaa aaacggactt atgaaaaata
    23761 atacatgccg tttgggattt acaattagct accaaataag aggttatgac aaaagacttt
    23821 tcacaatccc ttcttccgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
    23881 aatatttatc aaacatgaga agcaattttt gtaaattcct tcttatttct tgaagctgac
    23941 cacttctgtc acaacctcag aagcggataa ataatattgt ttgatgatac tttatctttt
    24001 tcccgtttgt ttatagatcg aatgatatta tagaccaaga atcctaccta ttaacatagc
    24061 taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg ccagaaaagt taggattaag
    24121 gtaactgcgc tcagcattgc ttcgcccagt acgattctcg taactcgtga actcgaacga
    24181 taatcgcaat taacgttttg agaactgtga agctttacgg gctttcttaa gacctggttt
    24241 tttcttcgta tcattgaata agtttgaaaa accttgattt ataaaggttt tcccgtaaat
    24301 atattttatt atatttgatt aaatttgaaa tgtttgtgtt ctaaaatgtg ttctaattta
    24361 aaggcagtat atttcgtatt ttagattaaa tttttaggct
//
LOCUS       DAYYHS010000041        24259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000041 DAYYHS010000000
VERSION     DAYYHS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.041"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHA6A_001586"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHA6A_001586"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8969782.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHA6A_001587"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHA6A_001587"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8969783.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHA6A_001588"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHA6A_001588"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8969784.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHA6A_001589"
     CDS             complement(3373..4680)
                     /locus_tag="ACHA6A_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8969785.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHA6A_001590"
     CDS             complement(4683..5264)
                     /locus_tag="ACHA6A_001590"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8969786.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHA6A_001591"
     CDS             complement(5836..7128)
                     /locus_tag="ACHA6A_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8969787.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHA6A_001592"
     CDS             complement(7125..7748)
                     /locus_tag="ACHA6A_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969788.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHA6A_001593"
     CDS             complement(7751..8587)
                     /locus_tag="ACHA6A_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8969789.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHA6A_001594"
     CDS             complement(8604..9491)
                     /locus_tag="ACHA6A_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8969790.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHA6A_001595"
     CDS             complement(9507..10790)
                     /locus_tag="ACHA6A_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8969791.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHA6A_001596"
     CDS             complement(11473..12156)
                     /locus_tag="ACHA6A_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8969792.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHA6A_001597"
     CDS             complement(12174..12791)
                     /locus_tag="ACHA6A_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8969793.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHA6A_001598"
     CDS             complement(12801..13625)
                     /locus_tag="ACHA6A_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8969794.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHA6A_001599"
     CDS             complement(13618..14178)
                     /locus_tag="ACHA6A_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969795.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHA6A_001600"
     CDS             14352..15308
                     /locus_tag="ACHA6A_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8969796.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHA6A_001601"
     CDS             complement(15613..16569)
                     /locus_tag="ACHA6A_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8969797.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHA6A_001602"
     CDS             16673..17275
                     /locus_tag="ACHA6A_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8969798.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHA6A_001603"
     CDS             complement(17363..18862)
                     /locus_tag="ACHA6A_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8969799.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHA6A_001604"
     CDS             complement(18878..19402)
                     /locus_tag="ACHA6A_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8969800.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHA6A_001605"
     CDS             19700..20449
                     /locus_tag="ACHA6A_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8969801.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHA6A_001606"
     CDS             20440..21225
                     /locus_tag="ACHA6A_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8969802.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHA6A_001607"
     CDS             21194..21910
                     /locus_tag="ACHA6A_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8969803.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHA6A_001608"
     CDS             complement(22012..23031)
                     /locus_tag="ACHA6A_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8969804.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHA6A_001609"
     CDS             complement(23050..24051)
                     /locus_tag="ACHA6A_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8969805.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5221 c         4225 g         7625 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgatttt
//
LOCUS       DAYYHS010000042        23687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000042 DAYYHS010000000
VERSION     DAYYHS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.042"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..540
                     /locus_tag="ACHA6A_001610"
     CDS             19..540
                     /locus_tag="ACHA6A_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8969806.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            637..858
                     /locus_tag="ACHA6A_001611"
     CDS             637..858
                     /locus_tag="ACHA6A_001611"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8969807.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1015..1179)
                     /locus_tag="ACHA6A_001612"
     CDS             complement(1015..1179)
                     /locus_tag="ACHA6A_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM8969808.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="ACHA6A_001613"
     CDS             1282..1803
                     /locus_tag="ACHA6A_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8969809.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2230
                     /locus_tag="ACHA6A_001614"
                     /pseudo
     CDS             1790..2230
                     /locus_tag="ACHA6A_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2270..2428)
                     /locus_tag="ACHA6A_001615"
     CDS             complement(2270..2428)
                     /locus_tag="ACHA6A_001615"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM8969810.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="ACHA6A_001616"
     CDS             2521..3282
                     /locus_tag="ACHA6A_001616"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8969811.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3469..4821
                     /locus_tag="ACHA6A_001617"
                     /pseudo
     CDS             3469..4821
                     /locus_tag="ACHA6A_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4837..6057
                     /locus_tag="ACHA6A_001618"
     CDS             4837..6057
                     /locus_tag="ACHA6A_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8969812.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="ACHA6A_001619"
     CDS             6092..6769
                     /locus_tag="ACHA6A_001619"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8969813.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="ACHA6A_001620"
     CDS             6766..7962
                     /locus_tag="ACHA6A_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8969814.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="ACHA6A_001621"
     CDS             7977..8300
                     /locus_tag="ACHA6A_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8969815.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="ACHA6A_001622"
     CDS             8304..8639
                     /locus_tag="ACHA6A_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8969816.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="ACHA6A_001623"
     CDS             8636..9031
                     /locus_tag="ACHA6A_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8969817.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="ACHA6A_001624"
     CDS             9028..9399
                     /locus_tag="ACHA6A_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969818.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="ACHA6A_001625"
     CDS             9415..9975
                     /locus_tag="ACHA6A_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8969819.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="ACHA6A_001626"
     CDS             10102..10413
                     /locus_tag="ACHA6A_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969820.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="ACHA6A_001627"
     CDS             10437..10628
                     /locus_tag="ACHA6A_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969821.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="ACHA6A_001628"
     CDS             10628..14473
                     /locus_tag="ACHA6A_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM8969822.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="ACHA6A_001629"
     CDS             14474..15346
                     /locus_tag="ACHA6A_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8969823.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="ACHA6A_001630"
     CDS             15343..16392
                     /locus_tag="ACHA6A_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969824.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="ACHA6A_001631"
     CDS             16396..17220
                     /locus_tag="ACHA6A_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969825.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..19370
                     /locus_tag="ACHA6A_001632"
     CDS             17238..19370
                     /locus_tag="ACHA6A_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8969826.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19387..19536
                     /locus_tag="ACHA6A_001633"
     CDS             19387..19536
                     /locus_tag="ACHA6A_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969827.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19533..19979
                     /locus_tag="ACHA6A_001634"
     CDS             19533..19979
                     /locus_tag="ACHA6A_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969828.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19981..20118
                     /locus_tag="ACHA6A_001635"
     CDS             19981..20118
                     /locus_tag="ACHA6A_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8969829.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20155..20448
                     /locus_tag="ACHA6A_001636"
     CDS             20155..20448
                     /locus_tag="ACHA6A_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969830.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20471..20668
                     /locus_tag="ACHA6A_001637"
     CDS             20471..20668
                     /locus_tag="ACHA6A_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8969831.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20679..21698
                     /locus_tag="ACHA6A_001638"
     CDS             20679..21698
                     /locus_tag="ACHA6A_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8969832.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21955..22602
                     /locus_tag="ACHA6A_001639"
     CDS             21955..22602
                     /locus_tag="ACHA6A_001639"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8969833.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22927..23073)
                     /locus_tag="ACHA6A_001640"
                     /pseudo
     CDS             complement(22927..23073)
                     /locus_tag="ACHA6A_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            23312..>23687
                     /locus_tag="ACHA6A_001641"
     CDS             23312..>23687
                     /locus_tag="ACHA6A_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8969834.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAK"
BASE COUNT         8284 a         3973 c         5195 g         6235 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtcaaa
    18721 ttgactggct caagatcgaa aaaggcaata ctcgaacccc gaatattagt gagtataaat
    18781 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    18841 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    18901 caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta
    18961 atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta
    19021 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accacattaa
    19081 cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta
    19141 tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa
    19201 ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt
    19261 ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa
    19321 actcaagtta tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga
    19381 atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg
    19441 aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat tctaaattgg
    19501 aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta
    19561 ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag
    19621 atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa
    19681 tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt
    19741 taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga
    19801 tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag
    19861 aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata
    19921 gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt
    19981 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
    20041 gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat cattagcaaa
    20101 gaagaaagcg catcttaagt gatgcgtttt tatttaaggt aaaggagttg tcacatgatc
    20161 aatttagggg aatggggaac aatcgcagga tcaattactg cgattgtttc tttgatttta
    20221 ttagtaataa gaccagtcac tgcatctttc tcgaagatta ctgaaactct ttcaaaagta
    20281 agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga tcgattgatg
    20341 attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta
    20401 gaacacacac aacaaattaa aactttattt agagaaaggt ctcggtaaaa aagaaaggag
    20461 ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga cagttttaac
    20521 agtattgcca gctttatctg tattagtagc cacattaggc aaagcatatg gatggaatgg
    20581 aacagatatg gcagttctga ctatcaatgc agcagcaaca tttttaggtg ttatcactgg
    20641 tgtgtcggct tataatttga aaaaatagga ggaaacaaat gaaaaagaaa attactatta
    20701 ctgcgatgag cctattaacg gctctttttt tattgccgat taatgggttt gcctatacga
    20761 ttaacaatga atttaattta ggtgtaaacg aaggtagttc tcaagtagca aataatcagt
    20821 acattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
    20881 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
    20941 atcaagtcgg tcaacctggt tatgtacagt acggtgctgg ttcgtatgct aatgcaaata
    21001 gtcctgtgca gattgagtta caacacacac atgataaagc aacgtttgag aaaaactaca
    21061 aggcatacgt tgaattagct agagattcag caatgaaata tggtattcca cttacactag
    21121 acacaccgta taatcaacca gggatcaaat cgcatttatg ggtaacacaa aatatttggg
    21181 gcgatcatac agatccttac ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag
    21241 cctatgattt ggctcatgga tttaccgatg aaaatccgac aacttcagat gataaaccag
    21301 tcattgatcc aactcgagca ggtgctgcaa atcctaccct gacagatgga acaaattacg
    21361 cccacattga tcagttcgga gaaatcgaaa acgcaaactt acatgtggct ggttggcaca
    21421 tcgctaacta taaatacgag tatatcttca ttatggatta caatactggg aaagaattag
    21481 ctcgagtaaa agctgatgga atatatagac cagatgtaaa tcaagcttat aatactttag
    21541 gaaatgttgg ctatcatgta tcttttaaca tgcgcaactt ccctaataag aaagtctatg
    21601 taatgatgcg ggcaacgaat gatccagaag ggaacactaa aggcggagca caagattttc
    21661 atgataaacg ctggtattta aatattccgc aacgataaaa atagcccctc gtttttgagg
    21721 ggaagtacat aatgcaaatt tgtccataac ttattgatta tcttgtaaaa atgcggtaat
    21781 ttaatagtta gtgagaagag gtgcttaatt atagttaaaa aagaggaaat agaagcagca
    21841 tgtaaccaag caaatgttag gtttattgga gaagctttat ctggatatcc taagtataaa
    21901 tacttacaca actggttaca aagtaaaagt ttttatctta ataaagaaag atgcatgcct
    21961 aataaaaatt atagggttta taaaagagga actattgtat atgttgattt tggggtgaac
    22021 gtgggttatg agttatctgg taatcatttt gctattgttt taaataataa agataattcg
    22081 aaaaatggtg ttgtatgtgt agtaccaatc tcttcaaaaa agaaaagtaa ttatgtatct
    22141 gtaggaaaaa tattagaagt tgctagtatt aaacaattta taagccaagc tgataaatta
    22201 aaagagaaac ttaggataat tattttactt tcattaaata gaggcatcat tgatgaaagc
    22261 gatttgaata gttttattaa agatattgaa gctaaaggtg aaaagctttc agtggaagaa
    22321 gtagaaaaac gagctgtaaa acataatttg gtttgcgaga caagggaaca agtaattaaa
    22381 gaaataaaat cattatctga agatatggta aaatatcaga aggtgtttaa ttgctacaag
    22441 aaatatacac aagaatccta tgtaatgcca cttaatatcc aaacgattag taaaaataga
    22501 attagaagaa ttaacaagtt tgatccttct ggaaatatga aggcacctgg aaatgtcctc
    22561 aatgaagtag atgaaagttt aaaaaaatat tttacaaagt aaacgatatt tttgttgaca
    22621 tttttctgtt cgttagttac catgtagtta tagaaggacg taagcgccta tttttaatgt
    22681 acacatcggg acgtatgcgc ccacatagaa acatagttga gagacatcta tatggtgtct
    22741 ctttttttat taatatgcaa aaaagtctag ttttttcaat aatatttatg taataataaa
    22801 ttagccacca caacaaagaa tgaaatccat tattatctag tctagtccat tctttttgtt
    22861 tgcagtattt gtgatggctt tccgtaccct tagctcagtt ggtcagagca gacggctcat
    22921 aaccgtccgg tcgtacttct cgtaatccag gaaggccgtc agctccgtcc gcagtagcgt
    22981 gtttacggcc agctccagat gggagcggaa taactcgttc aaatcacctt tggtggctag
    23041 agtctgcagg agttctgtaa taaaatcgtt catagggaag tcctcttttc tgtgaatggg
    23101 tgtggtaact ttattctaca gaagggactt ccttttttct atttacacaa aatattttat
    23161 actctcaaat gtttgtatgc agttacatct gactttattc gaattaagga aaagatttat
    23221 aaaaataaaa taaaaaaata tatatttaaa ttgacttatt gtgaaatcat taactataat
    23281 tagttcagtt tatgttaagg agaatgatga tatgaataag aaaattgtag taagtggatt
    23341 aattataggt ggattagtgt tagcagggat cgcagggaaa aactttgccg atgacatttt
    23401 gttatggggt ggagaagata atattgaaca aatcaatcat aatttggagt cattagataa
    23461 agcactagaa aataaagagc aaaatattag tagtttaaat tcacgattat cttcaaataa
    23521 tcaagaactt gagcaagcaa aggctaacgt tgaagaatat aaacaaaaag tgttgacttt
    23581 ggaaaatgat aaaaatcaat taactataga gaaaacaaat ttagagaatc aattatcagg
    23641 aaaaaatgct gaattgcaag gtaaacaaaa tgaaatcaat gctaaaa
//
LOCUS       DAYYHS010000043        22827 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000043 DAYYHS010000000
VERSION     DAYYHS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.043"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACHA6A_001642"
     CDS             1..369
                     /locus_tag="ACHA6A_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8969835.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACHA6A_001643"
     CDS             549..1490
                     /locus_tag="ACHA6A_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8969836.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACHA6A_001644"
     CDS             1559..2290
                     /locus_tag="ACHA6A_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8969837.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACHA6A_001645"
     CDS             2434..4614
                     /locus_tag="ACHA6A_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8969838.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACHA6A_001646"
     CDS             4634..5116
                     /locus_tag="ACHA6A_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8969839.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACHA6A_001647"
     CDS             5132..5893
                     /locus_tag="ACHA6A_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8969840.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACHA6A_001648"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACHA6A_001648"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8969841.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYDVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACHA6A_001649"
     CDS             7665..8150
                     /locus_tag="ACHA6A_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8969842.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACHA6A_001650"
     CDS             8143..8826
                     /locus_tag="ACHA6A_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8969843.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACHA6A_001651"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACHA6A_001651"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8969844.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACHA6A_001652"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACHA6A_001652"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8969845.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACHA6A_001653"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACHA6A_001653"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8969846.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACHA6A_001654"
     CDS             13123..13455
                     /locus_tag="ACHA6A_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8969847.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHA6A_001655"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHA6A_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8969848.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACHA6A_001656"
     CDS             14281..15654
                     /locus_tag="ACHA6A_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8969849.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHA6A_001657"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHA6A_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8969850.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACHA6A_001658"
     CDS             16709..17149
                     /locus_tag="ACHA6A_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8969851.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="ACHA6A_001659"
     CDS             17293..17994
                     /locus_tag="ACHA6A_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8969852.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="ACHA6A_001660"
     CDS             17991..19112
                     /locus_tag="ACHA6A_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8969853.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="ACHA6A_001661"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="ACHA6A_001661"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8969854.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="ACHA6A_001662"
     CDS             20491..21399
                     /locus_tag="ACHA6A_001662"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8969855.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="ACHA6A_001663"
     CDS             21434..21706
                     /locus_tag="ACHA6A_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8969856.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="ACHA6A_001664"
     CDS             21717..22763
                     /locus_tag="ACHA6A_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8969857.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7698 a         3980 c         5009 g         6140 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatga tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 ttaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aatttta
//
LOCUS       DAYYHS010000044        22520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000044 DAYYHS010000000
VERSION     DAYYHS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.044"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..226)
                     /locus_tag="ACHA6A_001665"
     CDS             complement(29..226)
                     /locus_tag="ACHA6A_001665"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8969858.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(372..1250)
                     /locus_tag="ACHA6A_001666"
     CDS             complement(372..1250)
                     /locus_tag="ACHA6A_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8969859.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1289..2407)
                     /locus_tag="ACHA6A_001667"
     CDS             complement(1289..2407)
                     /locus_tag="ACHA6A_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8969860.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2404..4503)
                     /locus_tag="ACHA6A_001668"
     CDS             complement(2404..4503)
                     /locus_tag="ACHA6A_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8969861.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4554..5375)
                     /locus_tag="ACHA6A_001669"
     CDS             complement(4554..5375)
                     /locus_tag="ACHA6A_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8969862.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5372..6181)
                     /locus_tag="ACHA6A_001670"
     CDS             complement(5372..6181)
                     /locus_tag="ACHA6A_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8969863.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6202..6696)
                     /locus_tag="ACHA6A_001671"
     CDS             complement(6202..6696)
                     /locus_tag="ACHA6A_001671"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8969864.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6708..7208)
                     /locus_tag="ACHA6A_001672"
     CDS             complement(6708..7208)
                     /locus_tag="ACHA6A_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8969865.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7273..9969)
                     /locus_tag="ACHA6A_001673"
                     /pseudo
     CDS             complement(7273..9969)
                     /locus_tag="ACHA6A_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(10397..11470)
                     /locus_tag="ACHA6A_001674"
     CDS             complement(10397..11470)
                     /locus_tag="ACHA6A_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8969866.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11467..12294)
                     /locus_tag="ACHA6A_001675"
     CDS             complement(11467..12294)
                     /locus_tag="ACHA6A_001675"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969867.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12295..13101)
                     /locus_tag="ACHA6A_001676"
     CDS             complement(12295..13101)
                     /locus_tag="ACHA6A_001676"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8969868.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13113..14198)
                     /locus_tag="ACHA6A_001677"
     CDS             complement(13113..14198)
                     /locus_tag="ACHA6A_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969869.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14215..14757)
                     /locus_tag="ACHA6A_001678"
     CDS             complement(14215..14757)
                     /locus_tag="ACHA6A_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8969870.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15015..16004
                     /locus_tag="ACHA6A_001679"
     CDS             15015..16004
                     /locus_tag="ACHA6A_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8969871.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16001..16738
                     /locus_tag="ACHA6A_001680"
     CDS             16001..16738
                     /locus_tag="ACHA6A_001680"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8969872.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16735..17286
                     /gene="coaC"
                     /locus_tag="ACHA6A_001681"
     CDS             16735..17286
                     /gene="coaC"
                     /locus_tag="ACHA6A_001681"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8969873.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17302..17934
                     /locus_tag="ACHA6A_001682"
     CDS             17302..17934
                     /locus_tag="ACHA6A_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8969874.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18019..18666
                     /locus_tag="ACHA6A_001683"
     CDS             18019..18666
                     /locus_tag="ACHA6A_001683"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8969875.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18671..19654
                     /locus_tag="ACHA6A_001684"
     CDS             18671..19654
                     /locus_tag="ACHA6A_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8969876.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20057..21571)
                     /locus_tag="ACHA6A_001685"
     CDS             complement(20057..21571)
                     /locus_tag="ACHA6A_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8969877.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21709..22515
                     /locus_tag="ACHA6A_001686"
     CDS             21709..22515
                     /locus_tag="ACHA6A_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969878.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         6800 a         4833 c         3904 g         6983 t
ORIGIN      
        1 atttatttta tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga
       61 ttatgaagtc aacatttcga aaaatgaaca tacattggta agcaactata actattacca
      121 cagacataaa gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca
      181 aggtatacag cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat
      241 ttaatgaaaa caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat
      301 ccaacttcat ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa
      361 acattcatta attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg
      421 attattccgg cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt
      481 tcaacatatc catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct
      541 tgtttcatta caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg
      601 tatgcacatt gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa
      661 ttctggcctt ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa
      721 caatcttggt ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac
      781 atatgcccca tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca
      841 cctccgatac ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca
      901 gcaacatatt caccatagca agccgcattg acatctgttg tccattcaat cggaatattg
      961 aaatattgct ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat
     1021 gtgatatgac cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc
     1081 ccgatgcctg ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt
     1141 tcaactggag ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg
     1201 actgcacaga caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc
     1261 tcccttcgaa tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat
     1321 gacgtttagt atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt
     1381 cttgccgctc aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat
     1441 cttcggtaaa acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg
     1501 cgtcgtcacc aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg
     1561 catctctatt tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg
     1621 gacgtccgaa ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac
     1681 ctgttgaggt tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt
     1741 cgccccacca ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt
     1801 gaccctttaa tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat
     1861 gccaatttgc gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta
     1921 tcgtataaaa aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg
     1981 atgtttggaa aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg
     2041 gcaacgagtt cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac
     2101 tttcaacagc agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa
     2161 caaaactgcc aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg
     2221 ttccagtaat atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac
     2281 ctttgcggct tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg
     2341 gattttcggg agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta
     2401 tactcatctt tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt
     2461 tttcgtatca cttattttca atggcttttc tgacaaattt gaaatactca actcggattg
     2521 cgtacattca atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa
     2581 attatcaaca ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact
     2641 gatcattagg ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg
     2701 cacgacttct ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa
     2761 atctgccaaa caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc
     2821 gctcattaaa acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat
     2881 gcctttgatt tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt
     2941 ctgatataaa tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga
     3001 ttcttctcca tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa
     3061 gattaaccct tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc
     3121 ctcatcaccg attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt
     3181 acaaattgat tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg
     3241 ttctagggct tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct
     3301 gccggtatga tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat
     3361 tggttcccct gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag
     3421 aacctgcgga caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc
     3481 cagtaacgca ctggataacg aattactcag agaatgattg atacctcctg taaattttga
     3541 accaggaaac ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg
     3601 ttcaaacatg gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt
     3661 agcaggatag ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact
     3721 agaaaagctt tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga
     3781 cagctgccca aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc
     3841 agattcctga aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa
     3901 gcttgccact tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt
     3961 ttgctgatcc gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct
     4021 ttcgtgtgtt tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc
     4081 cagtatggga gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct
     4141 aggcaaacag tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta
     4201 gacaatcccg tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt
     4261 gacccaaaca cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg
     4321 aatcgtcccg caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa
     4381 tccctttaca tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc
     4441 gtcgtttaac tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt
     4501 catcttttcc ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg
     4561 attccaagta atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg
     4621 ccgacttttt tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct
     4681 ttaaaaatac tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag
     4741 ctcaaaacgg tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc
     4801 aagatactag cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata
     4861 tatttagagc ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt
     4921 atattgaaca taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg
     4981 atcgctagtc ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca
     5041 gccaatggtc ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct
     5101 ttttcagaat tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga
     5161 atggtcgtcg aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt
     5221 ttgtagaaat gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc
     5281 accggattca ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt
     5341 ttctcatctt tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt
     5401 caagcgttgc atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt
     5461 gtagatctct ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca
     5521 atggtaactc gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt
     5581 tactccaaag catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga
     5641 ctgttaatcc gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg
     5701 ctccaaataa aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca
     5761 taaagaagtg cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt
     5821 tagcagccat cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata
     5881 ctgctagtgg aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga
     5941 acgctgtagc cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga
     6001 cgacacccat aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc
     6061 ccatcaacgc tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga
     6121 acatttcatg tccagcaact ccaataaaaa cggccaaagc gactacaaat gcatcataca
     6181 taatttttct ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc
     6241 catcgccggt gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc
     6301 ttgaatttct gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg
     6361 cggtgtcatt ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt
     6421 cgcaactttt accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc
     6481 ccgcggatct tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt
     6541 cgatggtgca gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct
     6601 tgctactact aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg
     6661 aattaatcgt tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct
     6721 tcctcttcat ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat
     6781 ttatttacca gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg
     6841 agcagaattg gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag
     6901 gttttggtaa tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga
     6961 tcctgctcgt tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc
     7021 acataagcat taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta
     7081 agaaagcctg aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt
     7141 cctttctttc tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt
     7201 ttaatcacgt taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact
     7261 tttttagaca ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc
     7321 ataagcattg actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac
     7381 ataataaaat tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt
     7441 ccgagccaag aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac
     7501 acggccattt aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc
     7561 actcatcacg atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa
     7621 gaattttact agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa
     7681 aatatgctcc aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg
     7741 aattgaaaag gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata
     7801 agtttcatca ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc
     7861 gatggattca tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa
     7921 acccgacata caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac
     7981 attgattgaa tacttgtctg ctgcatcctc cgcaatctat ttaaatggcg cattcatcgt
     8041 catcttcaag ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc
     8101 cccttttaaa tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat
     8161 cagcactaga ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat
     8221 ctctgacaca ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa
     8281 ttgattcact actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc
     8341 cttcagctcg atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag
     8401 aaagttctcg tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta
     8461 agtcgtccga ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata
     8521 gcttggggaa atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa
     8581 tccgtacgtt tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt
     8641 tctactcatt gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt
     8701 ctttatcgaa gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc
     8761 caaaatcgaa tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt
     8821 tatcaataaa ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat
     8881 caagatctca tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc
     8941 acaactgatt ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa
     9001 cattaaagac aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt
     9061 ttgaaaaaat aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg
     9121 caacataaca ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt
     9181 ttccgtcgtg gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac
     9241 tggacgatag tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag
     9301 gcgatggacc tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt
     9361 atccttttca gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag
     9421 ttcgggattg tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat
     9481 aatttgctca tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc
     9541 cgtggctcca tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca
     9601 tttttgaatg acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt
     9661 tatttgctcc gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt
     9721 tcccggtaat ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt
     9781 ctgacgagag atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc
     9841 tgttagtcgt atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc
     9901 cagtttaatg tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt
     9961 gtattccaat ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatctgacaa
    10021 aataatttat ctaccaattt agtttttatc taatatgatt tttatgtttt ttcatagcta
    10081 caaatattga actggattca tatattagtt ttattaatag attacagaag ggttaagtat
    10141 atttgcatag tttccaatta ctttatcctt ctgtaacttt ttcaatctta gatttactag
    10201 cccgtctcgg gccctatttt acaatggttt tgtccacttt tctctggtag aaaaacgtgg
    10261 tagaaaaaat aaaataacac aaaaaaagcg gcgtttcatc cgcttttggt agaagattgg
    10321 aaaactcaga tagaaaaagg acgattttta gctctttaga acgttgataa gtccccattc
    10381 ttactgagtt ttgccattat ctcctataca tcttgaattc taagaatagg tcattataga
    10441 tgcctaggta tttcgatcct agatctttgt agacttcaag atgggagatc gtgtcatcag
    10501 atggatagaa ttgttcgtcc gacgaaatgt cttttggcaa taacgctttt gcttcttcat
    10561 ttggtgtaga ataaccaata tactctgcat tttgtgccgc attttccggt tctagcataa
    10621 agttgatgaa atcataggca ccttctttat ttttcacggt ttttggcata acgatattat
    10681 caaaccaaag attggatcct tctggtggga tcacataatg taaatgttca ttttcccaca
    10741 tcatatcggc agcttcgcca gaaaatgtca ctgccacgga ggcttcttca ttgatcatat
    10801 acattttgat ctcatctgcc acgattgctt tgacatttgg tgttaaatga ttcaatttat
    10861 ttgctgcttc tgtcagctgt gccatattct tactattcaa agaatagcct aaactattca
    10921 aagaaagccc cataacttct cgtgcaccgt caatcagcat caaactgtct tttaattccg
    10981 gcttccacaa atcatcccaa tgcttaattt gatttgcatc aaagaattta tcgttatata
    11041 cgatccctaa agttccccaa aaatatggaa tcgaatactg attatctgga tcaaaatcca
    11101 agtctaaaaa tcgtggatca atattattta atcctttgat cttcgaatga tctaatggaa
    11161 gtactagttt ttctttgatc attttttgga tcatatattc acttggtata gcgatgtcgt
    11221 agtttgtccc accttgctgg atttttgtga acatcgcttc attagaatca aatgtttcat
    11281 aattcacttt gtatccatat tctttttcaa atttcgtgat caaactagga tcgatataat
    11341 ctccccagtt atagatggta agtacctttg ctccactcat gccacttgtt ttttcaagtt
    11401 gacgaacacc aagcagcagc accgcaataa tgatgagaat acctaaaaac agagattgta
    11461 attttttcat cgcagccgtg ccaccccctc gcgtactctt ctgtttttct tcagccgttt
    11521 tggacggttt tctttactaa taaagtagta tccaatgacc aatatcatgg agaacaagaa
    11581 aactaacgca cttagcgcat tgatttctaa actgattcct tgtcttgcac gagaatagat
    11641 ctctactgag agcgtcgtaa agccatttcc tgtgacgaaa aatgtaaccg caaaatcatc
    11701 aagagaatag gtaaatgcca taaaatatcc agctatgatt cctggagaaa gataaggcaa
    11761 aatgatattt ttgactactt ggaaattatt agcaccaagg tctctagccg catcgaccat
    11821 tgaatcgttc atttcctgca attttggcaa gaccatcaag acgacgattg gaatgctgaa
    11881 agcaatatgg gaaagcaata cactctgaaa acctaatccg aatcctagta gtgtaaagaa
    11941 aatcaagaaa ctagcaccga taataacatc aggagagact aataagatgt tattcatgct
    12001 caacaacgct gtccgtgcac gtcgtttttt cgtgtaatag attcccatag ccccaaatgt
    12061 cccgatgatc gtggcgatca atgcagataa gaaagccagc atgaacgtat tcaatacgat
    12121 aatgatcaaa cgtgtatctt caaaaacagc ttcataattt tcccaagtaa atcctgtaaa
    12181 gctgttcatc gttcctcctt cattgaagga ataaaaaatc agatagaaga tcggtgcata
    12241 taggaataaa aagacgatcc ctaaataaag atacgaccat ttaaattttt tcatttatga
    12301 gcgacctcct tttttcttct cgcctgtcag gagcatcacg atgaacatag caatgattaa
    12361 aatcacaccg attgtagaac ccatccccca gttttgtgta accaagaaat gctgttcgat
    12421 agccgttcct aaagtgatca cccgatttcc gccaatcaga cgagtcagca tgaataaaga
    12481 aagagaagga ataaatactg cttggacacc acttttcaca ccatttaatg ataacggaaa
    12541 aatcactcgt cggaaggtct caaagctatt cgcgcctaaa tctctactag cactgatcaa
    12601 agaaggattc atctcttcca atgcattgaa aattggcata atcataaatg gaatctcgat
    12661 atacgttgcc acgaacagaa aactgaagtc tgtgaataat atctgtttcg gcccgatccc
    12721 gataaaatca aagaaatgat tcacgctgcc gtgcacactg aaaatcccaa taaatgcgta
    12781 agctttaagc agcagattca cccatgtagg caaaataatc agcatcagcc ataattgctt
    12841 gtgcttcagt tttgttaaaa aataagcagt cgggtaactg atgatcagcg tgaaaaacgt
    12901 gatcaaaaat gcataccata cagaattgat cgtcatagaa agcgatgtcc ccgacgtaaa
    12961 ataagataga taattattca gcgtgaattg cccattcata tcaaaaaaag attgataaat
    13021 gatcattaat acaggcgcga tcacgaataa accgagccac aaaatataag ggacggaata
    13081 gatcctgcgc attgttttca ttcttgtccc ccctattctt cgtagctgtc aagacgtgca
    13141 tcgaattctt cttcagactc gttgaaacgc atgacatgga tatcttctgg ctcaaaggcc
    13201 aatcctactt tgcttccttc tttagctttt ttcgttgaat ggaccatcca ttcattttgc
    13261 tgttcatcat aacagatgat ttcataatgg acaccacgaa acagctgagt atcgaccgtt
    13321 acgactagtt ttcctttatc tggtgtcgtg atcgtcaaat cctctgggcg taaaacaact
    13381 tcaactggtt catttagacg catcccgcca tcgacacatt caaattgctt accaacaaac
    13441 tcaactagat tatcttcgat cattacaccg tccacgatat tgctctcccc aacaaaatca
    13501 gcaacgaaat gattgattgg ctcatcataa atatctacgg gtgtaccact ttggacgatt
    13561 ttccctttat tcatgacaaa aatctcatca ctcattgcca acgcttcttc ttggtcatga
    13621 gtaacaaaaa taaacgtgat tcctaatcgt tgttgcagat cccgcagttc atactgcata
    13681 tctgtacgta gtttcaaatc aagcgccgat aacggttcat ctaatagcaa tactttcggt
    13741 tcatttacaa ttgctcgtgc aatcgctaca cgctgtcttt gaccaccaga catttcacta
    13801 atctcccgtt tttcgtatcc aggaagctgg accatacgca aagcatcttt taccttcttt
    13861 tcgatatctg atttggacat tttcttgatc ttcaatccaa aagcaacgtt atcaaaaaca
    13921 ttcatatgag gaaaaagtgc ataatcttgg aagactgtat ttacttgtcg cttattagct
    13981 ggcaaatcat tcaaccgttt cccttcaaaa tagatgtccc cttctgtagc ttcggtgaat
    14041 ccagctataa tacgtaggat cgttgtttta ccacagccgg aaggaccaag cagtgtataa
    14101 aattttcctt gttcgatctc aaaacttact ttttttaata ctgtttcatc atcatagcgt
    14161 ttcactactt gatcaaatga aatgattggt ttccccacgt ttttaccccc tatattacaa
    14221 atatgattgc gttgcaacaa tcaatacttt tgttcgtttt ttgagatgat tccttaattg
    14281 atgttgtttt gtcgcttgat aatagatcga ctgtcctgct tttgccacat agaccgtctc
    14341 tcctaagctt aactctaatt ccccttctaa tacaaaaata aacgtttcag ataatgaagg
    14401 ttcaaacgtc ttatattctc ctgcaccttc aaaagtcagc aagacaggct ccatttcttt
    14461 ttcattcgaa tcagccacga gccattccaa ttcatacccg ttttcttcat cgaaataaac
    14521 cgtatgatct tcttttgaat aaaccacttg cagattcgct gcttcttgat gaaaaaattc
    14581 ttctggagtc acacctaaga cttcaagaat agaaaagaat gtttccattg atggtgaact
    14641 taaatctcgt tccaactggg aaatataccc ttttgacaaa tctgtgcgtt cgcctagttc
    14701 ttcttgtgtc aaattctttt ggatgcgcaa atttcttaac ttctcaccaa tttccataat
    14761 ttcctccgaa tcgaagtttt ctaatagtaa acttcctgtt tactaccact cgtattagta
    14821 tacgaaattt attgaaaaag gcaacccttt gtaaaatttt ttttgctgaa tatttcttta
    14881 tcttttggaa taaattctag ttgtttgatt ctttattttt cacctaattt ttgtctaaat
    14941 ttacggaaga acaagaataa aagagtaggc tttatggtat gataaaagaa aattttaaaa
    15001 ggagtgtctc acgaatgaac gcttttcgat cttttcaggt aataaaggaa cctgttttcc
    15061 atacagcaat cagtgatctg ccatttactg agttggctga aaatgaagtc ttggtaaaaa
    15121 ttgcctattc tgatgtcaat tataaagatg cattggcaat gagcgagtct ggtcaggtga
    15181 tacgaacata tccgatgact ccagggattg atttatccgg aactgtcgtc caaagtaaca
    15241 atccacgttt ttcaaaagaa gatcttgtac ttgctactgg ttttgggctt ggcgtcaccc
    15301 atccaggcgg ttatagccaa tatcaaaaag taccaggaga ctggttagtt cctctaccaa
    15361 aaaatatgac gctgcgtcaa gcgatgatcc ttggtacagc agggtttact gctatgttgt
    15421 gtgtgaacgc tttaatccgt caaggtatga cctccgataa aaaagttgtc gttactggtg
    15481 cttctggcgg tgtaggcagt acagcgatag ccctgttaca taaactagga ttccagtcaa
    15541 tcattgcctt ttccagaaaa gaagagtctg tcacatggct aaaaagtcta ggtgctttgc
    15601 aagtcgtacg acctgatgaa tttctgccag agacaacaaa agctttagga aaacagcaaa
    15661 tcgactatgt catcgacaca gtaggtggcg agcagctaag cagtcttctt ccattgatct
    15721 cttataacgg tgcagtagct ttatgtggca atgctggggg aatcaaacta aacgcgactg
    15781 tcttgccttt tattctgcga aacatccagt tgattggaat tgattctgtc aatgtaccaa
    15841 tagatcaacg tctttcttta tggcaacaga tggcggattt gcagattgca gatgaactgg
    15901 tcgtccaaga gattactctg gatcaattgc ctgaaacagc atctaaacta cttgctggaa
    15961 cacaccaagg aagaacacta gtaaatgtag gtgaccataa atgaagattc taatcacagc
    16021 tggaggaacg actgaaaaaa tcgatcaagt acgtgctatt acaaatcatt cgacaggacg
    16081 attagggcaa gcattagctg attatttagc tgccaatccg gataccacaa tcgattacgt
    16141 gactacgaaa caagcactga aaccgaaaag acactccaat ataacgattt atacgatcga
    16201 atctgcactg gacttatttt tacaattaga agaactaacg aaaaaagaac actacgatgc
    16261 gattatccac agtatggcag tcagtgattt tactcctgct tttagttttt cagaagaaca
    16321 gctggcaaaa aacctaccca cttcttcaac gcaagaagaa ctggacaatt ggtttgcgga
    16381 aaatgaacag acagatacca ctgtttcaaa gatctcttct aacaccgagc atcttgtgct
    16441 cgttttgaag aaaacaccaa aaatcatttc ttatctacgt gaatggcagc caaaagcaaa
    16501 aatcattggt ttcaaactat tagtagatgt accaaaggaa tctcttctgg cagtcgcaaa
    16561 aaatagtctg ataaataaca aaactgattt tattttcgca aatgatttga cggaaatcca
    16621 tggcgagaca caccacggct atctcttatc taaagatgga acagtggaag aagctcaaag
    16681 taaatctgag attgctgcat tgattactga gaaaattaga ctggaggaat caaaatgaaa
    16741 aagatcttac taggcgttac tggaagtatt tctgcttaca aaagcgctga tcttacaagt
    16801 caattcgtca aattaggtgc ccaggtagat gtgattatgt ctaaaagcag cacacatttt
    16861 atcacgccat taaccttgca atctctgtcg aagcgcgcag tccatacaga cgtcatgcaa
    16921 gaatcaaatc cgcaggtaat caatcatatc gaactagcaa aacaagctga cttgtttctc
    16981 atcgcgcctg ctacagcaaa cattatcggc aagctagcaa acggcattgc agacgatctt
    17041 ctttcaacag tcgcaatggc gcttccagtg ggaacaccaa aattgattgc ccctgccatg
    17101 aacacgaata tgtatcaaaa cccgatcact cagcgtaatc tggaaacatt gagatctgtc
    17161 ggctatcagg aaattgaacc aagagaatct cttttagctt gtggagattt tggaaaaggt
    17221 gccttagctg atattcagac gatcatcgaa caggtcatgc tgactcttaa tgaaagaaat
    17281 gcttagaaaa aaggagtact tatgaaaaat acaaaaaact ttacattaac tgcgatgttt
    17341 ttagctattt tgattttatt agctgttacg cctttaggtt ttatccctat cggtccaatc
    17401 aatgcaacaa ccatgcatat tcctgtaatc attgcttcga tcgttttagg accaagacta
    17461 ggtgcttttc ttggcggtac atttggactg atcagcatga tccgtagcac agttatccaa
    17521 acacctttgt cttttgtctt ttctccgttt atcccagtca tcggaacaga acatggaagc
    17581 tggaaagcct tgctgatcgc gtttattcct cgcatcttga ttggggtggt tccttatttc
    17641 gtttataaag gtctgcgcaa actaatgaaa caaaaagcag attccgtttc tttattctta
    17701 gctggacttt ccggttcatt gaccaatacg attttagtga tgaatatgat ctacttttta
    17761 ttccagcaag attatgccaa agtcatcgga acaaatttaa atgctgttta ttcagctatt
    17821 ctagcggtta tttttacaag tggtatacct gaaggaatcg tcgcaggtct tgcgactgca
    17881 gcagtatgca atgtcttgtt aagatacttc aagcaccctt ctcttactaa ctgatagtca
    17941 tcgtagccga tctctgattt ttttctcttt tggtgttact attgaatgga aagatttgac
    18001 gtcgaaagga tgatccaaat gaaaattttt gttgtaggtg caaacggaca aattggccga
    18061 cacctgataa aagatttagc tccaagttcc catgaaattt ttgcaggggt gagagatgtt
    18121 gcttcccaaa cattagtaaa aaaagagaat gtttcgtatg tctcttttga tttaacttgg
    18181 tccgtggaaa aaatgaccga agcattcaaa ggcatcgatg tattgatttt tgctgccggc
    18241 tctcaaggaa agaatcttct gcaagtagac ttggatggcg caatcaaaac agtgatcgca
    18301 gctgaaaatg cgcatgtctc tcgctatctg atggtcagtg ctgtttatgc agatgaacca
    18361 gcaaaatggc ctgagtccat gacagattat tatattacta aacattatgc agatgaatgg
    18421 ctgaaacgaa ctaacttaga ttttgtcatt cttcaaccag tcacattgac taacgatgaa
    18481 gaagttactt ccattcaatt aactaagcct aatgagaagg catcaaaaac gatcactcgc
    18541 tctacagttg ctgctgtact tgccgctcta gtggaagaaa cggatatttc tcgtacgact
    18601 ctagtacttt ctgaaggtag caaagagttg aatacagcat tccaagaatg ggcaaaggag
    18661 gaatagatcc atgcgtgcca tttcaatcaa gcatcctggt tctgtagatg tattagaaca
    18721 aattgaagtg cctgttccta aaccaaaaca aggcgaatta ctgatcaaag tccatacagc
    18781 agctgtcaat cgaacagata ttatgaaaag agaatcagtc tctttgaaac ctccctaccc
    18841 tattttggga gtcgaagtag ctggagaggt agttgagaat aaaagtgata tctccacttt
    18901 tactcctggg acaaaagtat acggattagt caatctaggt ggttatgccg aatatgctgt
    18961 gatgcctgcc gatcgtgcaa tccttctccc tgatacgcta gactatgttt ctgctgctgg
    19021 aatctcagaa gtttttttga ctgcttatca aacactttac tggcttggaa aattacaaga
    19081 aggcgaaact gtcttgatcc atgcaggagc aagtggtgtg ggaacagcag cgatccaatt
    19141 agctagacag ctttctcacg cgaaaatcat cgttactgca ggatcagaag aaaaactcgc
    19201 cttttgtaaa gaacttggtg cagatgaatt gatcaactat aaaaaacaag actttcaaga
    19261 agaggtacga aaaatcactg aaggtcgggg agtcgatgtt attttggatt tcatcggcgc
    19321 ttcttactgg gaaaagaatt tagcttctat agccgttgat ggacgttggg tcttgattgg
    19381 tatgctaggt gggaccatcg tgcctgagat agaccttggt gtattgattg ggaaacgtgt
    19441 ccaactgctt ggtacattat tgacacctag aagtgatcag tataaagcag agctcgcaca
    19501 aacttttatg caaaaagctg ggccttttct agaaaatggc aagattcggc caatcattga
    19561 ccgtacattc caattcagcg cagtcaaaca agcgcatgaa tatatggaag caaacaaaaa
    19621 catcggaaaa atcattttga agataactga ataaaatcat gctgatcaaa aaagaggctg
    19681 ggacagaagc gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt
    19741 tttgtgaatt tcagcttatt tccgaaggaa tttcttctgc ttccgccgtt tatacgtgtt
    19801 tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttttcc gaataaacgg
    19861 tgttcaaaag cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagaagctgt
    19921 ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttcactaa
    19981 acagtgttca atcagctgac cctcggtatt agctcaaaac gctgatttca caacctcttt
    20041 ttatttacgt tctatttcat tcattttccg aagcaaatcg agctgcttgt atgcccgctt
    20101 gtcgcccaaa aataatgatc tccgcaactg aatttccacc gatccggttg tttccatgta
    20161 agccacctgt gacttcacca gcagcatata accccctaat tggctggttt tctttatcta
    20221 atacttcagt gttcgtattg attttcacac cgcccatcgt gtaatggatt cctggtgcga
    20281 ttttgatcgc ataataattc ggagacgata agtcatgttc catggctgtg gcacggttga
    20341 attgagcatc ttttttctcc gcaacatccg tattccatcc atcaactgtt tttgctagtg
    20401 tttcttttgg taaatcgatt ttttctgcta attcttccat tgtctttcca gttgtgacat
    20461 atcctttttg gtcataaaat tcaatagctg tggcacgatc acgcacgcct tggtcaaaaa
    20521 tcaaataagc tgatttttca ggcaatgctg taattgctgc agatacttta tcccttgtat
    20581 ccatttcatt gacaaatctt ttcccctctt gatcgaccaa gattgctcct tctccgcgaa
    20641 caacctcacc aatcaagacc ccctcatcct gctgcacagt tggatgaatt tgtattttat
    20701 ccatatcaac ggtttgtcca cctaattttt caatcatggt gataccatct ccagttgatc
    20761 cttcttgatt tgtcgtaata taatcagcta aatcgggacg atatttttca ataaattctt
    20821 tgctggcacc aaaaccgcct gtggtaacta cgattgcttt tccggtgacc tcttttggtt
    20881 cttctccttg aacatgtaca gtcacttcat tgaccgtttc cccatttttc ttaatgtctg
    20941 tcacatctgc gttgacaaaa atagggattt cccgctcatg gacgttattc aataacccct
    21001 ccactaagta tccaccaata gccgagccat cagacggacg atgtgtcctc ttttcactca
    21061 tccctccagt gatcgttaag ttgtccagtt tgataccgtt ttgatctagc caatcgaccg
    21121 catcagcaga gtgatcgaca aagtatcgaa gcatttcttt atcattcgtt cctccaccac
    21181 cttttagcgt ttcttcatag aaggcatcat tcgagtcttt gattccttct tctttttgaa
    21241 attttgtttc tgaagcattc ataccgcttg atgatttaga tgtgtttcct ccggcaacag
    21301 gcatcttttc caaaattact ggattcaacc cagcatcttt tgcttcgata gctgctgcca
    21361 tccctgcacc accagctcca ataatcacga catcataaga ttctttcatt tcttttggat
    21421 ctgtatatac ttgttcagaa gcgcctgatg atacttcggc agattccttc gtactagaac
    21481 ttgtctgcgc tgtatttttc tgatctgcgc cgcatgcgct catagcaaga agtgcaacgg
    21541 ctgttaataa accagcaact aattttttca tttgtatccc ccttttctcc ttatactata
    21601 ttaagtgatt tttttcacaa tatcaactct gtttttcttt aagtcatata gctttggtaa
    21661 acacacgctt cttagtataa ttaaggatat acatgaagga gttattttat gaatcatttg
    21721 aaaacctttt ttacgaacag tacaaaaact gcaggtagaa tcacaaacgg aatctatatt
    21781 ttattacgta tcatcgtcgt tattatgcta gtggatcagg tctggaacca acactgggga
    21841 aatacaatgc ttttattact gacattaatt ttatttgaaa tcccttattt tctggaaaag
    21901 atattaaaga tacagattcc aaatatgcta gaaatcattt tgttatcttt tatttttagt
    21961 tcaacagttt taggagagtt aagtgatttt tatggctatt tttcaatgtg ggatactttt
    22021 ctacatgcat tcagcggatt tttagctggg ggtgtcggct tttcccttgt ttatttgctc
    22081 aataaaaata caaaaaccat gaacttgact cctttattac tagcagttgt ttccttctgt
    22141 ttttcgatga cagttggtgt tatgtgggag tttgtggaat tttcagcaga taaattactg
    22201 caattcgata tgcaaaaaga tacgatcatc cagcaaatca atagtgtgga gttaagtaaa
    22261 gacggaaaca ctgtctatca tgttccagat atcactcgga caatcattga aagtcaaaat
    22321 gcagatggta aaacagataa aacaatcatc caaaatggtt acttagacgt gggaatcatg
    22381 gatactatga aggatttatt tgttaatttt attggagcag ttgttttcag tgtatttggt
    22441 tacctctata gtcgaaacga ttaccaaaaa tttacgttca tcaagaattt tattcctaaa
    22501 aaaatcaaac gataaaacat
//
LOCUS       DAYYHS010000045        22423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000045 DAYYHS010000000
VERSION     DAYYHS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.045"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA6A_001687"
     CDS             6..305
                     /locus_tag="ACHA6A_001687"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8969879.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA6A_001688"
     CDS             378..1739
                     /locus_tag="ACHA6A_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8969880.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA6A_001689"
     CDS             1761..2021
                     /locus_tag="ACHA6A_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969881.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA6A_001690"
     CDS             2035..2787
                     /locus_tag="ACHA6A_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8969882.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA6A_001691"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA6A_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8969883.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHA6A_001692"
     CDS             complement(3539..4513)
                     /locus_tag="ACHA6A_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8969884.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHA6A_001693"
     CDS             complement(4891..5610)
                     /locus_tag="ACHA6A_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8969885.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA6A_001694"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA6A_001694"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8969886.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHA6A_001695"
     CDS             7161..8558
                     /locus_tag="ACHA6A_001695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8969887.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHA6A_001696"
     CDS             8639..9079
                     /locus_tag="ACHA6A_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8969888.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHA6A_001697"
     CDS             9227..9553
                     /locus_tag="ACHA6A_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8969889.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHA6A_001698"
     CDS             9554..10303
                     /locus_tag="ACHA6A_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8969890.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHA6A_001699"
     CDS             complement(10383..10838)
                     /locus_tag="ACHA6A_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8969891.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHA6A_001700"
     CDS             complement(11265..11540)
                     /locus_tag="ACHA6A_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969892.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACHA6A_001701"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACHA6A_001701"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8969893.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHA6A_001702"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHA6A_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8969894.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACHA6A_001703"
     CDS             14770..17436
                     /locus_tag="ACHA6A_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8969895.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACHA6A_001704"
     CDS             17800..18552
                     /locus_tag="ACHA6A_001704"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8969896.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACHA6A_001705"
     CDS             complement(18574..19728)
                     /locus_tag="ACHA6A_001705"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8969897.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ACHA6A_001706"
     CDS             19913..22354
                     /locus_tag="ACHA6A_001706"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8969898.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7199 a         3933 c         4672 g         6619 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttt
//
LOCUS       DAYYHS010000046        22242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000046 DAYYHS010000000
VERSION     DAYYHS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.046"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            307..2142
                     /gene="tdc"
                     /locus_tag="ACHA6A_001707"
     CDS             307..2142
                     /gene="tdc"
                     /locus_tag="ACHA6A_001707"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8969899.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2447..3925
                     /locus_tag="ACHA6A_001708"
     CDS             2447..3925
                     /locus_tag="ACHA6A_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8969900.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3891..5222
                     /locus_tag="ACHA6A_001709"
     CDS             3891..5222
                     /locus_tag="ACHA6A_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8969901.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5336..7888
                     /locus_tag="ACHA6A_001710"
     CDS             5336..7888
                     /locus_tag="ACHA6A_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8969902.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7895..9334)
                     /locus_tag="ACHA6A_001711"
     CDS             complement(7895..9334)
                     /locus_tag="ACHA6A_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8969903.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9309..9491
                     /locus_tag="ACHA6A_001712"
     CDS             9309..9491
                     /locus_tag="ACHA6A_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969904.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9680..10042
                     /locus_tag="ACHA6A_001713"
     CDS             9680..10042
                     /locus_tag="ACHA6A_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8969905.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10229..11086)
                     /locus_tag="ACHA6A_001714"
     CDS             complement(10229..11086)
                     /locus_tag="ACHA6A_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8969906.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11206..12117
                     /locus_tag="ACHA6A_001715"
     CDS             11206..12117
                     /locus_tag="ACHA6A_001715"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8969907.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12124..13775
                     /gene="mdcA"
                     /locus_tag="ACHA6A_001716"
                     /pseudo
     CDS             12124..13775
                     /gene="mdcA"
                     /locus_tag="ACHA6A_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13877..14176
                     /locus_tag="ACHA6A_001717"
     CDS             13877..14176
                     /locus_tag="ACHA6A_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8969908.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14166..15842
                     /gene="mdcD"
                     /locus_tag="ACHA6A_001718"
     CDS             14166..15842
                     /gene="mdcD"
                     /locus_tag="ACHA6A_001718"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8969909.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15846..16439
                     /locus_tag="ACHA6A_001719"
     CDS             15846..16439
                     /locus_tag="ACHA6A_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8969910.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16463..17539
                     /locus_tag="ACHA6A_001720"
     CDS             16463..17539
                     /locus_tag="ACHA6A_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8969911.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17564..18433
                     /gene="mdcB"
                     /locus_tag="ACHA6A_001721"
     CDS             17564..18433
                     /gene="mdcB"
                     /locus_tag="ACHA6A_001721"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8969912.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18544..19308
                     /locus_tag="ACHA6A_001722"
     CDS             18544..19308
                     /locus_tag="ACHA6A_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8969913.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19797..21890
                     /locus_tag="ACHA6A_001723"
     CDS             19797..21890
                     /locus_tag="ACHA6A_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8969914.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21915..22217
                     /locus_tag="ACHA6A_001724"
     CDS             21915..22217
                     /locus_tag="ACHA6A_001724"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8969915.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7209 a         3792 c         4734 g         6507 t
ORIGIN      
        1 attgcgaatt tttaacaata taaaaaaata aaaatatgtt tagtgaatga aggacagtca
       61 ttttatttgt ttgttcgctt tttttaggtt atctatttta gaagactgat ttattgaaaa
      121 attgttcgct tttttgcgtt gaattatcag aaaaatccga ctttatttat ttttgtttgt
      181 aaaaatctac gtggatttga tgttataaaa aaaaaattct catttttggc gctatagtga
      241 agaggtgtta cagataaaca catgtatctg caagacattg accaacaaaa tatggaggga
      301 agaaaaatga aagatatgga tatcaaggcc gtctttatag gcgacaaagc tgaaaatgga
      361 ccagtttata aaatgttgtt gaacaagatg gtagatgaac atcttggatg gagagaaaat
      421 tatattcctt cagatatgcc tgcaattagc gaaggagata agctcactcc agattatcta
      481 gccacccgcg accatatgat agaggtcttg gatgaagtca gtcagcgatt acgcgcagga
      541 tcgatcccat ggcattcagc aggccgttac tggggacaaa tgaatgctga aacattgatg
      601 cctgctttat tagcttacaa ctatgcgatg ctgtggaacc caaataatgt tgcattggaa
      661 tcttctatgg caacttcgca aatggaagca gaagtaggac aggattttgc ttcattattc
      721 aatatgacag acggatgggg acacattgca gcagatggtt ctattgctaa tcttgaagga
      781 ttatggtacg cccgttgcat caaatcgatt cctttagctg tcaaggaagt gctgcctgaa
      841 aaagtgaaaa aaatgtctga gtgggaactt ttgaatttat ctgttgaaga aattttggaa
      901 atgaccgaaa gctttacgga cgaagaaatg gatgaggtca aagcagcttc ttcccgtagc
      961 ggaaaaaata tccaaagatt agggaaatgg ctggtccctc aaacaaaaca ctattcttgg
     1021 atgaaggcgt tagatatttg cggagtcggc ttagatcaga tggttgctat cccagttcaa
     1081 gaggattatc gaatggatat taatgctttg gaaaaaacaa ttcgtgaatt agctggacaa
     1141 aaaatcccaa ttctaggtgt ggttgctgtc gtaggaacga cagaagaagg tcaagtagac
     1201 agtgtagata aaatcgtcca attaagagaa aggttaaaag atgaagggat ctatttctat
     1261 ttacatgttg atgccgcata tggcggctat gcgcgttcac tgtttctaaa cgaagcagga
     1321 gaatttgtgc cgtatgcttc cttagctgaa ttctttgaag aacatcatgt tttccaccac
     1381 tgcgtaacga ttgacaaaga ggtatatgaa gggttcagag ctatttcaga agcggattct
     1441 gtcacgatag atcctcataa gatgggctat gtcccttatg ctgctggagg aatcgtaatc
     1501 aaacataaaa atatgagaaa tatcatttct tatttcgcgc catatgtgtt tgaaaaatca
     1561 gtaaaggctc cagatatgtt aggagcttat attttggaag gatcaaaagc tggagcaacc
     1621 gccgccgcag tctggacagc acatcgtgta ttgccattga atgtgaccgg atatggtcaa
     1681 ttgattgggg cttcaatcga ggcagctcaa agattcagag aatttctcga tcatctgact
     1741 tttactgtta aaggaaaaac aatcgaagtc tatccattga atcatcctga ttttaatatg
     1801 gtcaattggg tattcaaaga acaaggctgt acagatttga acgctatcaa tgaattaaat
     1861 gaaaaaatgt tcgatcggtc ttcttatatg gatggcgatg tttatggtga acggtttatt
     1921 acttctcata ctacatttac acaagaagat tacggtgatt ctccgattcg ttttgttgaa
     1981 agaatgggtc taacgaaaga agaatggaaa aaagaacaga aaatcactct gctacgtgcg
     2041 gctatcatga ctccttattt gaatgatgat cgaattttca atttttatac aaaaaaaatc
     2101 gccaaagcaa tggaaaagaa actaaacgaa atcatccaat agaactgatt tttgtgtgaa
     2161 agaataacaa gagacatatg gagaacagac aattatttgc ttcatcaaaa caattgtatt
     2221 tccccctgtt tttcatatat tcctcgccaa gatattcttt tttataaaac gttctataag
     2281 ttggatcgtt cgtttcatgt atcaaaccca gttcactaag atcgttcaat ggatgtttcg
     2341 gtgatactgg gttttgtact gaatcgatga aaccttgtca tttctgttct tgaacaaaaa
     2401 taaaagaaaa caaaaacata tgtcgctatt gaaagaagga ataaagatga aagatactgg
     2461 ttcatcaaag caaatcacga ccatgacctt tatcggaatg acctgcgccc ttgtagcaag
     2521 tgtacgaaat attccagatg tagcagcaac gagctggacg atgatttttt acatgctggt
     2581 tgcagtatta ttgtacgctt ttccaatctc attgatctct ggtgaatttg ctggaatgtt
     2641 tccccaaaaa ggtggtccag aactttgggt atcgaatgct ctaggaaaaa aatgggggtt
     2701 cgttgtctca tggctgttat gggtccaaat gtttcctggg atggtgatgg tcgcttcagc
     2761 actcgctccg ttgtttggta atattatcga taatgtgccg cttggtttga acagcaaatt
     2821 tacattagtt gtcattttag tcgtttattg gatcatcact tttttgaatt tgaaatttga
     2881 tatggcaaaa atcggcggaa aagtcggtgt ttggttagga ttgtacgtgc ctttgacgat
     2941 gatgctctta ttagggtttg ctgcgtggat caaagttggg atcgtaccaa ccggaacatt
     3001 aggcagtttt tcatgggaca aattaatacc agatactacg actgcatcct catttgttta
     3061 ttttgcgccg atcatgttta tttttactgg tatcgaaatg tcctctgtgt atatcactcg
     3121 attggaaaat ccagttaaaa cgtatattcg cggagtattt gccgcgttaa tttttatatt
     3181 tttcgtcaat attttgaatg ctttagttgt agcaaatgtc gtaccaaaag gacaaatgga
     3241 attgaataat atcgcgcaat ctatttcgat ttattgccaa attcttggtt tgccacatct
     3301 tattgtgaat ctgtttagtt tacttgtctt cattggtgta gcagtccaat tatctgcttg
     3361 ggcctcagga ccagcaaaga cagtgacaga aagtgctaga aaaggtgcgt atccaccaaa
     3421 attcaatttt tggaagacga atcaatttga cgtatccaaa tccgttattt tgactcagtc
     3481 tgtcattatc tcaatctttg cactttttta tttgctgatt cctggcgtca atcaagcatt
     3541 tttgatgctc gtaaattcaa ctactgttat ctactgtatc gtctatgtca tcatggggat
     3601 cgcagttttg agattgcgtt atacacatgc aaagttgaat cgaccattta gaattggaaa
     3661 aaaagagaaa aagagcaatc ttggagtttg gactgtcgtt attgttttat ttgcggcaat
     3721 cggattttct gtcggactga cgatgaaagc tggaacatgg atcaatctaa ttgcagtcac
     3781 agccatcagt gtcattttgt ttgtagtacc actctgtatt gaaaaaataa agaaaccttc
     3841 ttgggaacag gaagtcgcca tgctgcttga aaaagaaaaa ggtgaacttg atggaaaaac
     3901 cggcaatgaa acggactcta actaatcgaa atatccagtt aatcgcatta ggtggcgcaa
     3961 ttggaacagg attattttta ggttcagcaa aggcaattgc atcagcaggg ccagctgtta
     4021 ttctcgttta tttgatatgc ggtttggcta tttatggttt gatgcgggcc atgggtgatt
     4081 tatttttagc aaatagtaca tttaactcta tcagtgattt cgttgactat tatttaggca
     4141 aaaagtgggc attttttgtc aattggactt attggctttg ctggcttgga gctggacttg
     4201 cggaattaac agcaatagga ttgtatatcc atttctggtt tgtacatgta ccagctttga
     4261 taagcggagg tagcgcacta atgatcttaa tggcagtcaa ccttgttgca gtcaagtggt
     4321 ttggtgaatt ggaatctttt ttctcaatca tcaagatctt ggggattttc ctttttatct
     4381 tattgggtat aggattgagt gtctcagatt ttcataccca gtctatcgga atcaaagaaa
     4441 attttggaca gatcgatgaa ttactaccgt ttggctttgc tggaatggta gtctcatttc
     4501 ctatggtttt attcagtttt gctggaattg aaatgatcgg cttaaccgtt ggcgagacaa
     4561 gtgatccgca aaagaatttg aaaaaagcaa tcaactgtct gccttggcgt attctctttt
     4621 tctatatagg aacgattttg tcgattttat ttgttgttcc atggcattgt ttagatttaa
     4681 aagaaagtcc tttagtgact atgctgcaag tgatccattg ccaaggcgga gcagcaattg
     4741 tcaatctaat gattttggtt gcttctttgt cctcagcaaa tagtgcagtc tacagtacta
     4801 gcaggatttt atatcaaaca gcaaaagaaa aaagtttgcc ccattggttt tgtgcgcttt
     4861 ctaagaaagg cgtaccttta cacggtattt taattacagg aatgttattt tttgccggtc
     4921 tttgtctaca tttttttgta gctgatagtc gattgttttt tcagcttttg gcaggcatga
     4981 caacgagttg ttttctattt gtgtggtctt cgatcttgtg tgcacatgtt caatttatta
     5041 aagaaaagaa acggaacact acacaaagat ataaagatct tgctttgttg gtgtttttta
     5101 cttcaattgc tttcagcttg ttattcgaaa gcacgacacg ctttattcct tttttattgg
     5161 ttatctggtt tgtattctta aattttgtat ataaaagaat aagcaataga aatatcagtt
     5221 aggcccagat acattttgca aaacgaatta tttcagaccc ccctgaaata atcgatacgc
     5281 ctgaaaaaac ttccctgcgt ataataacag tatcaattaa acaggagggg ataggatgaa
     5341 ctggtacaga ttgacggtag aagatgtatt gaaaaactat caaacatcaa cagaaggctt
     5401 gtcgcaagaa gaacgacaga aacgcctgac tgatcaagga tataatgaaa taaaagcaag
     5461 gcagcagaca aaacgatgga aaaaaatagc aaagcatttt actgatctgc tgatgatcgt
     5521 gttgatcatt gcctcttttt tgaaatttgc ttctagtgaa tatattgaag gaagtattat
     5581 tttattcgta gtgatcgtca atggactcgt tagttactgg caagaaagaa aagcggaaga
     5641 atcattgaat ggtttgaagc agctgatggg acaagaagcg atagtactat caaacgggat
     5701 gcaagaaaaa atccctgcga aagaactggt aaaaggagat gtcattgtct tacgtgccgg
     5761 agatgtcgtt ccagcagata tccgattaat cgaagcacat gatttattag tggaagaatc
     5821 gattttgacg ggagaatcag aagcatctga aaaaagtcat gccgttttga cagaagaaga
     5881 atcgatcggc gaccagaaaa atatgggttt ttcaggaacg cttgtacaat ctggttctgc
     5941 agtcggtgtc gttgtagaaa caggaaacac gacagaaatc ggtaaaatca atcaagcatt
     6001 gcaatcggtc gaacaacaaa cgacgcctct gattaaaaaa atcaaccgat taaacaaaca
     6061 aatttttcaa ggcattcttt gtttgagcct attcttagtt atttttacga catttcgata
     6121 tggtatggat tggcatatcc ttctttcggc tacgattgct ttggtggtgt caatggtacc
     6181 cgaaggattg ccagctgttt tgaccatgat tctatccgtt ggagtacatg aaatggcaaa
     6241 agaaaaggcg atcatcaaag gacttccttc ggtagaaaca ttaggttcta tgacagtgat
     6301 ctgttcagat aagacaggaa cattgacaaa aaatgaaatg acagttgtgg atgtagctgc
     6361 aaaagaagaa gcctgcgtgt tgtccattat gaaaaactgt caggaattaa aaacaaaaga
     6421 agaacagaaa acagaaaatt taagtggcaa tccgacagaa gttgcgctga ttcgacacac
     6481 agaaacagcc aatctacctt tgaaaaaaat agaagcaaag attccgttca gttctgaata
     6541 caaatatatg gcaacgatgc atgcggagga aaaaggagcg gtgatttacg tcaaaggtgc
     6601 accagaagta ttatttgcta aatcaactct ttcttctgca gaacaagaag catggagtca
     6661 gacagctgca gaatttgcga gaaaaggtca gcgtgtttta ggatttgctt ataaaaaagt
     6721 agattcgaaa caggaactca cgcatgagac tttaacccaa ttaacttttg cagggatcgc
     6781 cggattgatc gatccgccaa aagaaagtgc agtcaaagct gtaaaggaat gccaacaagc
     6841 agggatctca gtgaaaatga ttacagggga tcacaaagac acagcaaaag cgattgcgga
     6901 acaaattggc ttaaaacata ctgccaaagt tttagaaggg atcgatttgg atcttatgtc
     6961 tgatgaagag ttgattcagc aagtgccaat agtcgatgtc tttgctcgaa cgacaccaga
     7021 acataaactg cgaattgtta aagctttgca gaaaaatgga gaaatcgtcg gaatgaccgg
     7081 agacggcgta aatgatgcac ctgctttgaa aagatccgat gtaggaattg ctatgggaat
     7141 caaaggtagt gaagtaagca aacaggcagc tgatatggtt ttaggagatg ataatttcca
     7201 tacgattgca aaagccgtaa aagagggccg gcggattttg gataatttgc aaaaaaccat
     7261 caatttcttc ttgccaactg cattagctca aggattgatc ttgatttggg cacttatgct
     7321 gaatcgcccg ctacctttgt ccccagtaca gattttatgg gtcaatatgg taacgacaat
     7381 cactttatct tatgcactag ggttcgagtc gccagatcca gaaatcatga agcgcccacc
     7441 aagagatcca aagcaaggaa tcctatctgg ttatcatttg tttcggatca tctatgtctc
     7501 cctactgatc atggttcctg cctatttatt ggctatgcag tttgaagggc aaagcttaca
     7561 gcaaaccatt cttctgcaaa atatcgtctt agctcaagct gtttacatga tcaattgcag
     7621 agagttgtcc aagccgtcat tgaaccaagg tcttttgcaa aacaaagcgc tttttctttc
     7681 attaggtatc cttgctttat tgcaagggat ggttattttt ctaccagtcg gccagcagct
     7741 gatcggtaca gtaagtttaa cgatgacaca acagttattg attggagcaa atgtgattct
     7801 tctattctta gttgtagaaa tagaaaaagg gatcatgaac aaactgtttc gcaaaaaaga
     7861 aaaaacagct agagaataca gtcgctagcc gttactataa aaaagttttg aaagtgagtt
     7921 cagtcaaacg attgagctca tttttcgttg gaatagaaga aatgtataca atagggatat
     7981 ctagagaatc tagagggaaa ttggaaatca agatatctgt tttttcaaaa gaaatagtgg
     8041 aaagatcggt tagttcgata ggtaagaagc agacacgttt tcctaagtaa tcttttaatt
     8101 cctgctgcaa gaacgccttc catgaaggtt ctccttggaa aataaaatag acattgaaca
     8161 aattcggttt gatagaaaaa atcgtttgtt ggatcagtag agacaagagt tggagaaagt
     8221 aatttggttc attgatttct atttcttttt ttagatcgtt cagtaaattc tctttctgtt
     8281 gatacaagtg tgcatattgc tgatcatatg tttctaaaaa acgatggtat tgacggtcca
     8341 atgcttcaat caatatcggc gattcatgat attttaatat gaggagagcc aagttatcag
     8401 tcagagatgt tttttttagt tgatcaagag acagctctga agctagttcc tctacaaacg
     8461 tttcgaggac ctctaaaaat ggagaatgat gcaggcagcg agcggtttct tgtccatagt
     8521 tattgttgta attccaactt tccagaaagc agaaagaagc aaaagaaagc tcagtgtctt
     8581 taagataaat accttccgtt aaatacaatt ttttcaatgc agagtcatac agggaggaga
     8641 gtgtgctgtt ggtatggata gaagaatcta atcggcaatt cgcttttata cggatcgtat
     8701 tgataaagaa ccaacatgtt ccgaaaatct cttctgtttc aaccgctagt tcagtttgac
     8761 tgaggtttct taaaaattga aaataatggg attcatggat cgaataatcc tcgaaaaaat
     8821 agttgttatg agtgaaagga atcagcaatc gatgataaaa agtacgaatc gcacgctctt
     8881 ttccaagaat acaagctgta tatttcgtca aatcgatctg caagtcgtaa cttgctaaac
     8941 gctgattgat tttagtcaca tgtcttttca atgtcccata agaaagccca tggtcggcta
     9001 gaaaatcagg gacagcaact ttttttgtaa aaagcagatt tttcagcagt tcgacactga
     9061 ctgattgttt gaaaaaaatc tcccaaaggt catttgttaa taaattttgc tggttttgca
     9121 aactgattcc tgcttgatcg ctgatgatgt cccagccttc gggcagctga gagcgaatag
     9181 attgaaggtc agaaaaaatc gttcgttcgg ttgtccctat gatttcagct aattcattta
     9241 cggttatctt tgtatgattc agcaattgtt ctactaaaga aacatgacgt cgaatatctt
     9301 tttcggtaat gatttttttc attagtgaat acatcaatca gactcccttc tattctaaga
     9361 atacggagaa aataaacggt ggtcaagttt caactgattt ttcaatcatt gtttattttt
     9421 ctggagcatt tactgatgat aagaatttca tcatgaactt gaaaaatgat accactaaaa
     9481 tcaaaaaata ggtgggcaaa caataataaa cattttaata gtagatggta gcaagaccaa
     9541 cacaggcgga ttgaacattt aagagttgat aaaaaagaaa aatatcgaag tatttgatta
     9601 gcaactactt gtattcattt ttctattctt acgtggtatc tttgaataaa gcgaatcaat
     9661 aagaaagaga tggaagcaga tgctaacgaa aaaagaacta atcgatcaca tcaatgaaac
     9721 atacaatttg tctccagaat atatttttaa aaaatttcct gattattgcg tattcagaca
     9781 tcaaaaaagc aaaaaatggt ttggggtcct gatgtctgtt ccagaagaaa aaatctatga
     9841 tgatgcaagt gaccatagga tcatcaatat tcttgatttg aaggtcgatc ctgaacttgg
     9901 cggaattttg cgaggaaaag aggggtattt tccagcctat cacatgaata aagaacattg
     9961 gatctctatt gatctacgta aaatagaaaa gctagatgaa ctagcagatt tgattgatgg
    10021 aagttttaaa tcaacgcaat gaatgtctag gacaatagag aagaggttgt gacagaaatg
    10081 tttagcttca agaaataaga aggaatttac gaaaattgct tctcaaattt ttgggaaatt
    10141 tcggcttatt ttctaagaaa ttacttctgg aacaccgttt gttcgaaaaa agagctgcga
    10201 caagaatttt gtcccagctc tttttttttt attttcctaa ataaaattcc cgcatcgttt
    10261 ttgcaatcat tttgatcaaa ggatgatctt ctgtgaagct agaagataaa taaattgttc
    10321 gtgtcaaggg caaatctttg atttcccgca agacgaaagg atcattttta tcgtaagacc
    10381 aagaaacttt tgggaaaaag ctaacagcta atccttgctt gacaatagaa cgtaatgtcg
    10441 caggatcatc tgtcgtcgaa ctgatcgtca attcgatact tttcgcttca agtgctgcat
    10501 ccagtgtatg acggaaggcg ttgctctttg acagcatcaa gatgggggtg cgttggatat
    10561 cttctaattg cagatgttct tttttagcaa gaggatgttt cttaggaatg gcgacataaa
    10621 caggttcttt catgatagga atattggtca tgccaggctt catctccgaa gtgatgtaca
    10681 gtacaggatc ggtaatatca tctggtaaat gatgttgcat gatcgttaat tggatatcag
    10741 gatgcttttc cataaaaatt cggaccaagt tcggaataga aacagaggct acctctaaat
    10801 acaatatgac ggaagtaacg atcttctcat tgaccgaaga caataatttg attccttctt
    10861 ctaatgtgtc taatgtcttg actgcatatg gataaaattc ttctgcataa acggtcgtct
    10921 caatgtttct gccgctatgg taaaacaaag ggacacctaa ttcgttttcc aaatgtttga
    10981 tcgcacggct caaggcaggt tgagaaataa ataattcttt ggctgcttgg gtaacatttt
    11041 ttgtttcgaa tactttttta aaatactgca gatgaagcaa tttcataatt atccatccca
    11101 tccatacctt tttgttatgg ttttttcttt ttttggcatt ttttattata gcagaaattg
    11161 actaagatag cagtggaaaa agtaagtgct gaggaggcga ggctcatgag tacaggtttc
    11221 ttatttcctg gacaaggcag tcagaagtta gggatgttag cggatatccc tgaaatgtac
    11281 atggaacgtt tagaaaatgt gacggggtat cggttagttg ataaagaaga aaactatcaa
    11341 gatacggttt ttatccagct agcattactg acaaaagcag tattttattt agatgaaatg
    11401 gaaaaaaacg gtattcagcc tgatattgca gcaggacatt cgattggtgc tttttgtgca
    11461 gctgttgctt gtcgatcttt gagatttgag aatgcagcgg aactagtcta tcaccgcgca
    11521 gtcttaatga aagaagctta tccaagcggt tatgcaatgg gcgtagtagt aggtgtgacg
    11581 agaagcgaag ctgaggaaat tgtagcaaaa acatttgatc ctgaccatcc agtttatctg
    11641 tcaaatgaaa attgtccgat gcaacatacg ttatctggga gtatcgaagg tttatccgtt
    11701 acgttggcag aagccaaacg gcaccaagcg caaacagcga agctgctgaa agtccctgtg
    11761 ccatctcact gtctcttgat gaatgatacg gtcaagaaat tcaagccgta tatgcagtct
    11821 gtccatctat ctgatgctag atgtttgtat ataaaaaata cggatggaag agccactcag
    11881 gacgcagaag agattcgtaa agatttgttg gaaaacctag ctttccctgt gcagtggaac
    11941 caaatgatgg acttggcaaa agaattagga atggatctga cgattgaatt tccccccggc
    12001 aatactttga caaagctgat tcatacaaaa tttggtgtag ataaagggat ccgtacgatc
    12061 aatctagacc aacatggtat tgatgacgcc acgtttctat atcaaaaatg gagatgacta
    12121 attatggaaa aaccaaaccg caactgggct tctaaacgcg aaaaaaaagc gcagcgattg
    12181 gaaaaaatca gcaacttgct caatggcaag tatgtagaaa ctgaaaaaat cgtagaagta
    12241 atggaaaaac tgattgctcc cggcgacaaa gttgtattag aaggcgacaa tcaaaaacaa
    12301 gcaagttttc tttcagaaag tcttgaaaaa gtagatgcag aaaaagtgaa tgatctgcat
    12361 ttgatcatgt ctagtatctc acgaccagaa catttgaata tttttgaaaa aggaatcgca
    12421 agcaagatcg atttctctta tgctggtgca caaagcttgc gtgtcgccca aatgattgaa
    12481 gacggcacaa tgaaattagg tgatatccat acttatttgg aactttatgg acgcttattc
    12541 atcgatttga ttccaaatgt tgttttagta gctgcagata aagcagataa agacggcaac
    12601 ttgtacacag gtttcaatac ggaagaaaca ccaacaatca tcgaagctgc agctttcaaa
    12661 gatggtatcg tgatcgttca agttaacgaa gttgtcgatt ctttaccacg tgtagatatc
    12721 cctggtgact gggtagacgt tgtcgtgaaa gcagataaac cttatcaatt agaagcttta
    12781 ttcacacgag atcctcaaaa catcacagaa ttgcaaattc taatggcaat gatggcaatc
    12841 aaaggaatct acgcagaaca cggcgtacaa tctttgaatc acggtattgg attcaataca
    12901 gcagctatcg aattgttact gccaacttat ggtgaatcac tagggttaaa agggaaaatc
    12961 gcgaagaact gggtattgaa cccacatcca acgatgattc cagccatcga atcaggttgg
    13021 gttgaaagca tccatagttt tggtggcgaa gttggtatgg aaaaatatat tgctgctcgc
    13081 ccagatgtct tttttgtcgg taaagacggc acgatgcgca gtaaccgaac attatcacaa
    13141 gctgcaggac aatatgccat cgatatgttt atcggttcaa ccttgcaatt agattacgaa
    13201 ggaaactctt ctaccgtaac aaaaggacgt ttatctggtt ttggcggtgc gcctaacatg
    13261 ggacataatc ctggcggtcg tcgtcattct acaccagcat ggcaatcact tcgcgacaat
    13321 gatgatccat tagggaaagg ccaaaaatta gttgttcaaa tggttgaaac atatggttca
    13381 aataaaaaac cagtctttgt cgaagaatta gatgcactag ctgttcaaaa acaagcaggc
    13441 ttagccaatg caatgcacca gtgatgattt atagcgaaga tactacacat atcgtgacag
    13501 aagaaggaat tgcttatttg tacaaagcaa aaacaaaaga agaacgacaa gcagcaattg
    13561 cagcaattgg cggtgttaca cctcttggaa tgactagcac gaaagaatca ctagatagct
    13621 tgcgtgcagc cggcatcgtg aaattaccag aagatctagg catcaaacgt agtgaagcga
    13681 aacgttcatt attagctgca caaaccatcg atgatttgat cgaatggtca gatggcctgt
    13741 atgaaccacc aatgaaattc agaacttggt cataaaattg gcgagaaaaa ccttttgtcg
    13801 ccatttaccc agatggaaga agaaagacga ataagcagaa cgtgtatgct ctgttttaca
    13861 aaaaggggag agatccatgg aacaattagc ttataaatat ccaacgaaac agccgatcaa
    13921 taagaaaatc catgtaggag tggtcggttc aggagatttg gaaatcttga tgtttccaac
    13981 agaaaaaaca gaatctactg taaaaatttg tacaggaagc gatggcttcg gcgaagtatg
    14041 gaacaatgta ttaacgagat tttttgatcg ttatccaatt tcagcagata tcgtgatcaa
    14101 tgacttcggt gctacaccag gagttgtgta cctacgattg acgcaagcaa tggaggaact
    14161 ttcagatgaa aaatagtttt gtagaattaa acgccagaga acgagtgaac gctctacttg
    14221 acaatggacg tgaattgatt gatccattcg atcaaatcat cgcgccaagt ttaccagcac
    14281 aaggaatcgt ccctgaaagt gatgacggga tcgttgtcag ccgtggcacg atcaatggca
    14341 aacaagcagt agtaatcgct atggaaggaa aattccaagg tggcggtatc ggtgaagttt
    14401 ccggtgcaaa aatcgttgct gcacttgaac atgtgttaga agaaaataaa aaaggaaatg
    14461 agatctttcc agtcattgta ctagatacag gtggtgttcg tttgcaagaa gcaaactacg
    14521 gactgttatc tatttcagaa atcggaaatg taatcattgc tctaaaacaa tatgtaccag
    14581 tcatcggttt gattccagga cgtgtaggat catttggcgg tatgtcgatt acttcagcat
    14641 taatgagtta tttaatcgca acaaaaaaag cgagagtcgg tttgaatgga ccagaagtta
    14701 ttgaacaaga agcaggcgtt cgagaatttg attctagtga caaagacttg atttggaata
    14761 cattaggatc tcgtcaacgg gtagaatcaa aaatcattga tgaattagtg actgatagtg
    14821 tagaagcaat caaagctgct gtcatcgcag caatggacga acgaaaagac acgcatcgca
    14881 gtgaaagctc agcattttat ttatcattac ttgattcttt agatctttca aaaccaatgg
    14941 ccattgacga ttacaatgaa gcaattgcta accatcaagc aaaatcaatt gacttaccag
    15001 aagtagcaga aggaaatgaa ccagctgcag caagtgttgg ttacgattgg tttgcggcac
    15061 taactggcat gaaaaatcca acttcttcta ttagtactgt gtattatggt aaatcaagta
    15121 aattcataga agaagccatc gtgacatcaa tcgtacctga tcctaaaaac ccgatgtacc
    15181 gtgtacgaaa cggtgaagtt ggtttagttg aaggtttccg catgggacaa attttggatc
    15241 acgtttacga agaagataaa gacaaaaaag tgaaacggcc aatcatcgtt gtgatcgatg
    15301 taccaagcca agcatacggc tataacgaag aattgatcgg tatccatgtg gctttggcta
    15361 atagtgctgc tgcttatgca aaactccgtc aagctggaca tccagttatt ggattgattg
    15421 caggaaatgc tatttcaggt gcatttttag cacatggatt acaatcaagc cgcttgattg
    15481 ctttaaacaa taaagaaatt accgtccaag cgatgtctaa agaaagtgct gcacgtgtga
    15541 caaaacgttc ggttgctgaa atcgaagcag ctgctgaaaa agttccagct atcgcttacg
    15601 atatcaataa ttatacaaaa ctaggagcga tctatcattt cctagaagat atcaccgacc
    15661 aagctgcttc agaacaaaat gtcagcaaag tcatcgaagc aatcaatgaa gccattcaag
    15721 atgtccgcaa tacaaatgac acgatgctga caaatcgata cacaaacgaa acagccaaaa
    15781 cattcggacg tgtcgaaaca aataaagtcc gtgccaaaat ggacgaagaa tgggatgcat
    15841 aaaaaatgat tcaagcacat gatattgtcc tttttgataa acatgaaata gaagggttca
    15901 ccttgcctga atgggtcgtt ctaactgaaa agatgttcgg aacagttcgt cgtgcgtcga
    15961 cggatcacgc agaaatacta ccgattggtt tacgtggacc tgcccgcaat cagagatggt
    16021 caacagaaat ctcaaaacat gccgtcaaac aagtcattca tccgtgggac atcatcgaac
    16081 aagaatcgta tagacaagag aaaatcatgg attatcctgt ttatcagcaa tttcatgctg
    16141 ctagaaacat attatcagga tgtaaatggg gaatcggcgg cagcttagga tttgaattat
    16201 cgactgggat tccggcagta aaagaaacca gtgattttga tttgctgctg tacgcaaatt
    16261 caccgattga attgccaatt caggtaattc aatcgcatcc tgcttttttt gagcaattcg
    16321 atacccaagt agtgactcgg caaggcggtt tttcactaaa agagtatttg agagagccag
    16381 agaaaaaaat tctattgaaa acagtcgaag gacctaaatt aaccaaagaa ctttggtaat
    16441 aaagaaaaag agagagaaag agatgtttga aaacaaaaaa atcaaaatca acgtaagtga
    16501 cttccagaaa gtatcagctg taacggctgt tatgatgcaa acgatattga gctttgcttt
    16561 ggcaaacagc cacagcgcaa caaatacagc ttttcttggt accttttacg tcatcgttaa
    16621 atacacagca ccaatgttta tttttgcaat tgtttataat atggtaaaaa caagtcagca
    16681 tttgtcttat tgggaatttt tgaaagaaaa gttttttgaa ctagtcgttc cgtatcttct
    16741 atggacgacg gcttatcttt ggctatttcc agcagtccaa caacaaactc cgtatacgga
    16801 tactgcaagc tttttactga aatatgtcac aggtgatgga gcgcctcatt tatggtatac
    16861 cgttatgatg ctgcagatcc aattgttcat gccgtttttt gtttggctcg gttacaaagt
    16921 atttgctaat aaaaaatatg tatggccagt attgattggt tcaacgattt tatatgtcgc
    16981 atggtatctg ttctatcaaa cacaagtatt gactgggcct tatcatgaca gctggtactt
    17041 attagatcgg tttgttgcta gtttcatgat ttatggggta gccgcatttg tttatcacga
    17101 aaaagtctat caatatttgg acagagtacg ttatcttttc ttacctgttg ccttagttat
    17161 cgcgttcttt tctgtccgtt cattgctcgc acatccagga gacttgagct ttgcgaatgc
    17221 accttacttg aatacgatcc aaagtttata cagtttagtc atcatctttg ctgtcttcat
    17281 aggcgcttcc aaaatgattg taaacgattc gccaaaattg ccattgttca aatggttgtc
    17341 tgtttatgct tatcgtacgt atttagccaa tgtatttgtt ttccaagtac tgttattatt
    17401 gtttaaagat agttggctgc agttaccttc aggagtcatg attcttgtag cttatctaat
    17461 gacggcaagt tgtgcatttg ctttaagctg gttacttcat atcatttggg tagctatcaa
    17521 aaaaggtttc agcaagtaat agtaggaaga aggagtgagt gtcatgataa atgacgaacg
    17581 aaaaaaagca gtgactcagc aactcgctgc gttggtagag gaagcattga tcgcagaagt
    17641 aacattaagt ccaaaacctg gattagtgga tgcactggat gcgggtgcac attccgatat
    17701 ggactatgct ttatttctta agtctgctaa gacgctaact cctttttttg aagaaatggc
    17761 tcaaatagca tggcatcatg ctattgatca ggagttaaga gaaagaattg ctgagattgg
    17821 gcgaaaagcg gaaaaggcaa tgctgattgc tacaaatggc atcaataccc ataaaggagc
    17881 gatttgggca atggggctat tgatcagtgt cattgcacaa aaaatcagta tggaacagcc
    17941 tttttcttta tcgaaaatac ttgatgatgt cagcaagctt gctgcttttc ctgatacaaa
    18001 atatcgtcat aaaaaaacca ctcatggtca tagggtgaag caaaaatatg gcgtaaatgg
    18061 tgcatatgaa gaagcgatat tagggtatcc gcatatcagg cttgcaattc aagcagctaa
    18121 ggagctagat aatgattcat atgtacagaa acaaatgcat atgttgttag ttttgatgag
    18181 ttcgctagat gacacatgcg tattgcatcg cagtgatcag aaaacgttag tggaaatgca
    18241 aaaacttgct catgctgcta gtcaaagtgc tttaccaaat cgtccattta cagcactgtt
    18301 gcacttttgt caggaaaacc aaatctctcc tggaggaagc gcagatctct tgtctgcaag
    18361 cttgttcatg ctgaagatag aggaattatg gactgccgac acatacattt gtccgatgtt
    18421 actcaatgac taaataagac aaaattgcta aaaaaacagt ttatgcatct ggttattgac
    18481 tcttcctttt agcaaggcta tcataaaaat agttttttgt aataaaagat aacgaggaag
    18541 acaatgaaaa aaatcaatta tttagcaatc attttgttag tgggcctatg tttgtttgct
    18601 ctgccgaccc aaggattcgc tgtcgcgggt ggtcatggtg gaggtacatc tagcggtcga
    18661 agttcgggca gaggttcatt tggtggtcgt tcatatgggg gcagttattt tggcagtcgt
    18721 tcttacggca gagattctta tgggtattat ggtccaggta caggtccttc tagttatgag
    18781 caaacgggat ttcttgcatt agctgttttg tcagtaggag gggtttctgc tttttctatc
    18841 gtcaagaata aaagacaaaa gaaaaggctg aaagcggaga tattgtcccg catgtcagga
    18901 acggctaaag aaaagaaagc acagtttctg gaaatccaaa agatattcat gaagatccaa
    18961 tccgcttggg aagaagaaac tcctgaaaaa gccagaagct gttatacaga caagctgttt
    19021 gccgaacatc agaaagttct gcaaaaaaac aaagcagacg gtgtaagaaa tcatactaaa
    19081 aaaataacta ttcaggaagt aggaaattat cgacagatcc acgaaaatag ttttgccatc
    19141 cgaattgatt tcagatgctg tgattattca gtcgatcgaa aagatgggcg attgatctca
    19201 ggaagtaaga cacaacgaca gtatttttct cagatatggt attttgattt ttctgagaaa
    19261 gaaaaaagat ggcaagccga ttttattcag ccgattatgc tggattgaca ggaaatattt
    19321 atgaacggag ttgtgacaaa agccttgtcg cagctccttt ttctgaataa acggtgtgcc
    19381 agagatagcc tatcggcaat aaggcacgat tttgtaaaat atgggaagcg atttttcaaa
    19441 atcgcttctt atcgctcaag gctgaacatc tctgtcacaa cctctccact aaatggtgtt
    19501 taaaagcttg catctgcgat aataagaaca accaaacgaa aaataggaag cactatttta
    19561 gtttagttgt tcttattact tgatgcagga cagctttttc acaacctctt ttttgtatag
    19621 atgggcaaaa tttttgtaat aataggcgta tgtctttttc gttgaggaga tgaaaaagca
    19681 tatggcttat tttttgtttg ctttgcttac tttctttcca cttcaagtgg cagaaatatc
    19741 tactttttat tatgtaagcg gaaccatata cttgaaatca gaaggagatc atttctatga
    19801 aaacaagaac aattgaaata cttcacagaa tcatgacagc ccaatatcca gtaaaagcaa
    19861 aacatctagc aagtgagttc aatgtcagtc agcggacgat ccgtcaggaa gttcttgaag
    19921 cgaatatctg gttgagcagt aaaaagcttc cggagatccg tacaattcgc aacaaaggtt
    19981 ttttgttgaa gctggaaaag gaaaagcaag ctcgattaga aactgaaata aaagatgtga
    20041 aagacggctt tttgaatcgg cgcgaacgta tttttgatct agttttagcc gttgcttaca
    20101 gccgaatccc gatccatcta aacaaaaaag aagaagaatt tcaaatctct aaaagtacat
    20161 tagatgaaga tatgagacgg ttacgagcaa cactggtcag ttatgggata gagatcgtca
    20221 gttatggaaa gcaaggactg gtttataaag gttcagaacg atctatccgt acgatgatct
    20281 atgattttat caatcagaat ctgggaatga ccgacttctt tatgaatcaa gatgcacaat
    20341 taacaccggc tcaaaaaatc tttctccatt attttccttt ggtggaaatc aaaaaaatac
    20401 aggagatcta ttccaataag ttgttgaaac aagaagatga tatttataaa aaacaaatcc
    20461 tgctttttac attgatctgg atccagcgaa tcaggaaaca tgaatttatc tctgccatta
    20521 attggaaaaa tacagaaata aaagacagta gcttttctgc atttattgac cacatgatcg
    20581 aggaatttca attggacaat gtcccgcaag tagaaagaaa ttatcttcgt ttcactattg
    20641 agacgttcaa tgctagagat atctccaatt caatcgagtg ggtacaagct caattactaa
    20701 cgatccaatt gatccaattt gtagagaacg agacacacgt acctttccat ctaaaggaag
    20761 aagctttatt cgaaaatctc tataagcaca tggctgcact gattgtaaga atcaagaatc
    20821 atatccaagt catgaatcca ttgaaagaaa acatccgaac aaattatttc ccaatctacc
    20881 gcgcagtcac tcattttgtt cctacgatcg aagaagtaat tggtggcaag atactggagg
    20941 acgagatagc ttttcttgtc atccactttt cgacgatcgc cagtgtaatc aagcgagact
    21001 tgaattatgt ttataaatca gtcgttgtct gtaaccatgg gatggctact ggaaatctgt
    21061 tagcagagaa tctgaaagaa aagtttcctc agatcgagat tactgcagtt ttaagttcaa
    21121 aagaaatcga attagtagat aaattggatg tcaatctggt tttcagcacc tttcaattga
    21181 attatcgaaa taagccgctt ttagttgtcg atccgatttt aactgagacg aatatcttgg
    21241 ttatagaaga ctttttgaaa agaaatgaag aactacagcg gttagagcct aacagtcatg
    21301 aatcgacgga gttattcact caaataatag atgtcatgaa agaaagtggt ggccgagtag
    21361 atcgaatcat ctatggcaag ttagaaaaat tattcgaaac aaacaattta gaaatcaaca
    21421 aaagggagat ccagcctatg ttaaaagata ttttgacgga cagcaatatc atgatcaaag
    21481 aaaaagccac tacttgggag gaatcgattg aactagctgc tagaccattg gttaaagaaa
    21541 acattgtcga agagcgctat gtcgaagcga tgattcgtgc agtgaatgat tacggaccgt
    21601 atatcgtgat tggcaaacac atggcactag cccatgcccg accagaagat ggggtcaata
    21661 aattaggagt cagcgtagct acgattgagc aaccaatcga tttcggtaat cctgaaatgg
    21721 atccagtaaa gattattttt tgtttggcag cagtagactc ctactcgcat ctgaacatca
    21781 tgaaagaact aattgaattg atcaacaacg aagagaagtt ggatcgattg attgaatgca
    21841 caaatatcga agatttcaaa caactgcttt tttcagtagt tgagaaataa taaatgatac
    21901 gggggagaac aaagatgaac aaattgaaca ttttattcgt atgcggtgca ggtttaggaa
    21961 gtagttttgc agcacagatg tctgctgagg atgttttgaa caaattagga gtagaagcca
    22021 aactggacca ttgtgatatt tcttccgcag tttccatgaa accagatgtg atcatcacag
    22081 cagaaaattt tcgttcgcag ttcgaaaagt tcgcagttga tcctaaaacg gcgatgatct
    22141 atttgaaaaa tattgtttca aaaaatgaaa ttgaagaaaa aatcacccca gttctgcaag
    22201 ataagggagt tatctaaata caaaaagaaa agagggtaaa aa
//
LOCUS       DAYYHS010000047        21685 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000047 DAYYHS010000000
VERSION     DAYYHS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.047"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(402..1898)
                     /gene="lysS"
                     /locus_tag="ACHA6A_001725"
     CDS             complement(402..1898)
                     /gene="lysS"
                     /locus_tag="ACHA6A_001725"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8969916.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2009..3013)
                     /gene="dusB"
                     /locus_tag="ACHA6A_001726"
     CDS             complement(2009..3013)
                     /gene="dusB"
                     /locus_tag="ACHA6A_001726"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8969917.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3014..3901)
                     /gene="hslO"
                     /locus_tag="ACHA6A_001727"
     CDS             complement(3014..3901)
                     /gene="hslO"
                     /locus_tag="ACHA6A_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8969918.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4118..6229)
                     /gene="ftsH"
                     /locus_tag="ACHA6A_001728"
     CDS             complement(4118..6229)
                     /gene="ftsH"
                     /locus_tag="ACHA6A_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8969919.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6319..6864)
                     /gene="hpt"
                     /locus_tag="ACHA6A_001729"
     CDS             complement(6319..6864)
                     /gene="hpt"
                     /locus_tag="ACHA6A_001729"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8969920.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6864..8309)
                     /gene="tilS"
                     /locus_tag="ACHA6A_001730"
     CDS             complement(6864..8309)
                     /gene="tilS"
                     /locus_tag="ACHA6A_001730"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8969921.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8429..8899)
                     /locus_tag="ACHA6A_001731"
     CDS             complement(8429..8899)
                     /locus_tag="ACHA6A_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8969922.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8945..9370)
                     /locus_tag="ACHA6A_001732"
     CDS             complement(8945..9370)
                     /locus_tag="ACHA6A_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8969923.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9437..9706)
                     /locus_tag="ACHA6A_001733"
     CDS             complement(9437..9706)
                     /locus_tag="ACHA6A_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8969924.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9717..11312)
                     /locus_tag="ACHA6A_001734"
     CDS             complement(9717..11312)
                     /locus_tag="ACHA6A_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8969925.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11327..14848)
                     /gene="mfd"
                     /locus_tag="ACHA6A_001735"
     CDS             complement(11327..14848)
                     /gene="mfd"
                     /locus_tag="ACHA6A_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8969926.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14865..15425)
                     /gene="pth"
                     /locus_tag="ACHA6A_001736"
     CDS             complement(14865..15425)
                     /gene="pth"
                     /locus_tag="ACHA6A_001736"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8969927.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15778..16752
                     /locus_tag="ACHA6A_001737"
     CDS             15778..16752
                     /locus_tag="ACHA6A_001737"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8969928.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16922..18322
                     /locus_tag="ACHA6A_001738"
     CDS             16922..18322
                     /locus_tag="ACHA6A_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8969929.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18400..19122)
                     /locus_tag="ACHA6A_001739"
     CDS             complement(18400..19122)
                     /locus_tag="ACHA6A_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8969930.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19201..19707)
                     /locus_tag="ACHA6A_001740"
     CDS             complement(19201..19707)
                     /locus_tag="ACHA6A_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8969931.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19830..21629
                     /locus_tag="ACHA6A_001741"
     CDS             19830..21629
                     /locus_tag="ACHA6A_001741"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8969932.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6023 a         4728 c         3890 g         7044 t
ORIGIN      
        1 tatcataact tatcgtttgt tatttgtcaa gaactttttt gaagtttttt tcgatagaat
       61 caagcttcgc aagtggttct tttttaacga ctcagttatc ttatcatgtc gttagtaaca
      121 tgtcaataac ttttttgtta tgtttttgaa taaatatttt cttctgtctt ttggacaagt
      181 aataatatac caactaaatt tagcttggtc aacgaatttt ttgtattttt tcaagttttt
      241 ttcaataaac gattattcat aacttttctt tctttatcgt tcgttttcgt cttctttaat
      301 gtctagaata gtcggcatta gcttttgaac tttcgttatt atttattcct ttctacacag
      361 caaaagctgc ctaaggaatt ccttagacag cttttattcg attatctcat tgttgggaat
      421 agcaaaacat cccgaattga ttgtgcatca gttagcaaca ttactaatct gtcgatcccg
      481 atgcctagac caccagttgg cggcataccg tattctaagg cttccaagaa gtcttcgtct
      541 acaccgtgag cttcgtcatt tcctagttca cgttcttttt cttgcgcttc gaatctttct
      601 ctttgatcaa ttggatcgtt cagttctgta aaggcattag caaattcgcg ccccactatg
      661 aatagttcga atcgatctgt gaatcgagga tcttctggat ttttcttcgc taaaggcgaa
      721 acttcgactg gatgtccgta tacaaacgtc ggttgtgtca atgtttcttc aacaaatgtt
      781 tcaaagaatt cattgataac atgtccgaca cccatagctt cggttacttc tacattgtgt
      841 ttttcggcta aagctaatgc ttcttccaat gtcatttctt gccagaagtc tacaccagtt
      901 tgttctttga ttgcatctac catatggatg cgtttaaagt cactttctaa gtcgacagct
      961 tgtccatcat atgtgatttt tgctgttcct agtacttttt cagctgcgtt acggataata
     1021 ccttctgtca gattcattac atcattgaaa tcagtatatg ctgtgtatgc ttctaacatc
     1081 gtaaattctg gattatgtgt agtatcaatt ccttcattac ggaaaacgcg gccgatttca
     1141 taaacttttt ccattccgcc aacaattagt cgttttaaat gaagttctag cgcaatacgc
     1201 aaatataaat caatgtccag tgcattatga tgcgtgataa atgggcgtgc ggcagctcca
     1261 cctgcctcat tgtgcaatac tggtgtttct acttcaatat agccattgcc atctaaatag
     1321 cgacggattt cgctgataat ttggcttcgt ttcataaatc ggtcaaagct ttcgcgatta
     1381 ctgattaaat ccaagtagcg ttgtctgtag cgttgttcaa tattcgttaa gccatggtat
     1441 ttatctggca gtggacgaag tgcttttgac aaaatcgtga tttctgaagc tttgatagaa
     1501 acttcgcctg tgtctgtttt catgatttgt ccagttacac caaagaaatc tccaaggtct
     1561 gcatgtttga atacttcata ggcctcatca cctacttggt cttttcttac ataaatttga
     1621 atttggcctt cacgatcttg tagatgcgca aatccagctt tcccttttcc tcggcgagtc
     1681 atcatacgac cagctacact agcggtcaat cccatttctg ctagttcttc ttttgttctt
     1741 ggatcaaata gttcatgcaa ttcttctgag ttgtgcgtgc gttcaaatcg ttttccaaat
     1801 ggatcgattc catcttcacg caattgttcc attttttgac gacgtacaag catttgatca
     1861 tttaaatctt ctacttgatg ttgttgttcc tgtgtcacaa aagttcctcc attctcatgt
     1921 taatctcttt cctataatgc caatgaaaca caaaaaaagc aagccgaact tggaatttcg
     1981 gcttgaaaat tcacgaaaaa cttagaaatc acaacgtttg tttcaatagt actttttccg
     2041 ctttttctac aaattgatcc agcaggtcat tgatttcttg ctgtttttct gtctgattga
     2101 ttgctacttt cactttcgct gctcgaggaa tcccttttaa ataataagcc gcatgtgttc
     2161 tgaattctct acaagctact ttttctcctt tgagatctgc taagcgttgt aaatgaagtt
     2221 tagctgtagc aattttttcg gctggtgttg gttcaggaat cagttcccct gtttcgagat
     2281 aatgctgtgt gcggtgaatc atccatggat tgcccagcgc tgcacgacca atcatcacac
     2341 cgtctgcacc gacatagtca agcatccatt ttgcatcttc tggcgttttg acatccccgt
     2401 tccccataaa tggaatcgtc aaatgttttt tcacttcttt caatacttcc cagtccgctt
     2461 ttccttcata catctgaacc cgtgtacgtc catgcatagc gatggcactt gctccagctg
     2521 attgtgctgc taatgcattt tctacagcaa agacgtgctc ttcatcccaa ccaatacgca
     2581 tcttgattgt tactgggata tttactgctg aagaaacagc ttggaccatt tcgtatactt
     2641 tctcaggatc taaaagccat tttgctcctg cttctgcttt gattacttta tttactggac
     2701 agcccatatt gatatctatt atatcggcag ctgtgttttc ttcgacaaat tgagcagctt
     2761 caactagact atctttattt ccgccaaata tctgtaaact taatggatgt tctgtttctt
     2821 caatgtggag catctctaaa gtttttttgt ttctgaaatg aataccttgg tcactgatca
     2881 tttcacatac gactaagccc gcaccaaatt ctttgacagt gactcgaaat gctgaatttg
     2941 ttattccagc catcggtgca acaacgacac gattagggat ttccacattg ccaattttcc
     3001 atgtgtgttc cacttaaatc cctcctaagg cttctttctt caatccatct aattcttctt
     3061 cgctatactt gtatttgtta ttacagaaag aacaaaccgc ttctgcaccg tgatcttcgt
     3121 ctatcattgc ttggatttct tctgcaccta acgcgataat tgcttctgca aatttttctt
     3181 ttgaacagtc acatttgaat tgaacaggca ttttttccaa aatgttcaat tcttctgtac
     3241 caagcaatcc tttcaatatt tcttctggtg tctgtccttc atctaagagg gtagagattc
     3301 ttggtgtcgt ttttaattgt tcttcgatct tgctgattgt ttcttcatcg gcacctggca
     3361 tgatttggat catgaatcct cccgccgtct tgatgctgtc gtcagtattg accaatacac
     3421 tcagtccaac agctgaaggg atttgttctg agatagcaag atagtaagta aaatcttcag
     3481 cgatttctcc agaaacaatc ggcgtttgcc cagaaaaagg ttcttttaat cctaagtctt
     3541 ttgtgacagt aaacattcct tgtgttccaa ctgctccccg cacatcgatt ttaccttcac
     3601 tatttagagg aagactgaga tgcggatttt tgatgtaacc tttcacatct ccctttccat
     3661 ttgcatctac tacaattcct gttgctggac catcgccttg gattcgaaca gttagtttgt
     3721 cttctccttt gagcgtcgca ccaagtagca gactaccgac caatgtccgt cccaaagcag
     3781 ctgaggaggt gtgccaagta tcatgacgtc tttgtgcctc cgctacggta tttgttgctt
     3841 gaacggcgta tgctcggacg aatccgtcgt gagcaagtgc cttaaccaaa taatctttca
     3901 tttgttatgt actccttttt ccgtttttaa actcatagtg aatcatacta gcaactactg
     3961 cttttttcaa ctttggaatg cttcttttaa aaataattca gaatcatcta gaaaacagga
     4021 catcacccta tttacaactt aaataaagaa atctcccgaa aaccggtttt tgttccggtt
     4081 ttcgggagtc atatcagatc aattgaccta tattttttta tttgtattga tcgtctgaat
     4141 gattatcttc tgattgttga gaatctgaat catgttctgc tttattttcc tcattcagtt
     4201 catgttttgc ttcttcaaaa tcacgttttt cttccgcttg tttttcagcg tctttttctt
     4261 ccaaagcacg ttttgcttct tcaaaagttt tcgcttcttt ttcgcttgga taatcatttg
     4321 actcagtacc ttcaggcatc acaccgtgtt caaacaatga cttgattgct ttggcatcca
     4381 atgtttcgaa ttccaaaagt ttttcagcaa tcagtttatg ctgtgcacga tgagcttcga
     4441 tgatttctcg agctttatca tgagcttcca tcaagatttt tcgtacttct tgatcaatct
     4501 cgaaagcgac ttgttccgag taagcttttg tttgaccata gtcgcggcca acaaacactt
     4561 gatggtttcc ttcatattga acaggaccta aacgatcact cattccatat tctgttacca
     4621 tgcttcgtgc tagagctgtc gcttgctcaa agtcattgga agcaccagtt gattggacat
     4681 tgaagatgat ttcttcagct gtacgtccac caagtaagcc tacgatttgt tcaaacatat
     4741 cttctttcgt catcaagaac tgatcttctt ttggtaaagc gatcatatat ccgccggcgc
     4801 gtccgcgagg gatgatcgtt actttgtgga cgacccgagc gcgactcaat actaatccga
     4861 caatcgtatg accagcttcg tgataagcaa ccatttcgcg ttcttttttg ctgattacgc
     4921 gatctttttt agctggacct gcaatcacgc gatcttccgc ttcatcgata tcagaagcat
     4981 cgattttctt tttatttctt ctagcagcta ctaacgctgc ttcattcaag acattttcta
     5041 aatctgctcc ggcaaaacct ggtgtctgtt gagccacaac tttcaagtca acgtcatctg
     5101 ccatcggttt attccgtgca tggactcgca agattgcttc acgacctttt acatctggac
     5161 gtccaactaa aatctggcgg tcaaaacggc ctggacgtag taacgctggg tctagtacat
     5221 cagaacggtt ggttgcagca atgacgatta ctccttcatt tccgtcgaag ccatccattt
     5281 ctacaagcaa ttggttcaat gtttgttcgc gttcatcatg tccgccgccc ataccggcac
     5341 cacgttgacg acctaccgca tcaatttcat caatgaaaat gatagctggt gcgtttttct
     5401 tcgctgtttc aaacaaatca cgaacacgac ttgctccaac accgacaaac atttctacga
     5461 aatcagaacc cgagatcgag taaaacggta ctccagcttc tccggcaaca gcttttgcta
     5521 ataaggtttt acctgttcct ggaggtcctt ctaaaagtac acctgccggg atacgggcac
     5581 ctaattcaac aaatcggcga ggatctttca gaaattcaac aacttcaact agttcttgtt
     5641 tttcttcttc cgcacccgct acatcagaaa aacgtacgcg attagctttt ttgtctgctt
     5701 ctttggcttt tgatttacca aagttcatga cacggccgcc accgccaccg ccgccgcctt
     5761 gttgtcccat catcatgtaa aagaaaaaga tgatgattac gattggcaag aagctaagca
     5821 atagggaaac ccatataccg ctgcttgatt cttcttttac tgttgtagca actttgtttt
     5881 cttttgccag ttctgttact tggctcaatg ttgtatcatt tggcaaaatg attgttgtga
     5941 aatgggtagt agtcgtatct gttgaaccta agatagctaa cccaccgctg ttagcgacgg
     6001 tctgctgttc tttgtattca ccagtgatct tatatacacc atttgcaggc tgcacttcaa
     6061 attctttgat cttgcctgct tgtagttgtt ctgtgaattt tgagtactcg atatctgacg
     6121 atcgctggcc gttattacca aaaatgaaat aaacgaccat taccatcgct aagattacga
     6181 ggacataata caaggcattt ttcatcatgc cgttgttctt tttgttcata gttgccctcc
     6241 ctaagcgtca atttaattta acagtttttt atcaatcctg accattctat tgtaccataa
     6301 tttaattgag atacagcatt aatttgattg ataaacacta ggtttcaata caccgatgta
     6361 cggaaggtta cgatatgctt ctgcataatc caagccataa ccgaccacaa attcatttgg
     6421 tacgttgaag ccaacataat ctgcttcgat atttactact cttccttcag gtttgtcaag
     6481 tagtgtaaca attttgactg attttgcttt gcggtatttg aaaagatcca ctaaataagc
     6541 aagtgttctt ccgctgtcaa tgatatcttc aacgatcaac agatcgcgac cttctacatt
     6601 cgtatctaag tctttgacga tttttacttc ccctgacgag actgttgcat tcccgtagct
     6661 tgaaacatcc atgaaatcca attccaaata cgtatcgata gaacgaacga tatctgccat
     6721 gaatggtacc gctcctttta agacacctac tactagggga ttggtgtttt ggtaaatttc
     6781 tgttaactct ttgcctagtt ctttgcaacg aacttgtatc tcttcttgtg agatcaaaat
     6841 tctctcaata tccttttcca acatcaggtt ctccttccaa ttgcttcttt ttgatatttg
     6901 taaagaagta tgtagtgtat tttatcagtt tcttcacgaa tactcaaata tgaaaaggca
     6961 aaaggtatca agctatatat atttcttttg gcatctgtaa tgatccaagc atgctgcctt
     7021 tgtgaattag gtactttctg atcgataaaa tatctagata ctttttttgt gagattctct
     7081 gttaatttaa tgcggtctcc tgcttttctt ttttcgacat acagttgcac atcagcagga
     7141 atccaaatgt catgagaaaa tagagaccat gaatctgttg ctttgatgtc ccctgcattt
     7201 tcaggtttga ccagaccaat ccattctgtt tcattcaaat atattccttg ttctggaatc
     7261 agcaacaagc gctggtcttc tgtctggcta gttgcttgtt tttctttttt agagagtaca
     7321 acaaaatgat actctctttt cagtatccaa ttattgccaa tactgagttg ccattggctt
     7381 tttttctgtt gccactggtc taaaatcgac tccatttgtt gttctttcaa aataattcct
     7441 gtttttgaga atgctttgtc gaaaaaggag taaaaaaaat agtatcgttc agcatctgtc
     7501 atttcttcaa tcatttctgc agaaaatcga aattgttcct cccactgttc gacttccttt
     7561 tcgatcaact gtcccatata tttttgaatg acttggtctg cccattggat ctgttgcgaa
     7621 aattgctgga aatgtctcaa cgcctgtgta ttttcttgtt tcaattgagg aactaccaaa
     7681 tgacgaagcc gatttctctg gatatctaac aattggtttg tctgatcctc aaagtaaaca
     7741 agctgcgatt ccgctgcata agtatatagg tcttctttgc taaaactcag caacggacga
     7801 atcagtctgc ccgtagcaaa tggttgtact tctttgattc cagaaaaagt atttaactgt
     7861 cccccacgaa cgattttcat taacaccgtc tcgatttgat catctccgtg atgagcagtc
     7921 atcaacgtgt catattgtcc ttcttccatc acgcgagtga attcttgata acgaaagtta
     7981 cgagcttctg tttctatggc ggatttggct ggatcttccc aaatacttac atataaaggg
     8041 agattcttct cttcacaata tgagcgaaga taggcagctt cttctgctga ttcttctctt
     8101 aattggtgat tgatgtggac gatacctaaa gaaaaaccga tttggcgttg cacttgtaaa
     8161 agtaaatgga gcaaaaccat cgaatcaaca ccacctgata ccgctgctaa aattctcgat
     8221 ccttgttgcc agaatccttg ttcattcccg tgccgaataa atgactgctg taatagcggt
     8281 gcttttttcc cgcctttttc aacgttcatc ctttccacac cctttcttcc taaaaaaaag
     8341 agagcgggaa aacgtctaaa acgacaatta acctgccctc tgttgatact atgacccaaa
     8401 tagtcgcact atcattattt gcggcttttt agctgcgacg tccgccacgg ccaccgcgct
     8461 ttccttctgt gtttcgcttc aatgaagata agcggtcatc gctatctttc aaaaatgaac
     8521 tcatcaatga atcgaagtct tctttttgag ctgaaggttg actgcgagaa aaagattttt
     8581 tctgtgctgg acgatttcgg ttgtcacggg gatctttttg ttgatatttt ttaggacggc
     8641 tttcttctcc agattcagca ggttgttcct gcgctttccg gatagatagt ccgatttttc
     8701 catcatcatt tactgaaata actttaactg ttacttcatc tcctacactt aaaacatcat
     8761 ggatgtcttt tacatagcca tttgacactt cgctaatatg aactagaccc gtttttccag
     8821 cacctagatc aataaacgca ccaaaatttg tgattcctga tacttttcct tgcagttttg
     8881 ctcctacctc gattgacata aaaaaaatgt tcctcctaat tatttccctt ttttatattc
     8941 ctgcttattt tgcagttgta gaactagttg attgatctgt ttcatttgtc gagctgtctg
     9001 tatttgattc taatatagga taaacttttt caccgtcttt tgagtaaaag aatttgcttc
     9061 tggcaagttt agctacatac tcatcatctt tcagcaatga aacatcttgg tgcagctgat
     9121 caacgtctgc gttgacttct ttcatttcag tatttgcttc gactctcaat tcgttcaatt
     9181 gatgcatcct ttgcatgtca tggaaaatct gcacacctat tatcgcgaaa atgaccaaag
     9241 caccaacaaa aattgcagcc agtctgcgtc ttcgaaaaat cagttgacgt tgttgttgct
     9301 gaaattcagc atactttttc tttgcatatt ccgtatctag cgcagcgatt ttattgcttt
     9361 ttttcgtcac ttggattcgc tccaatcttt ttgattgtct ttttcattat accaatatcg
     9421 tgaaagcttg tctaatctat ttctctagtg tttctgtgcg attttcagaa ataatttcgt
     9481 acattttttg tgcatcttct tttttggttg attcatgtaa ttccgtaatt ttcacttcca
     9541 acgttttgtt gccaaactgg actttcaaaa tatcgcctac tttgacatca ctggaagatt
     9601 tcgccaattt accgttgatt tgaattcttc ctttatcagc tacttctttt gctactgatc
     9661 gtcttttgat gattcgggaa atttttaaaa atttatctaa tcgcatatct tattcctcac
     9721 tttttaattt ttttatttaa tcgcaggatt ttcttaccaa aaggcaacat tagccattct
     9781 cgtactgtaa acagacgaag acgaatcact gcataaatga acacagaagc cccgatccca
     9841 actcctatca cgctgacaaa aaaagcagtg gaacgatgag ttacaggacc aaatactata
     9901 ttcaatacac cataatacag cagcagtact actgtcatac aaagaaggct gaacatcaac
     9961 ttccgtccaa aatgccgttc tttccaaaaa gcatttagct cttttgtttc aattcgaaca
    10021 aaataccaaa gcgtcacacc taatcctaaa agggtagaca ggcttgcacc tgctgttcct
    10081 aataaaccgg tcagaaaaga agtagtcaat ccttttacca atagtcccca tcctgcgcct
    10141 tttaaagaag ggcgaaacga gttcttgctt tgagcaatac tttgataagc ttggatgact
    10201 gcagtgaaag caatggaaaa aacaaataat acaagcgctg catttcccgc ataatcttta
    10261 aacaacgcat aattgatata agggagcagc aaggctaatc ccaaagacgc agctaatgct
    10321 aaagcggtcg tcaatctaag atagattttg gctgtctgta aaaactgacg atagatccgg
    10381 ttggtcaaat gacgagtcaa cgcaggcagg aatgtcgcac ttaatgcagt ggcaatcact
    10441 aaacctaatt gtactaaagg ctgacctcga tcatagaccc cttttgctat cttcgcaccg
    10501 tattctgaca gtccgtaaac ttccaaagca ttttttacaa agaaagagtc gatcagctgg
    10561 aaaaagataa gcaatccact ataaatcgaa accagtccgc cttctaccag aaaacgccgg
    10621 atcaagggct ttgacggctg tttgaacaat ggaaaccgtc ggaagcttaa cgctcctcca
    10681 tgaatctttt gttcatagta gtgaaggatc ccatatgcgc ataaaccgcc aaaaacagct
    10741 ccactcattg ctgccgtccc tgtttggtac accgtccatc caagctttct aaaagcaaac
    10801 gcactacaga caatcacaaa aacacgaacg aactgctcaa ctacttgaga aacagcagtt
    10861 ggttccatca agaatcttcc ttggaaattc cctcgataaa aagatagacc aggcatcagc
    10921 aaaaaggtaa aggaaactac ttggatcagc ggtttcaatt gtatgtctcc catcatccaa
    10981 gcaatccagc tgctaaatgc aaagacaaac aaccacaaaa agacacctgt ccaaaatact
    11041 aagggataaa gattttgcaa agcttgcttt cgctcgaaag gattctttct ttctgcaacg
    11101 tactttgata tgaactgcgg aagaccggat aaagctaaag tcattgctat cccataaatt
    11161 ggataaactt gctgataaac ataaaaacct tcgtcgccga ctaagttttg gaggggaatt
    11221 cggtaaaggg cacttaatac ttttgctatg aaagaagcaa tcgtcaatac aaatgctcct
    11281 tgcatgacgc gccgcatttc tttatgatcc aaatcattgc tccttcctac gctgacagat
    11341 atttttgctc acgcaacgct ttgacaaact tttggatttc ctgaagccat actgcttctt
    11401 ccatttggtt ggggatcgtc aagcgtatca ccatctgttc tttttcaacg cctagactgg
    11461 cttttagttt tgtcgctgtc agtgcttcga ataactgttc cacactatag cgttttgttc
    11521 caactttact tagagtaaag actatatgcg cctcctgttt ccttatcgtt tctaggagcg
    11581 ctcgatcgcc atccattttg atctgcccaa ttgtcaagag atgtgctact tcttctggat
    11641 attctccaaa acggtcgagc agatcatctt ctagttcatc tagcatttct tgcgaatcaa
    11701 gttcacgaat tcgtttatag atctcgattt tctgccgttg atctgccacg tatgtttcag
    11761 gtagatacgc atcgacaccg aggtcgatct caacagtggt cttctccact tgactattct
    11821 ttccttgttt acgagaaaca gcttcgctta gcatttgaga atacatatcg aaaccgactg
    11881 catcaataaa tccatgctgc tgagcaccta agagattacc tgcaccgcga atggaaagat
    11941 cccgcatcgc aatcttgaat ccagatccca gttctgtaaa atctttgatt gcctgaagac
    12001 gtttttcact gacttcgtta aggatcttct gctgctcata cataaagtaa gcatatgcga
    12061 cacggctgct tcttcctacc cgccctcgca attggtataa cgtggataat cccatataat
    12121 cagcgttctc aacgaacaac gtatttgcgt tcgggatatc tactcctgtt tcaatgatgg
    12181 ttgtagtgac aagaacatca tattgacctt caataaattc aaaaagcgta ttttctaatt
    12241 gcgcctctgt catttgccca tgagcataac cgatccgtgc ttccggaacg agttcttgga
    12301 tttcttctac tttttgttcg atcgtttcta cccgattata taaatagaat acttgtccac
    12361 ctcgtcccat ttctcgatgg attgcttcgc gaatagcccc aggatttttt tccataacat
    12421 acgtttggat cggataacga ttcgcaggcg gtgtttcgat tactgaaaga tcccgcacgc
    12481 ctaacatcga catatgcaaa gttctcggaa tcggtgttgc agtcagagtc aaaacatcca
    12541 cttgtgcacg taactgtttc aatcgttcct tatgttttac accaaaacgt tgttcttcgt
    12601 caatgatcag tagacccaaa tcgctaaatt caatatcttt tgacaaaatt cggtgggttc
    12661 ctacaacaat atcaacttga cctgtacgca gttgttcgat ggtttctttt tgttgctttc
    12721 ttgttctgaa acgacttaat agcccgatat ttacggggaa accttcaaat ctttccagca
    12781 tcgtttcata gtgctgctgt gccaagatcg ttgttggaac taaaaaggct acttgtttgc
    12841 tttcctttat cgctttaaag gccgctcgaa gcgctacttc tgttttccca taaccaacat
    12901 cacctacgag tagtcgatcc attggttttt ctttttccat atcccgtttg atctccgctg
    12961 cacttctcaa ctggtcatca gtttcagagt aggggaaggc attttcgaat tctttttggt
    13021 aggcgtcatc aggtccaaaa gcatatcctt tttctgactc tcttgcggca tataatttga
    13081 ttagatcgtc agcgatatcc tcgatttttg aagagacttt tcgcttggtt ttcgtccatt
    13141 cacttccgcc tagcttattg atgcgaggcg ctttggcttc agaggcgaca tatttttgga
    13201 tcagattcag ttgagtaaca ggaataaaca gtttgtcgtc gttttgatac aaaatagtca
    13261 tatagtcttg gtgaacgcca tctacttcta gcgtttccat cccaatgtat ttcccgatac
    13321 catgattggc atggacaacg taatctcctg cttttaactc attatagctt ttcaatcgct
    13381 ctgcgtttgt tactgtttga cgtctcgttt gttttttcgt cgttttttgg aaaatttctt
    13441 tttctgtgat agctactatt ttttcttgag gaagttcaaa accagcctgc aaagctcctt
    13501 caactaattg tacctgtcct ctgatcaatg catttttttc tgtttctact actaatatgt
    13561 cttcatctcg aagcatttgt tctgctttgc gtattctgtc ctttgtcgga ataaagatca
    13621 caacagtctg tttttgcttg cgccaacggt ccatttctgt tttcagcaat ggcatttgtc
    13681 cgaagaactg ttgcatcgtc cgatattgaa aattatggat tgcttggaaa cgaagatttc
    13741 ccatcccttt ttggaagagt gagaaaaagg ttgtggtgaa cttttccttc tgcacgattt
    13801 tttgtatatc tgctgaaaat gtctgctcag aaaacacccg caattcgctg attttttgag
    13861 tctgccattc tgccgcttcc ctttcgattt cccgatttgt ttccatgatt cgctgataat
    13921 catcaacgac tagtaaacta tttgaaggga aataatccaa gatcgtcaca gattcttggt
    13981 aaagaagatc tgagtagaat tttgcttctt ctgtcgggac acctgcttgc cagcttgaag
    14041 tcagctgacc gaagtaatct tgcaaaaact cgcggtctgc ttcttctgtg gtcgcagcaa
    14101 gccgtttttc tagcattttt tgcatttgct ctattccatg ttctagatcc tctggtccgt
    14161 agacttgttc actcgttggg gaaacccaga tttcttccgt tttttcgatt gagcgttgtg
    14221 tttctgcttc aaaataccgc atagaatcta tctctacatc aaatagttct acacgaacag
    14281 gataggttgt cgttaacggg taaatatcga taatgcttcc tcgaatactg aattctcccg
    14341 gttttgcgac catctcttct cgtacatagc ccatcaaagt caattggcga ggaagatttt
    14401 ccacgtctat ctcatcacca atatgccaat tcaattgcgc ctgtagccaa gttttcttat
    14461 tcggcagtcg tttgtgcaaa gatgcgactg gcaacaaata gatacctgct tttttttctt
    14521 gaagtgcgtt aagcgttgca actctctcgc ctcgcgcttc tggagaagaa aaggccattt
    14581 cagcagaaag cacttcatcc actggaaata aatggatctg atcttgcgga atcacatgtt
    14641 gcagatcttc tgctaactga ttcgcataat acaaatttgg catcagaata atcatgtttt
    14701 tatttgtttt cttaaacgct cgagcaaata gaagtgtttt cgctgagccc gctaatcctg
    14761 tgattagttg tctaggcatt ttttcttcta aattgctgac ccattctttt acttgcggat
    14821 cttttccgac taattgaata atatccattc cttttcctcc ttcctcaatt gaatcgattc
    14881 atcgcatcca cgaacgaatg cccttctatg agatattcga ctgctttaac agattgacac
    14941 atgctttctt ctatcaaagg ctgattttct ttgctgaaag gactaagcac atgctggact
    15001 actgtttttt tcccttctgg cctgccgatt cccaccttga ttcgatcaaa gacattcgta
    15061 ttcaaatggg aaataatact tttgataccg ttatgcccgc cggcacttcc cttttgacgg
    15121 agccggattt ttcccactgc cagatccaaa tcatcgtaga ttacgactaa ttcctctgga
    15181 taaatcccaa aataagtcat tagtgggccg actgcacgac cagattcatt catgaatgtt
    15241 tgtggtttga ctaaaaggat cttttctcca ttatgaaaaa attcggcaac ttctgcttcg
    15301 aacggatttt ttttgaaagt agcttgatgc tcgcgtgcaa ttttatccac gaccataaag
    15361 ccgatattgt gttttgtata ttggtatttg gtacctgggt ttcctaaccc gacgatcatt
    15421 ttcatttttg ttttccacct aattctaaaa agcagcacaa aaggctggac tgctaattag
    15481 cggtccagtc catcgagctt ttttcttttt tacacttgtc aatgcatagt ataacacaaa
    15541 acaaggcgtt tcggctaaaa gagaatcgtt aataaatgat aaagaaatta aaaatttctt
    15601 caaagtttaa tgaaataatt gatatataaa aatagtgaaa taacgcacat gtaagctttt
    15661 acatataata caaccttgtt atctgttata ttttatagcc aaatagcgtg acatggggct
    15721 tgagaaatgt tagcataagc atgggaaaaa acaaatcaat ggaaggattg atactaaatg
    15781 actgcagctg cagaaaaaaa agatcatcaa aaagttattc ttgttggaga cggcgccgta
    15841 ggttctagct atgcctttgc tctagttact caaaatatcg ctcaagaagt aggtattgtg
    15901 gatatcaata ctgctaaaac tgaaggagac gcaattgact tgtctcacgc tttggcattt
    15961 acttcaccta aaaaaatcta ttctgctact tacgcagacg cacatgatgc tgacttagtt
    16021 gttattacag ctggcgcacc acaaaaacca ggtgaaactc gtttagactt agtaaacaaa
    16081 aacctaaaaa tcaaccgcga tgttgtcaca caaatcgttg aatcaggatt taacggtatc
    16141 tttttagttg ccgctaaccc agtagatatt ttgacttact caacttggaa attctcaggt
    16201 ttccctaaag aacgcgtaat cggttcaggt acctctcttg actcagctcg tttccgtcaa
    16261 gcacttgcgg aattagttga cgttgatgca cgtaacgtcc atgcttacat cttaggtgaa
    16321 cacggagatt cagaattccc agtttggtca catgcaaatg ttgccggctt gcaaatctat
    16381 gaatgggtaa aaaacaaccc agaaatcgat gaagaagcta tggttaacct attctttggc
    16441 gtacgtgacg ctgcttacac aattatcgag aaaaaaggcg ctactttcta tggtatcgca
    16501 gtagcattag cacgtatcac tcgtgccatc ttagatgatg aaaatgctgt tcttccttta
    16561 tcagtattca tgaatggcga atatggattg aacgacatct acattggtgc acctgctgtt
    16621 atcaaccgtc aaggtatcca aaaagtaatc gaaattcctt taagtgacag cgagcaagac
    16681 cgtatggctg cttctgctaa gcaattaaaa gaaattctag atgaagcttt tgctaaacta
    16741 gacgctgaat aatctttgaa catgacggaa aacatctatt cggtgttttc cgttttttta
    16801 ttttatcacc cttatggttt ctaaattttt tcataaagta gagataacct tcgtcttttc
    16861 ctctaaatta tgctatcatg agaccgttcc accctaaact tttacttaag gagttgtcga
    16921 tatgacaaga tcttcgcatc gaaaaaaatc gaaacatgtt gctttgcttt cagttcttgg
    16981 tgttctctta gtgctgatca gcttttatac ctatcgcagt acatactaca caaagcattt
    17041 tttgccgaat acagaaataa atggaataaa tgtcagcaat ctaacagtcg aaaaagcaaa
    17101 tgaaaaatta aaagaagctt attctgataa aaaactggct attaaagaag atggaaaagt
    17161 ctggcaggaa gtaccaaaat ccgagctcgg ctacaaagat gatttcactt cggagctttc
    17221 tccgatccta aatgaacaaa acggatggac ttggggaatg acttatgtct cagcggcaga
    17281 aaaacaagaa atagatcctg tcagtcaaaa ccaccaaaaa ctagacacta ccgttcagac
    17341 attgacaact aagctgactg atttgaacaa agaccggaca cctactactg atgcaacgat
    17401 cgaaaaagca ggtgactctt tcgttatcaa gcctgaagtc aatggaaata cgatcgacgt
    17461 cgatgctgcg gtcaaacaat taaaaagtgc ggtaaactct ggtaaagata caattgaatt
    17521 gactgaattc aaagaaaaac caaaagtaac atctgaagac agctcactga aagaacaact
    17581 cgcatccatg aatgctatcg caaatgtaaa agctacttac agtataaacg gtgagacttt
    17641 ccagatccca tcaagtgata ttatgagttg gctgacctac aatgatggaa aagtcgattt
    17701 agatacagaa caagtacgtc aatatgtaac agatttaggc acaaagtata acacaagtac
    17761 gaatgacaca aaatttaaaa gtacaaaaag aggagaagtt acggtcccag ttggcactta
    17821 cagttggacg atccaaacag acagtgagac agaagcattg aaaaaagcga ttctggcagg
    17881 ccaagatttt actcgttcac ccattgtgca aggtggcaca accgctgatc atccattgat
    17941 cgaagatacg tatatcgaag ttgatctgga gaaccagcat atgtggtatt acaaagacgg
    18001 aaaagtggcc cttgaaacag atatcgtttc cggaaagcca actacaccta cacctgctgg
    18061 agtcttctat gtttggaata aagaagaaga tgcaacttta aaaggaacaa atgacgatgg
    18121 aacaccttac gagagtccgg taaactactg gatgccgatt gactggacag gtgttggtat
    18181 ccacgattca gactggcaac ctgaatacgg tggagactta tggaaaaccc gcggttcaca
    18241 cggctgcatc aacaccccac caagtgtaat gaaggaatta ttcgggatgg tcgaaaaagg
    18301 aacgcctgta ttggtcttct aacaaaaata atatataaaa agaaagcacc cgtacgattg
    18361 gtacaggtgc tttcttttta ttatctaaca atgtgctact tacatttcag cagcagtaaa
    18421 gacttctgaa acatcatcat cgtcttctag cttatctact agacgttcca attgctcttt
    18481 ttgttcctca tttaaatcaa tcgtcgtttg tggaatcatc gtcaattctg cttgcgccaa
    18541 agtgaactct ttttccaact catctcgtac agcagcaaaa tcttctggtg cagtataaat
    18601 ctcgaatact tcaggagaag tttgcagatc ttctgccccc gcctctaata catcctcaaa
    18661 catcgtctct tcatcaacag tcaacccatc gcgttcaata gcgatatatc ctttgcggtc
    18721 aaacaagtaa ctgactgaac ccgtttctcc cagtgatcca ccgtttcgag taaaagctac
    18781 tcggacattt gtcgctgtcc gattacggtt atctgtcaat gcatggacta agattcctac
    18841 tccacccgga ccatatcctt catacgtgat ttcatcgtaa ttcgtttcat ctacggcact
    18901 cgtagccttt ttaatcgccc gttccacatt gtcgttaggc atatttgcag cttttgcttt
    18961 gtccacaaca agccgcaaag ctggattcat agatggatca ggaccgcctg ctttagctgc
    19021 catataaatt tcacgagaaa gtttctgaaa gactttccct cgttttgcat cttgggcatt
    19081 ttttcttcct tggatattat gccatttact atgtcctgac atgatttttc ctcccttgtt
    19141 cttttgagaa tacacttcta ttctagtact atttcacaaa aaaacaaagc cacagccaat
    19201 ctataaacgc ttcttgaatg ccgcaaaaag cacacataat atcacagcag tcagagcacc
    19261 gatcgaatcc aacatgacat cttggaacat aggtgttcgt ccaccggtca tcatctgatg
    19321 gaattcgtcc aaacctgcgt accctgttgc tgaaagccaa gcaaggatcg ctgtcagcca
    19381 ccaaattttt agcttgggga aaacacctaa aaacaagctg cctcccaaaa taaaataggt
    19441 accgaaatgt gcgcccttcc ttaaaaagaa ctctaaaaat ttggcgtagc ccagatggtt
    19501 gatactcacc atacttccac cataattaaa acgtataccc gctaatgtgt ctttaaatgg
    19561 ctgattaggc agccagcttt ctaatcgcga aacttgtgat tgctgtccgt aagtttgaga
    19621 agaactgtaa aataagatta taacgactat cagtgcgatt actaaataaa tatttccgtt
    19681 tttcatttgt ttttttgaag aattcataag tcacctctgt attttatctt agactttttt
    19741 tgaagaaaca acaatcattt tcatagagac ttcttccata aaaaagtgct atacttaatt
    19801 ttgtattaca atatgaaggg agttttttca tgagttattc attaaattgg aatctagatt
    19861 ctattttttc cggaggcagt cattctgatg ctttgaacca acggatgaag caattggaag
    19921 accaaatgaa cgagtattat caccgcgtaa caaaatggtc acctagttct gacaatgcag
    19981 agcagttaaa cgctattctt caattgcaag aaacgattac gaatggtttt acccaatgca
    20041 gcagctatat tactgcattg ctttctgcca atgtcaatga ttcagatgca aaggtcttat
    20101 caggcaagct ttacgcaatg ctgcctcgtc tgcaatcagc cgaaactgta ttgtcaaaga
    20161 aatttgcaga aatatctgac aatgattgga atcagatgct ttctcaatct tcgtttgaaa
    20221 caattgcttt tcgtttaaac gagattcgtc gtgatggctc acaattatta tcagaagctg
    20281 aagaaaacat tataaatacg ctttctttag atggtctaaa tgcttggagc agtcattatg
    20341 atacgattgt agccagcatt tcgattccct tcgaacaaga tggacaagta gtcgaattat
    20401 ctgctggaca agccttcaat aagatgatgg gcgatccaga tccaaaagtc agagaaacac
    20461 ttttcgcagc atgggaagaa gcctggaaag aaaaggcacc cctcttcacg gatactttga
    20521 atcatttaga tggttttaga ctttctgact atgaacttca tggtgtcaca gattttctac
    20581 aaaaaccatt agagtacaat cggttgaaga aagaaacatt gtctgtcatg tgggatacaa
    20641 tccagaaaaa caaacaaccc cttgttaatt acttgactag aaaagctaat cttttcggta
    20701 aagaaaaaat ggaatggcaa gatcaagatg cacctattat tttaggcgat ttgaaagaaa
    20761 aaacctttag ttttgacgaa gcagcagcat ttattatcga aaactttaac aaattcagtc
    20821 caaaaatggc taaatttgcc caatctgctt ttgaaaagag ctggatcgaa gcagaagatc
    20881 gtcctgggaa acgtcctggt ggttattgta ctgagttacc agaaacaaaa gaatcaagga
    20941 tctttatgac ctacagtaat tctgttaatg aggttgcgac acttgctcat gaactaggac
    21001 atgctttcca tagcagcacg atgtgggatt taccttcttt aaatcgcgaa tatgccatga
    21061 atgtcgcaga aacagcaagt acattcgcag aattgatcgt agccgatgct acattaaaag
    21121 tcgcaaaaac aaaagaagaa aaaatcaatt tgctagatac aaaactgcaa aatgctttag
    21181 ctatgttcat gaatatccat tcccgattca tttttgaaaa tcgattctat gaagcacggc
    21241 aagaaggatt agtctcagaa gataagatca cagagatgat ggttgaggcg caaaaagaag
    21301 gctatcaagg agctttagct acttaccatc catatttctg ggctgcaaaa ttgcatttct
    21361 ttattgatga cgtaccgttt tataacttcc catacacatt tggctacctc tttagtttgg
    21421 gcatctatgc ttacgccaac caaaagggag caaattttga agaagaatat atccatttac
    21481 tacgcgatac tgcctcaatg acgacggaag atcttgcgaa gaaacatttg aatgtcgatc
    21541 ttacccaacc agatttttgg caagcaggta tcgacatggt gactgaagat atccaaacgt
    21601 tcatggaatt gacagaagaa tttatctgat ttctatttta tcacaataaa aaaagagtta
    21661 gtccagaatg ggctaactct ttttg
//
LOCUS       DAYYHS010000048        21462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000048 DAYYHS010000000
VERSION     DAYYHS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.048"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..1093
                     /locus_tag="ACHA6A_001742"
     CDS             101..1093
                     /locus_tag="ACHA6A_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8969933.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1153..2574
                     /locus_tag="ACHA6A_001743"
     CDS             1153..2574
                     /locus_tag="ACHA6A_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8969934.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2567..4066
                     /locus_tag="ACHA6A_001744"
     CDS             2567..4066
                     /locus_tag="ACHA6A_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8969935.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKLIVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4105..5499
                     /gene="uxaC"
                     /locus_tag="ACHA6A_001745"
     CDS             4105..5499
                     /gene="uxaC"
                     /locus_tag="ACHA6A_001745"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8969936.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            5658..6341
                     /locus_tag="ACHA6A_001746"
     CDS             5658..6341
                     /locus_tag="ACHA6A_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8969937.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            6343..7179
                     /locus_tag="ACHA6A_001747"
     CDS             6343..7179
                     /locus_tag="ACHA6A_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8969938.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            7190..8944
                     /locus_tag="ACHA6A_001748"
     CDS             7190..8944
                     /locus_tag="ACHA6A_001748"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8969939.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(9220..9561)
                     /locus_tag="ACHA6A_001749"
     CDS             complement(9220..9561)
                     /locus_tag="ACHA6A_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8969940.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(9629..11800)
                     /locus_tag="ACHA6A_001750"
     CDS             complement(9629..11800)
                     /locus_tag="ACHA6A_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8969941.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            12011..12868
                     /locus_tag="ACHA6A_001751"
     CDS             12011..12868
                     /locus_tag="ACHA6A_001751"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8969942.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(12939..13796)
                     /locus_tag="ACHA6A_001752"
     CDS             complement(12939..13796)
                     /locus_tag="ACHA6A_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8969943.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(13781..16483)
                     /locus_tag="ACHA6A_001753"
     CDS             complement(13781..16483)
                     /locus_tag="ACHA6A_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8969944.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(16496..17785)
                     /locus_tag="ACHA6A_001754"
     CDS             complement(16496..17785)
                     /locus_tag="ACHA6A_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8969945.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17935..18981
                     /locus_tag="ACHA6A_001755"
     CDS             17935..18981
                     /locus_tag="ACHA6A_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8969946.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            18983..21136
                     /locus_tag="ACHA6A_001756"
     CDS             18983..21136
                     /locus_tag="ACHA6A_001756"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8969947.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         6340 a         4346 c         4085 g         6691 t
ORIGIN      
        1 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
       61 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatat
      121 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
      181 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
      241 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
      301 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
      361 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
      421 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
      481 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
      541 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
      601 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
      661 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
      721 caatccagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
      781 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
      841 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
      901 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
      961 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
     1021 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
     1081 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
     1141 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
     1201 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
     1261 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
     1321 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
     1381 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
     1441 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
     1501 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
     1561 cccccacaga gcttcccggg caaactgaca gcctttttat accgacgttt caaattaaac
     1621 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
     1681 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
     1741 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
     1801 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
     1861 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
     1921 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
     1981 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
     2041 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
     2101 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
     2161 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
     2221 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
     2281 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
     2341 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
     2401 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
     2461 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
     2521 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
     2581 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
     2641 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
     2701 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
     2761 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
     2821 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
     2881 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
     2941 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
     3001 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
     3061 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
     3121 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
     3181 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
     3241 attaatagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
     3301 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
     3361 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
     3421 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
     3481 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
     3541 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
     3601 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
     3661 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
     3721 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
     3781 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
     3841 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
     3901 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
     3961 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
     4021 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
     4081 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
     4141 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
     4201 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
     4261 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
     4321 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
     4381 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
     4441 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
     4501 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
     4561 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
     4621 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
     4681 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
     4741 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
     4801 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
     4861 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatcgt
     4921 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
     4981 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
     5041 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaaccgt
     5101 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
     5161 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
     5221 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
     5281 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
     5341 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
     5401 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
     5461 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
     5521 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
     5581 cggaccaaat ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt
     5641 atgaaagaag agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa
     5701 aagatgaaaa caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac
     5761 ccgagatcca gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg
     5821 aaggaaggtt agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat
     5881 ttggacataa cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt
     5941 taaaaaatct gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac
     6001 tgacatctgt gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg
     6061 gagactatcc aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg
     6121 tgttctcaag atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct
     6181 acctgcatct tatacctgat acttgtaaaa attacccaga aggactaaaa gacaataccc
     6241 actttagtcc agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat
     6301 cccgtctgcc tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta
     6361 ggcatccgtg cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca
     6421 cagcttcact tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa
     6481 ttaactgaca tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat
     6541 atcgaacttt ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt
     6601 gcacaagcat tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag
     6661 atgattgcta cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa
     6721 gaagcttatc agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt
     6781 ggcgtaactg ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct
     6841 aaacgtttaa tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat
     6901 ttgatgcata aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat
     6961 ttacatggtt atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc
     7021 cgcatggtac ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa
     7081 tatgagcgtc ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa
     7141 gcaatagaac acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg
     7201 aatacagaaa agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta
     7261 tcagggaaat tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc
     7321 atcttggtta atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc
     7381 ttctcagttg caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa
     7441 tatattgacg aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc
     7501 tggactaaga gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga
     7561 attgcaatct ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt
     7621 gtcctctcaa gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg
     7681 tatcacgctt tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc
     7741 cgcattcgtt actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca
     7801 agaaatggaa gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg
     7861 agctcatggg ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta
     7921 tgaaaaagga ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag
     7981 ttttcagata tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg
     8041 catccaaccc ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt
     8101 ttctggaatg atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat
     8161 tggcgaactg ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca
     8221 tttggatgga aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc
     8281 gattatcgat catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc
     8341 atctggctct ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc
     8401 aggaaaaatt tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa
     8461 ttatttagcc gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat
     8521 cacttatggc ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca
     8581 actttcttca ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg
     8641 caacaagctg tctggtggcc aaaagcaacg tatctccatt gcacgagcgt tgatacgtca
     8701 gccaaaaatc attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa
     8761 aatccagcaa gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg
     8821 gctttcaacg atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga
     8881 aataggtact tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga
     8941 atgacaaaaa ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg
     9001 gtgttctcct gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt
     9061 tttttgaaat tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa
     9121 tggtgttcaa gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga
     9181 acgtctcttt cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg
     9241 aactggtgtc atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt
     9301 ttgcatcaag tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt
     9361 cattgcatct tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg
     9421 catcgcttgg aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta
     9481 cgcttctttc aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc
     9541 gctatcgtag atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt
     9601 aacatggggt tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt
     9661 tagccgcttc accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc
     9721 cttcttttgc ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat
     9781 tagaatttcc tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg
     9841 catctgctac actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt
     9901 tgaagacttt cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt
     9961 cttcgtaacc catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt
    10021 catttgcttt tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat
    10081 aacctgcagg attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg
    10141 tcatttcatc ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga
    10201 cgatcaccgc tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac
    10261 catcattggc aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca
    10321 gtccaccaag ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt
    10381 ctgctttatc atatcctttt tgtaaaccga tctcatgcaa aagccataca gtaggcaaat
    10441 tcaaactttg tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat
    10501 tatgcgcatc gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag
    10561 cttccagtgc tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca
    10621 tctgtgtagc aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt
    10681 gtacaccacc tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg
    10741 aagcatttgc tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat
    10801 cctgatctaa agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac
    10861 catactcgtt aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag
    10921 aatctgaatt atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt
    10981 gttcttgttc ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc
    11041 gatcattcgc attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc
    11101 ctgccaaagt agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc
    11161 tggaagcgtc ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg
    11221 tcaagatttc ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg
    11281 cttttcgatc aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga
    11341 tcgtacttcc tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg
    11401 caatcccttt gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta
    11461 ctgcatcttg gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt
    11521 acagtgtgcc agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg
    11581 attttaacgt ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta
    11641 agacacagat caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc
    11701 gctttcgcca tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca
    11761 aagcagcttt tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat
    11821 ttgattcaat cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta
    11881 gttcaatatg tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg
    11941 taagtttttt cttaaatatg ttttaaaaat ggtacaatta gtctgcgatt gcactgaaag
    12001 gagtacactt gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg
    12061 cgacttgctt gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg
    12121 taaaaacgtt tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga
    12181 ccagatcaca ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga
    12241 tgcgaagcac atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc
    12301 aggaatgaag actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc
    12361 tgcttatctc gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac
    12421 aagcggtgcc gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt
    12481 ggaacaaaaa atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga
    12541 tatgaccatc accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga
    12601 tgataaaaat ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca
    12661 aacacaagtc tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc
    12721 tcataaggga catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact
    12781 gatgctccat gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg
    12841 taaagcttta cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat
    12901 aatgataaga ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct
    12961 gctaacagca aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat
    13021 tcacttactt gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt
    13081 ttttgaagga gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct
    13141 aacgtcaacg tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca
    13201 ggatgatctt gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct
    13261 gctgctaatc cttctaaaca atctttatga taaggacaag taccttcata atggtcatct
    13321 ggatggcgtt tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct
    13381 tcattgatca cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt
    13441 tttccttgtg ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc
    13501 caagcagcag gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac
    13561 ccttttttgg gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc
    13621 gggccgaatg agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc
    13681 tgatccaatg tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca
    13741 ccatctgaaa ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt
    13801 tttcctcctc gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt
    13861 gcttcgttgg ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt
    13921 ccaatttttc taaatgacta gacatattga aacctcttac tactacttta tcactaaact
    13981 tgctgcgttt caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt
    14041 ttgaaatcaa tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg
    14101 cgtatgcatg caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat
    14161 tgaaccgatg ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta
    14221 gttctcccac actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt
    14281 cattcaaacc aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg
    14341 cttgctgatg ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct
    14401 tcaccacttc aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca
    14461 agacacgtaa gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt
    14521 tactatcttt tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc
    14581 gttctgcttt tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt
    14641 ctgcagaaac agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat
    14701 ctactaatac ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga
    14761 aaggttgttt aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat
    14821 tattcaactt tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg
    14881 tcaaatgtag agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt
    14941 cttctgcttt cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt
    15001 tttctaatgg taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt
    15061 tcggcaaatc ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc
    15121 cactttttgt ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt
    15181 gttcaggtat tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac
    15241 cagttttatc cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc
    15301 cggtcgtatc gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa
    15361 cttcatctgc tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac
    15421 cgcaaatact gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat
    15481 catggggata gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt
    15541 ctagctgtcg ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat
    15601 tggctaaagt ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta
    15661 agtcttctgg cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa
    15721 actcgtaatc tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta
    15781 ccggttgatg atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg
    15841 catccgctaa tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat
    15901 tcccgttgct ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt
    15961 tccaccacat ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa
    16021 agccaattgg ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta
    16081 attgaggcgt ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg
    16141 ttccccattt acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc
    16201 tgataagaaa atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga
    16261 ttgcttgttg gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa
    16321 tcgtttgccc atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta
    16381 gatcgtctat caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac
    16441 gatcccagtg gctatgagaa atgatatata ctttcttttt catttacgat acctcctact
    16501 tttctactct aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag
    16561 aaaaccattc tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt
    16621 gtgttcctgc gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg
    16681 acgttgtcat tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg
    16741 aggatccaat tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca
    16801 atgttcttct tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg
    16861 cggcgatcag attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta
    16921 cttcatatgc ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc
    16981 cctcttggat ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta
    17041 tcgtttgaaa aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta
    17101 ccagatatcc ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg
    17161 taaaagctgt ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt
    17221 ctcgtacaaa tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga
    17281 tttttttgat accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt
    17341 aaagaagata ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc
    17401 agatccaatc gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt
    17461 tgaaggcatt cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc
    17521 tgatcatgtc agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg
    17581 cagtagagtc tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt
    17641 cattttgctt tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg
    17701 cccagtccgc atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg
    17761 attttggtat atctttataa accactaata aaaccttctt tctatttgat atatctttaa
    17821 agattatact tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact
    17881 ttttcatcca ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact
    17941 caaccattgt accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta
    18001 cctgtagaca gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga
    18061 atcacttcca aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc
    18121 gggaaaggca gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa
    18181 acacgacgga ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc
    18241 aagggattga accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac
    18301 tttctacaaa ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat
    18361 gctcttgata cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct
    18421 gtcgtcatat tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat
    18481 ttccaaggcg gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct
    18541 tatttgtttg gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt
    18601 gaagcattgg acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca
    18661 ttagatgaat tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac
    18721 gatgttgtcg ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa
    18781 gatttttcaa ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg
    18841 acaaccatcg cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact
    18901 tggttagaga caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga
    18961 caatcaacaa aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc
    19021 gaatcagcca aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat
    19081 gaattatttt gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga
    19141 ccgcgttttt caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag
    19201 tcatatgctg tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca
    19261 aagttcttac cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt
    19321 acgttatggc atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga
    19381 ttacagcaaa aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt
    19441 tatagatcct tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga
    19501 tttttctttt tattttatat tgagatttga atatccttta acaattgatg agttagctgt
    19561 ttccaacaaa aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat
    19621 ccgatttggg acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca
    19681 aaaccaaagc aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga
    19741 tcgtatacag ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata
    19801 tcgggtgttt ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca
    19861 ctatgatacg actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg
    19921 ggatacgtat aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga
    19981 aatgctggaa ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc
    20041 tcctgatgaa cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc
    20101 tgtaaaagat atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc
    20161 tacggttcaa agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata
    20221 tggctatgtt cctaatcact accacgaatc agtcaatcac acattagact atgcttatag
    20281 cgatttttgt attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata
    20341 tcagcagcaa tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc
    20401 aaaagataaa gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta
    20461 tgcagaagga agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat
    20521 cggaaaatat ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc
    20581 cccaacgttt gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc
    20641 gatagaattt ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt
    20701 cagttatata ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac
    20761 cttcgacgat tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg
    20821 gtatatcttc aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat
    20881 cggtatgcca ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat
    20941 tgaagcaacg cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag
    21001 agaatatacg aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta
    21061 ccaattaggg atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt
    21121 atcaaactat aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    21181 gccaagcaaa aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa
    21241 gcttttgaac accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag
    21301 agacaaatgg caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca
    21361 gctgattgaa caccatttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa
    21421 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa at
//
LOCUS       DAYYHS010000049        21148 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000049 DAYYHS010000000
VERSION     DAYYHS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.049"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1667
                     /locus_tag="ACHA6A_001757"
     CDS             30..1667
                     /locus_tag="ACHA6A_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969948.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1812..2552)
                     /gene="truA"
                     /locus_tag="ACHA6A_001758"
     CDS             complement(1812..2552)
                     /gene="truA"
                     /locus_tag="ACHA6A_001758"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8969949.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2743..3378)
                     /locus_tag="ACHA6A_001759"
     CDS             complement(2743..3378)
                     /locus_tag="ACHA6A_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8969950.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3525..4745)
                     /gene="sstT"
                     /locus_tag="ACHA6A_001760"
     CDS             complement(3525..4745)
                     /gene="sstT"
                     /locus_tag="ACHA6A_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8969951.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4964..5962
                     /locus_tag="ACHA6A_001761"
     CDS             4964..5962
                     /locus_tag="ACHA6A_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8969952.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6158..7993
                     /locus_tag="ACHA6A_001762"
     CDS             6158..7993
                     /locus_tag="ACHA6A_001762"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8969953.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8053..8769)
                     /locus_tag="ACHA6A_001763"
     CDS             complement(8053..8769)
                     /locus_tag="ACHA6A_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8969954.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8996..9538)
                     /locus_tag="ACHA6A_001764"
     CDS             complement(8996..9538)
                     /locus_tag="ACHA6A_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8969955.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9543..9770)
                     /locus_tag="ACHA6A_001765"
     CDS             complement(9543..9770)
                     /locus_tag="ACHA6A_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8969956.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9849..11729)
                     /locus_tag="ACHA6A_001766"
     CDS             complement(9849..11729)
                     /locus_tag="ACHA6A_001766"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8969957.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11918..12532)
                     /locus_tag="ACHA6A_001767"
     CDS             complement(11918..12532)
                     /locus_tag="ACHA6A_001767"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8969958.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12674..13234
                     /locus_tag="ACHA6A_001768"
     CDS             12674..13234
                     /locus_tag="ACHA6A_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8969959.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13539..13853)
                     /locus_tag="ACHA6A_001769"
                     /pseudo
     CDS             complement(13539..13853)
                     /locus_tag="ACHA6A_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14107..15742)
                     /locus_tag="ACHA6A_001770"
                     /pseudo
     CDS             complement(14107..15742)
                     /locus_tag="ACHA6A_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16181..17050)
                     /gene="scrK"
                     /locus_tag="ACHA6A_001771"
     CDS             complement(16181..17050)
                     /gene="scrK"
                     /locus_tag="ACHA6A_001771"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8969960.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(17070..18191)
                     /locus_tag="ACHA6A_001772"
     CDS             complement(17070..18191)
                     /locus_tag="ACHA6A_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8969961.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(18193..18588)
                     /locus_tag="ACHA6A_001773"
     CDS             complement(18193..18588)
                     /locus_tag="ACHA6A_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8969962.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(18607..19404)
                     /locus_tag="ACHA6A_001774"
     CDS             complement(18607..19404)
                     /locus_tag="ACHA6A_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8969963.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(19406..20194)
                     /locus_tag="ACHA6A_001775"
     CDS             complement(19406..20194)
                     /locus_tag="ACHA6A_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8969964.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(20208..20684)
                     /locus_tag="ACHA6A_001776"
     CDS             complement(20208..20684)
                     /locus_tag="ACHA6A_001776"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8969965.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(20756..>21148)
                     /locus_tag="ACHA6A_001777"
     CDS             complement(20756..>21148)
                     /locus_tag="ACHA6A_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8969966.1"
                     /translation="RHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAI
                     YILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQ
                     SFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         6678 a         4182 c         3262 g         7026 t
ORIGIN      
        1 tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata
       61 tcatttttat gttatttcta agttttgctt acgtcatccc tctattcatg tctaagggaa
      121 tctatcatag tgtcaatcag gatacttact ttcatttatc tcgaatcata ggactagata
      181 atgtatggag ttctccagta aacttcaata acttcgatca tcatggaaca atgatgaata
      241 ttttttatcc atggcttact ctttatccag cttttttatt ttataaaatg atgggaaatc
      301 ttgttttaag ttataatatc tactattttt ttatcacttt tttaacaatg gtcgtttctt
      361 atttttctat gaaacaaata aaaaacaatc gttatatttc gctgttattt tcgattattt
      421 atacattttc cgcctatcgt gccattgaca tattccatcg tgcttcattg ggagaagctg
      481 tcgctttaac atttttgcct ttgatactaa tggggtgtta cgaaatttat attagagatt
      541 accaaaaatg gtactggttg tctattggca tgacgctagt agtgtatact catttacttt
      601 ctgtcgctat ggtttctgta tttattggag gaacgttgtt tctttctttt tatttttggg
      661 accaaaaaat cgctagactc ttaagtcttt taaaagctac cgtcctcacc ttttttttaa
      721 gtgcgggatt cttaattcct tttattcaac aatctagagc acaagaattg aaagtcccac
      781 tgggaaaaga attaagtggt atggctccta gtgatatgct gacacatatt ttaaataata
      841 actataacaa ctatactatt ggattattct tatttctcgg attgattggt gcttttattt
      901 ttataaaaaa attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt
      961 tttgttcaac caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac
     1021 aatttgtctg gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag
     1081 tgatctatta tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc
     1141 ttgcactaat attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat
     1201 taacacttat agcacctgaa gacgctgttg ctatagctga aaattacaat catactgatt
     1261 atgcaaacaa agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag
     1321 gtaaccagga aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag
     1381 ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag
     1441 tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat
     1501 taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa
     1561 ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat cgatttactt
     1621 tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag
     1681 aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat
     1741 ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta
     1801 tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt
     1861 tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg
     1921 gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat
     1981 ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca
     2041 atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt
     2101 gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa
     2161 taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg
     2221 tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt
     2281 ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca
     2341 atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt
     2401 cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt
     2461 tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg
     2521 tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt
     2581 ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga
     2641 cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc
     2701 ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa
     2761 gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc
     2821 tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc
     2881 cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc
     2941 attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa
     3001 acctgatccg atattatgga cccatgtcga gacattccac gaagattcat gggtattgat
     3061 actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac
     3121 tagggtagct aacacacgag atgtactaac aaatgcagca tacaatgcag gcaatgacag
     3181 gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga
     3241 aacctctcca aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc
     3301 taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa
     3361 tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat
     3421 tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat ctatataact
     3481 cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat
     3541 ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct
     3601 cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg
     3661 ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt agagatacca
     3721 aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca
     3781 caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg tgcttcaatc
     3841 cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata
     3901 ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt
     3961 tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga
     4021 agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa
     4081 aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt
     4141 aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc
     4201 aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct
     4261 atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc
     4321 aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga ttgaacagga
     4381 ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt
     4441 tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct
     4501 acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc
     4561 ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt
     4621 gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg
     4681 gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac
     4741 gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt
     4801 atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa
     4861 ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag
     4921 ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt
     4981 ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat
     5041 ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc
     5101 tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt
     5161 tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt
     5221 aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg
     5281 caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct
     5341 aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag
     5401 tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact
     5461 attattttta acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc
     5521 attacagaat ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt
     5581 gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt
     5641 gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa
     5701 tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt
     5761 ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa
     5821 ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta
     5881 tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa
     5941 tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta tctttttatt
     6001 ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt ttgattcgat
     6061 tgaacaatta actcagcgtt ggaaaaaaag aattcattgg ctttttgttc ataaaagtta
     6121 ttttttagtt gcagcatttt tttactgctt taccttcgtg cagattttcg ttttagaacc
     6181 aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa atatttactc agcgacaagc
     6241 acccatgatc ctgacaatca tcattattat gggatttttc ttcttactat tgttgatttc
     6301 caatcgtttt tggtacgcct tgactgctac cttgatcatt aacttactgc tcactatttc
     6361 gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct
     6421 gaccggattg tcagaactat tatcgatggt tagcccagtc ttattaatcg tgggcggatt
     6481 gatccttctc ctattattga ttactagtat tattgtacaa cgtagactac aaaaacagta
     6541 ttccttgaag attcattgga aacgtcgatt tatcagtatc gctgtattat taggatgttt
     6601 ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt
     6661 taaagtaaac aagactttct ttaaccagca aggcgccgta aaagataacg gaccagtcat
     6721 tcaattccta aacaatatcg acattaaaat catggaagaa cctgctggct attcgaaaga
     6781 aaaaattaat ggaataatga aaaagtacaa cacaaaggca gatgaaatca accaaacaag
     6841 aagtgactgg ttaaacaatc agacgatcgt tctcaattta agtgaaagct tcagtgatcc
     6901 tgcacgtgtc cctaatctga cagtacctac agaccctatt ccttacatca aaagcattat
     6961 aacagaagga acaggaggat tgatgttatc cgtcggttat ggtggcggaa cagctaacat
     7021 ggaatgggaa ggactgacag gcttgagtat cagtaacctg tctgcttcgt tagttacacc
     7081 ttatacccag ctagtggagc gtcagaaaat aagtccaaat attaccgatc tattcgatga
     7141 aaaaatagcc atccatcctt ttacagctgc tctttatcgc agaaaacaag tgtttgataa
     7201 atttggtttt gacaggttct attatttaga cagtccggat aaattaactt atacggataa
     7261 aatagaacaa agcccacgca tatcggatga ttcagcatac aaagaaacct tgaaagcttt
     7321 acaaagcaat acggaaacgt cacaattcat ccaattatcc actatgcaaa accatatgcc
     7381 ttatgatgat tactatagtg aattgaacta cactgctgaa ggaactgctg tcattcctaa
     7441 tagaagaaaa gaattacaaa cttatatgca aggccttcac tataccgata cagcagtaaa
     7501 aaaattcatc caagaaatcg acaaaatcga taaaccgatt acctttgttt tttatggtga
     7561 ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa tatggattag aacagcacca
     7621 gacagattat ttcatttata acaacaagta tagtcgtgaa caagcagcta cgttaaataa
     7681 agaagttgtt gctcctaata actttggtgc tatggctctt gagcatgcga atatcaaagt
     7741 aactccttac tatgcgttgt tgacagaagt atctaatcat ctacctgcat taacaattga
     7801 tcctacagaa agcttgtcta atcgttttaa tggaaaacag gtctttgtta cagaggatag
     7861 tcaaattatc caagaaaaaa atttaactga gaaacaaaaa gaaattctag aagattatcg
     7921 tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa
     7981 aataaaagac taacaaataa actgagcaag gggttgtgat aaaagacatg tcacaacccc
     8041 ttgtttatct cattaatttg cttgtccata aacgatttga ttaaagccat ttttcaattc
     8101 attattcgct gtctcgttga agtaaagata attatcggga ttcccctgat tttgttgtgt
     8161 caccaatact tttggctgat tttgctgaaa cccgaataga tagacgtgct ttttcagata
     8221 tggttctgtc tctgcatcgg aatagacggc tgtgatttga tagtctgctt gacctgtccc
     8281 actttctgac cattctgcag aaacaggcac tccaccaata gccatcttcc actccccgtt
     8341 caaaatagct tgagggactt taagaccata taagtccaca ctcatttgat tcgtataact
     8401 tttgtattgt tgtcccattg tttggcccca tttggtcata aatgcctcta attgtgcgga
     8461 tttgtctgca ttccacagtg tttgttcaac ttgttcgctg ctttctgaag aggaagatga
     8521 agcctctaca gaagaacttg aagtagatgt ttgagttgtt tcagtcgttt tggaagactc
     8581 tgacgaagta gaggtatctg ctgtttctgt acttgctgca gttgacttgc tctctgtgct
     8641 agttgttgta gtagttgcaa ctgttgcttt cgctgtttct tttgttgatt cttttttcga
     8701 ttcattcccg ttggtacaac cagctaacaa aaaagctaga ccaattgtta ctaaaatcct
     8761 ttttttcaat taaaaaactc ctttcgatta ctcccacttc tttattctat caaataagca
     8821 atgtgatttt attgattttt cgtattttct catcaatctt tcaaatcttc taaaaactga
     8881 gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg cttcttttat tttaatcttc
     8941 gtgattttcc aatttacctc acgctttata tttgtatttg aagaaacata gacttttagt
     9001 catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt tccttggagt ctttgtactt
     9061 ggtagatgat ttctttattg aaacttaaaa gttcaacaag caattgttgc aagccatatt
     9121 tttcttcttt ttcagctaat ttgattgccc gtgtgataaa gacattttgg aaatcaaaat
     9181 cttttgcaaa gttttccaac attgttgagg ctggtaaata tttgttttga ccatgttcgt
     9241 aaatttttcc ataacgcatg acttcttcta aagtagacgg aacgatttca ttttcaatca
     9301 ctaactcatg agagatttta ttgaaccatc tcgcttcttg atcagcatat tctgaaaaaa
     9361 gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa ttgtgcttgg atcaatttca
     9421 tttcttctaa tttaaaattt gctaatacat gccccaccat ggcgccagca gttggtttat
     9481 gatggtcgat atctgcttgt tttgtttctt gaacaaattt ttcttccact atattcattg
     9541 atttacgcct cttttacttt tgctgattgg acttcataac ctaatttttc cacaactgca
     9601 gctaattgtt cagggtggac ttcatctgga tcaaagtttg ctttgatttt gctcgcatta
     9661 aacaaaactt tcgtattgct taccccaggc tgttgagaaa ccgcatgttc gattttttgc
     9721 atacagcttg gacaagctag tgtatctaat tgcataataa tttttttcat cgtagttctt
     9781 ccttcctttt tcttgatagt cttattctag attacagctt ccataaaaaa attgattctt
     9841 atcaagtttt acgatttttc ttctttgctt tcgtgtccaa aatacaataa acgcatcgcg
     9901 ttaaagataa cgatcaagat actagcttcg tgaacgaaca tcccactagc catgtaaata
     9961 aacccggcaa ataagccgtt gagtaaaaat aagacgacac caatggcaat cactacattt
    10021 tcacgggtat ttaaaacagt tttcttagcc aagcggtagg catgaaccaa cgattcaaaa
    10081 ttagattgca tcaagacaac gtcactcgtt tcgatagcaa catccgttcc tgatcccata
    10141 gcaatcccga tatcagccgc agccaaagag ggagaatcat tgattccatc tccaataaaa
    10201 gcaacgcgca atccttgttc ttggtatttt ttcacaaatt gaaccttttc gtctggtaat
    10261 aattcagcat ggacttcatc aatgcctaac atatcagcta cataatcagc agtcatctga
    10321 ttatcacctg tcagcatcac taactgtttt gctcctgctt gtttcaactt agctaattgt
    10381 tcagcgactt ctggtcgaat cacatctgac acacctatga tttgagtgat ttttttgtcg
    10441 attgctacaa taatgaccga gctgcctgtt tgttgtaatt gatgtaaatc ttgtacttgc
    10501 tttggtgtga gagaaagttt gtgagcggca atcaatttgg tgttgcccgc acaaactgta
    10561 tgaccatcaa tctcagctgt gattccttgt cccttgattg tctgattatc tttgactgct
    10621 aattgttgaa aatcaatgtc tttctcctga gcataatgga tcacggcgcg ccccagagga
    10681 tgatcggata acgtttctaa tcgagccact aaagctaagc tgtccccttc atcctctgta
    10741 tagttcttga tagtggatac agcagtagtt cctttggtca atgtaccagt tttatcaaag
    10801 acaaaagtat cgattttgga aaaagtatcc attgcttcac cgcctttgac taagatgccc
    10861 cgtttcgctc cattaccgat tcctgctaca ttcgaaacag gcgcaccaat gactaaagcg
    10921 ccaggacaac caagaaccaa aacagtgatt gctaaacgaa aatcacgact aatcagacca
    10981 acaaccaacg caataagtaa aacggttgga gtataatact gagaaaatcg atcaatgaat
    11041 ttttccgcat gggatttggt atcttgtgct tcttctacta attcgataat tttggcaaag
    11101 gtcgtatcat ctccgacctt agttgcttca atcgtcacgt agccatctgc taaaatcgtt
    11161 ccagagaaga cttcatctcc actgattttg acgacttgtc gtgattcacc agtcactgcg
    11221 gcttcatccg catacccttt cccgtcaatg actcgtccgt ccacaggaat agctccacca
    11281 gtttttacta agacgatatc gccttcttcc acgtcatcga tttccacttc ttcagtcgtt
    11341 ccatcttctt ggacaacact agcagtagat ggggccatct cggtcaaagc cttgattgac
    11401 tcccgcgttt tggtcaatgt tttttgttcc agatagctac caaacaaaaa caagaatgta
    11461 acgatagctg actccttgta ttctccgatc ataaaggcac caagcacagc aatagtaacg
    11521 agtaaatcaa ttgagataac tttgactttc actgcctgat acgcatggat cataataggt
    11581 atgaccgcaa taatagaagc tatggctaga gcaatgttat agaacaagac aaaaccagta
    11641 aattttccga taaaaccaaa aataatcaac acagctgata aggcggtgat atgatttcga
    11701 tgctcttgga tccacttttg taatttcatc tttctccctc ttttcctttc attcttacga
    11761 gactaagtat agatacttat ccatcataaa aaattgacca ctatcaagtt tagtcaaaaa
    11821 ggcggttttc ttttggtaga atacactaca aaaaatagtt agcaagaaga ctcactaaaa
    11881 gtgatcttgc caactattgt cgtttctatc gaaattttca ttgaatgatt ttcctgtaca
    11941 gcatacatcg gagatctatt tctttatcta agatttttat cttgctaatt ttttccttta
    12001 gtctttcaaa tccacgtcgt tcatagaatt gatacgtaca gccactatct gtaaaaagga
    12061 atatttcttt tcctttctct cttttttcaa attctgttag caacttggta ccgatgcctt
    12121 ttgttttcaa atcaggattt gcagctaaaa aaatgatctc tccatctggc gaatgcttct
    12181 ttaaatatgc tgcaagcatt tgttcatttg ctgtctcgta agctccttta ctttctttag
    12241 caaatatatt ttgtaaaaaa tcgaatatct taacatatat ttgctgtcca aaagaacgat
    12301 gtttcttttt ttctcctttt atctccgcca gcaaaacacc tgccaactcg tcctctgcat
    12361 acaaagcaag aatttgagtt gctcgattca actccatata caaaaaatat cttccgtaaa
    12421 gattcagcag aagttcattt tccatatatc tctcaaagtt cattccttca acagcaaaat
    12481 agatagcttt tttgtaatct ttcttttcta agggtttaaa agttagtttc atcatttcgc
    12541 ctcctcaact tcaatgattg tgttatactt acattataat agacacagtg tctactatct
    12601 ataatcaatc atctataagt ttgtcgatta agcgacagct ttgctttgat atgtcggata
    12661 aggagatagg taaatgaaaa agacagatag acgcgtcaaa aaaacagaaa aagctttggc
    12721 tgaaacacta tccacattat tagtaaataa aaagattcaa gcaatcacta tacgcgaact
    12781 aacagaaacg gcagatgtac atcgttcgac tttttataca cattataagg atatctatga
    12841 tctatatgat caattagaaa gcaacttttt tcgggatcta aatgaaattc tctcttatga
    12901 tccagcccat agctatgagg aactatacac tcgcttgatt gactatgttt acgcaaatac
    12961 ttttttatat cgcttattta tcctcaacca tggtgatatt tcgtttcaga agaaagttaa
    13021 acaagttatt gaagaaaact atctgaacat atggctatat gaagacaaga aaacgattat
    13081 cacagaagaa atgagattct ttactacgta ccacgtccaa ggttgtctat ccgttctcaa
    13141 tcggtgggta gaaagcaatt acaagtactc caaaaaagaa ttattagaac tcttacagaa
    13201 actgaatgat cacatagaga aaatcatgcc ttgaataaaa acatactaag cttagttaag
    13261 aagaaccgta accttctcgt ataacaactg aatcttattt agccaaattg tttactgaag
    13321 attttttgag taaaatttct ataattccta cttattgaaa agtagtttgt agtgctgctt
    13381 ctccctctct aagtgatata tcttgttgta ccaatcaaat tcacctcatc attaataaat
    13441 tacttttagg ctaatttatt acaacttata ggttgaagct gtaaccaatt tatagtcata
    13501 ttatattttt atcatccagt ttattgaatt cttctcgttt acctcaattc attttcaagg
    13561 ggaatcagct catcatctaa cattttacgc catgctcgct tcatttcttg tgggtattta
    13621 aatggtttcg cttctggatg tttggcttta tatccttcgt aatccttact gttgataatt
    13681 gctttcatat taggaattgg atcttcacct ggcatatatt gcagattaga agcatgtaat
    13741 tttttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc acttacttgt
    13801 gtaaaatagc gacgtttaat gacaaagata tcttcattgc tatctagtgt accttcattt
    13861 ctttgtgctt aaattcaaag ataattttct caataaagat ttttgattcc agtaagattc
    13921 ctttactcct tttacaacat agcgttgtcc tttgttttga aacatcttct tcccctcttt
    13981 gccattaaaa atagcccaat tctctataaa gaagattaag cttcattatt ataatataaa
    14041 gtttaaattt ggtttattac ttactttttc ttattctcta gtaagctaat attgaaaaaa
    14101 atgtaatcag atcattattt ttatatgttg taacttcctg ataagaaaac taattaaaag
    14161 aaagaggtaa ctacataaac taattaaaaa agctaacttc atagttggag gataaatgga
    14221 agaaacttca atgattgttt ctccttgtgt tccttcaatc gacactagtc ctattatcaa
    14281 tctctctttt ttgtccattt acttttgcga ctgttcccca ataatcaaat acaggaagcg
    14341 ttattttttg tcctttagat tcatgtactg tatagactat tttcgaatcc atcacttgat
    14401 aaggcatagt atgccattcc ttgccaattc ttacttcatg attctgcaaa tgttctttgt
    14461 attgtaatgc ttccgtgggt gcatagtcat aaagtcctcc gggaaggagt gtcgtgtaac
    14521 gaataagatt ttcttgattc attaccaagt gatctctctg agtgacaact ttatgttcat
    14581 agtttgcttt tgttgcagca gatacagcca acagtaataa aacaattgcc gttatattaa
    14641 ctacttttct ttgcttagct gatgtgctct tcatgaaaac gaacgataaa atcaacgagc
    14701 caaacagtac ttgaaagcct aagattcgcc aaggaaattg aatcatttga atgggtgtat
    14761 tttgtaataa agaccatgga aaaaatttgg tggataagag ccaagagact accgcactaa
    14821 ggtaaatcac tttttctttg cgattaaatc ctttaaaaaa gaagagtgga cagatcaaag
    14881 aaataatacc aatgattcct accgtaaagg ttgtcaggtc attattcatc gcaccaatca
    14941 aactatccga gacatttaat gcttggtgtt gtaaggtcgt tttatctggc tcattaatcg
    15001 attgaaccat gatttgtcgg aacatcggat accaaaagta tgctgttaaa agaatcgtca
    15061 ttcctactgc ctttgacaac tccacaatca accggatgct ttcttttttt cttgtttcac
    15121 ttactacaaa gccgcctaag actagtaagc caacggctat tacactcata aaagtggaaa
    15181 gaacatgtgt ataaatgagt aagctcatac tgactgctaa tggaattgca catggctttt
    15241 tctcataaat cagtgcgtaa gcataataca taactatcgg tataaccgta agtgcaatcg
    15301 cttcagcaat agcagaacga tgataaatat caatcgtacg gtacccataa aatgtatata
    15361 aacagctaaa tataaaagcc gcatcgattc tttttaaaaa ttttagtcca taatgaaaca
    15421 tcagcatgat agtacaaaca ttcaatagcc aaacatataa gatataacta acgattaaat
    15481 tattacttat ccaataaaaa atgactgccg gaaaaaaggt caagtaggga taaaaataat
    15541 tcactccgct cccataatga ttaaacgtcg taaaattgat tggaccagaa aaaattgaag
    15601 ataacccttt gactctagca aaatggaagg caatatcccc cgatgtagaa atagtatgaa
    15661 catttctaaa ataattgatt gaataaagtc cagcaataat tgctaggaga atggacaata
    15721 ttattttttt ctttacttgc attacttaat tatatactcc tttcatggat tgctttatct
    15781 agtataactt aaattttaaa taaatcgtta tttattttta ctctcactta aaaaagcact
    15841 aaaattataa gagagggagg taatggtctg aacaccaaga attgttccaa catcgaattg
    15901 cttaactaat gagttcaaag cttaattatg tggctcttat atttaccgag cgtggattgc
    15961 gatctcttta gcgaaacaaa catctttcat aaaaaaaaca gccatatccg ctttcttgga
    16021 tatgactatt tactactact ttactatctt tcattcttct tgtctcactt atgataaggc
    16081 gctgggtatc atgagtgaat gatattgctg aatacaaata gtccgaccac attaagttga
    16141 tcggacttca taagcatttt attgactaat acttttttgc ttaagaattg attctaacat
    16201 cttttgctaa agttaagcat cctatagttc ctgcattatc tgctaactta ggtgtgacaa
    16261 tgtattcttc gatatttggc gtatcgacat agccgttaat tagttctaaa aaacgtcttt
    16321 gtactttctt tttcagatgt tcttgttgca tgacaccacc accaaaaatt ataatatctg
    16381 gtgaaagcat cagggtcgta ttacaggcac attgtgcaat ataatccgcc tcgatttccc
    16441 agaacggatc ttctatagaa agatcttgtc ccttggttcc cgtacgcatc tcgatagcgg
    16501 gtccagatgc caacccttcg agacaatcat gatgaaaagg acaagtacca gagaaagtat
    16561 ctcctgaatg tctcttaact aacgtatgcc ccatttctgg atggctaaac ccttcaacaa
    16621 aaatcccatc ttgtatagct cctccaccaa ttccagttcc aacagtgtaa taaacaacac
    16681 tggataaacc ttttccatga cctgaaacat actccccgta acaagcagca ttcacatcgg
    16741 ttgtccaggc aattggaatt tcgaaatgct tattcaacgt tccaacaaaa tcaaaatttt
    16801 tccaggcagt ttttggagta gaggtgatat gtccataggt ctttgattca cgatggatat
    16861 caatcggtcc aaatgaccct actccaatgc tttctaattg ttctttatag tgattaaaga
    16921 aatcaattac caaactcatt gtttcattcg gtgtagttgt aggaaaactc actctttcta
    16981 aaacctttaa atcatcattg ccaatggcac aaacaaattt agtcccacct gcttctattg
    17041 agccatacat ttcttactcc accgtccttt tatctaggaa ttcgttcttc cctgtctaat
    17101 agtttttcga aaaactcatg tggttccttt tggtaccaat aggcaacaga agaaatatcg
    17161 tcttgacgct cataaagctt gatgtcatca tttcctaggg tttggaaagt aacacgtaaa
    17221 ttttctttga aactaatggg gtcgggtaaa tgccagcggt acaacccatg catcggtaaa
    17281 gcatcatcgc caaaaccatg aactggattt ggattaggtt ttccagcact aaaataatcc
    17341 cgggtgtgat ctcttttaga ttgaaaagga tagcccataa atagcgtatt aaaattttga
    17401 gcatatgccc tttctccaaa gttcttttta tggaaagccc aagcgccgcc aaaataatct
    17461 tctgctcctg tagatgttat tgtagggaaa tcttcatctc catctatata aaacttaaat
    17521 tctccttccc cccaccagta tctttccaat gcgcacaaag caaagtaggt tcctacataa
    17581 tacccagtgc ctttgattcc atcaataagg gtgtaatctt ttccttttgt cgtgatgggt
    17641 tcacggttcc atttagcatg aaaatacatc gcatcatccg gtacttcagt caatgcataa
    17701 ttaaatgtat aaaaaatgtg cttaataaac tcaggatgtt cattcgttaa agtaatctta
    17761 gctttttttc gaaatggcat ctcaaaatag caattcatcc ctgatgttgg attcactacg
    17821 attggtaagg aattcacttc gcatcttgtc ccaaacccat tacaaaaaaa atctcctaat
    17881 ggactttcta cagaaggagt ggattcatca tcccaataaa tacgcaaaat taaatcacgt
    17941 aagacgaaac tcccagcgat tgttttgtct gtcactgtca tccaaaaatg acgtaaaatt
    18001 ccagggcctt ccatttctgc taaagtaatt gtctctccag catttatcgg aacagaaggt
    18061 gacccttttc tcccaattcc tagatgactc gctgtcattg ccgctttccc tttttcacca
    18121 tttttatttt ctggtgaaat agttctactt tcctctccct tgtggagcca tatattattt
    18181 aaacaattca ttttattcgt cctctatcct ttcttcactt ttttgattat tcatatagat
    18241 gatttgttgt tgtgcctccg caataattgc tccaatatct aaattatcat ttcctaactg
    18301 caaagcgatt gacattgcca agggaagatt cattcctgca attacgtgca ctctgtcaga
    18361 aatataggta aaaaactttt gatttacact tcctccagcc atgtcagtta aaacaattat
    18421 ttcgtctgta gtatccacta actctttaat ttgttcatct atatcatcaa tttcttcgac
    18481 atatgcacat aagctactaa tttgattatg catacctgtt aaaaactcta gtgtctcttt
    18541 aaaaccctca gctagtattg aatggctagc aatgatcaat cttctcataa ttcaaatcct
    18601 tttcctttac gctaatattc caaatgcagc cataaccatc gcaaaaacaa taacgagtag
    18661 aatcaaaatg gtcatactga tttttttctt actgagtaga taatatgctg cagcagtaat
    18721 aattactgga attaatgttg gtaaaatttt gtccaacatt ggttggattt tcatactaac
    18781 ttcaccaaaa gtgaatttaa gtggcgttga aacactgata actgaagcaa ttaatgatcc
    18841 aactactgtt agccccaata ctgaagcagc ttcagttaaa taattaatct tttcaccgta
    18901 ttcagtaaca atcttcattc cctgcttata accaaattca aagcctctca ttcggaaaaa
    18961 gaagattacc atgattaaaa tgaaccataa aaagattcct acaggattat tttctaaagc
    19021 catataagcc gcaattgatc ccatgatggt tgggatcatc gtcatcaaaa gagagtcccc
    19081 aataccagca attggaccca tcgtaccaac tttaaagtca ttaatcgcat cttttgcttc
    19141 taatccttgc ttttcctcca ttgcaacaca agcacctaaa ataatggctg ccagccaagg
    19201 ctgtgtattg taatactgaa agtgattatt taaagcttcg atgtattcat catcatcttt
    19261 gtataatttt cttaaaatag gtgacaatgc gtaagtcaca gatgcacctt gctgtgtttg
    19321 ataattaaag gtacaaactg acatcaacca acgatagttc gctttcctaa gatccttttt
    19381 cgttacttga tatttttcgc tcattttatt catcctcccc aataaaccca acatcagaag
    19441 ctcccgcaga agcagtgatt tgtgcatctt tttcagctct atgatacaca ttaaatgcta
    19501 aagcgaatcc tatgatcgcc gctccaataa taggtacctt caaaaaagcg gctaagaaaa
    19561 atccaacaag taaataacta atgtatcgac ctgtaggcat aaagcgtaac aacatcgcaa
    19621 taccaacagc aggcaacatt tttccggcta atgttagccc atccataaac caattaggta
    19681 aaacatccaa aacgtaatta acagcagact gtcctaaagt aacggcaata aatactggaa
    19741 ttgctgcaga taaaccaaag aaaattggcc ccaaccacaa aacactattc attttcttaa
    19801 attctcgttt attcgcatat tcctgtgatt tgcgaacaac aaaaccattt aagattttga
    19861 caattacatc tagttgcacc cctaacatac ctacaggtag tcctattgct aatccggctt
    19921 ctactccaag ctggctacca aatgccgttg caataattgc gcacgttcca tagtccggta
    19981 cagatgagcc accaaaaccg gcaactccta atgacatcaa ttgaactgta ccgccaacta
    20041 tcaaacctgt ttttatatca cccatcaaaa ttcctgtgaa taatccccaa aaaactgcgt
    20101 aatttacaaa gatcatcgga atcccataat aatcaacatg tttaataaat gctaatatag
    20161 tcagcataat aatttgaaaa atagtaaaat gcattatcct tactccttta tcctaatttt
    20221 gattgaattt cctgtgcctt atccgccgga acaaattgtg taacaatttc tacaccagaa
    20281 tccaacaatt ttttatacgt gctgatattt tcatctgatg cataggctct agaagatagc
    20341 tgtaatgcat tatctttaac aacagtaccg ccaacaatta gtcggtcaat tctaataccg
    20401 ttatctacta aatgaaggat tgtttccggc tctttcacga tgacaaatac tttttcctga
    20461 tcgtatttcc cctttttaaa attcgcaatc gctgtttctt cagtgataat tgatacagcc
    20521 attccagcag gtctagctaa acgcatactt gatttttgga tgtcgttatt tgcgacagaa
    20581 tcatcgatca ccatcactct gtttgcttta gacattggtg cccattgcgt gacaataatc
    20641 ccatgcaata aacggttatc aactctcgac ataacaatac tcataaatac ttcctccttt
    20701 atctttcatt ttcttacaag caattaccat gccaaaacaa aaagaacttg ataaatcaag
    20761 ctctttttac ataatcataa acgtatttaa cctcatgaat cgggatgtcg acactataat
    20821 aatgttcaac gacactgaaa gattgcttaa aatgtgtctg aaatttttga aattcctcac
    20881 tcgtttcatt gaaataaatt tcatcttcca aagtattttg ttttactaat cgttcaatta
    20941 agcaactaat gtgaacatat aatccgaaac acgtattatt atcaaatgaa attcctagat
    21001 cattttgtaa gatgtatata gcatctgaaa cccgttctaa taatttagtc ggatttaata
    21061 tggtcaattg ctccattaaa ttcgttagac taaaattctt cagaatatta ttgttcaaaa
    21121 ctttcagttc attatcatta aagtgtct
//
LOCUS       DAYYHS010000050        20634 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000050 DAYYHS010000000
VERSION     DAYYHS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.050"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHA6A_001778"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHA6A_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8969967.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHA6A_001779"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHA6A_001779"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8969968.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHA6A_001780"
     CDS             complement(2298..2960)
                     /locus_tag="ACHA6A_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8969969.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHA6A_001781"
     CDS             3144..4790
                     /locus_tag="ACHA6A_001781"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8969970.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHA6A_001782"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHA6A_001782"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8969971.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7524)
                     /locus_tag="ACHA6A_001783"
                     /pseudo
     CDS             complement(5971..7524)
                     /locus_tag="ACHA6A_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7672..8813
                     /locus_tag="ACHA6A_001784"
                     /pseudo
     CDS             7672..8813
                     /locus_tag="ACHA6A_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8939..10018)
                     /locus_tag="ACHA6A_001785"
     CDS             complement(8939..10018)
                     /locus_tag="ACHA6A_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8969972.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10121..10561)
                     /locus_tag="ACHA6A_001786"
     CDS             complement(10121..10561)
                     /locus_tag="ACHA6A_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8969973.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10618..11028)
                     /locus_tag="ACHA6A_001787"
     CDS             complement(10618..11028)
                     /locus_tag="ACHA6A_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8969974.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11208..11642)
                     /locus_tag="ACHA6A_001788"
     CDS             complement(11208..11642)
                     /locus_tag="ACHA6A_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8969975.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11742..12590)
                     /locus_tag="ACHA6A_001789"
     CDS             complement(11742..12590)
                     /locus_tag="ACHA6A_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8969976.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12979..14745
                     /locus_tag="ACHA6A_001790"
     CDS             12979..14745
                     /locus_tag="ACHA6A_001790"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8969977.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14847..16106
                     /locus_tag="ACHA6A_001791"
     CDS             14847..16106
                     /locus_tag="ACHA6A_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8969978.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16253..17554
                     /locus_tag="ACHA6A_001792"
     CDS             16253..17554
                     /locus_tag="ACHA6A_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8969979.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17557..18408
                     /locus_tag="ACHA6A_001793"
     CDS             17557..18408
                     /locus_tag="ACHA6A_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8969980.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18421..19236
                     /locus_tag="ACHA6A_001794"
     CDS             18421..19236
                     /locus_tag="ACHA6A_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8969981.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19387..20553
                     /locus_tag="ACHA6A_001795"
     CDS             19387..20553
                     /locus_tag="ACHA6A_001795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8969982.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         6841 a         3731 c         3831 g         6231 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt
     7501 tttaatcgct tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt
     7561 gaaaatatta ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa
     7621 tctctactat cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat
     7681 tcaaccatct taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca
     7741 gtccacacag aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat
     7801 cgaattacct ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa
     7861 ggacaaacag cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta
     7921 caatcagctg ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac
     7981 actttgacta tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac
     8041 ttgttaaaac cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat
     8101 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga
     8161 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
     8221 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
     8281 gaaactcaac cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca
     8341 gatgtaactc cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca
     8401 gacacagata atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca
     8461 gatgtgactc cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac
     8521 aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta
     8581 aaaataaatg aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct
     8641 gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc
     8701 taccaaaatc cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa
     8761 gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga
     8821 ataatgtctg tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat
     8881 accagattga aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt
     8941 aagggatatc tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta
     9001 aaaagaaggt aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc
     9061 taacaagtaa gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact
     9121 tcggattcag cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa
     9181 caacatctcg gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga
     9241 taacacctgc tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct
     9301 gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa
     9361 caaattgagc ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa
     9421 tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt
     9481 ttttcttttc aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca
     9541 tttttcctat atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa
     9601 aaaaatcgtt tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg
     9661 gctgatgctc gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca
     9721 tcgggatata ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga
     9781 tagctaaaac acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca
     9841 tcaaataagt aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa
     9901 gaacaaaaac gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac
     9961 gctgataaac ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta
    10021 tatgatggaa tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    10081 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    10141 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    10201 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    10261 caccttgagc agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    10321 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    10381 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    10441 caagatactg ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga
    10501 tttctttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    10561 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    10621 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    10681 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    10741 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    10801 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    10861 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    10921 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc
    10981 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa agttaccacc
    11041 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    11101 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    11161 acatatacaa aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc
    11221 tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat
    11281 aaataaccaa aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc
    11341 atttcttgaa tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca
    11401 gagacgatga tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca
    11461 agagacgtgt tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca
    11521 ttattgcctc tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga
    11581 tacagttcta gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc
    11641 ataaagatct cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta
    11701 aagaaaacgc attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa
    11761 gtaaatccgc gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg
    11821 atttcttcta tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa
    11881 acctcttttg actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca
    11941 atctcagcac ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca
    12001 gatttctttc gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata
    12061 gataaaatag cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg
    12121 aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg
    12181 aggggaggga tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct
    12241 aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt
    12301 tctggaacta ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag
    12361 ggttttttac ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata
    12421 actatagctc ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct
    12481 ccagcagcaa tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg
    12541 gcaaaaataa tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt
    12601 ccttaattca ttaataatac tcttatctta ttataactta cttttcacct taagttaact
    12661 ttaattaata aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac
    12721 ttctacgcca aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt
    12781 aagataaatt caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa
    12841 gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata
    12901 acatttttga aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa
    12961 aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat
    13021 gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc
    13081 aaaaaagtat atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa
    13141 aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa
    13201 gtgacgagcg aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg
    13261 gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca
    13321 aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg
    13381 gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca
    13441 aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga agactttttc
    13501 gggggagatt tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat
    13561 ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat
    13621 tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa
    13681 gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat
    13741 tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc
    13801 cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa
    13861 aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat
    13921 ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt
    13981 gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga
    14041 gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct
    14101 caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg
    14161 attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat
    14221 catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct
    14281 catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg
    14341 atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg
    14401 atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa
    14461 gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag
    14521 attatttctg aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat
    14581 ttcacacgga aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct
    14641 tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac
    14701 gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta
    14761 acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca
    14821 cgttgaatac aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag
    14881 ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag
    14941 cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag
    15001 attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag
    15061 tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg
    15121 gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc
    15181 tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga
    15241 cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata
    15301 acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag
    15361 acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga
    15421 ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg
    15481 atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt
    15541 atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag
    15601 gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat
    15661 ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc
    15721 taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt
    15781 atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc
    15841 aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag
    15901 caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag
    15961 cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg
    16021 atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca
    16081 gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg
    16141 gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg
    16201 ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca
    16261 agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat
    16321 ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct
    16381 attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg
    16441 aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat
    16501 cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc
    16561 gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat
    16621 aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac
    16681 gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca
    16741 tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt
    16801 tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt
    16861 atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa
    16921 atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc
    16981 ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac
    17041 tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa
    17101 aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc
    17161 ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc
    17221 agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt
    17281 cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc
    17341 catgatctat ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac
    17401 tgatattctt atttcctgga tctataaact tacgactgga acttctccgc agtattctat
    17461 ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt
    17521 taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct
    17581 cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc
    17641 attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca
    17701 gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt
    17761 gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc
    17821 gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc
    17881 cgcaaatcaa gtctgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg
    17941 actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca
    18001 atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac
    18061 actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt
    18121 tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct
    18181 tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat
    18241 cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct
    18301 ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta
    18361 caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc
    18421 atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg
    18481 ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg
    18541 atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa
    18601 tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa
    18661 ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg
    18721 gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag
    18781 aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat
    18841 tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta
    18901 gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt
    18961 ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg
    19021 atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg
    19081 ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac
    19141 tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt
    19201 gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca
    19261 atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac
    19321 ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg
    19381 aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct
    19441 gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc
    19501 caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcatct
    19561 ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca
    19621 ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa
    19681 tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat
    19741 tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt
    19801 gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat
    19861 atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg
    19921 ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta
    19981 agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa
    20041 tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca
    20101 agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta
    20161 aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc
    20221 ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt
    20281 actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta
    20341 cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca agtccaagat
    20401 caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct
    20461 tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct
    20521 ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact
    20581 atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaa
//
LOCUS       DAYYHS010000051        20345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000051 DAYYHS010000000
VERSION     DAYYHS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.051"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1216
                     /locus_tag="ACHA6A_001796"
     CDS             14..1216
                     /locus_tag="ACHA6A_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8969983.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(1393..2661)
                     /locus_tag="ACHA6A_001797"
     CDS             complement(1393..2661)
                     /locus_tag="ACHA6A_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8969984.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     regulatory      complement(2712..2779)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(2822..3469)
                     /locus_tag="ACHA6A_001798"
     CDS             complement(2822..3469)
                     /locus_tag="ACHA6A_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8969985.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(3734..4237)
                     /locus_tag="ACHA6A_001799"
     CDS             complement(3734..4237)
                     /locus_tag="ACHA6A_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8969986.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4361..5125)
                     /locus_tag="ACHA6A_001800"
     CDS             complement(4361..5125)
                     /locus_tag="ACHA6A_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8969987.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5233..5508
                     /locus_tag="ACHA6A_001801"
     CDS             5233..5508
                     /locus_tag="ACHA6A_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8969988.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(5856..7007)
                     /locus_tag="ACHA6A_001802"
     CDS             complement(5856..7007)
                     /locus_tag="ACHA6A_001802"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8969989.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(7027..7749)
                     /locus_tag="ACHA6A_001803"
     CDS             complement(7027..7749)
                     /locus_tag="ACHA6A_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8969990.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(7742..9193)
                     /locus_tag="ACHA6A_001804"
     CDS             complement(7742..9193)
                     /locus_tag="ACHA6A_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8969991.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            9335..10417
                     /locus_tag="ACHA6A_001805"
     CDS             9335..10417
                     /locus_tag="ACHA6A_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8969992.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            10751..11677
                     /gene="yidC"
                     /locus_tag="ACHA6A_001806"
     CDS             10751..11677
                     /gene="yidC"
                     /locus_tag="ACHA6A_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8969993.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMKMQALKPQVEATQQ
                     KLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARYTEGI
                     RESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAMIVFM
                     SISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPRKDVT
                     PAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(11858..12262)
                     /locus_tag="ACHA6A_001807"
     CDS             complement(11858..12262)
                     /locus_tag="ACHA6A_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8969994.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12278..13087)
                     /locus_tag="ACHA6A_001808"
     CDS             complement(12278..13087)
                     /locus_tag="ACHA6A_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8969995.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(13074..13964)
                     /locus_tag="ACHA6A_001809"
     CDS             complement(13074..13964)
                     /locus_tag="ACHA6A_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8969996.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(13977..14456)
                     /locus_tag="ACHA6A_001810"
     CDS             complement(13977..14456)
                     /locus_tag="ACHA6A_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8969997.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(14473..16239)
                     /locus_tag="ACHA6A_001811"
     CDS             complement(14473..16239)
                     /locus_tag="ACHA6A_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM8969998.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(16285..17226)
                     /locus_tag="ACHA6A_001812"
     CDS             complement(16285..17226)
                     /locus_tag="ACHA6A_001812"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8969999.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(17223..18218)
                     /gene="lacD"
                     /locus_tag="ACHA6A_001813"
     CDS             complement(17223..18218)
                     /gene="lacD"
                     /locus_tag="ACHA6A_001813"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8970000.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(18211..19404)
                     /locus_tag="ACHA6A_001814"
     CDS             complement(18211..19404)
                     /locus_tag="ACHA6A_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8970001.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(19422..20150)
                     /locus_tag="ACHA6A_001815"
     CDS             complement(19422..20150)
                     /locus_tag="ACHA6A_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8970002.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         5881 a         4409 c         3575 g         6480 t
ORIGIN      
        1 aggagttcat tcaatgaatc ttactagaga cctgatattt ttagttatct atattttagg
       61 aatgtcaggc atcctctatt ggcgcaagcg ccaaagcgat cccgctctat tcttttattg
      121 tgggacgtgg acttttatga tgatggtcac ttacttcgtc gttttagaag ttttttatca
      181 tacattttat tttttgattc cattgctatt tttcagtatt tttgcatttt tctattttaa
      241 ggaaaaacgt aggttaatca atggcgtact ttttaatacg tttttgattg tctttggtgg
      301 ttatctaggg tggctactaa tagagacgca aaatatcttt atcggtgggt tattggcttt
      361 gatcgggata ccaatattaa ttcttttggc aatcggtatc tacattttga tcattttttt
      421 attttggaat ggtgttgtag tcatgaaaaa agaatcccgt tctttggcca accttttgac
      481 tctcttgtta ggagtcgctt tgactatctt tttgatattg gactatacat tagtccattg
      541 gctcccacaa tgggcaaatg ctctatttgc tgtcattccg tttatttttg cttatctact
      601 catcgttttc tttaatttta tgactgtttc aattttgtat cagttcaacc gtccaaggta
      661 taaccaagat tatatcgttg ttctcggcgc tggcttgatt gatggtgaac gcgtatctcc
      721 cctacttgct aaaaggatca ataaggcgat cgcattttat tgggcacaaa gtaaagcaac
      781 tttgaatccg ccagtcttgc tcatgtcagg aggaaaagga accgatgaaa aagtagctga
      841 agccattgca atgaaacaat atgctttaga aaaagggatt cccgagcgtg atattttagt
      901 agaaacgaat tccacaacga cgctagaaaa tatgctgttt tcaaaagaaa taatggatca
      961 gcaaatgaat gggccttatc gcgcgatttt tacttcaaat aattaccata tctttagagc
     1021 tggactatat gcgcgacaag cgaaactcaa tgcaaacgga atcggtgcaa aaactgcact
     1081 atactactta ccgaatgcta tcttaagaga atttattgcc atccttgtct tacacaaaaa
     1141 acgccatctt atcatctgtg gcttaatgat tggctttgca gtattttctg ctcttattct
     1201 caaatttgct caataaaaaa gagcgtggga caaaactaaa aatcagtttt gccacagact
     1261 ctaactccga ataaacggtg ggagcagaag caactccttc ggaaataagc gaaattcaca
     1321 aaaatttaaa aagcaatttt cgtgaatttc ttcttatttc tcggagttaa acacttctgt
     1381 cccaacctct ttttatttct ctttttgtcg tgagattctt ccaaggatcg gtaggaacaa
     1441 gattcctttt ttcacttcaa ctttttgcgc agaatagatt ccactatttc ctgaacacat
     1501 aaagctaatc aaacagatca taaagaagta gctggctgct tgacctccaa aaagttcgat
     1561 ccccataaca aagcaagcaa tcggtgtatt cgttgctcca gagaacaccc caataaaccc
     1621 taatcctgct aaaaaaggta ctgatacatg aagaatgggt gctaatgtcg cacctaaagt
     1681 tgccccaatc tcaaacaaag gggtcacttc gcctccttgg tatcccgcgc caagtgatag
     1741 aacggtaaag ccaagtttcc caagaaaatc ataaggatga gcacttccat taaatgcatc
     1801 ttgtaataaa ggtaagctta atccgagata tctttgcgtc tgtaaaatca ctactacgag
     1861 taccacgact gctcctccaa taaagttgcg taaaactgga tgggggatcc gctttgcata
     1921 ccaagctttt aaccagacga tcgaacggct aaagagccag ccaatgatac caaatgcaat
     1981 gcccgcaagc aacaatttga agaaaagtga agggtcccat gcaggaatcg cccccatttg
     2041 ataatgggta tgcttgacat tgaaactttc cgttacaaag ttcgcaaaaa gtgctgcaaa
     2101 aaagcttgga aacagtgctt cggttctcac ttttccgatt gccaatactt ctaatccaaa
     2161 caatgtccca gccaagggtg tcccaaatac tgagctaaaa cctgcgctga tcccactgat
     2221 gacaagaatc tcacgctcga ttttgttcaa ttttagccaa cgagcaaaat tctcagcaag
     2281 tgccccgccc atttggaccg ctgtaccctc ccgaccaaca gaaccaccaa aaagatgcgt
     2341 ggtaatggtg ccaaataagg tcagtgggat cagccgtaat ggaatctttt catttcctcc
     2401 attcccttga tcgatcacta aattgtttcc ccgggaagaa ttgcccccat acgtcgtata
     2461 tagataagca aaaagtcccc cacttatcgg caaaagaaaa agtaaccaag gatgctccag
     2521 ccgaatagct gtcacatcag tcaaactagt tagaaagaat gcggataaac ttcccataaa
     2581 aatacctaaa attgctgtga tcaataacca tttgatcata tagatgatca ccttcaatgg
     2641 atcaggtaat cgattttcca tctcatttcc tcctcaaaaa aacgcctact aaaaaaaggg
     2701 gtagccccgt ttctttagta ggcgtcatta gttatcttac gatcaacgtt taaaggcgaa
     2761 caccatcacc tattcacttc accaaataag atagcatatt tttcagaaaa atacaatgaa
     2821 atcaccgctc acgtttcact cgttctgtga attcgtaaaa aggagctaat tgtaccaact
     2881 gctgattaca ttggtcacta tcatgacaaa tataatttcc ttttttcgtg tatgttccat
     2941 ctgaaccagt ttttgtggta gaaagaaaga gtgaaacatt tgaagttctt tgacaaatcg
     3001 aacaaattcc tttgttgatt gtaggagata atgttccact gatccctttt agtttttctt
     3061 cttcataata gatcataaat tttttttggc tacctggatc attccaacca atataagtat
     3121 gttcccttaa atccagttga ttccaatctg gttgttttaa ctttttcact tttcggaata
     3181 atttatcgat ctgcggtttt gtcggttgta tgaatggaag aacgatcact ttcaattcct
     3241 ctaatagtcg ttctgctttt tcttttttta gagtgacgtc tagtaccttt tccataaatt
     3301 catttacagc tgtattagga tgaccaagta cctcgtggat cttttccgca gctaatgctt
     3361 ggactgtttg gactgtcttt ggatcattca ctgaactata ggcgcttaac agctgctcga
     3421 cttctcgctt gataaaataa tattgatatg gtttgattgt tttttccatg actttgagtc
     3481 ctttcttctt tcaaaatcgg ctactttgaa gaaaaaagtg atctttctat caaagtgcct
     3541 ttagataaag ccctcaaact cttttaaata agatacacat cgcatcttat catcaccccc
     3601 tttattttta tgttggttat gtactttttc atctcaacaa agtaaacact aaaaagacca
     3661 attagtcaat gtctaaacaa aaaacccgag gatttttttc aaaatactcg ggtcaaatac
     3721 ttagttaact accttaaatc gattgttctt taccgaaata tcgttgccat ttttgtttca
     3781 ttgaagctgt cattggttga gctgcttctt tgactgcaca atatttatcc acaaaagtaa
     3841 cgatataacc ttctttatat ttaggagggg caatcgttgc tccccacata tgtttcaaaa
     3901 taatatcacg ctctagatct gaaagctccg tgattttttc agcatttttc acagcgatcc
     3961 ttgggtgaac atacgcatgt gtaccttcat cgaacttcgt cgtacgccaa tcgtagtaaa
     4021 ataaatcatg taaaaggcct gctcgagcag tcgctcttgc atcaccgccc cattttttcg
     4081 ctaatttgta gctatagtaa gacacactga ttgaatgctc taatcgtgtc gagtggacat
     4141 gttgcgtgta atttgctaat ttctttactg catcagtttc taatagatca tttatataag
     4201 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     4261 gaattccatt tatctttgaa attgaactta gtataccact agtgttcgta atattctagt
     4321 gaggttttcg caaaatgcag aaagcttaat agttccttaa ctatacggag aagtgataaa
     4381 gcatgatgcc cgctgcaact cctacattta aagactccgc atttccttga atcggtatat
     4441 acacattttt atccgttagc gccagcactt ctggacgtac gccttgacct tcattcccca
     4501 tcacgatcgc ataattttct gttgtctgga tatcattcag ggacactgct tcttcattca
     4561 gttctgttcc ataggttata aatccttgct ctttaaggat aggcaatagt tttgttagat
     4621 cctcttggat gatcggtaaa tgatagttac tcccctgcat gcttcttaat actttggtac
     4681 tgtagacatc cgccgttccc ttacctagta caacacaaga tagaccaaaa gcatcggctg
     4741 tgcggatcat tgtccctaca ttgccgggat cttgtacgtt gtcgagcaac aaccaaccac
     4801 cttcaaaatt cggtatctct cttttttcag cttccaccac tgccatgatc ccttgcggag
     4861 ttggtagatc tgaaacagtt gccatcactt cttctgatac tagtgtaaca gaaagcgcct
     4921 gattttcttc aagccaacta gcccactcag tcagaccgcg ttgcgtaata aaaagttcgt
     4981 tgaccttcgc atttgaggca acagcctcct cgatcaggtg aaacccttca agcagatatt
     5041 cccctttttc ttcacgatat tttttcttat gtaatttttt taattctttg attcttggat
     5101 tttttgctga ttggatctct ttcacatttt tcacctcaac tgattatacc atggattaga
     5161 tcattgcact tgttcagtag caaaaaagtc ggtatcatta atttaaaaat aaaagaaaga
     5221 acggtgaata aaatgaacaa aatcaagatg aacgtgcaag gacgtgtaca aagcgttggt
     5281 tttcgctata tgacaaaaat cgtagcggat caattaggga tcaccggaac agtgaaaaat
     5341 gaagatgacg gctctgtcac gatcgaagct gcgggcgacg atgaggcaat caaaaaattc
     5401 gtggaagaag tcaaacgatc accaagcccc agcggacgtg tccaatatgt agaccttcag
     5461 gaaaatccga tgatggagga cagaaaaaag tttgttgtaa taggctgacc ttctagaaat
     5521 gaataggaaa tgtcagaaaa atcggactgt ctgtatgtag atcgaaaaag tttgcccctc
     5581 tctccatcct agatcgacaa aaaaacattc ttattagcga gtttagtaac cagtgatagg
     5641 catacgtaaa agcgggagga cttcaaaaat tttggttcaa taaaatggag tctataatat
     5701 tttatggtag tggaaaatcg actttgattt tccactacca ctttttcatt gtcaaatcct
     5761 gacgctgccc agtcaacaag ccaactctcg aaaggatcat tttccctctt tgttctaatg
     5821 gatacccata gctgttttca aatatacagt tttatctaaa aatttagttt tgctaaacga
     5881 tcaaaggctt cacctaagat ttccaatggc tgagtcgctg ctagtcgaat atagttgtcc
     5941 ccgatcgttt ccccaaacgc ttttccaggg acaaaaagta cgccgctttt ttctaaaacc
     6001 tcttccacaa acgttactga atcgagccca ctcttactga tattaataaa agcatacatg
     6061 cttccagaaa cagaagataa agataagtaa gggatttctg ccactctttt ctcaatataa
     6121 gctaatcgtt ctttgaatac ttcaacaact ggcggtacaa gctgttcata gtgctttaag
     6181 gcgtagattg cacctctttg agaagcagtc ggtgcggaat aagtgatgtt ttcattgatc
     6241 aacccagcaa tttgattgat gtatgctggt gcgatcatgt accctaagcg ccagccagtc
     6301 atcgcaaacc ccttcgatac accactgaca gtgatcgtat tatcgggcgc aaatcgcgcc
     6361 atcggtacga acttctcttt aaaggcaaag tcttcataaa tctcatcgga aatgatatag
     6421 agttcatttt cgattgcgac ttcagcgatt tttttcaacg tgctttcaga aaaaacagct
     6481 cccgtaggat tattcggcga attgatgacg atcacctttg tacgatccgt aatcgctgct
     6541 tttaaatctt ccacatccaa ctggaagccc ttttcttcat aggtaggaac aaaaacaggc
     6601 ttgcctccag ataaaatgac ttgttcccga tatggtgaaa aataaggttc atggatgatg
     6661 acctcatcct ctggatctag taatgcctgt aaaactagat acatcccgtg cattgctccg
     6721 actgtagctc gaacttggtt gcattcaacg ctcaatccat atttcttttg ataaaaatct
     6781 acgaccgttg acaagaattc tttgctgcca tcaggcgcag tataattagt agcaccattt
     6841 tttacatctg tataagctgc ttcaataatc gtcggatcag tgatcaagtc aggatcacca
     6901 atcgataaat cgataatatc ctctttttcc tttgccaaaa tagcaatatt catcaacaga
     6961 ttttctgctg gttgctgaaa ccggcgtgca atcttacttt tgtccatctt acttcctcca
     7021 tctttgttac aacactttgt tcaagaaatc ctgtgttcga ctattttgcg gattttcaaa
     7081 aatctctttc ggtgttccct cttcttgaat gatgccgtca gccataaaaa tcacacgatc
     7141 ggcaacttct ttagcaaaac ccatttcatg agtgaccacg accatcgtca tccctttttt
     7201 agctaaatcc tgcatgactg ccaacacttc accgaccatt tcaggatcaa gtgcagatgt
     7261 aggttcatcg aaaagcatca catcaggatt cattgctaat gctcgagcaa ttgctacccg
     7321 ttgttgctgt cccccagata aactggtagg ataactgtcc actttttcct ttagaccaac
     7381 ttggttcaat aatgatagag catgttcttt tgctttttta gggtcttctt ttttgatttt
     7441 tacaggtgtg atggtcaaat tttcgagtac attcatatgg ggaaacaaat taaaactttg
     7501 aaataccatt cccatttttt gacgaatttg attgatatcg ctgtcagggg ctgtaatgtt
     7561 ttgtccctta aaaaagattt caccagaagt tggttcttcc agcagattca tacaacgtag
     7621 aattgtactt ttcccacttc ctgaagggcc aatgatcacc actacttctc cttctttcac
     7681 agaaagatca attgatttta agacttcatt ctcgccaaaa ctttttgaca gtttttttac
     7741 attaagcatt cagacccatt ctcctttcaa ctaaagctaa tatacgggaa atactgaagg
     7801 tcaacacgaa ataaatcaaa gagaccaaca catatggtag gaatggtttg aagctagcac
     7861 cttgtaccac gcccgtttga aagatcaatt ccgatacccc aatgaccgaa acaactgagg
     7921 actctttgat gatcgtgaca aactcatttc ctagagctgg aagaatattt ttgattgctt
     7981 gtgggaagat gatgaaacgc atggcttctt tctggtccat tcctaaagaa cgtgcagctt
     8041 ccatctgtcc tttgttgacc gcattgatcc cggcacggac aatttcggct acataagcag
     8101 cactattcaa cgagacagca atacaacctg ctaccagttt cgatagatcc aatcccagta
     8161 ctcctgttcc gaagtaaacg ataaagattt gaactaagag aggtgttccc cgtacgtatt
     8221 cgatataaat aatggctaat gcctttaaaa atttattctt acccaatttc atcagtgcaa
     8281 gcaaactacc aaagaccatt ccaaacaata cacccacaaa agctaataaa atagtgtatt
     8341 ttgttccaat caaataatac ggaccgtatt tttgaagaaa ggactgatca tcttggaaca
     8401 tcaattgatt cgcttgtttt tgataatctt tcagcaagcc gtcgtcattg atcgttttga
     8461 ttgacgcatt gacatgattt ttcagttctg gtgtattttt tgacatcgca atagctgtgg
     8521 ttttttcgcc ttgcgcaaat tttactggag tcaaagctac tttcttattc tgttgggcat
     8581 atgcttctgc aactggttgt tccagcacaa caccttcaac tttttcattc agcaaattat
     8641 tgatcagatc aggaactttt tgcagggagg tcaactctgc accagtcagt tcatttttca
     8701 ctaactcctc ttgtgtggtt tgcttttgcg ccccaacggc taagccagaa aagtcggctg
     8761 tcgtcttaaa tttatttttg tcctctttac gaacgagtac tttttgctgt accacgaaat
     8821 atgggtcaga aaagtcaact tcttgtaaac gttcttcagt caccgccata ccagatataa
     8881 ctaaatcgat cttgccagtt ttcaacgcac ctaataatgc atcaaaacca agttcttcta
     8941 cttttagttt cacacccata tcctttgcga tcttttcggc aatggataca tcaaaaccaa
     9001 cgattttatc cttaccatcc acattcgcat ggaattcata gggcgcatag tctgcagata
     9061 ggccaacgac taattctcca ctttgtttta tccgctcgta aatttcatct ttttctgcac
     9121 cttgtacttt tactggtaaa aaaaccagta gaaacatcag cgccactaat aatatgttta
     9181 ttcctttttt catccatttg gccacctttg aataataatg taatcagaaa agctataaaa
     9241 gaataaatat tcataaataa cttgctaata tactagcata actattataa taaaaaaaga
     9301 ttaatttaaa aaaataatgg aagaagtttt ttatatgaca aaaaatttaa ttgttcgcgg
     9361 tgcccctcaa gagtatgaaa taaaagaaaa cgcatgggat tcactccctg aacaccttta
     9421 caaaaggcat attgacaacg ttttgattct ccatggaaaa acatcttgga aagcagtaaa
     9481 agatgttttt ccagatttat caataacaaa aatttttgaa tattatggtg gagaatgtac
     9541 cgatccactt gctgacagtt ttgtttcttt atgcaaaaag aaggatttca atggtattat
     9601 tgcagtcggc ggcgggaaag tagcagatct aggaaaattg attgcctata agacaaactt
     9661 accaatcatc atcttaccta ccttagctgc gacgtgcgca gcttatactc cacttagtgt
     9721 ggtctataat gaagaaggag tgatgcttcg ctatgatatg ttccctaaag caaatgattt
     9781 agtgttgatc gatccaaaga tcattttgca ctcccctcgc gagatgatga ttgccggtat
     9841 tggtgacacc ttagcaaaat ggtatgaatc tcacgcaatc atctctcagt taactatcaa
     9901 gcctatcgaa gtccaagtag cggaatttgc agcgttacga tgccgcgaaa tccttttaga
     9961 gaaaagtgca gaggcaatgg aagcgatgga ccagcaaaaa atcaatcaag cttttatcga
    10021 tgtcgtggaa acaaacattt tattggctgg tatggttggt gggtttggcg atgactacgg
    10081 ccgaacagct ggagctcatt ccattcacga tgcgatgacc gtactccctg atagccatca
    10141 gcaacttcat ggaaacaaag ttgcatacgg tatccttgtc cagctgatga tcgaaaataa
    10201 attagcagaa atcgaacacc tactgccgtt ttatcatcac ttgaacttac caacgagttt
    10261 agctgatatg ggtttacatt taacagaaag tgactatcaa gccgtagctg aacgtgcatg
    10321 tttacctgat gaaatgatcc acttcatgaa agaaacgatc acaccagcac tggttgtcca
    10381 gaaaatgaaa gagttagaac aagtgacaca gggatagtta gccccttata ccatggcagt
    10441 cagctttttt atggatcggc tcaatggtaa aaaaaaagaa gtggaaaaaa ttgacattcc
    10501 acttcttttt tttgcgctaa aattatgatt tcaaggaaat atccgttgct catcttattt
    10561 tagagaacgg aggtgctaag tatgctttat actgaagtat ttttagctgg attcttcttt
    10621 ttaacattaa gcgtcttttt tgatcctcca atcggttatt tctatgaaat ttctatgaag
    10681 ttgattgaat tatgagcgct tttttagtat agttaagctt gtgtaaaaat tataaaagag
    10741 gaagtatttt atgaataaat ggaaaaattg gttattgggc tctgggcttg tcacgatcat
    10801 gcttttcttg tccggttgtg tccgcatgga ttcaaatggt aaccctgaca cttcaggaat
    10861 catttaccgt atattggttc acccaatggg acaagcgatt acatatttag tggaaaactt
    10921 taattggtcc tacggctggg ctgttatctt gatgacagtc atcgttcgta tcatcatttt
    10981 gccattaggt attagtcaat cgaaaaaaac aatgaagatg caagcattga aaccacaagt
    11041 tgaagctacg caacaaaaat tgaaagctgc gacaactcga gaagaacaaa tggctgcaca
    11101 agctgaaatg caacaagttt atcgtgaaaa tggcttaagt atgactggtg gtatcggttg
    11161 tttaccgctt ctgatccaaa tgccgatctt ttctgcgcta tatttcactg ctcgctatac
    11221 agaaggcatc cgtgaatcaa ccttttacgg gatcgaccta ggtaacccta gcttagtttt
    11281 agtagcgatt gccggtattg cttatttact tcaaggctat atctcaacta tcggtatccc
    11341 tgaagaacaa aagaaaacga tgcgtacgat gttgatcgta tcacccgcta tgatcgtctt
    11401 catgtcgatc agtgccccag ctggtgtcac tctttactgg gtagtcggtg ggatctttag
    11461 ttgtctacaa acattcatta cgaatgtcat catgaaacca agattaaaag cgcaagttgc
    11521 ggaagaatta aaacaaaatc cgccaaaaca agtggtgacg cctcgtaaag atgtcacacc
    11581 tgctgaacct gcgaagcaaa caaaaaaaca agtacaagca ccaaaaaaca atgcgaagaa
    11641 tggtcggaat gctggtaaac agcaaaagcg taaatagtta tttgtaacta gcttcttcga
    11701 tcgacccaga tcatttctga ttttatgacg ataaaagcca acgtactgtg aaatatagaa
    11761 aactatattt cgcggtacgt tggctttttt atacacggcc tctttataca tccgtattta
    11821 tatccagttg tatcaccagt caagaagttg tgcttgatta ttcttcttca tcgtctccca
    11881 tgccagcaat cacttggttc actgaaacga tactctcacg acccgaacca ataaaatctg
    11941 gttctgctcc aatcatttgc gaattgatga attcgatgac catcggcaaa ttcatgccag
    12001 catatagttc gatcgtttca ccttccatga ttagcttgga aacggtattt gcaggtgtcc
    12061 cacccaacag atcgcaaagc actacgtaat ctttcaatgg tgctgtaacg gagaggaatc
    12121 tttctttata cgtgtccgcg ccatcttcag gcaataaagg gatcgtatag atattttctt
    12181 gtggtcccat gatcatttct gtactgtttt ttagttcttc acaaaattta ccatgactaa
    12241 tcaagactag ttgtctactc atctttccaa ttctccctta tttcgaaatc acacctaatg
    12301 cagataatgc tacacatacg atcaatacga tgaatattgc tcgagtaggt gtcatatttt
    12361 tcttacctaa catccaataa acgccaccaa cgattccagc tggtaaaagt ttaggtaaga
    12421 tcatatcgat atagttttgc atattcaacg ttacatcccc aatcgttggt gcccatgaga
    12481 atttaacatt gatcatggtt gctactaacg ctccaaccat gaaaactcct agtaaagtag
    12541 ccgcatctgt caatgcagtc aagcgtgatt gcatcgtggt aactagagaa atcccttctt
    12601 tgtaggcaaa ttctagttgt ttccaacgaa agaccatgac agcgacctga gcaaggatcc
    12661 agataaggat acctactgga ttaccatctg ttgccatatt tgcggctaaa gctccaaata
    12721 tggtcgggat caatgccgca aatacagaat caccaattgc agcgaaggaa cccatcaatc
    12781 ctgctttcat cccagaaaca gtttcctttg aagagactcc ttctttttct tcgatagcaa
    12841 gatcgatccc agtaatgatc gtgttaaaga aattagacgt attaaaaaat tgcgtatgtg
    12901 ttttcatcat ttcttttaat tctggtgtat catcaccata gatttttcta agttgtggca
    12961 agatagtata cagataacct gaaccttgca ttctttcata gttccaacct aattggaaag
    13021 taaacaaact tctacgattg atctgcttaa aatcttcttt ggtcaattta taattagatt
    13081 tcgtcatctt cgatttcccc ctcatcagat tgtgattgct gacttactac tggatgttgg
    13141 tcattctttt gatcattctt ataactgatc gttgccagtg ctagaccaat caatgccaca
    13201 gcaagcatcg gtaatgaatt gaatgttcct gggaaatctt tgaccacgct agccacactt
    13261 gttcctaaca attggacatt cgagaacact gttgctaata acgctgtgat cgtaaatcct
    13321 aaaatcagat aagaaatgtg tttctttact ggtaggtagc gtagtaaaat tgcaaaccca
    13381 actgctggta agactgcccc tgcaacagat aagccatctc ctagccattt taggtcgccg
    13441 tttaaaacag ccacaacatt ttggactaag ccaccaccga atgctagggc caagaataca
    13501 ggaaccattc tggaaagtgc ccaaggtaat gcccccatca agaagttccg ttcaattgct
    13561 ttgtaattca ttgtttccac atgttgatcg atccgatgtg caaaatacgt attcgcaaaa
    13621 cgtgccaaaa tatctaattg gatcatcaaa cttgctacag gaaccgcgat ggccgcaatc
    13681 gcttgttctg ggttcatccc tagagaaatg gagaaagctg ttgctaagat cgttccagag
    13741 ttggcatcga tttttgatgc accaccaaat gtccctaccc ctaaaacagt caactgcata
    13801 cttccaccaa tgattaaacc tgcttttaaa tcacccatta ctaacccagc atataatcct
    13861 gcaaaaactg gggcgctcat tgaagaataa atctgtaatt catctaaaat ttgataccct
    13921 gcatataatg ttaataataa tatctgccac cattgaatat ccatgaatca ttcctcctat
    13981 ttgattaaaa gactcataaa gtcttctgct ttgtcatttg gcaccatttg agcgatcaaa
    14041 tgaacgccaa gctgatctaa tttcttgaag acctctacat ccgcatctac gacattgatt
    14101 gattttgtga tggaacgtgt ttcatctgtt tgtgacatat tgccgacatt taattctttg
    14161 atcggtacac ctttttctac tagcgctaag atacgatctg gttttttcgc tacgatcagc
    14221 actcgttgag aatcatattt acctgctaaa atatttgcgg ctgctttttc aataggtaaa
    14281 atacttaatt tgactccagc tggcgttgcc aactttaacc cacttttttc gatcgcattt
    14341 gttgctacct catcgtctac gaccatgatc cgaccaatat tcaactttgt cgtccaaaga
    14401 tttgccactt gtccatgtat caaacgtcca tcaattctta ctccaataat tgccatcatc
    14461 cattctcctt ttctaacttt tttctattgg ttctttgaca aaatctataa cttcactgaa
    14521 tctaccttct gtttcaaaga taacgatttc attttgtcct tttctaaata aaccctcggg
    14581 aacaaacagt gaaagaattg gtcctacttc ccaaaaacga ccaatattcg tgccattcac
    14641 ccaaacgatc cctttcccaa actcactaag gtccaaataa gtatcaccga tttctgacag
    14701 ttccgcttca aagcggtaaa atgctggttg acctgcttcc catttttttg aaaaatcgat
    14761 gtgttctgta gtctctaaag gtaaacaata ctgtgtccat tctgtgataa aatgaagatc
    14821 gctcatcact cctgtacgta ttccttttct ttgcgtatca gctagcagct tgtgtccata
    14881 gttgacccgt cccatatttt caaataatag atcaatttga tggtcttctt tctcaagtgt
    14941 caatggaatg gtctcaccga tctctgtttg gtattgaatc gcttgctttt cttcatcgag
    15001 aaaaacatgg acccgatcac gtccgtcgat gatccgtaat ttttcattga ccgcatcttt
    15061 tttgatcgtt gttcgataca ataggtaccc agtattctga cccaattctt ccattgtttg
    15121 aggataggtc gagtggatcg gcttgctgat ggtatccagt gttgcgaaaa agctgacttt
    15181 ttctttcaag ttgattcctt ttttgacgat tgtttctttt atccatggtt ccatttgctg
    15241 catttctgga aaattttctg ccatcattcg tttcagagca aaatattttt ccgttggatt
    15301 tccctgctca tccaatggtg catcgtaatc ataggaagtg acttgcggca aatcaatcgc
    15361 gcctctggct gaacagccat tcataaaccc aaaattggtt ccgccatgga acatatagag
    15421 attgatactg ccgatctgta atgcttcttt gactgcatcc gctaattctt ccggatctct
    15481 tttaatgatc ggctctttcc aacgattaaa ccagccatcc caaaattcca tacacatcaa
    15541 cggccatttt ttaccatgtg cttcaaaaaa ctgggccata tctgcaaaat tttcttttgc
    15601 ttttgagcca aaattccctg tcgtaaaaac atcatcgtca atcaaaatgc ctgctcttaa
    15661 tgtggcttgc caaggtccat cagaagtgaa tagcggtaca tcgattccac cttcctccaa
    15721 gtattttttt acttggcgca ggtatgcttt ctcttcacca tacgaaccgt actcattttc
    15781 tacctgcacc attaagacgt tgccaccatg agtgactaga tgaggaacga ttttaggtag
    15841 tagcgtttgg taataccgtt tgacatactt catgaagcga ggatccgctg aacgaatacg
    15901 gcactccttt gtcaatagcc aagcaggcaa gccaccaaat tcccattctg cacaaatata
    15961 aggggctgga cgtacgattg cgtataatcc aagggattgc gctaactcta gaaaatggga
    16021 aagatccaat attttcgaaa aatcataggt tccttcttct ttttcatgga gattccaagg
    16081 cacataggtt tccacagtat taaatccaag cgctttcaaa ttgtgcaatg accgctccca
    16141 attcgtcgga tgcactcgga aataatggat ggcaccagat aaaattttga aaggttgacc
    16201 gtctaacaaa aactcttctt ttatttcaaa tctagtcaag caaatttcct cctcgttttt
    16261 agaaaccgct ttctaatttt ataatcaacg aatctttctg acgttgattt cccccctaaa
    16321 ataatcgact ttcacaggat cgatccatcc tgttttttct tgttgtgcat tcgccatccc
    16381 acaggccatc gcaaacgcaa gtgtttcttc aatcgtatag ctctttgata gagcatgtgc
    16441 taagcctgca atagtcgaat ctcctgaccc cacaggattc accaccgcga ttttaggaat
    16501 cgtaacctga tagaagtgtc tccctacttt agcgattgct ccttttgcgc ctagcgaaag
    16561 aacgatccaa ttgattcctt caaaaagcgg atgatccaat aatgattgga tttcatctaa
    16621 cgaagtagcc gaagaaattg tttggtggac caactctcct aattcttcca aattaggttt
    16681 gatcaaaaat ggtttttcct tgccttccaa gacttcaagc aaggtttgac cagaagtatc
    16741 taacaatact cgtgttcctt ttttattcgt tagggcaagt aactctgcat aataactttt
    16801 agaaaatcct tgaggcagac tacccgaaat cgtaaagata tcagttgttt ccattagtcg
    16861 tgcaaaatgc tcggtaaatt tatgttgctc ttcttccgtc acaacaggac cgctttccaa
    16921 tagttctgtt tgattcactc catgtaaaat agcaatcgaa tctctcgttt cccctttgat
    16981 aggtaaaaat tgatgcgtta tccctgattg atcgagttgt tctttgatat atgctccatg
    17041 ctgcccgccg actaatccag tagctaaaac atcatctgct aataaatgaa ggacacgagc
    17101 aacattcaag ccttttcctc cagccgtttt gttgaccgtt ttgacccgat tgacttggtc
    17161 gatccgtaat tcatccaacg tgtaagcgat gtcaattgaa ggattcatgg taactgtcgt
    17221 gatcattttg ctgtcaccga atcttctttt actttgtcaa accaaggggt ggcgttttca
    17281 tttatcactt tattcaacga ttcgatattt tgtcgccctt gtgttctcaa ccactcaatc
    17341 gcaccttctc taccattttc aacatatgct ttgactccat ctttccaagt cgcacgtccg
    17401 cataacacac cgttgaatgt tgaaccagat tcatgagcaa agatcaatgt ttgttggaac
    17461 aatgcagtcg aaacccctgc actcaagaag ataaatggta acggtgttgc atcactttgt
    17521 tccttgaaga atgccgctgc ttctttttta ctatacgcaa tttcaccatc agaataacct
    17581 tctacatagt tcatattgac tggcacttct actttcaata catccacttt atatttagga
    17641 tcagaaaatt ctttcatcat ctcgatcact ttatgcggtt tgatcttcgc atattctaat
    17701 gaagtgctgt cattggtttt ggcatcatac gaaaccaatt ctagataaaa cggacgcttt
    17761 tcacctgcac attcactgcc taaacgttcg ataaatacat gtttcaaatg attgatctct
    17821 gggtcttcat ctacatcata ataaagcaaa aacttgatcg catcggctgt ttgttccttt
    17881 aatctaagcg tagaccaatc cgctaataaa tcaggcagac gtcctggagt tgtcgcatca
    17941 taacccgttt tttcataggc taaaagtagt cctgcttcct gatgtctggc tttagcagct
    18001 ggcaatccat actctggatc tagtaaaata gcagaagcat aaggcgttaa ttctgctgat
    18061 actaattctt tgaattcagt gatctgttgc gccgttggtt cttttcctaa cgctttgatc
    18121 atctttttca aagcaccgcg ttgatcaatt gccaatgcac tgatgattcc ttcttttgtt
    18181 gataaactct tcatagctgc aattttttct tcattcatcc ttcgtttccc ctttgttcac
    18241 atattcatgg ataatgacac ctttgaccac tcgattgact gtacctgtag gcgatggtgt
    18301 atctggttta ttcgctactt ttatcgacgt atcgagcgca atgatttggg ctgtcatgat
    18361 cataggtaag gcaagatacg catcaggtag gctgatcgaa gcttctaaca aaagacttgt
    18421 acctgtgaaa tcgatgtttc ctggttgggc gatcgcgatg acactcgtgg taatttggtc
    18481 atcttttatt tcttctagaa tatctaagtc ataatttctt gtatacggtt ggttattaac
    18541 aaaaccaata acaagtgttt cgctatttaa aaacgatttt ggtccatggc ggaagcccat
    18601 agaagaatca aagatcgttg ccactttacc ggctgtcaat tccaatattt tcaattgtgc
    18661 ttctctcgtt gcaccagcca aacttcctga accaagatag gcgatacgtt caaatgactt
    18721 aggcaataat ttttggattt cttgatctcg tgaaattaga tcttctccag ttctgatcaa
    18781 ttgttgaaga taactttctt ttttctcaac ggcagtttgg tcgaataccc ataacgcact
    18841 caacaacatg cacgtaaaac ttcctgtcat ggcaaatcct gcatcatttg atttttctgg
    18901 catcagtaaa agataatttc gttcatctgt cgctgcattc tttgctaact gaccttcttt
    18961 agcacaagta atcgtcaaat gaaacaatgt cttgatttgt tcattggcaa tcttgactgt
    19021 tgccacactt tctggactat ttccactacg tgcaaatgac accagtaatg tcggttcatc
    19081 cggaaataga aattcttttg gtgaagacac gatatctgtc gtaccaaagc tatgaaaaat
    19141 aaagtgatcg ttatcaccgt attcatttaa ataaggtact gctgtatccc cgacatattg
    19201 tgatgtgcct gcaccagtaa aaatcactcg aactcttttg ccatttgctg ctttttttat
    19261 ctggtcaaaa aacaatgcta atttttcttg attctgtgct aatatctcag ctgtttcttt
    19321 ccataattgc ggttgctgtt tgatttctcg cgtagttatt tccgcaccca actcttttaa
    19381 tttatccttc gatagttcaa acatgttgtt ttccctctca atcattttcg gacgtggcgg
    19441 atcttataat gaaactggtc tgctctcgct acactatccg tatattcaat aatttcattt
    19501 ttcacattat aggtcgttcg gcttagatat aataccgggg caccatcagc gatacgaagc
    19561 ggatcagcat ctttcttttg aacgatactt gcataaaatt cttcatctgc taattggact
    19621 tgctgttcat aatcctgaga aaacacatca tacaatggct tactttcaag taatggcaga
    19681 tcaagaccaa taaatagttt tgcaggtaaa taagaacgtt ccaccatcaa tggttcgtca
    19741 tccgctaaac gaagtctttt caatttgaaa actgtttcgc ctaaattgat gtttagattc
    19801 ttggcaacct ttttgtttgc ttccatttgc ttgaattcca agatgactgt attaggcacc
    19861 cgtcccaatt ccctcatctc ttctgtaaaa ctatacgctc ccgccagatc agacgactta
    19921 tctgatagat cagacacata tgttcctttt ccgtgtcggc gataaatata cccttgcgtc
    19981 tctaattctt gcaaagcaag gcgaaccgtt gtccgactca ctccataatg ctgacaaagt
    20041 tctctttcag ataacaattt gtcatgaggt gtcatctgtg tttcgatacg ttctttcaat
    20101 acgtcaacta gttgataata taatgatttt cctttacttg agctctccat aaaaatacct
    20161 cttctttact ggttataacc acaacacaag aatacccaca tattcctcat ttgtcaaagg
    20221 gtttttggcg taaaaaagct attataacaa ggtttcaagg taatttagaa ctggtatcaa
    20281 aagatacctc aaaaatacca gttgacaaat aaaaaacaaa aaaaagctga atcttggcaa
    20341 agatt
//
LOCUS       DAYYHS010000052        19776 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000052 DAYYHS010000000
VERSION     DAYYHS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.052"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..503
                     /locus_tag="ACHA6A_001816"
     CDS             21..503
                     /locus_tag="ACHA6A_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970003.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            700..1590
                     /locus_tag="ACHA6A_001817"
     CDS             700..1590
                     /locus_tag="ACHA6A_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8970004.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1916..4390
                     /locus_tag="ACHA6A_001818"
     CDS             1916..4390
                     /locus_tag="ACHA6A_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8970005.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4493..6229)
                     /locus_tag="ACHA6A_001819"
     CDS             complement(4493..6229)
                     /locus_tag="ACHA6A_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8970006.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6226..6930)
                     /locus_tag="ACHA6A_001820"
     CDS             complement(6226..6930)
                     /locus_tag="ACHA6A_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8970007.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7061..7564
                     /locus_tag="ACHA6A_001821"
     CDS             7061..7564
                     /locus_tag="ACHA6A_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8970008.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7524..7862
                     /locus_tag="ACHA6A_001822"
     CDS             7524..7862
                     /locus_tag="ACHA6A_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8970009.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7883..9301
                     /gene="ffh"
                     /locus_tag="ACHA6A_001823"
     CDS             7883..9301
                     /gene="ffh"
                     /locus_tag="ACHA6A_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8970010.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9413..9991
                     /locus_tag="ACHA6A_001824"
     CDS             9413..9991
                     /locus_tag="ACHA6A_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8970011.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9995..10516
                     /locus_tag="ACHA6A_001825"
     CDS             9995..10516
                     /locus_tag="ACHA6A_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8970012.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10595..11149)
                     /locus_tag="ACHA6A_001826"
     CDS             complement(10595..11149)
                     /locus_tag="ACHA6A_001826"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8970013.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11402..11677
                     /gene="rpsP"
                     /locus_tag="ACHA6A_001827"
     CDS             11402..11677
                     /gene="rpsP"
                     /locus_tag="ACHA6A_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8970014.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11689..11940
                     /locus_tag="ACHA6A_001828"
     CDS             11689..11940
                     /locus_tag="ACHA6A_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8970015.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12064..12855
                     /locus_tag="ACHA6A_001829"
     CDS             12064..12855
                     /locus_tag="ACHA6A_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970016.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13025..13546
                     /gene="rimM"
                     /locus_tag="ACHA6A_001830"
     CDS             13025..13546
                     /gene="rimM"
                     /locus_tag="ACHA6A_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8970017.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13536..14297
                     /gene="trmD"
                     /locus_tag="ACHA6A_001831"
     CDS             13536..14297
                     /gene="trmD"
                     /locus_tag="ACHA6A_001831"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8970018.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14433..14780
                     /gene="rplS"
                     /locus_tag="ACHA6A_001832"
     CDS             14433..14780
                     /gene="rplS"
                     /locus_tag="ACHA6A_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8970019.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15090..15332
                     /locus_tag="ACHA6A_001833"
     CDS             15090..15332
                     /locus_tag="ACHA6A_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8970020.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(15420..15566)
                     /locus_tag="ACHA6A_001834"
                     /pseudo
     CDS             complement(15420..15566)
                     /locus_tag="ACHA6A_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15623..15937
                     /locus_tag="ACHA6A_001835"
                     /pseudo
     CDS             15623..15937
                     /locus_tag="ACHA6A_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            15966..17225
                     /locus_tag="ACHA6A_001836"
     CDS             15966..17225
                     /locus_tag="ACHA6A_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8970021.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            17278..18174
                     /locus_tag="ACHA6A_001837"
     CDS             17278..18174
                     /locus_tag="ACHA6A_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970022.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18185..19054
                     /locus_tag="ACHA6A_001838"
     CDS             18185..19054
                     /locus_tag="ACHA6A_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970023.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19064..19739
                     /locus_tag="ACHA6A_001839"
                     /pseudo
     CDS             19064..19739
                     /locus_tag="ACHA6A_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6480 a         3453 c         4101 g         5742 t
ORIGIN      
        1 agtagtttgg gggaacgtta atgagaagaa aagcagtaat cgatcggctt tatttatttt
       61 tttggttttt tcctatcgtt ttgctacctt ttacatgggt aaatgctgtt gtacaaggtg
      121 cggagatatt aggcctgatc caaggaagcg gattagtggt ttctaggaat tttccagctt
      181 tgactgtttt ctatttagtg ataagtgtag gtctcttatt accaaaatct ctgatcaatc
      241 actacaattg gcagctgctt tacttttctt gcttattagt atttacatct atgttttctt
      301 tgctgcctcg cttgattatc ggccctaatg gtttgagcga gagtggattg aaggattcag
      361 cttattcctc catgtatttc ttgacgatca aaccatggtt ctatttagta attgcctcat
      421 ttattcttgc aacatttttt tacttctgga cagaaatcag gataaaaagt gaacgatctg
      481 tatctataaa aaataactat tgatggtgga tcatcagtaa aaaagcatat gagctgcgta
      541 aaatgcatgt ttctcatatg ctttttttat tattatgtaa agaatgcgaa aaacgtttac
      601 atgattttta cataacttaa ctatattttt acagcaacca tacgagggat ttacagtcga
      661 ctgataaact ttagttgtaa ataagaaagg agcaataaaa tgaaaaaagt attgtttact
      721 ctaggtatga tgggaattct tttagccgga tgcggctcag gatcaggaaa tacagatggc
      781 tctgccacac aaaataattc aagtgactca aatgaacaag taaaaatcgt tgcggtaggt
      841 tcgacagcat tacagccgtt agtcgatgca gcacaagaaa gctttgtaca agaaaatcca
      901 aattatcaaa tctctgtaca aggaggagga agcggaactg gtttgagcca agtcgaagct
      961 ggagcagtta ctatcggaaa ctctgatgta tttgcagaag aaagagacgg agttgatgct
     1021 tcaaaattag tggatcataa agtagcagtg gtcgggatgg caccaatcgt caataaagat
     1081 acagatgtca aagatatcac caaacaggaa ttgattgata tctttacagg aaaaatcact
     1141 aactggaagg aagttggcgg gaaagatcaa aaaatcaacg tcgtgaaccg tgcaaacggc
     1201 agcggtaccc gcgcgacatt cgaaaaatgg ggactggatg gtgctacccc tgtgcagtcc
     1261 caagaacaag attcatcagg aacagttcgg cagctggtaa gtcaaacacc aggagcaatc
     1321 agctatttag ctttctcata tctcgatgat tccacacaag cactaagtat cgacggtgta
     1381 gaaccaaaag aagagaatgt agcagataac agctggggaa tctggtcata tgaacatatg
     1441 tacacgaacg gaaaaccttc ccctgaagtc caaaaattct tagactacat gatgacagaa
     1501 gaaatccaag aaggacctgt caaagaattg ggctatttac caatcacaat gatggaagta
     1561 gaacgtgatc acgaaggaaa tatcaaataa ctagcagata aacatgccac ggtagtcgtt
     1621 actattaagg aaaaatcaac tatgtttccc cacatataca tttttccttt aattgtgaaa
     1681 aatagttcct catattttgc cgttttttga gctaataccg agggtcagct gattgaacac
     1741 cgtttattcg aaaaaaagag ctacgacata gaactgtcgt agcttttttt ttgctatatg
     1801 acaggaatga aaggtgtttg caagcttaat gtgaggaaca ctttccatat ctgttctata
     1861 ctggatagga gaagtttgga aatatccaga aaacgaaatc aatcgaggta gccacatgaa
     1921 gaataaatgg ataaatcgaa gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt
     1981 catccgtctt tttccatcgt ataaagaagg attaacggca gatcaagtca aggaacgaat
     2041 agaaaaaggt gcagcaaata attcagtcga tcccacattc aaaacaaatc agcaaatcgt
     2101 attggaaaac atatttacgt atttcaattt gatctttctt atcttagcca ttttgctttg
     2161 tttagtagaa tcctataaga acttgacttt tttaccagtt atcatagcca atacaggaat
     2221 cgccattttt caggaaattc gatccaaaaa gatcttggac gaactaaatg ttttaaatac
     2281 agcaaaagtc aatgtcatac gagacgggat agaacaaaca atagatatag aagaccttgt
     2341 aatagatgac attgttattt tagagactgg acatcaaatt ccagcagatg cagaggtcgt
     2401 ggaagggaaa ctgcaagtta atgaggcttt attgacaggc gaagcagatg aaataaccaa
     2461 agaaataggc gatccattaa tgtccggcag tttcattgtt tccggaaaag cttacgcccg
     2521 attagacaaa gttggagcag attcgtatat ttcccagctg acagtcaaag ccaagacaat
     2581 gggagaagga gaacagtcgg agatgattgc ttctcttaat caattgatca aatggattgg
     2641 gatcatcatt attcctatcg gtatcctgct tttttctcaa agttactttt ttaatggaaa
     2701 cacgatcaaa gaaagtgtcg ttgcaatgga agcagcattg atcggtatga tacctgaagg
     2761 actgtacttg ctgacgacga ttgcattagc attaagtgcg actaaactag caaagcaaag
     2821 agttctttta cacaatatga aaagcattga gacattggca agggtaaatg tcctttgtgt
     2881 agataaaaca ggaacgatca cagaaaataa aatgtctgtt caaaaagtga tcgtctctaa
     2941 aaaacaagaa ataatcagtc agtcagatca attggaagca tgcattgctg actatgcaaa
     3001 agcgatggca ggagacaatg caacaatgga agctgttaaa gcatatttta caaaaaccac
     3061 tgatcaatcc tatcaccatg ttattccttt ttcttctgtc caaaaattca gcagtgtagc
     3121 gttggcagac aaggtgtatg ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc
     3181 ggaatatgcg gaagagtttg tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg
     3241 tgtgtacgat gggattcttg aagaaccagt attgacagaa gcagtcacac cattaggcta
     3301 tattctgatt gctaatccta tcagaaaaga agcaagagcc acatttgatt acttcaaaaa
     3361 acagcaagta gccatcaaag tgatttctgg agataatccg ctcaccgtat ccaatgtagc
     3421 tgtccaagca gggatttcac atgctgatca gtacgttgat gcatctaaat tatcagaaga
     3481 agaatatgaa gcagctgtcg aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa
     3541 gaaaatcttc gttcaattgc taaagcgtcg taacaataca gtagctatga caggtgatgg
     3601 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     3661 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     3721 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     3781 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     3841 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     3901 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     3961 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     4021 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     4081 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     4141 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     4201 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     4261 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     4321 tgaaaaagag caaaagccgt ttagtcgcgt agttaaaaaa tttctatcga tatttaaacg
     4381 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     4441 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     4501 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     4561 catgaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     4621 gccggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     4681 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     4741 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatc agatttttga
     4801 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     4861 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     4921 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     4981 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     5041 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     5101 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     5161 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     5221 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     5281 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     5341 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     5401 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     5461 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtatac gcccgataca
     5521 tcttactcat ttgttcgctc aacgcattga tcgtctgata caattcttcc cattgatcgg
     5581 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     5641 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     5701 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     5761 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     5821 ctggtaatgc gacatataaa agatccccat tcaaagtagt acttttgcgc aaagcgctac
     5881 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     5941 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     6001 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     6061 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     6121 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     6181 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     6241 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     6301 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     6361 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     6421 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     6481 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     6541 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     6601 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     6661 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     6721 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     6781 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     6841 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     6901 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     6961 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     7021 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     7081 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     7141 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     7201 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     7261 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     7321 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     7381 ttcatgcgga acccattcga tttatgacgg gagtttttcc ggaataaaaa aaaatggtgc
     7441 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     7501 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     7561 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     7621 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     7681 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa cttcatcttt
     7741 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     7801 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     7861 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     7921 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     7981 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     8041 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     8101 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     8161 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     8221 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     8281 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     8341 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     8401 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     8461 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     8521 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     8581 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     8641 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     8701 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     8761 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactatga
     8821 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     8881 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     8941 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     9001 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     9061 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     9121 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     9181 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     9241 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     9301 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     9361 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     9421 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     9481 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     9541 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     9601 tattaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     9661 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     9721 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     9781 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     9841 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     9901 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     9961 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
    10021 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
    10081 tccaacaggt aagcaaacca cttatgaagc tttttatgaa acaatcgata cgaccatcat
    10141 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
    10201 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
    10261 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
    10321 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
    10381 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
    10441 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
    10501 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
    10561 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
    10621 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
    10681 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
    10741 cattcaaaaa gacaagtgat tgtcgtagat ggtggtttgc gccaaagccg ccgatcccac
    10801 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
    10861 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
    10921 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
    10981 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    11041 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    11101 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    11161 tccttcaaac tatctataac aatcttacaa aaaataagta actggagcaa ataaaaacac
    11221 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    11281 atcttctatt tcctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    11341 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    11401 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    11461 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    11521 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    11581 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    11641 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    11701 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    11761 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    11821 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    11881 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    11941 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttat taattgaaga
    12001 aatgattatt tggcaaaaca agcaagaaag cgtatcctta taggaggagg gagtgaacag
    12061 ccaatgagaa agaaaatgtt atttattcca tttgctctat tgttaagcgc ttgtagttcg
    12121 gttactcaag aatcagacaa tacacatcta tcaagcagta tatctgaaac tgttaccagt
    12181 ccagaaaaaa gtagtaccaa aacggctacc accactgaac aaacaatggc tacctcaaat
    12241 aatgagaaaa agactgcttt agaccagttg aaagaacaac agccaaatgt tcccatgcct
    12301 ctagatgtac cagtctcttc aggctatctc aatatcgcag cgacacatac aaagcaaggt
    12361 tattctattt tatattacag aacagatcgt cccctcgggt tgaatgctga cgaattgaat
    12421 caagaaacgc ctattgcaac ctatctatat caatatgggt ttgcttctag tcaagaaaca
    12481 atccaagttt tgcagccctt tgagatcgac acaaatggtc agcaagtgga cttaggttct
    12541 cgaatcacag ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga
    12601 aactggcgta ttcgaatccg tgggaataat atagaaggcc aagatccctt actattagct
    12661 aaagaaatcg tagcttattt ggaagaaaac agtctaccag cacctgaaca atttggtaaa
    12721 attactgttg atatgggtga tacgacgaat cgagccgtag aagtaagctg gcaagaaccg
    12781 aaaaatgcct atacgatcac tcatcaagat cctatgtctg cgttgaagat ggcagtttct
    12841 atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat catttaactc caagtcacag
    12901 gaagtaatca aaaatagcac gccgcatttt tgcataattt tggcttaata ccttaaaaga
    12961 aaactagaat tgcctcttca cacatgctaa aataaaacag acaaaaaaga aatggggata
    13021 taagttgact gaatacttga atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga
    13081 agtccgtgtt atctcaacaa cggatttccc agaagaacgt tacaaaaaag gaacggtgtt
    13141 aacactattc caagacggaa aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca
    13201 taaaaatttt gacttattga gttttgaagg acatccttct atcaatgatg tggaaaaata
    13261 tcgggatggc atattaaaag tgtcaaaaga caactctgta gatctagctg aaaatgaatt
    13321 ctattaccat gagatcatcg gtctgcatgt attagaagat ggaactgagt taggtaaagt
    13381 aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc
    13441 tgatatattg cttccatata tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt
    13501 ggtccgtgta gaaattccag aaggactgat tgacgatgaa gattgatgta ttgactttat
    13561 ttccaagaat gtttgaaggt cctatggggg aatccatcat cggtaaggct gtagataaag
    13621 gattattaga tatcaatatc tctaacttcc gcgattattc agataacaaa catcagacag
    13681 tagatgatta tccatacggc ggtggagcag gtatgctgct caaagtccag cccatctatg
    13741 ataacatcca agccattgaa aaagctgcac cggatgtcaa aaaaagagta attcttcttg
    13801 acccagctgg taaacgattc gatcagaaaa tggcagaaga attttcacaa gaagaacact
    13861 tagtattcat ttgtggtcat tatgagggat atgatgaacg tatccgcaca ttagtgacag
    13921 atgaagtttc cttaggagat tatgtcctta ctgggggaga attaggtgcg atggtcatga
    13981 tcgatgcaac agttcggctt ttgccagatg tgttagggaa tcagacttca gctcaaacgg
    14041 attcttattc aaccggttta ttggaacatc cccaatatac tcgcccggca gaatataaag
    14101 ggatgaaagt accagaagta ttgacaaatg gaaatcataa attgattgaa gaatggcaat
    14161 taaaagaatc actaagaagg acctatcaac gtcggcctga tcttttggaa acacttgaat
    14221 ggacacctca aatgacgaaa tttttagaag aaatcaagaa agaagaacaa gaaaaaacag
    14281 cagatgaagc caagtgactt tttctaaaat attctttaca gtaggttgat aatatgttag
    14341 tattttatgg tgagtgcaaa agcacttaac tattacgata ttccgctatg agaaaagctc
    14401 ataagaatat ttggaataag gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa
    14461 aagaacaatt acgttctgac atcccagcgt tccgccctgg tgacactgtg cgtgttcacg
    14521 caaaagttgt tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac
    14581 gccgcggtgc tggaatcagc gaaacttata cagtacgtaa agtttctaac ggagtgggcg
    14641 ttgaacgtac attcccattg catacaccac gtgttgcaaa aatcgaagta gttcgctacg
    14701 gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa gctgctcgta
    14761 tcaaagaaat ccgtcgttaa tcattgttat cagactgaga aagaaccctt ggtattcaag
    14821 ggttcttttt tcttagcatg taatttgaac accctgatcc agtttttttc tttaattatt
    14881 ggtaggggag aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc
    14941 actatgataa tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac
    15001 ataaatggag tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc
    15061 aagaaaaaat gaaagtttga gacacattca tgaatgatgg aaaagtaaaa caagtaccaa
    15121 gttcaacaaa aaagaaaaac atcctattga aagaagtctt gaaacgcttt gaccatggag
    15181 tgacttatac agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt
    15241 atgtcgaaca aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc
    15301 gggcatatca gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa
    15361 gttcaagtat gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc
    15421 tataatacaa agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag
    15481 cgattcgcag ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa
    15541 tcaattggat gcgttacatg aataaagata attctctttt atttgtacga ataaatacca
    15601 aaacgtacct cctaaaatta gattcaaaga agatagcaaa gaggaaaaag atagttctgc
    15661 agcagctata gcggcatgtg gattgctaga actttccaaa caactgcctg tttcagatcc
    15721 tcgtcatttc gattacgaag agatagcaat caaaatcttg gaagaattac agaaaaacta
    15781 tacaacgaaa cgagttaacg gatcaaacgg tctgcttctc catgccgttt atgataaaaa
    15841 cagcttaaaa ggtgtagatg aatgtgtgat ctggggcgat tacttttatg tagaaggaat
    15901 cactcgtcta gctaaaacat ggtactgtta ctggtgatgt ataagagaag aggaggattc
    15961 atataatgaa gcgatggaaa aaaatgatga ttggatcaat agttttagga atgtcactcg
    16021 tatttggtgc ttgtggtaat caagaagaca gtagtgaaga atcagcaaat gaaattaccg
    16081 tttcgacatg gaattatgaa acaacacctg aattcaaagc gctctttgaa gcattcgaaa
    16141 agaaaacggg aatcaaagtg aaagcagtag atattgcttc agatgattat gatacgaaac
    16201 tcaccacgat gctttcttct ggagacacga cagatgtatt gacgatgaaa aatcttttgt
    16261 cgtattcaaa ttatgcttta cgagatcagt tagtcgatca aacagaacga attagagaat
    16321 tagacactga tgctgctgaa gaaacatatg aaatgtatga tatcgatgga aaaacatatg
    16381 cattgccata tcgaacggac ttttgggtac tatattacaa taagaaaatg tttgatgatg
    16441 caggaatcga atatccaaaa aatctaactt gggacgaata tgaagattta gcaaaaaaac
    16501 ttagtaaaaa tgacggacaa gtttacggag catatcaaca tatctggcgt tcgacaatcc
    16561 aagctattgc agctgctcaa aacgaggcga atcttgttga accagattat caatatatga
    16621 ctgattatta tgatcgagca ttgaggatgc aaaaagaagg cgcgcagatg gattttggca
    16681 cggctaaatc aacgaaagta acctatcagt ctcaatttga agaacaaaaa gcagcaatga
    16741 tgtacatggg aacttggtat atggctggca tactcgcaaa caaagaagca agtaagacag
    16801 aagtagaatg gggaattaca gctattcctc agaagaaaaa aggagaatcg gtcacgacgt
    16861 ttggctcacc aacagcattt gctgtcaata aaaactcaaa gaaacaaaaa gcggcgcaag
    16921 aatttattga atttgcagcc agtgaagaag gagcaaaagt attagcagga gtaggagtag
    16981 ttccttcata tcgtacggat gagattgatc agctttattt taatctcgct ggaatgccaa
    17041 cggatgaaat ttctaaaaaa gctttttctc ctgatgaaat caaacttgaa tttccgattg
    17101 atacgtatgg gcctgcaatc gataagattt tacaagaaga atatgactta gttttggttg
    17161 gagatgaaac gccggaaaaa ggaacggtaa atatggagaa acgtgtagct gcggaaaaag
    17221 aataatcatg aaaggagaga agccaagaga taaaacactc ttggtttcgt catcgaaatg
    17281 gagaatagta taaataagaa agctacaaat catttgcgta gaaaacgaac gtggatcgct
    17341 tattcgttta tcgccccgaa tttcattggt tttttcgttt ttacattaat accaatcctg
    17401 ttttcgcttg tactagcatt tatgaaatgg gattcttttt caacgccaga atttgctgga
    17461 ctagataact ttcgtaaaat ggccaacgat gagacatttt ggatttcttt gaaaaatact
    17521 tttttatata ccgctgctgt agtgcctggc acacttgttt gttcgttagg gctagcttta
    17581 ctactaaatc agaaaattag agggatgaag ctttttcgta cgatattttt ctttccgtat
    17641 gttacttcct tagtagcaat tgctgttgtt tggaatatgt tgtttcatcc aacagtggga
    17701 cctattaata tctttttaag acgatttatt gaacatcctc caggttggac ttcaagttca
    17761 aattgggcgc tttgggcaat tgtaattgtc agtatttggc gagggatggg ttattacatg
    17821 attctatacc ttgcaggact acaaagtatc caaaaagaac tatatgaagc gtcttcgcta
    17881 gatggagcaa accgttggaa acaatttttg aatgtgacat taccttcgtt gagaccaaca
    17941 accttctttg taacgatcat gcttgtcatc aattgtttca aggtgtttga ccttgtgcaa
    18001 gtgatgacgg atggcggccc tggtcgagcg acaaatgtgc tggtttacca aatctacaat
    18061 gaagcattca aaaaatttga ttttggttat gcttcagcta tttcacttgt tttgttcgta
    18121 attgttttgg gcgttacggt tgttcaattt tcttggaata agcgtcgtac ttaggaggtg
    18181 tgatatggaa gagagtgttc gaaatgaaag aaaaaaagca aaaattcgtt caaaaaacat
    18241 gccctttatt caatggttgg gtatgattct gctgattgta ttagctttat tgacgatcat
    18301 tcctttttta tggatggttt ctgcttcatt caaagcaaat aatgaagtgt taacgattcc
    18361 gattcaatgg atccctaaaa cttggcattt agagaattat ctggttattt ggtctcgtat
    18421 tcctttagtg acatttttta agaatacagc ttttttaagc gttgtgatca cgttgattca
    18481 gttgtttact tctagttttg ctgtttacgg gttttctgaa ctaaatttta aagggcgcga
    18541 tattttattt ttagcatata tcggcacgat tgctgtccct tggcagtctt atatgatccc
    18601 gcagttcatt atgatgagaa aagtgggttt aacagatacg ttgtggtcgt tggtgctatt
    18661 acaagcgttc aatgcttttg gtgtgttctt gttgaagcaa tattacagta gtatcccaga
    18721 ttctttatgt gagagtgcgc ggatcgatgg cttatcagag tgggaaatct atcgcaagat
    18781 cattttacct cttacaaaac cggcgattgc cagtttgacg atcatcacat tcgttaatac
    18841 atggaacgat tatatggggc cattcattta tttgtcttct acggaaaata aaacaatcca
    18901 gttagggttg aaaatgttta ttgggctatt tgatgcagag tacgcattaa tcatggctgc
    18961 ctcagttgtt tctgttcttc ctgttgcttt tatattttta gcgatgcaaa aatattttgt
    19021 agaagggatc gcttcatcag gaatgaagga ataggaggtg attatgaaag agcagcgttt
    19081 tgacaaaaat gtttatatgg aattctttta ttggttgtat caatcattgg caagtagcct
    19141 ttgtttttgg tgtgtcaata tcttgtatat agcagctgcg ttccttttgt cgatagatgg
    19201 aaggaatgtc ttttccttta ttttttcttt actttttatt ggtccaggtt gcatcgtgat
    19261 gataggtatg acagaagaat tagcaaaaga aaaaagtttt tcaccagtac gtttgttcat
    19321 aaagacgttt cgccaatttt ggctaaaggg atttttttat tggctatttg cttggatagt
    19381 tagtgtgata atgatttttg attgtttctt ttttatacgt ttttcttatg gaaaatggtt
    19441 gattccgttg tttgttttac ttgcttgttt tacttgcttg tttatcagtt tcctttagca
    19501 tcaactgctg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa gttctacgga
    19561 tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt ttactattga
    19621 caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta ggtccttcta
    19681 tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg gatctgtaga
    19741 ctttataaat tgaaactttc aattaagaat gacagt
//
LOCUS       DAYYHS010000053        19687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000053 DAYYHS010000000
VERSION     DAYYHS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.053"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(50..484)
                     /locus_tag="ACHA6A_001840"
     CDS             complement(50..484)
                     /locus_tag="ACHA6A_001840"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8970024.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(716..1426)
                     /locus_tag="ACHA6A_001841"
     CDS             complement(716..1426)
                     /locus_tag="ACHA6A_001841"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8970025.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1416..2276)
                     /locus_tag="ACHA6A_001842"
     CDS             complement(1416..2276)
                     /locus_tag="ACHA6A_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8970026.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2281..2919)
                     /locus_tag="ACHA6A_001843"
     CDS             complement(2281..2919)
                     /locus_tag="ACHA6A_001843"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8970027.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(2934..3233)
                     /locus_tag="ACHA6A_001844"
     CDS             complement(2934..3233)
                     /locus_tag="ACHA6A_001844"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8970028.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3276..4760)
                     /locus_tag="ACHA6A_001845"
     CDS             complement(3276..4760)
                     /locus_tag="ACHA6A_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8970029.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(4780..5241)
                     /locus_tag="ACHA6A_001846"
     CDS             complement(4780..5241)
                     /locus_tag="ACHA6A_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8970030.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5259..6326)
                     /gene="ulaG"
                     /locus_tag="ACHA6A_001847"
     CDS             complement(5259..6326)
                     /gene="ulaG"
                     /locus_tag="ACHA6A_001847"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8970031.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6544..7314
                     /locus_tag="ACHA6A_001848"
     CDS             6544..7314
                     /locus_tag="ACHA6A_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8970032.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7587..8414)
                     /locus_tag="ACHA6A_001849"
     CDS             complement(7587..8414)
                     /locus_tag="ACHA6A_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970033.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            8982..10217
                     /locus_tag="ACHA6A_001850"
     CDS             8982..10217
                     /locus_tag="ACHA6A_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8970034.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11216..11476)
                     /locus_tag="ACHA6A_001851"
     CDS             complement(11216..11476)
                     /locus_tag="ACHA6A_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8970035.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11503..12804)
                     /locus_tag="ACHA6A_001852"
     CDS             complement(11503..12804)
                     /locus_tag="ACHA6A_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8970036.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(13096..13554)
                     /locus_tag="ACHA6A_001853"
     CDS             complement(13096..13554)
                     /locus_tag="ACHA6A_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8970037.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(13567..14898)
                     /locus_tag="ACHA6A_001854"
     CDS             complement(13567..14898)
                     /locus_tag="ACHA6A_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8970038.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(14909..16351)
                     /locus_tag="ACHA6A_001855"
     CDS             complement(14909..16351)
                     /locus_tag="ACHA6A_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8970039.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(16338..17102)
                     /locus_tag="ACHA6A_001856"
     CDS             complement(16338..17102)
                     /locus_tag="ACHA6A_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8970040.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(17206..18081)
                     /locus_tag="ACHA6A_001857"
     CDS             complement(17206..18081)
                     /locus_tag="ACHA6A_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8970041.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(18336..18420)
                     /locus_tag="ACHA6A_001858"
     tRNA            complement(18336..18420)
                     /locus_tag="ACHA6A_001858"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18384..18386),aa:Leu,seq:caa)
     gene            complement(18438..18509)
                     /locus_tag="ACHA6A_001859"
     tRNA            complement(18438..18509)
                     /locus_tag="ACHA6A_001859"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18475..18477),aa:Gln,seq:ttg)
     gene            complement(18519..18591)
                     /locus_tag="ACHA6A_001860"
     tRNA            complement(18519..18591)
                     /locus_tag="ACHA6A_001860"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18556..18558),aa:His,seq:gtg)
     gene            complement(18653..18737)
                     /locus_tag="ACHA6A_001861"
     tRNA            complement(18653..18737)
                     /locus_tag="ACHA6A_001861"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18701..18703),aa:Leu,seq:caa)
     gene            complement(18758..18828)
                     /locus_tag="ACHA6A_001862"
     tRNA            complement(18758..18828)
                     /locus_tag="ACHA6A_001862"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18794..18796),aa:Cys,seq:gca)
     gene            complement(18874..18945)
                     /locus_tag="ACHA6A_001863"
     tRNA            complement(18874..18945)
                     /locus_tag="ACHA6A_001863"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18911..18913),aa:Gln,seq:ttg)
     gene            complement(18957..19029)
                     /locus_tag="ACHA6A_001864"
     tRNA            complement(18957..19029)
                     /locus_tag="ACHA6A_001864"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18994..18996),aa:His,seq:gtg)
     gene            complement(19032..19105)
                     /locus_tag="ACHA6A_001865"
     tRNA            complement(19032..19105)
                     /locus_tag="ACHA6A_001865"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19071..19073),aa:Trp,seq:cca)
     gene            complement(19112..19192)
                     /locus_tag="ACHA6A_001866"
     tRNA            complement(19112..19192)
                     /locus_tag="ACHA6A_001866"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19156..19158),aa:Tyr,seq:gta)
     gene            complement(19194..19269)
                     /locus_tag="ACHA6A_001867"
     tRNA            complement(19194..19269)
                     /locus_tag="ACHA6A_001867"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19234..19236),aa:Phe,seq:gaa)
     gene            complement(19305..19377)
                     /locus_tag="ACHA6A_001868"
     tRNA            complement(19305..19377)
                     /locus_tag="ACHA6A_001868"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19342..19344),aa:Asp,seq:gtc)
     gene            complement(19387..19459)
                     /locus_tag="ACHA6A_001869"
     tRNA            complement(19387..19459)
                     /locus_tag="ACHA6A_001869"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19424..19426),aa:Val,seq:tac)
     gene            complement(19475..19546)
                     /locus_tag="ACHA6A_001870"
     tRNA            complement(19475..19546)
                     /locus_tag="ACHA6A_001870"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19511..19513),aa:Glu,seq:ttc)
     gene            complement(19561..19650)
                     /locus_tag="ACHA6A_001871"
     tRNA            complement(19561..19650)
                     /locus_tag="ACHA6A_001871"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19614..19616),aa:Ser,seq:gga)
BASE COUNT         6129 a         4202 c         3115 g         6241 t
ORIGIN      
        1 ttttctaaag aactaagcta gaattttttc ttttgtacgt tattatatgt tatttgtcat
       61 taccaaaaag cacctttttt cttctagtat ctatgacata agaaatacca cccatttcct
      121 ttacttgaac aaatcctact gattcataaa atttttttgt tttctcagta tcatctgtca
      181 ataattcgat ttggactggt ccataactag tgatcttctc caaagcttca ttcaaaagca
      241 ctttgcctat tccttggcgc tgatacctcg gatgaatgag gatatcttga ataaatgcga
      301 tcgtcgcaaa atcagtgatc caacgaacga ttccgataat cttcccttcc ccattacgct
      361 ttatcagaga ttcagattgt tcaaatgctc tagctaacct tgcagtgtct tttgtgtaat
      421 tcgtccaacc aacgctctta tataattcag aaatctcatc gatcataagg gaatcaatat
      481 tcattttttt cttcctttct tatacaattc tccctctaat aatacagttc tttgactatt
      541 taatctatca catataactc tcctgtaaag attctctcga ttccttaaaa ttcctctttc
      601 cttttagaat gcatactttt tgataaataa aaaggttgtg ataaattttc ttcacaacct
      661 ctgtttctct ctatcaagtg ttatctttta attttctacc cttaccctag catctctatt
      721 gcccgtagta tgcatacttt ccatgtttgc gaaaataatg tttatctagt aaatattgcg
      781 ggatttcaca tatattgggg ttgatcagct cgttttcttt ggccatcaga caaatttcat
      841 ctaagatcaa actgttttct actgctttca taggtgaatc cccccatgtg aacggtccat
      901 gtccatagac aagtactcca gggacttcgt ttggatctaa ttttctttcc ttaaacgtct
      961 cgacaatcac attccctgta tgaacttcgt aagcttctct tacttcttct ttcgttaatt
     1021 ggcgtgtaca aggaacctta ccataaaaag catcagcatg agtcgtgcca taagctggca
     1081 aatcgcgtcc tgcttgcgcc cacatgacgg agtgcgtgga atgagtatga gtaatagcag
     1141 ttatatcttc aaaagtttga tacaaaacaa catgtgtggg gagatctgaa gatggtttta
     1201 agctatcttc ttctattata tttcccgaca aatctgttac caccatttga tctgcttgca
     1261 tgcattcata tctgacaccg cttggcttaa tgacgatgac tcctagctca cggtccactt
     1321 cactcacatt gccccaagtc aatttgacta atccgtatcg cggcaatgcc agatttgctt
     1381 caaggacacg ttgcttcatt gctgcaattt tttcttcaca agacatcata gccagcctcc
     1441 ttcaatttag aacttaagta gtcttttgcc tgctggattt ctcttcgaaa gtcttggctt
     1501 ttttcactcc acatttcaat cagaaatgtt ccatcataat ctagacgttt caaatgtctt
     1561 aataatccaa gaaaatcaac acaaccgcta ccaaaaggaa catcacgaaa ctggcctgaa
     1621 tattcttttg ttacagggta agtatctttt aagtggatag cagtgatata atcgatccct
     1681 ttctcaagtt ctcgagccgg attattttcc ggccatgcgg acaaattgcc taggtctgga
     1741 tatacttgta gaaaaggtga atgaatctgt tctttgattt ctaaaaattt tgaaatggag
     1801 ttcataaatg gatcatccat gatttccacg gaaagaatga tgccatattt agaagcttcc
     1861 tttacgcttt cttttattcc ttcgataaag tcttctctgg aattcattga tttctcttca
     1921 taataaacat cgtatccagc tatttggatt actttgacgc ttagattatg cgccaaatgg
     1981 attgcttttt tcattagttg tttcgctgct tttttctttt ctggatcagc agaaccaaac
     2041 ggaaatcgcc gatgcgcact taaacaaatc gaatggatac gtacaccgga gctgaacatc
     2101 tcttctctca gctcagctat cttttcttca gaccaatcta atcgtgctaa ccgctcatcc
     2161 gtctcatcaa tcgacatctc gataaaatcg aagtccattt cttttacaag ggaaaaacgt
     2221 tctttccatg aaatatcttt gggtaacgct ttttcataaa tcccaatttg agccatttcc
     2281 ttacccccag atacgaatga tctcatcttt gaagttttgt gcggccttca tcggatcatt
     2341 tgctgctgta ataccacgac cagtgataaa ggtatacaca tcgatccctt caaataagtg
     2401 taatgtgtgt ggatttaatc caccagttac agaaacatta aagcctagtt caatcagttt
     2461 tttgatcttc gataaatctt tttcacccca agtttcacca gataacagcg catcacggct
     2521 ttgatggtag attacctgcc ggataccgat gttatgccaa tccatggctt gttcaaaagt
     2581 ccaattgccg tagagttcta cttgcaattc acctatttct ttttgtgctg cctccatagt
     2641 tggaatcgtt gcacaacaaa tgactgtcat ccagtctgct cctgcttttg caacattact
     2701 cgccactgtt cctccagcgt ctgcacattt cgtatcagcg actagttttt tatctgggaa
     2761 catactgcga atacagcgaa tcgctttttg tccttcctgt aagcaaagaa ttgtgcccac
     2821 ttcaataata tctactacat caccaacttt cataacatct cctaatgcat gttctaagtc
     2881 attgtgatct aacgcaattt gcaaattcgg cctgctcatc ttagttcctt cctttacttg
     2941 tttttttggt accactttgt ttcagcaaat ttttcactga tttctttgac tgacatcaca
     3001 tttttgatac cgataactgt aactcctttt ttctctgcct gagtaaacat attcaaaaaa
     3061 ttgactggcg taaaaactac atcgtattgg ccagctgaac tttttccctc tgaaatggca
     3121 caatgatgga ttttggtaat tggtacgttt agttctttca ttgctttttc cacacttctc
     3181 atcatcatca agcttgttcc tgatccattt gcacacgata ctaaaattct catcttaatc
     3241 cctccattat gagtgttgtc taatcagatt ttcttttata ctctctgctt ttctgcatat
     3301 gtgtcatagt cttctacaat caaaaaataa ccatcaggat ttttccgata ttgtaactgc
     3361 ggaatagcca ataaaccaat gacaactacg gcaattccag cataacctag gactttcatg
     3421 attactgtaa acaatggcca agctgttgcc cagtcgaaca tcccgatata accaccgtat
     3481 gttgacatcc caatccatgt agcgaataat gctgaaccgc ctacttgtat gattcctgaa
     3541 agaaacggga acaggcaagc ggctttgaat ccacctcggt tattggcata aactgctata
     3601 acggcattat caaagaacaa cggaatgaaa ccggctatga cgatcgttgg tgatttcaat
     3661 aaaatcagta aaccaatcat caaaaactgt cccaaagcac caaacaagaa cccaatcgtt
     3721 accgcatttg gcgaaccaaa gccaaaagta gcagctacgt caattcctgg aacagctcct
     3781 ggtaaaatcg tgtttgagat tccttggaaa gattgagtta gttcatcgac gaatgtccga
     3841 acacctaatt gcaagatcgc cagatatacc gcaaaattta aagaagtcgt cataatgtag
     3901 aataagaaac tctgcccttc ttgcatgaat tgcgcttgaa tcaaataatc tttgcctagt
     3961 acaatcagaa tgatcccaaa gaaaaatagc atgagtaacg aagtagcaac catattctca
     4021 ttgaaaatcg acaagaatcc tggtaatttg atatcttcaa tttttttgtc ttgcttgatt
     4081 gatctatttt tcttttcatc ccgctttttc atccactcag ctaattttgc aaatataaag
     4141 attccgaaca tttgctgatg tgccactgca aatccggcac cttctgtcaa ttcctgcgtg
     4201 attcctactg ttaagttaga agctactgtc caataacaac ctaaaatcag tcccataaaa
     4261 atcaatgctt gtatcctacc tagatcagga aagcaaaaca gtaaaatcca aaaagcggta
     4321 gctgcttgtt gaacctgaac gtttccggta gtgaaaacgg aacgcagttt cgtatatttc
     4381 tgaaaacgga ctaataagat atttaaaaca aatgcaatca gtaataaaat catcacatct
     4441 ccaaaggtac ggccaaatgt ctccattaat ccagcatcca cagcattttg tccaaaataa
     4501 ggatctgtaa ccatggcatc caaattgaac cgttccttta gtccaaccaa gattggccga
     4561 aaattattga ccaaaccacc agagccaaca gtcaaaataa agtaaccgac cgttgccttt
     4621 aagaatccag cgatgcattc atgaagcggg cgtttcagca aaatatagcc taataacacg
     4681 ataaaaccaa tcaggtatgc tggctgcgtc aagatatttg ttgcaaaata cgtccaaatg
     4741 cttaacaaaa attcccccat cttttttctc ctctatcaat taattatatt ctgccatcac
     4801 aacttggtaa tcatcgatgg agttcgtaga gatgagacgc tcaaccaatc catctgtcat
     4861 taataattcg gataattttt ggatattttc tagatgttct tctggatctt ttgctgcgag
     4921 tgtaaaaaac aaactagccg ttttctcctc attctcccct tcactttcaa aacggattgg
     4981 ctgtttcaac ttggtaaatg aaatagctgt tccaaaaaca ccatcacttt tttctgaaga
     5041 atgcggcatt gctacttgag gtgcgatcac aatataagga ccgtattttt ctacacaatg
     5101 gatgatctcc tccacatacg tttcatcaat aatttcctta tcgatcaata caagacagct
     5161 ttcggcgata gcttcacgcc aatccgtgat ttctttatct gggtaatgaa tcaattgatg
     5221 gtcaaaaaag tatttcagca agaaactccc cttctttctt ataaaaacga cggaaacggt
     5281 gtatcatttt caatcgagaa gacatcttca aatcctcgat aaaaattaaa ctcaagatca
     5341 tctttgtttg tcggatagat gaatttcccg cctacttgcc agatataagg tttaaatcca
     5401 tacttcaggc gatccttttt gaatttccaa atttcagtga tctcttttgg atcagccata
     5461 aaattagacc aaatatcgta gtggacagga atcactactt ttgcattcaa tgactccgcc
     5521 atgcgcagca tatcaacaga agtaactttg tctgttatcc ctcgtgggtt ttcaccatag
     5581 gcccctaaac agacatcgat tttgtgttca ttaccatgtt tggcaaataa attggagtaa
     5641 tgtgaatctc ctgcatgata aagattccct cctgatgttt cgaataaata gtttactgca
     5701 atttgatcca tctcctgagg catttttcct tttaatacct cttctggatc ttcacaagtg
     5761 accaacgctg tccgatcaaa cgcttctaaa gctactacat tgatgtcttt gattttgact
     5821 gcatcacctg gctttactgt aatcgttttt tcttcaggaa ttccccattt ttgccacgtg
     5881 cgaaccactt cttttggccc aatgaatgtt gcttcaggac aattttgatg aactgctgcg
     5941 gctgtatgga tatctagatg atcggaatgg atatgtgtaa cgattaaagc gtctacattt
     6001 tttatcgcaa aaggatcgat tacgaaaggc tgagtacgta aattaggctg catattttga
     6061 acgccactca tacgcatcat ctgatggcct tttttcatca tgccattccc atgactacgt
     6121 tttcccgtcc cacaccatag atcacataaa atattcgttc cttcatgact tttcagccaa
     6181 attcccgtac agcccagcca ccacatagca aaagttccag gatccactac ttctgtttcg
     6241 atttcttcat tcaagtatgt gccccactca ggaaatgttt ctactaacca cttttctttt
     6301 gttacttcat tgacagtttt actcatttgc ttgctccccc catatcgttg ttccaactta
     6361 caagtaaata ttaatgtgta tcagcatcca tgaaaaccct tttatatgac cgtttgtttt
     6421 ttatatgatt ttctttcagt aatcaatgat tgtttcagta taataaagac agatatgaat
     6481 gtttaaatga acaactagtc aaataaatcc atggatcaag atgtccctca ggaggcaaaa
     6541 gtcatgaaaa actcacttgt caatattgaa aaacgtcaaa aagaaatttt ggaactgctt
     6601 cagcagactg aacaactatc aactttcgaa ttagctgaat cccttgatgt atctattagt
     6661 acgatacgaa gagatttaaa tttattagaa gaaaaaaacg agatcatacg caattacggt
     6721 tattgttctt ataattacaa aaaccagact gattttgatc aatctggtcc tgaaaggatt
     6781 aaacaagcga ttgccagaga agctagtaaa tacattaacg attatgatac gctgtttatc
     6841 aattctagct cgactgcgtt gaaaacactg gattttcttc aatctaacca tttgacagtc
     6901 attacaaaca acgtgaaaat caattactct ccgcacaaag ataattgtag ttatatttta
     6961 actggtggag agatgcgctt tccaaaagaa gcactagtgg gcgacatcgc attaaatacg
     7021 atttcttcag tcaatgcaga catttgtatc attggctgta gcggagtcga tgtagtacat
     7081 ggggtcacaa caaagatatt aaatgaatca acgatcaacg aaatgatggt caacaagaca
     7141 atcaaatgta agattttagt cgctgatcat cgaaaaatcg gactgacatc aaagttcaag
     7201 attgctgata tttcagtctt tgattatttg atcactgata aattttgttc tgaaaaagta
     7261 ttagaggaga tcagaaatac ggggataaaa accattcaag tagattcatt ttaagaacca
     7321 ggacgtaaat atgtaaatag gattatcaaa aaaatgaata acaaatttat cgataaatga
     7381 aaaacacgct acgctcaaat tgataaagga atcccaagat gtatccatcc aatcgatacc
     7441 atcataatct attgcttctt ttataaccgt tatatgttgc ctaaatagga atcaaacaat
     7501 atcgaaaaaa ggaggtatga caaaagtcac acctcctttt ttcgaataaa cggtattcaa
     7561 atcagaacac tgatttcaca accactttat ttcaggtcgt tttcaatcac ctttaccgcg
     7621 taatcatttc cgttgaaacg cgctattttt tttgcgtcca tatatctttc taaataatca
     7681 tcttctccta attgcttatg gcaagaagca atttcataaa gcaagtattc caatgcataa
     7741 gtcgatttat gtttcttatt atactcaata cccttctcac ataattggat tgctttatca
     7801 taatcctcga ttttacggta ataggtggcg atatcataat atacaatgca gaccatcaga
     7861 ttatcaaaat cagtctcact caatatttta agtgtacgct ccacataaac tttcgcaaaa
     7921 tctaattctc cttttgacaa gtagatcatt gccacgtttg cagctgcttg taagtgatgg
     7981 atatctgttt cggtcgtttg ttccaacact tgattaaaat aaaataatgc tgcatctggt
     8041 tttttcgcat aaagatgatc aagcagtcct tgataaaata aaatttgatt taaaagatca
     8101 gaattactta ctttttcctg atcgatcgtt tgtaaaattt tataagattc ttggtatttt
     8161 gatacagaat tcaatgattg aatctcattt atttttttcc tgattttgtc ttcttctgtt
     8221 tctactaata tatcggagat ctcgacattc agctttttgc aaagcgcact taagatattg
     8281 atgcctggcg acatattctt gttttcgata ttgctgacag ttgcttgttc acaaatgcct
     8341 tctgctaatt gtacttgtgt taaccccttt ttctttcggt attcttttag taatgctcca
     8401 tctactttaa tcaaaacaat ttccttccct ttaaaaaatt atttctcctt tatatcatac
     8461 acaaaaaaaa gttctaaaca accaaaaatg tttagaacag aataaaaatt ttaattttaa
     8521 aataatattc aaattaaaca actggcgtat ctgctccttg atttcttttt gtgttatctg
     8581 aataattatc gtttacagtt ataacacata gtgaaaattg tcatggcgag aaagtctaag
     8641 aatattttta aacataacta tttctttctt acgtgtgtat aatgacgaca gcacacctat
     8701 caattaatat aattatttta tttttttatg ttctcatgtt tatttttaat gacacaactt
     8761 ctcgtattta tctggcacaa catataaatc gatcaaaaaa ttgttgcgtg tttttttgaa
     8821 aattggagtt tcaacttgat tacaaaattt aaatttagaa gttgtgaaaa cagcgagaga
     8881 cagtttaata ttcaaaatat acataggcta ctcgaacgct gtgtttttta gtatactagt
     8941 aagaaacgaa gggatagact cattccaata aggaggattt catgaagaca attctttttg
     9001 ctcctgcagt attcaattta gctgagacaa cacgaatgat tgaggttgcc aaagctatga
     9061 aagaccgtta ttactgtgta ttctttggct tttctaaaac ttttgctcct ttgatcacag
     9121 aaagtggctt tgagtatcat ttactagcgc cgatccttac gaaaaaacaa gaagaacaaa
     9181 tcataaaatt tgaccagttg cgtacactta aaaacccttt taccaaagaa atggttgcct
     9241 ctcgtatcac tagtgaacag agtctgattg ctaagtttga gcctcgactg attgtgacag
     9301 gttcgaatgt gacaattttt ttatcggccc gcaataaaaa gattccttta gtatatgtta
     9361 aaccctatgc tttgagtgaa cctttttttc aatcagagca tgcgctgatg ccccacgagt
     9421 tacgtttctt cggcccttta agcaatcccc tttggcactg gacaaaaaag cgtatagtaa
     9481 atacccgatg gatccccaat gccttcaaac acgctgcaca aaaagaaatg cttcctttac
     9541 caaaaacaag tttacaaatg atggatgctg atctaaatct catcacaacg cctaaactat
     9601 ttttggaaga agaacagctg ccggaaaatt atcgaatcgt tggtccaatt tttgctaagc
     9661 tcagtacttc cttgcctgaa gaagtgctaa cttttataca gcgaaagcaa caagaaagaa
     9721 aacgtattat ctatttcgca atgggtagtt caggcaatcc gcgtatgtta cgcaaaattt
     9781 tgttcttctt acaaaaacaa ccggatctag cggttattgc acctattgac tatctgcttg
     9841 atgacattca atctgactca gaagatcttt taataacaga atatctgtct agtcatcttt
     9901 tgcatgaatt cattgatttt tcttttatcc acggcgggga agggaccgtc caaactgcct
     9961 gtgaatccgg caaaccgttt atcggtgtgg ggatgcaatt tgagcaaagc tgtaatattg
    10021 actattgtgt aaaatacgga aacgcaatcg ccctcacaaa accagtgact gaaagaaaac
    10081 tgcagattgc tctgcgcgaa gtttcttcac ccaagattcg tcagcaggca cgcacgctgc
    10141 aagccgtttt tccaataaat ggtccacaac aagcaattca agaaatcgaa accttcctga
    10201 gcaaccaccc aatatagttt caagtagaaa aagctttttt aatagagact catcagtaat
    10261 ttcgctattc gagacccaga cagtgaaaat acaaaaaaag taaatagtgg attgaaaagt
    10321 tagagttact taaattacgg tgtctaaaaa acacaaaatc atccttaaaa taaaagatct
    10381 gacgctatcc aaatgagcat aggtagcgtc tgccatttat tcttagtcaa attctttgac
    10441 acctttctct ggttggatca tctcaacgtt tcttcaaaag aaagaccata ataattaggc
    10501 aaatacattt tcttaaaatg tgtcatatag atctagaagc atcgctttgt agagatttgt
    10561 gccgctagtc ccctcaacaa ttactaaatc aaatgtagga tttaccactt tatctttgat
    10621 aatcaacctc cctcttctgt tgagacaatg atctcgcaat ccgatgcata ctcctatata
    10681 aactgccagc aatccccaca tattgagtag atcatgtcag aatgttctat gtcgtcagca
    10741 acgatacact aaaagtcacg ttcaccatga ctgattgcta ttcccagcac aattatttct
    10801 atacacgttt catgatttga ataaacactc acatcataca tattaccatt catacaccat
    10861 actgcagccc ctactatatg gttgtaagct cccaaatcta tatttgactt gatcactttg
    10921 gtagtagatc tactaattct aaatccattg ctgataaaat ttccattttt tcctccttct
    10981 ttctgccaaa acagttaaaa caataataat ctttatgtaa aaaagggctg tgacaaaagc
    11041 cttgtcacag tcccatattc cgaataaacg gtggtcaaaa gcttgcatct gcggtaataa
    11101 gttcaaccaa acgaaaaata agccaacatt tcacaaaaat ttaagaagca attttcgtaa
    11161 atttcttctt atttcttgaa gctaaccact tctgttacaa ccacttttag aacatttaaa
    11221 gcttcgatgt gacaaaccat ctatgctgct cgatcataaa attgatagca aaaattccac
    11281 aaagtacata tgtcccccat gaaaacaaaa cattcatatg cggtacaatg atctttggaa
    11341 gaatcacgat caaaaatgat gccagtgccc aaatccatcg attgatttga atatgatatg
    11401 tagctagcat attcaatata aggtgactag ccgttaataa aaagataaaa acaaaaaata
    11461 aaatcagcca atacattgtt tcccaccttc taaaaaccaa acttagacag tctggacttc
    11521 gttttctcca cgactttgtt catctgacag catcttttta tcataagctt ttacaaatgg
    11581 ataatagata ataaatgcta tcaatgtgtt gatcatgatc aagataacaa cacgccagtc
    11641 atttcctgct gaaagataac cgccgattgg agctggtaat gtccatgttg gcaaaacagt
    11701 aaatctattg accatttcta atttgactgc aaaataactg ataatagtca aaactaaagg
    11761 aactaagttg aacgggatga acatgtaagg attcaacatc ataggagctc caaaaattat
    11821 gggctcattg atattgaaga taccaggaat aatagaaaaa cgaccaacct ctttcaagaa
    11881 ttttgattta cagaagaata taaacaaaac agataccacg atcgttgctc ctgcaccacc
    11941 aattgttacc gtccattgat aaaactgttc aggagcgata tagggtaatt gggtggcagc
    12001 tgtaccatct gccaatgcct ttgcattctc atccaacatg atcagccaca ttggacgaac
    12061 aacagcccct acaatactca tgccatggat accaaatgac cacaacaaat ggatcaaaaa
    12121 cattggtagt aaaacaccaa gcaaattatt ccctaaaata ttggttaaag gctcgaataa
    12181 cgataataag gcagcgttta catcaaagcc cgcgacgctt cgaatcagcc aagtaattgt
    12241 taaaatgact gcacctggaa tcaatgcctc aaaactacgg gcaacagcag gtggcacttg
    12301 ttctggcatt ttgatagtaa tatttcgatt tttaaagaaa cgatatactt ccactgcaaa
    12361 aatcatagat aagatccccg caaacatccc agatgctccc aaagaactca taggtaagac
    12421 ccagccaaga ggtgcctctt caggagttaa aacgtttaca ggaacttgta acataaagaa
    12481 cgttcctaaa gataatatac cacctgtcac actatctaat ccatatgatt tagctagatc
    12541 cgaacccata acaaaagttg cataaatagc catcaatccc atcgtcatcc taaagggtaa
    12601 aacaatcgtt gccgaataag gggcaataaa ttcattccat ccaggaaacg gtaaattgat
    12661 aaagaccata aaaaatgtcc caatcaaaat caatgacaat gttgaaacaa ccccattacg
    12721 aattgctaat aaatgacgct gtgtaccaat ttttgccatt ggtgggacaa gtttctcttc
    12781 gacccatttc atcagctttt ccactaaaac cactcctttt caataaaata tgaaccattc
    12841 attgcatgac aacgcaaaga atgttcataa ctaattatat aattaagaaa ttttatttca
    12901 atatattctc ttaaataatc aaatttattt ttaagaatcc tgaaaataag ttttctaaat
    12961 cataggagca acaaaaaaat tcgataatta acaaaaatca aaatgattaa atctagtgat
    13021 tgaaacatat catttcaaaa aaattttgta aattcaccca taaatatcac cctatttaac
    13081 ttttttcatc gtacactaaa attcaaaaca actatcttta atctttactt cttggttttg
    13141 ttttaataaa tttccttcaa aaatgcctgg agaaactaca atgatcaccg tatctttttg
    13201 tgcttgctcc aacttcttca aatctataat agctaacagt gtcccttctt ttacctcctg
    13261 gttactttca acccgtatct gaaacggact tccttcgagt tcaactgtat ctaaacccat
    13321 atggatcagt atttttctgc cagattcact ttcgatcgtt atggcatgtc gggtaggaaa
    13381 tatattatcg attattcctt taactggcgc atggatatat ccatcatgat tcgttatagc
    13441 gtatccaact cctatcattt ttgaagaaaa gacctcgtct tttactgctg ttaatggaat
    13501 aatttgacca ctagctacag cttttagaat gtttcttgtt ttaaagaaat gcacttttta
    13561 tcacctctac tttaatgctg gccaatattt tttgtttact ttgatcattt catctagtat
    13621 tttttttgcg actctggcat ttggtacggt ttgattcaat gtaaatgctt gtaaagcttt
    13681 ttgataagaa ccttcaaagt aagcatctac tagtaacttc tctactgcaa cttgtgcctc
    13741 catcaatcct ttatgaaaat cactgattgg ttttcttagt gtaattggct ccacaccagt
    13801 tgcaccaaca tatgccggta tttcaacaac tgcatctgac cgtagattcg gaatcgcccc
    13861 ttgattagga acgattagca taaagcgacg acgatcatca ttcaacaagg aaatagctat
    13921 atcgacgata tattgtccat gctccccaaa attaaagttc aatacttctt ccacaatacc
    13981 gcggcgtatt ttatcagcca tttctttggt atttttctct cgtccatcca tgaccatatt
    14041 agcacgtgta tactctttgt cagtatgctc aacgaccata tcaggatata agtaatactc
    14101 taagtaatta ttcgggatat tacttggaaa atttttcgtg atgactgaca tcatttgaaa
    14161 ggttttttgc caagttttat ctcctatatt aaaatcggcg acttgcattt cttttgttat
    14221 tagtttttca ataatctctg gcataatttc tcgttgcaat tctttatcat aaatcgatgt
    14281 ataccagcca aagtggttta aaccataata cgtaacgatc caattcttac gatcataacc
    14341 gtaattcact gcaatcgttt cttcaattga aatagtcata tcacaggcat taatcatttt
    14401 tacattcgga tatttacgac gaacagcttc agaaacgatc gactctggat ttgtataatt
    14461 caaaatccat gctttggggg catagtcttg tatatgatcg acaatttcca ataaaccttt
    14521 gattgagcgc atcccataag caaatcctcc gagtccacac gtttcttgtc ctactaatcc
    14581 atgtttaagt ggaatctttt cgtccttttc tcgcatctcc aatcctccaa cacgaatttg
    14641 tgagaaaaca aaatcgcatc ctgtaaaagc aagctttgga tcatccgttg cacgaatgcg
    14701 aatatttgaa aaaccttttc ttttcagcat aaatttaaca atcaagtaca tatccatatt
    14761 tctttcttca tcaatatcat ataaacgtat ttcagataat ggcaaccgtt tttcattgtt
    14821 caaaatggct tgaacgattc ctggtgtata cgtactcccg cctccagcta tagtgataat
    14881 ctgttttttc atcatcatct tttcctttct attcttctaa taatttattg aattctgcca
    14941 taacataagt cacttctaag ccgatgataa tttgataatt tttttcagtt ggtttaatga
    15001 tcccgcttat ccctaattgc ttgattcgct gttcctcgac catggtggga tccactacct
    15061 ctacccgtag gcgtgtatta caactataga catccaggat attggctttt cctccaaacg
    15121 cttgtagcat ttttgttgcc atatattcat aatttttact tgctaaccct aactctgttt
    15181 cttctaaggt tacttcattg ccaaactctg ccttagcttc acgtcctaat gtctgaatgt
    15241 ttctcttatt gatcaaataa taaaaagtaa cgtaatacaa ggcaaaaaag aaaattccga
    15301 ttggaagaat caaaatagca ttggtagcga ttcgaaaatt gatgagataa tcaacaatac
    15361 atgctccgaa tgtaaaacca attcgtatat tgaataagta acagacaatc cctgctaaac
    15421 cggcaaaaag tgcatggatc acgtacaaca taggagaaag aaacatgaaa ctaaattcaa
    15481 caggctcagt gatatttgca ataaatgaag ttcctgcacc gcttcccatg attcctttga
    15541 ccatctcttt attttcttta taagctgctt tatagatagc tagacatata gctggtatac
    15601 caaacatcat tactacaaaa aaaactgata aaaacagtcc agcctgagga tctccgttga
    15661 taaagcgtgt catctctcct ctgaaaactt ccttgctagg agatgtatag cttcctaaat
    15721 cgtaataaat atacgtattc aacacatggt gcagtccaaa ggggatcagc aaacgattca
    15781 aaaacataaa tatccccaca cctaatgctc ccatatcaac gagcagtttt gcaaaattat
    15841 caatcccaat ttggatgagc ggccagataa agcccaaaaa aacagctgcg attgtttgta
    15901 gaatcatcac catagtcaat gggaattttt cgcccgcaaa ataagaaaaa actactggta
    15961 atttttgttc tttaaagcga ttatacgtcc atgccgctac taaaccggaa ataattccac
    16021 caaaaatccc catattcact tctgggtcca taatttctaa gccttttttc aaggtagcaa
    16081 tcgctaaaaa accagtaagt gcaggtattc ctttgtcctt atttttgaca aaaccgattg
    16141 caactcctat agcaaataag atatccaagt ttccaaaaac ggcatttcca gctgcaccaa
    16201 ttactgggat gttcagcata tcgtcagctg ataaacgcat gatcaatccc gctaatggca
    16261 tcgctgcaac tggaagtagc atcgctcttc caagcttctg cacatttgat ttaacattca
    16321 tttttattcc tcctctatta aatatcctca tatgttacta actcaatcaa aggatcattt
    16381 ttcacttttt gagcaaatgc tgaatgaatt aaaaattcga cttggtgggt acgaggatac
    16441 acaaaacttg aaccatttaa caattcctga tccaaatacg ctgcgtgaca catgacttcg
    16501 atcgttttat cttctctcaa tatcatgagt aaacttgcca aatattcttc cacactcgta
    16561 ttgatttgtc gttttttttc atcagtttct cctacgacat cgaaacaagg ctcaaaataa
    16621 tcgacatgac gaacttcatc ttttcgttca aagttgctcc gaaccggcaa atcatatttt
    16681 tttgctaatg taacaactac ctcctgattt tcatccaaag tatgagtatg atgatgactg
    16741 tctaaatgag ttggtatgat ccccgcatga taaatttttt gaatctgagc attccattcc
    16801 tgatacagtt gatctttgtc aatgttaaat ggatgctgat aataagataa ggatctgaac
    16861 tctccgcttt cgataagagt atcgacagtt ttgaggagtg gtttgccaca cgtcaatgtt
    16921 aaatggactc ctacacctag cctaggcatt tcctttctta gtttcacagc atgctcgaaa
    16981 cccggcatat ttgccatcaa tgtagttgat gtcaaaattc ctcgctgata agcatccata
    17041 atcccataat tcacaccatg gctatagcca aaatcatccg aattaaaaat cacttttccc
    17101 atttcattct ccttcttttt tctactaaaa taggcgcaac caaataatca tagtccattt
    17161 actatgtgtc atttggttgc gcctatagat agttacgccc ttattttaat agattcttgc
    17221 tagatgaatt gccaaataga tgatttcatt ttcttgaatg gcaagttgaa attgctgttc
    17281 gatgatatct ttaatcttta gtgctgtaca atacgctttg ctatgtttaa atgtttcagt
    17341 aaaaaaacca atattttcta ctggcgtttc ttcctgataa atcaagcgtt taaccaggaa
    17401 tttgatatga accattaagc gatgataagt cagtgattct ttatcataaa cgtttgaata
    17461 ggatttttct agatgggaca aaatcgtggt cgtcatttct actattttta agctctttaa
    17521 actattaggc tctcctagct cagcattaat gatatgcata gtgatgaatc ctacttcatt
    17581 tgaccctaac tcggtttgat atcgatgatt gattcgctcg accacttcta ctgctatatc
    17641 atattcattt ggataaagga atctcatttc tggcaacata attgctgtga catctctatc
    17701 ttctcttttt cgttgaatag caaaataaat atgatctgtc aatgtaatat agatatttga
    17761 atctaatgga gaggagatcc tagctgttat cagttcaatc acttcttccg caaaatccat
    17821 aaattcgatt ggaatctgcg agactatctc actgaatcgt ttgatctttt ctttagaatc
    17881 taatacaaat aatttgtcag catcctcaac atcaaatggc ttactcactt tagcagaaaa
    17941 gccaatccct ttccccatcg caattttttc tattccatcg tcatcgatca atacagcatt
    18001 atgattcagc tttttcatca catacaccgt taattcacct cctctatatg ataaaacaaa
    18061 acaacttgca gactccttca tacatatttt tgtctccaaa caatttttaa agacaaaaaa
    18121 cgacctaaga aagaaccctt tcttaggtcg tgcctaattg aatagtagca ccctacataa
    18181 tttacaaaaa tagtttaaca cataataaaa gcgttatcaa tatctttttt actcgtttta
    18241 tgataatact tgtatcgaat tgatttggag gtaattacat aaaaacaaaa aaaatatagc
    18301 aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc
    18361 acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc
    18421 aaatttaaaa ataataactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt
    18481 cccttgcctt accgcttggc tataccccaa gaataaaggg cgaatgatgg gaatcgaacc
    18541 cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatcataaa
    18601 agatgttatt taatttctca ataaaaaact aagatgaaga catcttagtc aataccggcg
    18661 gccggggtcg aaccggcacg ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc
    18721 aattccgcca cgccggcaag ttgatttatt agtactaagg cggtaaccgg atttgaaccg
    18781 gtgatgaagg ttttgcagac ctctgcctta ccacttggct ataccgccat tatactatta
    18841 tcaatctata aaataaagaa taaattatag aaactggggt agctggattc gaaccaacgc
    18901 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg
    18961 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
    19021 cacattcgcc atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc
    19081 tgttctacct ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac
    19141 cctaaggaac ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc
    19201 gcgggacaga atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc
    19261 taccgcgcca tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat
    19321 cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt
    19381 tcattatgga ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc
    19441 ccagctgagc taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg
    19501 ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta
    19561 cggagagtaa gggattcgaa cccttgagac agtttggcac catctacatg atttccaatc
    19621 atgctccttc ggcctctcgg acaactctcc agagtgaaag aagctcaata ctctttctca
    19681 aaatgag
//
LOCUS       DAYYHS010000054        19631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000054 DAYYHS010000000
VERSION     DAYYHS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.054"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHA6A_001872"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHA6A_001872"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8970042.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACHA6A_001873"
     CDS             complement(1174..1869)
                     /locus_tag="ACHA6A_001873"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8970043.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACHA6A_001874"
     CDS             complement(1882..2598)
                     /locus_tag="ACHA6A_001874"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8970044.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACHA6A_001875"
     CDS             2928..3536
                     /locus_tag="ACHA6A_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8970045.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACHA6A_001876"
     CDS             3861..4184
                     /locus_tag="ACHA6A_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8970046.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACHA6A_001877"
     CDS             4366..5736
                     /locus_tag="ACHA6A_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8970047.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACHA6A_001878"
     CDS             complement(5866..7167)
                     /locus_tag="ACHA6A_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8970048.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACHA6A_001879"
     CDS             complement(7521..9512)
                     /locus_tag="ACHA6A_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8970049.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACHA6A_001880"
     CDS             complement(9656..9922)
                     /locus_tag="ACHA6A_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8970050.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHA6A_001881"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHA6A_001881"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8970051.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHA6A_001882"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHA6A_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8970052.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACHA6A_001883"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACHA6A_001883"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8970053.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACHA6A_001884"
     CDS             complement(14082..14603)
                     /locus_tag="ACHA6A_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8970054.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACHA6A_001885"
     CDS             complement(14606..15448)
                     /locus_tag="ACHA6A_001885"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8970055.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACHA6A_001886"
     CDS             complement(15441..16166)
                     /locus_tag="ACHA6A_001886"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8970056.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACHA6A_001887"
     CDS             16342..16638
                     /locus_tag="ACHA6A_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970057.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACHA6A_001888"
     CDS             17206..18798
                     /locus_tag="ACHA6A_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8970058.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACHA6A_001889"
     CDS             18811..19551
                     /locus_tag="ACHA6A_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8970059.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5618 a         4028 c         3724 g         6261 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaga
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt c
//
LOCUS       DAYYHS010000055        19555 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000055 DAYYHS010000000
VERSION     DAYYHS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.055"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(541..1566)
                     /locus_tag="ACHA6A_001890"
     CDS             complement(541..1566)
                     /locus_tag="ACHA6A_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8970060.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="ACHA6A_001891"
     CDS             1841..3526
                     /locus_tag="ACHA6A_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8970061.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="ACHA6A_001892"
     CDS             3540..4823
                     /locus_tag="ACHA6A_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8970062.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="ACHA6A_001893"
     CDS             4893..5762
                     /locus_tag="ACHA6A_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970063.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="ACHA6A_001894"
     CDS             5759..6595
                     /locus_tag="ACHA6A_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970064.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="ACHA6A_001895"
     CDS             6621..7688
                     /locus_tag="ACHA6A_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8970065.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="ACHA6A_001896"
     CDS             7685..8512
                     /locus_tag="ACHA6A_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8970066.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="ACHA6A_001897"
     CDS             8557..9582
                     /locus_tag="ACHA6A_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8970067.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="ACHA6A_001898"
     CDS             9579..11762
                     /locus_tag="ACHA6A_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8970068.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="ACHA6A_001899"
     CDS             11762..12892
                     /locus_tag="ACHA6A_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8970069.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="ACHA6A_001900"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="ACHA6A_001900"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8970070.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="ACHA6A_001901"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="ACHA6A_001901"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8970071.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="ACHA6A_001902"
     CDS             complement(14378..15064)
                     /locus_tag="ACHA6A_001902"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8970072.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="ACHA6A_001903"
     CDS             15144..16682
                     /locus_tag="ACHA6A_001903"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8970073.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="ACHA6A_001904"
     CDS             16802..17269
                     /locus_tag="ACHA6A_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8970074.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17803..18231
                     /gene="rplM"
                     /locus_tag="ACHA6A_001905"
     CDS             17803..18231
                     /gene="rplM"
                     /locus_tag="ACHA6A_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8970075.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="ACHA6A_001906"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="ACHA6A_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8970076.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..>19555)
                     /locus_tag="ACHA6A_001907"
     CDS             complement(18727..>19555)
                     /locus_tag="ACHA6A_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8970077.1"
                     /translation="ELTKKSKEYANIKIIKGYVRSMFDIAEILNYIEFNRTTKIIQSI
                     TAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDR
                     KSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSV
                     QADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHK
                     LRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         6333 a         3531 c         4149 g         5542 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaattatt tggaaagtgc ttcttttgtt tttaagaaca catttaagaa
    18721 cacatatcaa tcacacttat tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac
    18781 tatatctggt gtgttcacat atatctttgt tgtagaaata tctgagtgtg ttaaagcatt
    18841 tgaaatttga ttcatatctg ctccaccttg tctagctata gttgcataag tatgacggag
    18901 tttgtgtgga gataaatgaa ctaagtcagg atgccttctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcggcatgaa caggacaatt tacttctcca gatggtttag tataagtaaa
    19021 gagatactga tctaacgttt gcttaatttt taatttcaac aattgatcag cttgtaccga
    19081 tttccattca cttagcaatg tcattacaac ttctggtact tgaatgacgg tatcttttcg
    19141 accttttgta aacttttttc tttgatattt atctaaagta tgtttaagac ggaccgtttg
    19201 tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc tcactttttc tatcaccaag
    19261 atacagggta atcataaaaa gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc
    19321 atcatttact gcttctatcc aattagtaag ttcttttgat gatagtgctt gcttaccttc
    19381 ttgtattctt ttttcttcta aagcattttt tttaggtgca gtgatacttt gaataatttt
    19441 agtagttcga ttaaattcga tataatttag tatttctgca atatcgaaca tggaacgaac
    19501 atatcctttt atgattttta tatttgcata ttctttcgat tttttggtta attca
//
LOCUS       DAYYHS010000056        19506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000056 DAYYHS010000000
VERSION     DAYYHS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.056"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHA6A_001908"
     CDS             127..3654
                     /locus_tag="ACHA6A_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8970078.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHA6A_001909"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHA6A_001909"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8970079.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHA6A_001910"
     CDS             7442..7783
                     /locus_tag="ACHA6A_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8970080.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHA6A_001911"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHA6A_001911"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8970081.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHA6A_001912"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHA6A_001912"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8970082.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHA6A_001913"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHA6A_001913"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8970083.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACHA6A_001914"
     CDS             12994..13731
                     /locus_tag="ACHA6A_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8970084.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACHA6A_001915"
     CDS             13745..14560
                     /locus_tag="ACHA6A_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8970085.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACHA6A_001916"
     CDS             14573..15220
                     /locus_tag="ACHA6A_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8970086.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACHA6A_001917"
     CDS             15236..15892
                     /locus_tag="ACHA6A_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8970087.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACHA6A_001918"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACHA6A_001918"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8970088.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACHA6A_001919"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACHA6A_001919"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8970089.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACHA6A_001920"
     CDS             18234..18752
                     /locus_tag="ACHA6A_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8970090.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHA6A_001921"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHA6A_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8970091.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6562 a         3348 c         4185 g         5411 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgt
//
LOCUS       DAYYHS010000057        19359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000057 DAYYHS010000000
VERSION     DAYYHS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.057"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..583)
                     /locus_tag="ACHA6A_001922"
     CDS             complement(<1..583)
                     /locus_tag="ACHA6A_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970092.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIF"
     gene            850..2550
                     /locus_tag="ACHA6A_001923"
     CDS             850..2550
                     /locus_tag="ACHA6A_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8970093.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2520..2738
                     /locus_tag="ACHA6A_001924"
     CDS             2520..2738
                     /locus_tag="ACHA6A_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8970094.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2758..3648
                     /locus_tag="ACHA6A_001925"
     CDS             2758..3648
                     /locus_tag="ACHA6A_001925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8970095.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3666..3950
                     /locus_tag="ACHA6A_001926"
     CDS             3666..3950
                     /locus_tag="ACHA6A_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970096.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4437..5051
                     /gene="gmk"
                     /locus_tag="ACHA6A_001927"
     CDS             4437..5051
                     /gene="gmk"
                     /locus_tag="ACHA6A_001927"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8970097.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            5056..5361
                     /gene="rpoZ"
                     /locus_tag="ACHA6A_001928"
     CDS             5056..5361
                     /gene="rpoZ"
                     /locus_tag="ACHA6A_001928"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8970098.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5614..8022
                     /gene="priA"
                     /locus_tag="ACHA6A_001929"
     CDS             5614..8022
                     /gene="priA"
                     /locus_tag="ACHA6A_001929"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8970099.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            8037..8528
                     /gene="def"
                     /locus_tag="ACHA6A_001930"
     CDS             8037..8528
                     /gene="def"
                     /locus_tag="ACHA6A_001930"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8970100.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8521..9459
                     /gene="fmt"
                     /locus_tag="ACHA6A_001931"
     CDS             8521..9459
                     /gene="fmt"
                     /locus_tag="ACHA6A_001931"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8970101.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9459..10817
                     /gene="rsmB"
                     /locus_tag="ACHA6A_001932"
     CDS             9459..10817
                     /gene="rsmB"
                     /locus_tag="ACHA6A_001932"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8970102.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10830..11570
                     /locus_tag="ACHA6A_001933"
     CDS             10830..11570
                     /locus_tag="ACHA6A_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8970103.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11567..13636
                     /gene="pknB"
                     /locus_tag="ACHA6A_001934"
     CDS             11567..13636
                     /gene="pknB"
                     /locus_tag="ACHA6A_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8970104.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13801..14700
                     /gene="rsgA"
                     /locus_tag="ACHA6A_001935"
     CDS             13801..14700
                     /gene="rsgA"
                     /locus_tag="ACHA6A_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8970105.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14713..15363
                     /gene="rpe"
                     /locus_tag="ACHA6A_001936"
     CDS             14713..15363
                     /gene="rpe"
                     /locus_tag="ACHA6A_001936"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8970106.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15364..16002
                     /locus_tag="ACHA6A_001937"
     CDS             15364..16002
                     /locus_tag="ACHA6A_001937"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8970107.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            16157..17011
                     /gene="mreC"
                     /locus_tag="ACHA6A_001938"
     CDS             16157..17011
                     /gene="mreC"
                     /locus_tag="ACHA6A_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8970108.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            17015..17524
                     /gene="mreD"
                     /locus_tag="ACHA6A_001939"
     CDS             17015..17524
                     /gene="mreD"
                     /locus_tag="ACHA6A_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8970109.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17779..19353
                     /locus_tag="ACHA6A_001940"
     CDS             17779..19353
                     /locus_tag="ACHA6A_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8970110.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6555 a         3543 c         4133 g         5128 t
ORIGIN      
        1 aaaaaatgat cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt
       61 tttcaacagg tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga
      121 tcgaacttaa tttactagca aaaatcctat aaaattcatc taaatagcga taataaatcc
      181 gattcagctg atcattaaga tcgaagtata caaaattatt ctgtagttta taaaaaaacg
      241 gcgaatgaag atgtgttttc atatgaccaa aatataataa ttctgaaatt tctaaaggtg
      301 tcaattcttt cagcatcgtt aaatcagtaa agtcgatcca cttcaattca tcgaatccac
      361 gcctgttacg agaagaggaa aagaaacgct gaatattttc tgtaccacgg attactttca
      421 gccctgtatg ggattcatat tctccagcag aagcagatgg atcaagcaag agcaagtttt
      481 gaggaaaatg cacgatactg tttgcaaagt cagtagcagt cagccctttt gttaaaacag
      541 cattacttgt ggtatcaata tgtacataaa ctgactcagt catcatgttc ctcctcttat
      601 ttcctcttaa tctcttttat tttacaacat ttgcattaaa aaagcttgtt tcttttattt
      661 taaaattcca ttacaaaatc acgttttcat tgaaaacact ctcattttca attaattttt
      721 gtgatttttg tattttttta tttaaaggag gttttatgaa ttgttttctt atggaatcta
      781 caaagtcgaa tacgagactc atgacctttt tatgacgtac tggtatactt gaagaaaaag
      841 gagagcgcga tggagataaa aaaagtttac ttttcacaga aaatgtcgct gtattcgcct
      901 atccagcaag caaaacaacc ccttccattt tttcaaagtc atcaagaaag tagagacgct
      961 ttttggcgtt ctgcaaaaaa gcaatgtgct tttaaagcag tatcgaaaaa atataccgtc
     1021 tattttacta ttgctaaaag tagaggagat gttattttta ataatctgtt gattgaaggt
     1081 gaaacttttg aatggaaaaa attttttcgg tatatggaag cttgcgctcg tttttttatt
     1141 aaagaaaact gttgtttttt atttcaatct cctctatcag aagaatggtt gcgtattttt
     1201 cataaaaacg gctatcaagg aaccacacaa ttaaataaaa agctggtcta tcatacagca
     1261 cttgttttag gtagaggagg agcgcacggt gcttatcaga ttggtgtgtg gcaggcgctg
     1321 aaggaacatg atatcaactt tgagattatc acaggtacct cggtaggtgc gttgaacggc
     1381 gcattgattt tacaaggaga tatgaaaaaa gctgtgaatt tatggaaaaa gctgtcgaca
     1441 agacaagtgc ttgcgttacc agaaatggct gcagttgaag accttcgtga acgcttctat
     1501 cgagaaagac gccaaatgac gaaaacagca ctaattgaag gcggggtttc ttctgctcca
     1561 ttggaaaaga tggtcaaaga caatttggat ttgtccttac tcaaacataa tcccaaaata
     1621 cgtttatata cggtgtctac taaactgcca gaactagcgg aagtagtgac tgatatccaa
     1681 cagacaaaaa cagaagaaat accagattgg atactcgcct ctgcttcatt ttatcccgct
     1741 atggcttatc gaaagatcgg aaaaaataaa tatgcagacg gtgggtatcg caacaaaata
     1801 cctattgata ttgcgattaa caaaggagca acagaggcat ttgtcgtaga tgtgcaaggg
     1861 ccaggcccag cgaaaaaaat tcgtatgcca gacattttta tccattggaa atgtcaaaca
     1921 ttgtggacac taggcagctt tctgcttttt gatagccaaa ggaaccaatt aaatttacaa
     1981 ttaggctatc tggaaatgaa aaaacgatta ggctattact atgggaactg gtatacattt
     2041 cattctgtta aacctgcaag aacatactgg cgaggatttt tgtcttactt aacaaaagaa
     2101 attcagttag aattatcttt tttcaaaagc ataaaatttt ggcaaaaact gcgtaattta
     2161 tataagaaca gggtcgtacc agagacatgc ggattggcga tgctagaact tttagcaaaa
     2221 aaacattttt tactgcccaa tgaaatctat caagtagaca tgatgattca aatgatctgc
     2281 caacaagata taaaaagtat gccggatgat ttattggcac aaattggtca attaagtact
     2341 gaagaatggc gaaggtatcg aaaataccag caaaaaatac aaaatgagcg cacgaaaact
     2401 gtttattttg attatctgct acaaggaaaa cgcaaggatc gtttgcagca tgaattgctt
     2461 aagcaaccag ttgacacatt actgatccta tatttatatt atctcaagga ggaacaacca
     2521 tggcacaaga attttcatat gaaatcgtag aagaaatcgc tatattgtca gaaaacaaca
     2581 aggggtggcg taaagaactg aacctagtca gctggaatgg tcgcccgcca aaattcgatc
     2641 ttcgagattg ggcgccagac catgaaaaaa tggggaaagg cctgacattg acgaacgaag
     2701 agtttgaaca actgcaaaaa gcaatagaaa atatgtaatg aggaaggacc ctgtcgaatg
     2761 aaaagtatga ctggttttgg caaagccatc cgcgaaacaa aagcgtatca gttagatatt
     2821 gaaataaaaa gtgtcaacca gcgttttcta gatgttcaaa tacgaagtcc taaaatcctg
     2881 aactttgttg aaaacgattt tcgtcagctt attaaacagc agttaagccg cggtcgaatc
     2941 gaagtatttg tgaatctagc ttatattggc aaaaacgaaa aacagatcgt catcaactgg
     3001 gaattgattg attcacttat cactcaattg acagacggaa ttcaacaacg atacggtagt
     3061 caaaatcaag tgtcaattag tcggatggcg gaacgcttcg tgctggacga aaacttcgta
     3121 acggtagaag aaaaaccgga agagagcatg gaagaattgg cacagttagc tctagataca
     3181 ttgaaaagcg cacttgattc tattgaggcg agtcgagaaa aagaaggtca agctttagct
     3241 ttagtgttga aaaagaatac tgaagaattc aaagaagtac taaaaaaact cgatagtttt
     3301 gtagaaatct atgaaaagga atttcaaata aggtatcaaa aaaagctgga ggaatttctt
     3361 ggagcgaaag tcgatgagca acggttgttg acggagctag ccatcttgtt ggaacgcgga
     3421 gatattcacg aggaactgga tcgtctggcg atccatgtac aaaaactaga tgaacttatc
     3481 gaagcacaat gtcctgtagg tcgggaatta gatttcctga tccaagaaat gaatcgggaa
     3541 atcaacacga tcggttccaa atcaaatgca atcgaaatta aaaatcaagt cgtccagtct
     3601 aagacgatct tagaaaaaat cagagaacaa gtacaaaata ttgaatgacg aaagaggtag
     3661 agataatgaa atattatgac gtaacgttcc acgaattaag tggaaaaact gtggttaaac
     3721 gggatatccc ttctgaaaaa aacggctttg acgtatggaa agatgcctgt gcagactata
     3781 atgagaacga actgtttatt ttgatcaacg acggtgctta cgtgacgatg aatcggaaat
     3841 ttattgttcg aattgacaca gaagaggtag aagatccgac agaaaaagca cgttctcgaa
     3901 aagatgagat catgggtgta gtgaatactt tgtccaatat gggcttttag caaggatgct
     3961 gaacaaagaa atacaataaa taaacagaat tggatttctt cgcttagctt ctttgctcta
     4021 ggaatacatt taaaggttgt gacaaaattc gtgtcacaat ctttttttat tttgaataag
     4081 tgatgttcaa aaaccatttt ctcggtatta agccaaattt atgcaaaaat gtgagaaact
     4141 attttcacaa aatttttctt catactcgaa actaatcggc ttcttcacga cctcttatac
     4201 acggcgttca aaagcttgca tctgcgataa taaccccaac caaacgaaaa acatgagaag
     4261 ctgtttatgt ttggctgttc ttattacttg atgcaggaca gcttttttac gacctcttgg
     4321 tacagggtag cttttttacc aatttctagc tataattaaa gataaccttg aaagttgcag
     4381 aaaaaaagtg caaaataaac gataagtaca aaaagactag ttgaaaggac gtcgtcatgt
     4441 cagagcgtgg attattaatc gtgttgtctg gtccatccgg tgtaggaaaa ggcacagtac
     4501 gaaaagcaat ttttgagagt gaggataatg attttcagta ttcgatctca atgactaccc
     4561 gtaaaatgcg tgaaggggag gtcgaaggcg ttgattattt cttccgaagc aaagaagagt
     4621 ttgaatcaat gatcgaagct ggagagatgc tggaatacgc agaatatgtc gggaattatt
     4681 atggaactcc tttgtcatat gtcaacaaga cattggatga aggaaaagat gttttcttgg
     4741 agattgaagt ccaaggagca aaacaagtca aagaaaaagt gcccgatggt gtattcatct
     4801 ttttgactcc gccagattta gcggaattaa aatcacggat cgtaggccga ggaacagatg
     4861 cagatgaagt aatcgaaaaa cgaatgaaag ttgcaaaaga agaaatcgaa atgatggctc
     4921 tttacgacta tgctgttgta aatgatgaag tgccgctagc agttgaacga atcaaaaaca
     4981 ttattgccag tgaacatttt cgagtagatc gcgtaatcgg taaatatatt aaaatgttaa
     5041 aggagatgta gtcttatgat gttaaaacct tcaattgact cactattaga tcgtgtaaat
     5101 tcaaaatatt ctctagtgat tttagcaagt aaacgcgcgc atgaattgga tgcaagtgca
     5161 cagcctactc tagattcttt tgattcagta aaaagcgtcg gtcaagcttt agaagaaatc
     5221 gatgcaggaa atgtagtcaa tgatccacat cctgaattaa aacgtgaacg tttgaagatg
     5281 gaagaagagg aacgtcaagc acaaaaagag cgtgaacaac acgaattgga atcacgtatc
     5341 cgtgaagagc aaaaaatgta ataaagaaaa atagagcgaa catctaaaaa gaaaagcaaa
     5401 cccagctgtt tccttttcaa acattcgcca cttattgtct ggtagaaaag gctgtgtctg
     5461 aactttttga cacagttctt ttttttctta tctgctgtta attttttgtt aggatttggc
     5521 agaagataag acaaatccat cgatctctag taaaataggc aaggaacttt ttgtcgcttg
     5581 ataagaaaag acaaaaaaag gaggaaacga accatgacat taacagctga agtcattgtg
     5641 gacgtaccta ctatgcagac cgatcagcca ttcacttatt tggttccctc tgaaatggaa
     5701 acagctttgc aagtcgggat gcgagtagaa gtcccttttg gtaatggcaa tcgtcatgtc
     5761 caaggatttg ttatggctat ccaaaaatca gaagaatcag ctaatccatc tttgaaggca
     5821 gttattcgtt tgctagacct tgctccggtg gtcaatgaag aattactttc attagctgat
     5881 tatatgaaaa aaattactta cgcattcaaa atcacttgtc tacaaacgat gcttccgagt
     5941 gtgatgaaag cagaatatga taaattgatc tatcctttgg cagacacacc agaagttcag
     6001 gtattgtttg atcagcgaga agtgatttcc tggaaggaag cagaagaaga aggcagcctt
     6061 tctcaattga ttcgctggcg ccaagagcaa ttagtagata tcaaatatga agtccataca
     6121 cgaaataaag taaaaacgat tcgtttggtc cgttctttgt taactgaaaa gcaaatcgaa
     6181 gaagaatggg caaaattacg gcaaaatgcc aagaaacaaa aagaacttct tttgtgttta
     6241 tctgaactga gccaagaaga accaattgct tatttcaaaa ataaaggaat cagtactgct
     6301 gtcttgaatc aaggaaaaga aaagggctgg ctggagttta tagaatcaga acgttatcgc
     6361 gacccttata aagaccgagt atttgaccaa acgacggcat tggaactgaa tgcagaacaa
     6421 aaaaatgctg tcgaacaaat catcacagca ggtcagcagc aaaaagatca ggtctttttg
     6481 ctggagggaa tcactggcag cgggaaaaca gaagtatatc ttcaggcaat cgctgatgtt
     6541 ttgtcagaaa ataaaacggc tattatgtta gtgcctgaaa tcgcattgac tccgcaaatg
     6601 gtcgagcgat tcaaaggacg cttcggagaa tcagttgcgg tcttacatag cggactttcc
     6661 caaggtgaaa aatatgatga atggcgcaag attgagcggg aagaagcgca agtagtagta
     6721 ggcgcacgat ctgctatttt tgccccgctg aaaaatatcg ggctgatcat tatcgatgaa
     6781 gaacatgaat caagttacaa acaagatgag acaccgcgtt atcatgcgag agatctagca
     6841 atttggcgca gtaaatatca tcattgccca atagttctag gaagcgcgac gccctcttta
     6901 gaatcaagag cacgagctca aaaaggcgtt tatcagttat tgcagttaaa ccaccgagcc
     6961 aaagcagctg cacagcttcc agctattgag atcgtagata tgcgtgaaga gtttcagaat
     7021 catcggacct ctactttttc tgctaatttg caggaaaaaa tacaaaatcg attagacaaa
     7081 aaagaacaaa ctgtcctact gctcaatcgt cgaggatatt cgtcttttgt gatgtgtagg
     7141 gactgtggat ttgttcttcc atgtcctaat tgcgatatct ctcttacact gcatatggat
     7201 acacgttcaa tgcgctgcca ttattgcgga catgaagaac ctattcccaa tcgctgtcct
     7261 aattgtggcg gcaataaaat ccgctactat ggaacaggta ctcaaaaagt agaagaggaa
     7321 ctccgagaac tttatccgca agctaggatt ttgcgtatgg atgtagatac cactcgaaga
     7381 aaaggggcac acgaacagat tttacaaaaa ttcggtgcaa aagaagcaga tatattatta
     7441 gggacacaaa tgattgctaa aggtttggac tttccagaag tgacattagt tggggtgttg
     7501 aatgctgata cctccctgaa tttaccggat tttcgttcta gtgagcatac attccaattg
     7561 cttacccaag tttcaggacg agcagggcga gcggaaaaag caggcgaagt cgtgatccaa
     7621 acattcaatc ctcaacatta cgccatagaa ttggctaaaa aacaaaatta tgagcagttt
     7681 tatcagcagg aaatgcatgt acggcatcgt ggaggctatc ctccttatta ctttactgtg
     7741 aaaataaccg ccagccatcc tgaagaacaa gtagcggcaa agaaaatctt tcagattgcc
     7801 aatcagctaa aagaagtgtt aagccctcaa agcttgctat tagggccaac gcccagcatg
     7861 attttgagag tgaaaaaccg atattattat caactaatca tcaaatataa acatgaaccg
     7921 aatcttcctc atgttttgga tgaaattttg aatggaagcc agaaagaaca gcgacaaggt
     7981 ctttttgtgg caattgacaa cgaaccatta aactttattt aagaaagggc caaattatgc
     8041 gctatcctat attgatccac cctaatgata aattaaaaag aactgctcag ccaatcgatg
     8101 taatcacgga cgagacaatc gctttattag acaatttata tgagacaatg attgcgaatg
     8161 acggaatagg gatcgctgct cctcaagtag gacaaaacaa gagaattgct gtgatcgaag
     8221 tagatgaagg cgagaaattt gaactgatta acccagaaat catcgaagca aaaggtgaaa
     8281 gccttgacgt agaaggttgt ctcagtattc cgcatgtcta tggaacggtc aagcgagcag
     8341 atgaagtgac cgtgcgttat tatgatcgag acggagaaga gatagaagtg acggcttttg
     8401 gctatttagc ccgtgccttc cagcatgaaa tcgatcactt agacggtatc ttgttcattg
     8461 agaaaatgat acagcagatc ccggaagaag aacttgaaga atatatggag gaacatcttg
     8521 atgactaagc tagtatttat gggcacacct gctttttctg tgccgattct tgaagggctg
     8581 atagaatcag gttacgagat ccaggcagtg gtaacacagc ccgaccgtcc agttgggcgg
     8641 aaacgagtaa ttacgccgac accggtaaaa aaagcggctc tgaagcacgg tatccgtgtg
     8701 ctgcagccag agaaaatctc cggttcgcca gaaatggaag aaatcattga attggtgcct
     8761 gatgtgatcg tcactgcggc atttggccaa ttcttgcctg aaaagttatt gcaagtacca
     8821 aaattagggg caatcaatgt ccatgcttct ttacttccta aatatcgggg aggtgcgcct
     8881 gttcattatg ccatcatgaa tggggagaaa gaaactggtg taacgatcat ggaaatgatc
     8941 aaaaaaatgg atgcaggcgg aatctatgcg caagaaagca taccaatcac taaacaagat
     9001 gatgtaggga cgatgtttga aaagcttagc cttttaggaa gaaagctatt gctagaaaca
     9061 ttgcccaata ttttggatgg acaaaaacca gtacctcaag acgagtcaga agcaactttt
     9121 tcgccaaaca tcacacggga acaagaagca atcgattggc ataaaactgc agaagaaatt
     9181 gacaaccaag tacgcggaat gcgtccttgg ccaattgcat tcacgaccta tgagggcata
     9241 cgctggaaat tattatcggt tgaagtattg gcagaaacta cagacaaagc acctggtacg
     9301 atcatccgga aagacaaaaa aaatctatgg attgcttgcg gtaaacagac cgtcttagcc
     9361 atcaatgaat tacagccagc tggtaaagga aaacaggccg tacatgattt ccttaacgga
     9421 agcggtcagc aagttcagat tggtcagcag gtgaaataat gaaaaaaatc cctaaaaaaa
     9481 tgaaaaaatc agtacgatat atggcattac tgacattaga gcgagtgaac aagggtggcg
     9541 cctactcgaa tcttttgttg aatgagatga tcaaacaaag cgagctaaat caaaaagatg
     9601 tgggtttatt tacagaattg atctatggta cgattagcca tcagcgacta ttggaatttt
     9661 acgttgcacc attgatcgct aaagcaaaaa aagtggatga ctgggtcaag atgcttcttt
     9721 atctttctgt ataccaatta gaatttttag ataaagtccc tgcacatgct attttaaacg
     9781 aggcggtcga aatcgcaaaa gtaaaaggaa atcctgggac agggaaattt gtaaatggtg
     9841 tcttgaggaa ttatcagcgt caaggagcac cagatctgca aggcattgct gatcctatcg
     9901 aaagactttc tgtcaaaatc agcctgcctc tatggatgac gaaacgactg gtggatcaaa
     9961 tcggatacga agaaactgaa aagctcggtt tatcgctata tcagcctagc catgctagtg
    10021 cccgagtaga tacacgccga ctttccagag atcaggccat cactgttttg caagaagaag
    10081 agatcacagc tgaaaaaagc caagtcagtc catacggtat agtagcgaaa aaagggcatt
    10141 tagcttctag ctcattgttc cacgatggcg tcatgacgat ccaagatgaa agttcgatgc
    10201 ttgtagcgcc gtctatgcaa atcgagcctg aacatcatgt cttagatgcc tgtgcagcac
    10261 ccggaggtaa gacgacgcat attgcttctt ttttagatcc gtcatgcggt ggagaagtgg
    10321 tcgctttaga tgtacacgaa cacaaagtca aactcattga agataatgca gcacgtctag
    10381 gtgtctcttc tgctgtctac gctcaaaaga tggatgctag agaagtcagt aaacagttcg
    10441 aagcagagca gtttgatcgg attttagtag acgcgccttg ttcaggtctt gggctgcttc
    10501 ggcgaaaacc agatattcgt tataaaaaac agccggatga attagcgaat ttaccaaaaa
    10561 tccaattgga aattttagaa agctgcgcac caactttaaa gtcttccggt attctgactt
    10621 atagcacttg tacgatctta aaagaagaaa accaagacgt tgtttctgct ttcttggaaa
    10681 gacaccctga ttttgagagg attgatgttt tgaccaatcc tatgctacaa tcatcaatcg
    10741 aagagaaaat gttgactttg tatccgcatc agttttacac agatggattt ttcatttgtt
    10801 gtttgcgcaa aaagtaatga ggtgaaaaaa tgcagattga atatcaatca gatgtaggaa
    10861 gacgacgtaa cacaaatcaa gactatgcca gtgtatttac cagccgttca ggtctcaagc
    10921 ttgctgtatt agcagacggg atgggtggtc atcgtgccgg agacattgcc agtcagatgg
    10981 cggtaacgaa tctaggcgaa gcttgggaaa aacaagaagc tgttgacgat gaaaaaatcg
    11041 cccaatggtt cattcaatcg attcaagaag aaaatatgct gatttatcag cgtggacaag
    11101 aacagccgga atataacggt atgggaacaa caatcgtagc ggccgctctg tcagatgact
    11161 ggttcacgat tgccaatgtt ggggatagca gagcttattt gattcgtgaa gataaaatca
    11221 tgcaattgac ggaggatcat tctttagtca atgaactagt caaatcagga gaaatcagcg
    11281 aagaaatggc agtgaaccat ccaaggaaaa atattttaac acgttctgtc ggcatgcctg
    11341 gaacagtcga agtagatgtt tctactcatc tttggcaact aaaagatcga ctgctgttat
    11401 gttcagatgg cttgacgaac atgctgtctg aagaagaaat aggaacgatc atcaatcaag
    11461 aaggcccatt gtctcataaa gtaactgagc tgattgatcg tgccaatgaa gcaggcggag
    11521 cggataatat caccgttctt cttatcgaat ataaggagga cagtgtatga tcgagttggg
    11581 caaaaaatta aatggccgtt atcaaatcac tggaaatatc ggtagcggtg gtatggcaaa
    11641 tgtatttttg gcgcacgatt taatcttgga tcgtgatgta gcagttaaag tcttacgatt
    11701 tgactttcaa aatgaccaga ctgcgattcg acgatttcag agagaagctt tagcagcaac
    11761 agaactcgtt catccaaaca ttgttagtgt ttacgatgtt ggtgaagaag acaacatgca
    11821 gtatctggtg atggaatacg tcaaaggaat ggacttgaaa cgatatatcc aaacacatta
    11881 tcctgttcct tatgaaacag cagtaaatat tatgcagcag atcctttctg ctatttcttt
    11941 agctcataac catcaaatca tccaccgaga tttgaaaccc caaaacgtac tgatcgataa
    12001 tgaaggtgtt gtaaaaatca cggatttcgg gattgctatc gctttatctg aaacatcgat
    12061 cacgcagacg aatacgatgc taggatctgt tcattatctg tcccctgagc aggccagagg
    12121 aagtatggca acgaagcagt cagatattta tgctttaggg atcatccttt atgagatgtt
    12181 gacgggtagc gtaccgtttg atggtgaatc tgctgtaaca attgctttaa aacattttca
    12241 agatgatctt ccatctataa aagcacttga tccaaatgtg ccacaagcac tggaaaacgt
    12301 gattttacgg gcaacggcta aagaacctgc agaccgttat aagtcagccg aggaaatgtc
    12361 tgatgatctt tctacagtat tatcaccagc tcgtgcaaac gaagaaaaat ggcagcctca
    12421 tgtgatggat aatgaaacaa aagttatcac accccttacg gaagatgcac caatgcctga
    12481 ttcattcaaa tctatgcctc tgccaaaaga caagccagaa aacgaaacct taccagagga
    12541 ggaagaagtt ctgcctgatc ctaaaaagaa aggacagaaa aaatggtggc tgatcctatt
    12601 agctattctt gctgttttag gaatcggaac agctgtattc tttgcttctg gcgggcgagg
    12661 agaagttgtt gttcctgatg tttctgatct ttctgaagct tctgctagag aaacactcgc
    12721 aaaagcgaaa ttagaagtag caacaaaaac agaagaaatt gcagatgaca aaatcgaaga
    12781 aggtaatgta gtgaagacag acccggcagc gggaacgaca gtcaagcaaa aacgcgaagt
    12841 cactctgtat attagtagcg gcacgaaaaa aatcaaatta gatgattaca gtggtaaaag
    12901 ttatgaagat gccgaagcaa gtttgaaaga attagggttt tctgctgact tgattcaaaa
    12961 agaagaggaa ttcagttcag aggtagaaag cggaatgatc atttcgcaat ctccatcaga
    13021 agatacagaa gtcgatccta aaacagacac gattactttc acagtcagca aaggaccaga
    13081 aacagtgact ttgagtagtc atgtaggaga aaactacgaa actgttcgta attggcttgt
    13141 tacacaagga ttcaatatca ataatattag tgctacttat gactatagcg acactgtacc
    13201 atcaggttcg atcatcagtc aggatccagc ctcgggtgaa gttgtagcag aagaaactta
    13261 catcaatttt gtcgtaagcc aaggaacaga gcctaaattg aatgatatct ccggctatac
    13321 aaaaagcgaa gcacaaagtt atttagctag tgtcggtgca gaatatatcg ggcatgagac
    13381 ttccgaatat tcaaacacgg tggataaaga taaagtgatt cgtacagtac caggggcagg
    13441 aacgacaatt accaaaggaa tggttgtgaa cgtaatttac tctaaaggac cagatccagc
    13501 gtcttctagt tctgaaaaag actcttcaga cagttctagc agctcaagta gctccagtag
    13561 cacaagcaac tcttcaaaag aagaatcttc cagctccaca agttcatcag ctagcacctc
    13621 agaaagttca aaataagtgc tgaataaaat atatcagaag ggaatgcgac attgatttgt
    13681 tgcattctct tttttgtgtt tctatctctt ttgatcaatt ttcaagccat atactttgag
    13741 aagtttgcat ggaataacac ctactttcct cttcgtatgg taaaataaac aaaaacggag
    13801 gtggcactta tcaaaggaca aatcagaaaa gcaatcagcg gtttttacta tgtatacgca
    13861 aacggagaga cctatcaaac aagaggaaga ggaaatttcc ggaatcggaa aatcacgccg
    13921 cttgtcggag atcaggtgat ctttgagagt gaaaatcaaa cagacggcta tcttctggaa
    13981 atcttgccga gaaaaaatca attagttcgc ccaccagtca caaatgttga tcttggaatc
    14041 attgtcacaa gtatcgtaga gcctgcattt tcttataatc tacttgaccg ctttctagtt
    14101 accttggaat acgagtcgat cgaaccgatt atttttttga caaagaccga cttgtcagac
    14161 gacccagaaa aaatcgaaga gatcaagcaa acctatacta aaataggcta tccggtgatt
    14221 gtacccaatc atacgggtga tacgacagaa ctgatccgtt acttcccaga acggctatcg
    14281 gtctttatgg gtcagtctgg agcaggaaaa tcgacgctac tgaatcaaat atcaccggaa
    14341 ctgaatttag caacaggaga gatttctgat gcacttggaa gaggacggca tacaacacgt
    14401 catgtggaac tgattcctat ctatggcggg ttagtagcag atacaccagg atttagctcg
    14461 attgactttt tgacaattga agcaggagaa ctgccgagac aatttcctga atttgtagaa
    14521 gcttctgtcc attgcaaatt tagagagtgc atgcatgcta aagaacctgg ctgcgaagta
    14581 aaacatagag tagaaacagg tggtatcgca cagacacgtt acgacaatta tttgcaattc
    14641 ttacaagaaa tcgaaaaaag aaaaccaatg tatgcaaaaa aagaaggtaa acgaaaatag
    14701 gaggaaacaa gaatgaagat agcaccatcc attttgagtg cagattttgc caatttacaa
    14761 agagacattg agttagtgga aaaaggaggc gcagattaca tccacgtaga tgtaatggat
    14821 ggccgatttg tcccaaatat cacattaggg ccaaatattg ttcaggcaat ccgtcctgtt
    14881 actaaattac cgttagacgt ccatctgatg atcgttgatc cagaaaacta cattccagca
    14941 tttgcaaaag ctggagcaga tatcattact gtccacgtcg aagcaactcc tcatattcat
    15001 cgggcacttc aaatgacaaa aagcttagga gttaaagctg gagtagttat caatccagga
    15061 acaccagtct ccatgatcaa acatgttttg cctttggctg atcaagtact agtcatgact
    15121 gtcaatcctg ggttcggcgg acaaagcttt attgaagaaa cagtagaaaa aattacggaa
    15181 ctcgctgatc tacgtagaga gaacaattgg acttatgcaa tcgaagtaga tggcggaatc
    15241 gttccagaaa cagcaaaaat ctgtaaagat gcaggggcgg atgtcttcgt tgcaggatca
    15301 tatatctatg gagccgaaaa tccagtcgga caaatcgaac gattaaaaga ggcattagac
    15361 taaatgaaaa ttttattagc tgctggaggt aatccgcaaa actggccgca gtctctgctg
    15421 tccaaatacg atgcctatgt cgggatagac cgtggtgcgc tgtacctgct tcaagcaggc
    15481 tacccagtag atctggcagt gggtgatttt gattcattgt cgaacgaaga gcagaaggaa
    15541 gtatttgcaa aagccaaaga gtatacccgt tcccctgcag aaaaagacga tacagataca
    15601 cagttaggta tcttaaaaac atttgaacgt tacccgcaag cggaagtcac tttgattgga
    15661 gctacgggcg gaagattgga tcatctttta gccaatctct ggttaggtat ggaacctcgc
    15721 ttcaaaccct ttgcccatca gcttattatc agagattgtc aaaattattt gtcttattat
    15781 ttgcctggag agcatatagt aagacagata ccagatatga agtatttagc ttattgctgc
    15841 ttgactcccg tctcgaatct tacattaaaa ggcagcaagt atgtattgga aaataaagaa
    15901 gtagaatatc cgacatctta tgcaagtaac gaatttcttt cagataccgc agaattttct
    15961 ttttcagatg gtatgattgc agttattcaa agcaaagatt gatccataaa caaataaaaa
    16021 aatttgtctt tcaggctaaa aatgacagaa tctattattt gctaaaaaaa agcttaagag
    16081 gagcaactta ccttttgttt cacggcattt agtgataaaa ttgaacacaa gagtctaatt
    16141 aattcgaggt gtctttgtga agaagttcaa tcctaataaa aatattatta ttaccttggt
    16201 tattgttatc atcgtggtga cgatactaag tgtgaccgct gcgcagcgag cgaatgaagg
    16261 aaagcccaat ttcttccaat ccatcgttaa tgacagtgtt gcaatggttg ataaaacaat
    16321 ctctgcccca gtacggtggg tacgaaatgg agtagattct gtccataatc ttttttcaac
    16381 ttatgccgaa aatgagcggc taaaagaaaa aatcgatagc tatgatgacg tttcacagca
    16441 aaacaagaat tacgagagag aaatcgaagc gttgaaaaaa gaacttgagt tgaatcagac
    16501 gctgactagt tacgaaaaag taacggcaaa tgtcattact cgttcgcctg acagttggca
    16561 ggacatgctg atcgtagaca agggttctaa agacggtatc gaagcaaata tggcagtcat
    16621 gtcgcaaaaa ggattgattg gccgagtgat cgaggtcaat acggcttcgt ctaaaatcga
    16681 attactgtca tcctctaatg aaagctccaa tcattttcca gtacgggtat cttcggctaa
    16741 tggcgaagcg tttggtttgc ttaaaaacta tgatgaaaag ctccatgcct tagtggtgac
    16801 ccaattaact ggtgatacgg atatcaaaga aggggatgtt gtccagacat ccggtcttgg
    16861 agggaattct ccagctaact tgccgatcgg tacggttatt aaaacgaaac cagatagtta
    16921 tgggctggat cgggaagttt atgtgaaacc ttatgcagaa atgtatgacg tgtcagttgt
    16981 gacgattgtc caacggctag cggaggatta gtccatgttg aagaaagaaa caatgaagta
    17041 ctatctgcca atcgttttgt tctttttgat gttgatagat ggtcatttaa caagaatgct
    17101 aggggagtgg tcgaaaggca cctatatgtc aaatgcccac tttctgatat tggcattatt
    17161 atgttgcagt atggcgtttg aaaaacgtta tttactgatt accacgattg ttctcggggc
    17221 tatctatgac gcttactata ttggcgttat cggtatctat gcagtagctc tccctttaat
    17281 tgtatggttg atgtatgtaa tgaaagacgt tatccatgtc aacatcttta ctgaattttt
    17341 cagtatgatc atctttgtca cgggttatga attgtttacg atggtggtcc agttgatttt
    17401 taaattagca gtagtaaata acacgtattt tattacaagg tttttaggac ctacactgct
    17461 gttgaacatg attatatttg tattattcat ttttcccttt aagaaattat tcagtgatga
    17521 ataatttctt tttttatttt taggtcgaaa gcgaaaaaga atcgttgtta tcttaagaaa
    17581 tgctagactt aacgcggctt tttcatgatc attttctgaa acgaacagtt ttgtcatttt
    17641 taaaagttgt tttgaaacat ttttgtaaca taactattat atcgttgaca cactgctatg
    17701 gtacaatttt tatatcgtta agaaagactt aaatatattt agatatttat taaattaagc
    17761 cagtcggagg aatgaagagt gaaaaagagt ttaatatcag cagtaatggt atgttcaatg
    17821 accttgactg ccgtagcatc accgatcgct gccgcggcgg acgattttga ttctcagata
    17881 caacagcaag atcaaaagat tgcagacttg aaaaatcaac aagcagatgc acaatcacaa
    17941 atcgatgcct tagaaagcca agtttctgaa atcaacacac aagcacaaga cctattagca
    18001 aaacaagata cattgcgtca agaatctgca caattagtaa aagacattgc agatttacaa
    18061 gaacgtattg aaaaacgtga agatacaatc caaaaacaag cacgcgaagc acaagtaagc
    18121 aacacaagct caaactacat cgatgctgta ttgaatgctg attcattagc agatgctatt
    18181 ggacgcgttc aagcaatgac aacgatggta aaagcaaaca acgacttgat ggaacaacaa
    18241 aaacaagata aaaaagctgt agaagacaaa aaagctgaaa acgatgctaa actaaaagaa
    18301 ttagctgaaa accaagctgc tttggaatct caaaaaggcg atcttttatc taaacaagct
    18361 gatttgaacg tactgaaaac ttcattagct gctgaacaag caactgctga agacaagaaa
    18421 gctgacttga accgtcaaaa agcagaagct gaagcagagc aagctcgtat tcgtgaacaa
    18481 caacgtctag cagaacaagc tcgtcaacaa gctgcacaag aaaaagctga aaaagaagca
    18541 cgcgaacaag cagaggctga agcacaagct actcaagctt catctactgc acaaagctca
    18601 gcaacagaag aatcatcagc tacacaatct tcaatgactg aagaatcatc atcagctaca
    18661 caatcttcag caacagaaga atcaacaaca cctgaatctt caacagaaga atcaacagcg
    18721 cctgaatctt cagcaacaga agaatcaaca acagcgcctg aatcttcagc aacagaagaa
    18781 tcaacaacag tgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca
    18841 acaacagaag aatcaacaac acctgcgcct acaacaccat caacagatca aagtgttgat
    18901 acaggaaacg gcacaggaag tagtactccg gctccaacgc caacaccaac acctgaacaa
    18961 ccaaaacctg taacacctgc tccagctcct tctggaagtg tgaatggtgc agcaatcgta
    19021 gcagaagctt acaaatatat tggtactcca tatgtttggg gcggtaaaga tccaagtggc
    19081 tttgactgct cagggttcac acgctatgtt tacatgcaag taactggtcg tgacatcggt
    19141 ggttggacag ttcctcaaga atcagctgga acaaaaattt cagtttctca agcaaaagct
    19201 ggagacttgt tattctgggg gtcacaaggc ggaacttacc acgtagcgat tgctttaggc
    19261 ggaggacaat atatccatgc tcctcaacca ggcgaaagcg taaaagttgg ttcagttcaa
    19321 tggtttgccc ctgactttgc tgtcagcatg taagaatga
//
LOCUS       DAYYHS010000058        19075 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000058 DAYYHS010000000
VERSION     DAYYHS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.058"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..1497
                     /locus_tag="ACHA6A_001941"
     CDS             70..1497
                     /locus_tag="ACHA6A_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970111.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1589..1954)
                     /locus_tag="ACHA6A_001942"
     CDS             complement(1589..1954)
                     /locus_tag="ACHA6A_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8970112.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1951..2316)
                     /locus_tag="ACHA6A_001943"
     CDS             complement(1951..2316)
                     /locus_tag="ACHA6A_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8970113.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2508..2756)
                     /locus_tag="ACHA6A_001944"
     CDS             complement(2508..2756)
                     /locus_tag="ACHA6A_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970114.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2755..2883
                     /locus_tag="ACHA6A_001945"
     CDS             2755..2883
                     /locus_tag="ACHA6A_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8970115.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2940..4634
                     /locus_tag="ACHA6A_001946"
     CDS             2940..4634
                     /locus_tag="ACHA6A_001946"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8970116.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4734..4907
                     /locus_tag="ACHA6A_001947"
     CDS             4734..4907
                     /locus_tag="ACHA6A_001947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970117.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5285..6418
                     /locus_tag="ACHA6A_001948"
     CDS             5285..6418
                     /locus_tag="ACHA6A_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8970118.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6449..6769
                     /locus_tag="ACHA6A_001949"
     CDS             6449..6769
                     /locus_tag="ACHA6A_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8970119.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6798..7262)
                     /locus_tag="ACHA6A_001950"
     CDS             complement(6798..7262)
                     /locus_tag="ACHA6A_001950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8970120.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7394..8647)
                     /locus_tag="ACHA6A_001951"
     CDS             complement(7394..8647)
                     /locus_tag="ACHA6A_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8970121.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     SPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8676..8993)
                     /locus_tag="ACHA6A_001952"
     CDS             complement(8676..8993)
                     /locus_tag="ACHA6A_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8970122.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9019..9339)
                     /locus_tag="ACHA6A_001953"
     CDS             complement(9019..9339)
                     /locus_tag="ACHA6A_001953"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8970123.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9803..11218)
                     /locus_tag="ACHA6A_001954"
     CDS             complement(9803..11218)
                     /locus_tag="ACHA6A_001954"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8970124.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11235..12551)
                     /locus_tag="ACHA6A_001955"
     CDS             complement(11235..12551)
                     /locus_tag="ACHA6A_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8970125.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12701..13399)
                     /locus_tag="ACHA6A_001956"
     CDS             complement(12701..13399)
                     /locus_tag="ACHA6A_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8970126.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13688..14677)
                     /locus_tag="ACHA6A_001957"
     CDS             complement(13688..14677)
                     /locus_tag="ACHA6A_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8970127.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14757..15590)
                     /gene="msrA"
                     /locus_tag="ACHA6A_001958"
     CDS             complement(14757..15590)
                     /gene="msrA"
                     /locus_tag="ACHA6A_001958"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8970128.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15765..17399)
                     /locus_tag="ACHA6A_001959"
     CDS             complement(15765..17399)
                     /locus_tag="ACHA6A_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8970129.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17386..19074)
                     /locus_tag="ACHA6A_001960"
     CDS             complement(17386..19074)
                     /locus_tag="ACHA6A_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970130.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6064 a         3740 c         3176 g         6095 t
ORIGIN      
        1 atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat acaaggcgga
       61 ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct gtttttatgt
      121 gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct cctgcatgtt
      181 tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga agaacagatc
      241 gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca tatacctcga
      301 tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg tctgctttat
      361 ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc
      421 aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg gatcgaaatc
      481 gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg
      541 tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga acgtgccgat
      601 cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga aaataaagaa
      661 atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata
      721 gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt cgaatcagca
      781 tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt tattttgaca
      841 ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga agactttcaa
      901 cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt tgctaccttt
      961 gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc gctgattcat
     1021 ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt ttatctttta
     1081 aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag ctggtccagc
     1141 cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt gatcgaattg
     1201 agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac aaattcggat
     1261 gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga ttttcaagtt
     1321 gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca gtcctcaaac
     1381 cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga aaatattatt
     1441 cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa caaataaggg
     1501 gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt tctgtaacaa
     1561 tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctacg ggtccgatct
     1621 ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct agttggaaaa
     1681 tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca agaatttcca
     1741 aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg ctgactcggt
     1801 cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt gatagacaaa
     1861 caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat
     1921 atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc tttgtttttc
     1981 tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga taataataag
     2041 tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga tatcagcaac
     2101 gactagtact tccaagctta acaaaatata acttgctagc agttttcttg tatctgcatt
     2161 acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa gtttgaatag
     2221 aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa tagcaatgat
     2281 ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact ttcttttaat
     2341 ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta aaagttgttc
     2401 taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct ccctccatcc
     2461 ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta gtccgtcatc
     2521 agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct aaaaatagaa
     2581 ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac aggaaaatca
     2641 gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat ttggcattct
     2701 aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt tttcatgagt
     2761 aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc tgcatatcta
     2821 ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt cgaatcaatc
     2881 tagcatccta tttagttgaa tcactacgga catacttgga agacattgga ggaatcacaa
     2941 tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca gcggctgttt
     3001 atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca ttggatgatc
     3061 acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc
     3121 atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt aattaccgta
     3181 cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag ccagggatga
     3241 ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac aaagcacgtt
     3301 tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat aataaagacc
     3361 gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa ctagacaata
     3421 tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac ttctggttta
     3481 tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg cgtcgctata
     3541 tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac
     3601 gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa gaacaaggat
     3661 gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca ccgatgcaag
     3721 atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa gaagaatacg
     3781 ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca gattcagcta
     3841 ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca gcttctagtc
     3901 tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa ttctttgctg
     3961 atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat ttgttgttga
     4021 atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca tttatttcaa
     4081 ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc
     4141 aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat ttcctttatc
     4201 caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga aaagaaatgc
     4261 tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat attcgtgaaa
     4321 aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg ccttatgctt
     4381 ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct aaacattcga
     4441 ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc tttactgaac
     4501 aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt cctatgtccc
     4561 gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt gccgcaaaaa
     4621 aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa
     4681 ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg cagatgcaag
     4741 cttttgaaca ccgtttacca agaggttatg aaaaagctgc cctgcatcaa gtaataagag
     4801 cagccaagct aaaacagctt cttatgtttt tgacttggtt agacttatta ccgcagatgc
     4861 aagcttttga acaccgttta ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa
     4921 gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa atttgactta ataccgagaa
     4981 actgatccct tctgtcataa cctcttttta tttatgtctg tttctttttg aaaacaacta
     5041 ttttttcaac gtttactctc ttgccaatcc aacatcatct aacggataca actatttcaa
     5101 tgattaccat tacactaaaa aatcggaaac acacctctcc gaacacaaat acataagact
     5161 atagaatgtt tcatttaaga aaaaaagcgt ttcattatca aaaaggtaag atctccttta
     5221 aaatgaaatt atctgtagta ctaataatgc agacaatttt atgaatctga aaggagcatc
     5281 tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga
     5341 tccaaaaata ttagaaccaa cagatgcaat catccgtatc acagcaacag ccatttgcgg
     5401 ctccgatctg catctttacc atcatggtga agcagttttg gaaaaagggt atgttgtagg
     5461 acacgaacca atgggaatcg ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg
     5521 gcaacgtgtg gttatccctt ttaatattag ttgtggtcat tgtcatttct gcctgaatca
     5581 catggaaagc caatgtgatg aatcaaatcc agatcagtta tatggcgggc ttttcggatt
     5641 tggcaagttg aacggtaatc attggggtgg tcaagcggaa tatctaagag ttccttatgc
     5701 agactctact tcatttgttg taccagataa tgatcttcct gatgaaaaag ttttatttct
     5761 atcagatatt ttgcccacag cttattggag cgttgaaaac gcaggagtaa aaaaaggcga
     5821 tacagttgtg attctaggtt ctggtccagt cggtctattc gctcaaaaat tcgctgttat
     5881 ggctggagca gagcgcgtaa tcgcagtaga tccagtacag caccgtctag ataaagcggc
     5941 taattacaat ggggttgaaa cttatttctt agaagacacg gcacaagctg gaacagaatt
     6001 gtatgaactg gctaaaggtg gcgcagatgt cattatcgat tgtgtaggaa tggacggatt
     6061 agagcctgtc aaagaaaaag cgaaaaattt agtaagctta caaagcggca caatctctcc
     6121 attacagatg gcatcgcaag ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta
     6181 tatgactcca gcttcttctt acccgctgca agaatttttt atgcgggata tcgctgtcaa
     6241 gcatggacag gcaccaatta tccatctaat gccaaaaatt tacgcaatga ttgcagaagg
     6301 actattcgat cctacacaaa tcattactca ttcgctgcca ctatcagaag cagcacaagc
     6361 atatgagatc tttgacaaaa aagaagataa aaatataaaa gttgttttga aaccttgaat
     6421 tcagtaaaaa atacaggtga ttttctttat gcccaaaatc aaacgaaata cccatgagat
     6481 cgaaatagga aaagaagaag atatcgaaat acgaggacgt cgcttccgct tctatttcca
     6541 aaatcgttac acattgatct cactggctgt cgatttatta accgggattt tctatatttt
     6601 tggaagcctt gcttccttaa ctaaaatccc cgatcgatat ggcacctatt tttatttggc
     6661 tggtgctatt ttcttgacga ttcgtcctat tctcagaatc gtaaggaaca tttttattta
     6721 cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa gaggcatgag aatttttcaa
     6781 atctcatgcc tcttttttca ccctatcgta tcactttgat acagacgagc ggtcgtccca
     6841 ctttcttcct tgaccattgt caaaaatgtc cactcgtatc gttcataaaa cgtggtatga
     6901 tctgtaatta agtaaagttt ttcgtaccca agttgatgca tttcttttct gatttccgct
     6961 aatatccgct tggctatccc ctgcttacgc ttttccggtt caacaaacag ggcacaaaga
     7021 tttggtgtca gatcagggcg gttatggaag tcgttttcaa tgacgcccgc tcctccgacg
     7081 atggtttctt tttcatctaa gaccacatac cattgaggaa tactttcctt attagcgatt
     7141 ccagtacgaa tactttcctc atacacaaca gcaggaactc cccattttga tgaaaaccaa
     7201 ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt tgatccagtt atatgtaacc
     7261 aatgtttaaa cctccgacta aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa
     7321 attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc
     7381 gttttttgat taatcaagca tctaaagctt cttttcttct ttcttggatc gtagctacct
     7441 tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt gacagcggct agtacaccac
     7501 ctgcaatact ttttgttgcc aatacaccgc caataattgg tggagttacc catggaggca
     7561 taacggtagc tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga
     7621 ccattggtgt caaaataaat ggaatgaaca tgatcgggtt caacacgata ggtaacccaa
     7681 acatcgttgg ttcattaata ttgaagacac ctggcgcaat cgacaagttt gttacaacca
     7741 tatatggttt gtttttacgt cctactaaat aaatagctaa cagtaaacct aaagttgcac
     7801 ctgttcctcc taagttgaca aagctgtcaa agaaaggttt gttcacgata aacggtgcgc
     7861 tatgtccagc tgcgattgca ttaacgtttg cttcaatggc tggcgcattg attgtttgca
     7921 tcaaaggatc gaccatattc gctccgtgta aaccaaagaa ccataagaat ggtgtgataa
     7981 atgcaagcag taaggcagat ggataagagt ttgcaagtcc cataaatggt tcttggacca
     8041 ctgtatagaa agaacctacg atatcatcaa caccgaatgc agcaaaaatt gcagcaacta
     8101 aagcaaacgc agagatagtg atcattgctg gtagtaatga agcaaatgct tttgcaactg
     8161 ccggtgacac accatcaggc attttgatga tcaatttttc atttccgctt aaacgtacaa
     8221 agatttctgt tgaaataatc ccaacaataa tagcaataaa caaaccgttt gagctcatac
     8281 ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt
     8341 cttttcggta gccgttagct aaatgataag caagcaagaa agcaatcaag acagaaagaa
     8401 tggcaaatgt accgttccaa gcagcagccc cgaatccttt ccatgattca ccaccaaaaa
     8461 ttgagttcat caattcttgg aacccgggaa tcggtaagtt attgatcatt actgctaatg
     8521 ctcctaagat cattaacggc atactcacca taaatgagtc ccgaatcgca actaaatgcc
     8581 gttgtgcacc aatttttgat gctacaggta taaaatgttt ctccataaat gcaacaaatt
     8641 tatccataaa tcttccctct tttctttact gtagattacg cgttcaatcg acgatacaga
     8701 tcaatgatct cttttgctaa atctgtaaaa gctatagatg tcatcagatg atcttgactg
     8761 tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg cttcttgtgt cagcatctct
     8821 gtttgagagt gatgtgcttg gactaaagat tcctctgcat ctttgatttt ttcatctgct
     8881 aaagtgaaat cacctttttt cgcagcagca atcgcttcca ttgcatcgct ttttgcattt
     8941 ccgccaaaca tgatcagacc catgatggct tctagatttt tttgatcttc catttattgt
     9001 atcctccgat aatttttatt atttgtttat tagtttttct gcttggtcga gtactttttc
     9061 accattcatc atgccatagt cagccatatt gattacatct aaaggaatgc ctttaggtgc
     9121 tagcttttca gcaaattgat tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc
     9181 tacattttta gattctagtt gattatccgc atctgaagca gatacagcaa agatgtctgc
     9241 ttccattcct tttgcttctg ctgccttttg catttttgtg actaggagac ttgtactcat
     9301 tccagctgaa catacaagca tgattgtttt cttttccatt ttaaacacgt tcctttctat
     9361 acttacactt gtatataagc aagtagcgtg ccaactttaa aagcgcttta aaaagttgat
     9421 atcatcacat ttaaggtcta taccaagttg catacacact aaaaaagaaa gcgcacacta
     9481 aacacacaaa ttaattttta ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac
     9541 acaaaacttg tcacaacttt tttcaaataa acggtattca aaagcttgca tctgcggtaa
     9601 taagtccaac caagttaaaa acataagatg ttgtttttac tttgctattc ttattacttg
     9661 atgcaggaca gctttttcac aacctctcaa taaacggtgt tcaatcagct gaccctgggt
     9721 attagctcaa aacgctggat tcacaacctc ttttatttca aataaccagt aaatacaatg
     9781 aacatctaca aaaaacttct atttatttat agatttcgtc aggcaaatcc aattctctct
     9841 tatcaatcaa agtctgatac cacgaagcag attttttcaa tgttctgctt cgttcatcgt
     9901 tcaattcgat tcgaactaat ccatatcgat tcttaaatgc attcattggt gacacacagt
     9961 cagtgaatgc ccataacata tagccttcgc aatttgcgcc ttcttctgtt gctttgatca
    10021 agtaatgcaa atgctgtccg ataaattcga tacggtaatc atcttgcacg attcctttct
    10081 catccatata ctgttcttca tattctcttc ccataccgtt ttctgttatc agccaaggga
    10141 tattatgata attttctttt agatacatgg ctatatcata catgattttc ggatagattt
    10201 cccatcccct ggaattattc attttcttcc caggtaaatc aaagacttca taatatcgtt
    10261 caggatggaa aggtgtctct tcattccatt gataacgagg ggcgcgaacg cgtttaggat
    10321 aatattgatt gattccaagg aaatctactg tatgatgctg aatcgtttct aaatcctctg
    10381 ttgcaggatc gaaataaatc gattgttttt gacacaactc caggaactct tctgtgtagt
    10441 atccttttac catcggatca aaaaatactc gattataaaa caaatcatac atataagcag
    10501 ctttttggtc ttctggagaa ttggatcgag catagaccat ttcaggattt aaaatacagc
    10561 caatccttcc accataattt ccgctgcggt aaatctctac tgctttagcc gttgctaaaa
    10621 ctttatgata attccacatc atccactttt ttgtgttttg ttcatgcggc cagcgaacag
    10681 catccaaata gcaacgcgtt tggggaacga ttggttcgtt gaatgtaaac cagtttttta
    10741 ccctatcacc gtaacgatca aaggcaattt gagcatactt agcataaaaa tcaactactt
    10801 ttcttgaaga ccagccatca tatttttcca aaagaaaagc tgggacttca taatgttcca
    10861 aacaaatcat cggttctacg tctgcttcga tcaaagagtc gatcatttca cttatatgct
    10921 gagcatattc ttcatctact atacagttct cataatcagt aaaaaaacga gcccaattga
    10981 tagaagtccg ataatgtgtc agatggatgg atttcatcag atccacatcc tgctggtatt
    11041 gttccataaa attagttgct acggctgggc catacccatt atgccaaaca aaatgttcat
    11101 ttttatacca gctatctata aacgaatcct gtccttcttt ttttcctttc caaccttccg
    11161 tttgccaagc agaagatgca gcaccaagaa taaaattttt tggaatagta attttcatga
    11221 tttcctcctg ttttttagat cccatcattt tccacacgat ttgctacttt tacaaatggc
    11281 agatagatca aaatagaaac aataatacaa acaagttgcg taataactgc accgatattt
    11341 cctgctgttg ccagataagc attgacgatc ggcggcattg gccaagggac cattacaact
    11401 gctttcgccg caaatcctaa agcggttgca aaataaccaa tcgttccagt gataagaggc
    11461 gttaagataa acggaatagc taaaattgga ttcagcatga tcggcatacc aaaaataact
    11521 ggttcattga tgttgaagat cccaggagca agggataact tggtgatttg tttcatatct
    11581 tcccgttttc caactaacaa tactgcaatc agtaaaccga tcgtgactcc agacccgccc
    11641 atactcatat acatatccca aaatggcata gtaataatgt tcggtacttc ttttccagct
    11701 tcaaaagctt ccgtatttac agcaattgcc gctaaaagca acggttcccg tatcggcttc
    11761 accatttgat ttccatggat accaatcacc caaaagactt gcgcaataaa catcaatagc
    11821 aaaatacccg gtaacccttg tacgacacct tctaatgggc gttgtacgac attgtaaatg
    11881 atatcataaa ggtacattcc tgtaagttct ttgatgataa atcccgcagc cgcgataccc
    11941 gatacagtca gaatcgtcgg gaataaagca gaaaaactag cagaaacatt cgttggcacg
    12001 gtatcaggca ttctcagctg tagtttcttc tgtttcctca accagcaata aagttcaata
    12061 gaaaggatgg agaccaacat tcccaaaaat aaccctttcg tatctgtata ttgtttagcc
    12121 aatacgtttt ctactataac agtctgattt tcattcacta attcataaac agttggatta
    12181 acagataaat aagacatcac tgcgatgata ccagggaata caccatccac tttattcagc
    12241 ttggctaatt cgatccctat cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca
    12301 tagctgatgg cagatgcgat aggctttaat tcttctaaaa aacgcaagaa acttatctgt
    12361 gctaagccat tttccgagtc aaagaccatt gctgaaaaca atgtgccaaa agcacccgta
    12421 ataattaccg gaatcaatgc agcaaatgcg ttttttatca ccatgatata ccttaaatta
    12481 ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt tatccataaa actcattcca
    12541 gatcccctca ttcctgcttc ttctccttga cgtcatctct ttttgtctat tagtcagcgc
    12601 ttacatttcg atgaacagaa actatttact cctctttttt accaaatata aaacaaaaat
    12661 tcatctgatg tatttatcat aaaatacatc agatgaactg tcaattagtt tctttttctc
    12721 ctattatttt ctgtaattct ctccgattta ttgccatatg gacagtcatt gcatcaaacg
    12781 cagcaagtgc attccgcctt tcgatggcct gaacgatctc tgtatgaata tcgatcgtct
    12841 cgattttcag atgctggata tcataatttt tattaaacaa agcaattgat tcattaatga
    12901 ttgggataat atgcagcaag gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat
    12961 cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg
    13021 ttaatttttt catttggacg atttgattct tcgttgcata ttccgctgcc aaacctgcca
    13081 tcttaggctc caataaatat ctcagttcaa ataagtctcg tatcatccga tcctgatctt
    13141 caatcaaaga aaaaccgagt gggtcattgg caatccctcg cttatcactg acaaatgtcc
    13201 ccgctccttg ccgaacttca aggatattcc tggaagctag agcccgcaca gcttctcgca
    13261 ccgtgcttcg ccccacctct aaataatcgg acaattcata ttcattcggc agtttatctc
    13321 ctacttgcaa ctgcatttcc tttaaatatc taaggatctt gtcagtagtt acttctacta
    13381 gggttttatt ttcttccaaa ccacatacct ccttttcatc tgatgtattt tttcgtaatt
    13441 attataacac gtttgaatca tctgatgatt ttttgtatac aaaattgatt aatccgttca
    13501 tagacataca aacgaagaag atatttacca taaaaaagac taggataaaa ttcactccta
    13561 gccttcgatc atttttattt tgaacgacaa aaaatatgat gttcaataac agacgatctt
    13621 cattcgattc ttgatgtcat cttttgtttt ttttaatata tggataaaaa gtttgattac
    13681 atcgtttcta ttctagaatt tggtcgattc tcaattcttc ttctggtgag aaatctaaat
    13741 tatccatcat tcgaacactt tcttgtaact gacttaaccg acttgctccg accagtacac
    13801 ttgtaacaga tttttgtcgc aaattccatg caagggccat ttgcgctaat gattgtcctc
    13861 gtgatacggc gattgtttgc agtgctttga ttttttccag agttgatcca acttgttctt
    13921 ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc ttcaggaata ccatgtagat
    13981 aacgatttgt cagcaaccct tgagcaagcg gactgaacgt gattgcccct agcccttctt
    14041 cttctaatac tggagataga tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca
    14101 tctgatgaat aatgaacggt gcttcctttc tttttaaaat ttcagtcatt ttttttgtat
    14161 cttctccatt atagttggat ataccaatat ataatgcttt tccttgtctt acaagttgca
    14221 tcaaagcttc tgctgtttct tccaaaggtg tatcagggtc tgggcgatga tgatagaaga
    14281 tgtcaacata atctagcccc attctttgga gactttggtc acaacttgaa atgatggatt
    14341 tacgagagcc ccattcccca tatggccctt cccacatatg ataacctgct ttgctagaaa
    14401 taatcagttc atcacggtag gcttgaaaat cttctctaaa aatacgccca aagtttttct
    14461 ctgcagctcc agcagggcct ccataattat tcgcaaggtc gaaatgggta attcccatgt
    14521 cgaatgcgcc tcttaaaatc tctctttgat tatggatagg atcataatca ccaaaatttt
    14581 gccataatcc gagagataaa atcggtaatt tcaaacctga atgaccggat ttacgatatt
    14641 ccattttttc ataacgtgca ctatctgcaa tatacaattt ttctcctcct catcaaatct
    14701 ttttaaaacg ctttcttttt tctattttta gtttactaca ttttaaagaa aaggagttaa
    14761 tcaaaatgat tttttcgtat atttttttgc catttgtttt ttactcgttg atagatcaga
    14821 aattgattac gtgcttgctg gattttttta taacgtttcg gttgtttttt gtaaaattgc
    14881 tgatgatagt tttctgctgg ccaaaacgta ccggctggtt cgatttttgt gacgattggt
    14941 tggtggaaag ttcctgaact gtctagtttc tgttttgatt gttccgctaa ttctttttgt
    15001 cgttcgactt gataaaaaat cactggacga tattgcttcc ctctatcttg aaattgtcca
    15061 aatgtatctg tgggatcagt aatttgccaa tagattgtca gcaactcgct ataacttatt
    15121 tctcttgtgt cataaatgat ctccacagct tccacatgac ctgtgtaacc accacttact
    15181 tgatcataat ttggtttttc aacatgtccg cccgtgtatc ctgacaaaac agataggatc
    15241 ccttttttcg attcaaacgg ctcgaccata caccagaaac atccccctgc aaatactgca
    15301 cgttcttgat agtcagcatc ttgcatggca tgcttggaaa agtcatattt ttgctcctta
    15361 tcaaatcctt ccgtaatcaa taaataaaag tccattacat caggcgtcag attcattcgg
    15421 atagctaatg gacgtagtgt tgcttctagt tttgataact gttctttaac ggatatattt
    15481 tctcttatgt cattctttgt ctgaatcaat acttttcgtt cccaatctcg tgtattcgga
    15541 ttcaaaatca gattataaag tgtttgaatg aatatttgtt cctcatccat agattctcct
    15601 cctttccgaa gaagacaagc tagtgacttt agctgtaatt tatcataaag tgaggaaata
    15661 agcgattact tcatccccct acagattttt cgtataaaaa agagagaatc cttatcgaca
    15721 gattctcctt tttaatcaat ttattttata aatgaaaagt taatttacct tttatggaaa
    15781 actattttaa aaaataaaca taaatatgca gttaatgtaa caagataaga aataatagtc
    15841 gttactgtat aacttgtgct tactttaata tcagcaactc ctttgactcc gcttatttgt
    15901 accaatccat tagcattcac ctgctccttt acaggttgat gatttatttc tacattcgtt
    15961 cctaaataag catatacagg aagtgtgatt acttggctct tctcattagt tactctatat
    16021 atgatagaac taccagttgt tttataagga aggtttaccc attgactgcc aatctttgct
    16081 tcgtgtttta ttatatgctc cttattctcc aatgaagcga taggagaata atcatataaa
    16141 aaataatcca ttcctttagt ggtatacgcc tctactgttt ctttgtttac aacaatatgt
    16201 cccggctgag aaattatttt ttgattgaaa ttttttttac tagaaacagt taaagtgatt
    16261 aataacaaaa cagtcataaa taaataaaat agttctcttg tcttattgta tcttctattt
    16321 ttgaaaaata tcatcgttaa tatcagagaa gagaatatta tttgcaagct taagatccgc
    16381 caaggaaatt gtaacaattt ggctggcgta ttttgcagaa gataccatgg caccaaacta
    16441 gttgtagcta gccaagtcaa acacgtacca taataaatct ttttttctat gtttgttaat
    16501 tttttaaata aaatcagggg aatacataaa gataacagac caactagtcc cattgagtaa
    16561 gtagttaagt cgttgttcat agcttcagtc agactatcga acacattaga tgcatgagca
    16621 tataaatttg tcacgctagg tttgttgatt ttttggtaga gcatttgttc aaacatcgga
    16681 taccaaaaag cactcgtcag aaccaatgtc attcctgccg cactaaataa ttttttaaat
    16741 atagcaataa aatctgcatt cttgaaacct ttagaaacca aacgaataaa aataaaaatg
    16801 ataattaatg ctgtactcat taatgttgat agtacatggc tgtacactag caaactcata
    16861 ctcaatgcta gctttacaga cggatacttt tcttcttttc cagagatgat ttgataagca
    16921 taaaataata cagggataac aagtgttatt gcgatagctt cggcaattgc ggaacgatag
    16981 tagatgtcta ctgtccgata agcagagaaa atatagagac aactaaataa aaatgccgct
    17041 tttttttgtt ttaaaaaccg ctcgccataa tgataagcta cgagtgttgt acacaagttc
    17101 aataaccaaa cgtaaataat atagcttaca atcaaatttt ttgtcatcca atataacatt
    17161 acagctggaa aaaaagtcag aaagggataa aaataattta caccatatcc agtataattg
    17221 aatgtctcat aattaatcgg actggtaaaa atagtcgata accccttgat tcgagataag
    17281 tgaaaagaaa gatctccagt ggtataaata ctctgtgtat ttttgaaaaa gatgaaagaa
    17341 tatatactac taactataat taatccactc aatattacta cttttttatt tattttcatt
    17401 cttcttgttt atctcctttt tagtcacata tcgagaatct ttcaacttat taggtgcaaa
    17461 tcgtttaaaa cttgaataca ccactaaaca ataagtcaga atactgatcc ataaagccat
    17521 gagagcagtt ggagtatatt ttgttgtaat ttctatctta ttttctccag catttacatc
    17581 cacattcact aagccatgtt ttttataaga gtcatttacg actttaccgt ttacttttac
    17641 ctgtgtacct aaataagcat atactggtac tgtcactttt tgctttttgt tgcttgccac
    17701 tgtgtaagta atgttattat ctgcagcttt atacttccct ttttcccacg attctcctac
    17761 ttttacttca tgtttttcta agtgaggcct ttcttttatc gcttctgttg gggcgtaatc
    17821 gtataatcct cccatatctg atgttgtata acgtgggagt gtttcttcat tgataacaat
    17881 atgaccgtgt tttgattgga taacttttgc gtaattttct tttgctgcta tagacgtgac
    17941 gagtagaaac accacagtag cgccaatgat taaccatgaa tattttttat tcatagtctt
    18001 tttcataaaa acaagcgtta tgatcattga agaaaaaaag atttggatac ctaagatacg
    18061 ccaaggaaac tggagtaagc ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt
    18121 agttacgcat acccatgtag aaactacgcc aacatagatg attttttctt ttttctccat
    18181 ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta
    18241 tgttgtaata tcgttattta acgcttgaat gatactatct gaaatagaaa gtgcttctag
    18301 ctgcaaatct gttttgaagg gactgtgtat ttcttgataa ttcatctgct gcaacatagg
    18361 ataccaaaaa tagctagtta atgcacaagt caaaaaaaca gcctttacta aattcacgac
    18421 cacaaatttt atatcgtttt ttatttgctt gcttaaaaac aaacgaacta taatcataca
    18481 agcaatcaat gcactacaca atagtgtagt taagacatga gtatacacaa gtagacttat
    18541 actgacagat aaagcgatga cttttttttc tctattgaat atcaatccat aagcataata
    18601 taaaacagct gggataatcg taagagcgat tgcttctgca attgcggatc gataatagat
    18661 atcaactgtt cgataagttg caaaagtata taaacaacta aataaaaaag cagcatctat
    18721 ctttttaaaa aacttttctc cataataaaa actaatcaga atcgtacaaa tatttaataa
    18781 ccaaatatat agaacataac ttaatattaa gttatttgaa attccataaa aaacgactgc
    18841 aggaattatt gtaagaaaag gataaaaata gttcagtcca tctccataat tgttaaatgt
    18901 tgtatagttg attggccctt caaaaataga agataaccct ttgattcgtg aaagatgaaa
    18961 agataaatca cctgttgtat aaatttcttg agcatttctc ataaaattta ctgaatataa
    19021 agtactaagt aagataagaa ctactaaaag aaatgctttt ttacttattt tcata
//
LOCUS       DAYYHS010000059        18508 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000059 DAYYHS010000000
VERSION     DAYYHS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.059"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..181)
                     /locus_tag="ACHA6A_001961"
     CDS             complement(<1..181)
                     /locus_tag="ACHA6A_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8970131.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAR"
     gene            complement(245..913)
                     /locus_tag="ACHA6A_001962"
     CDS             complement(245..913)
                     /locus_tag="ACHA6A_001962"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8970132.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(925..1365)
                     /locus_tag="ACHA6A_001963"
     CDS             complement(925..1365)
                     /locus_tag="ACHA6A_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970133.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(1424..2095)
                     /locus_tag="ACHA6A_001964"
     CDS             complement(1424..2095)
                     /locus_tag="ACHA6A_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970134.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(2280..2678)
                     /locus_tag="ACHA6A_001965"
     CDS             complement(2280..2678)
                     /locus_tag="ACHA6A_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8970135.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(2675..3448)
                     /locus_tag="ACHA6A_001966"
     CDS             complement(2675..3448)
                     /locus_tag="ACHA6A_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8970136.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(3445..4128)
                     /locus_tag="ACHA6A_001967"
     CDS             complement(3445..4128)
                     /locus_tag="ACHA6A_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8970137.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(4295..5182)
                     /locus_tag="ACHA6A_001968"
     CDS             complement(4295..5182)
                     /locus_tag="ACHA6A_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970138.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(5179..6048)
                     /locus_tag="ACHA6A_001969"
     CDS             complement(5179..6048)
                     /locus_tag="ACHA6A_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970139.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(6185..7471)
                     /locus_tag="ACHA6A_001970"
     CDS             complement(6185..7471)
                     /locus_tag="ACHA6A_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8970140.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            7744..8322
                     /locus_tag="ACHA6A_001971"
     CDS             7744..8322
                     /locus_tag="ACHA6A_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8970141.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            8322..9251
                     /locus_tag="ACHA6A_001972"
     CDS             8322..9251
                     /locus_tag="ACHA6A_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970142.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            9248..10984
                     /locus_tag="ACHA6A_001973"
     CDS             9248..10984
                     /locus_tag="ACHA6A_001973"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8970143.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(11047..11883)
                     /gene="pheA"
                     /locus_tag="ACHA6A_001974"
     CDS             complement(11047..11883)
                     /gene="pheA"
                     /locus_tag="ACHA6A_001974"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8970144.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(11880..12389)
                     /locus_tag="ACHA6A_001975"
     CDS             complement(11880..12389)
                     /locus_tag="ACHA6A_001975"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8970145.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(12382..13677)
                     /gene="aroA"
                     /locus_tag="ACHA6A_001976"
     CDS             complement(12382..13677)
                     /gene="aroA"
                     /locus_tag="ACHA6A_001976"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8970146.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(13690..14781)
                     /locus_tag="ACHA6A_001977"
     CDS             complement(13690..14781)
                     /locus_tag="ACHA6A_001977"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8970147.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(14798..15964)
                     /gene="aroC"
                     /locus_tag="ACHA6A_001978"
     CDS             complement(14798..15964)
                     /gene="aroC"
                     /locus_tag="ACHA6A_001978"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8970148.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(15966..17030)
                     /gene="aroB"
                     /locus_tag="ACHA6A_001979"
     CDS             complement(15966..17030)
                     /gene="aroB"
                     /locus_tag="ACHA6A_001979"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8970149.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(17063..18085)
                     /gene="aroF"
                     /locus_tag="ACHA6A_001980"
     CDS             complement(17063..18085)
                     /gene="aroF"
                     /locus_tag="ACHA6A_001980"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8970150.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         5312 a         4057 c         3245 g         5894 t
ORIGIN      
        1 ctctggcatt caagtttgat tcattgatca aataactagt agaatacttt ttcattttga
       61 agtagatttc ttttttgtcc atactgatac caaaatcttt tagtgcttgc catgcaccat
      121 ctaccattgc gggatacgta tcaaacaatg taccatcaaa gtcccatata taatttttca
      181 tgttcctttt gttccattcc tttgcttctt ttttctatct ctcactttta gataaaggtg
      241 ttctttaaag tatttttttg tccagaattg tcaaatcatc tgctaatgta tactggattg
      301 gttgtgcatt tgggatatcg atcgtatcca tttgatcttc aggaacatct tctaaatatt
      361 ttgtcagtgc tcggatgcta ttaccatgac ccacaactag taaatcttgc cgatctttta
      421 gttttggtgc tagatgatct tgccataagg gaatgatccg attcactgtc atttccagac
      481 tttctccgta tgggatcaaa tgcggatcca agtgatcata tctgcggtca aatctatttt
      541 ctgcggttaa cggcggaact tccgtatatc ctctgcgcca tgtttttact tgatcttttc
      601 cgtatatttc tttcatctta tctttgtttt tacccacaag atccccataa tgccgttcat
      661 ttagacgcca tgtttttatt ataggaagcc ataactgacc catttcttct agtgcgactt
      721 gtccggtaag aatcgcacgt ttcaaaacag aggtataaac ggcatcgaat tgaagtccgg
      781 cttcaagcat tttttgaccg gcaactgctg cttgttcttt tcctttttct gttaaggcga
      841 catctagcca tcctgtccag taattttcaa aatttgcttc gctttcgcca tgacgtaata
      901 atactaattt cattcttttc tcccttaaac attcgtttcg aattttatac cacatgtttt
      961 gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc tgcttttgat gacaattcgg
     1021 gcactcaaaa aaagtataac tttttcgatc cattttgact tgctttttgt tttttatctg
     1081 atgatatttt tctttgactc tatcaagata tttcaaataa cgttgattct catttagtct
     1141 ggggtagatt ttttttgata ggaaacgaaa tatcatccat ccgaaaaaga cgatgaacaa
     1201 caattgtccg attctatagg gcaaccaacg acttagcaaa ctaaagacaa gcgcagcgat
     1261 caatagaccg cgattcaact gatcaaattt cgcatatctt ccctgcataa gtttgtgata
     1321 gttctgctga aatcgtatta gcctttctaa ccatgtttgc atcatattat ctcctccatc
     1381 atctaatatt tattatgaca aaaaaatcca aaaagaaaaa gccttaattg gccttttcca
     1441 ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc aatcacaatc actttctttt
     1501 tatcttctct gttgactact cgaaatgtgt ccgtcaatgg aagaaacagc ttcttagatg
     1561 gtagctgttg catacgtata aaacgatgga tcatcgtaat caattgagga aattgaatag
     1621 acgtgtgaag cagacctgga tattttttca acagctgtgt cgttttgatc agattccgtt
     1681 ttttactgat cacttttaaa aagcttttta gaatcttttg ctgacgtcta aaaatagaaa
     1741 aagaattttc gtcatctatt tgataacgca tttgtttttg cagttctgct gcagtcagat
     1801 ggatcttccc atcgtttgta tcgatcatta aaccttctgg aaacagttca accactgctc
     1861 gatcccaaga ttcaagagaa agctcgatat aatcttttac tggtaattcc aattctacct
     1921 taaaaaagtg caaaacagcc gacagccctt ctgcttgata gatttctcct aatgttagcc
     1981 cctctagatg gaactctggt gaaaaatgca gcaatgtttt attttcctgc aattgaacat
     2041 atagaaatgt aaatcgctca atcagagtat cttttaaaac aactaaaaaa ttcatttttc
     2101 tccccctttt ttcatcattt ttaaaatagc atattttgaa aagaaagtct gatgcttaga
     2161 gtagaatttc ataaaaattt actatttcat aagtattcac gagaaaaata caaacaaaaa
     2221 agcggaaata acgctcatcc gccttcttgt ttgtgtttat ttcctcttca aacgcttgtc
     2281 tatgattcag acttttctct atcgttattt tcatctaaaa gttcttgcat tccgataatt
     2341 tctcgatatt gatgaatctt agtgatacca atgactttgt ctacatcaat actaaaacaa
     2401 ataccattac ctggaatatc gatgttgatg ccttgaccaa tcgcatccat tactttattc
     2461 gtcaaactat cgggtactaa tgtcaacaca atatcttttt ctggcgaaat gtccaaacct
     2521 aaaaatttcc ctgaatcatg tacaccagaa cctcttccat gtaagatcgt tccgcctgct
     2581 gcacctgctt cttttgaaaa gtcgataaca tcttcgccca ttcctccgtc aacgatcgtc
     2641 acaatcatgt gaagattcaa tgctacaaga tctttcattt gctttccccc aatttttgca
     2701 atctttcttt ttcgattctg tctttttcat cttttcgttt ataaatatat cctagtacta
     2761 aagtggataa tattggtgtc aatgcaatca ttgcaaccac accaaacccg tcaatgatcg
     2821 gatctcttcc ttcaatttgt gcagcgattg ccacgctcat tgataaaata aatgtcgaac
     2881 acatcgggcc cgttgctact ccaccgttat caaaagccat tgctaaaaag atcttgtcca
     2941 cttttcgtcc taaaataaat gctagcccgt atcctgggat caagaaataa taaagagaga
     3001 atccgacgag caaacgccac atagacaatc ctactgctgc gccgatccca attgaaatta
     3061 ccatcagcat gattttcgat cggattctcc cctcgctcaa ttcttcaacc tgtttgatca
     3121 ttacatgaat cgccggctca gcaaacccta ctaagaaccc cattaaaaag ccaaggggaa
     3181 ccagtatata attgtaaggc aatcccgcaa tagcactgcc taaatgctgt cctaccggta
     3241 cataagccat attgactccg tgcaggaaaa ggatcaaccc aatagtcgtt atcaagaaac
     3301 ctttcataat gtcaaagaaa cgctttttgc gcatacgaaa actaatgata ttcattaaaa
     3361 caaataggat tacgataggt gccactgcca aaatgacttc catggtaacg atttcgattc
     3421 catctatcac ttgctgccag aaactcattg gaagatcacc cccaaaatca tcacggcaag
     3481 aatcggccct aacgaagcaa taccgaccat tccaaaactg tcattttcat ttttcttttg
     3541 tttgatcata ctagtcatcc ctccggcaag ggccaagata aatggaacag ttactggacc
     3601 agtagtcact ccccccgaat caaaagcaat cggcataaat tcggaactgg tgaaaaatga
     3661 ggcgatcaaa acgcctatgt agcctatagc catcaattga taataggata gcttgaatac
     3721 gacccgaagc agcgcaaagg ctagaaatac tccagtgccg acgctgacga tcccaatcag
     3781 caacactctg ccaatctctc cgtcagatat atcatatact tgttgtccca aaacttgcac
     3841 ggaaggttca gcaatcgtga tcacgatccc gatagcaaat cccaaactga ccaaaacggt
     3901 caaacttttc tttttgatca tatatttccc tactagatct ccgacttcca tcatagaata
     3961 atctgctcca aacaaaaaga gcgtcattcc gatcatcatg attgccgcac caaccagaaa
     4021 tgaagcgagg tcttctcctt ctaacggtgc aaaaacaaac gttaaaatga caatcaggac
     4081 agtcatcggc aatatagcag caatcacttc tttaaaattt tttagcatgt atcctcctcc
     4141 tttactcttt aagtttacct taaaatgaaa gagttttcga gcacaaactt aaaacgttat
     4201 catttaattt ttctcaacat aaaataacac acgtcccaaa agtatctttt tacttggaaa
     4261 aatgactttg aaggaacgcg cttattgtgt ttttttattt tacagagccg atcattcctt
     4321 gaacgaattg cttttgcaaa acaaaaaata cgatggcagt tggtaaagta gcgatgacta
     4381 atccagccat gatcattcca tagtctggtg tataggaagc acccatagca gataaaacaa
     4441 gcggaaccgt ccgtttatca ggagattgca aagctactaa gggccataaa taattgttcc
     4501 agctactcat gaatgtgatg attgcagccg cggcataggt atttttagcc gtcggcatat
     4561 acactctaaa aaaaatactt agttctttta gaccgtctaa tcgagcggct tctactagat
     4621 cttttggaaa agatttaaca ttttgtcgaa agaagaagat caaaaaagca gtgctaacag
     4681 atggcaggat gacaaccaag aaactgttga ttcccaaaac cgttccgttc aattgtgaaa
     4741 acattcgata taaagggatc atcaatgccg caaaaggaac catcatcgat agcagtagaa
     4801 tattgaaaat taggtctttt cgtttacttt gataaatctc aaatccatat cctgccattg
     4861 acgaaatcag caatgcaaac acagtggtca ccaaagcgat catcgctgaa ttccaaaggg
     4921 aacgagtaaa attcagctca ttggaaaaca aattttgaaa attgatgatc aagttactgc
     4981 caatctttat ttttcccgat gtgatatcca cgggtgtatt cgtcattccc aaaaccatcc
     5041 agacaaaagg gaaaatggaa ataaatgata ggatgcttaa tgctgtatac tttaatactg
     5101 acccaattgt cgaacgaagt tctaatgctt gttttttgac gttggggcgt tcgattgttt
     5161 ctttttctac catatttttc atcgactttc tcctcctact ttcgtttgga ttatcgacaa
     5221 tatcacaata aaggaaacaa taacaaagga aactgctgcc gcatatccaa aattaggcgt
     5281 gaatttataa ctcaaattat agatgtattg agatagggtg gtcgttgcat ttgctggtcc
     5341 cccgccagta atattttgta cttcatcaaa aagctgcaat gtcccgatcg tcgaagtaat
     5401 ggaagtaaac aaaatgattg gttttaaatt tgggatcgtc actttgataa actgctgcca
     5461 tttagaagca ccgtcaatag atgctgcttc ataaatctcc gaatcaatat tttgcatgcc
     5521 tgaaaggaaa aaaatcatat tgtatccagt ccaacgccaa gtgatcgaaa gaatgatcag
     5581 aactctcgcc caaattggat gagtcaacca gccaatcggt gtgtcaatca taccaatgcc
     5641 caacaacaag ttgtttacaa ggccagattg agaaaataga cttttcatga tcagagaata
     5701 actgaccatc gaagtgatac atggaagaaa gatagcggtt cgaaacaacc ctttatattt
     5761 caacttttta tcatttaaaa ggttggaaat aaaaagtgct agaaacaaca tgatcggtac
     5821 ttgtacaagt aaatacagga atgtattaaa aaaagctttt ttaaaagttg catctgtcaa
     5881 catccgctga taattaccta acccgttaaa tgtcatattg acagggggac cactttgaaa
     5941 agacgtgatc aaagccaaaa ccatcggaat aaaaaccatg attgatatca aaatgactgg
     6001 caagattaaa aaagcatgcc cgtttttgaa taaatctttc tgcttcattc ttttccctcc
     6061 aattctatca ttgattatct acatgaaaag acaagaaaga caatatctgt aaggcagacg
     6121 tgatcttttt tgtcttttcg tttgtctgat gaaaaagcga agaaattcac ttttttacca
     6181 ccgatcaatt agccaattgt gcttctactt gtttttgtgt atctgctagt accttgtctg
     6241 tctcttctcc attgatgatt cgatgaagag cttcagctac gactgcttca attgcatagg
     6301 tctcatgtcc gtaattcact tctggtattt cagatgtcca tgttgaaaaa tcgtcaaaga
     6361 tcttttgacc actgtaaaat tcagaagctt cttgataagc ttcttgttca ttcgcgctaa
     6421 gcatcgtaga tactagtccg atttctttcg caagagtgcc catcagttct tcattcgaag
     6481 caaacgttgc ttctaggaaa tctttcgctg aatcttctcc agctactcct ttgatcacat
     6541 accagccagc gccgccaagg ttagaagctt gcgctttttg cagattttct ggaagtgcag
     6601 gtattggtgc aatcttccat tttccagatt ggttttctgc tccttggatc gttgaagaat
     6661 accaagcgcc agttgggcta gaagctactg ctcctgattg tatcgcagtt actccagcat
     6721 tccagtcaga ggtttgttcg actagatctt ctttcaaaag agtggcaaac aattcaagac
     6781 catactttaa agatgcattg tcttggatcg tgactgtctc cccatcttca gatgtatacc
     6841 attctcctgc ctgttgcata atcatcctta ctcgaccaag gtcagaagga ttgacttcag
     6901 cgattttctt tcccgtcttt tctttgacat ctcgcgctac ttgaatatac tcgtcccact
     6961 tcagattctc catatcctct tctgtataac ctgcttctgc catcagatcc gtacgataga
     7021 agtttgctgt tacgcccgaa tcgaaaggaa caccgtatat tttatctcct actttgttta
     7081 ccgcaaattt atacggtgca aaatgatctt cctttacaat agcagacaaa ttagcgaatg
     7141 catctggata agaagtcaaa tagccttgta tcctataatc ttcaatcaag acgatattgg
     7201 gcaatccttc tgtattcccg ctcgctaaag cggtgttcag tttctgaacg atatcatcct
     7261 gagacatcgt aacaacttct atctctgttt cttcgttctc ataaactttt tttgcttcat
     7321 tcactgcttt gatattgaat gtctcatccc acgcccagac agtgactttt tcttctttgt
     7381 ctgctgtact actagcactt ccgcatcctg ttaagaaacc tgatgctgcc gctaaacaaa
     7441 cgactccagc aattaacttt ctgatgttca tttttatccg ctccttttcc atccattagt
     7501 tgtttacgtt tgctacattc atcataccaa aatttgaaaa cggtttcttt attattcaga
     7561 tacttcaaaa atttctccta aaataataaa aaaatacaaa tcaactatga caaaaggaat
     7621 aatcttagca tctagaccaa agatcgaaat ggtgaggttt ctttaaaata aaaatatcct
     7681 tgcattttct cctagaaata agtattttta aattacaaga ctattcttaa ggcgggtaaa
     7741 aaaatgccaa tacgcgtaat tcttatcgat gatgaacaga cgattttaga tggaatgaaa
     7801 cagttgatca attggtctga gcacgggttt caattaatag ggactttttc cagtccgctg
     7861 aaagcgcttg atttttcttt ggtacatgca atcgatatcg tggtcacaga tgtaatgatg
     7921 cctgaattaa atggaatcga attatcgcgg ctattaaaac agcaacaacc tgaaacacag
     7981 atccttatcc tctctagcta tgatgaattt gatatggtta aagattcttt taaagaaggc
     8041 gtcgctgatt atcttttaaa gcctcgattg agtcctgatt tttttctaaa ttccttaact
     8101 gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc caatcaccaa tcgttatgag
     8161 cagatctgcg agaatctttc acagcagatt gctggacaaa ctcgtccatt gcttgaccct
     8221 cttctttttg aacacacctt gtttttctgt tatataccga aaaacggcca ttcagttcaa
     8281 aacaaggctc taaacaaaaa cagtcgatca cccattcatt gatggatagc gacgattcgt
     8341 ttactattta tcctgtttct accttttcgg aagaatccgg ctatgtaatc aatactgatt
     8401 ccaaagatac acttaggaag tttgttcaaa agtttcgtca agaagttttt ccaggtgatt
     8461 tttttgttgt ttctgattct tttgcaatcc aagatatcaa tgaaatattc caagagatca
     8521 gaaattgcac caaaggacaa cgtttctatc ataagggaaa gaaccttgct tatcagcatg
     8581 aacttcttcc gttattaacc aaccacgagc agcaagcaag tagttattta acaaaaatcg
     8641 ttcataaaga ctacgcttta gctgtcaaag aacttgagaa tttcttcacg acgatattgg
     8701 aacagcctat tgatccagct tatttgaaac atgaggcaat caatcgtttc tatgcattac
     8761 tcaacgcttt ggctgacgag catgttggga atgaagaact ttctttgctc aagatcgccc
     8821 ttccttcttt attagccgat gctgagtatt tcgaagattt ttctattctt gtcaaagaaa
     8881 cacttgagaa actcacccac ttttctgcaa atgcaaaaaa agacaatttc gatatcttga
     8941 ctttgatcta cgagttcgtc caggaaaatt atgagaaaga aatcaattta caacttttat
     9001 ccgaaaaata tcatttttca tatagctatc tctcagccat ctttactgaa aaatttggta
     9061 tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa agccaagctc tttttgacgg
     9121 aaacacggct tactttgacg gaaatctgtg agaaagtcgg ctataccgaa ctgggttatt
     9181 tctccagagt ttttaaagaa gagacaggaa tgacaccttc tcaataccgt aaaggaactt
     9241 ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt gccaaacttc tgttcatttt
     9301 gttcatcggt ctttttctgc aagccatcgt catttctttg tttatttatc atcgatcaag
     9361 ggatgcctat atccagctgt tcaatcaatc gaatgatgtg gtactaaaaa agattcaatc
     9421 tgaatttgaa agtttgaatg atacaatcga gaatacatta gccgcttttg attctaatcc
     9481 agctgtcaaa agctattttt ccaatgatcc agcccaacat atggaacagc ttcagcaact
     9541 aagaacaatc caaaaaatga atgactctct ttcaaaaatc catccaatga tcgattacga
     9601 tgtattgatc tttggagaaa atggaagaac ttttgtcgga aacgacatgc tcactgccgt
     9661 atctgccgat tctttttttc aatctgcgat tgctcagaga gtgaatgaac gtgctgctga
     9721 tacacaaatg ctttttgcac accacggatt gactcttcgc gataagaaag cacccagcgt
     9781 cttctttgtc aaaaaactga aaaatacatt gaatcacgtt tacggatatg ctgttttatc
     9841 gatctcatcc aagcaactag cgaatctttt tcaatctgta gtaaatcctg agatatcgaa
     9901 gatctccttc atgaacgaag aacagacgat catcgcatca aatgaagaaa atacaattgg
     9961 gagccgatca atgattttca aaaaactaac agtaggtgaa acaacgattt caaatggaca
    10021 gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca ttactcagtc aagtcgatct
    10081 tcattcttta tctaaccaaa tgggcgtgat ttttccgatt attttgtata atatcgtgac
    10141 acttgctttt gctggctcat tggtatttat gtatttggat cgacacacaa aatcgatcta
    10201 ccgcttgatt gattcgctaa aacggctaga ggaagatggc ttaaaagcta ctgttcctgt
    10261 acaaggaagc catgaagtcc aattgctagg gaatacgatc aatcaattgc taagtaatgc
    10321 tcagcatcat tatgaacaga cgatccaaga cgaaaagaag aaacgttcgc ttgaaatcca
    10381 aactatgcaa gcacagatac agccccattt tatttataat acgctgacta gcttgaaatt
    10441 tttgatttgg cagaaaaaaa cagatgaagc agttcaagga atcgacagtt tcattacttt
    10501 actaagaagt acgattggga aaaaagaaga agtgattcca gtcaaggaag aaatcaaaag
    10561 cgtacaaagt tacatcgatc ttttgagtat gcgatatggc gaaagaatct cggctaaaat
    10621 aatgattcct gatgaactgc agtcattatc tatgccgaat atgatccttc aaccaattat
    10681 cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa ggattcatca cggtgtacgg
    10741 caaaacaaaa aatcagcttc ttttattcga agtgattgat aacggcgatg gcttcgatac
    10801 tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc ggcatcagcc atgtagatga
    10861 gcggatacgc ctaatgtatg gcgatgcata tggcttaacg atccaatcag tgattgggac
    10921 aggaacgact gtaacgattt gtctaccgct tcttcctaaa aatcaatcag aatatcaaaa
    10981 ataaagaata gccaagcgct gattttttta ttttaagtta aataaatcaa tcagtattct
    11041 tgactttcat ctacgcgtct cttctactgt cagcactgga taacagccca atatttgact
    11101 ttctcctccg agcatcttga tttcttggag cgcattctct actaatgtca ttggacggtc
    11161 caacaacaaa tcgatcacga aaaaatattc tcccaagcta gttttcaatg gtcgggattc
    11221 aatcttactt aagttgattt cccgccagcc aaaagctgcc agcattttat gaagcatccc
    11281 tggccgatta gcaggtagtg tcaagatgac actcattttt actggagctg actgctggct
    11341 ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg ttcaactcat tatcttggat
    11401 attctcagct aagatctcca atccaacatg ctgtgctgtt tctaatgaag caatcgctgc
    11461 tgcgtcttct ttcggatgtt ctgctacata cattgctgca gcagttgttg attctgttgc
    11521 tactaaagga acattaggat aatgcgtttc taaaaactgt tgtgattgtg ctagtgcttg
    11581 tggatgtgaa aggatttttg taatctttgt tgctggattg cctaaaagct gctgcttgat
    11641 aggaagaacg atttcacttt taacttcgac ttctggatgt ttggaaagaa gatcgattgt
    11701 gtgatgaaca gctccttcca atgaattctc tacaggaacg acagccagat cgacttgttt
    11761 gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac gcagtcaatt gttctgtagg
    11821 aaatagctga caagccgcct gataagtgaa tgaactttct ggccctagat agctgacgtt
    11881 catatgcttt tgatcctttc tataatggta tcgatgatct tttctggtgt ttgattggtc
    11941 gtgttgattc gatgtgtcgc actttcttca taccaagaaa gtcgttgtgc caataatgct
    12001 tttatttctt ctcttgtttt tccaagagct aaaggtcgaa tagacgtttc atcttgttgg
    12061 atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa gaacaatcgg ctgtgttttt
    12121 aaaaaacgcc gattttctgc actttggaca atccctccac cagtcgcgat aattttgtct
    12181 tcatttgaca gctttcttaa aatatctgac tccacttttc gaaagctttt ttcaccgtaa
    12241 tagtgaaaat attcggcaat cgacaattgg atatgttcct caatctttgt atctaaatct
    12301 acatactctt tttgtaaaca ttgagctaac cctttaccga ttgttgtttt tccagcgccc
    12361 atgaaaccaa tcagcaaaat gctagccatg agcttctcct cctgacacaa gttctgctaa
    12421 atcgtcaaaa aatgccggat aagagattga aacagcttca gctccttcta gttcgcaagg
    12481 tccatcagct atcaaagcag ctacttgcag catcataccg attcgatgat cgcctctgct
    12541 ggacactttt gcgccatgca gaggtgtcgg cccatggatg atcagaccgt ctgctgtcgc
    12601 ttttatcttt gctcccattt tctgtaattc ttctgccacc gcatcaattc tgttggtttc
    12661 ttttactttt aattcttctg cgtctttgat gatcgtgatt ccttctgctt gagtagccac
    12721 taaagctaga atgggcaatt catcaatcag acgcggaatg atttctccac caatcgttgc
    12781 tttcttcaaa tgactcgtct tgacacttaa atcagccgat tggttctgtt cattgtgatt
    12841 cgtctgggta atcgctgcgc ccatctcctc caacacatct aagataccag ttcttgttgg
    12901 attgaccccg acatttttta acaacagttg gctttccgga acaagaagtc ctgctgctag
    12961 aaagaaagca gctgatgaaa tatcacctgg aacagttatc tgttgaccag ttaatttttg
    13021 cggtccggtc actatgatcg ttttatcttc cactgtgatc cttccgccaa attgacgaat
    13081 catttcttcc gtatggttac gggaacgttc tttttcaacg attacggatg tgccttctgc
    13141 ttgtaatgcc gcaaacagca aagcagattt gacttgcgca ctggctacag gcatattgta
    13201 tcggataggt gataaagagc gggttcctgt cacagtgatt ggtagatact ggtcattttc
    13261 ttctccttgc aagtcagcgc ccatctcgcg taaaggttcc atcactcgcc ccattggacg
    13321 tttagacaac gaagcatccc cgaacagagt agtcgtaaaa ggctgtccag ccaaaatacc
    13381 catcaataaa cgtatggtcg taccggagtt tcccatgtca attgattcgg ttggttggac
    13441 aaatgagtgg ctaccgcgac catgaatctt gatcacctct tcctcttctt cgatctcgac
    13501 acctaattgt tggaaggcat gtaaagtact cagacaatct tctgcgcgca aaaaattttg
    13561 tatagtcgtc gttccttcag caattgctcc aaacatgatg ctgcgatggg aaattgattt
    13621 atctgctggt atcctaacag tccctcttaa tccatgtatt tgttgtaata attgcattta
    13681 tccagcctcc tatttgatcc agcaatgata actggtatta gacataatga ttctttttgc
    13741 ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt attcctaaaa tatcttccct
    13801 ggtctcttgg atcttcaaat taatcaaaga aactcccgct tttccgatca gtccagttac
    13861 ttctgcaata acgccaggtt catctggcac atcgataaat aaatcaaaaa aggcaggaat
    13921 cgcgccttgc ttatggacag gcaactggtc tcgactttct tttgcccgat tgaaaaaaga
    13981 aaagatttcc gattggttct cagtcatgat ccattctgct atttgtgtca tttcttgttg
    14041 ccaatctgcc aatcgttcca ataatatctg cttattgctc aacaaaatat ctgtccacat
    14101 gacaggatct gaggaagcga tcctagtgat atctcgaaag cctcctgctg ccagttgttt
    14161 ggctcgcgga tactcttgat tgaattgttc tgcctgattg actaagccag ctgccagaat
    14221 atgtggaaaa tgacttagca ttcctgttat acggtcatgt tctcttgctg taagagttac
    14281 atattttgct ttggttccgc aaaatagttc ttgcagtttt tcaacatctt ttttcgtctt
    14341 ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt ttagcatctg ctgcctgtat
    14401 acctgattta tgagaaccag ccattggatg tccaccgaca aaccgaagat ttttctgctc
    14461 agcggatgtc acgacttgct gcttcgtact gctgacatcc gtaatgatga cgtgatcaga
    14521 caagttgatt ctttcaagga catcaagata agctaaggtt gtacggacgg gaacggctaa
    14581 taagataacg tctgcttgtt ctgcacctgt ttcaaaatca cttgcaacgt aatcaacgat
    14641 ctgctgttct actgcttgct cacgggtttt tgactgacta tcccaaccaa ctactatcag
    14701 ttctggattg gtctgacgaa tacaaagtgc catagaagca ccaatcagtc caagccccac
    14761 gataaaaaca tgttgtgcca tcttatttgc ccccttttta gtaatgtttc aagtagtcac
    14821 ggtactcctt cactgcagct ttcaactcaa caagcgaatc acttgggaat tttgacaata
    14881 gctctgtcgc taccacagtg gctacaacat tttccgcaac gacgctagct gcaggaactg
    14941 ctgtactatc cgaacgttct acacttgctt tgtaaggttc ttttgtatcg atatcaacac
    15001 tttgaagtgg cttgtacaat gtaggaatgg gtttcatgac cccgcgaatg acaagtggtt
    15061 cgccattggt cattccgcct tcgaagccgc ctagattatt ggtacgtcgg aaatagcctc
    15121 tttcttcttc ccataggatc tcatccatca cttctgagcc tggtaatgaa ccagaagtga
    15181 atccgacccc aaattctacg cctttaaagg cattgatact aaccacagct tgtgcgattt
    15241 tggcatctag cttcgtgtcc cattgtacgt agcttcctaa tccagctgga acgcctccaa
    15301 cgagcacttc tacaattcct ccgatcgtat ctccattttt ctttgtctga tcgatcaaat
    15361 cttttattct ttgctcaact gctggatcaa caactcgtac ttctgacgct tcagaaattt
    15421 ctttgatttc ttgaatcgag atatttccag gtatttctgc ttggattcca ccaagacaaa
    15481 tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat tttcttggca attgcgccga
    15541 ttgctacacg catggtcgtc tctcttgcag aggaccgttc aagcacattc cgaagatctc
    15601 gatgatggta tttcataccg cctaccaaat ccgcgtgtcc tggacgcgga cgtgcaactc
    15661 gccgcagtga ttggtctttt tcaggcactt ctgctacaga catgacagtt gtccaatttt
    15721 tccaatcttt attttctacc actaaagtga caggtgaacc aattgttttg ccgtgacgga
    15781 tgccagacgt gatccgaaca cgatcttttt cgatcttcat ccttcctcct ctgccgtaac
    15841 cagcttgtct gcgagcgagt tcgtgattga tttcttcttc tgacagaggt aatcctgctg
    15901 gtagtccttc aataatggct gttaattctg gtccatggga ttctcccgca gtcatataac
    15961 gcatcttaca gtcctcctaa gtaatctttg aatgtctctg tgggaacagg aacgattttt
    16021 gcttgcccaa tcttttctag caagatgata ttcaattggc ttcctcttgc tttcttatca
    16081 tgggttatcg cgtggaataa ttcatctgga tgacttagat cgtacgtaat cggcagatgg
    16141 aatttttgaa gtatcctttc taaagcagct gtcgatccag ttggagacaa gccgattttt
    16201 tctgcatgag aagtgatggc taccattcca atagctactc cttctccatg actgactacc
    16261 ccatatcctg ctgtattttc aattgcatgg ccaattgtat gaccaaaatt taaaataagt
    16321 cgatttcctt ggtcaaagac atcttcttcc actacttttc gtttgatttc caacgtagca
    16381 gtaatgattg gaacagcatg ttccagaaga tctttttcgt ctttcagctc gttcagctgt
    16441 ttccaaagcg ttgcatctgc tatagctgct gatttgatga tttcagcgat tccttctcgt
    16501 aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa tcaatacacc agctggctgg
    16561 ctaaatgttc cgacaaggtt tttcgctttg tttgtattga ctgctgtctt cccgccaata
    16621 ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa cgaaatggat cccacgcatg
    16681 aacgtcgagg ccacgaatgc tgctagatca cccaccattc ctccaccaag tgcaagtatc
    16741 ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt ataactcagc tgcttgttct
    16801 aaagatttac tggtctcacc cgccggtacg attgaaagga ctggttcaaa attagcttgt
    16861 ctcaattgga acatgatttt ttctgcatat agtggagcaa cgtttgtatc agtgatcaca
    16921 gctacttttt gaggtctcca aatcgtttga agccatgatc cagcctgttc tagtgcgcct
    16981 ttttcaatat gaatatcgta tttttggtta tcaacatgta cagtaagcat tcgtttcact
    17041 tctttctttt aaaaagctgg aattaatcga gtgtctgaat cttttccatt gcttctttca
    17101 tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc attcaacgat tggggaccgt
    17161 ctgaccaagc atttgcagga tcaggatgga tctctacgat catgccatct gctccgcttg
    17221 ccacacccgc acgagccatt ggtggtacaa gttcccaaat tcctgtacca tgacttggat
    17281 caacaatcac agggaaatga gttaattttt tcatcaatgg gacagcactt aaatcaaacg
    17341 tattacgtgt cgctgtttcg taagtccgaa tcccacgttc gataaaaatg acaggagatt
    17401 tgtcttcgac tgcaatgtat tctgcagcat taagccattc attgatcgta ccggaaattc
    17461 cacgtttcaa tccaacaggt ttacctgttt taccgacagc agatagcagt ttaaaatttt
    17521 gcatattacg tgcgccaatc tgtaggatat cgctgtactc agccaccata tcgatatgcc
    17581 cttcatccat cacttcagta atcactttca ttccaaattc atcagccgct tgacggatgt
    17641 atttcaatcc ttcttcttct aaaccttgga aagcatatgg agaagtacgt ggtttgaagg
    17701 caccacctcg cagaattttt gcgcccccgg ctttggccat tcgcgcacat tctctgattt
    17761 ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac gaaactgccg tcaccaattt
    17821 ttactccgtc tacatccacg actgtattgc caggatggaa ttctcgagaa gtcaatttgt
    17881 atgtgtttaa aatacgcaca gcattttcta ctccttcata gcgtgtaaat gctccttctt
    17941 gtaaacttcg cgtatcacct tttagtccaa tcactactcg ttctgttcct tgatcgattg
    18001 agactttgaa tccttctttt tcgatatgat cgacgacttt ttttatttgt tcttccgttg
    18061 cttctttttt cataattact atcattgttt ttcccgcttt cttttgttta cttatctatc
    18121 ttggctgatt ttctctactt tttcaaggtg gttttacatt aattcctact aaatgaccgt
    18181 gtcctgttgt tctcattttc gctccttctt tcctacttat gacccagata atcaacaaaa
    18241 aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc ttcaacgagc cttaaaaagc
    18301 gctgcaaaaa tataaaatag ccactataga tgaatttctc tacgtggctg taagtcgtgt
    18361 ttaaaaatct ttagccatca tagatacaaa cctggtttgt ttgtacccac gaagtcatga
    18421 atacaaacct atctaaaata gctaaagtaa tacgtattca gttttgtaag ttttctagat
    18481 ttcataaaaa atcgctccaa actgatgt
//
LOCUS       DAYYHS010000060        18504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000060 DAYYHS010000000
VERSION     DAYYHS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.060"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHA6A_001981"
     CDS             99..746
                     /locus_tag="ACHA6A_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8970151.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHA6A_001982"
     CDS             1139..3784
                     /locus_tag="ACHA6A_001982"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8970152.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHA6A_001983"
     CDS             4094..5407
                     /locus_tag="ACHA6A_001983"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8970153.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHA6A_001984"
     CDS             5397..6050
                     /locus_tag="ACHA6A_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8970154.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHA6A_001985"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHA6A_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8970155.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHA6A_001986"
     CDS             6947..7147
                     /locus_tag="ACHA6A_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8970156.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACHA6A_001987"
     CDS             complement(7401..9080)
                     /locus_tag="ACHA6A_001987"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8970157.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACHA6A_001988"
     CDS             complement(9082..9294)
                     /locus_tag="ACHA6A_001988"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8970158.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACHA6A_001989"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACHA6A_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8970159.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACHA6A_001990"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACHA6A_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8970160.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACHA6A_001991"
     CDS             13269..13466
                     /locus_tag="ACHA6A_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970161.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACHA6A_001992"
     CDS             13619..14104
                     /locus_tag="ACHA6A_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8970162.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHA6A_001993"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACHA6A_001993"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8970163.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACHA6A_001994"
     CDS             14981..15508
                     /locus_tag="ACHA6A_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970164.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACHA6A_001995"
     CDS             15599..16336
                     /locus_tag="ACHA6A_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8970165.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACHA6A_001996"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACHA6A_001996"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8970166.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACHA6A_001997"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACHA6A_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8970167.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5983 a         3199 c         4022 g         5300 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccataagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAYYHS010000061        18113 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000061 DAYYHS010000000
VERSION     DAYYHS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.061"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..976)
                     /locus_tag="ACHA6A_001998"
     CDS             complement(<1..976)
                     /locus_tag="ACHA6A_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8970168.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIREST"
     gene            complement(1000..2484)
                     /gene="galT"
                     /locus_tag="ACHA6A_001999"
     CDS             complement(1000..2484)
                     /gene="galT"
                     /locus_tag="ACHA6A_001999"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8970169.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2496..3485)
                     /gene="galE"
                     /locus_tag="ACHA6A_002000"
     CDS             complement(2496..3485)
                     /gene="galE"
                     /locus_tag="ACHA6A_002000"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8970170.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            3734..3901
                     /locus_tag="ACHA6A_002001"
     CDS             3734..3901
                     /locus_tag="ACHA6A_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8970171.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(3963..5774)
                     /locus_tag="ACHA6A_002002"
     CDS             complement(3963..5774)
                     /locus_tag="ACHA6A_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8970172.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(5771..6136)
                     /locus_tag="ACHA6A_002003"
     CDS             complement(5771..6136)
                     /locus_tag="ACHA6A_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8970173.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(6299..6694)
                     /locus_tag="ACHA6A_002004"
     CDS             complement(6299..6694)
                     /locus_tag="ACHA6A_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8970174.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(6712..7674)
                     /locus_tag="ACHA6A_002005"
     CDS             complement(6712..7674)
                     /locus_tag="ACHA6A_002005"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8970175.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(7674..7886)
                     /locus_tag="ACHA6A_002006"
     CDS             complement(7674..7886)
                     /locus_tag="ACHA6A_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8970176.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(7907..8605)
                     /locus_tag="ACHA6A_002007"
     CDS             complement(7907..8605)
                     /locus_tag="ACHA6A_002007"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8970177.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(8625..9167)
                     /locus_tag="ACHA6A_002008"
     CDS             complement(8625..9167)
                     /locus_tag="ACHA6A_002008"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8970178.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(9299..10303)
                     /locus_tag="ACHA6A_002009"
     CDS             complement(9299..10303)
                     /locus_tag="ACHA6A_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8970179.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(10300..11289)
                     /locus_tag="ACHA6A_002010"
     CDS             complement(10300..11289)
                     /locus_tag="ACHA6A_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8970180.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(11286..12092)
                     /locus_tag="ACHA6A_002011"
     CDS             complement(11286..12092)
                     /locus_tag="ACHA6A_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970181.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12258..13214
                     /locus_tag="ACHA6A_002012"
     CDS             12258..13214
                     /locus_tag="ACHA6A_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8970182.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(13291..13809)
                     /locus_tag="ACHA6A_002013"
     CDS             complement(13291..13809)
                     /locus_tag="ACHA6A_002013"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8970183.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(13897..14046)
                     /gene="rpmG"
                     /locus_tag="ACHA6A_002014"
     CDS             complement(13897..14046)
                     /gene="rpmG"
                     /locus_tag="ACHA6A_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8970184.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            14274..14720
                     /locus_tag="ACHA6A_002015"
     CDS             14274..14720
                     /locus_tag="ACHA6A_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8970185.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(14915..16810)
                     /gene="asnB"
                     /locus_tag="ACHA6A_002016"
     CDS             complement(14915..16810)
                     /gene="asnB"
                     /locus_tag="ACHA6A_002016"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8970186.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(17135..18109)
                     /gene="bsh"
                     /locus_tag="ACHA6A_002017"
     CDS             complement(17135..18109)
                     /gene="bsh"
                     /locus_tag="ACHA6A_002017"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8970187.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         5480 a         3823 c         3108 g         5702 t
ORIGIN      
        1 tcgttgattc tcgaataatc aactctgttt ccacaataat ctttcttgaa accggtgctt
       61 tggaattact taattgaaga atcgtctcca ctgctagctc tcccatccat tcggtataaa
      121 cttttacagt agaaagagag ggcgttacat atttcgctac actgacatca ttgaaaccca
      181 tcacagaaat atcttctggt accactaaac ctttttcttg aatcgctttc agtgcgccaa
      241 tcgctaatgc atcattagct gcaaaaagcg catcaggaac atgcggattc gtttttaaat
      301 actgcttcac tgcttgatat cctgactcga cagaaaaatc tgcttcgata attggacctt
      361 cgtcgtagcc tttttctgcc aaaacttgtt gaaatgccat aattctggga tcttttagcc
      421 gctgatggtt tttcttcgta tattctgttc cagcaagcat agcgatatgt tgcgctttat
      481 gggagacaaa atgttcaatg actttttgaa cacttgactt aaagtcgaca actagagagt
      541 cgattccttg agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg gatcctttca
      601 tttctaataa ctgagcttcg tcgaattttc ctagtgcgat cgttccatct gtcggaagat
      661 cactaatgga atcccacgtt tctttcaata aagtaacgtt tgcttcttct gccttttttt
      721 cgattcctag acgaatagat aaataataaa gatcttcaag ctcttccgta tcgtcgtacc
      781 actggatcag gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt ttcgtataat
      841 ttaagcgttc tgctgcttca aaaatttttt gctttgtggc taagccaacg gaaagatcag
      901 gatcatagtt gagaactctt gaaacagtag caggagaaac tcctgccaac tgcgcaatgt
      961 ctttaattgt tgccatttgt ctctcctcct tttttacgtt tataaaattg cagtaaatcg
     1021 ttcaaacgct gcctgtcctt tttcatctcg cttgaatact ccagcgtctt ccagtacacg
     1081 tgcaaaaacc tgacctacag atttttgtac gacatcggtg accgtatctt tcgtcaaatc
     1141 aggatgttct ttttttaatt gttctgccca cggacgatga tattcagcca tctggttttc
     1201 ttctcctaat aagaaatgct ctacttctac tagttctggt ttcaaacgag gtggtaaaac
     1261 agcaagcccc attacttcga tcaacccaat gttctccttt ttgatatgtt ggacatcttt
     1321 atgtggatga aaaatcccat caggatgttc ttctgaaaca ttgttgtctc gaaggacaag
     1381 gtccaactca aagtaattgt cccttcttcg tgcaattggc gtaatcgtat ggtgtggtgt
     1441 accatctgct gaaaaagctc tgatagatac tgtttcatcc gaatatgctt tccacttttc
     1501 caatactgag ttagctgctt caatcaacaa ttttttctct gttgattgca aacggatcac
     1561 agacatcggc catttcacga ctcctgcgat gacttgcgga taatctgcta gtttgataag
     1621 tttttctatc ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat aatgatcatg
     1681 agaaagaatc gatcctccaa caatcggcaa gtcagcattc gaaccaacaa agtaatgagg
     1741 caagacttca gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt tcatcgggcg
     1801 gtgttgttct gataaaatga tcgaatgttc attgtaataa gcataaggag aatattggaa
     1861 tccccagctt tcaccgtcta gattcattcg aataatccgg tgattggtgc gggctggata
     1921 gttcaagcga cctctatatc cttcgttttc catgcaaagc atgcaggtag gatagtctac
     1981 ttttaccgcc tcgcgttctg ctgcgatttg ttttggatct ttttctggct tagataaatt
     2041 gattgtgatt tccagttcac catactccga ctctgccgga aatttgatgt tttttgcgat
     2101 ggctcttgtt ttgatgtagt catttttctt acttaaatta aagaaatact cagttgcttc
     2161 ctctgggctt ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag gtggcggtgt
     2221 gagaaaatcc attaattgcg cttcaaaaat ctcttgttct gctggcaatt ctgaaagaac
     2281 accattttct cttgccttag cgacaagctg atccaataag tctacagatt cttccttaac
     2341 tggtcttact tcaatgcttt ctagagcatc ttctcctacc atccctaaaa gacgattctg
     2401 taaatataag cggtccattt ccatccatcc gccagcttcg atcgcaagtg tggcaaaatc
     2461 acaaatcgtt tgactgattg acatatctca ttcccctatt cttcataacc atttggatgg
     2521 ctgacatgcc aatcccatgc tgttttgata attgctttga tatctgtgta ttcaggtttc
     2581 caacccagaa cctctctcgc tttttcactt gaagcgacta atcggctagg atctccagct
     2641 cttcttggtg ctatttctgc tggaatttct tttcctgtca cttctctagc tgcttctagc
     2701 atttccttga ctgagtatcc tttgctgctg cctaaattga agaagttgct ttcatttcct
     2761 tctttcaagt attccaaagc taaaatatgt gcagcaatca aatcttctac ttgaacataa
     2821 tctctgatac atgttccatc tggtgtatca taatcgtctc cataaacagc caatgctttt
     2881 ctttgaccaa gagctacttg taaaatgatt gggacaagat gcgtttctgg tgtatgatct
     2941 tctccgatag atgcatctgc ttttgctccg gctacattga agtagcgcaa agcgacatag
     3001 cgcatgccat atgcttggtc acaccacttc atcatttttt ccatcatcaa tttactttca
     3061 ccatacggat ttttaggatt agctggtgta tcttctgtga ttggagactc ttttggttct
     3121 ccgtaagttg ctgcagtaga agagaacacg attttattca cattgaactc gtgcatgact
     3181 tcaagtaaga tttgcatacc gtatacgttg ttgttgaaat acatcaatgg tttttcgaca
     3241 gattctccta ctaaagagct tgcagcaaaa tggattactc cttcaatcgg ttccttttca
     3301 aatacggaac gtagaaattc tttatcccgg atgtctcctt cataaaaatg agcatctgga
     3361 tgtactgctt gtttgtgacc tgtgagcaaa ttatctacca cagctacttg ataccccttt
     3421 tgaactagct ggtctacagc gtgcgaaccg atataaccgg ctccgcctag tactaaaatt
     3481 gtcataaaaa acactccttt gattagtaaa attgtgtttg ttgattatat ataatttaaa
     3541 aaaacgaagt ttacttttat ggtgaaaaca aacaaagtaa aatttcgtaa attcatatgg
     3601 taaatttata ataaaacatt tagtaaacat tttcaaggat tttttgcaaa ggtttacaaa
     3661 caaacgtaaa actagccttt ttttctcttt tgttccataa tagatataac aaataataaa
     3721 tgaggtggat tcaatgaaaa aatttgtatc tggaatatta gtaggaagtc tggcaacagc
     3781 cgctgccgtt gctggtttgg tcgcttctgt gaaaaaaaca gtgatcgatc caattgacga
     3841 aaaagaagca atgatcgaag aaaaccgtaa aaaagcaatg cgcaaacgcg tttctcgtta
     3901 ataattattt aaaaacacgc atgaagactt gtgaaagaac agataagtca tcatgcgtat
     3961 ttttatttgt cagtcaatat aagttgtgct attttatctg cctgtctcag attccatgga
     4021 tctgtataag gtctagctac tgctgcagtt agccaatcca caccttcaac aggtattcca
     4081 tagcaataaa agtcagactg gatttcccct gaagcatcaa tcacgcgatt cgtacctcgc
     4141 tcaacatcta tagcttctga atgataactt cctgtattat ctgaaaatga aaaagggcga
     4201 acaagtttgt tttccttcaa attttttatg acaggattac ttgaacgaga aaagcttgtt
     4261 tgtggcaatc gagcttcaat caaatagcga ctgctgattt ctttatcttg tagagaaata
     4321 acatagcggt tgtgcttttg ctgaatatca aatggcggtt cgaacaaatg aaaaataccc
     4381 gcatctatca gtgctgctaa ttctttcgtc cgaatgactg gcggaccgat cgttaaaaag
     4441 ctatttagat gagtaaacca tccccatagt atttctttgt attcctttgc agaaaaaacg
     4501 tgccattcaa tagcctgccg gattggatca cgccagtctt ttaatgcatc aaatgttgaa
     4561 gcaaatgctc ctgtgcaatt gcctttttcc gcttctttta tctgcccttc aataaatgct
     4621 cggctagctt ctgcaaagga ctcatcctga taggtaagag gaatttctaa atacgaccaa
     4681 tcccatctat aagggattag tgctggatgc tttccgagac actcctctga agagagggag
     4741 agaaaatcat gctcaaaatt tttctttgga atcggcaaat gatattcttc gatgattttc
     4801 ttgtaataaa acaactcaac gtcttttttc agatagtcaa aaaatgtttc gccagtcaaa
     4861 aatcccgact gatgccattg ctccaggttt tcttttgtta gtaaccgtgg ccatgccatt
     4921 gatccgcctt gcttttggtt tcttcctcga gggtaataag ggagccccct tcttgatcca
     4981 ctgtatacaa tcggttcctt gcctgatgga tgatagacaa gttttccttt ttcgttctcg
     5041 aagcgccctt ctcttgatac tgtaaacaat ccgacataat caaaaaaact taagcctaga
     5101 cctcttaaaa agacattttc tttaggcggt atcttcgaaa gatcgacatc tgcaggatta
     5161 gaaggaagct gatacagtaa atcatttttc tttgcgtagt ctacccacac tctttcttct
     5221 tctgtctcat cattttccca gtgaccgctt gctaaaacaa gctgatcgat agaatagctt
     5281 tgctgatctg tctgtaaaag ataaccatct tttcttttgc tgactttttt tactaaagaa
     5341 taaacaaact gaatagaagc tggaaattct tcttgcaact ttgagaaaaa ccagcgttga
     5401 tagagtccgt ataaacatcg agtagattgc tgatcttttt ccagacgatt gatttcttct
     5461 ataattagtt gcttattttt ggttaccgtc tgttcgatat aatctgtagc aggtccttta
     5521 ctccattgaa acaagttggg tccctgtgtt accaatcctc cactggacaa tgtttcatct
     5581 gtaaaaagcg tcacttgttg agaaacagaa ttcattaata actccggtgg ctgattcaac
     5641 cgccaaactc gacctccagg accattggga tcgaaaagaa aaagttcaag agaatgtctg
     5701 tcctgattat tatggagaat cctttctgct atacttaatc ctctaggtcc ggcaccaatg
     5761 atccctattt tcatttgcat gcctccattt cgataaattt tttcaataaa acagcatggt
     5821 tctgatgttc attttttgca ccataaacaa aagtaatgac tggatgttct ttcataagtt
     5881 gactgagttt aaaaatagct tgcttctttt cttcttcgaa aatcaattct tgctggtatt
     5941 tctttgcaaa tgaagcaaat cgttctggta catggtcaaa agttttgcgt gcttcattac
     6001 taggtgcaat ttctttcaac cacagatcta actgcgcttt ttcttttgaa attcctcgtg
     6061 gccagaacct atccacgagt attctatagc catcctcttt ttcagcatca aggtaaacac
     6121 gttttaattt gatcatagtt gtttccttct ttctgccttt tcgcttagct tatatatgta
     6181 gataatagca caaaataaaa aaaatgaacg gcacaactat ccattgcgcc gttcatgtga
     6241 tcttggtcaa aatctaatga ttgattcggt ttttaaagct gtcatacaat catctgtttt
     6301 atactttttt acttttgatt tttccagacc atccgtcata tccaccctta agtaaataaa
     6361 tctcttgata acctgcttta cgtaatttat ttgctgtacg gatgctcaag cttttctttt
     6421 gatcgtaaat atagacaggc tggtcttttc gaatagatcc taatgaatct ttcaaaacag
     6481 tgtaagggat gtttcttgca cctaaaatat gcccagcatc aaacacatct ttttcgcgaa
     6541 catcgattac ttgtgctttt ctcattcctt ctcgaaattc ttcttctgtc aaagtcgttg
     6601 ctgaacgctt ggccattact cgtagataga cttcccaaaa gatgattgca agcacaatca
     6661 atagcaaaat gatattaatt acccataaaa ccatgtaatg cctccgtttt tttaagcaaa
     6721 tttcaaggct aatgaggcag caccaatgac accagcttca ttaccaagct ctgctaattt
     6781 gatttttgtt gattctctta cttgcgggaa agtaaattct ttaaaatatt tctctacacg
     6841 gctacgtaaa aattctcctg ctgccgatac tcctccacct aacacaatac ttgacggatt
     6901 caatgtattc cccaagttgg cgcaagctaa acctaagtag aagcatacgc gatcaacaac
     6961 catcaatgca aacggatcgt cttcttgagc catttcaaag atatctttac ttgttacttc
     7021 ttcaccgttg tctaacattg ctttcagttt tgaatctcct gcgtattctt ctgccaagta
     7081 acgacctaga cgtaccacac cagtagcgct agacacagtt tctaaacatc cgcgtttacc
     7141 gcaagtacat tcgaaccctt caggatccac agtgatatgg ccgatctctc cgcctgctcc
     7201 gctcacgccg tgaatcaaat ttccgccagc aacgatacca ccgccaacac ctgttcctag
     7261 agtaacgaat acaacgtctg gatcgttttc tccagcacct ttccatcgtt cacctaaagc
     7321 agcaacattc gcatcattat cgatagcgaa tttgattcct gttcctgcct cgatcagttt
     7381 tttcacaaat tgaacttctg tccagtttaa gttatacgca ccaataaccg ttccagcttc
     7441 gctgtccacg cttcctgggg tacccatgcc gattccgata aagtcttcgg ctttcatatt
     7501 atataaattc aaacgatggt tgattgattc aatgatttca ggaacgatat gcttaccatc
     7561 atccaaaata ttggtatcga tgctccattt ttgttgaatc tctccttctg gtgttaaaat
     7621 tgcaaatttt gctgttgttc cacctaaatc aatcccaata atcttcttgt ccattacaaa
     7681 tttcctttct ttcggctttc ttcaattcgg tgctctcttt ttaatatgcg ccacgctgtc
     7741 atgtatgtat catgatcaat caaacggcta tcatacagat tacgcaattc gatagtcatt
     7801 aattcaatgt catagatgcg tgcacccaca tagataaaga tgccaaattt tttgaacagc
     7861 tgctgtacat catataagct ctccatcttt aagcacttcc ttcatattat acaagtaaac
     7921 catatttttt taaacccagt aaaatacata ttacaaaaag aaatacaaaa actagtgtag
     7981 aaagaatcct ttggtgtatc gaatagcttg tctgttgttt cggtagaccc aaaacattcc
     8041 ctaacactaa tccgccaatt agcccaccga catgtcccca aacatctaca gaggtatcca
     8101 gaagaccaaa aacaaatgtc aacacaataa ataaaagaaa atgtctgact agctgctgga
     8161 tcgctgggtt atgtttaaaa tgaaacccta agataaacaa cgcaccgaac aaaccgaata
     8221 tcgaagtact cgcaccacca gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat
     8281 ttcccattat cccgctgaac atataaatca agaaaaaacg ccaatggcca tagattgcct
     8341 cgatttgctg ccccataaaa tacagtatca gtgagttcat tacgaaatgc atcagtccaa
     8401 aatggatgaa gattggtgtc actaaacgcc accattcatt gaagtgaaca agataaggac
     8461 catacattcc ggcataataa gggatctcaa gtcgcggaaa taattccatc atgaggaaga
     8521 ccgctgtctg tatccctaaa aaagcatagg tccataacgg tcgtcttaac catgcacgca
     8581 attttatttg ctgttgataa ttcatgaaaa cctcccattt cattttagtc aataaacaat
     8641 tgttggatct tctgatcaaa gctctccggt tgccattgtt cattcaattg ttcacgaaaa
     8701 acaaggctac atgttttttt atgaaaatta tttaaatacc ggtcgtaata accaccgcca
     8761 aaaccgattc gataaccggc tctattgtag ataatacctg gtacaatgag gagatcgatc
     8821 atctccggca aatagagctt ctctgattgt ggctcaagaa caccaaaact cgaacgtaca
     8881 agaacggact gttcatccat ttcataaaac gccatctgac gtttcggcaa agttttaggt
     8941 acaactattt tcttttgttc tttttctgct cgttggataa ccggctgtgt atccagttca
     9001 aattcattcg ataaagtcgt ccctatcact tttgctgact tccagtactc gctggcgaat
     9061 agctgtttat agattcgctg ttctttttcc tttttcttgc cacttttggc cagatttttc
     9121 agcttttcga ttcctttccg gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc
     9181 cttctctcat aaaatggtat aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat
     9241 gcataaatag aataaaaaga agacctagaa atgattggcc ttcctttttt aataaatttc
     9301 aactgttcag cgcgtgtttt tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa
     9361 aaagggcaat caaaattcct gtaggtactt ccccacttgg tagaatcact ctagcaacca
     9421 tatctgcact aagcagaaaa atggaaccga acaaaccgct cagaacaaag gtccagttat
     9481 tttctttacg tacgactagc ttcgcagcat gaggagctag tagtcctaca aaagataaac
     9541 tacctgtcac cgctacacta ccgcttgcca aagcaacagc taacaacaaa aggatttttt
     9601 gacttttagc cacgttaagg cctaatcctt gcgcgcgttc ttgaccaaat gatagcaatc
     9661 caatcctctg tgcataaagt cctcctagcg gaattaagat aaccgcccat ggaagcaatg
     9721 cccaaacata aggccagctc gcaccccaga ttgatcccgc taaccatgca ttcacaaatc
     9781 cataattttc cttagataat tggatcgtgc atagcaatgt taatgcagaa aggccggcat
     9841 taacggcaat accggacaat aagattcgat tcatacccaa aaagccattt cgatcatagg
     9901 ccgcaagata gacacagcaa gcagctaaaa caccacctag aaaggctaca actagcaata
     9961 gaaacggcag cgaatagtgc gaaaagaaac ctaaataaag catcactaaa aatcctgctc
    10021 cggcattaat gcccaatata ctagaatctg ccaattcatt tcttgttact ccttgcaaga
    10081 tataaccaga aacgccaaga caagctcctg ctaataaact gatcagtaat cgaggaactc
    10141 gaaattctct aacaatcagc gccaaagaag atgattcttt ccctatgaac gtggcaaaaa
    10201 gctgatccag gtaaataaaa ggcgtaccaa tcattaaact acctatagac aggatgacaa
    10261 gtacaaaaag caagaaatag aacgtccaat atattttttt caccgttgtt ccctccttac
    10321 ttgtatcagt aaaaacggaa cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa
    10381 agggggattg atcatcctcg ctgtaaaatc cgccaacata accaatccac cgccaactaa
    10441 aaaagagaca ggtagaacat aacgatagtc cgttccaacc attccgcgaa caactggcgg
    10501 aaccaacagc ccaataaatg aaacaggtcc gactaacgaa acggcaatgg ctgctaacac
    10561 tgcaactaga cagaaaacaa tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc
    10621 atgtgtttca ctcaaatgaa tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac
    10681 agcaagtata tataaaggaa acaatactgc taattgtttc catgtgatat tggcagctcc
    10741 gcctacaaac caaaaagcaa gatcctgctg cagattaaag aattgggtaa gtgcttggct
    10801 gatagcagta aaaaaagaac taatagctac gccagctaaa atcagctgta aaggattcat
    10861 gcccattttc gaaaactgca aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc
    10921 caatgacatt agaaacacag aaaaatcaga agctttaggc caaaaagcaa aactaagggc
    10981 cattgccaac gaagcacccg cgttgattcc tagtaagcct gaatctgcta aaggatttct
    11041 tgtcactcct tgcatcaatg caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc
    11101 cgccacacga ggcaaacgaa tatttcgtat gatttggtga ctttgttttg atctatcaaa
    11161 atcaaacaac gcctgccaaa aatctgcttt tcccacagtc gctgcgccat ttgaaagagc
    11221 aacaaaaaaa ataatcaaaa gaacaaaaca gaccaaaagc aggagtctat ttttcttcat
    11281 agtcctcata attgatcctc ctgctctttt ggcaacaaat cataggttag aaaaactggt
    11341 ttatttgttc ttggaagcgt gacaatcgtt gcatcaatct caaatatttt tttcatacac
    11401 tcttttgtga tcacttcttc aggtgtacct tctgtgatca acttaccgtt tttcattgct
    11461 aataaataat ctgaaaaacg tgaagcatga ttaatgtcat gaatcgacat caagatcgtt
    11521 gtttgtttca tttgattgat ttcagtcaat aactgtagga tctctaattg gtgtgccgga
    11581 tctaaaaatg ttgtcggttc atccaagatc aagatatctg tttcctgagc aagcgccata
    11641 gcgatccaga cacgctgggt ttgtccacca gacagcgttg cgatatcccg catacgaaat
    11701 ccagctagac ctgtagcatc caacgcccac tcgattgctt catgatcttg cgtgtccatg
    11761 ccactgaaca gtttttgata agggtatcgt ccgtaagata ctacttcttc tactgtcata
    11821 cctgtagggt gttcactagt ttgtgctaat aatgtaattt tttttgcaag ttcttttgaa
    11881 ctgtatgtag agatatcaga gttattgaca atgatctttc ctgatttcac aggtagtata
    11941 cgggtaaaac ttttcaataa agtagatttt ccacttccat ttggcccaat caaactagtg
    12001 attttccctt caggaatcgt aacagacatg ttttcaatga tttttttgtt ttcgtagccg
    12061 gctgcaatgt cagctgctct taatctcgtc atttcaccca tcctttcgct gaaaatcatt
    12121 ctcaattatg acgctgtttc aacatttcgt caagcgctac tttcatatct atagccaaat
    12181 gaagcggttg acagcgttcg aaatatcagg aataataacc aatgagaaag attctcaatt
    12241 atgttaggag gaaagaaatg aaattaaaaa caaaagtatt cacattatct agcatattgc
    12301 tgctggctgg agtactgagc gcttgctccc cctcttcaga taaagaaaca gctacaactg
    12361 ccaattcgac gacttcatcc aatagtactc atacagtcac agatacactt ggacataaag
    12421 tagagattcc aaacgcgccc aaacggatca tcggcagtta cttagaagac tatctgattg
    12481 cactagggga aaaacctatc gcccagtgga ctgttggcag tggttccatt caagattatc
    12541 tgcaagatga tttaaaggat gtcccaacga tttcttatga ccttccttat gagaaagtgt
    12601 taagctttga gcctgatttg ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat
    12661 acgaacaata tagcaaaatc gcgccaactt atgttgtaaa aaacggaaca gatgtcacat
    12721 gggaagaaca actaaaagat gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaaa
    12781 tcatttccga ttatcaaaaa tccatcaaga aaactcgaga agaactagca gataaaatcg
    12841 agaacaagac agctgcagtg ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa
    12901 atcggtcaag tggaagaatc gtatacgacg atctggaatt tggtgttcct gatctagtgg
    12961 aagaggtctc caaaaaagcc acttctgact ggtctgctgt ttcctctgaa aagctagctg
    13021 aattagatgc tgactacatc atccttgtga acagtgacaa agatgctgcc atgttcaatg
    13081 aaccaagctg gaagaatctt aaagcagtga cagacaatca tgtactgcaa ttcggacctg
    13141 aatctagttg gctatataac ggacctattg ccagtcagga aatggtcaat gatatcaaag
    13201 aaaaactaaa ttgattttaa ctaaaaaaag aatcttcacg attagtcgtg agatgctttt
    13261 tttcttatta acaaactaga cgcatagatt ttattcttct tttgaagcaa ccctattttt
    13321 tactctttcc catttttgaa aacgatgggc atatagattt tcttgatccg ttatcccctc
    13381 atactcctct ataagggcga agtctgaaaa atcaatattc ccaatatacg tatcgccttc
    13441 aaacgtgcta tcgatcaatg ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa
    13501 aatccgacta ccacccgaaa catagatatc ttctgagatc gtttgactat acgacaaaag
    13561 ttcatcgatc gaatgcatta cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt
    13621 caacacgatg atatggcgat aaggaagtga aagatccccc attccttcat aggtctttct
    13681 tcccatcact aaaattcgat ccattgtatg ttcgcgaaag aaacgcatat catttggcaa
    13741 tcgccatggt aacaaaccat ttttgccgat cagaccattt ttatcttgtg cccacatgga
    13801 aataaacata ttatccgctc cttatgattt tagatgtttt gagataaaaa aagtctcaat
    13861 tctttgacca gcaaggaatt gagaacttta aaaacattat tttgtttcac ggtgcaaagt
    13921 aactttgcgt tcgcgtgggc aatatttttg tttttccaaa cgatcaggat tgttacgttt
    13981 gtttttgctt gttagatagt tacgttcttt acaagaagta cactctaaag tgatgtttac
    14041 gcgcatgttt ttccctccca tttttacaag aatcctctaa gatgactctc agacttgatt
    14101 atcttaccat gattttttct agattgctag tgtttttcac gattttgtta agtaaaacta
    14161 cttgacgata aatacaacgg ttttacttct caaacaaaat ctttttcttt ttgtataaat
    14221 acccttacac ttgtttatat agattatgtt ttttaataaa gggggatact aatatgccat
    14281 ggaacatgaa ggactttcct gcatcaatga aaaacttaga taaattgaca agaaaaaaag
    14341 caatcgacat cgcaaatgca ttattagatg aagggtaccc tgataaccgt gcgatcccaa
    14401 ttgccattga tcaagcaaaa aaatggtatg aagatgctag tgaatcagaa aaacgaactt
    14461 tcgaaaaaga aaaaaatcca agcaagacgg atgaacacga tacgaatcct cgtgctggaa
    14521 aactgcttga ttcagatgtg atcgtagaat atgaggaaga acaatggatc gtgaaatcta
    14581 aaggggcaaa aaaggcaagc aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa
    14641 agcaagtagc acaaaacaaa gaatcaactt tgttcattta taaaaaagat gggacaaaag
    14701 aaaaagaaat cgattattga acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc
    14761 cccttatgtt cgaaacaatg gtgttcctct gccaactccg aaccataagt cataatatct
    14821 ataaaacggg aagagctgtt ttttgaaatt gtttcttatc actcagtgtt aaacggtagg
    14881 agaaaacctc ttttttattg atatttgatt ttcatcaaac agtcacatag tcgattgctt
    14941 ctgcttgcgg aatcgtttca tctacaaagt agactttata ccatgtcaag aaactgaaga
    15001 ttgtccagat ttttctttgc tcttctttac gtccttcgtg atggtcatct agcaacttca
    15061 tgattttttc ttgatcaaaa aattcttttg caaaatctgc ttcgaataat gctttgactt
    15121 gttcgtagcc gtgttcttct ttcaaccaag ctttgattgg cacagggaat cctaatttca
    15181 cacgattcga ccattcttcc ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg
    15241 tgttgttttg attcaataaa aacttacttg gtatttgttg agccaatttc atgacttcga
    15301 tatccaataa aggtactcgg acttccaaag aacttgccat agaaagttta tctgccttta
    15361 acaagatatc ttttggcatc cattgatgaa gatcgacata ttgcatctta ttgacttcat
    15421 ctttgattcc ttctgttttc tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg
    15481 tttgatattt tggtgttaac acttctgatg cttctgattc ttcgaacact cgagcatgac
    15541 cgataaaata ttcttttgca ggagcaagtg attcatataa atggatccgt ccatggaaat
    15601 ttttcatttt tccgatttta cgtcctaaat tatagcgtgt tccttttggt aattttttca
    15661 atccttgagc aatcacacgg atgaattttg aattcgtatg gaatccatac gtttggtatc
    15721 ccgcaaacag ttcatccgcc ccttctccag attggactac ccgcacactt tgtgaagcaa
    15781 gatttgctaa aaagtacaaa ggtacgcaag aaggatttga atcaggttca tctaagtgat
    15841 actggatcaa agggaatgct ttgaacgaca tgtctccgtc gatcactgct gctgtattat
    15901 ctaagtccaa tagttcggtt agttttctgg cttcaatcgc ttcattatag gttttatcat
    15961 caaacccgat tgagaaagaa tgatctggtt tcaatacaga ggttacataa ctggaatcaa
    16021 ccccgctgga taagaaagaa ccaacttcta catcactgac cgtatgtgct tcaatagaag
    16081 cttgaacggt ttcgtcgatt ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt
    16141 caaaatctgc atcccaatat tgctgaatgt ctagttttcc atctttatac gtataataat
    16201 ggccttcagg caaacgatag acatctttga agaatgtttc gccgtttaat ggagaatatt
    16261 ggaaagtcat ataaggtttc aacgcttctt tgttcaattc tttgttaaaa tcaggatgag
    16321 gcagaaaact tttgatttct gaaccaaaca taaatgttcc gttcatttct gcataataat
    16381 atggtttgat cccaaagtgg tctcttgcac cgaataactc attcttttca ttatcccaaa
    16441 tagcaaaagc aaacattccg cggatctttt gcaataattc tgttccccat tcttcgtaac
    16501 catgcaaaag gacttctgta tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt
    16561 cttctctcaa aggctggtaa ttatagattt cgccattaaa aataatgatc tttgtccgat
    16621 cttcattgta aataggctga gtaccgccct ctaagtcaat aatacttaat cttctgaatc
    16681 ctaaagcaac atgtttatcg atatattctc ctgaactatt aggtccacgg tgaacgattc
    16741 gatccatcat tgtattaata attgtttttt tgttgtcctt gtcgttaaca aaacctacaa
    16801 taccgcacat ttaactttca catcctaata taaaattact gtttatttca ttcatatgca
    16861 tcttatcata gaataaatag cggacgaata ttttctctct tacaatttcg tcacgtgaaa
    16921 acaaaagatg aaagtttctg tttttacaga aaataaagtc ccaatatcta atttaccatg
    16981 ttttcttttt tgaggctacc ttattctctt cgtgttaaaa aaatattagt ggatgctatt
    17041 tccaaaaaaa acagcaaaaa aagacacgac ctaaagatcg tgtctaatta tgactgtatc
    17101 agaaaatatg ataccaaacg gatctattca ttaattaatt gacatagttt atctgttgct
    17161 cttttatgac tggataactg accaattcat gacgatccaa gtcttcttta tttatattta
    17221 cagcagtaat ttgactgtct tcatacgtac gataataata aatcccttta tcaacgttac
    17281 agcaagaaga ataaatcgta tattcgtatt ttccttctcc agtatcgcat actccttttt
    17341 gttgctcgac tgagcctagt atatgaaaaa attgactgat actttcagat tctgaatcac
    17401 ctgatgcagc attcatttta gtaaatgtgg cttttacaaa tcgagaaaca gaagagagat
    17461 ctccaggtaa gcccaatcct cccatcccac gactataaac attcaagctg atctggtttg
    17521 aaaaatgatt ttctggtgtt ccatttgata gcgagcgata attgttcaga ttaaataatt
    17581 gataatcaaa tggcggatta tttgtcaaca caccgacagg attatcatat aaatgaagtc
    17641 catctttcat actttcaatt actatcgatt tttctttatc agctaataac cagtgtaaag
    17701 gtgacaaggg tagtttttca ctaaaatcga tattgactag attgatagta gccaataatt
    17761 ctttagcttc gtctatggtt gagcattgcc ctagtatcca tggaataaac tcaaatggtg
    17821 caacgttatc ttttccttct tgtaattctt tataatcagc attgccagga aaattcagtc
    17881 ctgccatact caaccctttt tcatttgtag cctcataata gagtggatag ttggataccc
    17941 cagctgctat tcctatcatt gcataatgtt tatctaaatt ttttaccttt ctaaaatcga
    18001 aacgataatt tcttggagta atagtgacta cttcattata agacatctca taatcaaaat
    18061 tccttccaaa ataatggtct ttcgttgcat aagtgattgc ggtacacatc gtt
//
LOCUS       DAYYHS010000062        16874 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000062 DAYYHS010000000
VERSION     DAYYHS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.062"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..555)
                     /locus_tag="ACHA6A_002018"
     CDS             complement(43..555)
                     /locus_tag="ACHA6A_002018"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8970188.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(569..2866)
                     /gene="recJ"
                     /locus_tag="ACHA6A_002019"
     CDS             complement(569..2866)
                     /gene="recJ"
                     /locus_tag="ACHA6A_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8970189.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3008..3451)
                     /locus_tag="ACHA6A_002020"
     CDS             complement(3008..3451)
                     /locus_tag="ACHA6A_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8970190.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3452..4240)
                     /locus_tag="ACHA6A_002021"
     CDS             complement(3452..4240)
                     /locus_tag="ACHA6A_002021"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8970191.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4237..5178)
                     /gene="rnz"
                     /locus_tag="ACHA6A_002022"
     CDS             complement(4237..5178)
                     /gene="rnz"
                     /locus_tag="ACHA6A_002022"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8970192.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5355..5942)
                     /locus_tag="ACHA6A_002023"
     CDS             complement(5355..5942)
                     /locus_tag="ACHA6A_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8970193.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6163..7476)
                     /gene="obgE"
                     /locus_tag="ACHA6A_002024"
     CDS             complement(6163..7476)
                     /gene="obgE"
                     /locus_tag="ACHA6A_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8970194.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7764..8774
                     /gene="gap"
                     /locus_tag="ACHA6A_002025"
     CDS             7764..8774
                     /gene="gap"
                     /locus_tag="ACHA6A_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8970195.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8859..9794)
                     /locus_tag="ACHA6A_002026"
     CDS             complement(8859..9794)
                     /locus_tag="ACHA6A_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8970196.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            9980..10939
                     /locus_tag="ACHA6A_002027"
     CDS             9980..10939
                     /locus_tag="ACHA6A_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8970197.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11025..12119)
                     /locus_tag="ACHA6A_002028"
     CDS             complement(11025..12119)
                     /locus_tag="ACHA6A_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8970198.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12308..13069
                     /locus_tag="ACHA6A_002029"
     CDS             12308..13069
                     /locus_tag="ACHA6A_002029"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8970199.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13184..13624)
                     /gene="fabZ"
                     /locus_tag="ACHA6A_002030"
     CDS             complement(13184..13624)
                     /gene="fabZ"
                     /locus_tag="ACHA6A_002030"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8970200.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            13844..14599
                     /gene="fabI"
                     /locus_tag="ACHA6A_002031"
     CDS             13844..14599
                     /gene="fabI"
                     /locus_tag="ACHA6A_002031"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8970201.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(14709..15761)
                     /locus_tag="ACHA6A_002032"
     CDS             complement(14709..15761)
                     /locus_tag="ACHA6A_002032"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8970202.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            15887..16807
                     /locus_tag="ACHA6A_002033"
     CDS             15887..16807
                     /locus_tag="ACHA6A_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8970203.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         4910 a         3637 c         2957 g         5370 t
ORIGIN      
        1 gcttctttaa gaataacatc aagaatagct tcttttttct gcttaatatt ccattaatgt
       61 aatgatgtca tatcctttga ttttgtcgcg tccatggaga tccatcaatt cgatcaaaaa
      121 tgcgcagcct acgactacac cgcctaattg ttcgatcagt tcgatcgtcg ctttgattgt
      181 tcctccagta gctagcaaat cgtcacaaat cagcacacgt tgacctggtt taatggcatc
      241 cttatgcaat gtcaatgtat ctgttccata ttctaatcca taagtcactt cgatggtttc
      301 tcgaggtaac ttcccttttt tgcggactgg ggcaaaaccg actcctaact cataagcaac
      361 aggacatcct acgatgaatc cacgggcttc tggcccaact accatatcaa ttcgtttttc
      421 ttttgcataa tttacgattt gtttagtagc ttcccgataa gcgtcaccat ctgccatcag
      481 tggggaaata tctcgaaaca caattccttt tgacggatag tcgggaatac tggcaatata
      541 atcttttaaa tccactcttt gtttcctcct aggttgttaa ccatttcttt atggtaggaa
      601 tatcgctcaa aagcaaaaat tcctcaactt tgattttatt gagccgtcgc tggtataatc
      661 tgctctcagt caaaggatga ctttctgggc tagcgacttt ttgtaaaaca ccgttctcta
      721 ttgtaacaaa tttcaattca aaaaacacct gtatcataaa aattaataat ttttcaggga
      781 tacgcaaata ttgagctatt gcttttattt tatgacgtat atcaattcgt tcttgagaat
      841 ggatcaattt aaacaatttt gcgtactgtt ctcttgtgcc taccccattc aaataggcct
      901 catcaggtga aatccccatc agataaacac gattgaatga ataagaccga aaaacttctt
      961 tcagatccgc taaatcatct ggacaatcca taacaactag agagtttggt tttctttttt
     1021 caattattgc ctttacagaa tctagtcctt cgaaaacaat cgcagcttcc tggataatct
     1081 caggcacgaa tttcaacgtc gaagaatcaa atgctaaata aaggctacct tccgacgttc
     1141 ggctctgttc acggaaacgt tttgcccgcc aatcaaatac ttgcagacca cccacagaaa
     1201 aatcagatac catcagctgc ggtttcttac gcccgttcca ttcattgatc gaaagctttc
     1261 ctaccacatc tacctgatta ttcagcagtt cttcttcttg agtaccaaaa ccaaaagcga
     1321 ccgcatcaag ctgactcgat gcatcggaaa ggacaaattt cagatgctgt tggtttgctc
     1381 cgattctttt gatattttct gcttggactt gtcgaaataa aaactgaggc aaaggattat
     1441 ccgtaccaaa tggcgccaat agtttcagct catcaattcg ctctactgtt acttcatttg
     1501 gcagcaatac ttcatcaatt ctcaactcag gccctctcat caagtcaatt tgatgatcga
     1561 ctatatattg gttcatcttc tcctgtagta tagtgacatt ttcacttggc atcgttaaac
     1621 cgactgctgc atgatgtcct ccgaagtaag taaataaatc tcgcatctgg tcaagcattt
     1681 caaaaagatt caaggcttca atactacgtc cagatccttt agctgatcca tcttctttta
     1741 aagtaaggac aagcgttggt ttacccgttt cattcattat tttcccagca acaatgccca
     1801 aaacgccttc atgccatcct cggtttacca atagatggat atgattctcc ttattgacca
     1861 tcgctaatgc ttcttgtgtt atctgctcta cgattgcttt tctttcttca ttgatctcat
     1921 tcaatttttt tgcttgaacg gatgcttcct cttcatcaaa cgtcgcaagt aacgagacgg
     1981 caggacgtgg atcgcccatc cgaccgattg catttagtct gggagcaatc gaaaaaccga
     2041 tcgttgtttc gtctgcgtcc ctcattttta cgccgctttc ttcaaatagc gcctgaagtc
     2101 ctatccgttc tgaatgatga atggcatcga ttcctaatga aacaaggatt cgattctcat
     2161 cagtcaatga aactaaatcg gcgatcgttc ctatcgctac taaatccaaa aattctgcag
     2221 gcggttcttc caataatgca gaagctactt tgaatgctac tccgactcct gccaaatctg
     2281 gaaatggata gtgccccgct ggatgacgag ggtgaacaat cgcataagcg tctggtaaat
     2341 tctcaggtag ttcgtgatga tctgtaatga tcacgtctac accagcttgc tgagcgtatg
     2401 ctaccgcatc atttcctgct accccgttat caacagtgat gatcaactgg attccttctt
     2461 cgatcttttc ttggtatacc gattggtttg gtccataccc atgctcaaat cgatttggca
     2521 ggaatacttc tacttctgca ccaagtaatt ccagtgtttc tttcattacc gttgcactgg
     2581 tgatcccatc tgcgtcataa tcaccgtaaa cgaggatttt ttctccactg attacagctt
     2641 cttggattcg gctgattgct ttatccatat cgtgtatcag atacggatca tgtaattctt
     2701 gttcctttgg atgtaaaaat cggttgatat ccttttcctc atggtaacct ctttgccaca
     2761 gtaacttacc gacaaatgga gataatttca gttcacggat catttggaga aaggattcag
     2821 aaggctctgt ctcgcttaac ttccaatcaa aatttgcttg tctcactttc tcactcctaa
     2881 cttagttatt atagcaaagg aaatcagcaa aaaaaagcag aaagaaagaa taaaaacaga
     2941 tcagatccgc tagacagatg ttcagcagga cctgatcttt tgtacgacca tttatgaaat
     3001 atccctatta gtcaaaataa ttgaagcttt gtttgggcga ttgagttttt tgttcttccg
     3061 gcgctttcaa ttgactgatt tgtgcggttt tcgcttgtaa ctccgcctga taagcagctt
     3121 ccaactccgc acgttcgttt ctaaactcgc gttgctgttc ttcttttgct tcttcaattt
     3181 tcttttgaag ttctgtttga tattctgaca tttcctgagt cagctgtttg atttgctttt
     3241 tttgctgcca tagtgttgcc gaagcagtca aaaatacaat cagtgcacca atcagcgcag
     3301 aaccgatgat cactaagatc aatggcgcag aaaaggaagt gaagccaaaa cttacaggaa
     3361 catttttatt gttcaataca gcaaaaagaa caataatcaa aactaaaaca aatccaagaa
     3421 tcacattgct ttgctttttc aattccttca cctacttttt gttaaaaatt ccgctggcta
     3481 aataatcacc aatcgttgga aacagcgtat aaaattttga tgcaacttct aaaataaacg
     3541 gttgattgat ttcccgtttt ggatgtctca tattgcggac gatcattttt gccaacttgg
     3601 caggttctaa aacgatttgt ccgacttttt ccaaatagct tccgcttgga tcagccttat
     3661 cgaagaaatt tgtctcgatt ggacctggat tcactgtcgt aacagctacg ccaagtggtt
     3721 tcaattctaa tcgcaaagcg ttggagaagc caaggactgc aaatttcgtg gcagaataaa
     3781 ccgttgattt tgcagttgcc atttttccag ccatagaggc gatattaatg atatgacctt
     3841 gtcttctttc tgccatatcg atcgcaaatt tttgtgtcaa aaccatcatt cctaagatgt
     3901 tgacttcaaa catatcgtat gcttttccaa ggtcgaatgt aaggaagtct tcgaaaatcc
     3961 caaaacccgc attgtttacc agaatatcga cgcgcccgac ttcttccatc accttttcat
     4021 ataattcgtc cacgctttca ggattgctga cgtctacctt gaaagcataa gcctctttgc
     4081 cgctcaagcg tctgcactcg tctctgacac cttctacaaa tgccattctt cgtgcgcaac
     4141 ttataacgat tgctccttgc tttgctgctt cataacagat ttgtgcgccc agaccgcctg
     4201 aactacctgt tactaaaaca actttatctt ctaaattcat gcctcttcct cctcgcgaaa
     4261 tggaatctca atgctatcaa aatctttcat aatcgtcgtg ttttcaaaca cttcttgtgc
     4321 ttctttttca agctctaaag ctgcttttcc taaatatctc gcactgatat gagtaagtag
     4381 cagccgtttt gcctgcgctt cttttgcaac ttcagcagct tgatgagtcg tggaatggaa
     4441 atagttgtgc gccattcttg cttcatcttt attaaatgtg ctttcatgta ccagaatatc
     4501 gctattttcc gcaagcacaa cggaattatg tgtttttctc gtatcgccta aaatagtcac
     4561 gattcgtcct tttttatctg gacctacgaa atcttttcca ttgatcgtcc gcccatcttc
     4621 tagttggatc gtttcacctt gcttcagttt gccgtacagt ggtccagctg gaatacctaa
     4681 ttcttttaga cgatccacct gcaattctcc tttatgatca tgttcaacca cacgataacc
     4741 aaaactgtcg atcccatgat ttagtttttt tgtataaaca gaaaattgct gatcagtaaa
     4801 aatcggggct gtttcattta gctcaatgaa tttcagcgga taacttaaac gcgtttgcga
     4861 aatacctaaa gagactttta tgtattcctc tatccctttt ggcccgtaaa tctctaatgg
     4921 cgtatcccca ccttgaaatg accggctgct gatgagcccg ggaagtccaa aaatatgatc
     4981 gccgtgtaaa tgtgtaatga aaattttacc gatttttctc ggacgaatag tcgttcgcaa
     5041 aatctgcatt tgcgtgcctt cgccacagtc gaacaaccaa acttcattgc gctcgtctaa
     5101 aagtttcaat gctatacttg tcacatttct atgttttgca ggaacacctg cacctgttcc
     5161 taaaaattgt aattccataa tctacctgac tttctttaag ttcctcttta attttagggt
     5221 aaaaagaagg gataatcaac cgtaagtcga gaatgggttc aaattaaaaa taacgttcta
     5281 aagcgatagc gttttctttg aaaattacta taaaaacagc agcgaacccg tttgagatcc
     5341 tctgctgttt tttattaatt tgaagatcca gaactttcat tgtcgttgga agtttgcgga
     5401 tgtttgtcct tccaagtctt ttgtgcagct tctttcgcat gattttcagc ttctttcgca
     5461 tcatgcacat ctttttcgac atcttcataa tccaagcgag taatttctcc gcctaattcc
     5521 atcattacca attgattcag tgggcgattg tcctcttcat cagcaatcaa gatcagtcct
     5581 gtagctcctt caccgatttt atttcctaca aagtcgaaca cggtctgggc atcttttatt
     5641 tctttggcat cttgtgtcgc accgatcatt cctccggcaa accatcctaa taaaatccct
     5701 agcggaccac ctaatacacc aaccaacatt ccgattaatc cacccgtaga agatttgtta
     5761 ttaccggtaa agtcgataaa atcttctatt ttgaattgat gtgtcccatt aggatcatgg
     5821 ctgacaactg ccatctgttc tcctttaatc gaacgaacag catgtaactt tttgatttct
     5881 gaaaatgcct gatatgcctg gctttctact tgaaaattta aaatgattac tcgtttgctc
     5941 attaaaaacg cccctttcat accttcttgt tatttatttt acctttaatt gaaaaataag
     6001 aaaagaaaag cactcttttt ttccaataaa atagggctgt gacaaaagtc gtatcacagc
     6061 tcctttttcc gaataaacgg cgttcttgaa gctgatcact tctgtcacaa cctcccttaa
     6121 atctgaatct atattttttg gtatttgttt ttatttgctt gcctattcga caaattcaaa
     6181 ttcaaactca ccgatacgta caaggtcgcc atctttcgca ccgcgtgccc gaagagcttc
     6241 atccacaccc ataccacgta attgacgagc aaaacgcatc actgtttcat catgatcgaa
     6301 attcgtcatt tggaataatt tttcgatctt ctcaccagat aagatccacg ttgcatctga
     6361 atcacgatcg atttggaact caggaccttc tggattgaat ccatagtgta cagtttcttc
     6421 ttccagttct tcttcgtaca atgggaattc tggcgttact tctaacaaat ctgctgtagc
     6481 attcaataac gcgtccaatc cttgtcgagt cactccagaa attggaaata ctgggatgtc
     6541 atcagcaaat tcatcttctt tttccttgtt cagctgttct ttgaatttca ctagattttc
     6601 cggtgcatcc ggcatatcca ttttgtttgc cacgatgatt tgcggacgct ctagcaagcg
     6661 taagttataa gtagataatt ctttgttgat ggcaagatag tcttcataag gatctcgtcc
     6721 ttccatccca ctcatatcga tcacatgtaa aattactcgt gttctttcga tatgacgaag
     6781 gaattgtgta cccagcccaa caccttgcga agctccttca atcaagcctg gcaagtctgc
     6841 tgcggcaaaa cttctgccat cacttgtagt aaccattcct agattaggta cgagcgtcgt
     6901 gaaatgataa gcgccgattt ttggacgagc agatgaaata acggatagta aagtcgattt
     6961 cccgacagat ggaaaaccta caagtccaac gtctgccaat actttcaatt ctagttcgat
     7021 ttttcgctct tgtcctggtt caccgttttc agcaatttcc ggtgcaggat tccgaggaga
     7081 ggcaaaacgg atattccctc gtccaccgcg tccacctttt gccacaacta gtgtttgacc
     7141 attttcaatt aaatctccta aaagagcacc agtctccgca tctcggacag tggttccttg
     7201 tggtacttta acataggtat cttctgaacc acgaccgtgc atccctttac tcatcccatt
     7261 ttcacctggc tgcgctttga aatggcgatt gaaccgaaaa tccatcaatg tacgtaaccc
     7321 ttcatcgacg atcaagatga catcgccgcc acgaccaccg tctccgccag ccggtccgcc
     7381 gtcaggaacg tatttttcgc gacgaaatgc aaccattccg tctccgccct tgccagcttt
     7441 cacatcgatc gttacttgat ctaaaaacat ggacataatt aagtcctcct attattcatt
     7501 tatttatttg actgcctgaa tttccacaat aaacaaaagt cttacttatt ttatcggtga
     7561 aatctgattt tgtctatcat ttcagcaaaa agattgttta ccttcttctt tttttatcat
     7621 ttggtaaggt ttccaacctt ttttctgcta ccaagtataa ttttttgaca aaatattctt
     7681 tgcgtagcag aataacagtg tattaattgt attaattgag gatgccgttt cacagcaccc
     7741 ctaataaagg aggaactttt gatatgacag taaaagtagg tattaacgga tttgggcgta
     7801 ttggccgtct ggctttccgc cgcatcaaag aagtctcaga tgatattgaa gtagtagcaa
     7861 tcaacgactt aacaagtcca actatgttag cacaattatt gcaatttgac tctactcatg
     7921 gtacttatcc tggcacagtt actgcaacag aagactcaat cgtagtggat ggcgaagcaa
     7981 ctcgtgttta tgcagaaccc gacgcaagca aaatcccttg ggcaaaagaa aacggtgtag
     8041 atatcgtact agaatgtact ggtttttata catctgaaga aaaagcaaaa gcgcatttag
     8101 atgctggtgt caaacgagta gttatttctg ccccagctgg tgctatgaaa accatcgtct
     8161 ataacgtcaa tgatgataca ttagatgaaa acgacaaaat catctcagca ggttcatgta
     8221 caacaaactg tttagcacct atggcttatt tcttaaataa tgaatttggg atcgaagttg
     8281 gtacaatgac aacagttcat gcctacactt ccacacaaat gctcttagat ggacctgtaa
     8341 aaggcggtaa tttgcgtgct gctcgttcag ctgctgacaa tactatccca cattctactg
     8401 gtgctgcgaa agcaatcggc ttagttatcc ctgagctaca aggaaaatta caaggacacg
     8461 cacaacgtgt tccagtcgtt gatggttcat taactgaatt ggtttcagta ttgaaaacaa
     8521 aagtaactgc tgatcaagta aacgaagcaa tcaaaaaaca cacagttgac aatccttcat
     8581 tcgggtatga tgaccgtcaa atcgtttcta gcgatgtcat tggaacaact caaggttcga
     8641 tctttgaccc aacgcaaaca gaagtaacaa ctgctggtga tttccaactt gttaaaactg
     8701 ttgcatggta tgacaatgag tatggattta cttgccaaat gattcgctta ctagaaaaat
     8761 tcgctaactt gtaatttgat agatcaatcg ataaaagaag cctttgactg gatccagtca
     8821 aaggcttctt tcttttcatc ccctactttt aaactcgttc aatgaacata ttggcttaaa
     8881 aattgttgga ctacttgctg atatttttca ggatctgtct tgtatgcttc cgcatgctcg
     8941 gctcctttta caacatactt ttcttttggt cccttcgttg cacgatagac cttatctaac
     9001 atttcaaatg gtacaaatgt atcttcgtct ccatgtataa agagcattgg gcgctggttt
     9061 ttcttcaatt gttccactgc atttgcttca ccgaaaaagt agccagcacg gacttttgtc
     9121 actagactcg ttacttgcat aagcggaaat gctggaagac taaacatatc gtctagctga
     9181 taagcaagct catcatgaac ggaagaataa ccgcaatctt caatgattgc tttcacatta
     9241 tctggtagtt tttctccgct agtcatcata actgttgctg ctcccatgct gacaccgtat
     9301 agaacgattt cttgcgattt tccattgttt tctagcactt tgtttatcca ctgtacatag
     9361 tctttacgct ctggccatcc aaatccaatg taatcccctt cgctttttcc atgtcctctt
     9421 gcatcaggaa caaggacatt gtaacccata tcatgaaaca tctttgcgta attggtcatc
     9481 gtttcagcgt ttcccatata gccatgagcc acaagagctg ttttttcact tttcgtttca
     9541 gcaggaagat aaatcgctga taatttcaga ccgtcttttg aagtgatggt ccaatcgctt
     9601 cggttttctt ttgaactaaa ccatttatgt gcatccgttt ctttggatgt ctgtgttgta
     9661 ccaggtgtat cttcgtctaa aaagtctttc tcagaaggca caacagcata attataaaaa
     9721 taattccctg caaacactaa cgcaacaatc agagctaaag cgacgcctaa aaggattttc
     9781 atccaaagtt tcataaatca tccatctcat ttctcttttt tcatataacc ttgaattagt
     9841 ataatcaaag aatctctact atgcaatgtt catttttata acccgcgaat tgtaaaatta
     9901 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     9961 caaaggagtg taaccataaa tgacctacaa acatcttacc atagacgaac tgacaatgat
    10021 agaatcatat tatcttcaac ataataagcc ggttgaaatc gctaaccgaa tgggacgtgc
    10081 tatacaaact atttataatg tagtcaataa gttcaagcaa ggcaagactg ctcttgatta
    10141 ttggcaccag tataaagaaa ataagaaaaa atgtggtaga aaagtcattc aattacctgc
    10201 tcatgaagta gattacatta aagagaaagt cactcttggt tggacgcctg acgtcattat
    10261 cgggcgaaaa gaaaggtctg tttcatgcgg tatgagaaca ctttatcgtt tattttctaa
    10321 aggaatattt gatattgaca cactaccgat gaaaggtaaa agaaaaccca atggccatca
    10381 ggaaaaacgg ggaaaacaac agtatcagcg ctcaatccat gatcgacctg ataattatcc
    10441 tgatttcaat tctgagtttg gtcaccttga aggtgatacg atcgttggca ttcatcataa
    10501 aagtgccgtc attactttag ttgaaagatt atctaaagtc attatcacga ttaaacccaa
    10561 cggccgtaag gcattagata ttgaaactgc ccttaatcaa tggttttctc gcttccctaa
    10621 aaacttcttt aaatctatta cgtttgactg tggaaaagaa ttttctaact ggaaagccat
    10681 tagtaaccaa catgatattg atatatattt tgcggaccct ggaacacctt ctcaacgccc
    10741 attaaacgag aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt
    10801 tagaaaagtg aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag
    10861 aaaatcattg aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt
    10921 ttattctagc ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa
    10981 taactggcta tttctagcgg attatttctg caataactaa ttatctattt tttattttca
    11041 attaatttaa agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag
    11101 attcttccta caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt
    11161 tctcctttgt attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct
    11221 cgcgtattat gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga
    11281 aggatatatt ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt
    11341 tcgttttctg taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat
    11401 gcttctgcca tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca
    11461 tcaatcaatc ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg
    11521 tcttccagcg tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa
    11581 ttgacgaatg ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag
    11641 tgttcgtatc ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta
    11701 tccgtgtttg gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg
    11761 atctcgattt tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat
    11821 ccgatatcta agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa
    11881 gaaatctcca actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg
    11941 ctattcgcgt aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg
    12001 atctgcagca aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac
    12061 gctttgtcct tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata
    12121 ttaccatttc ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa
    12181 gtaaatggtc tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa
    12241 gtaggtattt tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg
    12301 aacagttatg aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt
    12361 tgctgctggt tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa
    12421 aagtacatct actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt
    12481 accacaattg acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa
    12541 aggcacaatc aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat
    12601 gacacatgca aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt
    12661 catgatccaa ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga
    12721 aggatttgaa acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc
    12781 tcgctcacaa gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga
    12841 tatgcatgat ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa
    12901 cggcggttat ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat
    12961 ggatgtcgtg gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga
    13021 agtaaaaatc gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt
    13081 ctgaatcctg catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa
    13141 agcaagtaag cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga
    13201 cgatgaacgt gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg
    13261 ctgtaccgac tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg
    13321 gaacaacttt ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta
    13381 gaaactcttc tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca
    13441 acgcacccgg catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg
    13501 ttacattttt tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat
    13561 aacaaatagg ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag
    13621 acataattaa actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta
    13681 tcatattcct aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca
    13741 aaacattttg actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt
    13801 tttacgacta tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg
    13861 aaaaaaaatc gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga
    13921 agcaatgttg aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa
    13981 atcattagtc aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga
    14041 cgaaagcatc gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct
    14101 tgtccatgca gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc
    14161 cagagacggc tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa
    14221 atacgcacaa ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc
    14281 agaacgcgca atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc
    14341 tatccgttat ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc
    14401 tggggcaatc aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt
    14461 atctgaatct cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg
    14521 tgctttctta atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa
    14581 aggtgtccat ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag
    14641 gaaaaaactt tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta
    14701 ctttgaaatt attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga
    14761 atacctgtca attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa
    14821 aatacatgaa gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta
    14881 ccacatgcac cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt
    14941 gcttgcagtc ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt
    15001 aagatagagg gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt
    15061 gctgcaccta cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag
    15121 taatccagtt cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact
    15181 tttgctgcac cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt
    15241 ggttttagtc cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat
    15301 tctggagctg gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat
    15361 aatgtagcgc ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg
    15421 acagcatcaa cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga
    15481 ttcatccgtc gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga
    15541 taagctttta gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga
    15601 atcgtcaggt aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg
    15661 gcaataaaac gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc
    15721 atatacttac caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat
    15781 tttattttca gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact
    15841 tcttggtaca atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt
    15901 agcaactgcc ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa
    15961 agtaaatgcg tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt
    16021 tatccaaaaa agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt
    16081 gggaaaatat ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga
    16141 gagccgctta aagactttac atagtctttt aaatactatt tttcattata cccttggttt
    16201 cttttttatc tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg
    16261 tgccggaatt gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat
    16321 taccggattt tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc
    16381 aaacttaacg attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc
    16441 aacggatggt actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc
    16501 tcgtgcaaat atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa
    16561 aatctatgag atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct
    16621 ccaaactgaa ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg
    16681 tacggtctgc tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc
    16741 ctatgtcaaa gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg
    16801 aaaataattt ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa
    16861 agaacacttt tgtc
//
LOCUS       DAYYHS010000063        16498 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000063 DAYYHS010000000
VERSION     DAYYHS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.063"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACHA6A_002034"
     CDS             complement(159..1469)
                     /locus_tag="ACHA6A_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8970204.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="ACHA6A_002035"
     CDS             complement(1481..2899)
                     /locus_tag="ACHA6A_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8970205.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="ACHA6A_002036"
     CDS             complement(3139..4707)
                     /locus_tag="ACHA6A_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8970206.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="ACHA6A_002037"
     CDS             complement(4711..5121)
                     /locus_tag="ACHA6A_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8970207.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="ACHA6A_002038"
     CDS             complement(5118..6041)
                     /locus_tag="ACHA6A_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8970208.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="ACHA6A_002039"
     CDS             complement(6038..6916)
                     /locus_tag="ACHA6A_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8970209.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="ACHA6A_002040"
     CDS             complement(6903..8240)
                     /locus_tag="ACHA6A_002040"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8970210.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="ACHA6A_002041"
     CDS             complement(8243..9346)
                     /locus_tag="ACHA6A_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8970211.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="ACHA6A_002042"
     CDS             complement(9420..10928)
                     /locus_tag="ACHA6A_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8970212.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="ACHA6A_002043"
     CDS             complement(10894..11586)
                     /locus_tag="ACHA6A_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8970213.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="ACHA6A_002044"
     CDS             complement(11608..12582)
                     /locus_tag="ACHA6A_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8970214.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="ACHA6A_002045"
     CDS             12882..13754
                     /locus_tag="ACHA6A_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970215.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="ACHA6A_002046"
     CDS             complement(14212..16410)
                     /locus_tag="ACHA6A_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970216.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4869 a         3466 c         2677 g         5486 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatttgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaacttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttc
//
LOCUS       DAYYHS010000064        16377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000064 DAYYHS010000000
VERSION     DAYYHS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.064"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..554
                     /gene="hslV"
                     /locus_tag="ACHA6A_002047"
     CDS             6..554
                     /gene="hslV"
                     /locus_tag="ACHA6A_002047"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8970217.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            568..1968
                     /gene="hslU"
                     /locus_tag="ACHA6A_002048"
     CDS             568..1968
                     /gene="hslU"
                     /locus_tag="ACHA6A_002048"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8970218.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1986..2777
                     /gene="codY"
                     /locus_tag="ACHA6A_002049"
     CDS             1986..2777
                     /gene="codY"
                     /locus_tag="ACHA6A_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8970219.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2859..3734
                     /locus_tag="ACHA6A_002050"
     CDS             2859..3734
                     /locus_tag="ACHA6A_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8970220.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3866..4504)
                     /gene="plsY"
                     /locus_tag="ACHA6A_002051"
     CDS             complement(3866..4504)
                     /gene="plsY"
                     /locus_tag="ACHA6A_002051"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8970221.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4631..5065
                     /locus_tag="ACHA6A_002052"
     CDS             4631..5065
                     /locus_tag="ACHA6A_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8970222.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5231..7276
                     /gene="parE"
                     /locus_tag="ACHA6A_002053"
     CDS             5231..7276
                     /gene="parE"
                     /locus_tag="ACHA6A_002053"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8970223.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7288..9738
                     /gene="parC"
                     /locus_tag="ACHA6A_002054"
     CDS             7288..9738
                     /gene="parC"
                     /locus_tag="ACHA6A_002054"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8970224.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10015..12246
                     /gene="pflB"
                     /locus_tag="ACHA6A_002055"
     CDS             10015..12246
                     /gene="pflB"
                     /locus_tag="ACHA6A_002055"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8970225.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12490..13251
                     /gene="pflA"
                     /locus_tag="ACHA6A_002056"
     CDS             12490..13251
                     /gene="pflA"
                     /locus_tag="ACHA6A_002056"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8970226.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13336..14262
                     /locus_tag="ACHA6A_002057"
     CDS             13336..14262
                     /locus_tag="ACHA6A_002057"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8970227.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14444..15337
                     /locus_tag="ACHA6A_002058"
     CDS             14444..15337
                     /locus_tag="ACHA6A_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8970228.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15471..15596
                     /locus_tag="ACHA6A_002059"
     CDS             15471..15596
                     /locus_tag="ACHA6A_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8970229.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15730..16362)
                     /locus_tag="ACHA6A_002060"
     CDS             complement(15730..16362)
                     /locus_tag="ACHA6A_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970230.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5817 a         2791 c         3492 g         4277 t
ORIGIN      
        1 caaacatggt cgaatcacca tttcattcaa ccactatctg tgccgtcgaa aaagacggga
       61 aatttgctat ggctggagat ggccaagtaa caatgggtga atctgttgtc atgaaaggca
      121 ctgcaaagaa agtacgacgt atctacaatg gagaagttgt tgtaggtttc gccggaagtg
      181 ttgcagatgc gtttacttta gaagaaaaat ttgaaggcaa actaaacgaa tataacggga
      241 atctacaacg cgcagctgtc gaattagctc aagaatggcg gacacagcaa tctatgcaaa
      301 aacttgaagc gatgctgatc gtaatgaatg ataaagaaat gcttcttgtt tcaggtacag
      361 gagaagttat cacaccagat gatggtatct tagctatcgg atcaggaggg aattttgcgt
      421 tggctgcagc ccgagcaatg aaaaactatg ctcaaaacga catgagtgcg aaagaaattg
      481 cagaaaatgc gttaaatatt gccgcagata tctgtatctt tactaatcat aatattatcg
      541 tagaagaact gtaagaggtg agtagatatg tatgaattaa aacaaacacc aagacaaatc
      601 gtaagtgaat tagatgaata tatcgtagga cagcacgcag caaaaaaatc tgttgccgtt
      661 gctttgcgta accgctatcg ccgtttgcag ttagatgaaa aaatgcaaca agatattaca
      721 ccaaaaaata tgctgatgat cggacctaca ggggtcggta aaacagaaat cgcacgacgt
      781 ttggcaaaaa tcgtcaatgc accatttgtc aaagtcgaag caacaaaatt tactgaagtc
      841 ggttatgtcg gaagagatgt agaatcaatg gttcgggatt tagtagaaaa tgcgatccaa
      901 atcgtagaaa aagagcagta cagccgtgta tacacaagtg ctaaaaaaaa tgcagaaaaa
      961 cgtttggtaa aaatcttggt acctggtatc aaaaaagaac agaaacaatc cactaatcaa
     1021 tttgaacaaa tgatgaatct ttttaatgct agtcagcaaa ataccgctga accgcaagaa
     1081 gaagtgacag aagatattcg aatcaatcgc cgtacagtct tggaacaact acaaaagggt
     1141 ttactggata gtcgtgaagt aaccattgaa gttgaagacc aaaagaaacc tgcaccaatg
     1201 atgaacaatg gattggagca aatggggatc gacctaaatg aaactctagg tgctttgaca
     1261 ccgaagaaaa aagtggaacg gacggtaaca gtcaaagaag ctttagagtt attgatcaag
     1321 gaagaatcag caaaattagt caaggatgct gatatccata gtgaggcaat caaattagcg
     1381 gaagaaagtg gaattatttt tatcgatgaa atcgataaga tcacttctaa atcacagcaa
     1441 tctggagaag tatccagaga aggcgtccaa agagacatat tgccaattgt cgaaggttca
     1501 caagtaaaca caaaatacgg gatcatccaa acagatcacg tcctattcat tgcttcagga
     1561 gctttccatt tgtctaaacc aagtgatcta attccagaac ttcaaggacg cttcccgata
     1621 cgggtagaat tagatgattt gacagctgaa gattttgtga agattctgac cgaaccaaat
     1681 aatgctttag taaaacaata cgtcgcgttg atcggtacag aaaatgtgtc agttacgttt
     1741 acaaaagaag caattgaacg gatcgctcaa atcgctttcg atgtcaaccg tgacacagat
     1801 aatatcggcg cacgcagact gcataccatt ttggaaaaac tactagaaga tctattattt
     1861 gaagcaccag atatgcaaat gggagaaata acgatcacag aagcatatgt ggatgacaaa
     1921 cttggctcaa ttgcggcaaa tgaagattta agtcgctata ttttataaaa aatcggagga
     1981 cgacaatgac agacttgcta acgaaaaccc gcaagataaa cacacttttg cagcaaaaga
     2041 atacttttga catgcaagca gatttgcctt acgataaaat ggctgtcaca ctaggtggta
     2101 ttcttgacag caacacctat atcataggaa gtgacggagt attactaggt tataatgaac
     2161 gtcacgatgt gaacaatgaa cgaatcaaaa caatgtttgt tgagaaacaa tttcctgaat
     2221 cttatactga tacagtggca cagttgatga aaactgaagc taatatccca attactagtg
     2281 atattactgc atttccggta gagtcaagag agaaatatcc atttggtctg acgactgtcg
     2341 ttccgatctt tggagctggc gaacgtttgg gtacgattat cttatctcgt atggaacaat
     2401 cgtttgcaga tgacgatcta gtcttagcag aatatggagc tacggttgtc ggtatgcaga
     2461 tcctttatca gaagtctcga agcatcgaag cagatgtacg gagtgcaacg gctgtccaaa
     2521 tggcaatcaa cacattatct tatagcgaat tgaaggctgt acaagctatc tttgaagcat
     2581 tggatggaga tgaaggtcgt ctgactgctt ctaatattgc cgatagtatc ggcatcactc
     2641 gttctgtgat cgtcaatgct ttacgaaaat tggaatcagc tggaatcatt gaatctcgtt
     2701 cgttaggcat gaagggaact tatttaaaag tattgaattc ccgttttaaa gacgagttag
     2761 caaaacacag ttattagaag taaggggact ggggcagata gaaatgtttg ttccagcctt
     2821 cccattttaa aaagtaagtc gaagttggag gaatggcaat gactgttatt attcaaaatg
     2881 accagctgat cgccgaaatc gctgaacacg gtgcggaatt gatcagtctg aaatcaaaag
     2941 aacatggttt agagtatatc tggcaaggtg atccagcgta ttggggcaga catgcaccgg
     3001 ttttattccc ttttgttgga cgtttaaaag atgatcagta tgtttatcaa gggaaaacat
     3061 atcaaatggg gcaacacgga tttgctcggg atatggattt tgaagtcatt gaacatggac
     3121 aagaaatggc tagtttcatc ttaaggagta cgccggagac attagaaaag tatccatttg
     3181 attttgaatt agtgatcagc tatgaattag gaggagacgg catcacggtt cattatcaag
     3241 tggcaaatac cggtgaaaaa gaaatgttct tttctatcgg tggacatcct gctttcaatg
     3301 tacctcttga agaaggattg tcatttgaag atttctatct ggcattttct ccattgaaat
     3361 caagattacg cctaccattg aaaggcccat tcatcgatat ggatcaaaag acgttaggtc
     3421 agacaaatac gaatctggct ttgaatcgtg aactttttga gcaagatgcc ttggtttttg
     3481 aaacaagggg tctaaatgcg tacgccattc gaagtgaaaa atcttcccat agcgtaactt
     3541 taagctataa agatatgcct tatgtaggga tctggtctcc ttatccaaaa gaagcgcctt
     3601 tcgtttgtat cgaaccgtgg tgcggaatcg ctgacacggt gaacagtagt ggaaacttga
     3661 tggaaaaagt agggatcaat caactaggtg cccatgaaat cttcaagaca aaatattcca
     3721 tcacagtgaa gtaagaggtt gtgaccgaga agttagcagg gaacaccgtt taccaaggtg
     3781 tgtaaaaaag ctatcctgta agtaataagg gagtgtgaca tcgatttgtc acactcccgt
     3841 tttatctctt tggaatgaaa aagtatcagg attttttctt gctgctattc aaaccaaacg
     3901 ggattcgact ttcagtatgg tttctgatac gcttgatatt gtctttatgc cgataaaaga
     3961 taaaggaaga aactaaaaag gcaatgatcg tcagtaacca attgggctta gccaaaatca
     4021 caggagctac ataaggaagg acgatcgtag ataaagtgat caatactgca ctgatcatac
     4081 tgctcaaact caccatactt gtacaaaata aactaacgat aaagatgata caagaataaa
     4141 caaaaaatac aggattaaat cctaaaagca ttcctgcact tgtcgcaaca gcttttcctc
     4201 ctttgaaccc agcaaaaatc ggaaatgtat ggccaagaat ggcgcatacc ccaaaccaaa
     4261 gagggttaat cccagaaagg tggaagatca tcggcaaaca agtagccaat gtacctttta
     4321 aaatatccat aagcaggaca acaatcccgg ctgttttgcc taatacacga aatgtattgg
     4381 tcgttccagt attaccgctc ccgtgttgtc ggatatcttt tttgaaaaac aatttgccaa
     4441 tccagacacc ggaaggaata gaacctaaaa gatatgctaa aataagtaat aagatcaatt
     4501 tcatttttta ccctctttca agttgaaaac tatcctttat tttagcatga ataggtaaca
     4561 ggaacaattg aaaaaatgaa agatacaagt ataatagatg gaataaaaag aaaagcaagg
     4621 ggtggcagaa atgtctgtaa aaaatccaac acaagaaacg attttcaact atttggaaga
     4681 agcaaaaaat attgccgtcg taggcttgag caataaacca gaacgaacaa gttacatggt
     4741 tgcacaggta ttgcaagaca atggatataa aatcatacca gtaaatccat tattggctgg
     4801 tgaagaaatt ctaggcgaga aagtctatgc gaatttgaca gaaatcgatg atccaatcga
     4861 tatcgtagat gtatttagac ggagtgaatt tttacctgaa atagcaggtg attttttgaa
     4921 aactgatgct aaagtgttct gggcacaatt agggattgaa aacaaagaag cagctgagat
     4981 gctaagaaaa gcaggacgta cagatatcgt aatggatcgt tgtattaaga ttgaacttgg
     5041 taagttgaaa gaaaaccaaa aataaaaatg accacaaaga agagatgatc gatagagggg
     5101 aatgtcgaaa aagcggttag gagataatta aaattgattt ctcgtctacc ctcttttttt
     5161 tcacgacctt ttctagtatg ataatcgtgg tgcggttttg ccgcaaagta ttattgaagg
     5221 agcgtttgcc ttggcaaaaa aagtaaacaa cgaatacaat gactcgtcca tccaagtatt
     5281 ggaaggatta gaggcagtta gaaaaagacc agggatgtac attggatcca ctgacagccg
     5341 aggcttgcat catttagtct atgaaatcgt cgataatgca gttgacgaag cattatcagg
     5401 ttatggcagt gagattgatg tgaccatcca tgaagacaat agtataactg ttgcagatag
     5461 cggacgtggg atgcctgtag gaatgcatgc ttccggaata ccaacagttg aagtgatttt
     5521 tactgtgctg catgctggcg gaaagtttgg tcaaggaggg tacaaaactt ctggtggact
     5581 gcacggtgta ggagccagtg tggtaaatgc cttatcgaaa tggctaattg taacgatcgt
     5641 tagagatggc gtagagtatc agcagaaatt taaaaacggc ggtaaacctg acggtacttt
     5701 gaaaaagata ggaaaaacaa aaaaagcaaa cgggacgact gtccattttt taccagatga
     5761 tacaatattc agtacaacga aattttcata tgaaatttta gccgaacggt taagagaatc
     5821 tgcctttctt ctaaaaggcg ttaaaatctc tctttctgat cttcgtggag aagaaccagt
     5881 caaggaaatt tttcattacg aagaaggaat caaagaattt gttgattatc tgaatgaaga
     5941 aaaagataca ttgacaccag ttgtttattt ttcaggagaa aaagaaggta tcgaagtaga
     6001 ggtcgcttat caatacaatg acggttattc agaaaatgtt ttatcttttg tcaataacgt
     6061 tcggacaaaa gatgggggaa cccatgaagc tggtatgaaa gcggcgatga cgaaatctta
     6121 taatgaatat gcgagaaaag ttggtttgct gaaagaacgg gacaagaatc tggaaggtag
     6181 tgatttcaga gaagggttag ctgctgtact ttctatccgt gtacctgaaa atcttctgca
     6241 atttgaaggg caaacaaaag aaaaacttgg tacgccagtt gctcgtaccg tagtagataa
     6301 cgtcatcagt gaacaaatgg gcttttattt gcaggaaaat agcgaaatga gccagatgtt
     6361 ggtccgtaaa gcaatcaaag ccagagaagc gagagaagca gcacgtaaag ccagagaaga
     6421 aagccgaaat gggaagaagc gtaaaaaagg cgaatctctt ctttcaggaa aattgacacc
     6481 cgcacaatcc cgaaacccta agaaaaatga attgtattta gtcgaagggg attctgctgg
     6541 cggatctgct aaacaaggaa gagacagaaa gttccaagca attctacctt tacgaggaaa
     6601 agtcatcaat accgaaaaag ctaaaatgca agatatcttg aaaaatgaag aaatcaatac
     6661 gatgatctat acgattggtg caggagtcgg tcctgagttt tctatcgaag actgtaatta
     6721 cgacaaagta atcatcatga ccgatgcgga tacagacggc gcccatattc aagtattact
     6781 gcttactttc ttttatcgtt atatgaaacc gctgattgaa gctgggaaag tatatatcgc
     6841 gttaccgcct ttatataaag tatccaaagg acaaggaaag aagcaagtca ttgaatatgc
     6901 gtggacagat gacgaattgg cagcaatgat taaaaaagtc ggcaaaggat atatgctgca
     6961 gcgatataaa ggacttggag agatgaatgc ggaacaattg tgggaaacaa cgatggatcc
     7021 gacttcccgt acgttgatcc gcgtccgtat cgatgatgct gcgcaagctg aacgtcgagt
     7081 aaccacactg atgggtgaca aagtagaacc tcgaagaaaa tggatcgaaa atcacgtgca
     7141 atttacgctc gaagaagatg gcagtatctt agataaaaaa gaggatactg aaatctctcc
     7201 ttcagtttct aatgatttat tagatgaaga acgagcagat aaaaatgaaa acaatcagtt
     7261 atttgaagta gaataggagg tcacctgatg gaaaatcgac aagaagtaca agaattaact
     7321 ttagaagaag tgatgggtga ccgcttcgga cgttattcca aatatattat ccaagaacgt
     7381 gcactgcctg atatccgtga tggattgaag ccagtgcaac gccggatatt atttgccatg
     7441 aacaaagatg gcaatacgta tgacaaagga ttccgtaaat cagcaaaatc tgtcggaaat
     7501 atcatgggga attatcatcc tcatggtgac atcagtattt atgaagcgat ggtccgcatg
     7561 agccaagact ggaagttgcg ggaagtactg atcgaaatgc acgggaataa cgggagtatg
     7621 gacggtgatc cacctgctgc gatgcgttat actgaagcca gattatccaa gttaagtgga
     7681 gagatgctag cagatatcga gaaagaaaca gtcgatctcg tgtggaattt tgatgataca
     7741 gaaaaagaac caacagtcct tccagctcga tacccaaacc ttctcgtcaa tggatcgaca
     7801 ggtatatctg ctggatatgc aacagaaatc ccgactcata acttagctga agtgatcgat
     7861 ggcacaatct atatgatcga tcatccacaa gcgagccttg aaaaattgat ggaatatatt
     7921 cctggacctg actttccaac aggagggatc ttgcaaggaa aagatgaaat caaaaaagct
     7981 tatgaaacag gacgaggaaa agttattctt cgttcgaaaa caaagataga ggcaatgaag
     8041 ggtaataaac agcaaatcgt gatcacagaa atcccatatg aagtaaataa agcgaccttg
     8101 gtgaaaaaaa tggatgagat ccgtttgaat aagaaaatcg atggaatagc tgaagtacga
     8161 gatgaaagtg atagaacagg tctgcaaatt gtcgtcgaat tgaaaaaaga cgccaatgct
     8221 caaggaattt tgaattattt gttcaaaaac actgagctac agatcaatta caatttcaat
     8281 atggtcgcta tcgaccatat gactccgcat caagttggac tgaaagacat tttaagaagt
     8341 tatatcgagc atcgaaagca agtcataaca aaacgtagtc aattcgattt agccaaagct
     8401 caaaaaagac agcatatcgt agaaggattg atgaaagctt tatcgattct ggatgaagta
     8461 atcgcaacga ttcgtgaaag taaagacaaa aaagacgcca aaaagaattt agtcgacgtc
     8521 tttcaattta cagaagaaca agcagaagca attgtcacat tgcaattata ccgtttgacg
     8581 aacacggata tcacagagct tcaaaaggaa tccgaaagct tgattgcgca aatcacagag
     8641 ttgaataaaa tcctttcaaa tgacaaagag cttttctctg ttatgaaaaa agaattgcga
     8701 gaagtgaaaa aaaattattc ttccgctcgt ctaacgacaa tcgaagacga aatcgaagaa
     8761 atcaaaattg atacgcaagt cttagtggca caagaagacg taattgtcag tgtgacgcga
     8821 gaaggctatg tgaagcgaac aagccttcgt tcttatagtg cttccaaacc agaagaaatc
     8881 gggatgcgag aaggcgacta cttgctatac tcaggtgaat tgagtacatt ggaccatgtt
     8941 cttctgatca caaataaagc aaatgtgatc taccgccctg tccatgaact acccgatttg
     9001 aaatggaaag atgctggtga gcacatttct caaacaatct ccaatctgtc tgtagacgaa
     9061 tcgattttag ctgttttccc atatcaaaaa attcaagcag agaaaacatt tgtattcatc
     9121 agcaagaatg gattgatcaa gcaaacacga atgacagagt tcgaaccatg gcgcacctat
     9181 aagagccgtc cgctcagctg tatgaagcta aaggcggaag aagatgaact gattgctgtg
     9241 tatctgaaag aagatcagcc aggcttagat gttttcttag taacgcatca aggaatgggt
     9301 ttgcgctatc ctttaacaga tgttcccgtt gttggagcaa aggcagctgg agtgaagtct
     9361 atgaatttga aagatggaga taggattatc aacgggctgc ttgtgttagc agaaggtgat
     9421 acgccgatcg taatcgttac tcagcgagga gctgttaaac gcatgcttgc tcaagaaatc
     9481 agccaaactt cacgtgcgaa gcggggagtg acggtcatgc gtgagctgaa aaaaaatccg
     9541 caccgtatta tctatatgtc ggaaggaaga tcaaaagaaa taaccttgat gaatcaaaaa
     9601 gggcagcagt taacgattga cccaactgat ttccctatag gtgatcgaac atcgaatgga
     9661 tcatttgcga tggatgagaa aaaaggtgga gaagtcatcg aagttatcga tgctcctgag
     9721 attttcatcg agtcgtaact gttgtagtat tttaatgaag agacgccatc tgtattaata
     9781 caggtggcgt ctctttttca ttcattttta ataaatcctt atacgtttgc aaaaaaagat
     9841 gaaagtgttc acaatgctta aatgttggta tgtcaacgtt gatttaaata tatgtaaata
     9901 aaacataaaa attgttaaat aaaaaacaag aaaacccttg caaagatagg gtgctgtgtt
     9961 atactaatca cgtaaaaact gttacataaa acagaaattt aggaggtcca tacaatggag
    10021 caatggaatg gatttaaagg taccaaatgg aaagaaggcg tagacactag agactttatt
    10081 caaaataact acactgaata tcgcggtgat gacagctttt tggaaggccc aacagaagca
    10141 acagaaaaat tatgggatga attgcaaaag ttaaatgata tccaattcga gcgtaacggt
    10201 gtctatgata tggacacaga aatcgtatca actattactt cacacgatgc aggctatttg
    10261 gataaagatt tggaaaaaat cgtcggtcta cagacagaca aacctttgaa acaagcattc
    10321 atgccgttcg gcggaatcaa tatggctaat aatgcgctaa aaagcaatgg ctatacacca
    10381 aacgatgaat tgacacatat tttcactgac tggcgcaaaa cacataatca aggtgtattt
    10441 gacgcttata ctgctgaaat gcgtgctgct cgtaaaaaca aaatcattac agggcttcca
    10501 gacgcttacg gtcgcggacg tatcatcggt gactatcgtc gtgtggcatt gtatggaatc
    10561 gactatttga tggaacaaaa gaaaaaagat catgatatga ctggttatca tacaatgagt
    10621 gaagaagtga ttcgtttacg tgaagaaatc actgaacaat atcgtgcatt agcgcaaatg
    10681 aaagaaatgg ctgcgaaata tggctatgat atttctaaac cagctgcaaa tgcaaaagaa
    10741 gctgtccaat ggctatactt cggatattta gcagcaatca aatcacaaaa tggtgctgcg
    10801 atgtcaatcg gacgtatctc agcattcctt gatatctata tccaacgtga tttggataat
    10861 ggagtaatca cagaaaaaga agcacaagaa ttgatcgacc acttagtaat gaaattacgt
    10921 atggtcaaat tcgcgcgtac acctgaatat aaccaattat tctcaggatt ccctatctgg
    10981 gctactttat ccattgcagg tatgggatta gatggtcgtt cattagttac aaagaatgat
    11041 ttccgtctgc ttcatacatt aacaaatatg ggaccttcac cagaaccaaa cttgactgtt
    11101 ttgtattctg aacaccttcc agaaggattc agaacatatg ctagcagaat cgctatcgaa
    11161 agttcttcga ttcaattcga aaatgataat cagcttcgtg aacaatgggg ttctgatgat
    11221 tgtgcgattg cttgctgtgt atcaggaaca gttgtcggaa aagacatgca attctttggc
    11281 gctcgtgcta acttggctaa aacactatta tatgccatca atggcggagt cgatgaagtg
    11341 actggcgccc aagttggacc agaatatcgt ccaatcacat cagaaatact agactttgaa
    11401 gaagtgaaac ataacttcat gaatatgatg gattggttag cagaactata tgtcaatacg
    11461 ttgaacatca ttcattatat gcatgataaa tattcttatg aagcagctca gctggctttg
    11521 atggattctg acttgcaacg cacatttgct acaggtattg ctgggatctc tcatgcggca
    11581 gacagcttgt cagcgatcaa atatgcaaaa gtaaaagcaa tccgtaatga aaaaggaata
    11641 gcagttgatt ttgaagtgga tggcgacttc cctaaatacg gaaatgatga tgaccgtgca
    11701 gatgaattag tttcatggat cttggaatac ttcatgggac aaatcaaacg tcataaaact
    11761 taccgcaact caaaaccaac aacttcatta ttgacgatta cttcaaatgt tgtttacggt
    11821 aaaaatacag gaaatactcc tgatggccgt gaagctggaa aaccacttgc gccaggagca
    11881 aatccaagtt acaatgcgga acaaagtggg ttattagcta gcctgaactc aacagctaaa
    11941 atgaattacg gctgtgcatt ggatggaatt tcaaataccc aaacaatcaa tccttcaggt
    12001 cttggaaaag aacaagatac aagaatcgaa aacttgcgta atgtcttaga tggatacttc
    12061 tcaaaaggag cttatcattt gaatgtcaat gtattcagta atgatttatt gcgtgatgca
    12121 atggaaaatc caatgaaata tccgaacttg acgatccggg tatctggtta tgcagttaaa
    12181 ttccgtgact tgacaagaga acaacaatta gatgtcttga tgagaacttc acacgacaga
    12241 atgtaaaaag ttcgtgaaaa aagtttttcg acttagcgtc gaaaggacgt tgaatagcca
    12301 tcgcatcaaa atcgaataat cagtatgctt atttgaaaga aacgatttac aggggcagga
    12361 cttgtatgtt ccgtccctgt aaattttaca cataacgaag cggcaattgc gaaaaatttt
    12421 gttcagaaag cttcgcttcg acagctaact tgtacttaaa atgaaatcaa agaaaagatg
    12481 tgaacttaaa tggaagaaaa aacaatcggc tatgttcatt ctatcgaaac tttcggttca
    12541 gtagacggac ctggactgcg ctttgtcgtg tttatgcaag gatgccgcat gcgttgccaa
    12601 ttttgtcaca atccagatac ttggaatatt ggcggaggga aagaatacac agcagatgaa
    12661 cttttagata aagcagaacg cttcagacct tactggggag ataaaggtgg tattactgtt
    12721 agtggtggag aacctctatt gcagatcgat tttttgatcg aactatttaa aaaagcaaaa
    12781 gagcggaaga tgcatactac attggataca tgcggaaaac cttttacgta tgaagaacct
    12841 ttcttttcac gttttcaaga attgatgaag tatacagatt tactgttatt tgacatcaaa
    12901 cacatcgata atgaagagca taaaaaacta acgcattggg acaatgaaaa tattttagaa
    12961 atggctcaat atttgtcaaa aatcaacaaa ccggtttgga ttcgccatgt actagttcct
    13021 gagcgaagtg attatgatga atatttgatc cgactagaca actttattca aaccttgtca
    13081 aatgtagaca gagtagagat tcttccttat catacgatgg gaaaatacaa gtgggaaaca
    13141 cttggtttga aatatccatt agaagggatc gaaccaccta caaaagaacg tgtcgaaaat
    13201 gctaaacgat tgcttcatac agaagagtat acaaaatatt taacgcgata ggtcgcatcg
    13261 gaaagaagat aagtattgct tctttccgtt tttttgctat tattaaatag gagaaatcac
    13321 aaaggagcga atgacatgtc aaaaatttta gtttttggtc accaaaatcc agatacagat
    13381 gcaatcgggg cagccattgc gttttcacac ttgcaaaatg aattaggtaa agaaacagaa
    13441 gcagttgctc taggtacacc taatgaagaa acacaatacg cattagacca ctttcagcta
    13501 tctgcaccac gagtagtaga gtcagctaaa gcagaaaatg cagatgtcat gcttgtggat
    13561 cataatgaat tccaacaaag tatttcagat atcagcgaag taaatatttt agctgtagta
    13621 gaccaccatc gtattgcaaa ctttgaaaca gctaatccat tgtactatcg tgcagaacca
    13681 gttggatgta caagtacgat tattttaaaa ctatataaag aaaaccaagt aacaattcct
    13741 aaagaaatcg caggaatcat gctctcagct atcatttcag atacattgtt gttcaaatca
    13801 ccaacatgta cgcctgaaga tgaagcagct gcaaaagaac ttgcagcaat cgctgaagtc
    13861 gatctaaaca cttatggact tgaaatgttg aaagcaggaa caaatctaag tgataaatca
    13921 gctgcggttc ttcttgattt ggatgcaaaa agtttcccaa tgggcagttc caacgttcgt
    13981 atcgctcaag tcaatacagt ggaccttaat gaagtgatgg atcgacaagc agagctagaa
    14041 gcagcaatgg aagatgaaat gaaagcaaat ggctacgatc tgttcgtatt agttgtcaca
    14101 aacatcttag acagtgattc agaattactt gttgctggta aaggaaaaga aaaagtagaa
    14161 gaagctttcc aaacaacttt gaccaacaac cgtgctttgc ttaaaggtgt cgtttcacgt
    14221 aaaaaacaag tcgtacctca attgacggct gtatttagct aatcaaatgg tagtatgaag
    14281 cattcaaaat gaatggataa gcgattgata taaaaagaaa catagaacat actaaaggcc
    14341 gaagaaatgg aggagaataa cagtcaatta tgactcttgt tcttcctcct ttttacgtaa
    14401 aagcctttta agtaaaataa aacggataga aaagagaaca tatatgaact attattcaaa
    14461 aaataaggaa aacttttatc aaaaactgac cgatgatccg cttttttctt cattgaccga
    14521 ttatctatat gaacatcgag aacaagaaac gattctaaga gaattgaaaa aagaattttc
    14581 gcaaaataaa ttttcccatt ttcttgattt attgatcgat gcaggattaa tcaagaggga
    14641 agaacgaaga tatcatttga actttccaat atttgatcct aaagattact tgcaacaagc
    14701 cacttcggct gctgaaacca tagctgaaca attgaagaga ctctctgttg atgaacaaaa
    14761 attagcgatg ggagaagtta tttgggcgta ttgtttcgaa gatgaacgga aagaagcgta
    14821 tttttatgga gtaagaaaca gccgagagac agaattgctg aggacaactg caggaaatga
    14881 gaagtaccgt tttatcacac tttcctctat agaacacttc ccgcttactt tggctaatta
    14941 cttttttgta cagaagaatc agcttcctgt aacgaaggca ttcaaagaat tagccgaact
    15001 tattggagac gtaaatgaat cgtacttttt tgatcaaata gaagttatcg ttgacaggat
    15061 acgtaagaat aaatataaaa atcgccggcc tagtattttc catcagtctc tacttgtgac
    15121 gaatactata aaggaagaag aatcattcac attagaatta cctattgtcg aaaaaaacaa
    15181 ttttgagatt gaattaccaa cattggatcc ttcattgaca atggaggaaa tggcctttct
    15241 taaacgacaa atcttttctg agctgagcaa gaaatttata ccgcatgcat tttcttatat
    15301 caaagaatat ggaacagttt tagtgagtaa aacctaagag agcagtaagc aaaaattata
    15361 ctgactcttg caggaggagc ctttctcttc ctttatggtt aaaagaatta tttgagtatt
    15421 ttgcctatct accacctatt agattcacta catggtctgg aaacaaagga atgcaaaaaa
    15481 atggtgagaa atgtggaatg acaaaggaag tagtgataag aaaagttaga tttttaaata
    15541 atcaatatta tgattcagtc aagtatggca ttttatggga agaactagct gattagatta
    15601 ggaagtccat ttgataatcc agaatcacta agaaacgaat ggcgagtcat taataattta
    15661 tccaatcttg ggttattctt ttagattgaa gtgtttaaaa ctaaaaaagc atttatgaag
    15721 tcgtaatagt caggcttcaa aatgcttttt attttgtttg ttttttcttt gccacataaa
    15781 tacacctaga ctgacgatga agagcatatc caacagattt cctgcgactt ggatcgtcga
    15841 agggttggtc agtggcatta aagcaaggac aaaaaaggaa aacaagctgt aaataaaaca
    15901 ggtgacagga actaatcgaa agaagagcgg acatttcaat tggctaaaaa tgagtgaaaa
    15961 aataaaataa gtaaaaatgg ataaaatcac gactaaacca ttcatgataa agaagaaaag
    16021 cgtattgtga ttaaacgaat aaaaaagaat tgctaaaaaa gaaaacgttt taaatgcaat
    16081 caatacgctt gaagcttcgt ttaacgtacc aggatgtcga tgaaaaaaat aggcaccgaa
    16141 ataaacagaa aacagtaagc cgcaaagaga agacaaaatc aaaaaaaacg gattttcttc
    16201 ggcttctgtg atatcagaat gatccaaaag caaccagcct aatgcactag aaggcgcatg
    16261 tgaggtctta aggtatgaat aacatagaaa aaataagatc gtcaaagttg caaaaaaaga
    16321 gcctaaatgt aaaaaattag tggttctacg ttgaatttcc ataatatctc caaatta
//
LOCUS       DAYYHS010000065        16165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000065 DAYYHS010000000
VERSION     DAYYHS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.065"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(292..2019)
                     /locus_tag="ACHA6A_002061"
     CDS             complement(292..2019)
                     /locus_tag="ACHA6A_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8970231.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2012..3205)
                     /locus_tag="ACHA6A_002062"
     CDS             complement(2012..3205)
                     /locus_tag="ACHA6A_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8970232.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3392..4036)
                     /locus_tag="ACHA6A_002063"
     CDS             complement(3392..4036)
                     /locus_tag="ACHA6A_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8970233.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4130..4264)
                     /locus_tag="ACHA6A_002064"
     CDS             complement(4130..4264)
                     /locus_tag="ACHA6A_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8970234.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4266..6398)
                     /gene="feoB"
                     /locus_tag="ACHA6A_002065"
     CDS             complement(4266..6398)
                     /gene="feoB"
                     /locus_tag="ACHA6A_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8970235.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6395..6868)
                     /locus_tag="ACHA6A_002066"
     CDS             complement(6395..6868)
                     /locus_tag="ACHA6A_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8970236.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7269..7493
                     /gene="nrdH"
                     /locus_tag="ACHA6A_002067"
     CDS             7269..7493
                     /gene="nrdH"
                     /locus_tag="ACHA6A_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8970237.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7652..9811
                     /gene="nrdE"
                     /locus_tag="ACHA6A_002068"
     CDS             7652..9811
                     /gene="nrdE"
                     /locus_tag="ACHA6A_002068"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8970238.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9861..10826
                     /gene="nrdF"
                     /locus_tag="ACHA6A_002069"
     CDS             9861..10826
                     /gene="nrdF"
                     /locus_tag="ACHA6A_002069"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8970239.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10915..12054)
                     /locus_tag="ACHA6A_002070"
     CDS             complement(10915..12054)
                     /locus_tag="ACHA6A_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8970240.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12363..13076)
                     /locus_tag="ACHA6A_002071"
     CDS             complement(12363..13076)
                     /locus_tag="ACHA6A_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8970241.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13330..14031)
                     /gene="nagB"
                     /locus_tag="ACHA6A_002072"
     CDS             complement(13330..14031)
                     /gene="nagB"
                     /locus_tag="ACHA6A_002072"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8970242.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14265..15797
                     /locus_tag="ACHA6A_002073"
     CDS             14265..15797
                     /locus_tag="ACHA6A_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8970243.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15988..>16165)
                     /locus_tag="ACHA6A_002074"
     CDS             complement(15988..>16165)
                     /locus_tag="ACHA6A_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8970244.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5006 a         3340 c         2829 g         4990 t
ORIGIN      
        1 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
       61 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      121 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
      181 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
      241 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
      301 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
      361 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
      421 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
      481 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
      541 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
      601 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
      661 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
      721 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
      781 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
      841 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
      901 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
      961 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1021 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1081 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1141 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     1201 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     1261 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     1321 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     1381 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     1441 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     1501 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     1561 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     1621 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     1681 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     1741 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     1801 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     1861 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     1921 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     1981 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2041 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2101 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     2161 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     2221 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     2281 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     2341 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     2401 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     2461 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     2521 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     2581 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     2641 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     2701 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     2761 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     2821 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     2881 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     2941 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3001 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3061 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3121 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     3181 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     3241 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     3301 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     3361 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     3421 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     3481 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     3541 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     3601 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     3661 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     3721 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     3781 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     3841 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     3901 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     3961 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4021 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4081 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4141 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     4201 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     4261 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     4321 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     4381 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     4441 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     4501 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     4561 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     4621 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     4681 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     4741 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     4801 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     4861 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     4921 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     4981 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5041 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5101 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     5161 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     5221 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     5281 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     5341 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     5401 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     5461 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     5521 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     5581 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     5641 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     5701 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     5761 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     5821 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     5881 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     5941 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6001 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6061 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6121 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     6181 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     6241 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     6301 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     6361 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     6421 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     6481 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     6541 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     6601 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     6661 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     6721 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     6781 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     6841 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     6901 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     6961 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7021 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7081 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7141 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     7201 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     7261 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     7321 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     7381 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     7441 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     7501 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     7561 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     7621 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     7681 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     7741 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     7801 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     7861 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     7921 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     7981 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8041 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8101 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     8161 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     8221 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     8281 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     8341 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     8401 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     8461 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     8521 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     8581 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     8641 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     8701 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     8761 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     8821 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     8881 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     8941 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9001 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9061 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9121 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
     9181 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
     9241 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
     9301 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
     9361 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
     9421 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
     9481 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
     9541 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
     9601 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
     9661 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
     9721 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
     9781 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
     9841 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
     9901 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
     9961 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10021 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10081 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10141 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    10201 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    10261 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    10321 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    10381 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    10441 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    10501 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    10561 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    10621 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    10681 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    10741 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    10801 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    10861 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    10921 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    10981 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11041 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11101 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    11161 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    11221 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    11281 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    11341 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    11401 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    11461 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    11521 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    11581 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    11641 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    11701 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    11761 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    11821 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    11881 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    11941 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12001 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12061 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12121 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    12181 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    12241 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    12301 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    12361 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    12421 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    12481 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    12541 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    12601 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    12661 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    12721 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    12781 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    12841 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    12901 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    12961 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13021 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13081 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13141 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    13201 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    13261 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    13321 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    13381 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    13441 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    13501 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    13561 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    13621 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    13681 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    13741 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    13801 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    13861 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    13921 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    13981 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14041 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14101 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    14161 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    14221 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    14281 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    14341 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    14401 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    14461 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    14521 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    14581 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    14641 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    14701 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    14761 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    14821 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    14881 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    14941 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15001 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15061 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15121 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    15181 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    15241 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    15301 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    15361 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    15421 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    15481 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    15541 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    15601 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    15661 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    15721 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    15781 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    15841 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    15901 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    15961 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16021 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16081 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16141 aataaagtac ataaacataa ataca
//
LOCUS       DAYYHS010000066        15860 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000066 DAYYHS010000000
VERSION     DAYYHS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.066"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACHA6A_002075"
     CDS             complement(12..482)
                     /locus_tag="ACHA6A_002075"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8970245.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACHA6A_002076"
     CDS             complement(641..1099)
                     /locus_tag="ACHA6A_002076"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8970246.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACHA6A_002077"
     CDS             complement(1270..2415)
                     /locus_tag="ACHA6A_002077"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8970247.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACHA6A_002078"
     CDS             complement(2428..3345)
                     /locus_tag="ACHA6A_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8970248.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACHA6A_002079"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACHA6A_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8970249.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="ACHA6A_002080"
     CDS             4437..4607
                     /locus_tag="ACHA6A_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970250.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="ACHA6A_002081"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="ACHA6A_002081"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8970251.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="ACHA6A_002082"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="ACHA6A_002082"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8970252.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /gene="dapA"
                     /locus_tag="ACHA6A_002083"
     CDS             6871..7752
                     /gene="dapA"
                     /locus_tag="ACHA6A_002083"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8970253.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /gene="dapB"
                     /locus_tag="ACHA6A_002084"
     CDS             7749..8465
                     /gene="dapB"
                     /locus_tag="ACHA6A_002084"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8970254.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="ACHA6A_002085"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="ACHA6A_002085"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8970255.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="ACHA6A_002086"
     CDS             9182..10183
                     /locus_tag="ACHA6A_002086"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8970256.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="ACHA6A_002087"
     CDS             10180..11541
                     /locus_tag="ACHA6A_002087"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8970257.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="ACHA6A_002088"
     CDS             11501..12652
                     /locus_tag="ACHA6A_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8970258.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="ACHA6A_002089"
     CDS             12627..13796
                     /locus_tag="ACHA6A_002089"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8970259.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="ACHA6A_002090"
     CDS             13971..15350
                     /locus_tag="ACHA6A_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8970260.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..15860
                     /locus_tag="ACHA6A_002091"
                     /pseudo
     CDS             15631..15860
                     /locus_tag="ACHA6A_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5185 a         2799 c         3378 g         4498 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt
//
LOCUS       DAYYHS010000067        15190 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000067 DAYYHS010000000
VERSION     DAYYHS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.067"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHA6A_002092"
     CDS             complement(93..1136)
                     /locus_tag="ACHA6A_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8970261.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="ACHA6A_002093"
     CDS             complement(1513..2478)
                     /locus_tag="ACHA6A_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8970262.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="ACHA6A_002094"
     CDS             2712..3557
                     /locus_tag="ACHA6A_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8970263.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="ACHA6A_002095"
     CDS             3748..4935
                     /locus_tag="ACHA6A_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8970264.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="ACHA6A_002096"
     CDS             4941..5297
                     /locus_tag="ACHA6A_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8970265.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="ACHA6A_002097"
     CDS             5299..5637
                     /locus_tag="ACHA6A_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8970266.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="ACHA6A_002098"
     CDS             6032..7066
                     /locus_tag="ACHA6A_002098"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8970267.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="ACHA6A_002099"
     CDS             7059..7745
                     /locus_tag="ACHA6A_002099"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8970268.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="ACHA6A_002100"
     CDS             7769..8617
                     /locus_tag="ACHA6A_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8970269.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="ACHA6A_002101"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="ACHA6A_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8970270.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="ACHA6A_002102"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="ACHA6A_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8970271.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="ACHA6A_002103"
     CDS             10883..12118
                     /locus_tag="ACHA6A_002103"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8970272.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /gene="sufU"
                     /locus_tag="ACHA6A_002104"
     CDS             12105..12575
                     /gene="sufU"
                     /locus_tag="ACHA6A_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8970273.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="ACHA6A_002105"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="ACHA6A_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8970274.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..>15190)
                     /locus_tag="ACHA6A_002106"
     CDS             complement(14069..>15190)
                     /locus_tag="ACHA6A_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8970275.1"
                     /translation="KLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAEAQTALNR
                     LLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPAFGELRL
                     DKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLLPRKIEE
                     EQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDIDYFNKT
                     ITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMILYGYNTN
                     SPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFESGASIK
                     EVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4964 a         2835 c         3249 g         4142 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt
//
LOCUS       DAYYHS010000068        14257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000068 DAYYHS010000000
VERSION     DAYYHS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.068"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(433..1617)
                     /locus_tag="ACHA6A_002107"
     CDS             complement(433..1617)
                     /locus_tag="ACHA6A_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8970276.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1642..2649)
                     /locus_tag="ACHA6A_002108"
     CDS             complement(1642..2649)
                     /locus_tag="ACHA6A_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8970277.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2821..3180)
                     /gene="comGG"
                     /locus_tag="ACHA6A_002109"
     CDS             complement(2821..3180)
                     /gene="comGG"
                     /locus_tag="ACHA6A_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8970278.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3152..3577)
                     /gene="comGF"
                     /locus_tag="ACHA6A_002110"
     CDS             complement(3152..3577)
                     /gene="comGF"
                     /locus_tag="ACHA6A_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8970279.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3540..3887)
                     /locus_tag="ACHA6A_002111"
     CDS             complement(3540..3887)
                     /locus_tag="ACHA6A_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970280.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(3859..4329)
                     /gene="comGD"
                     /locus_tag="ACHA6A_002112"
     CDS             complement(3859..4329)
                     /gene="comGD"
                     /locus_tag="ACHA6A_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8970281.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4304..4582)
                     /gene="comGC"
                     /locus_tag="ACHA6A_002113"
     CDS             complement(4304..4582)
                     /gene="comGC"
                     /locus_tag="ACHA6A_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8970282.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4579..5628)
                     /gene="comGB"
                     /locus_tag="ACHA6A_002114"
     CDS             complement(4579..5628)
                     /gene="comGB"
                     /locus_tag="ACHA6A_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8970283.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5585..6514)
                     /gene="comGA"
                     /locus_tag="ACHA6A_002115"
     CDS             complement(5585..6514)
                     /gene="comGA"
                     /locus_tag="ACHA6A_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8970284.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6646..8400
                     /gene="ade"
                     /locus_tag="ACHA6A_002116"
     CDS             6646..8400
                     /gene="ade"
                     /locus_tag="ACHA6A_002116"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8970285.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8498..9160)
                     /gene="trmB"
                     /locus_tag="ACHA6A_002117"
     CDS             complement(8498..9160)
                     /gene="trmB"
                     /locus_tag="ACHA6A_002117"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8970286.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9230..10012)
                     /locus_tag="ACHA6A_002118"
     CDS             complement(9230..10012)
                     /locus_tag="ACHA6A_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8970287.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10216..11427)
                     /locus_tag="ACHA6A_002119"
     CDS             complement(10216..11427)
                     /locus_tag="ACHA6A_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8970288.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11420..12157)
                     /locus_tag="ACHA6A_002120"
     CDS             complement(11420..12157)
                     /locus_tag="ACHA6A_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970289.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12295..12729
                     /locus_tag="ACHA6A_002121"
     CDS             12295..12729
                     /locus_tag="ACHA6A_002121"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8970290.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12722..13066
                     /locus_tag="ACHA6A_002122"
     CDS             12722..13066
                     /locus_tag="ACHA6A_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8970291.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13246..14256
                     /locus_tag="ACHA6A_002123"
     CDS             13246..14256
                     /locus_tag="ACHA6A_002123"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8970292.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4533 a         2910 c         2401 g         4413 t
ORIGIN      
        1 ttttttcgca acaaaaaact tgattttcat acctgtgctt tcacgattca ggcaaagtac
       61 aggtggaaaa tcaagttttt attattcgat caatgaatgt ttcggatcgg ttttctagct
      121 aaacataaag cgaagaataa agtcgacagc ccacgaggct gatctaaaaa tgttttttct
      181 ttttacatat gtaatgtccc ttataaagat gaatgaaaat aaaatattgg gattttcctg
      241 aaaatcaata aataaaatgc cagtcttttc ccttttgacc acttttccaa gcaaaattcc
      301 ctaagaagat cctgtaaaaa agaagggaaa ttgaagttaa attaccattt tgcggtcttc
      361 tccgcaaaaa aaacacatgc aagaattccc tcttgcatgc gtttcatctt ttatcctaac
      421 gaatgctttg gcttagccgc gcagacgctc cacatcacgg gcaatcatta gttcctcatc
      481 tgttgggatc actaatactc ttacttttga ttcttttgta gagatatcta cttcttcacc
      541 acgcaattca ttcaacgctg gatcgatttc acaaccaaac caagacatac ctttgatgat
      601 ttcactacgt acatgtccgt cattctcgcc gataccagca gtaaagacaa ttgcatcaac
      661 accattcatc gttgtcacat aaccgccgat gtattttcgg atgcggtcca cgaaaatgtc
      721 ataagctaaa cgaatatgtt ctttttcata gttcttttca agatcacgca tatcactaga
      781 caatcctgtt aggcctagca gaccagattt cttgttcaaa acattgatca tttcatcaat
      841 atcaatctct aatttttcca ttaaatatgg gagaacagct ggatcgatat cacctgaacg
      901 tgtacccatt gtcaccccag ctaatggtgt gaatcccata gaagtatcca tggatttacc
      961 tccatcgact gctgtgatcg atacaccatt tcctaagtgg caagtgatga tttttagttc
     1021 ttcgatcggt ttgttcaata attttgccgc acgttccgac acataacgat gacttgtgcc
     1081 atggaagcca tattttctta ctttgaaatc ttcataataa gacaccggta agctatataa
     1141 ataattttgc tttggcattg ttgagtggaa agatgtatcg aatacagcca cactcaaaat
     1201 gtcaggtaaa atctttttaa acgctttgat ccccattaag tttgcaggat tatgcaaagg
     1261 agcaaattct gctaattcag caatgcggtc cattactttc tcatcgatca ctactgaatc
     1321 cccataagtt tctcctccgt gaacaacacg gtgccctact ccggtgattt catcataaga
     1381 gcttaaaatt tgcagttcaa tcaactgatc taacagcatt tttactgcta cttcgtggtt
     1441 ttcgatatcc atgatttgtt cgaatttttg attatcgccg tacttgatgg taaaaataga
     1501 atcttgtaaa ccgatacgtt cgacgatccc tttcgcaact acggtttctt ctggcatttg
     1561 atataattgc catttcaaac ttgaactccc agcattgatt gcaattgttt tagacatatg
     1621 actctccttc tcgttgaatg attacagagc agatgatttc cagtttttaa actcgttgaa
     1681 aaattccgtc accttctctg gttcttttaa tgaagcaagt ttcacaagca agacttcttt
     1741 tacttgttgt gcttgtggtc cttttttctg taagatcaaa atactttttt gcgattgttt
     1801 attacggaac aattcatctg gcaattgaat cattccttga agataacctt cttctttaaa
     1861 ccattttttg atttcttcac tttgatccgt ttctaaaaat ccactcggca tcaaaaacaa
     1921 accaaaacca tccggcttca catatttcat tgattgctct agcaataaat gatgtgcata
     1981 actatggcct tcttcggtac ttgttaaaaa ctcttttgct ttttcgtcat tcggataata
     2041 accgattggc aaatcgctga ttgcaaaatc gacaggatct attaataatt cctgcaaccc
     2101 gtcttgatga aagtattgaa catttgcttg tgtcaaatca cttgtagaag ctgcgacagc
     2161 caacaatgta tcatcaatat ccacaccaat tccttctgtt tggtaccccg cattcgatag
     2221 gtttaaaaga actgttaata acaagttgcc cattcctgcc gcaatatcca gaatcttaac
     2281 cggtgctttt ttatttgtgt atagttgttc aataagaaaa acaaataaaa aaccgatact
     2341 atcaggagtg agttgatggt tcgcctgcaa atgttctgtc tggctgccct tcaataaaag
     2401 taattgagac agtctgcgcc attcttctgg ctcgaaagaa agtttcttca actcttcata
     2461 aagagctgtg atccgttgcg ttgtctcttt tgtcggcact ccatccacca cacgtacttg
     2521 ataatcatct attaaatttt ctgcattttc gacataggct tctaaaaagg aagttcccaa
     2581 tgcattctgt aaaagctgaa tagcttccag gttttgatta aacccttgtt ccattttttc
     2641 tggtaacatg gtgcacctca tttctagata tattataaca attttttcac ctgtttaatt
     2701 ctatcgaaca attgacaaaa tttcaatcgt tttcactgtt ttttatttgt gaaacggcaa
     2761 acattttttt attaaaaaca gtgttataac agaaaagagg gcgttttcac gaacattcca
     2821 tcattctgta ctagtatctg tatcattttt tacttcttct gtgaaccgaa agataaacgg
     2881 tgagacttcc acctctaaga atagggactg ttgtacccac tgatatgtta cagatccagt
     2941 tgtgtagtaa aaaactcctt ccgaactttc ggcaagcgta gactgttccg ataaagccat
     3001 ctgcttcatt atttttgcta tataataatt tttgattcgt atattaagat ccattgacaa
     3061 acggtagctt tctaatgctg ccatcaataa aaacgtacat aaaagaaata aaagcaagac
     3121 agaaaataaa atagagccct tataacattt tctactgatc gttttttgtc catatggcat
     3181 atccatcttc tccattttca aagtgtacat aaaaagaaat tgtgtgttct ttttccaata
     3241 attgaagctc agtgatactt gttaacatag gttgatgtcc gccggaagtt cttcttttac
     3301 gtatcattgc ctgatatctt tcgatgatat actgtttatc ctcgatcgtg tagtgcagtt
     3361 gctgattttt cacagagaca ttgtgactgc tcttgagttc ttcttctaat tgtgctaaaa
     3421 atattagcca ctctttttga tctttacgct gcaaaaattc acttacttgg atggtttgct
     3481 gaatcaaaag attgaatgtg agcaatagac tgctcaatat catcagtcca attagacatt
     3541 caatcagcgt aaatgctgga attacttgtt ttttcaatac tgatcgcttc tttctccata
     3601 ttgacaaaca ctttttcgat tactccatta ttatcgacaa tttggatatc gtatgggatc
     3661 atttcttccc ttcgctctgg tcttttgtga tacatccgct gcgtcttgat atcttcataa
     3721 atcattcgaa taaatagcag ttcctcccca cttttctttt ctgcctgaag caaaaacagc
     3781 tggccaaagc tcactaagta gacacagaca gaagcgatca taagcgctgt aagggactcc
     3841 aacaaaacaa atcctgtttt attcaattct ttttgtatag cgaccactcc ctatctggaa
     3901 ttgatacgta atcgtccgct ctttttcttc ccaataaaat tgataagctt ttagtgaact
     3961 ttcattcccg gttctagcag caaaagtaat agaagcatgg cgtttcaagg taatctcatc
     4021 aggaatatct aatacttccc aatttatttt atctggattc ggaacatgaa aaatgatttg
     4081 attctcttca ctattccaat aaaagcctgt ttggatctga ttcacaatag cgattttttg
     4141 cgtagaatag attcgttttt caaattggcc gaaaaattga tagactgcca attcattctt
     4201 ccatgcagaa aatgcgagaa ctggaaaaga aaggatcgtt gtaagtaata aaatcagtaa
     4261 gagtgattca aacaacgtat atcctgatgc taaaacctta ccttcaaggc cgctgataga
     4321 tttctgcatg ttctcgtttg atatagcctt cattcaacaa ttcctccaca gtaggaactc
     4381 ttccattatt ttgaagcgca tagagctctt tttgtgtgtc taccaactgg atgatcgctt
     4441 cacgactttc ttggtctaca tgattcctat aacgcgacaa attagggacg aacaacaaca
     4501 ctaatacact gatgatcagt aagacgatta gcatttcgat caatgtaaaa ccgctataat
     4561 aagatatttt tctctttttc atggcaaaac ctcctctatg cttccgtaaa caggtaataa
     4621 aacagcggca taaactaata aaatcaaagc cgctacgccc aaaaaaatga ttggctgaat
     4681 aaaatgcatc cagtgatgaa gacgtttgaa aagctgtttt cttgtccatt cgctgtaaaa
     4741 aatcagttct ttacctaaac ttccctttgc ctctccctgt aaaacaattt tactgaattc
     4801 ttctgataga aaacgataat tctggaactg ctcagccaat gacatacctg cctccaatcc
     4861 ttgaatcaac ttagaggcaa gatgctgaat cagactttct tgtttcgttt cctttaagca
     4921 gactatgatt tgccttaatt cgaaaccttc atgaaaaagc tttccaatct ctagtgacaa
     4981 ataagccgat tgatacaagg aatatatcgg tccaatcaat aattgtctgc ttatccattc
     5041 actctgatca acaggagaca acttcatcag acgatacctt actagtaaag ccaatagagt
     5101 aaccgctagg atgaatagta gaacatacca atgaaagact tgtatcagct gaatacccca
     5161 atgctcttgc atgaccattc ctgaaccaat aagctgaggc aaaacgaaca accgcatact
     5221 aaacaagata cctagcaaaa aggtgagcaa tagcatcgga tatgcaagca gctttttcaa
     5281 atcattgcga aatccttcaa caagccgaaa ctgtttggca atttccaaca aagttgtcgg
     5341 cagattccca tgacgttcag ctaactcgat ctgtaccagt tgctctgctc catatcctat
     5401 ttgcgaaagt acctcactaa acgcttgccc ttcctttagc tgatgttgga tatttgccaa
     5461 aataacaggt ggaaacgaac ggatcgtgag taataaataa agggattctt gcagactaaa
     5521 accagataaa agcagttctc ccatggtttg tgcaaactgg ttttgctggc ttcttgttaa
     5581 tcctttagaa tagttctgac tgccaaatat tttcctgagc ttcttcataa ctttcctccc
     5641 atgatttttt tactgattct tcctccataa attgataact ccataaaaca gaaggatggt
     5701 gattggcatc ttccaaaagt tcttgataaa tgacaccagc taaacattca gatagctctt
     5761 cttttttctt tgtcagttca tggattctga ccaatgtacc ttcaagattg cgtgcatgta
     5821 cggttgcaaa aacccggtgc cctgttagtg ctgctcttac cgcagcatgt gcagttgatt
     5881 catctcttat ctcgccaatg attagcaagt ctggacgatg ccgtaatgcg agtctcaaaa
     5941 gtgtatcata agtctgtgct attttcggat tgatctgtag ctgcagaaaa aacggttctt
     6001 ctatctctac aggatcttcg atagtaatca cttgcaattt ttcttccaaa gccaaccgat
     6061 acattagaga agtcttgcct gatccgactg gtccgctgaa aaggtataat ccacgtctga
     6121 cggttttctg taaaatagtt gcaaaatctc gagaactaaa gcaacgatgg ggtgtttcaa
     6181 aagtaccgta taatagccga atcactaaac tctctcgttg aaggtaatct cctacagaag
     6241 acaaccgcag acgaacttcc tgtttttcta aagaataagt aaatgcacct aactgagctt
     6301 ttcttttttc tccgatatcc atctgtccca aatacttaaa tcttgagatc agttgctgtc
     6361 catctgccga agagactttc ttccagaatg ttcgtttttt tccatagcga aaatagatgt
     6421 aaaatacttc tgaagctggc aatatatata cgtcttgggc atgtttggca aaactttcac
     6481 ttatcatttg atccgataat cgttttattt ccattattct cccctccaca aaaaacatac
     6541 gtctttttga tctttttgtt tcaaataaaa tgaatttaca aaaaaatatt ggcaaaaacc
     6601 gaatttatgt tatacttata aaaatttatc ttgggaggca ctccattgga aacaacatta
     6661 aggaaatata tcgaagtagc aagtaaaaaa aagcctgctg acctagtgat caagcatgct
     6721 aagatcgtca acgtctttac gaaagaaatc atggaaaaag atgtagcaat ctgcgaagga
     6781 atgatcgtag gaatcggtga atacgaagga aaaagcgtac ataatgctaa tgggcaatat
     6841 ctagtgccag gattcatcga tggccatgtg catatcgaaa gctcactttt atcaccaagc
     6901 gaattttcaa aagtatcctt gcttcatgga gttactactg tagtgacaga tccccatgaa
     6961 ataggaaaca ttgctgggag tacaggtctt gagtttatga tagaagatgc aagacaaaca
     7021 ctcatgaata tcttttttat gctaccttct tgtgtacctg taaccccttt tgagaccaat
     7081 ggtgcaaatt taaatgcaaa aaaattagcc ccttttttac aaaaacccga agttttaggc
     7141 ttagctgaag tcatgaacta tcaagcagta gcaaataatg aaacagacat cctagaaaag
     7201 atccaattga tgcaccagca taagaaaaaa attgacgggc atgcagctgg tatcggcatg
     7261 gaagagttaa atgtttatcc tgcagcaggt attcggacgg atcatgaagc tacaactgca
     7321 aaagaagcta aagaacgttt ggatcttggt atgtatttaa tggttcgaga agggaccgtt
     7381 gcaaaagatc tggcatcgct actaccggcg atcacacctg aaaattcaag aagatgcttc
     7441 tttgtcaccg atgataaact gatcaatgat ttagtaaaag aaggatcaat cgatcatatc
     7501 gtccgtcaag ctattgcact tggaatcgat ccattacaag ccattcagat ggcaacattg
     7561 aatgcagcag aatgttttga tttgaaaaca ctaggtgcga ttgcccctgg gtatcaagct
     7621 gatttcttct taacagacga tcttaccaag ctgccaattc atgaagtttt ctataaggga
     7681 acattagttg ttcaatctgg gcaactccaa gaccatttat ttgctgatca ttcaaattca
     7741 tatacgtgtt gtctgccaaa actaaacgct caaccattaa atccacattc gttagagctg
     7801 cttctttctt cttcacaagc ccatgtgatc gaaattatcc cgaatagtct agtgactaaa
     7861 gatttggtag aaaaagtgga ccgacaaggc gaacgattca ttccttcaat cgaaaaagac
     7921 caattaaaga ttgcggtgat cgagcgtcac catcaaacgg gaaacattgg attaggcata
     7981 gtgaaaggat ttcagctgaa acaaggagcg atagcgacaa cgattgccca tgactcgcat
     8041 aatctagttg tcgttgggac aaacgacgag gatatgctgt atgctgctaa tcttttgatt
     8101 caaaaaggtg gcgggatgac cgttgtcaac aatcagcagg aaatagcttg tgtttctctt
     8161 cctataggcg gaatcatgac acaggaacct ttttcgatcg tctatgaaca gttgaatgaa
     8221 ttaataacta aagcacatca actaggagca gctaaaacct ttgatccttt tttgacactt
     8281 tctttcttaa cattatctgt catacccgaa ttgaagatca ccgacaaagg attattttca
     8341 ttttctcgct tccaattgat tccaccttgt ggaaaaaccg atgaaataaa tacattttaa
     8401 atcaaataaa tcccagaaaa tacattacat ctgtaatgtt cattttctgg gatttattta
     8461 tggatattaa tgtttttatg aaagcagaaa gttagtttca cagacttttc tttttggtct
     8521 tatcttgaaa ttgagcttct actcgataaa tacgttgacc ttttgaagaa aatttttctt
     8581 catactccgt cataatattg ccttcaaatt gactttgatg taaatctaac catacctgtt
     8641 tgaggatcat gccatattga gaaaaacttg ccagagaata ctcaaacaat ccttgattat
     8701 ctgttttgaa atggatttct ccatttggac gaagaatctg ttcatctaca gcaagaaaag
     8761 tcttatatgt caatcgccgt ttttcatgac gtttttttgg ccatggatcg gaaaaattca
     8821 gatagatttg atctacttcg ctgtcagcga aatactctgt caacgctgat ccatccacat
     8881 gtagaagttt caggttagga agcggttgtt cgatcaactt gtctagtgca atcgaaacaa
     8941 cactgacctg catttctact ccaatataat tgatttcagg atgggctttt gccattccag
     9001 tgatgaactg gcctttcccc ataccaatct caatgtggat tggatgatcg ttttcaaaca
     9061 attcattcca ttttcccttc cacaatgttg gatcactaat tacaaaattc gggtgagccg
     9121 ctaacatttc cgctgctccc ggacgatttc ggacacgcat tatttatctc cattcctttc
     9181 ctctaattaa aaaggtctgg ccaaatctcc gcagagtttg ggccaaacct cagccactga
     9241 aaattcgttt taattgtaaa atttcttcgt tcatccgtcg atgatcttca cgtttgcaat
     9301 atctggtgat ttcttggagc aaattcatgg ccgcatacca atgaaatttt tcaagcattt
     9361 cgtcagtcgg gcgtatgcca taactgatca gccactgatt ccagctagac agtggcacat
     9421 aatttcctaa aatcgttgca atatccaccg cagggtctgc aaacatgatt gaatcccaat
     9481 cgactagata taaataattt ttacatacta accaattacg atggtttaca tctccgtgta
     9541 ctactgataa gttttcctta tctattgacg ggatctgctc acgaagaaaa cgatggacaa
     9601 tttgcagaaa ttgatttttc tggagttctt ccggtaattc tttttcatat ttttgcagca
     9661 tcttctccgg cgtcatgaca cgaccaccga tacgggtcag catggtttta agagaatttg
     9721 aatgatgtaa atggtacaaa acatcaatca cgtcgtttct ttgaccaatc tcattaggag
     9781 tcagcaagcg accttccagc cactcttgtg ctgtcaaagt atcgccattc cccgttcgtt
     9841 ttgtccaaac aagttttggt gtaattcctt cccttgaaag agcagccaac atcggagtcg
     9901 tgtttcgttt aataaagact ttgtcctcat cacgcatgcc tatgtatgtt tttcctgtgt
     9961 cgcctttgat gggacgtaac cgccactctt ggtctaactg aaactccatc atgcattctc
    10021 ccgtcctctt actctttgtt ttgcagtact tattattcta gtcgcctgaa cactacacgt
    10081 caagcacata tacgttcttt ttcatttaat tgccattcaa aaaaccagct tttctcaaaa
    10141 aaaatagaac gaacagcatt cttactgtca acgctctatt tttcattctc atttttaccc
    10201 tacacagctt tgttttcagt ctgacatctt cttcaaacga gttggtacat atagtttaac
    10261 aaagagcata gtaacaatca aataagccgc tagcggaacc catcgctgct gcccatctaa
    10321 tacgactgca gcaatcaaac caaaaataac cgacgcgata attaataccc atgtgagtag
    10381 atactgaatc gcctgcttct tctgattctt atggattgga taaagctgaa cgagaaccat
    10441 atagcggaac tggttcgcta aaggtaacag ttggaaaccg atcaaatata aaaataagaa
    10501 accgataatc agactaaaat aaaagtcgac tactccggca atcaataacc cacctataat
    10561 tactaatcgg aaataaagac cactaaaatc agaacctctt gccaatcttc ttgcaaacaa
    10621 gtaaagataa gtgttttttg attctctttt aatatgagtc aatataggat ccaaatagcg
    10681 tcttcttttg atctttgccg taatttcagg aacatctgtg aataaattga taaattgata
    10741 gatctgatgc aacctatgtt cttctacatg caccattttc tcccaatcca atcctttttc
    10801 ttgttgcttc cacaaaaacc agtaatatag acttgcctgt actaatgcac ctgctattcc
    10861 tgcccatatt agccaataaa atgatagaaa caaaaagatc agagaagaaa aaaaccagcc
    10921 taatttccaa taacgaataa cttgttgatc tagtcgaaac aattctgctc tcttgatttg
    10981 cagatgactg aatttcaaac tccataatat gatcagatag acgaaaaaca tcgaaaagga
    11041 ttgctccgta ctaaccacca ctaatggcat ggccaatccg catgccaata tcaaaaagat
    11101 caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa taggtttgca tttctttttc
    11161 tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa gtagccatct tacccacgaa
    11221 taaacagagc atccaaaata cagagattat gagccctcca attgcaaacg gtggctgcag
    11281 ggttttcaac caattagaat aatataatcc cacaccgcca atcaagaaaa gacaaactaa
    11341 aacaaaatga tcgttcaata cataacgcat atatttcatc attttctttt gatgtcttgc
    11401 taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt tgttaaagca atatagattt
    11461 cgtccaaaga agaatccggc agctgaaatt cttttctcaa catctcaaga gaaccgtctg
    11521 ctcgtatctc tccgttatgt aagaccacaa aacgatcaca atatttttct gcagtagcta
    11581 aaatatgcgt agacatcaaa ataccggctc cttctttttt catttctacc atcaattcaa
    11641 gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg ttcgtcgatg atatataaag
    11701 aaggctcgat caaaaaggcg cataagatca tcactttctg tttcattccc tttgaaaaat
    11761 tagctgggaa ccagtcaaga cgattagata accgaaaagt ctccaacaac ttttctgctc
    11821 ttttcattgc tatatccata ggtatatcgt aggccatcgc agtgacttcg atatgttctc
    11881 tcaatgtcaa ttcttcatat aaagaaggag tctctggaat atacccaatt tgtttgcgat
    11941 attcttccgt atttttcttt aaggatagat gattgatttc tatcgttcct tttgaagctt
    12001 ccagcagacc gataatgtgc ttgatggtcg tacttttccc tgctccgttc agtccaatca
    12061 accctactat ctctccaggc tgtacatcga aactgatatc ttttaacaca gggagatgtc
    12121 cgtacccccc tgtgacatga tcgataatta aactcattct ttttcctccg ccatttattt
    12181 ttatttatta taccacgaag aaaatgacgg tattttgttt ttttccttac aatagcttta
    12241 acttatggta aagttaagct gaagtattta ttctttatag aaaggatgtt ttatatggaa
    12301 aactgtattt tttgcaaaat catcaatcaa gaaatcccaa gctataaaat atatgaagac
    12361 gacaaggtct atgctttttt agacatctct caagcaacga aaggacacac attagttgtg
    12421 cctaaacgtc atgtagcaga tatttttgaa tatgatcccg aacttgcagg agaggttttt
    12481 tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt ttccagaaat gaaaggtctt
    12541 aatatcttga ataacaaccg tgaactagcc tatcaatctg tttttcattc acatatccat
    12601 ttagtccctc gctacagcaa agaggatgat ttttctattc actttgtcaa tcatcaagat
    12661 agttatggat cggaagaact aaaagctatt caagaaacaa tcgtaaagca ggtgtcctgc
    12721 gatgattaaa aactttttca aaggactgtt atttggagct gctgctggca ctgtcggagg
    12781 tctgttattg gcgcccagga gcgggaatga aacaagaaag aaactgatcg aagaattgga
    12841 agaagcaaca actctgacaa atgatttaaa taacagcctg aatcatttta aaagcgcatt
    12901 gatcgaaaca aaagaaacag ctgaatctgt gattcctgct ttccaagaag caatcaaaaa
    12961 agatatcgaa gattttaaat tccaagccga accacgtatc acacaaatca atgaacaagt
    13021 agaaaaacta aaagctgatc tgccagagta tcctgaacaa atataataat gattattttc
    13081 tcaacagatt taactatata agtgagaaaa gtttgcgtat ttcctaggaa tgtgatgaaa
    13141 ttttgaattt tgtttgaaac taccacgaca acgtttttaa aggaaatggg atatggtata
    13201 gttaatatat aaaaataata actaattaca ggagtgcaaa ttttaatgaa aaaaaaatca
    13261 attattttgg cagctactag tgcgttggct gttttaactc tagcagcttg ttcaggcgat
    13321 actaataaag atatcgcaac gatgaaaggc ggaacgatca ctgtatctga tttctatgat
    13381 gaagcaaaac ttgaatcatc gaaccaatcc ttagtacaac ggatgatcat ttataaagta
    13441 ttcaataaca aatatggcga taaagtaacc gataagcaag tggatgcaga atacgacaaa
    13501 caagcgaaat cattaggtga tacttttgaa tcacaattag aagctgctgg atatacgaaa
    13561 gatacttata aagaatatat tcgtaacaat ttagcttttg aagctggctt gaaagctcac
    13621 gtagatatta cagatgacga tttgaaaact gcttggaaat ctttccatcc agaagtagaa
    13681 gcacaaatca tcaaattatc aagtgaagat gaagcaaaag atgtgaaaaa atcagcggat
    13741 gatggagatg atttctctaa attagccaaa gacaaatcaa ctgatacaga gacaaaagaa
    13801 gatggcggta aagttaaatt cgactcaacg acaacaacta ttcctgcaga agtaaaagaa
    13861 gcagctttca aattgaaaga cggtgagatc tccgatgtta ttactacaac aaatcctact
    13921 tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa accaaaataa aggcaatgac
    13981 atggataaat acaaagatca actaaaagat atcgctactg aaactaaact aagtgacaat
    14041 gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg caaatgtaaa aatcaaagat
    14101 gatgcctttg aaaatgtgtt gagtgcattc acaacaactt catcaagtac gaaagactca
    14161 agtgaaacta cagcttcaac aaagagttct gacacaaaat caactgattc aacaaaagaa
    14221 tcttcaactg aagaaacaac tgattcatca aaataaa
//
LOCUS       DAYYHS010000069        14192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000069 DAYYHS010000000
VERSION     DAYYHS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.069"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHA6A_002124"
     CDS             complement(<1..1835)
                     /locus_tag="ACHA6A_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8970293.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHA6A_002125"
     CDS             complement(1832..2536)
                     /locus_tag="ACHA6A_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8970294.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHA6A_002126"
     CDS             complement(2548..4857)
                     /locus_tag="ACHA6A_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8970295.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHA6A_002127"
     CDS             complement(5052..5516)
                     /locus_tag="ACHA6A_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970296.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHA6A_002128"
     CDS             complement(5516..6142)
                     /locus_tag="ACHA6A_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8970297.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHA6A_002129"
     CDS             complement(6149..6514)
                     /locus_tag="ACHA6A_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8970298.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHA6A_002130"
     CDS             complement(6504..6842)
                     /locus_tag="ACHA6A_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970299.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHA6A_002131"
     CDS             complement(6832..7158)
                     /locus_tag="ACHA6A_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8970300.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHA6A_002132"
     CDS             complement(7139..7417)
                     /locus_tag="ACHA6A_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8970301.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHA6A_002133"
     CDS             complement(7419..8783)
                     /locus_tag="ACHA6A_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8970302.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHA6A_002134"
     CDS             complement(8796..9365)
                     /locus_tag="ACHA6A_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8970303.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHA6A_002135"
     CDS             complement(9337..10524)
                     /locus_tag="ACHA6A_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8970304.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHA6A_002136"
     CDS             complement(10588..12315)
                     /locus_tag="ACHA6A_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8970305.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHA6A_002137"
     CDS             complement(12312..12764)
                     /locus_tag="ACHA6A_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8970306.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHA6A_002138"
     CDS             complement(12876..13256)
                     /locus_tag="ACHA6A_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8970307.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHA6A_002139"
     CDS             complement(13253..13639)
                     /locus_tag="ACHA6A_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8970308.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHA6A_002140"
     CDS             complement(13641..13919)
                     /locus_tag="ACHA6A_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970309.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13982..>14192
                     /locus_tag="ACHA6A_002141"
     CDS             13982..>14192
                     /locus_tag="ACHA6A_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970310.1"
                     /translation="MLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGVSSYSA
                     FDNDRIFGVLVQCINDECNKFYSLQF"
BASE COUNT         3925 a         3056 c         2233 g         4978 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt ta
//
LOCUS       DAYYHS010000070        14130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000070 DAYYHS010000000
VERSION     DAYYHS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.070"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACHA6A_002142"
     CDS             complement(<1..244)
                     /locus_tag="ACHA6A_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8970311.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="ACHA6A_002143"
     CDS             complement(237..1829)
                     /locus_tag="ACHA6A_002143"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8970312.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="ACHA6A_002144"
     CDS             complement(2339..3064)
                     /locus_tag="ACHA6A_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970313.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="ACHA6A_002145"
     CDS             complement(3218..3544)
                     /locus_tag="ACHA6A_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970314.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="ACHA6A_002146"
     CDS             complement(3581..3946)
                     /locus_tag="ACHA6A_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8970315.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="ACHA6A_002147"
     CDS             complement(3983..4198)
                     /locus_tag="ACHA6A_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8970316.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="ACHA6A_002148"
     CDS             4327..4662
                     /locus_tag="ACHA6A_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8970317.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="ACHA6A_002149"
     CDS             complement(4738..6735)
                     /locus_tag="ACHA6A_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970318.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="ACHA6A_002150"
     CDS             complement(6833..7108)
                     /locus_tag="ACHA6A_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970319.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="ACHA6A_002151"
     CDS             7340..8074
                     /locus_tag="ACHA6A_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8970320.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..9255
                     /locus_tag="ACHA6A_002152"
     CDS             8113..9255
                     /locus_tag="ACHA6A_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8970321.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(9883..10368)
                     /locus_tag="ACHA6A_002153"
     CDS             complement(9883..10368)
                     /locus_tag="ACHA6A_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8970322.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(10370..11041)
                     /locus_tag="ACHA6A_002154"
     CDS             complement(10370..11041)
                     /locus_tag="ACHA6A_002154"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970323.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(11304..12497)
                     /locus_tag="ACHA6A_002155"
     CDS             complement(11304..12497)
                     /locus_tag="ACHA6A_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8970324.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(12581..12781)
                     /locus_tag="ACHA6A_002156"
     CDS             complement(12581..12781)
                     /locus_tag="ACHA6A_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8970325.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(13205..13438)
                     /locus_tag="ACHA6A_002157"
     CDS             complement(13205..13438)
                     /locus_tag="ACHA6A_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970326.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(13445..13876)
                     /locus_tag="ACHA6A_002158"
     CDS             complement(13445..13876)
                     /locus_tag="ACHA6A_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8970327.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4295 a         2895 c         2270 g         4670 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     8821 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     8881 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     8941 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     9001 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     9061 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     9121 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     9181 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     9241 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     9301 aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac
     9361 tgccttccaa tgactctttt gggaatcaga ggaaagaatc aggctttata gagatatttc
     9421 atctaacaaa ttactttaga ctgacagtat atatcagcag gtactgtcat cgggaacgta
     9481 gccaatatcg tatccaaaac agcaggtaga gaagctattt gaggattcaa gttattctat
     9541 ttcaaccatg aagttaagtt cattaaaaat aaagatagtt ggtatttcaa taatttttgt
     9601 atagagttaa tcactttatt gatgctaatt catcttgatt ggtattataa tcgtattcaa
     9661 gttgcgtggc gtgcttttgc tagaaaatat ataactgttc tttataaaga aatataagga
     9721 ttaaacattt tttatcatag agttttactt atctatagaa ttatgtctgt tcctaattca
     9781 gtttgatgaa ttaactgata aaaaaatatc tactatttaa agatacaggg ataagtaagt
     9841 aaaaaaataa catgtcatta gatagtagac agttccaact ctttatttac cttttttgta
     9901 tgctgcactg tcaatccaag ttcctaaatt gttgtatcca tcatcactta tataatcata
     9961 ttggccatca ataatttcgt caaaccacaa atattctcca tctggttttt gcccaattat
    10021 tttaattact gattgactag tagttgtcat ctggttaaaa tcttttattt tacctatatt
    10081 aacggccaaa atagcatttc ctcgcttata actctcctgg atttcatatt taacatattc
    10141 tcgatcaaga gtttctttac ctatcagcac cacagtcact gatgttccaa ttaattgatt
    10201 atctatccag ttttttatag caatatctcc attacgttta actttctcaa attcagcctt
    10261 atctacaaaa ccagcttcct ttgttcctcg caaagtccag ctgtttctaa ctaccattgc
    10321 tcttgttaca tctgcataat ggaaactaaa aaaaaccctt ctcaccatat taatcctcct
    10381 gaatactatt tattaaactg acaatttgtt caactttttt atctacttct ccttcagtat
    10441 ctaatttttc tagcgcttga aatttacttt ttaaggaatc atcaaaataa tcactgaaat
    10501 tctcttgaat cttatcaaac aaaaccttac ttgcaccttt ggtaaatcca atcggaacta
    10561 ctatattttc attttgaaca gataattcaa attcttcaat cattccatct gctgtaacta
    10621 tttcacctga ttcaagtttg tttccaaaaa caaaaatact aatcccagac tctgcaataa
    10681 tgttttctcg atatcttctc cactcatgag gcatatcaag gggaagtggt cttaaagaaa
    10741 gtacgtcttg tatgttattt tttcttaaat ttcttctctg ctttagaact cctgaaacaa
    10801 tactattacc aattccaagt ccgtaacctg atactattct ataaccggat tcagaaagcc
    10861 tatatgctaa tttctcgaca aattttaatc ctttcgattc agatgtaaat ggagtataat
    10921 caacagcact tcccgaaata aaaatagttt ttctcaaata cttttttcta atacttttca
    10981 aaatatcagt aatctcagaa taatcatcta cttcaacaac gtttatcgaa aatctggaca
    11041 aatcattgat ttgtagtctt ctttttgtca gttcataatt atatctctct tgatcaaaaa
    11101 aatcctcaga atcgacaaaa ttttccttta aaattatatg ggattttaga ttatcttttt
    11161 agaagttctt gaatgtcggt atagcccgtg tttccgggct tttccggcat ttttgatatt
    11221 gttagatact tacaaatgtt cttattacct ctcaccattt ccccctcaaa agtagtaaat
    11281 ggagtagtaa aaccggcctt gtgtcaggct gccagccgtt ccatttctgc ccttgcggaa
    11341 tcgaatgtgg cgtgtgcata atagttcagc gtcatggtga tattggaatg tcccataatg
    11401 tactgcaagg ctttggggtt cattcccgca ttggctaacc gggtgcagaa tgtatgacgc
    11461 atagcatggg gcgtcgtcgt tttcggcaac gcttcttcat ggctcttgtt gtacttcttc
    11521 acaagcctgc caaacatccc gccataatct accgccgtca tgggatagcc gttctgtttg
    11581 agaaacagga aattggcata cccgtcaata atgaacggct ttgcaccttt gcggtttttt
    11641 atcactcgct ggaacgcctg atacacttcc tcattcatgg ggatttttct gacgccgctt
    11701 tgggtcttgg gttcgtcaat atagtaaccg acgccggtat ttctcaaaag ctggtggctc
    11761 acgatgatta cccggttttc aaaatccaaa tctttgtccg tcagtccgca cagttccgaa
    11821 atgcgaagcc ctgtccccag cagaataacg attgcgtcat aatgcttttg gtaaacctta
    11881 tcaccttgaa taaaggacag aaaactttct tcctgtgctg gtgtaagagg cacctttggt
    11941 tccgtatcgt cgtccagaac gtcactaagc tggaagtcaa acgggttttt tcggatgcaa
    12001 tcgtcctgta tggcggtgta aaaagccgct ttcagggaac gcttgtcgtt attgatggtc
    12061 ttataagaaa ggcccttttc tttcatccga agcgcccatt cctttgcgtc ggacagcttc
    12121 acactgtcaa tcgggcagct ccccagccga tcttgttcca gaatacgcat cagccgttcc
    12181 cggcccagtt tggtgttgtg cttcacattg ccccggtgcc tgatctgctt ggcgtagagc
    12241 tggcagacgg tcattttctt gccgatggtg tctatcccgt cgtcaaggtc tttctggatt
    12301 tctttctcct tttcccggag tgaaatatcc tcccgtttac ctgccggggt cttatccgta
    12361 ggcactaact tccatgagta aacaaactgc ggttttccaa aggtatcaac atatttgtaa
    12421 gcatatcgtc cgtcttttct ctggctctct ccagaccgca aaacgcgatt tttgctgtct
    12481 cgtctttttt ctgacatctc gtcatgctcc tttccatgac ggaaagagcc gcatatatgc
    12541 ttataaatta ttataacata tctgcggccc tttttcacta ttagattgtg tccaaagtgt
    12601 caattatttt ctcaaactgc ttgcgtttga tctggatgcg gttgccattc ataatgaccc
    12661 agccagaggc cggattttcc tcggccagct tacgcaattt gttttctccg atacggaaat
    12721 atttagacgc ttcctcaatc gtcagcgtgt atttttccca aataggcaca tcatggttca
    12781 taatgcagcc ccctttcaca aattcaaagg tttgtgtgat ttaggggcat agacggacgg
    12841 ctgctggcac agctccacgg gactttcacc cccgcgtggt tctcacgcgg ccctacccat
    12901 tgcctgcgac gcttttaacg ctcggactgt ggctataagg gagtatcata gtcctgcctg
    12961 tacgtcgtcg cccgcaagcc gccacgctca cattgcggcg tggtgttcct cgctccgctt
    13021 ttcccgtagg gaaaagtatc atggcgcacc agccgtccgg ctcggcacag acagaatgtg
    13081 tccgtatgcc ctatgatgcg ccgccgttat cttgcgggca tatttatcaa ggtgcgatca
    13141 ggccaccggg aaaacaccga aacggggaaa gggtaagccg tttcatgctg ccggggccgg
    13201 ttcgtcagtt cgtgacgata gcgtggatga gcttgatttc cagacggcgc ttcatggcct
    13261 cgtccagaca cacatgcgga tagccgtttt cgtcatagag cgtccgggta cacagcttgt
    13321 tcatgtaacc gctgtaatac tgcaaaacgc gctctacggc tccggcatcc ccagcgcggg
    13381 cggcctcgat caccgaaagg ggcaaaagct caatgccctt gtagttcggg ttcttcatcc
    13441 gcgtctaacc tcccttcggc atcaacgcct ttagctgttt ccgcagctcc gataaggtat
    13501 ttgtcctgtg ccgctgaacg gcactccggg acattcccac aaggccgccg atctcaccat
    13561 ctgccatatc cagaacacag tgcaggatta aaatactctg ctccatgctg ggcagcgcgg
    13621 caaaggcttc ggcaacaagc tcattgttga tatgtaggtc atagccatgc gacgaaaacg
    13681 taaagctgtc gctggggtag tgatctactg tacagagctt gtccaaatct gcctgtgaca
    13741 aagagcttag tgacacttca cggtcacggc ggcgcttcat ttcagccaag tagcttttgg
    13801 cctcgttacg cagtaccgtc ttgcaaaaag catcgaacat gcaccgcgct tcatagtcgc
    13861 gggggatagc atccatcttc tcaccccctt tctgtggggg acttaggtgg ttctctccct
    13921 ttccgctaac aagactttga caaggccgcc agctacccgc taaatacctc ctttttgaaa
    13981 atttctcaaa aaattttttt ggggacagcc gggccgcaaa agacgacaaa aatcgcccgg
    14041 caggtcgcaa acctaccggg cgagaaaatg ctttgctatg ctgctgattt acatggtata
    14101 gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DAYYHS010000071        14129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000071 DAYYHS010000000
VERSION     DAYYHS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.071"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHA6A_002159"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHA6A_002159"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8970328.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHA6A_002160"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHA6A_002160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8970329.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHA6A_002161"
     CDS             8118..9086
                     /locus_tag="ACHA6A_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8970330.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHA6A_002162"
     CDS             9104..10009
                     /locus_tag="ACHA6A_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8970331.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHA6A_002163"
     CDS             10006..10812
                     /locus_tag="ACHA6A_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970332.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHA6A_002164"
     CDS             10969..11937
                     /locus_tag="ACHA6A_002164"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8970333.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHA6A_002165"
     CDS             complement(12254..12778)
                     /locus_tag="ACHA6A_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8970334.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="ACHA6A_002166"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="ACHA6A_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8970335.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3996 a         3046 c         2791 g         4296 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAYYHS010000072        13917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000072 DAYYHS010000000
VERSION     DAYYHS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.072"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA6A_002167"
     CDS             131..505
                     /locus_tag="ACHA6A_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8970336.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA6A_002168"
     CDS             complement(619..1248)
                     /locus_tag="ACHA6A_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8970337.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA6A_002169"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA6A_002169"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACHA6A_002170"
     tRNA            1668..1741
                     /locus_tag="ACHA6A_002170"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACHA6A_002171"
     CDS             complement(1879..2043)
                     /locus_tag="ACHA6A_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970338.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACHA6A_002172"
     CDS             2338..3666
                     /locus_tag="ACHA6A_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8970339.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACHA6A_002173"
     CDS             3868..4701
                     /locus_tag="ACHA6A_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8970340.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACHA6A_002174"
     CDS             4717..5454
                     /locus_tag="ACHA6A_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8970341.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACHA6A_002175"
     CDS             complement(5609..6577)
                     /locus_tag="ACHA6A_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8970342.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACHA6A_002176"
     CDS             6809..7642
                     /locus_tag="ACHA6A_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8970343.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACHA6A_002177"
     CDS             7676..8515
                     /locus_tag="ACHA6A_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8970344.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACHA6A_002178"
     CDS             8541..9101
                     /locus_tag="ACHA6A_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8970345.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACHA6A_002179"
     CDS             9300..11021
                     /locus_tag="ACHA6A_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8970346.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACHA6A_002180"
     CDS             11186..12328
                     /locus_tag="ACHA6A_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8970347.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACHA6A_002181"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACHA6A_002181"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8970348.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLFNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2832 g         4036 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tatttaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAYYHS010000073        13903 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000073 DAYYHS010000000
VERSION     DAYYHS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.073"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..2222)
                     /gene="glyS"
                     /locus_tag="ACHA6A_002182"
     CDS             complement(147..2222)
                     /gene="glyS"
                     /locus_tag="ACHA6A_002182"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8970349.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2224..3141)
                     /gene="glyQ"
                     /locus_tag="ACHA6A_002183"
     CDS             complement(2224..3141)
                     /gene="glyQ"
                     /locus_tag="ACHA6A_002183"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8970350.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3525..4337)
                     /gene="recO"
                     /locus_tag="ACHA6A_002184"
     CDS             complement(3525..4337)
                     /gene="recO"
                     /locus_tag="ACHA6A_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8970351.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4480..5379)
                     /gene="era"
                     /locus_tag="ACHA6A_002185"
     CDS             complement(4480..5379)
                     /gene="era"
                     /locus_tag="ACHA6A_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8970352.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5394..5789)
                     /locus_tag="ACHA6A_002186"
     CDS             complement(5394..5789)
                     /locus_tag="ACHA6A_002186"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8970353.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5773..6249)
                     /gene="ybeY"
                     /locus_tag="ACHA6A_002187"
     CDS             complement(5773..6249)
                     /gene="ybeY"
                     /locus_tag="ACHA6A_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8970354.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6266..8458)
                     /locus_tag="ACHA6A_002188"
     CDS             complement(6266..8458)
                     /locus_tag="ACHA6A_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8970355.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8482..9453)
                     /locus_tag="ACHA6A_002189"
     CDS             complement(8482..9453)
                     /locus_tag="ACHA6A_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8970356.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10159..11538
                     /gene="brnQ"
                     /locus_tag="ACHA6A_002190"
     CDS             10159..11538
                     /gene="brnQ"
                     /locus_tag="ACHA6A_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8970357.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11692..12138)
                     /locus_tag="ACHA6A_002191"
     CDS             complement(11692..12138)
                     /locus_tag="ACHA6A_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8970358.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12165..12341)
                     /gene="rpsU"
                     /locus_tag="ACHA6A_002192"
     CDS             complement(12165..12341)
                     /gene="rpsU"
                     /locus_tag="ACHA6A_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8970359.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12503..12931)
                     /locus_tag="ACHA6A_002193"
     CDS             complement(12503..12931)
                     /locus_tag="ACHA6A_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8970360.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13023..13883
                     /locus_tag="ACHA6A_002194"
     CDS             13023..13883
                     /locus_tag="ACHA6A_002194"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8970361.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3823 a         2882 c         2501 g         4697 t
ORIGIN      
        1 tcataggtgt ttttaaaagt tacacaaaaa ataaaataac aaaaaaatta acattgaatt
       61 aataatagtt atttatattt ataacaaata aaccagagaa atagcctatt ttgtgcctgt
      121 tctctggttt atttacatat tagaaattat tttgtgatga tttgatctaa acttgctagt
      181 gacaaagcca tggcagcaat ttgacttaat tgcgttaatc ggttcttacg tactgctggt
      241 tcttctgcca tgaccatcgt ctgatcaaaa taattttcga tcagtgggcg aaggcttaca
      301 agctgatcat aattttcgtt gatcgtttgg tcagcaaagc ttgttttcat tgcctctact
      361 gctttgtgca gttctttctc cgcttcgttt tcgaacagtt cagggtctac ttctgattgt
      421 ttttcaagct ccgctttttt cgctaagttc acgacacgtg tcaaagcttc catcgatggt
      481 ttgaagtctt catctgcaag atgctgcttg aacaattgtg ctgctgcaaa tacttttgtc
      541 agatcttttt gttctgcatt caaaacagct tcgatgacat catgacggat attcttcgtt
      601 gtcaataatt ggcgcagacg gcctttgata aagtcgatca cctgttgttg tcccgctgac
      661 aagccaatgc caaaacgatc cgtatcttta ctgataactt cactaatcgt ttcttgcaga
      721 acagacaaag gaaaggccca ttttttcgat tcgatgattc gaacgacacc atatgcttga
      781 cggcgcaaag catatggatc gtttgagcca cttgggatca tcccaactgc aaagaaagaa
      841 aagatactat ctaatttatc agcaatcgct aaaacagaac ctactgtagt ctcaggtaaa
      901 tctccttcac tggatatagg catgtaatgt tcacggatcg ctgttgctac ggctggtttc
      961 tcgcctttaa gcagagcata tttttcgccc atgatccctt gtagttcagg gaattctcct
     1021 accatgttgg ttactagatc aaatttatag atttcagcag cgcgttgcag atccatcaat
     1081 tcttcctcag atagtcctac ttgttcgcct atgatgcgag cgatgacttg tacccgttgc
     1141 attttctcat agatgctacc gattttttca tggaaagtaa cagtttttaa tttttcaacg
     1201 tattggtcga ttgaagcttt cttgtcttca ttataaaaga attccgcatc ttctaaacga
     1261 gcagtcaata ctttttgatt gcctcgtacc acattttcca attttacccg atcgccatta
     1321 cgagcagaaa taaaatgagg aagcaacata ccttgatcgt tccgtacttc gaaataacgt
     1381 tgatgttctt tcatagaagt gactagtact tcttcaggaa cagatagata tttttcttcg
     1441 aagtcgccag aaaagacagt tggatattcg actaaattgt tgacttcttc tagtaaatct
     1501 tcatctagat caatgatcca gtgatttttt tctgctaatt cgtttgcttg tgtgatgatc
     1561 atttcttttc ttttttgcgg tgaagcaata acaaattgtt cattcaattt ttcttcgtat
     1621 tgttttgcat gcgtaaatgt cacatcttcg cctaagaaac gatgacctct tgaagctctg
     1681 tctgttttga catccaagat ctcaaacggg attatttcgt catctaacaa tgccacgatc
     1741 cagtgaatag ggcgaatata ttcaaaatcg tattctgccc agtgcatcgt tactgggaaa
     1801 gtgaggcttt taatgacatc tgctaaacct gtcaatactt cttttgcttc tttgccgtga
     1861 gtgtatttcg ttacatagac atattccacg ccttttaatt ctctaaaagt gatagcatct
     1921 gtcgtcagac cttgtccgcg gacaaatcct tcagctgctt tcgtccagtt tccatctgcg
     1981 tccaaagcaa tttttttggc tggtcctttg acttcttctt cgctgtctgc ttgacgttct
     2041 ggaatgtttg ttactttgat tgctaagcgg cgcggtgtag aaaacgtttc gatcgtatca
     2101 taagtcaatt ggttttcatc taaaaatttt gctgtttttt cttctaattg tttcatgctt
     2161 ggtgtgacta catgagcagg tatttcttcc agcccgattt caagtaataa gtcttttgcc
     2221 atcttattca gcctccttca cgttttctgg atttgctgtg tttttattca ataaagggaa
     2281 tcctaatttt tcgcgttctg ccacaaagat tttggctaca gcacgagcca tgttgcggat
     2341 acgggaaaga taacctgcac gctctgttac ggatactgca ccgcgagcat ctaataaatt
     2401 gaatgtatgg ctacatttca aaatataatc atacgctgga tgaacaagat tttcatcgat
     2461 acagcgttta gcttcttttt caaaacgatc aaaattgctt agtagcaggt cttggtcgct
     2521 tacttcaaaa gaatatttag aatgttcata ttctggttgt ttgaagatct caccatattt
     2581 cacgccatca gcccattcca gatcataaac actttcaact tcttggatat aagaagcaag
     2641 acgttctagt ccataagtga tctcagaagt aactggttta caaggtagtc ctccgacttg
     2701 ctggaagtat gtgaattgtg tgatctccat cccatcaagc catacttccc agcccacacc
     2761 ggcacaaccc atcgatgggt tctcccagtt gtcttcaaca aagcgaatat cgtgttctaa
     2821 tggatcaatt cctaaaagac gcaagctttc taaataaagc tcttggatat tttcaggtga
     2881 aggtttcatt acgacttgaa attgatgatg ctgatacaaa cggtttgggt tttctccata
     2941 acgcccgtca gctggtcggc gggatggctc tacataagca gcattccatg gttccggtcc
     3001 gattgcccgt aagaatgtat atgggctcat cgtacctgct cctttttctg tatcataagc
     3061 ttgcatcagc atgcagccgt ttgatgacca aaatttttgt aatgttaaaa tcatatcttg
     3121 gacggtcaat tttttactca tctgttttgc ccctttcaat ttttaagcgt tgctgtcaat
     3181 aagaatacag gaaagaaaac gacaaaaagt ccctatgtct gattacagac atagggacga
     3241 ataatttcgc ggttccaccc tacttccgaa taaagattcg gcagcttcat ttatcgtact
     3301 cgcaagtgcc tcttcaaaaa tcgtttgggt ccggcttcca ctctcccgaa ctcgctaaac
     3361 cacgtgtttt ttactgatct tggtcaatgt acgtattcat tttgcagttt tctggtttgt
     3421 ataatagatc ctctcagtga acgatctatt taatctacgg ttaataatag acggaagtcc
     3481 ctgtatttgt caaatagttt tgattttttt gttgtatctt tcatttattc ttctttttca
     3541 ggtatctctt tttcaggcat atttttctct ggcgtcttta atacgtgtgc ccaagacttc
     3601 atttgatcaa tgaacttttt gctttttaga tgaataccga catattcatc atacaactgg
     3661 tcaatgaggt cacggattgc ctgcttcgtt tcttctttca agtggattcc actgatctta
     3721 tcataagaaa tagccgaaaa cattcgaacg aaataaacag cgcgaggatc tgcatgataa
     3781 cggtgttcgt ccattggcca gtgtttctcg catatcacac cgccatattt ggaagaatag
     3841 tcaaattttc cttttgtttc cccgcatacg acacagtgcg tccaaattgg cgcgattccg
     3901 aagcgttgca gtatttgcac ttcaaagata ttcgtgatga tttctgcatc atctccttgg
     3961 tccatccgct gcagagcttg ttgcgtaaat tggaataaat gcgggtcata ttgatgatct
     4021 tcgattgctg catctactag atttagcaaa tacgtaccat aaccgctcaa aaagatatct
     4081 tcttggatgt tccgaaacgc atggacgtct ttgacactgc ttaaaaacga caaaccttca
     4141 cttcgaaaat taccgatata gactgcttcg gtaaagggga gcaaagccgg agctaatggc
     4201 tgattttgcc gatggattcc tttgatataa aacatcattt tgccagaaga ttccgtaaag
     4261 attttgacta atttatcttt ttctttgtaa ttacggctag ataaaatcaa gcctttcgat
     4321 tctgcaagct ggctcatatt ctccctccta aaaaataaat ctggaaaagc agattctgcc
     4381 gatccagatt atttatgttt cgatcttccg ctgtttttct caacgtacgg atttttatcc
     4441 agtttatttc tatacctaac ctataagaag tccagaatat cagtactcgt cttttcggta
     4501 gccgaagtct tgcagataga cttttttgtc tcgccagtct ttttggactt tgacccaaag
     4561 ttctagaaat actttgtctc ctaaaagatt ttcgatatcc tttcttgctt tcgttcctac
     4621 ttgcttcagc atttttccgc ctttgccgat aatgattcct ttttggctat ctctttcgac
     4681 gatgatcgtg gcctggatat ggattttatc attctcgttt cgcttcatcg aatcaacaac
     4741 gactgcgaca gaatgaggca cttcgtctcg tgttaaaaac aagactttct cacggatcaa
     4801 ttcagaaacg atgaagtatt caggatgatc agtcacttga tcatctggaa aatattgtgg
     4861 gccctctggc atttcactga ctaatacatc cattaagcgt tcgacattgt ttccttctgt
     4921 agcagagatc ggaatcactt gtgcaaaagg catctgcgtc gaataatctt cgatgattcc
     4981 cagcaactcg tctggatgga tcttatcgat tttattgata atcagataaa caggagaatg
     5041 cacgtttttt agacgttcaa taataaaatc gtctccccgg ccacgttttt gatcagcact
     5101 gatcataaat aagactgcat ccacttcttt caacgcactg taagctgctt ctaccataaa
     5161 atctcctaga cgatgctttg gtttatggat tcctggtgta tcgataaaaa tcagctgagc
     5221 ttctggtgta gtatatactc cttggatttt atttctagtt gtctgtgcct tgtcactcat
     5281 aatggcaatt ttttgaccca cgattcggtt caataatgtg gattttccta cattcgggcg
     5341 gccgacgatt gcgacaaacc ctgatttatg ttctggcata tacgttctcc tttttaatta
     5401 aacaacaaat gatatatttt agggacaaat aaaatcaacc caatgatgac agcaaaacaa
     5461 gcggtcacta aaactgctcc agctgccata tctttgattt ttttcccaat cggatgaaaa
     5521 tgaaaatccg taaacatgtc cactacattt tcaaaaacag tattgatgat ctctactatc
     5581 caaacgagaa aaacagctaa aagcagccat aaccactcta tccgatcaag ctggaaaatc
     5641 acgcccataa ataaagcaag gataccgaac gcaacatgtt tcctcatatt gcgttcttcc
     5701 tcaaaaacgg ttttgactcc ttggacagca aattccacag acatcaggaa atgtttgttt
     5761 ttctctacgt ttttatcttt tgagtccata ggcatccaag atctctttct gtaaaccaaa
     5821 catttctttc tcatcttctg gtgtcatatg gtcatacccg ttgatatgca agaatccatg
     5881 taccgctaaa aagccaagtt cccgatcata tgaatggccg taagcttctg cttgttcagc
     5941 agcacgttca atagagatca tgatgtctcc tagtacacga ggtaatggat tctctccatc
     6001 ttcaaaaatc actgggattt cgtcttcgcc ttcttcttcc agagcaaaac tgatgacgtc
     6061 tgtcggtgca tcttttccac ggtaatcacg attgattact tggattgccg cattgtccat
     6121 gaaagtaaca gacatttctg tttcctctgg cagctttaaa taagatgctg caaattgcag
     6181 caagtcatcg atttctttca ttttctcttc tgagacttgg tttgtttcat ccataaaggt
     6241 aatatccatt atacatcgct tctttctatt cttcttgttc ttcctttatc ttctcaactt
     6301 ctgttttcat tttaggatat ttgatccgag aatggaaggt gctacttaaa ccgctgatta
     6361 atgatttttc cacttttcgg atttctttca aggtcagtcc actatcgtct aattgaccat
     6421 cggaaatacg ctcttcgata agattatgga caaaactttc gattttttcg ctcgtcggat
     6481 gatccattgc ccgtactgca gcctcacaac tgtcagcaat attgacgatc cctgcttcac
     6541 gactttgcgg ttttggtcca ggataacgga attgctcttc tgttacttcc ggattgcgtt
     6601 ctttcgcttt gatgtagaaa taacgcatta gcgtggttcc atgatgctgt ttgcaaatat
     6661 cgatcaccat ttgcggcata ttataatctt ccaatatttt ggctccatct gtcacatgtc
     6721 caaagataat atgtttactg tcttctggca ataagaaatt gtgcgggttt tccgctcctg
     6781 aaggcaagtt ttcaacgaaa aagctagcat gtttgatctt gccgatatca tgataatagc
     6841 aagctactcg agttagcaaa gaacgtccgc cgatttctgc tacagcgtta gcacttagat
     6901 tagctaccat catactatga tgataagtac ctggcgcttc ttccagcaac tgtttcaata
     6961 aaggatgatt tggattgctg agttcattta atacaattac actgtcatca ttgaccatca
     7021 attcaatata tgggtgtaga ccgatcgtca gcaagaatga gaagagtgca ccagtgaacc
     7081 cgcagacgag cattaaccaa gtcgttgcat caccaaagct cattccttga tagacgatca
     7141 aaatgacatc catcatcaca ggaaaaataa tgacccacat ggcagcagat aatccttgtt
     7201 ctgagatccg tttgcgtctg acaatcgttg ccatgagccc agagaaaaca taagacatca
     7261 agataatcgt tagaaagttc gtgccaatcg aatcataaaa aatgaaaagt gcggatactg
     7321 cttggaaaat accagccatg atccctgctc ttcgattcaa gaaaaagctt aaaacaagtg
     7381 gtgcaaaagc agcgggataa aacagcggga tataagtagc ttgttccgtt tggaataatt
     7441 ggaaaaattt catcagcact atgctgactg acatagctgt cacatagaaa agcacgtatt
     7501 ttgttctttc tcctgcattt tggaattgaa gtgaattata aatcagtacg ccgatttgaa
     7561 gcaaaacagc taaaaccatg gcaattagcg gaaaaatcga agttgtttga ttagtcagtc
     7621 ctagtaattc caattttttc atagctgaag catcgatttg attaccttca cgaacaatga
     7681 tttctccttg gaagatcatc actggttgta cagcttctcg tgcggattgt ttcaattctt
     7741 ctgtcttttt ctcattcaat acgtcattca ctacgatacc ttgatccact aagtagcgaa
     7801 tcatttgttg ttgtgctggt gtgacattca aatattggat ttgctcttct gcttcttgtt
     7861 taaaagcttc caattcactt tcacgaactt gtttgctcat ctgttcgtca atgagcttca
     7921 gactctcatc cctcacagta tgaatctcag tttcagtcat ttcaaatatc gtcgaataaa
     7981 aattgttcgg tagtttttga taaaacacaa cattttctgc attcacattt tcaaaatttt
     8041 tcttcagcgc ggcgatccgc tcttctgtag taggctttgg gatattttca tcttcctttg
     8101 ccttatcaat cttttcgtca taagatttgt tgatggcatc attcgtctta tcgatcaatt
     8161 caaatagttg cttgatccga ttgttctggt catctgcaag atctttttgg taagtgtatt
     8221 ctggagtgac tgcttctgct gccagttttc ttttttgttc ggtttcttcc gtgttctcga
     8281 tcgttttgtt cgcacggata ctttcttctg ctacttggcc ttctctgaaa tcattggcct
     8341 tttgtttgac actactaaat gtaacgccgc agataatgat aaaaaacaag aacagcagga
     8401 aaggcaatgc tgcctttcca agtttttctt gtagtttctg cagtgtttta ttcaacatgt
     8461 gcttatcctc ctaagcaaag cttattcttg atggagatct gccttttcat aagctttgat
     8521 gatttcggca actacagggt gccgaacaac gtcacttgct tcaaaattaa caaaatcgat
     8581 tttcttgatt tgctttaatg ttcgttctgc atgaacaaga ccacttgtcg tccctcgagg
     8641 caagtctatc tgacttgtat ccccattgac aatcattttt gattgataac cgagacgggt
     8701 caagaacatt ttcatctgag cgactgtcgt gttttgtgct tcgtccaaga taacgaatgc
     8761 atcatccaat gttcgccccc gcatgtaagc taaaggtgcg atttcgatta cgcctcgttc
     8821 catcaatcgg ttcgtatgat ccaatccaaa aatttggtat aaagcatcat aaacaggccg
     8881 cagataagga tccacttttt cttttagatc cccgggaaga aagcctagat tttcacctgc
     8941 ttccacagca ggccgcgtca aaatgatttt ttgcacttca ccttttttca aggcagcgat
     9001 tgctaatact actgcaagaa aagtttttcc tgtaccggct ggtcctacgc caaatacgat
     9061 atcatgtttg gctaccgatt cgacatattt cttttggccg ctgtttttta cccgtatagg
     9121 ttttccatta cggtctttga cgatctcttc ctcatacatc tcaacaaaat aatctaatgt
     9181 acctttttgc cccattttca atgccgtcac aacatcgggc gtacttacgt ggattcctcg
     9241 tttgatcaat tcctgtaatg tccgaatgac tgaggcagcc aaggaaatct gggattgctc
     9301 accaatgatc tgaatgattt ctccacgtgt atggatggtt gtttccgttg tttcctcaat
     9361 cagttttata tgcttgtcgt gtgcacctaa aagcatgctg acatcatcag cagccgtcaa
     9421 tctaatctct aaagaactag actcttccgt caaaaataga catccccttt ttttcgattc
     9481 attctttcct attttaccat actcagaaga aactatatag caaataagaa aaggttctaa
     9541 aagagttgct taaccataag taccaagaaa cgttaaaaaa ctagccgctt ccattctcta
     9601 aaggtctcgt ttgaataaaa aaagctgatc atgtaagcta tgcataagat agaggacaag
     9661 tattccgtct aacttagcaa gtattctctg ggtatttttc ttgtttcaaa aaaagaaaac
     9721 aaaatatttg aaatattctt gcatttcaaa aaaaacaggt gtattataac gtcaacaaat
     9781 aaatatacag caaagcgtcg aggaaaacag taggctattc agtgatttat tcccagtgag
     9841 tcatgtaagt gagaaatgac aataaatcga atagacgaac atcatttccg agtcaatgtc
     9901 gaaaacgtag atcattgtgg ctgcctccat tatcaggcta gcggataact ttatcgaaaa
     9961 gcgtccgtga atgaagaagc ttttttaagc gaaaaaaggt ggtaccgttc tagaatctag
    10021 agcccttttc ccaagtggaa aagggctttt tttatatttt aaggtggtgg ttttcatgga
    10081 atctgatatt agtggtattt ctagtttctg caacgtttat tttgttactc aaaatgataa
    10141 ttattggagg aaaaatttat ggaagaaaca aaaacttatt catggaagca attattgatg
    10201 gtcagttcac ttatcttcgg gatgttcttc gggtcaggta atctgatctt tcctgtacac
    10261 ttaggtcaaa tggctggatc aaactggatc agcgctggtg taggatttgc catctcaggc
    10321 gctcttttcc cattattagc tatcttagct gtagtagtga caaaaagtga tggtctgtat
    10381 gacttagcta aacctgtcgg aaaatggtat gctgccttgt tcttgatcct tgttcattta
    10441 acgatcggac cattctttgg tacaccgcgg acagcagcaa ctgcttttga aatggctgct
    10501 aaaccctttt tgcctgaaaa atatgattca ttaggtatgt ttttattctc tgctcttttc
    10561 tttttacttg cttatttcct tactgtacat ccaacacgtt tggtcaaata tgtcggaaag
    10621 tatttaaact ccttgtttct tgcattatta gctatcattt ttgtcgtttc tttattccaa
    10681 cccatgggga atctaaacca atctgctgca gattcttatc agacgaacgc tttgacaaat
    10741 ggcgttttag aaggatacaa cacggttgat gcagtagctt tacttgcttt aagtgtcact
    10801 tttgtccatg cagttcgtga tcttggtttt aaagataaaa aagtcacaga attaacagca
    10861 aaagctggta gcctgagcgt tgcgttagaa gtcatgattt attttggtct ggtccttctc
    10921 ggtgcgatga gtttgaataa gatgtcttta tcagaaaatg gcggaactgc actttctcag
    10981 atcactaatt tttatttagg taacttcggt gctagcttct taggggtaat ggtcacatta
    11041 ggtgtcttca ctactgctat gggactcgtt gtttcttttg cacaagattt ccataaactg
    11101 ttccctaaag tcagttatat gacatggctg cggttaacga cttttgtctc ttttgtcgtt
    11161 gcgaatgccg gattggataa tatcatccaa tggtctttac cagtattgat gcttttgtat
    11221 ccattgtcac ttgcgttaat cttactttcc ttaacagcaa aattcttcca aaagagtcca
    11281 tttgtctatc aagtaacgat gttcttttct gccgttccag ccgtattgga tatgttggca
    11341 aattcgcctg cattagtgag tcagcaaact gtcgtagcaa gcttactagg attttatcat
    11401 cattatgttc cttttgctgc attaggtctt ggctgggtcg tcccaacgtt gcttggttac
    11461 gctggcagct tgctctttta ttatgtatat cgcgtatcag gatataaaca agaagcaagt
    11521 gagttaccag aagaataacc aaatcaaaca caatttaaca taagagatat taaacaggaa
    11581 aaaaacagct agaagaaaga ataagattct ttcttctagc tgttttttag ttcttttttt
    11641 acgaacacaa aaaactgcgg ataatcactg tccgcagttt aaatgataaa attaagattg
    11701 taatagttct ttcacaatcg cattcacttg gtttccatct gcttttcctt tgactttagg
    11761 cataattgca cccataactt tgccaaattc tttcattgat gaagcaccag tttgagtgat
    11821 tgcttcttga acaagttgtc gaatttcttc gtcggaaagt tgttccggca tatatttttc
    11881 gactattaca atttcacttt ttactttttc agctaaatcg tcacgtcctg cttcttcaaa
    11941 ctcatgcaaa gaatcacgtc gctgtttcat ttcacgagac aacaccgtta gttcttcttc
    12001 ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt atagatgact tgatcattcg
    12061 gatgacttgt aaagattcct tatctttcgc tttcatcgct gctttcatgt catcgtttaa
    12121 tctacttagt agtgacacac taccaactcc aattcatcac agaactagaa ttttttgcgt
    12181 tttctagctg cttcagattt tttcttgcgt cttacacttg gtttttcgta gaattcacgt
    12241 ttacgagatt cttgtaaagt acccgctttt gacacggaac gtttgaagcg acgaagagca
    12301 tcgtcaagtg attcgttttt gcgaacgact gtttttgaca tgttaattcc ctccctccga
    12361 gcttaaaaag taaccgtaat tgttattgaa atatatctca aaaaacaaga ctgtcctgta
    12421 aatattatat ctaatgctgt tttgaccgtc aaggaaatct tgcttttttt taaaattatc
    12481 aaatgttatt ttatttaaat ccttattgac aattttcaca tcttccgaaa atctcgaatc
    12541 tatgcccttc gatcgtgcat ccacctagct gttcttcgaa ataattcatc ggacacatat
    12601 ggatttcttt cgttgttccg cacactgtac agataaaatg atggtgatgg ccgattttcc
    12661 cctgacagca atgaaaacga aacttcattt ctccatttaa atcggtttct tccagtaaat
    12721 cgatctctgc aaaatcatgg agattccggt aaatcgtatc ataacttacc cctttgaatt
    12781 cctgattcat atagtcaaaa acttctttag cagatacgta gcggttacgc ttcactaaat
    12841 aagttaacaa tgcttctctt tttttagtat atttaaaacc gttatttttc aatgtttcta
    12901 atgattgttc gatcaatgtc tgttttttca tgacttattc ctccaaatca aaatgattac
    12961 gaagtacgca ttctagagta tggtataata aacataaaaa aaatgcaagt gaggttttat
    13021 ccatgtcttc cgattctaca gaaatcgtta ctttttttgt catctccgac tctgctggag
    13081 aaactgcgac aaaactagcg caagctacta tggctcagta tcctaccgtt gagttcaacc
    13141 tattccgcag aacgttcgtg acagatgaaa aaacattaag gcaggcattg caagatgcat
    13201 tagaagaaaa agcacttgtc ctgcatacat taatcaatca agaactgatc gagataacac
    13261 gttctttttg tgaagaacat caattattta gttttgatgt gatgacgcca ccgatatcag
    13321 aaattgaacg tttaactggt atcaagccaa ttcggcaacc tggtgccctc catttattga
    13381 atgagaacta tttcaaacgg atcaaagcaa tggaatttgc tgtaaaatac gatgatggga
    13441 aagatcctcg tggtttttta gaagcagatg tcgttttgct aggcgtatcc cgaacttcca
    13501 aaacgccttt gagcttgttt ttagctaaca aaaatttaaa agttgccaat ttacctttga
    13561 tcccacaagc acatattccc aaacagcttt gggagatcga tcctaaaaaa atcgtgggac
    13621 taacgaataa cccagatatt ttaaataata tccgtaaaga acggatgcgc tcttacggat
    13681 taaaccctga tactgcttat tccgatatcg aaaaaattag agctgaactt gattttgcga
    13741 atgatcttta tgaaaaacta ggatgtgtag tgatcaatgt ggcatcttta tctattgaag
    13801 aaacggcttc gcttattttg aatgctttag acctagaaga tcatagttat tatggaactg
    13861 aaacaacaga aaaaagcgat taatcatttt tgtctttttc tta
//
LOCUS       DAYYHS010000074        13625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000074 DAYYHS010000000
VERSION     DAYYHS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.074"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..273
                     /locus_tag="ACHA6A_002195"
     CDS             <1..273
                     /locus_tag="ACHA6A_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8970362.1"
                     /translation="TDWNNRLQTEQNKFNDAQNTVNSLNQQINQLSNEKNTIKNQLQS
                     TEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTANRSQEIVTEHTGK"
     gene            complement(555..627)
                     /locus_tag="ACHA6A_002196"
     tRNA            complement(555..627)
                     /locus_tag="ACHA6A_002196"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(592..594),aa:Lys,seq:ctt)
     gene            1008..1577
                     /locus_tag="ACHA6A_002197"
     CDS             1008..1577
                     /locus_tag="ACHA6A_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8970363.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1564..2424
                     /locus_tag="ACHA6A_002198"
     CDS             1564..2424
                     /locus_tag="ACHA6A_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8970364.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2614..3318
                     /gene="yycF"
                     /locus_tag="ACHA6A_002199"
     CDS             2614..3318
                     /gene="yycF"
                     /locus_tag="ACHA6A_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8970365.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3323..5158
                     /gene="walK"
                     /locus_tag="ACHA6A_002200"
     CDS             3323..5158
                     /gene="walK"
                     /locus_tag="ACHA6A_002200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8970366.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5155..6471
                     /gene="yycH"
                     /locus_tag="ACHA6A_002201"
     CDS             5155..6471
                     /gene="yycH"
                     /locus_tag="ACHA6A_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8970367.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6472..7341
                     /locus_tag="ACHA6A_002202"
     CDS             6472..7341
                     /locus_tag="ACHA6A_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8970368.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7396..8205
                     /locus_tag="ACHA6A_002203"
     CDS             7396..8205
                     /locus_tag="ACHA6A_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8970369.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8308..8937)
                     /locus_tag="ACHA6A_002204"
     CDS             complement(8308..8937)
                     /locus_tag="ACHA6A_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8970370.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9107..10399
                     /locus_tag="ACHA6A_002205"
     CDS             9107..10399
                     /locus_tag="ACHA6A_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8970371.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTVVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10475..11137)
                     /locus_tag="ACHA6A_002206"
     CDS             complement(10475..11137)
                     /locus_tag="ACHA6A_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8970372.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11362..11646
                     /gene="groES"
                     /locus_tag="ACHA6A_002207"
     CDS             11362..11646
                     /gene="groES"
                     /locus_tag="ACHA6A_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8970373.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11697..13322
                     /gene="groL"
                     /locus_tag="ACHA6A_002208"
     CDS             11697..13322
                     /gene="groL"
                     /locus_tag="ACHA6A_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8970374.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4585 a         2330 c         2899 g         3811 t
ORIGIN      
        1 actgattgga ataatcgttt acaaacagaa caaaataaat ttaatgatgc tcaaaatact
       61 gttaatagtt taaatcaaca aattaatcaa ttgtcaaatg aaaaaaatac tattaaaaac
      121 caattgcaaa gtactgaaca aaaactagaa aaagcgcaat cagaaaacag taatttgaat
      181 aattatattg aaaaactgga aaaagccaaa agcgatgtag aagacacagc aaacagatca
      241 caagaaatcg tgactgaaca tacaggaaaa tagatgcatt ttttagttta aatttcttta
      301 acaaaatagt attttgttgc taaataattt ttatttgaat attctacaat gatatctccc
      361 ctcaaaatgg aggggaagta catactgtga taatctacag gtttattgac tgattaggat
      421 gttattaaaa aataaaatac taaaatgcat acccgaattt catacccgaa aaataaaaaa
      481 tgccaccacg tacactcttt tcgaaactac ctaagtagcc aagaatgtac tggtgacgtg
      541 ggtttggcac ccaatgggcc gtgaggggct cgaacccgcg acccgctgat taagagtcag
      601 ctgctctacc aactgagcta acggcccgta caaaaagtac ttaataatag tagcactaag
      661 aattttaaaa tgcaagacta catttaaaga aaatcagaaa aatctttact tccttatagg
      721 aaatcattca tagattattc acatttgctc gctattatat aagcatacca acaaacaaag
      781 ttcttgcaag agaacaaacc ctaacacttt tttcattttt tactcctttc ccgccgtctc
      841 cccggcgggt ctcctgttct taacgataaa agacaagaag gcttagcctt cttgtttttt
      901 gttttcatgg aatcccgcta atcaaatcac ctagttaaag caactagatt gaatgtttat
      961 cataagtctg atatcatgtt tatgaaatga aaaaaggagc ggttatgatg gaaaaaatgc
     1021 gtcagcaatt aatggagtgg gggaaggaac tagaaacatt ccgtctgcct cactgggaag
     1081 aactccctga cttagaactt tatatggacc aagtcagaac gttagttgat cgttatcttt
     1141 cacctgtgat acaaggagaa aagcatccat tgcttacctc ttctatggta aacaattacg
     1201 ttaaactagg tttgatacca gcaccagtta aaaaaagata taacaaagaa cacgtagcat
     1261 ttttacttgc aatcactacg ttgaaacaag tattgactat tcctgaaatc aaagaaggga
     1321 tattgttcca aggaaaaaca gtaggaattc gtgaagccta taatctattt tgcgatgaac
     1381 aagaagcagc tgtgtggatg gtcagccaat tagcacaagg taaatctcat ccgcaaaaat
     1441 tcgaccaggc aacccctgta gaatatattg ctgtaagagc ggcaacttta tcgtttgcaa
     1501 tgaaacttct tgcggaaaaa acgattgttc tagaaaccga atatttaaaa gaggagaaaa
     1561 caaatgaaaa acaataaaat cgcaatttta gtagattcag gtacggatgt gccagaagaa
     1621 ttgataaaaa aatacgagat gtatatgatc ccattgaaaa ttatttataa agatcaggta
     1681 tatacagata aaattgacat tacctctgaa gagatctatc aacgtttgcc agaggaaatc
     1741 cctggtacat ctttgccgga tggtgagtcg atcacagaaa tctttgagca aatcaaaaaa
     1801 gacggttatg aaaaggtact tgcggtaaca atttccagtg gattgagcgg gacatataat
     1861 gtggttcgtt taattgcaca acagcaagaa gaattggaag catatgtact agatacgaaa
     1921 aatattggta ttggtgcagg tttttctgcg atccaagcag caaaatggct ggaagaagga
     1981 gtagaatggg atcaactgat cagtaatctg aatgagttag tcgaaagaac aaaagttttc
     2041 tttaatgttg cgacattgga atatcttcaa aaaggtggac ggatcggttt agttgcttct
     2101 attgtaggaa ccgctttaaa gcttaaccca atcatctcat gtaatgaaga agggatttat
     2161 tacacagtag gaaaagctag gggacgtaaa aaaagtttgg ataaaacggt tgcttacgtc
     2221 aaagaaaaaa tcggtaatgc aaaggtcttc aacttagctg ttgcccatgg ggatgcgaaa
     2281 gaagaagcaa ttgaaatgga agcgagactt aaaagagagt tcccacaagc aaaccaaatc
     2341 tactttggcc agatttctcc ggcattagta gtccacactg gtccgggatt attgggtgtc
     2401 ggtgtacaag ttttagaagg ataaatcaaa aaaaggaaac actgaggtga aaatcagtgt
     2461 tttccttttt tattgaagtt agaacaaaaa ataataaagt attttgcact ttcattttaa
     2521 ttttctcttt tgttttatag gcaaagatga aacaaaagca gaaatacggt ataatgagaa
     2581 ggtatgacaa taatatataa gaggtggctc tttatgaaaa aagttttagt tgtcgatgat
     2641 gagaagccga tttcggatat cgtcaaattc aatttagcca aagaaggcta tgatgtttat
     2701 actgcatatg atggggaaga agccttggaa aaagttgctg aggtagaacc tgatttgatt
     2761 ttacttgatt tgatgctacc taaaatggac ggcttagaag ttgctcgtga ggtacgcaaa
     2821 acttatgata tgccgatcat tatggtaaca gccaaagatt ctgaaattga taaagtccta
     2881 ggcttagagt taggcgccga tgattacgtg accaagccat tttccaaccg agaattagtt
     2941 gcacgtgtca aagcaaatct tcgtcgcggt gccacggctg cgaaggaacc agaagaggca
     3001 gcacctgcgg aattgacgat tggtgatttg acgatccatc cagaagctta catggtgacg
     3061 aaacgaggag aaacaatcga gttaacacat cgtgaattcg aactactctt ttacttggcg
     3121 aagcaccttg gacaagtgat gacaagagaa catttattac aaacggtttg gggttatgat
     3181 tattttggag atgtacgaac agtggatgta actgtacgtc gtttgcgtga aaagatcgaa
     3241 gataatccaa gccatccaaa ttatttagtg acacgtcgtg gtgtcggtta ctatttgcgt
     3301 aacccagaac aggagtaatt ggatgaaaag aaaagtccgc ttttttcgat cagtcaattt
     3361 taagatcgct attaccttta ttctgatttt attgatctcg atcgaaatta ttggtgccta
     3421 ctttatacgt ggtttagaac gttccacgat caatacattt attaaagata tgaatcaaac
     3481 ggtggaatca ttagcaacaa caatcagccc ggaattgaat cgaaaagata atgcagatga
     3541 tgaagaggta aatgcgaata tcaaaagatt tattgaaaac agtgcgacta gcgatatcat
     3601 cgaaatacgg gtcgttgacg aaaaagggat catccgagga acaaccgatg tcaatgaaca
     3661 aagcgctgtg ggaaagaaaa atgattatgt tgatatcaat gatttcacga ctaaacgtta
     3721 tgttgcttta gataacgacg ataaacgggt gaatatcaat gttcaaccga tcctgtcccc
     3781 gaccggcgat gccgttatcg gggctcttta cgtcaaaagt aatatcgaac aaaaatattc
     3841 ggaaatcaat aacacagcag tcattttctt tactgcctca ctaattgcgg catttatttc
     3901 aatgatcgta tctgttttag ttgctcgttc gattacacag ccgatcggtg aaatgagaga
     3961 acaagccatt cgaatcgcta gaggagacta cagtcggaaa gtcaaagtgc atggacaaga
     4021 tgaacttgga cagctggctg ataccttcaa tcagttagct gaacgtattg aagaagctca
     4081 agatacaatg gaagctgaac gcaatcgttt ggatagcgtg ctatcccata tgacagacgg
     4141 cgtcattgcg acagatcgca ggggtaaagt cattacgatc aatgatatgg ctgtttcctt
     4201 gctagatatc aaaaacgagg aagcaatcgg acaatcgatt ttgacacttt tagatatcga
     4261 agaagaatat acgttgcgga aattattaga agatccaaac gaaatggtac ttgaccgttc
     4321 aaccagtaca ttggaaagtg atcagatgat cttgcgagta gacttttcta tgatccgtcg
     4381 cgaatcaggt tttatcagcg gattagtagc tgtcttgcat gatgtgacag aacaagaaaa
     4441 aacagaacgg gaacgtcgtg aattcgtctc taatgtttcc catgaacttc gtacaccgtt
     4501 gacaagtatg cgtagttata tcgaagcatt gagcgaaggt gcgtggaaag acgaggaagt
     4561 agcaccaaat tttttgaagg taacgctaga tgaaacagac cgaatgattc gaatgatcaa
     4621 tgacctctta aatctatctc gcatggatac aggtaatacc cagcttcaat tagaatatgt
     4681 caatttcaac gagttagtga attttgttct tgaccggttt gatatgatgg tgggaaatca
     4741 ggaaaaaaat tataagatcc gtcgtgaatt tacgcaaaga gatctttggg tcgaagtaga
     4801 tacagataaa atcatccaag tagtcgataa tatcatgaat aacgcgatca agtattcacc
     4861 agatggggga acgattactt gtcgcttatt agaaacgcat aataacgtgg ttttaagtat
     4921 caccgatcaa ggattgggga ttcctaaaaa agatctgaac cgagtgttcg agcggttcta
     4981 tcgagtagat aaagcacgtg caagagcaca aggtgggaca ggacttggat tagctatttc
     5041 tagagaagtg attaaggcgc atcgaggcgc tatttgggca gagagcaagg aaggaaaagg
     5101 gtcgactttt tatatctcgc tgccatatga accttatgag gaggaatggt gggaatgaga
     5161 atctctgaaa aagtcgttcg tataggactc attttgatgg ttgcgttaag catatacttc
     5221 tcttatgcaa tctggctgag cccagcagga aaaagttcaa tcaacgtaga tgagacaaac
     5281 actcaaatga tcgagtctca aagcaatcga aaaacttccg aagtctttct tccgcttcat
     5341 gttacatggc accattcggg aatgatccaa gaaaccaaca gtgaaaatct cgtgtaccga
     5401 ttgcaattga tcatcgataa agcaagattt ggaaagcttt ctgaagtagt ttcaggagac
     5461 aaatcacagt ttgaacaggt gaaaaatctg aactctggtt ttgagttggc ctataatgcg
     5521 ccttttttgc taagagaata caaggaagca tttgatttag atttagatgt gtcgaacttg
     5581 gcaaaagaaa gcgaaaatat gtattttact agaatacaat tcgatcaagg aaataaaaaa
     5641 atccgatttt tgaattattc gaaccatctc gtatatgaag caaatatttc gttgaattgg
     5701 acagatatca ataaagaatt aacagcaaaa gacacaaaat ggacagaaat gacaacagaa
     5761 ccatcgacga tcgacacgca atatgctacg aaacattcga tcaaactgaa aaagtatagt
     5821 tatatcctgt cacagcaacc ctatacactt tttagaaatg cttttttcca gaaaccagat
     5881 agcgtgaaaa ataatgatga aagcaatgaa ttgatttttt acggtgggaa tgaaacaatg
     5941 tctatccatt cggaaacgca agtagttgat tttcgtggag agctgccaga gaaaaaagaa
     6001 aatgctagtg tattttctga aagtttcccc tatattagca aattaggcag ttctttaggg
     6061 aacctcagat attttgaccg cagtgacaat acgatcgatt atcggatatt cgtggaaggc
     6121 ttcccggttt ttgggacaga cagcagagga cagattggtg tggaagttgg cggtgaaacg
     6181 aatggttctg tgcaagtgaa tatccaaacc agtttgaata cgattcaagt gccgatacct
     6241 tcagacgaag aagtagaact tgcaagtacg aatgaaatca tccagcagtt aaaagctaag
     6301 ggagcagata gcaacaagat tggttcagtg atcgttggct atacatggca aaatgtcaaa
     6361 gaaacgaatc gtgtcgttga tctcttacct gaatggtatg tgaaatataa agataactgg
     6421 tattcagcta acgaccttct ggaaaaatta cctggattgg aggcgaatta attggatttc
     6481 aaaaaaatcg aatggatatt tttcgtcgct tttttagggt tgaatgtgtt tttattcagc
     6541 agttatcatg acgcggtata ccaagaaaac aatgtcattc gctctaatca gactgaatca
     6601 atccaacaac gtctggctag cgacgatatc acttacacgg ggaagatttc gtctgagaac
     6661 aagcaaggct attatcttag cgcggagcag accaatctta gcacttcatt agcgaattat
     6721 cgcaaggctg gtaaaagcgg cttgctggca agtggtgtgt ttttagacgg tgatgttttg
     6781 acgaaatctt tatccagttc agatgctgaa aagaacgtga tcgatccaga taaaattagt
     6841 agttccttgg aagatttttt atctaacaaa gacaatattt tattcggtaa tgattatgtg
     6901 tacacgaaaa acttttctca tttagaagaa gaaccactag aaatccaagc aagccaagag
     6961 tatgaaggca tcccattcaa tgatgataca gcaaaaatcg ttctttcttt agaaaaaaac
     7021 gataatgatt atagcatcac gaaatacatg cagacgcatt tatctgatat cgagcagctg
     7081 cgcgaaaaga cggaactgta tacggaagag gaagcaatca atacattgta catcaataac
     7141 aaaataccaa gaggatcaaa aattttatgg cgtcagctag cttattcctg tattctgaaa
     7201 gtccgggaaa aaaatgtgta tgttcctgtt tggcatgtag cgatcgaaac aagcgataaa
     7261 gtggtccaaa ttgagagtgt caacgctttc agcaatacga tcgtgacaaa caatactgta
     7321 ccaaaagtgg aagatcaata aaaaagcgag tataatgaaa aaagtttaca aagtaagaag
     7381 ggaagtagga gtcaaatggc tgaagaaaaa ccagctttta aaatcagtat cctagcaagt
     7441 ggaagttcgg gtaattcact ttatatcgaa agtgaaaaaa aacggctttt ggtcgatgca
     7501 ggacttagcg gtaaaaaaat cacttccttg atggctcaaa ttgatcggac accggaccaa
     7561 ttagatggaa tacttgtgac gcatgaacat cgagatcata tacatggcgt gggtgtcttg
     7621 gctcgtaagt atcatttgga tgtttttgcc aatcagcaga cttgggaagc gatggaacca
     7681 ctgattggaa atgtaccagt ggaacaaaaa catctatttg agatggggaa agtcagaaca
     7741 ttcggcgata tcgatattga gagtttcggc gtctcccatg acgcagcggc tccgcaattt
     7801 tatcgtttcc ataaagaaaa tcggtcattc gtcatgttga cggacaccgg atattgcagt
     7861 gattatatgc gaggtgtcat tgaaaatgcc gatggttatt tgatggaaag taatcatgat
     7921 cttgagatgt tacgcatggg accttatccg tggaatttaa aacagcgtat tttaggagat
     7981 agaggacatc tttccaacga agacggtgca cttgtcatga ctgatgtgat cggtaatcgg
     8041 accaaacgaa tctatcttgg ccatcttagc aaggaaaaca atatgaaaga acttgctcat
     8101 ttaacaatgg aaaatgtttt gaaagaaaaa gattttggcg ttggacatga ttttgaaatc
     8161 tatgatacag atccagatga agcaactgag ctattttcga tttaaacagt tcaatcaata
     8221 aaaatataaa aagcagaaga taaaatcaat cagggttggt tttatcttct atttttattt
     8281 ttttagcata ttaaaaaaat aaccgtatta tcgactgact acactgatgt gctgcttttc
     8341 atgtttctca tccagtatct catctactaa tgcaatcgcg taatctgcca tgctgatcgt
     8401 actgtcgcca gcagcgtttc tttgtaaata atcatcactt aacaaataag tacctgtacg
     8461 tttgccatgt ggatcaaata aagaagctgg actgatataa gtccaatgta gatttttaga
     8521 ttgtttcaat tttaacaatc cttcgtacat attgaaagct gttggataga agggtgcatt
     8581 tttaggtact tggtcaatca tacgagtcgt cttttctgta tctaagaata acgaagaggc
     8641 acccccaacg acaattaagc gtgtatcggt tccttcaagc atctcaatca aatgggacaa
     8701 agaagtttga tgcatttctt cttttccttt gggagcgttg aaagcatcaa cgacaacatc
     8761 aaaagatgca agatcagtag ttgtcaactc aaataaatcc ttttgtataa acggaacatc
     8821 tgatggcaat ttttctggat gacgcacgat tgccgtcacg tctaaatctc tggacaatgc
     8881 ttcgtctaaa ataaatgaac ctgcatggcc agtagctcca atgagtgcaa ttttcataaa
     8941 aaacctcctt ttttattcct gtccattgta agcagttgct gaatattcgt caaagaatcc
     9001 gatttttgaa aagctcagtg cgcattcctt tcatggttcg tgatcattgg gtatagttaa
     9061 attaagaaaa ctgattgata aaaatagaag ttgaaggaga taagaaatga aagagtggtt
     9121 agatcgtgcc tttttaccaa tgataacatc tgttgtgaat cttagaccct taactgcttt
     9181 gcgtaatgga atgacttaca cgatcccaat aattattgtt ggctcgctgt ttttcttatt
     9241 tgctcatttt cctgtggatt acatcgctca gtggatagac aaaacaggat tttctatgta
     9301 ttttacacaa gtataccagg ctacatttga tgtgatagcc ttttggatcg tttttgccat
     9361 tgcatacgct tatgcaaaag aagaagacat cgatcgctta ccagtcggca taacagcctt
     9421 gtctgcttat ctactgctta ttcagccact aaatactcag atagatactg tctggacagg
     9481 tgctgaaggg atactaggag cagttacaac tgggctcatc atcggatggg gctatgcacg
     9541 tttagtaaga cttgcgaaag ggtggaagca aagagagaag atacctgaaa atgtttggca
     9601 gtcttttact tcattgattc caatcattgt tatagtcact tttacgctta tcggttcagc
     9661 ggtttttcaa cagttgttca gaatgactgt tgttcaagga gtatggacat atgtccagct
     9721 tcccattcaa tcgttcatag acacgttgtt tggtgtgctt ttattaggtt tttttgttcc
     9781 gtttttatgg ttatttggtc ttcatggatc tgcgatggtc aatgggttgg ttagtcccgt
     9841 cttgcaagcg aattcattag cgaatgcaga aatcttagcc tcaggacaag agttgacggt
     9901 ggcaaatggc ggacatatcg ttactcagca atttctcgat cagttcatga cggttacggg
     9961 tgctggattg acgttagggg ctgtattttt tatgacggtt tttgcgaaat cgaagaaata
    10021 cagagaatta ggaaatcttg ctcttttacc agcctttttc aacatcaatg aatcaattat
    10081 tttttccaca ccgatcgtga tgaacccgat gatggctgtt ccttttattt tcgcaccggt
    10141 cgtttcagga ttgattacct attccgcttt atattttgga ttcgttcctt tatttacagt
    10201 agtccaagtc ccttggacaa caccgccgat cctctcgggt ttcttagtag gcggagtgcc
    10261 tgcagcgatt ttacaaggta tcgtgttgtc aattagtttt ttcatttatt tcccattttt
    10321 gaaaaaaata gattctgcaa actgcaaaaa ggaaagacag acaaaaaatg atggagcagc
    10381 agaaaagatg aacgactaaa aaacaaaaat actcctgtcg ctttagattg ggaaaatcac
    10441 caatctagga tgacaggagt atttatagtc atgtttatac tagccctttt tggacagcta
    10501 attgaacaag aacaactaat gtgttcattc ctacgtgtgt gatcatcgat gtccagatcc
    10561 gaccagttgc tttgtactct aggctgaaga agaaacctag gaaaaagtag attagcagat
    10621 gaccatcatt atgagcaaac gcaaacagca aagagctgat cactgctccg acccaaaaat
    10681 ttgaatatcg actgatgatc cccagtatac tgcggcggaa tacgaactct tccatgatgg
    10741 gtccagcaac agttgtcgcc aaaataaaag cgggtgcttc catgatcatc tggatgatat
    10801 tttgtgtatt ctcggaaggg gttgctttgc caaacaatac agattctaac atgacagcga
    10861 tgctttgcag aatcaatgcg acaaagattc caaccaatcc ataaataaca atctttggag
    10921 cagaagcttt ttttaccgct gattcgatcg ctaagggttc acgttgctta aaataaaaaa
    10981 tagctaggac aacggcgcct agtatataca tgactgcagt tgctgtgatc aattcagacg
    11041 tagattttac aagtcctatc gaagctagga tcaagggtga gataaaaatt agaccatagc
    11101 aaaaaatgct gaggaagcta tattttttta aagacatgta attcctcctt ttaagggata
    11161 acaagcatgc aaggcaagct tatatcgatt gatttcaatt atatcataat ttcaataagc
    11221 agctgtcaga ataaaatttc ttttctttga tttaaaagct tgcaaaaaag agggagaatg
    11281 agtattatta aaaatgtagg ttagcactcg aaggttaaga gtgctaacag attaaaaaaa
    11341 gagttatcgg agggatttat cgtgttaaaa ccattaggtg atcgcgtcat tatcgaagtc
    11401 gcaaaagaag aagaaaaaac tgtcggagga atcgttttag cttctgctgc taaagagaaa
    11461 ccccaaactg ggacagttgt tgcagtcggt gaaggccgtt tgttagaaaa cggtgaaaaa
    11521 gttcctgccg ctgtaaaagc aggcgaccaa gttatgttcg aaaaatacgc cggtacagaa
    11581 gtaaaatacg aaggaaaaga atatctgatc gtcgcaggaa aagatatcat ggcgattgtt
    11641 gagtaaattt ggaagataaa aaaataatca aaaataatca atgaggtgaa tcaatcatgg
    11701 caaaagaatt gaaatttgca gaagacgcac gcgcagctat gttacgcggg gtagataaat
    11761 tagcagatac agtgaaagta acattaggac ctaaaggccg aaatgtcgta ctagaaaaat
    11821 cttacggctc tccattgatc acaaatgatg gtgtgacgat cgcaaaagaa atcgaattag
    11881 aagatcattt tgaaaacatg ggtgcaaaac ttgtatcaga agtagcatca aaaacaaatg
    11941 atattgcagg agacggtaca acgacagcaa ctgttctgac acaagcaatc gttcgcgaag
    12001 gtttaaaaaa cgtaactgca ggtgctaatc ctctaggcat tcgtcgcggg atcgaattgg
    12061 caacaaaagc agcagtcgaa gaattgcata atatctcaac tgttgttgat tcaaaagaag
    12121 ccattgcaca agtagcagct gtttcttcgg gttctgataa agttggacat ttgattgctg
    12181 atgcaatgga aaaagttgga aacgacggtg tcatcacaat cgaagaatca aaagggatcg
    12241 aaacagaact agatgttgta gaaggtatgc agtttgatcg tggttactta tcacaatata
    12301 tggtaacaga taacgacaaa atggaagctg ttctagaaaa cccatatatc ttgatcacag
    12361 acaaaaaaat ctctaacatc caagatattc tgccattgtt agaacaaatc ttgcaacaat
    12421 cacgtccatt gttgattatt gctgacgatg tagatggtga agcattgcca actcttgtat
    12481 tgaacaaaat ccgtggaaca ttcaacgtag tagctgtaaa agctcctggt ttcggcgatc
    12541 gtcgtaaagc aatgcttgaa gatatcgcta ttttgacagg tggtacagta attactgacg
    12601 atctaggttt agaattgaaa gatgtaacaa tcgaaaactt aggaaatgct tctaaagttg
    12661 ttgtagataa agacaataca acgatcgtag aaggttctgg agaaaaagaa gcaatcgaag
    12721 ctcgtgtgca attgatcaaa aaccaaattg ctgaaacaac ttctgatttt gaccgcgaaa
    12781 aattacaaga acgcttagct aaattagcag gtggtgtagc tgttgttaaa gttggtgcag
    12841 ctactgaaac agaattgaaa gaattaaaat tacgcattga agatgcattg aatgctacac
    12901 gtgccgcagt agaagaagga atggtctcag gtggtggtac agctctagtg aacgttatca
    12961 gcaaagtttc tgctgtagaa gcagaaggag acgtggcaac aggtatcaag atcgttgttc
    13021 gtgctttaga agaaccaatt cgtcaaatcg ctgaaaacgc tggttatgaa ggatcagtga
    13081 ttgttgataa attgaagaac gtggaactag gcactggttt caatgctgct actggagaat
    13141 gggtaaacat ggttgaagca ggtatcgtag acccaactaa agtaacacgt tctgccttgc
    13201 aaaatgccgc ttccgtttct gccttgttat taacgacaga agcagtcgtt gcagataaac
    13261 cagaaccagc agcgccagca gctcctgcaa tggatccatc aatgatgggc ggtatgatgt
    13321 aagaaaaagc aacttgctta tctacaactg atcattagtg aaagacagat aaaaagaggc
    13381 tggaacagaa gcgtttaact ccaagaaata agaagaaatt cacggaaatt gcttttcaaa
    13441 tttttgtgaa tttcagctta tttccgaagg agttgcttct gcttccgctg tttatacgtg
    13501 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctcttttt tgtgtgtaat
    13561 tataatgaaa atagttattt ttaatagtta taatatttta tctattaaaa gtaaatatcc
    13621 attca
//
LOCUS       DAYYHS010000075        13288 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000075 DAYYHS010000000
VERSION     DAYYHS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.075"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(439..1053)
                     /locus_tag="ACHA6A_002209"
     CDS             complement(439..1053)
                     /locus_tag="ACHA6A_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8970375.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1659..2036)
                     /locus_tag="ACHA6A_002210"
     CDS             complement(1659..2036)
                     /locus_tag="ACHA6A_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970376.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2336..3217)
                     /gene="galU"
                     /locus_tag="ACHA6A_002211"
     CDS             complement(2336..3217)
                     /gene="galU"
                     /locus_tag="ACHA6A_002211"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8970377.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3260..4279)
                     /locus_tag="ACHA6A_002212"
     CDS             complement(3260..4279)
                     /locus_tag="ACHA6A_002212"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8970378.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4912..5916)
                     /locus_tag="ACHA6A_002213"
     CDS             complement(4912..5916)
                     /locus_tag="ACHA6A_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8970379.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5929..6978)
                     /locus_tag="ACHA6A_002214"
     CDS             complement(5929..6978)
                     /locus_tag="ACHA6A_002214"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8970380.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6995..7816)
                     /locus_tag="ACHA6A_002215"
     CDS             complement(6995..7816)
                     /locus_tag="ACHA6A_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8970381.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7803..8582)
                     /locus_tag="ACHA6A_002216"
     CDS             complement(7803..8582)
                     /locus_tag="ACHA6A_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8970382.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8599..9069)
                     /locus_tag="ACHA6A_002217"
     CDS             complement(8599..9069)
                     /locus_tag="ACHA6A_002217"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8970383.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9081..9500)
                     /locus_tag="ACHA6A_002218"
     CDS             complement(9081..9500)
                     /locus_tag="ACHA6A_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8970384.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9581..12358)
                     /locus_tag="ACHA6A_002219"
     CDS             complement(9581..12358)
                     /locus_tag="ACHA6A_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8970385.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12549..12773
                     /locus_tag="ACHA6A_002220"
     CDS             12549..12773
                     /locus_tag="ACHA6A_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970386.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3911 a         2761 c         2157 g         4459 t
ORIGIN      
        1 tttgtgtatt ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg
       61 taaacttcat tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg
      121 gaagactctt tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt
      181 gtctcttttt ccactacttt ttatttttct gacactacat attcttataa ctcatttctc
      241 ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa
      301 gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata
      361 atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact
      421 ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa
      481 atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg
      541 atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa
      601 atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa
      661 atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc
      721 gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac
      781 agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc
      841 tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc
      901 tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt
      961 cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa
     1021 ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga
     1081 catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt
     1141 aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata
     1201 ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa
     1261 ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa
     1321 tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg
     1381 gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta
     1441 tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa
     1501 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
     1561 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
     1621 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
     1681 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
     1741 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
     1801 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
     1861 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
     1921 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
     1981 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
     2041 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
     2101 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
     2161 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
     2221 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
     2281 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
     2341 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
     2401 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
     2461 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     2521 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     2581 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     2641 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     2701 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     2761 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     2821 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     2881 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     2941 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     3001 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     3061 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     3121 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     3181 gcccagcggc aggaatgact gcttttttga ccttcacttg ctgttcctcc taaacgacga
     3241 ttaactattg atcatctatt taacgaagag aaaattcatt ctccattttt ccgtcacgta
     3301 acatgatttc ttttgctgct tttttgatat cttctccatt atacaaaaca ttatagattg
     3361 tttgagtaat tggcatctca acacctagct gttgagctag ctctactgct gcttttgtcg
     3421 ttgccacacc ctcaacgacc attcccatgt tttctagaac ttcttccagc ttatgtccct
     3481 gtcctaacaa gttaccggca cgccaattgc gagaatgaac acttgtacaa gtaacgatca
     3541 agtcccccac accactcaaa ccgataaatg tcagaggatt ggcacccatc gcgacaccaa
     3601 gacggctgat ttctgccaat cctcgagtca tgattgctgc ttttgcatca tctccgaatc
     3661 ctaatccatg gatagcgccg gcaccaattg caatgatatt tttcagtgct gcaccagttt
     3721 ctacgccaat cacatcatta tttgtataaa tcctgaaata atcattcatg aacagttttt
     3781 gtacataggc tgctgctttt tgatcaatac ttgcagctgt aattgttgtg atatcatgta
     3841 ctgccacttc ctcagcatgg ctaggaccag ataatacaac aattgcctgt cttttttctt
     3901 ctggaatttc ttcagccaaa acttctgaga ttcgtttatg cgtatcttgt tctagtcctt
     3961 tacttgcatg tataatgatt ggcttagtat tcattttacc aataagttcc tgtgcgactg
     4021 aacgaatcgc ttttgttgga acaacaaata aaatagcatc tgcgttatcc actgcttctg
     4081 ccaattcttt atatccgata atcgattctg ggatcgtcaa atcaggtaaa aaatgctgat
     4141 ttgtatgata cttattgatt tcatcaatct gttcaggtac atttccccaa atacggacat
     4201 catgcccgtt ttctgccaat gtttgtgcta aagcagtgcc ccaagatcca ggaccaagaa
     4261 ctgcgatttt ttgtttcatg gatattcctc caaataaata attgataatt tccattatag
     4321 tatacatttc ttaaagttgg gactaatttc cattgttttt aagtgtttgt ttacttcttt
     4381 tatcgttttt acttgaaata atcaatgata tgttacaata gtctacattt tatttttaca
     4441 ctcgttatgc tttgcttgtt cctaagagga agcagttttg acaatgttac taccagcatt
     4501 actattagga ggttaggaaa cttctcctag tgagtattga tttaaaatac ataagagaca
     4561 gattgaacat ttctaaatct gtcaggtcct gcaacggaca gattgtctct gttgacggtt
     4621 gacataggaa cttttgtttt agtcaacatt ctggatacaa aacgctatgt aaactggcgc
     4681 aactcgttgt acttggactt ttactacgga tccatgcaac aactaaatag tactattgca
     4741 taacaaaaaa tggttggcac ccacatgtcc cattaaacag attataagtt gaactgtaga
     4801 taatgcaaaa aatgtgtttt ataacgtaat tgatgccaat attttaaaaa aataaaggga
     4861 gcagttatcg ctgctctctt tattttttat attcttttta tccagtctat cctaataatc
     4921 aaactgacga tagtagcggc gtatctctaa tggatggcag ttcaactgct ccacatgagc
     4981 atcgatgcgt tgtgtcacta catgcatcaa taagaaagac aaaacacgtc cgcgatattt
     5041 ttcactgata ccttctactg gatagtcttt tgtgtctatc aatgtataat tgctgcaaat
     5101 ttttggcaaa agtttagcca cacgttcaga taacactctc tgttcatctt cgcctaaaaa
     5161 taatgtgact ggggttgttt cttctacgat ctctaacgtc ccgtgaagaa actcggcagc
     5221 atggatagat ttcgatggaa gccagctttg ttcttcccag taacacatgg catatgaata
     5281 aacagctccc cattgattcc ctgctccaat aaaataatgc atactatcac gtcgatgttt
     5341 ttctgcaaat gataagccaa atgcatctgc ttttttctct gcttccacta accctgttgg
     5401 taaataacgt tcgagctgtt catagtattc tgaataagct tcgaattctc cattcaaata
     5461 catcaatcga tctgctgcca taaaaaattt gatttgttct gttccaggag ctggataagt
     5521 gaccacaaaa tcgcatagct gagacaactt actgttcgct ttatcgataa atccaatcag
     5581 tgtcgctcct actttcttga tttgttctac tgctttcact acttcttgag ttgtaccggt
     5641 aacagaggac aaaataacga tagaatcttt tgttaatctt ttgtttccag tagtgtaata
     5701 ttctgcagca tgctgtacaa agacaggcaa gttgcttttc ccattcatat aagtgacagc
     5761 ttgcatcgag gaagcatacg ttccaccgat tcctagataa tagacagcgt caaatttttc
     5821 cgcataaagt ttatcgatga tttcttctac ttgcggacgt aaagctagtg cgccttttat
     5881 tgctttgatt tgttcttctt cattgaattt cagcatgatt tccacctctt ataatttact
     5941 tttcaagact gtatcaaagt cttcaaaaat ctttttgttc aagtcaatac ctttagctac
     6001 tgcggtttta taagcgaata gttgaaacgg aacgacaagc gctaatacag acagatattc
     6061 atcacattct attgctaatc caagtgtttt ctcgtttctt gctttttttg tggtaatggt
     6121 gagagtctct ccaacgtacc gactcatata tctttgcaac ttttgcgaac gttccttatt
     6181 tacactttcc ccttcaataa agaatagcaa atgcgaagca tctgcctcca ggtatgggcc
     6241 gtgcatataa gcttctaact caaatccttg tgacggaacg cggattgttt cagtcaattt
     6301 cgtctctgct tctttagctg ttccccagtt tggaccatat ccaatggtga cgaaacgctg
     6361 tgctaaacga aataaacttt gatatccatc aaaaaattct tccgtttttt gaataatcgc
     6421 tggtaaatgg ttgattattt tgcgcaactg ttgcatgtag tcttgttcta tttttttgct
     6481 gattttattt ttacttattc caattcctat ccctaagagt agtaactgta aaacagtcgc
     6541 agaatagcct tttgtaacga agcctactga ttctaccccg gcacctaaat caataatttg
     6601 tgcagcttct aaagcaagtg gactttgcac gtcatttgtg atagcaacaa caggtaaccc
     6661 ttgtttttgt atcattttta ctgcttcaac agtagaagca cttttcccgc tttgtgacac
     6721 agcaatcaca gtatcgattg aaggagaaag ttttccataa ttgaaaaaat aaaaaggttc
     6781 ttgaatatcg acagtaagat caccataaga ttccaatgct ggcttcgctg ctaaacaagc
     6841 gttataagat gaacccgttg ctaagatcag taaattcact gtttttttca tatgttctct
     6901 tgtcgaaaaa tcattctttt tcaagatcgt acttagtact tccttttctt catagatata
     6961 atcttgcatc gtaaacattt tctctgctcc ttttttaaaa aatccctatc cacgaaccaa
     7021 aaattccaaa gaaagctagt ccgatcaaga tcgttaatgg tttcacttct ttcttcaata
     7081 gataaaacac tatgccaaat gctccaagac ataagatatt tggaacgata tcatcaaaaa
     7141 tactttgaac agtcacagca ttttctcctg aaccgatttt taaaggaata ttgatggtga
     7201 tcatcgtggc agtcattgct ccaacgacca tcagtccaat aatagacgcg cctaatgtca
     7261 aactttccat cataccgttt gcctggatct ttttcaaaaa acccgttcct aagcgatatc
     7321 cccaacctgt tgctagatag cgaaacagaa tgtgcggaat attgaaaatc aaaatgaaca
     7381 agatagggcc taaaatattt ccttgcatgg ctaaagaggt acccacacct gtggcaatca
     7441 atcgcaaagt tccccaaaag aaagaatctc ctaaccctgc aagaggtccc atcaaagatg
     7501 ttttgatact atcgatcgca gtcacatcaa attcaggatc atccttattt tcttcctcca
     7561 ttgcagccgt gattcctaaa atcagtgtaa cgatatgtgg ggtcgtgtta aaaaactcta
     7621 gatgccgttt caaagctgct gccatttctt ctttcgactg atataacttt ttcaaaatcg
     7681 gaatcattgc ataaacataa gccatattca tctgacgttc ataattccaa gaaaactcca
     7741 tttgaaaaga acgccaaaat acgtgattta gctctttttt cgttattttg ctgtttttga
     7801 tctcagaatt cttcatcttc ttctacctcc cctgcagttg tttgaagtgg tagttgacgg
     7861 acattcataa tattgactac cacaactgct aaaatcgcac caaaaatagc tactccggtc
     7921 acaggaatct ctaagtaaga agctatagca aaacctaaga aaaaatatgg tgcaacttgt
     7981 ttgttgatca gcaaacgagc aagcatcgca aaaccaagag ctggaatcaa ccctgtagct
     8041 actgacaacc ccttttgaac gaagtcagga atcatattta atagatttcc gatcgtattg
     8101 cttcccacca tataagaaac cattaccaca acaccaagca tcaaggaaag accgataccg
     8161 gcaagcaagt gcatatgctc gactccttta taatttcctt cttctgcata attgtctgct
     8221 ttatggttca taattggaat caaaagccct aagtaaatat ttttcaatat caatgtcaac
     8281 gtagcaatcg gcaaacctag taaaagcgcg gtttctgtac cggctccagc tgtaatagca
     8341 aaagccgtcc ctaaaatccc accggtcaca acatcaggtg gaatcgaagc acctactgag
     8401 aacgatccga taaatgctaa ctctaaagtt gcgcccataa tgatcccagt ttttacatca
     8461 cctaacacga ttcctgtaaa cagccccgtc acaatcggac gagaaagcaa cgatgtccct
     8521 agtgcatatt ctgattgtgc gacaaatgca acaattccta ataagactgc ttgtacaatc
     8581 attgaggttc ttcctttctt acaaaacttc tcttacatcc acttttttat ctgctggtac
     8641 ttgacggatt tctacttcta tttgtttgtc aattaatcgt ttcaagcgtt cctcctcttc
     8701 tggtaaaata tttaccgctt ttccgatgct gcgagtacca ggttttgcct tgatccctcc
     8761 taagttgatg gttttgattt ctggatacgc ttctgccagc ttatatgcat cctcgatcga
     8821 ttccactacg atgaataact tatatttatc tgtcaccccg cttttcaacg cggcaatcga
     8881 gtcatcaatt gatttgatca ctagtttgac tccttgtggc ttggctaatt tgatcgttgt
     8941 ctttcgtatc tcattcgtag gtacatcatc attagcaatc aaaatacagt ctgcgcctat
     9001 tgtttgtgtc catgaaaaag ctacttgtcc gtgtaataac cgatggtcga ctcttgtcaa
     9061 tacaatcatt tttcttcctc ctaaaattcg tcttcttcta ttttctgatt gagccgctcg
     9121 ttacaaaact ggatcatttt ttttgaatca gttagcacct gctggatcaa aacagttgtc
     9181 tctacttcag cgtccaaccg agatactagc tcaatcaata gtggcaaatt caatccggca
     9241 acgagataga catgatcccg attgatatat tgtaagaatt cgttgttgac acttccgcca
     9301 aaaagatccg tcagaacaat gatttcttta tctttgtacc tcataaaaag ctgccttact
     9361 gtttccgcta aatcaaaatt ctcatcagta tagcaatcga atacagtaat agaatgattt
     9421 ttcccataga tcagctctaa tgaatctaat atccctgaag caaattttcc atgggaagct
     9481 aagataattt ctctatccac tttttcatct cctcttttca cttacacata ctaaaaaagc
     9541 aagttgcatg ccaaaattag tggcaacaac ttgcttatgt tcaaaattct tcttctatcg
     9601 agagaaactc tgtatcttca ctcaaaatat catagatata ggcaatctca gaattgggga
     9661 tcatgacact ataatgctct tcaatgacac taaacgcaga tttaatagca gataaccctt
     9721 ctttttgaca ctgcttccat ttctctaatc cttgataggt ttcgattggc atttgtcgga
     9781 tcaatcgttc aatgagacag ctcacatgga catacaacgc taattttttg ggattgctga
     9841 tgcttcgatt caactgcacc tctaattctc tcataaaatg atcgatttct cccatcacct
     9901 tctctgtatc tagaatagtc acggaatcca gtactttttc caatgaaaaa ttacgaatga
     9961 tgcgctgatt gaatatttcc ttctctgaag cattcatagt agtgtccaac cattcagcta
    10021 gtacagacat cttttccccc gagatgaggt cttctaatga aagatagtcg atattggtga
    10081 tatcaggatc tgtggtgcca atcattccta cgacttcgta tatggaaaat aatgggtcac
    10141 tgtttttctt atcttttaat tgctgatatt catatgggat gattttcaac tgacaagccg
    10201 taggtaagct tttttctagc agttcactta agtggaccgc cgtaccgatt ccagtgaagc
    10261 aagtagtaat gagtgctttg ggtttgtttt cctctggata gagcagtcgc cattctggct
    10321 ggatctgtcg aacagtctct tctgctatct cttctaatga tacatttttt tgcaggcatt
    10381 cccctactgt gattgctaaa ggtgtcgtca cattattcaa aatcaaaagc ggtacggata
    10441 actgtttttt aaaaaactga tggatttctt tcagagatcc catgtctact aatatgatca
    10501 atccatttga aacatcgctg ttttcgcaat aatcaaggat ttctgaagca atctgctgtg
    10561 gtgtgacatc tagtggcata tcaaatgatt caaatagatc ctcttgtaat aaacgattta
    10621 ctacatttgc catactgcta gcagttgcat aaccgtgagc agcaatcatc gcttttggca
    10681 gtcgtttttc ttttgaccat ttcgcttttt ttagatagat cgtcagtaaa atccgatcca
    10741 tacttgtgtc ttcaatatct aatttgatct tacttaattc cagaattcgt tggacatagt
    10801 gataactaga tggataagac tgagaaactt gttgttctaa ttctttgatt agttggataa
    10861 tatcatgttc ttccggtaac catttgatag tgcttcgctg atagagatag taactcaagg
    10921 catagacact gtttccatcg aatttgattt gataggcagt ttccatttgt cgaaaggtct
    10981 ctcggataga ttgtgtccaa taaaccagca gctcgtgttt ttcttgtcta ctggtctcaa
    11041 acagcaaaaa atcaaataat tgaatcactt cttgctttaa ttttccttcg caagaatata
    11101 gcagacagtt atttttcttg aaatcaatta ggatttgctc aaaactttta acgattcgct
    11161 gctgttcggg ctgttgcttc tccattaatt gctctaaggt ctggtcttgc gtaatataga
    11221 ttccttgctg tgctgtattt ttagtaatag gctgcgcaag tagttctttt tgcaaatgat
    11281 ggatcgtgac atggatttca ggctgttcac gctgttcagc atatgctttt gctgctgaaa
    11341 ctttgacgat atttttcagc tctcctatgt ttccggataa tttctgactt gttaaaagtg
    11401 acaaagctgg tccactgact gacaaagggc tccccatttt tctttgttct tcgagaaaaa
    11461 aagagtaaat cagttctagc cgttcattac gactacgctg attaagtgca ggtagttcga
    11521 ttcggatagg tattcttcga atgaaagttg ttaagaaatg actagtaatc tcttcagtcg
    11581 tagcaaaaaa gagacgtgtt ttgatccggc gtgacaaatt aggttctccc atacgataaa
    11641 tcacaccttg atcaagatag gtgaacagtt tttcctgacc ttctggtgtt aagcgatgta
    11701 cctcgtctaa aaacaagatc ccaccatctg ctgcttcaaa agcccctttt ttattttctt
    11761 ctgcaccagt gtacgctccc ttaacatgtc caaacaaatt gctagtcaaa agttcaggat
    11821 tatctgcata ttgtgcacaa ttaaaagtga tgaatggtgc agaatcttct agcagatcat
    11881 gaagcagaca atactgatag accaaattaa ctaaataact tttacctgtg ccactttcgc
    11941 ctgtaatcaa aagtggtaat ccgccatctg gatagtaaag agctgttttg atttgttcga
    12001 tactttcttt caaactggcg tcgtatccga tcataaaaga gaataagtct tttggttgcg
    12061 cgaaaaaagg ctgttcctct ttgagatcat cgatagaaaa ataaaaattc gtgcgtaact
    12121 taaaaaattg tttttcaaaa gcttctttgt ggaaataaat cactggtcga gaattgattt
    12181 tgactagtag ttgctcatca tttaactggt tcaaataatg actgactgtg tttcttttta
    12241 ccccaaaatg atcagctaac ctttgtgctg taaagacctc gttcatatgt tctaaatcga
    12301 aaaaggctgt ctggttctct aagtaacata aaatttcctt cttcaacaac ttattcaccc
    12361 ctcctccatc atagaaaacg gattcatttt gtcaataaaa tctttaataa agaattgaaa
    12421 aatatatctt attatagaca gcagcctact tatcccttat aatttttatt ctaaaaaacg
    12481 tacagttcct tccagttttt atactggaat ttcaacatat gaaagaataa ggaggctatg
    12541 actattcaat gaaatcggat aaatataaaa acaggaaaca tacttcctgg aaaacttttc
    12601 gagaaatcat cggatcttat ctcgctgtag ttgtattttt gcctttgctt tatatggcta
    12661 tgacagaaca aactcgtttt attggcgtag atacgcaata tatagccgaa acaggatttt
    12721 tattcatctt tatcttgatt tttgcgttct ataaatcctt ccatacaaaa taagattcac
    12781 agatttcaac tttcctgtct gtttttagct attacaaatc cttatatgtg gtataacaac
    12841 aatttattgg cataatgagg aaaaacgagt tatgatgctt tcagagagta aagaggtact
    12901 gttgtcacca tcctttttat tgattacgta caccactctt agttttttcc cattgattac
    12961 gaaaacaagg tagaaacatt gatgttccta ccttgttttc tttttttatt ttcagatcaa
    13021 aatctcaagt ggcttttgac ttgatgtcgg gaggttgtct tcgttccatc aaacctgata
    13081 gaatctgcgc caaaccatag ttttctccac tattatttat tcctctaaga tagtacccac
    13141 aataaacgaa taaatatttt tctcattttc atactccttc tttgaaaatc aattagattt
    13201 ctctcaaccc acttttttac aaaaattacg tagcaaaaaa gattgcaaga acaaattaaa
    13261 aaagaagtaa atagagtttc atctataa
//
LOCUS       DAYYHS010000076        12972 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000076 DAYYHS010000000
VERSION     DAYYHS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.076"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            215..1501
                     /gene="efmA"
                     /locus_tag="ACHA6A_002221"
     CDS             215..1501
                     /gene="efmA"
                     /locus_tag="ACHA6A_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8970387.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVSNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1639..2238)
                     /locus_tag="ACHA6A_002222"
     CDS             complement(1639..2238)
                     /locus_tag="ACHA6A_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8970388.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2642..3763
                     /gene="mnmA"
                     /locus_tag="ACHA6A_002223"
     CDS             2642..3763
                     /gene="mnmA"
                     /locus_tag="ACHA6A_002223"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8970389.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            4009..5430
                     /gene="arcD"
                     /locus_tag="ACHA6A_002224"
     CDS             4009..5430
                     /gene="arcD"
                     /locus_tag="ACHA6A_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8970390.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5444..6562
                     /locus_tag="ACHA6A_002225"
     CDS             5444..6562
                     /locus_tag="ACHA6A_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8970391.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6622..7914)
                     /locus_tag="ACHA6A_002226"
     CDS             complement(6622..7914)
                     /locus_tag="ACHA6A_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8970392.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            8064..8624
                     /locus_tag="ACHA6A_002227"
     CDS             8064..8624
                     /locus_tag="ACHA6A_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8970393.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8753..9130
                     /locus_tag="ACHA6A_002228"
     CDS             8753..9130
                     /locus_tag="ACHA6A_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8970394.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            9063..11135
                     /locus_tag="ACHA6A_002229"
     CDS             9063..11135
                     /locus_tag="ACHA6A_002229"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8970395.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11346..12293)
                     /locus_tag="ACHA6A_002230"
     CDS             complement(11346..12293)
                     /locus_tag="ACHA6A_002230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8970396.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12539..>12972
                     /locus_tag="ACHA6A_002231"
     CDS             12539..>12972
                     /locus_tag="ACHA6A_002231"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8970397.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         4178 a         2345 c         2759 g         3690 t
ORIGIN      
        1 tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca catgttgtta
       61 tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg aaacgattgt
      121 tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg ttgtgcaaac
      181 gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt cggtcgtttt
      241 aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt caggaatcac
      301 tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg gttcggcgac
      361 aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa gcccatttgt
      421 aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg atattattgt
      481 agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct tcccgatttg
      541 gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa tgccaacgat
      601 ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga atggacagtt
      661 aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg ctttgttttc
      721 agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt ttggtgtcgg
      781 cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag ttccgcttac
      841 tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag ggttatggta
      901 cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca gtctgtatcc
      961 attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag tggaagtagt
     1021 ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa catggaaaga
     1081 ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg gtgcaagcgg
     1141 attggtttca aacgatagtc aagggttctt gtactttctt atcttgaatg caggagcagg
     1201 ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga gttatgaatc
     1261 gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg caggtccaat
     1321 tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa tgttcttgtt
     1381 ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag ctgcccgaaa
     1441 ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa aagacgaata
     1501 gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc ctaatgttcc
     1561 tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt gtttaatact
     1621 tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat agatttcttt
     1681 gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga gttctctttc
     1741 cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat gatggatatg
     1801 tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat gagtcaagta
     1861 atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa atttgggact
     1921 tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc gactgtcatg
     1981 gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca aaatttctgc
     2041 atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa catggtcggt
     2101 ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat ccacatttgc
     2161 aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa agtgcatatc
     2221 actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa acaaaatcac
     2281 ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg tgcagcttta
     2341 agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta ttccttattg
     2401 ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc tcaaaagatg
     2461 agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag aacagtattt
     2521 ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat gtaaaatact
     2581 gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg atggtgatat
     2641 catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg tcgactcatc
     2701 tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat tcatgaaaaa
     2761 ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata aggatgtagc
     2821 gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa aagaatattg
     2881 ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac ctaacccaga
     2941 cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa tggatcttgg
     3001 cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa acggcgtggt
     3061 ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta gccaattatc
     3121 acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat cagaagtacg
     3181 ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa cgggaatctg
     3241 ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag caaaaaaagg
     3301 aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga tgtattatac
     3361 gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag aaccctggtt
     3421 tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt tccatcatcc
     3481 tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga atgaaccaat
     3541 gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg ttcctgtgac
     3601 agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg tacgtgcaat
     3661 cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg gtggtttgat
     3721 cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat taaatgaatc
     3781 aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag ctgttttttt
     3841 gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa agttcataat
     3901 atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa aatagaaggg
     3961 aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat ggaaaagaaa
     4021 tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc aatcggaagc
     4081 ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc agcgatcctt
     4141 tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa tcatttagga
     4201 gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc atttggtaaa
     4261 ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg aaatgttgct
     4321 tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa aggtggacaa
     4381 aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt attagtaaac
     4441 aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa attagtgcca
     4501 ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt cactgctgat
     4561 ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg aacgatctgg
     4621 ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat cgaaggcgca
     4681 agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc gatcattggt
     4741 ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg cgtcttatct
     4801 caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa aggaatcgtg
     4861 ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat tggttcttgg
     4921 ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga cggtctgttg
     4981 ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt agttgttact
     5041 gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc ctatcaattt
     5101 gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg gatctatcaa
     5161 gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg gattgctgta
     5221 gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt atgtagtatt
     5281 gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa acatcaaatt
     5341 acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct ttcaatcggg
     5401 ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa tcgcaaattt
     5461 cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata ttgcacaaag
     5521 ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta gcgtaagtaa
     5581 atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag gatcagatgt
     5641 tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca tcttacaaac
     5701 gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag caggagacca
     5761 tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat ctttaggtgc
     5821 cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt tagaacagct
     5881 agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa acaatcctac
     5941 aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc gtacagtaga
     6001 tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg aattcttgtc
     6061 gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa cctactccat
     6121 tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag tttttcgaaa
     6181 gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg ctgtacatgc
     6241 attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag agaaccttgt
     6301 gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc ccaattacgt
     6361 ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt gtatcaattt
     6421 attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac cgaagcatgc
     6481 ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc tcttatctga
     6541 gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga caactgttca
     6601 agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg aagctatgga
     6661 gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg tattgtgtcg
     6721 tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc cgcgataacc
     6781 aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag ttgcttggaa
     6841 agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt ttgtgatcaa
     6901 tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac gtaaggcagc
     6961 taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg caaaacccaa
     7021 accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa atcttggagc
     7081 ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca acgtaacatt
     7141 cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa tggaattgtt
     7201 ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg gatatttcaa
     7261 gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga caaatttttc
     7321 atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg taatgaaaaa
     7381 ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc caagtaatgt
     7441 tcctgtaaca gaaccagttg ctaaggttcc aagaacttga ccactttttt cttttggcgt
     7501 cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc cttgaagcag
     7561 acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa tgacgattgc
     7621 cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg ctaattttcc
     7681 ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca atccgctcca
     7741 aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg acataaaagg
     7801 catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga caaacaagtt
     7861 ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag ccaattagac
     7921 tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat atgtagttta
     7981 tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag tgaaggattt
     8041 attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag aaaatcatcg
     8101 actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt cagcatttag
     8161 ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat attcgtgatg
     8221 tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg cgacgggctc
     8281 ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat cgatcctttg
     8341 ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc aatgaagctg
     8401 ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt gaggcttttc
     8461 aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg atccacggtt
     8521 atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat ccagaagaca
     8581 gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc agattcattt
     8641 gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc ttgcaacttt
     8701 cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga gtatgtatca
     8761 agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact gggaagaaga
     8821 tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt acgaaaaaca
     8881 atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact ttctcagcgc
     8941 cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt tacagcaata
     9001 caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca gaaaggaatt
     9061 ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta agcaaggtgc
     9121 ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc gttacttggg
     9181 cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa tggcagagct
     9241 ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca cttcctaaaa
     9301 caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc tcagaagcag
     9361 tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc ttagagtatg
     9421 tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat aaagcaatca
     9481 aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat gaatctgctc
     9541 ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc ttaactgtga
     9601 agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca ttagtgagag
     9661 accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc acgttgcaag
     9721 ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa acacaagaag
     9781 aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca gaagtccaag
     9841 aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc caaagagaag
     9901 ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa agtttgtatg
     9961 agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca aatgacatga
    10021 aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag gtcaagcagt
    10081 atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt aaagtgaccg
    10141 atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta agtaatgaag
    10201 agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct caaccccata
    10261 aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt tttcggcaaa
    10321 atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa acagtcaaat
    10381 gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta tctgcaccgc
    10441 ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt ctgggaaccc
    10501 cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg gaacgtaccc
    10561 ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta aatccctctt
    10621 tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt gctgcaggaa
    10681 agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc ttggtgaatg
    10741 agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag aaatgcccag
    10801 aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat gtctgcacca
    10861 attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat cgatgcccgc
    10921 aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc cgttgtaaat
    10981 acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa atgaccaaac
    11041 atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa gaaagcccat
    11101 tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag gttgtgatag
    11161 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    11221 aaatttcggc ttatttccga agaagctact tctggaacac catttattcg gaaaaaagga
    11281 ctgtgacaag acttttgtca cagtcctttt tcatttaaaa aaatgtttac ttcatttgac
    11341 gtatattatt ggtgaggctt caattcattt ggcttcactt tttctgtttc tcttatgata
    11401 taaacaggac gctgtttcgt ttctaagaag attttcccaa tatatttccc gatgattcca
    11461 agacataaca attggattcc accaatgaac aggaagatcg agaccatgga aggccatcct
    11521 gatgtaggat caccaaacaa caatgcccgc aaaataatga acaaaattgc aataccagaa
    11581 ccgacacaag acaaagcgcc gataaatgaa gcaatcgtta acggtgcatc cgagaagttc
    11641 acgataccat caatcgagta gttgaataat ttccagaagg accaagatgt ttcgccagcc
    11701 acacgttcgc ggttttcata agaaatatat tctgttttaa atccgaccca gctaaataat
    11761 ccttttgaaa atcggttgta ttctgttaat tctaggacgg catccaccat ttgacgagtc
    11821 atcaagcgga aatcacgagc gccatcgacc atttctgtat cgctgatttt attgatcaac
    11881 tggtagaatt ttttcgcaaa gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc
    11941 cgggtgccga cacaatcgat atcagaagta gtgatccggt tgatcatctc aggaagcagt
    12001 tcgggcggat cttgcagatc aacatccatc acggtcacta aatctcctgt agaagcttgc
    12061 aatccagcgt acagagccgc ttctttccca aaatttctgg aaaatgagac aaagcggaca
    12121 taaggataag ctgaggctag cttcttgatt gctgctaacg taccgtcttt tgatccgtcg
    12181 tcaataaaca aatattcgaa ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa
    12241 agaggaacag ttttttcttc attaaaacat gggacaacaa ttgatacttt catcgtttct
    12301 atcctttcaa tcgtatgttt atatatggtt tacattcaaa atttaataaa ccttaaataa
    12361 cacctttaaa atttgatttt acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt
    12421 tagtttacca tgtttcctcg gtttatcaaa cttttaagaa caatttttat tagttggcaa
    12481 gtagttttgg atagagaaaa aggaggaagt tccagtctat tgactaggag caaacaaaat
    12541 gaaaatacac acacctatcg gctttttatg gttagatgca gatgaatccg gattgactgc
    12601 tgtatctttt cagccaattt cagaaagcga atcagcgaat cagcgaattt taacgcgcac
    12661 acaagaacag tttgaagaat attttgcggg aaaaagaaaa gaatttgatg ttcctttatc
    12721 tataaagaga ggtacgcctt ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta
    12781 tggagaaatc cgaacgtata aagaagtagc cgttgctgtt gatagtccga aagctgtgcg
    12841 agcgattggg caggcaaatc gaaccaatcc attgccaatc gttattcctt gtcatcgagt
    12901 gatcgggcaa aatggaaaac tgacaggata tatgggaaac gcaatagatg gggttgaaat
    12961 caaacgccaa tt
//
LOCUS       DAYYHS010000077        12929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000077 DAYYHS010000000
VERSION     DAYYHS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.077"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1561)
                     /locus_tag="ACHA6A_002232"
     CDS             complement(56..1561)
                     /locus_tag="ACHA6A_002232"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8970398.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="ACHA6A_002233"
     CDS             complement(1653..2243)
                     /locus_tag="ACHA6A_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8970399.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="ACHA6A_002234"
     CDS             2422..4068
                     /locus_tag="ACHA6A_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8970400.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPRY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="ACHA6A_002235"
     CDS             4117..4836
                     /locus_tag="ACHA6A_002235"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8970401.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..6262
                     /locus_tag="ACHA6A_002236"
     CDS             5315..6262
                     /locus_tag="ACHA6A_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8970402.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6274..7194
                     /locus_tag="ACHA6A_002237"
     CDS             6274..7194
                     /locus_tag="ACHA6A_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970403.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7235..8692
                     /locus_tag="ACHA6A_002238"
     CDS             7235..8692
                     /locus_tag="ACHA6A_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8970404.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9037..9651
                     /locus_tag="ACHA6A_002239"
     CDS             9037..9651
                     /locus_tag="ACHA6A_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8970405.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9644..11368
                     /locus_tag="ACHA6A_002240"
     CDS             9644..11368
                     /locus_tag="ACHA6A_002240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8970406.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11365..12816
                     /locus_tag="ACHA6A_002241"
     CDS             11365..12816
                     /locus_tag="ACHA6A_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8970407.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         4193 a         2238 c         2591 g         3907 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccccgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt
     5581 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
     5641 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
     5701 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
     5761 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
     5821 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
     5881 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
     5941 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
     6001 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
     6061 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
     6121 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
     6181 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
     6241 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
     6301 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
     6361 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
     6421 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
     6481 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
     6541 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     6601 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttaattac gatgttgttc
     6661 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     6721 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     6781 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     6841 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     6901 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     6961 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     7021 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     7081 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     7141 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     7201 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     7261 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     7321 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     7381 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     7441 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     7501 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     7561 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     7621 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     7681 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     7741 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     7801 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     7861 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     7921 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     7981 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     8041 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     8101 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     8161 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     8221 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     8281 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     8341 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     8401 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     8461 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     8521 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     8581 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     8641 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     8701 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     8761 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     8821 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     8881 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     8941 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     9001 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     9061 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     9121 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     9181 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     9241 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     9301 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     9361 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     9421 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     9481 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     9541 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     9601 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     9661 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     9721 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     9781 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     9841 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     9901 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     9961 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
    10021 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
    10081 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
    10141 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
    10201 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
    10261 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
    10321 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
    10381 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
    10441 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
    10501 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
    10561 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
    10621 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
    10681 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
    10741 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
    10801 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
    10861 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
    10921 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
    10981 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
    11041 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
    11101 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
    11161 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
    11221 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
    11281 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
    11341 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
    11401 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
    11461 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
    11521 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
    11581 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
    11641 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
    11701 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
    11761 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
    11821 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
    11881 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
    11941 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
    12001 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
    12061 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
    12121 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
    12181 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
    12241 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
    12301 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
    12361 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
    12421 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
    12481 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
    12541 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
    12601 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
    12661 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
    12721 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
    12781 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaaagccaat gtaatgaaat
    12841 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
    12901 cagctttttc atgacctctc gataaacgg
//
LOCUS       DAYYHS010000078        12731 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000078 DAYYHS010000000
VERSION     DAYYHS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.078"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHA6A_002242"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHA6A_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8970408.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACHA6A_002243"
     CDS             complement(324..893)
                     /locus_tag="ACHA6A_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8970409.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACHA6A_002244"
     CDS             1079..1981
                     /locus_tag="ACHA6A_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8970410.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHA6A_002245"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHA6A_002245"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8970411.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHA6A_002246"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHA6A_002246"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8970412.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHA6A_002247"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHA6A_002247"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8970413.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8545..8730
                     /locus_tag="ACHA6A_002248"
     CDS             8545..8730
                     /locus_tag="ACHA6A_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8970414.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8792..9988)
                     /locus_tag="ACHA6A_002249"
     CDS             complement(8792..9988)
                     /locus_tag="ACHA6A_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8970415.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10158..11594)
                     /locus_tag="ACHA6A_002250"
     CDS             complement(10158..11594)
                     /locus_tag="ACHA6A_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8970416.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11887..12696
                     /locus_tag="ACHA6A_002251"
     CDS             11887..12696
                     /locus_tag="ACHA6A_002251"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8970417.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2672 c         2249 g         4008 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg taatctgtag gaatgagttc tttcgcaaga
     8461 actcaaattt tattgagatt tggtctcgtt tctttacatt caaagaaaat ctggtaaaat
     8521 taagatagaa aagaggtgtt ctttatgcga tatatcgtta cattattctg ggctgtcgtt
     8581 ctaggtcaag tagtcggtta tattggtgct gcattaacaa gcggaacata tgattttact
     8641 ttgacaacaa tcatttcatt cattgcgggt gtcattatcc ttttgattgg tgcagttgcg
     8701 ccaagaaaag aaacctctgc ccattcgtaa aaaaacagcc aagagcttga gatttctcaa
     8761 gctctttttt attttctact cctcaccacc attataatct gctgcgaaga actttacgaa
     8821 agttgaataa agaaagaaga atatttgaca gaacaaacaa acttgtcaca ataaaaacag
     8881 cactgtaatt ataagcatgc gcgactgtcg atcctagcat gggacctaaa acttgtccaa
     8941 agttgctaaa catctgattg tagctataga ttcggctgac tccttcttga ggcgtaatct
     9001 tactgatcag tgtattgaca gaaggcatca aagcgccagt tgaaaagcct aaaataaagc
     9061 gaagtatacc taattggagc ggtgtagtga cgaatgccat aggtaaataa cagcaaaatg
     9121 ataaaatgag tcctccaatt agaacttttt gattcccaat cttatcccct agttttccta
     9181 gtttcggtga agaaaagaca gcggaaacac cagctatcga aacaatcaag ccgctgacaa
     9241 aaaggatatt ttctgtactt ccgctcaatt cacgaatata taaggtcaaa atagggctga
     9301 tcgtagtgat tcctatctgt aaaatcaagg tcgtaatgaa gagaccgatc aaaatagaga
     9361 gatgatccat ttttgaaaaa atttctttgg tgcttatcag atcttttttc tcgattggat
     9421 ggaaatcttc tttgactaag aaaatggtca ataccgttgt gatcatcaac aaagcccctg
     9481 tgatcaagaa tacattttcc ataccaaacc actgagccag tgcaccgccc attgaaggtc
     9541 cgatcagatt tccagcaatc gctccggtag acaatgttcc taacgcccat ccgctctttt
     9601 ctctaggtgc ctgtgaagca atcatcgctg tggcatttgg aatatatcca gacaaaatcc
     9661 cattccaaaa ccgcatgatc aaaagccaat acgcgtttgg tacaaaagcc aaagatccca
     9721 ttgttaatgt catccctgct gctgcacgga tcatcatgag ttttcggcct tttcgatcag
     9781 ctagatttcc ccagattggt gcaaccacgg cagatgcaaa agcagtcaca gagattgcta
     9841 gtcccgaaaa caattcgatt tgatcttttg gtgttcctag ttgctcaaca taaactggta
     9901 tgaatggcat aactaaacta atacttgcac cagtgaaaaa acagccaatc cacgcaatca
     9961 ctaaattttt tctccaatta atctccatct atcatccctc tttcgtctct tttatcatac
    10021 ctattttcga ataaacaagc aacgatctcg ccagtaaaaa gagaaaaatc ccgcaaaggt
    10081 cattttatat gtaacgttct ttttatgtaa aacaaaaaga gatcagaaca aatactgttc
    10141 caatcccttc tctttagtta aattgttgct agaatgatga agttgataat gaataaaata
    10201 tcaacgatcc acaaagcaac agaaacttga ttgtattttc ctttgattac tttcacaatt
    10261 gtatagaaaa taaatcctgc tgcaatccca taggaaatgc tatagcacaa tcccataaag
    10321 atcgatgcaa agaaggccgg taaagcttct tccatatcta gccatttgat gtctgcaaaa
    10381 gaagccatca tcattactcc aactaaaatc aatgctggcg cagttgcttg atttggtaca
    10441 atggcgatca gtggagagaa taaactactt aatgcgaata gcactgccac tactactgaa
    10501 gtcaaaccag tacgtccacc ggccccgatc ccagctgcag attcgacata agttgttgta
    10561 ttcgatgtac caaagatagc ccccacagaa gtagcgatcg catcggcaaa tagtgcttta
    10621 tccatttttg ttttgaaccc acgaccatct tccaacgcga tctcgtcttc ttttgagaaa
    10681 atacctgtgc ggcgtcctgt accgataaat gttccgatcg tatcgaaagt atcagataaa
    10741 ctgaatgcga tgatcgtcat caatacttga ggaatcttgg aagaatcact gaataaagat
    10801 tgcatgcctt cagaaccaaa tgctgcaccg aaagttgttc ctaattcttt aaatgaatta
    10861 cctaacgaat tcgtttgcca atcaatggaa cttaaatcaa caacacccat aaaaatacca
    10921 aggactgtgg tcgctacgat accgataaga atagccccac ggacattttt tacaactaaa
    10981 atggctgtca gtaccaaacc aatcacagcc aataagatag gagcgttgtc aaagttggct
    11041 aacgcaggga cgattccacc atttatcgta acatttgtcg cttttccgcc ttcaacgaca
    11101 gagctcgaaa tagctgactg gtctgctgaa aaagataaga atcctgcatt tttgattccg
    11161 acatacgcaa cgaaaatacc gatcccgcca ccaatcgcat gttgaagact ttcgggaatc
    11221 gcccgaataa tcatttttcg aatatttgtg acagtaataa aaatattgat taatccgcaa
    11281 ataaacacca ttgccaaggc ttgttgccaa gaataaccaa gcccaaaaac gaccgtaaaa
    11341 gtgaaaaatg cgttcaatcc cataccaggt gcctgagcat atggtacatt tgcgaacaat
    11401 cccattacca gcgtaccaat aatcgatgca atgattgtcg ctagaaatac tgcttggaag
    11461 ggcatacctg atgctgacaa aatcgtcgga tttacgaata agatataact catggcaaag
    11521 aatgtcgtta ctcccgccat tatttcagtt gatacatttg tattgttttt ctttaaaccg
    11581 aaaaactttt ccataaaaag ttccactccc tgaatttttt tctgatggaa aactataata
    11641 ataaaaaata agtaaaatag ggcaaatcca tcgaacaatt tcaaattata tagacgtttc
    11701 attcgctttt caacgttttt ttaacaaaaa aagcgaaaat aatacttaac gttcgtgttt
    11761 agcttatgtt tagctattta tatagccata actgtagttt ttgttaggaa tagctgattg
    11821 ctcttgttaa ttacctaaaa tatgctagac taaaacaaat gctagttgag gaaggaagga
    11881 cccttattga ataaaaaatt aattgccatc gatctggacg gaactaccct caatcaagat
    11941 tcgatgatta catctaaaac aaaagagact ctaaaaaaag caatcaatgc cggtcatcat
    12001 gtcagtattg ctacaggacg cccattccga atgagccatc agttttacca acaattagaa
    12061 ttaacaacac cgatggttaa ttttaacggc gcattagtac acatccctaa tcagcactgg
    12121 gatggtgaaa gagaaacgtt gatcaatcgt gaaatcgctt ttgaaatctt atcgcaaaaa
    12181 aagcagctaa atttagattt tatcgctgct gaaaatcgtg atactttttt tattgacagt
    12241 tttgatttct ttgatgaaaa aatttttgct tcttctcgtc caggagagaa gaatttgctt
    12301 tctcctaaaa atttgacgac aaatcctaca tctttgcttg tccgaacaga caaacgtttt
    12361 gcaggagctg tatctgctga attaacacgt caatttggca gttatgtcga tgttcggaca
    12421 tggggtggac ctactgccat cttagagatt gtttcaaaag gaatccaaaa agcaaaaggc
    12481 gtgcaagaga ttgctaacta cctgtctatc gatcaaaaag acgtcattgc ctttggtgac
    12541 gagcataatg atttggaatt gttggattat gcaggttggg gagtagctat ggctaacggt
    12601 actgaccaac tcaaaggcat tgcaaatgat gtcactcctt tgagcaatca ggaagacggt
    12661 ttagctgttt atctggaaaa actacttaaa ctataataga gaattagatt ccacaaaaaa
    12721 gaggttgtga c
//
LOCUS       DAYYHS010000079        12683 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000079 DAYYHS010000000
VERSION     DAYYHS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.079"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2145
                     /gene="acm"
                     /locus_tag="ACHA6A_002252"
     CDS             <1..2145
                     /gene="acm"
                     /locus_tag="ACHA6A_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8970418.1"
                     /translation="ILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDG
                     GNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKD
                     SAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPAS
                     GSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEI
                     NQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKT
                     IIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYI
                     NDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNW
                     IDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFR
                     ASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGY
                     QKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEK
                     DDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHI
                     TPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILP
                     KTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2417..2902)
                     /locus_tag="ACHA6A_002253"
     CDS             complement(2417..2902)
                     /locus_tag="ACHA6A_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8970419.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    2992..3337
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3382..4023
                     /locus_tag="ACHA6A_002254"
     CDS             3382..4023
                     /locus_tag="ACHA6A_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8970420.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4042..4677
                     /locus_tag="ACHA6A_002255"
     CDS             4042..4677
                     /locus_tag="ACHA6A_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8970421.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4695..5510
                     /locus_tag="ACHA6A_002256"
     CDS             4695..5510
                     /locus_tag="ACHA6A_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8970422.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5829..7265
                     /locus_tag="ACHA6A_002257"
     CDS             5829..7265
                     /locus_tag="ACHA6A_002257"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8970423.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7454..9886
                     /locus_tag="ACHA6A_002258"
     CDS             7454..9886
                     /locus_tag="ACHA6A_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8970424.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10116..11183
                     /gene="rlmN"
                     /locus_tag="ACHA6A_002259"
     CDS             10116..11183
                     /gene="rlmN"
                     /locus_tag="ACHA6A_002259"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8970425.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11173..11379
                     /locus_tag="ACHA6A_002260"
     CDS             11173..11379
                     /locus_tag="ACHA6A_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970426.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11376..12479)
                     /locus_tag="ACHA6A_002261"
     CDS             complement(11376..12479)
                     /locus_tag="ACHA6A_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8970427.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4326 a         2145 c         2698 g         3514 t
ORIGIN      
        1 attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt tcatgtctat
       61 gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat
      121 attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc
      181 caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg cggtgaagga
      241 ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt aacggtcaga
      301 aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc agtagaaggt
      361 tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga aaatacggga
      421 ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa gccagcttct
      481 ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga agatactaag
      541 cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca accagttaaa
      601 atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga aattaatcaa
      661 atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca atttttacaa
      721 gatttcccca gcgctacatt taatttttca gtaactgata attacattga aataactata
      781 ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat catagaaaac
      841 ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt taataaacct
      901 gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc tggtggagtc
      961 aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac agagattggt
     1021 atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca aggacagtct
     1081 agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct gcaagtagga
     1141 gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc actaagttca
     1201 aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt acctatttat
     1261 aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg aaccactcca
     1321 agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga accagttcct
     1381 gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga agatatccaa
     1441 cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga
     1501 aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag
     1561 aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga gaaccaagac
     1621 ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc gatccaacac
     1681 aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc aaaatacaga
     1741 gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata cagtacgacg
     1801 attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat atctatacca
     1861 ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga gaaaattaga
     1921 aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag cgaacaaaga
     1981 agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc aaaaagcatt
     2041 cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt attgatagta
     2101 actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga atataatatt
     2161 ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttataatc gaaaaaagaa
     2221 gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg gctaactatg
     2281 gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt tgattggcca
     2341 aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat tgggaaataa
     2401 gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag taaacaagta
     2461 tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt ggatgaaagc
     2521 ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt ttctctttgt
     2581 cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg tcaggaagct
     2641 gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt tgttttaaat
     2701 cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt cttgatttca
     2761 ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg actgacgtaa
     2821 tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt tcataagctt
     2881 cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt gtatcataaa
     2941 aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa acaaatgaca
     3001 tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg gaaagaggca
     3061 gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt aggaaaatcc
     3121 ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg cttttccgtg
     3181 aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt cggatcaaag
     3241 gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc aaggaaaaaa
     3301 taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga ggattttttt
     3361 attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca attattagta
     3421 ggagcactca caacattgaa attattttta tttaccttga ttggctcatt accattaggg
     3481 attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt gcttcaaaca
     3541 tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat tttttatggg
     3601 ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc ctttatctta
     3661 aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat tcctcaaggt
     3721 caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac gaaaatcgtc
     3781 atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat caacttggtc
     3841 aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc tgggaaaatc
     3901 gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat ctatcttaca
     3961 ttgactggta ttctaacaat cgtaatgaaa caagttgaag gaaaattaaa ttattaccgc
     4021 taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc atttggtgaa
     4081 aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt agctatcgta
     4141 ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt agaaagttct
     4201 gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct taaaatgaaa
     4261 gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct gaatattttt
     4321 gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga atacaccaaa
     4381 aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat ctatccttat
     4441 cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc tatgaatcca
     4501 cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg ccagcaagtc
     4561 gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt cacccatgat
     4621 ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat tcattaagaa
     4681 aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca ggtcttcttc
     4741 tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact atcaacgaag
     4801 agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt agagataaag
     4861 acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa caatatggga
     4921 ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg aacaatggaa
     4981 cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag aaagtgcaat
     5041 ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa tcaggaatca
     5101 caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca tctggttatg
     5161 atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa gatgctgttt
     5221 tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc gatggtctgc
     5281 tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta gaagacttca
     5341 acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc aagtcagata
     5401 gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca ggtgaattcc
     5461 aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa caaatagggg
     5521 actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc tcctgccaac
     5581 tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg tgttcaaaag
     5641 ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt ttcacaaaat
     5701 ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata attttatttt
     5761 gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta taggaaagga
     5821 agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg tggggctctg
     5881 cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga gaatcagtct
     5941 gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt gatttggcag
     6001 tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa ggtctgaaag
     6061 cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga gaagtcaatc
     6121 aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac gatattgaac
     6181 ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa tataatggtt
     6241 gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta tttgaggcat
     6301 tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc acttcattag
     6361 gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg tatgaagtta
     6421 accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag ttagaaatgc
     6481 ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac agtaatcctg
     6541 aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg atggatgtct
     6601 atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa ggatgggcac
     6661 caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat tttcttggga
     6721 tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt ggtgctggaa
     6781 aaatgaatac gacaggaaaa aaaagttcat cagaagaaac tggagttcct ggaatgtata
     6841 aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt gacccagaag
     6901 gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta ctgattacgg
     6961 aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat gacgactatc
     7021 gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg gatggagcag
     7081 aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg aacggttatc
     7141 aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga tctttggaac
     7201 gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat ggagagaacc
     7261 tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc ttttcttgat
     7321 aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca
     7381 gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa cttgatgaaa
     7441 tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa
     7501 aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg ggtttgcctt
     7561 gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat tgattgcaaa
     7621 cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat tagccgtatt
     7681 aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc gacgctggtt
     7741 tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt tatttggcaa
     7801 actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag ccagtgatat
     7861 aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa tcctttatca
     7921 ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc tattactggc
     7981 aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg gtatccaatc
     8041 aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag ctcgaaagca
     8101 agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc
     8161 tgcaaaagaa gctgcagaat atgagaagaa aaaagcggtg agccagcaaa gggaaaatac
     8221 tgctgatgaa tggcaggaac cagaaaatac agagccggag cagctaagtt ttgttccaat
     8281 cgacagtttt caagaaaata tccatcctgc gaatctagag aaaccagtac cagatacacc
     8341 aaaacagacc aatacagcag aaggtttttc agatgagttg ccagaggatg acggtacctc
     8401 attagaattt gagattgaag cagaacagga aaatcaagac tatgaattac cgacagttga
     8461 tttgcttgat tctataccaa ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa
     8521 tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc
     8581 cagcttagga ccatccgtca caaaattcga agtccagccg gctgtagggg taaaagtaag
     8641 taaaattgtg aacttaacag atgatatcgc gcttgcttta gcggcgaaag atgtccggat
     8701 ggaagcaccg attccaggta aatcactgat tggtattgaa gtaccaaatg gaaagatcag
     8761 tatggtttcc tttagagaaa tcatcgaagc acaaccaaac cacccagata aattactaga
     8821 agtaccttta ggacgtgatg tttctggacg ggtacaaacg gctgatcttt cgaagatgcc
     8881 tcacttactc gtggctggtt cgactggtag tgggaagtca gtagcaatta atgggattat
     8941 cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa
     9001 aatggttgaa ttgaatatgt acaacggcat acctcatctg ttgacgccgg tcgtgacgaa
     9061 ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag aacgatatga
     9121 aaaattcgcg gcaaccggag taagaaatat ttctggttac aatgaatttg ttcaacagaa
     9181 aaacttggaa aacggaacga aacatccaac cttaccattc atcgtagtga tcgtagatga
     9241 attagctgat ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc
     9301 gcaaatggct cgggcagcgg ggattcatat gatcctagct actcaacgtc caagtgtgga
     9361 tgttatcaca ggaatcatca aagcaaatgt accttccaga atggcatttg cggtatcaag
     9421 cggtacagat tctcggacga tcattgattc taatggggca gaaaagctct tagggcgagg
     9481 agatatgcta ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat
     9541 ttctgaccac gaagtagaac gggtcgttca atttgttaca gatcagcaag aagctcatta
     9601 tgaagagaaa atgatgccga cagatgaagt tgaaacgaca ggtgctccag aacagccaca
     9661 agatgagctg tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg caagtatttc
     9721 ccttctccaa agaagatttc ggatcggtta caaccgagca gcacgtttag tagatgaact
     9781 ggaagctcat ggagttgttg gcccgtcaga aggaagcaag cctcggaaag tattcattga
     9841 gcaggaagaa acatcagaaa cgttgccgga cgataatgac atgtaaaact tgtgtgatga
     9901 agggttgaaa aacgaataac caataaaaag acaaggcatg atgataccaa aagaaaaaat
     9961 ggtttttttc catcttttct aatacggtat gatgtcttgt ctttttagtt tttgtgagat
    10021 taaagttcta ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc atgtatagta
    10081 tgtagcgaaa ctttgagaaa aagagggaaa atactttgga aaaacagtcc atttatggct
    10141 tgacgaatga agaattaatc aattggttta tagaaaacgg cgagaaaaaa ttccgagctg
    10201 cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc
    10261 tatcgaaaca attgatcgaa aaattatcgg agcattttat catcaatcct ctaaaacaga
    10321 tggtcgtcca agaagcttcg gatggtacag taaaatattt gtttgaactc cctgataaaa
    10381 acatgattga gactgtactg atgagacaag aatatggctt gtcagtttgt gtgacgacac
    10441 aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg
    10501 atctgactgc tggagaaatt gttgcacaga tcatgatggt ccaacattat tttgatgagc
    10561 gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag ccatttgata
    10621 attacgacaa tgtaatggac tttttgcata tcatcaatga tgccaaagga ttggcaattg
    10681 gtgctcggca cattacagtt tccactagtg gattagcaca caaaatcaag gaattcgcta
    10741 ataacggctt gcaagtcaat ctggctattt ccttgcacgc gccgaataat gaggtgcgaa
    10801 catcgatcat gcggatcaac cgaagtttcc caattgagaa attgatggaa gctgtggatg
    10861 agtatttaga gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa
    10921 atgatagacc agagcatgcg caacaattag cagatctatt aaaagataag aaaaaattag
    10981 catatgtcaa cttgattcca tataatccag tcagtgaaca tgatcaatat tctcgcagtc
    11041 caaaagctga tgtattgaga ttttatgatg tattgaagaa aaacggcgta aactgtgtga
    11101 tacgtaaaga acatggtaca gatattgatg cagcttgcgg acaattgcga agcaagcaaa
    11161 tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt tttcaattac cataaaagga
    11221 gcagttttct taattaagga actgttcaac ttaaataaga gaaaagattc tatagcgaaa
    11281 gctataaagc tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg
    11341 atgacagcct gcttgatggg ggttttagca attatttaac tgattttctt atatccttgc
    11401 tggctttctt gtgtagcatg ataagtttct acgtcaagaa tggcctgttt aaatttttct
    11461 cgggtgattt cataaggcac atgtttaata tcatttgttg ccatggcttt atctaagatt
    11521 gcttctaaat cggtcgtgac attgagattc atgtcggtta acgatactgg aagttggttc
    11581 tttttcataa acacatagat tttatcaaac aaatcgaact gctgatccat catcaataag
    11641 gtcagtacac cataactgac agatgctccg tgaaggtatc cctccatttt ttctagaaca
    11701 gtcgtaccgt aatagaatga gtgtgccata gcagaattat agttattgtt aactaaaaca
    11761 gaaaccaatc cggtagtaac aatgatatcc gaaacgactt gttcaattgc atatgaggga
    11821 cgatcgtcgg cactatcttt caatgcttgc gtaccataag tcaagagacg atcaatgcac
    11881 atttttccga tttggattcc taatctatcc gtgtagccta gatcatcgcc acgagcagaa
    11941 aaagtagatt cgacttcttt ggataaagca tcgccaatac ctgcccataa gtatttatca
    12001 ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag
    12061 aagagtccgt ccattacgcc ttcttcgtta tagatgacac ataccgcagt gacgggcgaa
    12121 cagttcgaac caatagtggg aaaggtgaag actggtttag ctagtttttc accaatgact
    12181 tttacagtat cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc tttttggaca
    12241 ctttcatgct ggatcaactg ttcaatattt ccataagttg aattaccccc gtaccagaga
    12301 ctggcgacaa cctctatttc ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa
    12361 agtgcagtct tccctccaat cagcactact tttgtcccat attctttgac gattccattt
    12421 gctttttcat acgcatctgt tccaatggta taatttggta agaaaacact ttgactcata
    12481 ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat cgatctgact
    12541 gcggctgtag atggagtaga ggtgaaccta agacaggata agtgatgaat aaggtggcat
    12601 gaacaccttg aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa acattgaaaa
    12661 cgacaataat gaatttttgt tcc
//
LOCUS       DAYYHS010000080        12523 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000080 DAYYHS010000000
VERSION     DAYYHS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.080"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..329
                     /locus_tag="ACHA6A_002262"
     CDS             105..329
                     /locus_tag="ACHA6A_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM8970428.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            353..1114
                     /locus_tag="ACHA6A_002263"
     CDS             353..1114
                     /locus_tag="ACHA6A_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8970429.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            1126..1383
                     /locus_tag="ACHA6A_002264"
     CDS             1126..1383
                     /locus_tag="ACHA6A_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970430.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            1475..1726
                     /locus_tag="ACHA6A_002265"
     CDS             1475..1726
                     /locus_tag="ACHA6A_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM8970431.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            1702..1875
                     /locus_tag="ACHA6A_002266"
     CDS             1702..1875
                     /locus_tag="ACHA6A_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970432.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(1989..2396)
                     /locus_tag="ACHA6A_002267"
     CDS             complement(1989..2396)
                     /locus_tag="ACHA6A_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970433.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            2557..2781
                     /locus_tag="ACHA6A_002268"
     CDS             2557..2781
                     /locus_tag="ACHA6A_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970434.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            2791..2985
                     /locus_tag="ACHA6A_002269"
     CDS             2791..2985
                     /locus_tag="ACHA6A_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970435.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            2988..4010
                     /locus_tag="ACHA6A_002270"
     CDS             2988..4010
                     /locus_tag="ACHA6A_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8970436.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            3973..4788
                     /locus_tag="ACHA6A_002271"
     CDS             3973..4788
                     /locus_tag="ACHA6A_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM8970437.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            4806..5588
                     /locus_tag="ACHA6A_002272"
     CDS             4806..5588
                     /locus_tag="ACHA6A_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970438.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            5588..6436
                     /locus_tag="ACHA6A_002273"
     CDS             5588..6436
                     /locus_tag="ACHA6A_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8970439.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            6433..6675
                     /locus_tag="ACHA6A_002274"
     CDS             6433..6675
                     /locus_tag="ACHA6A_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970440.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            6818..7186
                     /locus_tag="ACHA6A_002275"
     CDS             6818..7186
                     /locus_tag="ACHA6A_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8970441.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            7201..7362
                     /locus_tag="ACHA6A_002276"
     CDS             7201..7362
                     /locus_tag="ACHA6A_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8970442.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            7359..7685
                     /locus_tag="ACHA6A_002277"
     CDS             7359..7685
                     /locus_tag="ACHA6A_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970443.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            7682..8497
                     /locus_tag="ACHA6A_002278"
     CDS             7682..8497
                     /locus_tag="ACHA6A_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM8970444.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            8499..8624
                     /locus_tag="ACHA6A_002279"
     CDS             8499..8624
                     /locus_tag="ACHA6A_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970445.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            8636..8947
                     /locus_tag="ACHA6A_002280"
     CDS             8636..8947
                     /locus_tag="ACHA6A_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8970446.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            8944..9189
                     /locus_tag="ACHA6A_002281"
     CDS             8944..9189
                     /locus_tag="ACHA6A_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970447.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            9186..9602
                     /locus_tag="ACHA6A_002282"
     CDS             9186..9602
                     /locus_tag="ACHA6A_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8970448.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            9599..9937
                     /locus_tag="ACHA6A_002283"
     CDS             9599..9937
                     /locus_tag="ACHA6A_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970449.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            9934..10164
                     /locus_tag="ACHA6A_002284"
     CDS             9934..10164
                     /locus_tag="ACHA6A_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970450.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            10171..10374
                     /locus_tag="ACHA6A_002285"
     CDS             10171..10374
                     /locus_tag="ACHA6A_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8970451.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            10371..10667
                     /locus_tag="ACHA6A_002286"
     CDS             10371..10667
                     /locus_tag="ACHA6A_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970452.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            10744..11157
                     /locus_tag="ACHA6A_002287"
     CDS             10744..11157
                     /locus_tag="ACHA6A_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8970453.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            11170..11556
                     /locus_tag="ACHA6A_002288"
     CDS             11170..11556
                     /locus_tag="ACHA6A_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970454.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            12107..12334
                     /locus_tag="ACHA6A_002289"
     CDS             12107..12334
                     /locus_tag="ACHA6A_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970455.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            12331..>12523
                     /locus_tag="ACHA6A_002290"
     CDS             12331..>12523
                     /locus_tag="ACHA6A_002290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970456.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         4553 a         1940 c         2801 g         3229 t
ORIGIN      
        1 aaaagttaaa ttaatttgaa cttttatgtt gacaatgttt tatgcaggag ttatactttg
       61 gttacaaagt tcaaaacatt tgaactagaa aggagcgaac taatatgagt tttgattatt
      121 ctaagttgtc gggaagaatt gttgagaaat atggaacgca atacaatttt tcaatcgctt
      181 taggtttgtc ggaaagaagt ttgtcattaa agttgaataa ccgagtacca tggaaaagca
      241 ccgaacttca aaaagccatt gaattgctag atattccaaa agaagaaatt ggcgaatatt
      301 tttttaattt agaagttcaa aatgtctgaa cttaagaaag gaattcgaaa aaatgaacac
      361 acaaaaattt gaaaacaatc tatttgaatt agaagttaag acggaaaatg gtgaatcgtt
      421 atttaatgct gaaacagtag caagaagttt gggtttcgtg gaaacaaaaa acggtaaaga
      481 atatgttcgg tggagaactg tcaatggata tttaaaaaaa tacctttcgc aagaagttgc
      541 gaaaaacgac tttatcagtg agccaatggt ttacaaatta gcctttaaag caaacaacgt
      601 acttgctgaa aaattccaag actggttagc aacggaagtc ctacctcaaa tccgaaaaca
      661 cggcatgtac gcaacagatg aactacttaa caatccagac ttgctgattg aagtagcaac
      721 caaactcaaa gaagaacgca cgttacgatt gatagctgaa caacgagtaa atgaattaca
      781 accgaaggca gactattacg accgaatctt gaacaacaaa ggattagtta cagttagtac
      841 gattgctaag aactacggta tgagcgctgt atcgtttaat aaattgctac atgaattagg
      901 tatccagttc aatcaaagtg gcacatggct gctatacagt aagttccaag acaaaggata
      961 cacgcatatt gagccatttg attatgaaga taaaaacggc aatcggcaag taaaaacaag
     1021 gatgaaatgg acgcaaaaag gacacatctt cttatatgaa acattgaaga agaataatta
     1081 cttaccgatg attgaacgtg aacaaacagc ataggaggta acactatgaa cgaggaaatg
     1141 agccaaacaa tcaaggtaca aaaaatgata gatgatctaa cacatgcaat tgatagccaa
     1201 gccgataaaa tcatcaagga attgcaagga caaaaagttt gggatgcaaa aatgcttttc
     1261 aagactatca aatttgaaat gaatccaaca aaaagaaagc tcgccgatgt gttggaagaa
     1321 aagttggctt ccgctatcaa cgagcaagaa ttactatttg aaaccgatac ttttaacagc
     1381 tgatgtttta tagatgacag tttcatccga atctatatca ttatagattc gattattgca
     1441 atgtacgaat tataaataca ggaggaaata accaatggag caactagctt tagttaacct
     1501 ctcagacctt aaagcttctt tggcagaatc tgagattgca aatgaagtgt gggatacaaa
     1561 gcaagctgcg gaatatttaa aaaccacaac acgcacgctg actaaagatg ccgaatctgg
     1621 aaagattcca gcagcgaaag ttggcagaga gtggaggttt agtagtatcg ctttgtatca
     1681 gtatctaaaa ggaggaaaaa catgggtaaa ttcaacagag cactagtgtt cagcgcaccc
     1741 ctaatcgtct atgctttagg actttgggga agcagacaag cattaatcgg cacgatcgtt
     1801 tacatggttt ggatttttat ggggcttgat gaagctgagt acagagcgaa aaagcccacc
     1861 gagggggctg actaaagtgt ggttctgttt attaggtgtt tatctcgtgg ccgttttagt
     1921 aaataactac atgagaaaac gtggcgaata ctggtataca tcctatgcgg ttctagtatg
     1981 tctaatgctt acaatttttc taatgattta ttcaaaatag gtaatacttt gtcaatcaaa
     2041 taagtttcaa gagatttttg ttctgcatca gtgttgccgt aaccaaaacg gcttaagaaa
     2101 attttcataa cctctatatc ttcattttga atatatggca ggactgcata acctgatgct
     2161 tttacagcag atatattttt atcagtgtta ctaccaatac aaatacccac attgttgaga
     2221 aatgttgaga aagttgtttg tattgtttgg gtttttgaat cgtgtttttg taattcaatt
     2281 tctttatcct tcatcgattc aacatgctta ttatttataa ccgctgtaat cgaaggggat
     2341 ataagagcta ctaaagctag gataatcgaa attgtgatcg tgttatcgaa actcattttt
     2401 tcaccatcca gtttttaaac cattatatca aaaaggagag aagaaataat gcaagaatta
     2461 gtaattttga aaaataaaaa aagacacgaa cgccagcaag catgatcgtg tccaaaagaa
     2521 aaatctttta aaccattata acacaggagg aaataaatga tctcagttaa agggttaggc
     2581 gatgaaatat tcgaagcaat gatgagcaaa gcacaacagg acatcaaaga aaaaatcatt
     2641 tctgctgcag cgcaaggaca aactagctgc actgttcgat cgagaggaac gacaccaagt
     2701 tttcttttgg cgctagaaga tgaaggcatt ttgaatatca aacgaagaga tggcagcgtc
     2761 agattgtttt ggcaatttta ggaggccgtg atgaaagatt ttgattcttt aggcgccaga
     2821 cagcaaccac caaatgaagc tagtcctgtt ggagttgatt ggcaagaaaa tccgttatat
     2881 ccaggtgata cttgctatct aacagaagaa ggctatgtac cagtagacgc aattctagag
     2941 tacgtccagc aacattatcc aaagattgaa ttaggaggaa tttaggaatg gcaaatgatt
     3001 taacacaaac aacacaacgt tcattagatg agcaggtaat cagtaatctt ggacggttac
     3061 aagaacaagg tttagaaatg ccaccaggat atagtccaca aaatgcgtta aaaagtgctt
     3121 ttttcgaact cactaacaat acaggaggaa acttgttgca gatggctgca aacaatcaag
     3181 aaatgaaaac ctccatttct aatgcacttt tagacatggt tattcaaggg ttatctccag
     3241 caaaaaaaca atgctatttc attaaatacg gaaacaaggt tcagctaatg cgttcatatt
     3301 ttggaacaat ggcggtcctt gatcgtgtaa ctggaggggc agatattacg cctgtagtcg
     3361 ttcgacaagg tgatgagttt gaagttgcaa tggatggacc aaacatggtt gtcaaaaagc
     3421 atgaaacaaa attcgagaat ttagacaatg aaatcattgc agcctatgta gtcatcaagt
     3481 taacaaatgg taaagagacc acaacagtca tgacgaaaaa gcaaatcgat caaagttggg
     3541 ctaagtcaaa aatgaaagat tctggaccgc aaaaggaatt tccagaagaa atggctaaac
     3601 ggacagtaat caatcgggca gctaaaacat tgatcaacac aagcaacgac aacgatttat
     3661 tagttcaagc tgcaaaagat acgttagaaa atgaatttga caatgatcgg aaagatataa
     3721 cgccacaaac agaaaaagtg gctactctcg aacaaaaatt cttttcaaac aaaaagatta
     3781 ctgaaccaat acaaaaagaa ccagatccga ttgtgattcc agatgatatc caaaatgaag
     3841 taactcgagt tgctgatgta cctgcccatc cagaaataga gcaagcacat ttgatcgaaa
     3901 atgaggatat caatccaatt caagaagaat tgatagatat tccagacttt ggacgcgagg
     3961 aaggtgtaga agatgtctca gaatttgaag acgacgagta ccctttctga tgagaattat
     4021 tactccaatg aagctgactg gcagtacatg tcaacatcac aatataagtc ctttttaaaa
     4081 tgtgaagccg cagccttagc aaagttgaaa ggcgattggg caccgacgtc ggatcccaaa
     4141 gccttactcg taggaaacta cgtacattct tattttgaat caaaagaagt acatgaagcg
     4201 ttcaaagaag aaaataaatc caaaatgttt tctagtagga agccgtatgg attgttgaaa
     4261 gatttccaaa ttgctgaaca aatgattgaa cgacttaagc aagaagaagc attcatgaac
     4321 atttatcaag gtgagaaaga aacgatcgtg actggggaac tatttggaac gacatggaaa
     4381 ggcaagattg attgcttgaa tgttgaggat gagtattttg ttgatatcaa aacaaccaaa
     4441 gacatgcatg agcggaaatg gaacgaaaac tacggatcaa gagaaacgtt cattgtcaat
     4501 tttggttacg tacttcaaat ggcaatctat caagagcttc ttttacagca atacggaaag
     4561 atgttcatgc caatcatcgc tgcggtatcg aagcaaacgc ctagtgaggc gaagttgata
     4621 acgattgatc aagataagat gagctacgag ctaatcatgc taaaagaaaa tattgaaaga
     4681 attgtcaaag tgaaaaatgg cgaagaaagg ccgatacctt gcggtacgtg cgagtattgt
     4741 cgaggtaacc tttcaattac tggattcact agcatggacg atctttaaaa ggaggtgctt
     4801 tgcgaatgaa tctaggatac atcaagttat accgtaaagt gactagttca ttcgtttgga
     4861 ccaattccga tatgttcaag ctatggatat tgtgtttaat gaaggctagt catgaagata
     4921 gaaaattttt atttaacggt caagaagtac gtttgacaag cggacaattc gtcacaggag
     4981 cccatgcaat agcaaaagag tacaacgaag gagtcccgag tgacaaagca attgcatggc
     5041 gaacgctatg gagatggctt aagaaatttg aaaatgaaga attattgaca atccaatcga
     5101 acgctagata tagcgttata acaataataa attggtctga ctatcaatcg agtgacaagc
     5161 cagtgacaag cgacggacaa tcgagtgaca agccagtgac aacaaacaag aatgataaga
     5221 atgataagaa tgataagaat aataatcctc gcaactctcg aaaaacacga gagtatgcag
     5281 atgacgatcc aaataaaaaa ttggccattc ttttattaaa actcattcga aagaatcgta
     5341 gtctcaaaga acccgatttg aacaaatggg ccaatacgat tcgtttgacg attgaagctg
     5401 acaaacgaac tggaaaagaa gttcaagaca tgattgtttg ggctactaac catgattttt
     5461 ggtccacagt catttcatcg ccaactagct taagaaaaaa tttcgataca atggtgggtc
     5521 aaaaaaataa aagaaagcaa caaaatattt ctaatgatga gttaccagaa acaggtgagg
     5581 attggtaatg aataaaaaac taagtgcaat ggctgcacct tacggcggat taaaaacagc
     5641 agatcataat tgcccgaaat gtggtgatcc attgtatatc tggaaaacaa aaaataaaga
     5701 tggtactgat cgatgcggtc ctacatgtat caataagatt tgtggttatc gagaaatggt
     5761 aactaaaaat caaaaagaag ctatccaaaa agcgaatgaa gcaatgaaaa aggacgctat
     5821 caatcgaatg cttaacagtt caatgattac agatgatgcc atatggacct tcgattttga
     5881 tggatacaaa gtagttgatc aggaaacagc acaaataaaa gcgatggctc aagaatgggc
     5941 taaaaaaatt gtaaatggta gcacgattca tgcagtgata accggtagaa caggagccgg
     6001 caaaacccat ctaggcgctg cagtgattaa agaagtcatg agggcatcta attataaaat
     6061 tgcttgttca tttatcagtt atcgagaact tttagaacag ttgaaatttg caatgaatga
     6121 tccagaagca agaaaagccg taacaggttc gctgatggct gagataaaga aatcagattt
     6181 cgtggtaatc gacgatttgg gtgcagaact aggaagaatg gaagaaaaca accaggcaac
     6241 gccttatgat gttgatgttc tcacatcgct cacagaagct cgtctaaaca aagctacgat
     6301 attcacaacc aatttatcat cgaagcaatt aaaacacgcg tacggtgagc gagtgttctc
     6361 tcgtgtaatg aatggaacga aagggaacat agctgtattc aaaacaacga cagacaaaag
     6421 gaggaatcca gtttgacctt tgtagtgaaa aaaatgtgct acttagatag ccgagggcga
     6481 ggagaagcta gtgtcgagtt tgctaaacac cacacgacta aagaagaggc tgaccttgtt
     6541 gcaagtgttt gtggcggcga agtagtcgaa gttattaaac ccaaaagacg attttcaaaa
     6601 cctaagacaa agccagtgaa aaaagagtgt tgttgtccga aaagtaacca agcatggatg
     6661 agaggtgcaa aatgacttgt ttaaaatgca acgatgaaac agttatttgg tataagacat
     6721 tgcttggatg gtcaacttgt gaaccttgtc caatatgcaa tgaaaatgga cgacgttcga
     6781 aagaacgact cgaaagacta aagaaggagt atagcaaatg gcaacaagaa gcaaatacgg
     6841 aaacaaaaag cacgaagtag acggcatcac ttttgattct atcacggaag cagagtatta
     6901 cgatctagcc ttgtggcaag cacaggcgaa tggttggaaa gtgaagctcc aggaaaggtt
     6961 tgagctgatg ccgaagtttg aactagaggg caagaagtat cgcaagatcg agtatattcc
     7021 cgacttcaca ttttacaaaa gcggcaagct ggtcaaagtc gtagatgtta aggggatgca
     7081 gacaaaagac tttaagatca aggcaaagtt gttctgtcat caatatcaag tgccgttgat
     7141 attagccaaa aaatatcgga atacgttcaa ggaagagcgt ttttaacgag gtggtccatc
     7201 atgacaacag aagaagtgat tcaaatgcga attcgaagcc ttcggcgtga gattgacgat
     7261 ctggaacgaa caaaggcagt gatggtcaat gaaacggcta gaaaggcaat cgatttgcac
     7321 atagagaatt taagaaggga aatccgtaga ttggaggaat gagcgtggat aaggaagcgg
     7381 cttggcgaaa attaatgttg ctgattcaag atgagaactg gcaagaagat gaagcagtgg
     7441 ttgctgaagt tcagcgtcta ggaaagattg ctaacggacg tatacgaaaa aagccagaca
     7501 aaagaaaaca gcgcaaaggg aaaatcgtcg ttgttttaca cgaaggcaaa attttgatgc
     7561 aaggaacagc tagtgagctg tctgcagaaa ctggatatac gcgtgggact attcgaacgt
     7621 acgcttggcg aaatcacacc gatcgaaaag ggcacgaata taagtatttg gaggaagaaa
     7681 aatgaacgaa aacaaattaa tcaaattggg tgtagcagga gcagtaatcg taggtattgg
     7741 agttatcgga ggatttaagt tcttcgaaaa aatcgataat ggatatgtgg gtgtgcgcta
     7801 ttcaatgaac ggcggtatca aagatgaagc actgacgcaa ggtgtgaaat ttgtagggat
     7861 tgacaaagtg atccaatatc caattcgctt gcaaactatc caatcaaaaa atatttcagt
     7921 atctacaagc gacggcaaaa agacaacgat tgatatcaaa tatgactaca aagttgattc
     7981 aactaaagca gcaaaaatgt acaaggaatt tgggaatatc acttcggaag atatcgaaag
     8041 tggatggtta aaatctaagc ttcaaaaggt cgctcgtgaa gtttatgcga aatatagtct
     8101 gcttgatgtc ctttcaggag attcctctaa agttgaagct gaggtattaa cgaactttgc
     8161 taaatcagtt gaatctaaag ggtttgaagt cgaagacgta acacttggtg ttccagatgt
     8221 cgataaagaa acacaaaaat caatcgatgc gatcattcga gctggtcaag aaaatgaaaa
     8281 agcgaagcta gacgcagaaa ctgcaaaaac tcaagctgat agtgaagctt acaagaaaac
     8341 aaaagctgca gaagcagagg cagaatctaa tcgcaaagtc gccgaatcag taacagacaa
     8401 tttgattcgt tatgaagaag ctcaagctcg caaaaagcat ggatgggtaa cagtaaatgg
     8461 agcagatact gtagttacgg atgaagcagg caaataatat gggattcttt atggctaaaa
     8521 ttctcttgtt cttaggttta gtaggagcag catatctcgt gtatgcgctc ctttcccaaa
     8581 ctgatgacaa agaagatgac aacaacgatg aaatgaaatt ttaggaggag aaataatgga
     8641 cgaactaatc acaaaagtag agcagtgggc taaagacaag ggactggatc aagcagatcc
     8701 aaaagcacag tttttgaaag tagctgagga attcggggaa atagcttcgg cgatggcaag
     8761 aagtaatgat gaactattta aagatagcgt aggagacgtt atcgtcacgc tgattatcct
     8821 ttccatgcaa aaagggacaa acgtacaaga gtgtttagaa atggcataca acgaaatcaa
     8881 aggacgcaca gggaaaatgg tagatggtgt attcgtgaag tcgagtgatt tggagggcag
     8941 caaatgataa aaaagctcat tcaattcagc atggatttat atgatatcga atcaggagca
     9001 acactatctg tggaatcgga ccatctaatc ataaattttg gtggaaagcg ccagattatt
     9061 ttgtgggtag ttgatgatgt actgtttcca gaaattgttc atgatttcga agaatcaaaa
     9121 gcggttgagt ttgaaatagt gaaaaaagta atggaattga ttgaaaaata cgaggaggac
     9181 agcgaatgat accgaagttt agagcgtggg ataaacgaaa gaacgtaatg agagatgtag
     9241 ccgtcttgca ttttactaaa aacggcaaaa caaactttat tgaatattgg ataaatccta
     9301 ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat gcaatccaca ggaatgaaag
     9361 ataagaatgg tgtggatata tttgaagggg atatagtatt agtcagcgtg cgaaatggct
     9421 tcgattactt agataataaa gtttgtattg tcaaaaattc aatagattat tccggattag
     9481 tttgtgccac tgttgatgaa gacttagagt atcaaatttt taacacagag ctgtttgaag
     9541 aatacacgta tgaagtcatc ggaaatatat acgagaatag cgagttattg gaggtagagt
     9601 gattatctta gctactgact acatcgattc tttaaaagat gaaaatggga atgtcccagc
     9661 attgctaaat tgtggaatta aaagcaagaa tagaatcatt ttgttacgga tatcggctga
     9721 tttagcaaaa tatctttatc tgagcaagac agcagtatat tacactgcgg ctacacgtta
     9781 tcagtacaag ggaaaaacaa tcagtcagga ttattatgat cgtttaatca gtcctgatat
     9841 gtatgggaat tcagaatcag caattaagct gtttggcgca atagagatat atgccgatga
     9901 ttttccaaat ttatggttga aggaggaagc ggaatgaaat acgaaatacc actaagtgaa
     9961 gcagacgtcc agtcaattat taacggtcgg gaggttaata aaaaacttcc tgatggtact
    10021 gaattagtca tcagacaaag ttatttgaaa gatatggcag ctccagtatt aattgatcgt
    10081 tttaacgtga ctgattctgt ggtagagaac cacttgaaag aatttcgatc aagtataaac
    10141 gacactttca gattagggag ttgattgaca atgaacacca gacaccgcag agtagcaaaa
    10201 ctaagaaaac aggaactgaa tgtactaaag acaaagtttg aaaaagaata tggaatttca
    10261 gcagaagaaa catataaagt ggcaagtcag tgtgttgctg atgcaagtga tgctattcgt
    10321 aagtttggaa tttcgatatt aagtgatgat cgtaaatggg aggaaaaaga atgaaactaa
    10381 aagacggatt ttacgctagc agtcacggta tcggcggttt aatgctagat atgccgacaa
    10441 agaatcctaa aacacgtaag aaaccaaaag tcaaagtcgg tgacatggtc cgctgtgaag
    10501 cagaggagtt catttatcca tttcgtggat atgtagaaca cgtctataat cactcagcga
    10561 tcattcgcat tgaaaacacg atggaatgtg ataaatggac agctaaaagc aaagagaatt
    10621 tagcagtagt gagattggtg gatatggaac taatcaatga caaataaaaa agccggatcg
    10681 ctccgactaa cataataaaa cagacaagtt tattatatca cataaaagga gcagtttgaa
    10741 ttgatgcaat tgttacgaga ggtagatttc aaacagacaa gatgtaatgc gagagatgtg
    10801 ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtcg
    10861 ccgattatta cggatatgcc gaaggcaccg aagcacggca ataaggcaga ggatgcattg
    10921 attcagatga tggatataga agcggagaga gacgcgattt tagcggcttt gatggctctt
    10981 agtctgatta gtcgtcagat actctactac agtttttgtg tgccagacag cttctcaaac
    11041 tacagaatta gccgtgaagt gggttattca gaaagaagta tacaacggat gaaatcggaa
    11101 gctctaatag agtttgcaga agcatataaa cacggaagaa taattgctta taaatagggg
    11161 gaccatataa tgtggaaaga ttatgtatcg ttgaaagagt tgaaaaaaga tcttgttttc
    11221 aaaagaattg tagaatggtc agagagtgaa ttaattttag aagatggaac caaaatggaa
    11281 gttgtatgta gcgaatcaga ttgctgcgca tgggccgaag gtgaattcaa aaatgtaaag
    11341 cttgatgcag tgattacaga tataaagatt tttgataaag gtaaccgtct ctataatggt
    11401 gacggacata cctcttatgc tgaagtcgtt gtttatcata atcgtaatga gatcgctaaa
    11461 gcagaatgca cagcgaatga tgggaatggc ggctactatt atagtgtttg tgctttaaag
    11521 gtcaaagaca aactttgtat agtgactgac gcataaaaaa atggcggttt tttggcggaa
    11581 tgatggcggt ttttagccat ttatcagtga tattatggta gtgtcgaaag attagtgata
    11641 ggtctgagac aaaataataa taaaaggaac atcgttttat tattgtttca caattaagct
    11701 tcgatagaca gcaacggaaa tattaagaat aaggatgtga attttaactc cttctaaatt
    11761 gttcttatta tctatcatcc gttgctgttt attgttacat acagatcgct taggcggtct
    11821 ttttattttg gccgaaaacc tacattttcg atggccgatt attggaggag gaatagcatg
    11881 ttcaaactat ctgaaatcat taagaagtcc gacgttgaga aattagagat gttaaaaaag
    11941 aagttgaaga aatagcagtc tcttcgtgag gctgttttat tttgctcaca aaaatagacc
    12001 actatcgggt aatagtggtc aggaattaaa tgaaaaagat gttagggttg ttagctaagt
    12061 ataacatcat aacgcttaca aaacaatgca agaaggataa attattatga gaacttactg
    12121 gtatgtgtca ttaaacaata aatatccgaa gccaatgaaa ggacagcata ggcgtgtagt
    12181 catgtctgtt caaatgaagg cgaagtattc gattgtagaa atgctaagag aagctacacc
    12241 aaaagagatt gatcagtata agttggttct tattggcatt ggttggttta aggatgaaca
    12301 tattcaaaca aacatgaaga ggtggttgag atgaagttat attatgttga aaaagcaaat
    12361 tgttgggtgg ttgaaggctg tctgtcaaag tacgctacag atattatatc tactcaattt
    12421 tcagagttgg gattgaaggt tatcttttgt ggtgagtttg tcagtgtgaa agaagcggta
    12481 ttgccaatga cagcaaagag cagacgctcg cgtggttcaa gtt
//
LOCUS       DAYYHS010000081        12136 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000081 DAYYHS010000000
VERSION     DAYYHS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.081"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHA6A_002291"
     CDS             <1..610
                     /locus_tag="ACHA6A_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8970457.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHA6A_002292"
     CDS             723..1001
                     /locus_tag="ACHA6A_002292"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8970458.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHA6A_002293"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHA6A_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8970459.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHA6A_002294"
     CDS             1458..2213
                     /locus_tag="ACHA6A_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8970460.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHA6A_002295"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHA6A_002295"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8970461.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHA6A_002296"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHA6A_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8970462.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHA6A_002297"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHA6A_002297"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8970463.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHA6A_002298"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHA6A_002298"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8970464.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHA6A_002299"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHA6A_002299"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8970465.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHA6A_002300"
     CDS             6950..7492
                     /locus_tag="ACHA6A_002300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8970466.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHA6A_002301"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHA6A_002301"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8970467.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHA6A_002302"
     CDS             9085..9987
                     /locus_tag="ACHA6A_002302"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8970468.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHA6A_002303"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHA6A_002303"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8970469.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHA6A_002304"
     CDS             11430..11852
                     /locus_tag="ACHA6A_002304"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8970470.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAYYHS010000082        12013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000082 DAYYHS010000000
VERSION     DAYYHS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.082"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..654)
                     /locus_tag="ACHA6A_002305"
     CDS             complement(67..654)
                     /locus_tag="ACHA6A_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970471.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            829..2151
                     /locus_tag="ACHA6A_002306"
     CDS             829..2151
                     /locus_tag="ACHA6A_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8970472.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2306..3082
                     /locus_tag="ACHA6A_002307"
     CDS             2306..3082
                     /locus_tag="ACHA6A_002307"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8970473.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3075..3644
                     /gene="rnmV"
                     /locus_tag="ACHA6A_002308"
     CDS             3075..3644
                     /gene="rnmV"
                     /locus_tag="ACHA6A_002308"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8970474.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3637..4521
                     /gene="rsmA"
                     /locus_tag="ACHA6A_002309"
     CDS             3637..4521
                     /gene="rsmA"
                     /locus_tag="ACHA6A_002309"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8970475.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4972..5601
                     /locus_tag="ACHA6A_002310"
     CDS             4972..5601
                     /locus_tag="ACHA6A_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970476.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5725..6834)
                     /locus_tag="ACHA6A_002311"
     CDS             complement(5725..6834)
                     /locus_tag="ACHA6A_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970477.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7043..7465
                     /gene="mgsA"
                     /locus_tag="ACHA6A_002312"
     CDS             7043..7465
                     /gene="mgsA"
                     /locus_tag="ACHA6A_002312"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8970478.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      7652..7750
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            7788..8324
                     /locus_tag="ACHA6A_002313"
     CDS             7788..8324
                     /locus_tag="ACHA6A_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8970479.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8364..10127)
                     /locus_tag="ACHA6A_002314"
     CDS             complement(8364..10127)
                     /locus_tag="ACHA6A_002314"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970480.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10130..11845)
                     /locus_tag="ACHA6A_002315"
     CDS             complement(10130..11845)
                     /locus_tag="ACHA6A_002315"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970481.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3755 a         2218 c         2449 g         3591 t
ORIGIN      
        1 atagataaaa aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa
       61 atcctatcac ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag
      121 agcagtaagc agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc
      181 ataatgttga tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga
      241 gccagctata aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa
      301 aggatagaaa taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc
      361 gatattcaat aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac
      421 caaaaaggaa tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat
      481 tgcgatgaac aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc
      541 aaataaaatt aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc
      601 gacccggttg gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg
      661 ttcctccttc gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt
      721 cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc
      781 agttatactg aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa
      841 ctaaaacgag aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt
      901 gcaggcgtct tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca
      961 ttgctagctt ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa
     1021 ttagctacag caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga
     1081 aaattgccca gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt
     1141 gctgcattat cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat
     1201 tatcttttgt tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg
     1261 acaaaagtcg gtgcttctgt acaatcagta acgttgattg ttaaattaat accaattgct
     1321 gtgattgtca tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc
     1381 gaagctggaa aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg
     1441 tttgcttatg acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa
     1501 aaagatttgc ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg
     1561 atcaattttg tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca
     1621 gcttctgaag catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg
     1681 atcttgatct cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct
     1741 tatgcgatgg ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag
     1801 aagtttacgg ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg
     1861 agccttggtt ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt
     1921 ttactgattt ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca
     1981 tataaagtac cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt
     2041 ttaggtatga cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg
     2101 ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca
     2161 acaacagaca gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa
     2221 taaaacaagt tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa
     2281 gtaagaatga aaaggagaga taaacatgat atttgattct catacccatt taaatgctga
     2341 acagttcaat gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa
     2401 aatggcggtc gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga
     2461 ctatccaaat atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa
     2521 agaaatcgaa aataaattac aagaacaatt gactttacca aaggttgtag cattagggga
     2581 aattggcttg gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg
     2641 cagacagatt gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc
     2701 acttgaagac acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat
     2761 gcatagtttc agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat
     2821 ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa
     2881 tgtaccaatc gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt
     2941 tagaggaaag cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact
     3001 tcggaatctt ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt
     3061 tggtctaagc gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag
     3121 acgatacaag aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg
     3181 caatcaatga agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg
     3241 tttttaccga tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc
     3301 ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc
     3361 ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc
     3421 cagacatttc ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt
     3481 taatagctgg ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg
     3541 gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag
     3601 aactcgccca agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg
     3661 acaccctcta gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta
     3721 ggacaaaatt ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata
     3781 gatgaacata cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta
     3841 gccaagcatg caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg
     3901 gctgatacga tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc
     3961 gatttgtcaa cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg
     4021 aatttacctt actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt
     4081 gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca
     4141 ggaacgaaag cttatggttc attatcgatt gctgttcaat attatatgga agccagttta
     4201 gcattcatcg taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg
     4261 aaactgacaa gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga
     4321 ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg
     4381 tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc
     4441 gatccaaaac gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg
     4501 atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct
     4561 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg
     4621 cttattaccg cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg
     4681 tttaactcta agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt
     4741 tggtcttatt acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca
     4801 caaattttgt cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact
     4861 cacactcatt ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga
     4921 gttatactaa gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa
     4981 aattcagttc gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca
     5041 atggtcatgc ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca
     5101 ttatttattg cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca
     5161 tttggtttct ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg
     5221 tttgcgttga ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga
     5281 cagtttactt ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat
     5341 tctttagcag aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca
     5401 tattattctc aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc
     5461 cacagtttag ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt
     5521 gatatccccg tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac
     5581 gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg
     5641 ataataaatc ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct
     5701 ggcacagctc taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc
     5761 aaagaagtgg cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa
     5821 atctcttgca tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt
     5881 ttcagctcca agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc
     5941 ttcaagggct acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt
     6001 tccttcataa cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag
     6061 cccatgtcca tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg
     6121 agatccaata aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt
     6181 accgatttgt tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc
     6241 ttctgtctga tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc
     6301 aatctcagca cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat
     6361 taagaagact tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc
     6421 accagataaa gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc
     6481 attgtccaca cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc
     6541 catattatca aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat
     6601 atcacggtct ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc
     6661 agagatatct tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg
     6721 accaacaaaa acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata
     6781 attttctgca ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg
     6841 ttccacactt tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta
     6901 acttcaatgt catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag
     6961 ctttaataaa tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac
     7021 aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc
     7081 tatgatggtg aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc
     7141 taccgggacg actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa
     7201 atccggaccg ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga
     7261 tttggtgatt tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc
     7321 acttatccgt ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga
     7381 ggtgctacta agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat
     7441 agacaagcat ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc
     7501 gcctatatgt agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat
     7561 tggataatat aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta
     7621 tagtttctgt gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc
     7681 taagaggatg ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc
     7741 attcgctgca tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc
     7801 ggttagacgc acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt
     7861 cccgactggt agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag
     7921 tgatcatggg cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt
     7981 tagtaggaat ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat
     8041 ttattgaagg agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg
     8101 ccattctggc tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc
     8161 ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa
     8221 aaacagtaat atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc
     8281 cttacaaaaa aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc
     8341 ccatcccaga agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg
     8401 taataaccat ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga
     8461 tccatcacta atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa
     8521 ctggtttttc cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta
     8581 tcgactgagc tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga
     8641 gcaatcgtca acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta
     8701 gtatctaatt tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca
     8761 tcattgattt ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta
     8821 ctgtcaaata gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt
     8881 tggatagagc ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa
     8941 cgcatcagca aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg
     9001 gtttgacctg gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa
     9061 gaaaaagaaa catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag
     9121 gcatctggca aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga
     9181 ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa
     9241 aactgacttg agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc
     9301 ttcaatgcca ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa
     9361 cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga
     9421 tcagcattta gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg
     9481 attttctggt taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc
     9541 tgtctatcga aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt
     9601 ggtgtagaac aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac
     9661 gaaagaatga tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg
     9721 gaaatattgt ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca
     9781 taataacgaa gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg
     9841 gataagtaag ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt
     9901 aaaacgatac caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct
     9961 tctccaatca tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc
    10021 ccattgatga ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa
    10081 ggaatgaatc ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct
    10141 cctgtgatgc gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac
    10201 cctgagcagc aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc
    10261 ggacacgttg ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca
    10321 aatccaagtc tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg
    10381 gcttggcaat caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt
    10441 tcccattttc catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg
    10501 cctgagcgat tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt
    10561 gaaggttttc acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt
    10621 gctgacggag atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac
    10681 cggaactgac atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg
    10741 ttggaccaat gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag
    10801 aaagaccaga atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag
    10861 aagaaaaatc gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca
    10921 cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa
    10981 caacgatcag tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt
    11041 gttgcaagct accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga
    11101 cattcatgat caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat
    11161 aattgttcgc tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg
    11221 caaatgcgcg gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat
    11281 ctagtttttc ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa
    11341 tgattgagaa aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa
    11401 aagccatgac cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca
    11461 ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg
    11521 ttccaaaatg attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac
    11581 gtaactctgt accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac
    11641 aaatcagacc aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc
    11701 ggtcattcat ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt
    11761 cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt
    11821 ttgcataacg taataactga atcatatgat tcctcctatt atttatttgt ccacaattga
    11881 aaaaacaagt actaccacat tgtagcttag gaaatttaga atgaaaagag aacaagcctt
    11941 ttcataaatg aaagttttat tatgaagaag ctttagaaat tgaatagact atttttttta
    12001 aaaaaaagaa aat
//
LOCUS       DAYYHS010000083        11908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000083 DAYYHS010000000
VERSION     DAYYHS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.083"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHA6A_002316"
     CDS             <1..465
                     /locus_tag="ACHA6A_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8970482.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="ACHA6A_002317"
     CDS             complement(1080..2120)
                     /locus_tag="ACHA6A_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8970483.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="ACHA6A_002318"
     CDS             complement(2469..2795)
                     /locus_tag="ACHA6A_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970484.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3570)
                     /locus_tag="ACHA6A_002319"
     CDS             complement(2782..3570)
                     /locus_tag="ACHA6A_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8970485.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="ACHA6A_002320"
     CDS             complement(3938..4228)
                     /locus_tag="ACHA6A_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8970486.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="ACHA6A_002321"
     CDS             complement(4364..4543)
                     /locus_tag="ACHA6A_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970487.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="ACHA6A_002322"
     CDS             complement(4540..4890)
                     /locus_tag="ACHA6A_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970488.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="ACHA6A_002323"
     CDS             complement(4883..6208)
                     /locus_tag="ACHA6A_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8970489.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="ACHA6A_002324"
     CDS             6658..7272
                     /locus_tag="ACHA6A_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8970490.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="ACHA6A_002325"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="ACHA6A_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="ACHA6A_002326"
     CDS             complement(7883..9202)
                     /locus_tag="ACHA6A_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8970491.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9336..9944)
                     /locus_tag="ACHA6A_002327"
     CDS             complement(9336..9944)
                     /locus_tag="ACHA6A_002327"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970492.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10582..10839)
                     /locus_tag="ACHA6A_002328"
     CDS             complement(10582..10839)
                     /locus_tag="ACHA6A_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8970493.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10832..11101)
                     /locus_tag="ACHA6A_002329"
     CDS             complement(10832..11101)
                     /locus_tag="ACHA6A_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8970494.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11232..11411)
                     /locus_tag="ACHA6A_002330"
     CDS             complement(11232..11411)
                     /locus_tag="ACHA6A_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970495.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11509..11745
                     /locus_tag="ACHA6A_002331"
     CDS             11509..11745
                     /locus_tag="ACHA6A_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970496.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3675 a         2151 c         1826 g         4256 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
    10021 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
    10081 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
    10141 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
    10201 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
    10261 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
    10321 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
    10381 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
    10441 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
    10501 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
    10561 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
    10621 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
    10681 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
    10741 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
    10801 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
    10861 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
    10921 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
    10981 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
    11041 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
    11101 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
    11161 aaatgattaa tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat
    11221 aaaaagtgac gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc
    11281 aataaatcta acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt
    11341 tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt
    11401 gcaattggca ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca
    11461 atcatcttga atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat
    11521 accagtattg ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt
    11581 atttcgccaa caaataggac taacaataga ggaatgattt tattcatatg gatctccttc
    11641 tttctcttag gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg
    11701 ttggagcgat tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat
    11761 ttcacacatt ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt
    11821 gatatggcat tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat
    11881 aggtgtctaa aaaatcagtt aaagtcgt
//
LOCUS       DAYYHS010000084        11781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000084 DAYYHS010000000
VERSION     DAYYHS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.084"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            507..1070
                     /locus_tag="ACHA6A_002332"
     CDS             507..1070
                     /locus_tag="ACHA6A_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970497.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1131..1496)
                     /locus_tag="ACHA6A_002333"
     CDS             complement(1131..1496)
                     /locus_tag="ACHA6A_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8970498.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1572..1817)
                     /locus_tag="ACHA6A_002334"
     CDS             complement(1572..1817)
                     /locus_tag="ACHA6A_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8970499.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1887..2141)
                     /locus_tag="ACHA6A_002335"
     CDS             complement(1887..2141)
                     /locus_tag="ACHA6A_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970500.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2305..2628)
                     /locus_tag="ACHA6A_002336"
     CDS             complement(2305..2628)
                     /locus_tag="ACHA6A_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970501.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2815..2888
                     /locus_tag="ACHA6A_002337"
     tRNA            2815..2888
                     /locus_tag="ACHA6A_002337"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2849..2851,aa:Ile,seq:cat)
     gene            complement(3273..3770)
                     /locus_tag="ACHA6A_002338"
     CDS             complement(3273..3770)
                     /locus_tag="ACHA6A_002338"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8970502.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3810..4619)
                     /locus_tag="ACHA6A_002339"
     CDS             complement(3810..4619)
                     /locus_tag="ACHA6A_002339"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8970503.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4953..5810
                     /locus_tag="ACHA6A_002340"
     CDS             4953..5810
                     /locus_tag="ACHA6A_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8970504.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5803..6705
                     /locus_tag="ACHA6A_002341"
     CDS             5803..6705
                     /locus_tag="ACHA6A_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8970505.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6738..7088
                     /locus_tag="ACHA6A_002342"
     CDS             6738..7088
                     /locus_tag="ACHA6A_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8970506.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7137..8060
                     /locus_tag="ACHA6A_002343"
     CDS             7137..8060
                     /locus_tag="ACHA6A_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8970507.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8138..9031
                     /locus_tag="ACHA6A_002344"
     CDS             8138..9031
                     /locus_tag="ACHA6A_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8970508.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9069..9632
                     /gene="amaP"
                     /locus_tag="ACHA6A_002345"
     CDS             9069..9632
                     /gene="amaP"
                     /locus_tag="ACHA6A_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8970509.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9646..9834
                     /locus_tag="ACHA6A_002346"
     CDS             9646..9834
                     /locus_tag="ACHA6A_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8970510.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9847..10749
                     /locus_tag="ACHA6A_002347"
     CDS             9847..10749
                     /locus_tag="ACHA6A_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8970511.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10781..11023
                     /locus_tag="ACHA6A_002348"
     CDS             10781..11023
                     /locus_tag="ACHA6A_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8970512.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11279..11650)
                     /locus_tag="ACHA6A_002349"
     CDS             complement(11279..11650)
                     /locus_tag="ACHA6A_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970513.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4175 a         1985 c         2233 g         3388 t
ORIGIN      
        1 cattggtcgt tgaatgggct gaatagtagt gtataaaaca attttgtgga gcgtgaagct
       61 tttatggtag aggaaaatac gaataaagtg atgttgataa aaagattcct atcttgaata
      121 tgaatcgaga tgtcttgaaa atgaatgagt ttgaccaatc attatcctat cgaaatttac
      181 agtttcgttc gattggtctg tcaattaatg aaaaagtaaa aaagaatatg ttctaggaag
      241 agggcggcac atctaaattt ttaagtaacg gacagttcaa atatcaagca ttgaaagagg
      301 ctatcctact gtttgaagga atacgaatat ataaaaacga caaagaggaa aaagtagttc
      361 attaaatccc ttcaagtgat gaaagaatga gatctaataa aacctgatct ttcatctgtt
      421 tttttaataa cgtaataatt tttttatttt ttgtgctttc gccgttattg tagagtgatt
      481 tagcattctt atttttaaaa ataactgtgt tatatattaa aaaaggcata atagttataa
      541 gcttttttaa agaatattta gaaattatta attttcgtct gaaaacaaaa aaagaaagcg
      601 ttattatttt agtctatttt caggggggga accttatgat aaaaaaacta tttttactat
      661 caatattaac gctagcaatg gcaggttgtt ctgactctgg cttagcaact gaactgatca
      721 caaaatcaac tgaagccacg acagaggagt taaaatgtgg aaacgatcac agtgactaca
      781 gaggcttaaa tcgacgatgc atgggaatat taggcagaag caattgtaag catcgatgta
      841 gtaatcgaag aaggtgtaga agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca
      901 atctgtgctg tgcatcgtat acagacgtcg aagatgcggt acttttaaac cacgtcagga
      961 gatgttcatt tatgtctagg gaagttggcg atgaaccaaa actagagtac gcttttagtt
     1021 ttgcacgtga ggatgaagat aaccctaaaa cttacgacat tttagcttga tctaagatgg
     1081 ttaaagcttt ttaatgaagt aagaggagat agaaggcttt tctagttata ttaggtaaga
     1141 taaattcctt tttgctccaa atttgctaaa cattctgcta gataaacttc atcgtgttcc
     1201 ggataaatcg gcacgttgcg ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg
     1261 ccacgataca taggattttt ccactctggt gctgggctgt aaccacgttt ttccatttca
     1321 tccatgacaa gctgatgata ttggaataat ctataaggag aatagtgaaa aacataatcc
     1381 acagtagcat gtttctttcc ccagccattt cctcttaatg cacagcattc ccgatgttgt
     1441 cctaaaagct gttgacgagg aagctttgaa atcaattttt catgccataa tcgcatagaa
     1501 atgtctcctt tatatataga ttgttaacct tagacgttaa atgaaaaaac cagcaccaag
     1561 gctggaaaaa atcacttcag tttgcttgct gtttctgagc gaccaattag gtaatcaacg
     1621 gaaatatcgt aaaagtcagc gatatagatt aattcaatga tgcttggaat ttcttctcct
     1681 tcttcgtact tacgatattt gaataaagga atacttaacg cgctagaaac agctacttga
     1741 gataaagatc ggccagttct taagaaattc agacgacaac gtaaagtatc cagtaaaata
     1801 gcttcttcga tactcattta atcacctttc attatagtag aatagttata atgactatag
     1861 aaagctacac atgcaatcaa tagtactcaa tgttttttat ttaaacgtat caaataaaga
     1921 atgacgatca ataaagacaa aaccagtcct gataattctt ctataaaaac actatcgggt
     1981 tcaaagtagt tgtaattaaa aactttccac ataaagtaga tagccggaag agtcaaaata
     2041 gctaaataaa accgttcttt gttccaattc ataagataaa caatacttgc gaccagtgcg
     2101 accgcaggca cccaaaacaa aactctcacc aacgtaatca tgtaacggac tcctcaacga
     2161 ttcatataat aaaagataac atgttattgc atacatcaca aatgaaatgc taggaaaata
     2221 aaagaagaat agatcaatga aaaatgaaaa agcagaacag ttttctgctg ataagcagga
     2281 actgttccgc tgaatgtgtc ttatctatcg agaaaaaatc aatggattga tatctaagta
     2341 attcaataca tataaaaaga ggacgatcgc attcactaaa aaggaaagaa tgatgatccc
     2401 atagcgaatg gaaacatatt taacaaatac ttttgtttga atacgggcat atctttttcc
     2461 tttatagctg agcatcatga caagaagcaa aagggtaatc acaccttgaa aaatcattcc
     2521 aataaacaga attcctgagg tttgataatt taattggaga aaataagctg ctgccgcaac
     2581 taaatcgaat aaaatcgcaa taaatggcaa aataatcact cctaacattg agaataaata
     2641 aaagaataac attttttcta aaataagcaa ataaaaatta aagtaacaaa atagttatga
     2701 cacaagtaaa agaaataaga ataacaatat tattttactt atattttgaa acttgtgaga
     2761 gtttgtcatt cgtgctatag ttgaaaagta acaaaaataa attttattta tgatgtacct
     2821 ttagctcagt tggttagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
     2881 aaggtacata agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta
     2941 aaaacagctt cttatgtttt tacttggctg ttcttattac tgcagatgca agcttttgaa
     3001 taccgtttaa ggaagaggtt gtgaaatcag cgttttgacc tgagtattag cttaacaaac
     3061 ggaaaaatag ttcctcatat tttgccgttt gttaagctaa taccgagggt cagctgattg
     3121 aacaccattt aagaaagagg tcgtgagcct gccgtttcac tctgtgtcat aaaaaagcca
     3181 atctactttg atagaaaaag cacgtaggac taaaacagtt ctgcgtgctt tttttattgt
     3241 gtgttaaata tcgtaaatga agatagatat ttttatgatt cgacgataga gacgttgaaa
     3301 tctttcaaca gctctaaagc ggattctccg tgttttctac tggagactaa cattggatca
     3361 acaaaaaagt ctgcttcagg aaaagcactc tgcaggccta tcagattaga gagtacacac
     3421 aagtttgttt caatcccgac tacttcgatc tctttttgtt cttctttgct ttcaaataaa
     3481 gtttcctgga tttcagctaa agtttcaggt ggaatcgtaa agtaatactt tttaatcact
     3541 cgttcatttt cttgtggaga aagaagaggg atcaattgca aatcttctcg ttcttcttct
     3601 ttgtccttca gttcaactgg tatatcccgt gtgaagagaa tatattcatt attttggcgt
     3661 gctttagcta atctttgagc aatacgttct gccaattgat ctgactctgg aatataaaat
     3721 tcgctgtttg gatcaagaat atgattttgc atatcaataa taactaacat tttctttcct
     3781 cctacattca tatcgtcatt ttattctttt taatagaagt tttttaaaat ataagcaaca
     3841 ccagcttctt cattcgtcag tgttacttgg tttgcaattt cttttacttg tcgctgtgca
     3901 ttccccatcg caatactcaa cccgctgtaa ttcaacatag caatgtcgtt ttcttgatcg
     3961 ccaaaagcca tgatgtcact cgattcgatt ccaaggtaat ccgccagaaa aacaagtgaa
     4021 tgagctttag tgatacctgt aggcgtgatt tctaagaaat gcggatcaga gaatactgat
     4081 tggataccat ctgcttgcag atctctttgt agcttctgta gtttttcttc actcttatct
     4141 gttaatgcta atttaagata gctaagagaa ggatcttcca acatatttaa aatttgatca
     4201 aaagaatggt tctgtaaatt gaaatgttca taataagggt catctttgac tagatttgat
     4261 tcatcgaatg tcagctgatc atcttgggtt tctcggctag caatgatgga tattttcgct
     4321 tcttgagcat agaagaaggc ttttctcagt gtatcgctat ctatttttga acgtctgaga
     4381 actttcgcca tttttccatc cttcatttca gcaatcagtg aaccattgga agaaatcaaa
     4441 tagccaccga ttcctaattc ggaaaaaata tctttagcag aaatgctgtt ccttccggta
     4501 gcgacggtta cgattccgcc attttcatca aatttccgaa gtgcttggat attttcttct
     4561 ggtatctttc catttcgatc aagcaaggta ccgtctaaat caatcgctgc tagtttcatt
     4621 ttatcttcct actttctttt tacatatcta ttttcctgtt accttaagat taaatgagaa
     4681 tttaaaaaaa tgaaactaac ttgctttgtc attatgaatt ttttcaaaaa acattcttaa
     4741 gaaaaaggtg gctatatata gtagtttttg gtcaatacct ttgaataaaa agccatgaaa
     4801 aacgaatagt tagcgcttac cgatgcgttt tgtgataaaa aaattttaaa atattcaatg
     4861 aaaacaaagc atatattttg ctttaaaacc aaatgaggaa tattctagat gtagaacagg
     4921 ttagaaaaag attagaaaaa ggagtgattt tgatggagaa gagatacggc gtagttcctg
     4981 tggtaactgc ttcagacgaa aattatgctc cttacttgag cgtgatgatc gcaactgcat
     5041 tagaaaattg caataaaaca agacgcataa aattttatgt gattgacgat ggtctatcag
     5101 aatatagtaa agaaggattg gaagaaacgg taaataaata ttcaagtaat gcaagtatcc
     5161 aattcctgac agtggaaaaa gatatctacg aagacttcct agtcagtgac catatcacaa
     5221 ctaccgctta cttaagaatt tctttaccaa acttattagc taaagaggat tataagaaag
     5281 tcctttactt agattcagat gtgttagtac tagacgatat cgtaaaacta tatgatgagc
     5341 cgttaaatgg aaaaacaatc ggcgctatta tcgatccagg ccaagtgaaa gctttggaaa
     5401 gattaggaat cgattccgat gacttgtact tcaattcagg agtgatggtg attgacattg
     5461 atcaatggaa taaaaaagaa attacagaaa aaactatcca ttacctcagt gaaaatggcg
     5521 atcgaatcat ctatcacgac caagatgctt tgaatgctgt actttatgaa gattgggaac
     5581 aacttcatcc aaaatggaac atgcagactt cgctgatttt tgaacgtcat ccagcaccaa
     5641 atgaaaaata tgaacgtcag tataaagaag gaaatgaaaa accttcgatc gttcacttta
     5701 caggacatga taaaccttgg aatacgttga aagatcatcc gtataccaat ttatacttaa
     5761 aaaaattagc acacagtact ctaacgaaag taggcgagat caatgaataa aaaagaaata
     5821 gcagtagtcg caagttgtaa cacaaaattc gtaccacatt tagctgcatt atttgtctca
     5881 gtattagata attgcaaccc gtctaaattt gttcggttct atgtgattga cgatgatatc
     5941 gattttgaaa gtaaacagct tttacgcttc tcagtgaaaa acgcacgaat gaactcagat
     6001 gtggaattct tgaaaatcaa taaagaattc tttacaaatg tggtaatcag cgatcgtatt
     6061 ccagaaacag cttattatcg tattgcgatt ccggaattgt tccgcggcac agaagttgaa
     6121 cgtatcttat atatggattg cgacatgatc gctttacaag atatctctaa attatggcgt
     6181 cttgacttcg gtgattcaat cgtagcagct gttgaagacg ctgggttcca tcaacgactg
     6241 gaaaaaatga aaatttcagc taaatcaatg cgttatttca actcaggttt gatgctgatc
     6301 aatgtgaaaa aatggttaga tgaaaatatt acgcaaaaag tattagattt catcgaacat
     6361 aatccagaaa aattacgatt ccatgaccaa gatgcattaa atgctatctt acatgaccgt
     6421 tggttaccac ttcatccgcg ttggaatgca caaggctata tcatggcaaa agcaaaaaaa
     6481 catccaacag cagcaggtga acgtgaatac gaagaaactc gcaacaatcc ttacattatc
     6541 catttttcag gacatgtgaa accgtggagc aaggattttg aagggccaac gaagaaatat
     6601 tacgaaaaat atgcagggat gacggctttc cgttgtgtag ctaaattccc taaatatcca
     6661 aaatatgcaa aagttcaacc aaaaacagat gtagttgtta aataatagaa gaccacaact
     6721 gaagaggggg agtaggaatg gaaattacaa tgtctaaaaa tgcagtagag acgttaattg
     6781 aaaaggttgg ggaaaataca aaaatcgcac tcgcgcttat caatgattcc gatccatttc
     6841 taagggacaa aggtgctttt gcaaaaggca gcttttttca aatcattcct tttgtttcgg
     6901 aatttggaga atatgcgact aaaatcgagc atccattatt ggacatctat acatctaaat
     6961 tagagcaaaa ttatttcggt aaacgattga acatggactt caacaaacag cttgacagct
     7021 tttcattaga aaatgaaatc gcagtactcg attataatat caaattaaag aactgcttct
     7081 tcagctaata gtttcgagaa agtccaaaaa ggaggtatac attgagatac tactttgtgg
     7141 atgaagaaaa gaatgtaata gaaacagatg aaaaaaaata taattggctc gtacttgata
     7201 gtgcaaagcc cgaagagatt gagcaagcaa tcgaagaatt cgagcttcct gatgatattt
     7261 tcgtaggaac cgaatatccg gaagaagttt ctcggatgga gcgcctttat gagacgaaac
     7321 tagagcatcc attttctttg gtagtgatta atctagatca gtcagataat cgtatcgaaa
     7381 aaaaattaac gccaatatct tttgtacttt ccgataatct cttgattact tgtatcgata
     7441 gaaaaaccga atttgtggat cggctgctga caaagcatgg aaatcattta aactcgtttg
     7501 aaaagatcat cacgtataca ttattagata tttacaccca ttacgtgaaa gaactaagag
     7561 agatgaaaaa gcgaattgac gctttggata aagcagctcg taaaacaacg gaaaatgaag
     7621 aactgtataa acaggcagat cttgaaagag acatcgtata tatcgatcat accttaagag
     7681 atcaacggga aacaatggat caattatgga attctgaaac tttcatcaaa cgtttagacg
     7741 atgaacagct actctatgat gtccatttga gacagcgaca aacagaaaaa atgatcgaga
     7801 tctatcgaga tttattagag agtattggtg atctattcaa cgggatgatg gacaataatc
     7861 taaatcatat catgaaatat ctagattcag ctgcattaat catttccgtt ccggcgttgt
     7921 tcgcagggat ttggggaatg aatacaggag gattaccggg taaaagttct gctctaggct
     7981 ttatactcgt agtctttggc tctttgatag cagcagtagt tttaggttac catttatacc
     8041 ggaaagatta tacaaattaa agaatctatt taaaacaggt atcaaacaaa gaaatttgtt
     8101 ttggcaatac aatttaaaaa ctaggaggaa tacagcaatg acagaaccaa aattaatcga
     8161 cccgcgaaac ttgtaccata cggagaaatt tcctaaacaa gatcaggata caccagcttt
     8221 acagcagaat atgattcctg ttcctgattg tggagaagaa tcctatgaag gcaataatca
     8281 actagaaaac cgccgtgtac taatcactgg tggagattca gggatcggtc gtgcagcagc
     8341 gattgcattt gctagagaag gggcagatat cgctcttcaa ttcttcccag gagaagaaaa
     8401 agatgcagag gaagtagcaa aatacgtgaa agatgcagga cgtaagattg tcttactacc
     8461 ttttgatctt cgtgacgaac aagcacctaa agaaatcata gataaagccg tgagtgaact
     8521 tggcgggcta gatacgatgg tactaaacgc aggacagcaa atttcttgtc agtctattgc
     8581 tgagttacca cttgagcaag tcaaagacac atttatgata aacatcatcg ctatgtttgc
     8641 tttagtgaaa gaagctgttc cgcatatccc agcaggagga gcaatcgtaa caacaacttc
     8701 tgttcaagca ttcaatccaa gcgaacattt attagattac gcagcaacaa aagcttcaat
     8761 cgctaacttt acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc gtgtaaatgg
     8821 tgtagcacct ggtccaattt ggacaccatt acaactagac gaaggacaac cttctgattc
     8881 gatccctgaa tttggtcagc aaagtttact agaacgtgca ggtcagccag gagaattagc
     8941 accagtttat gttttcttag catcgaataa agcaagttat gttacagccc aaatttacgg
     9001 agtaactggt ggagaagcga tcaacttata agaagttgat cgtttcaccc ctatgaggag
     9061 gtgcgaaaat gaataaaggt gtaaaaacga tcggagtgat cggaacatta cttttgctat
     9121 ctgtgctttt gtttgcgata ctattaagtg caacctatcc attgcctttt cagctagaga
     9181 agtttcgatt cttttccatc actaattcat acgtgcaaca atatgtattt tggcttgccg
     9241 ttgcatttgc tattttgaca attatcacgt tacttgtttt cctgttttat ccaaaaacaa
     9301 tcggaaactt tctactgaaa catggtgatg gggagttgac gttggataaa aaagcaattg
     9361 aaggaatggt tcgttctcac ttgcatgaag atgaattcat ccattcacca aaagtaaatg
     9421 tcaaagcaac gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa cgaacttcag
     9481 cactcattgg taaaacagga acattgatga aagaaatcga aaatgaagta caacacatat
     9541 tgggaacaaa agagccagtg aaagtatcgg tgaaatacac aggctttgaa gaaccagcta
     9601 aacgaagcaa tgaccgcaaa cgagtagaat agggggggac caaaaatgaa agaactattt
     9661 tcgacataca agctccctat catctttggg gtacttggat tgatattagc cgttttatgg
     9721 atcaccgtgg gctttttcaa gaccttattg atcttgatca tggcagcgat cggcgtcgct
     9781 atcggttttt atctgaaaca aacgaaaatc ttagatcact attttaaatc ttaggaggaa
     9841 acatccatga ataacaataa caaaaataat aacaataaaa tgaacaacca aaaaccagtg
     9901 tctgcacatc cagacccaac gacaccaaac caatcaaatg gtgtgaacga tgaaaagaac
     9961 aaaacagcac cagtatctgc acatccagat ccagtagtcc ctaacgttca acaaggagta
    10021 gaagagaaga aacatgatgc agaaaagcat gcacctcatc atccaggaac tccagaatct
    10081 aaagttccag aacaacatga tgcaatcgat ccttctaaac ctgtggaaga gcaaaaacat
    10141 ccttctgacc atgcgaaagc acaagaaaaa cttagttctg accacacaaa cggacaagga
    10201 aaacttggtt ctgatcatac aaataaccaa ggaaagcatg attctgaaca ggaagattca
    10261 atgcgcggtg agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg aatcgcaatt
    10321 gaacaagtag acggtttatt atctgcaaac ggtggtttct tctctaatgt ggcaggaaaa
    10381 cttgtgaata cagataatgt aacggctggg atcgaaacag aagtcggtaa aaaacaagta
    10441 gcagttgata tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat ctttgaagaa
    10501 atgcaagaag taatcggcaa agaagtaaaa aatatgacac atttagaagt gatcgaagtc
    10561 aacgctaacg tagtggacat caaaacaaaa gaagaatttg aaaaagatca agaaacggtg
    10621 caagacaaag taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa tcaaacagat
    10681 aaagtaaaat cagcagctgg aagcggcgca caaaaagtaa aagaaaatac agaaccacgg
    10741 gttcagtaat aaggaagtaa aacattagga ggaacgaaac atgggattta tttggtcact
    10801 tatcgtcgga ggaattatcg gagcaattgc aggagcaatc acgaataaag gttcttcgat
    10861 ggggattatt gcaaatgtcg tagcaggttt agttggatca gcacttggtc aagcattatt
    10921 aggttcttgg ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga ttggtgccat
    10981 cattttagta ctaatcgtat cattctttgc tcgaaaactg taaaaagact tttaaaaaga
    11041 ggtcgtgaaa aagctgttct tcatcaagta ataagaacag ccaaacaaaa atagcttctc
    11101 atatttttca tttggttggg catattatcg aagatgcaag cttttgaaca ctgtttatac
    11161 ggaataagag gctgtgacaa gacttttgtc acagcctctt atttatgcgg gcaaattccc
    11221 aattagattt ttaaaagttt ttccttaaat gtgtctttat tgatacagag aagcttcttt
    11281 aataaaacaa tgcctcccta aattttcttg agataggaaa ggtgaacata caaaataata
    11341 cgaccacttt gaaaacccag atgatccaca aatggatcgt atgatactga tgaagtaaaa
    11401 agcaatggat cagaaactca aagagattga aaaagacaag gaagaaataa tatctctttt
    11461 ttactcttgt ctgttttagc caataatagc tcgtaaagta atctagacaa acaaatagaa
    11521 gtaaacaaag tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga cgattgacga
    11581 gcaggaaaaa tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa aagtaaacaa
    11641 taaatggcat actattcctc ctaattgtgt gagttcgccc tcattttaaa ataacgatat
    11701 ttgtttgtga agctttttcg tgtcgtaaca aatgttatct aatgtatttt taaataaata
    11761 atcattatta tcaaaataat g
//
LOCUS       DAYYHS010000085        11779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000085 DAYYHS010000000
VERSION     DAYYHS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.085"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..201
                     /locus_tag="ACHA6A_002350"
                     /pseudo
     CDS             25..201
                     /locus_tag="ACHA6A_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            602..1207
                     /gene="fic"
                     /locus_tag="ACHA6A_002351"
     CDS             602..1207
                     /gene="fic"
                     /locus_tag="ACHA6A_002351"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8970514.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1223..1795
                     /locus_tag="ACHA6A_002352"
     CDS             1223..1795
                     /locus_tag="ACHA6A_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8970515.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2047..2304)
                     /locus_tag="ACHA6A_002353"
     CDS             complement(2047..2304)
                     /locus_tag="ACHA6A_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970516.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2430..3245)
                     /locus_tag="ACHA6A_002354"
     CDS             complement(2430..3245)
                     /locus_tag="ACHA6A_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970517.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3762..5087
                     /locus_tag="ACHA6A_002355"
     CDS             3762..5087
                     /locus_tag="ACHA6A_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8970518.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5080..5430
                     /locus_tag="ACHA6A_002356"
     CDS             5080..5430
                     /locus_tag="ACHA6A_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970519.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5412..5597
                     /locus_tag="ACHA6A_002357"
     CDS             5412..5597
                     /locus_tag="ACHA6A_002357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970520.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6501..6776)
                     /gene="prgO"
                     /locus_tag="ACHA6A_002358"
     CDS             complement(6501..6776)
                     /gene="prgO"
                     /locus_tag="ACHA6A_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8970521.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6748..7701)
                     /locus_tag="ACHA6A_002359"
     CDS             complement(6748..7701)
                     /locus_tag="ACHA6A_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8970522.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8313..9806
                     /locus_tag="ACHA6A_002360"
     CDS             8313..9806
                     /locus_tag="ACHA6A_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8970523.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9941..10237
                     /locus_tag="ACHA6A_002361"
     CDS             9941..10237
                     /locus_tag="ACHA6A_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8970524.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10338..11189
                     /locus_tag="ACHA6A_002362"
     CDS             10338..11189
                     /locus_tag="ACHA6A_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970525.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11210..11422
                     /locus_tag="ACHA6A_002363"
     CDS             11210..11422
                     /locus_tag="ACHA6A_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970526.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4051 a         1817 c         2181 g         3730 t
ORIGIN      
        1 atttaaaact ttgcaacaga accctattta ttaggaatgg ctcttgctta tagtgttggt
       61 tctttgataa aggtaaacgt gtcacttgat ctatttacta ttttactagc ggttggggta
      121 tcttcggtta ttggactggt cttttctgta atgcccgctt cagaggctgc aaaaaaagat
      181 ttaattgata ttcttagata aaatcagaat gacaagaata tttacgttct agattctaaa
      241 ataaaacaca aagagaccaa ccaggcaccc tattgtttct ttgtagtata gagatattct
      301 ttggcttgta agagatcaat agagttaggt gaccggagtt cgaaataaag gaaatttttg
      361 attgtatagg agaattcttt atccctaata tttagaattt tgagtatgcc atgttgatgt
      421 tttagattct ctataaatat agaggtgtat caaaggtcaa ctagactgtg caaaatagag
      481 acacttgaaa aatctatcgg gggtcagaaa cttaatagtt tttgacacat aaacaaaatc
      541 gcgatataat ggggttgaga gtgtttcata aacttatgtc ataaagttaa ggagatttcg
      601 catgatttta gagaataaat tgggattaac caatcaagtt gagttggcta aggttgaaga
      661 aaaactcagt aaacaaaaag cgaaagaatt gtacgattgt ggaaaaataa atgaaatcga
      721 agttggtaca tttaaaggtt tatctgaaat tcatgaattt ctattttcgg atatttacga
      781 ttttgccgga aaaatccgat cggtcaatat tgcaaaagga aattttcgat ttgcaccagt
      841 tatgtattta gaacattcgt tacagcatat tgatcagatg ccacaaactt cttttgaaga
      901 gattatcaag aaatatgtgg agatgaatat cgctcaccca tttcgagaag gaaatggccg
      961 aagtactaga atttggctgg atctcatttt aaaggaagaa ttgcaaaaag ttgtagattg
     1021 gaacttaatc gataaagcag actatctttc tgcaatggag cgaagtccca taaacgattt
     1081 ggaaatacgc tatctaattt ccaatgctct gactgataaa attgacgatc gcgagttata
     1141 tatgaaagga atcgatgtca gctatttcta tgaagggtat tctgagtata cgatagatga
     1201 tttgtaaaag taggtggacc aaatgacgaa atatggatat gcacgtgtct caaccgcttc
     1261 tcaaagtaca aaagaacaaa ttcaacagtt gcttgagaac ggcgtagaga agaagaatat
     1321 tttttcagaa aaatttacgg gtacaaaacg ggatcggcca aaattcaatc agctgttggc
     1381 tggtctgaag cctggagatg aaatagttgt tacgaagctt gatcgatttg ctcgtaacac
     1441 gagagaagca ttagagatca ttgaaccttt actcgatgat aacgtaacga tcaaggtttt
     1501 gaatctagga acgattgaaa atacgtccat ggggcgaatg gtgacacgga cgttgttgag
     1561 tgtagcagaa atggaacgtg atatgattgt ggaacgtacc caagaaggaa aaatgtttgc
     1621 aagaaagaat aatcctaatt tcagagaagg acgtccgaaa gcaacaatca caccgaagaa
     1681 gaggcacgca tatgaactgc ttacttcagg taaatcgtat aaggaagttg aagctataac
     1741 cggtttcagt cgcagtacgc tttttagaat aaagaagaaa attgaagaaa gttaaaaaag
     1801 aagtctcagc gaaccagaca ggtaaatact taatgcgttc tagatatgag tctattagca
     1861 tgttgtgaaa tataatattt ttttatttac ttgatttctg ctcaaaaaat gtgtatggta
     1921 actgtaaaag gtaaagtaat taaaggtatg ctagccacgc ttactatatt aggataccat
     1981 catatattta taatgaacga tttgttcgtc taccaaacaa cacatatcgt tcagcgtaag
     2041 tcacgtttag gaacgtggct tttttagtgt tattatcgat aaataatcta ttgactctat
     2101 tgattttatc tccatatccc tacggtgttg attgtcttta gatctttcat ttaagaacca
     2161 aataactatc ataaaaatca gcactgctgc taatataata aacagcattc ctattgtgat
     2221 attacctatt gattttacca taactacgat taaagtaata atggcattca agtctccttt
     2281 gttctctata tataaattag ccataaatca tcttcctttc aaattaagat acttgtttga
     2341 taccaataaa tagtaagcgt ggctagcata cctttttctg taatagcaag tgttctaata
     2401 gtccaaactt aatgatggac cgatgctagt tatagtacag taatgttttc tttcttatct
     2461 agatttttag taagctctat cagcaaatct aacaaatctg agtccttttc agaaactaaa
     2521 ttaaatctag tgtttacaac aaagttttca tgttctatat tgtttagatt atttgcggaa
     2581 aggagggctt ctagatcttg acattcaaat ctatatgcat cgtcataaat cttactagaa
     2641 ggagtgctta attcgaaatt acttattttc tcatcttgga ccaagataaa tttatagata
     2701 gtatcttttt gtgtttttct tttaaaaaca caacagagtg tgttataagg aacgttataa
     2761 ttttcatcag cctcattttt tctccattca aacaaatgat aaactgcaac caagttgcca
     2821 tcaatttcat ggacagtgct ctcggtaaca tcctttaaag aactatcaag ggctaattta
     2881 tagtaactag agagaggaag taaaattgtc gatttactga gatggttatc gtctatacgt
     2941 gcaatcatac ctaaatcaac taaggtttta ttccaggtgt ttatagtttt agttgtcact
     3001 cctagagact ctgctatggt attgaccgaa tgccaggaat ccccgccttc attctttgcc
     3061 ttaaaacagt aatacagata taaattaata gctttgttat tcggtaaata tttttcaagt
     3121 tctgttggta gaataaagta agaatcctta atgttttttc tgaactgtct ccagtctctg
     3181 tagttttgtt tataaagttg tgaaacggtt tttccacctt tagaaagtcc tgctatctta
     3241 accaatctta ttgctccttt cttgaatctg tcatgatcat acagtatttt ttacttgtag
     3301 tccagcacct taatatatta tcctatgtaa ttaatttcgt actagatagt aattattttt
     3361 tatttaattc tctagtcact tacaaataag ttatataaac tacgattaat aaattattac
     3421 aaaaactatt aagaaaactt ctacatacaa ctattacaaa actattaaga aactgaatac
     3481 tcctattttt tgtttttaag gaaagttttt tcttttttga atttcttatt tttttctaat
     3541 ataactaagc aaaactggat aatacgacac ttattttcaa attttcacaa gactaatagt
     3601 aaatatccat ttaataaagt gatacaatgt gttgatttta caaatcaaca gaagaagtag
     3661 ggaaataact tacctatgct tctttttttg tacatttaat cgattcttct ttacaaaaac
     3721 gaacgtatgt ttgtataatg tggaagaatg gagtgttatt tatgatggat tattcaaaag
     3781 agccagtaaa tgattacttt ttgatcgata tgaaaagctt ttacgcaagt gtcgaatgta
     3841 tagaaaggaa tttagatcca ttaacgactg aattagtcgt catgagtcga gcggataata
     3901 caggatcagg gttgatttta gcttcttcac cagctgcaaa gaagaagtac ggtatttcta
     3961 atgtcagtcg accacgagat ctaccgcaac cttttcctaa aacgttacat gtcgttccac
     4021 cacgcatgaa tctctatatt aagcgaaata tgcaggtaaa caatattttc agaaggtatg
     4081 tagcagacga agatttattg atttactcga tcgatgaatc gatcttaaaa gtgaccagat
     4141 caatgaatct ttttacgact gaaggaacaa gaagtcaacg gagaaagaag ctggctcaaa
     4201 tgatccagga gaatattaaa gaagagctag gattgcttgc tacagtaggt gtcggagata
     4261 atcccttatt agctaaactt gctttagaca atgaagcaaa acataatgat ggattccttg
     4321 ctgaatggac gtatcaaaat gttcctgata aagtatggaa tattcctgaa atgacagacc
     4381 tttggggtat tggatctcga atgaaaaaaa gactcaacca aattggtatc atgagtatta
     4441 aagacttagc taattggaat ccatacacta ttaagagcca tttaggtgtg attggcttac
     4501 agctgtattt tcatgcgaac gggatcgatc gaacagatat agcgatccca ccagaaccga
     4561 caaaggaaaa atcatatggt aattcacaag tgttaccacg agattatacc aggagaaatg
     4621 aaatcgaact ggtggtaaaa gaaatggcgg aacaagtagc tattcgtatc aggcaacata
     4681 actgtaaaac aggttgtgtc catttataca ttggcacgtc gatcttagaa acgagaccag
     4741 gtttttcaca ccagatgaag atccctataa cagataatac aaaggaattg cagaattact
     4801 gtcttttctt gtttgataag tattatgaag gacaagaagt aaggcacgtt ggaataacct
     4861 atagcaagct tgtgtacact gattcgcttc agctggattt atttagtgat ccacaaaagc
     4921 aaattgacga agaaaattta gataaaatta ttgacaagat tcggcaaaaa tatggtttta
     4981 cgtcgattgt acatgcttct tctatgttag aaggcgctcg tagtattact agaagtacat
     5041 tggttggtgg ccatgcaggc ggcgcaggag ggcttaaaaa tgattgatta taatgatcgt
     5101 ggacgtttaa agtgggcagg tttttattta tctgatcata ctgaaaaaat tgatgccgac
     5161 aatgaacaac gagccaatca aaatttggca aaagaacaaa tgacgactga agaggttgcg
     5221 gaagtattga acgctgcagt cataaaaaat agatcagtat gtattcaaaa agaagaacga
     5281 aacgctgaag gatactatcc agaagatatt gttagcaaga tcttaggtag tgatgagctg
     5341 gggatctacg ttggaaaaaa caaagttcac tacgacgaaa tacgtcatat cgaattttac
     5401 caggataata aatggggtcg attagaatga acattatcca gcaatatgag ctaaagtata
     5461 taacctttga tcagctatca gaagagattt ggggttatgg ccagcgtttg ataaatgagg
     5521 ttggtgttga acgcttctct ttctatgtag aagctgcagc aggataccat aaatacgaat
     5581 actatattgg ctattaaagt gcaataaaat tatatttctt ttatgtttta cattttgtat
     5641 caattctgat aaaattacct ataggtaatt aaaaaatata aaaaaactag ttacaacctt
     5701 aggacgttgt aactagcttg aagcatacta gagctaacta aaatagcact agaaagcaat
     5761 gttaaacccg tccaacaaac ttgatttaac attgagacca gcttattcag ctaaatatag
     5821 ttgaataagc ttcttttgtt ttaaaaaaac aaaaggtgta ctacgaataa cgcaagtaaa
     5881 agaaatacta ttacaaccag taggctacaa gtaagtatta ctaaacattg cgtagttaga
     5941 acctttgctc tattgtctaa agccacaaaa ttagagatca gctttatgat gctaagagcg
     6001 agctgcatcc atgcggacat aggttctttt gacactatta attctccttt ttaggataag
     6061 ggaaattgct agtagttttt tcattagcac catccccctt catttgattg agtctagtgc
     6121 tgctgtgata gtttgctgtt taatatgctt acattcttat tataacataa atgaaacaaa
     6181 cacatggtta gaggtcgtat caatcaaaat agtgtctcac aaacgattta ttacttactc
     6241 ctgctgacta acggactgtt cttcagtaac tgtaactata tgtttgttaa aaccaccatt
     6301 acctgtagat aaaaaccaat atatttacta tactcactat attaaatata tttagtatag
     6361 taaaaactag aaacaataaa atttgtgaat gttgtatacc cccaaacaat acacatattt
     6421 tgaaggatta ggattaataa agctaaaaat atagtaagta tactaaatat atttactata
     6481 tctagtatac ttactatatt ttaatctttt gatcgtctat tatatataat ccgttcctcg
     6541 tcgtttaaag tatcaacata ggcgtccatt gcaagtttga ctatttcata cataggaata
     6601 tcttttgcat aactaagatc ccgaatggtt tttagtacgg gaggatctac ttgaactggt
     6661 tttctttctt tcgcagtgta ctttcgttcc cctaactcga cattaatatc atctttaatt
     6721 gaaatatctt cagcaccaat tttcttctta tctttgttcg ttggtaataa tgccatttag
     6781 tttaatctcc tttccaagag gtaggatatg attggccatt gagtcagcat atttaggtgt
     6841 ataatcaaat ccttcaatat caccatgttg ttcatagtat tcaattcttt cttctaattc
     6901 tgtaaagatg tctgcaaaaa tagcccacca tcttcgatct acataatcat ttaattggat
     6961 accattctca ccatacattt ctaaccgatc cgagccttta acaatgcttt taaaaatgtt
     7021 tgattcacca tacttatcaa ttgttttttg atagtttgcg tgttgagagg aacgtcgcac
     7081 tgagaaaaga acaggcaaaa ttccaatgat ttgaagatga gactcttcaa aattcgtgac
     7141 aatcggaact aaaactttat taataaaatc ttctgttcct tccattgcgt accgttttaa
     7201 ttcttgcatt gcaataatat aatctgcagc agttaagaat gcacgaacca cattatctgt
     7261 tgatggtggg cagtcgataa agatataatc gaaatttttt ctcaattctt ctaaaggttt
     7321 aatgaaggcc aatgatctct tacgacgatc aggatatttt tcggacaagt attcattaag
     7381 ttcttcagat ccaggagagc cagccataat atataaatgt ggacttactt gagtaatacc
     7441 tgaaacccag tccccttttt tgattgctgc agacatggat cgatcaaagc tagtaatttg
     7501 atacgttgat ccaatgttgg aagtagtatt tctttgttgg tcataatcaa ttagcaacac
     7561 gtctttttca aaaagttgac tagaaacaat cgctaataaa tcattgtctg tagtcttacc
     7621 aatgccgcct tttctacctg taagcattac aacttttcct agtttatctg atgttttaaa
     7681 tgcagtccgt aagttttcca tagttccctc caatttccta tactaaatat atttagtata
     7741 cttcatatac ttaatatatt cgatatatct aatatactcc aaattgataa gaattcagac
     7801 tttataagga atatagtgga tatactaaat atatttgcta tattcagtat atcagatata
     7861 ttcaaaatgt caaaatatat ttgtgcgttt tgcgttgaca atttagagtg aaaagaggta
     7921 aacttaattc tatagaaacc acagataatt cactatttac aaagtagcaa aagcagtggt
     7981 actctttaaa cagaaaaatt acataaaaaa aagagacgga aataattgat tttagcagaa
     8041 tcaattattt ctgaatcaca tggttaggac atgtgttcac gctcgtctca agaatatgta
     8101 agtagctacc aactacttat attaaaagtt gatcggatac cttttccgat tgcctttatt
     8161 ctaccaaaaa aaggcgagca actcaacaga agaacgttta tttagtgtac ttaaattaaa
     8221 tattcttaaa cactgaagag agaacacata ctatggctga gattaaagct aaactgtgtt
     8281 ctctttttgt ttgagaaagg acgttacaat cgatgttgag cgaaatttat tcagctattc
     8341 ttaaaaacgg cttacgtaca tacaaatatc gaaacagcac catgaaacct gcaggtcata
     8401 ttgattccag taggagtggt gctatctttg cgtaccgtag caagcaatta atgaatgttg
     8461 ggcgtggtat ggtcattact tcggaagaag ctattttaga gaacgaaaag aaactaacgc
     8521 attggacacc taatacctat cgctttggta cttatgcgga tgattatcga acagtggtta
     8581 aaggtcattc tgagaaaaac ttaagtcaaa tcaacacatt tgtagttgat atcgattcaa
     8641 aagagaacca tcaaggcgaa attattttag cctgtttaga tcaagtgggc tacatgccaa
     8701 cgctaatttt agaatcagat catggttatc aagtttattt tgtactaaag acaccaggct
     8761 atgtcacaaa gaaaagcaat ttcaaggtga tcgatgtagc aaaaaaaatt tcaaaaacaa
     8821 ttcgtctaca gttggctgaa aaattgtccg gagtggatct aggatgtaac cacttcggaa
     8881 ttgctcgatt cccacaagaa aataatattg tttttaaaga aatgaactat cagtattctt
     8941 ttgctgactg gattcaatgg tcgatgaagc ttgaatcaga tcaaaaaaca aaagaagatc
     9001 gagaacgaaa actaattgtc tttcctgaga aaaaagaaat tcgtcaagta gatgaaccgt
     9061 ggttcgatat gttacttcat aaagcaaaca tcgtcggcgg agaaggacgt cttggtcgaa
     9121 ataatgtgat tttcacgctt tctttagctt attactcttc aggttatgca ttggatactt
     9181 gcgaatacaa tatgttcaca ttcaacgaac ggttacagga accgctatct gaagcagaat
     9241 tagtaaagat tgtacgtagt gcttattctg gtaattatca agcagcaagc agagaattca
     9301 ttattgaatt gtgccagcaa tgggtttcac caaccttaaa aaataatgat ctgttcattc
     9361 aacgacgtgg ctggtggaaa tttaaaaagc cacgagatca aagaaaatat agtcataaaa
     9421 atgaatggaa agaagatcta ttggcttatc taagcgaaaa aagtcttgct agtgagcctt
     9481 atttgaacct gtcaaaaaaa gaattggctt cacaactaac gatgcccaag cgaacacttg
     9541 ataagatctt aaatgagtta cgagaggaga ataaagtctt ctacaaggta acacgaggaa
     9601 gaaatggcgg aatgatcatt gcttcagtca aaacgttgtt cgcgagggtt attcaactaa
     9661 gtaaagaaaa gagactagat tattcaacaa ggatcacaga ggtatttgga ctcaacgaga
     9721 gcttagtaga agagacattt agacaattat caaccccctt gaaccctctt aaagaagtga
     9781 atctttttga agtagataca ggttgaaaaa taaaaggtgc aaattgcctt atacaatata
     9841 tctatgatac ttctgtaggt gttgctccct ttgcctagct acgtgtaggt ggtatactta
     9901 taactgcgta tgcagtctta tgtatcatgg aggaagttgt atgtcgttaa aaaattatgt
     9961 ctattcacat cctgttaatg tgtttatcat tggtcgtttg ggattatctg tggaagtgtt
    10021 ctgtgagctt tatggtttta agcaggggac tttgtcttct tgggttacca gggaaaaaac
    10081 agttgcttct ttgccgattg agtttcttca tgggttgtcc ttagcttctg gggagactat
    10141 ggatcaggta tatgattgtt tgtgtgtgtt agagcaggag tatattgagt ataaaatagc
    10201 taacgagtta agaaaaagga agaagtatat ccagtagtag tttttattta cttaaaaaat
    10261 tggttcattg agcaacctat cgctgctaaa gtagcaaagg aaaacgttaa aggaaagaat
    10321 ttagggaaga aatcgaaatg aaatcggacg actggattga acacacctgg cttcctctga
    10381 aaagaaatag caaacgaaag aaattttggg tattgcaatt atggccatta ggaaaacttt
    10441 gtagttacgg ggcacgactt attcttgatt taaccgttta ctcgattgga gtatttggaa
    10501 cggcttatta cttgataaat gatcccaata ggattgcagc atcagacatt tttacagtgc
    10561 taatgacgtt gatttttgtc atgctattgt ctatttttac tcaattagtc atgcaattta
    10621 ccaggcaaaa attaacagca ggaaaacttt tcaaaagcct attcgattta attgttgtaa
    10681 atgcaattct agcgacttta ataaaacaaa ttgaacaatc tggaagaaca acaaaaggag
    10741 tgaaggattt tttattgacg tttattcact ttgtaaatgg acacatgtat ttgtttgaag
    10801 tgggtattat tctctttttt gtcgggtatg tatccacgaa acttattaaa cgttcaattg
    10861 aaaaagaaat tgaaattgac ctgattccga cacttcttgg ttatgagtac aatgttgatt
    10921 ttggaagata tgacgaattg atcaatacgg atatcgttca cacgagtatc catgaatcta
    10981 ctgttaaggg catcagatct attttaaaaa atggccagga aatgaaagca atagatcgaa
    11041 gaaaagagat ctacaatttt tatgaagtcg aagcaagcct acgatatggc atcggaaaat
    11101 tgtggttaga tggaagtaat attcgtcaaa acaaaccata cattttttgg aacagtagtg
    11161 atttagaact tccaatgaaa gataaataat tatggagaat cgtttgaaaa tgattcctct
    11221 atacaggatt gtgcttatag ttggcatgga gttaaaaacc ttaaagaagg agcagttatt
    11281 caaagtgaaa gaacgtcata atcagttact atgtagtaaa acgggtacga tgaacattga
    11341 ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgttga ttcgaaagca
    11401 acaagagcaa gaaaaaaatt aatggctact tatagccaat tgattgatga atgggaacaa
    11461 ttcaaggaat ctaaaattcc agaacgtctc taaaagcctt tatttgtaaa gagcataagg
    11521 aaacgtggga acacttttta aaaaaagtgt tccaataggc tgtgcctatt acgtttctca
    11581 aaaataaatg tgggaacaaa atatttaaaa ataggatagt gaaaggatag tgaaggatag
    11641 taaaaggagg gtagtaggat agcaaaaagg ataggctcct tttcgctatc ctttttaaat
    11701 tggaggaatt aatatggaag taagaataag aaatgtggat gagaaaatcg taagagaaat
    11761 tgatcgaaga tgtgacgaa
//
LOCUS       DAYYHS010000086        11700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000086 DAYYHS010000000
VERSION     DAYYHS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.086"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            451..1920
                     /locus_tag="ACHA6A_002364"
     CDS             451..1920
                     /locus_tag="ACHA6A_002364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970527.1"
                     /translation="MSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTS
                     DNIVLLYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGI
                     YNSSNTPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYP
                     TTTRQDYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTT
                     IDSAVISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADK
                     IAANILTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISG
                     HSDWRGLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYL
                     PSMLRIGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIR
                     EICAKLAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            1930..2097
                     /locus_tag="ACHA6A_002365"
     CDS             1930..2097
                     /locus_tag="ACHA6A_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970528.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            2084..2491
                     /locus_tag="ACHA6A_002366"
     CDS             2084..2491
                     /locus_tag="ACHA6A_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM8970529.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            2492..2911
                     /locus_tag="ACHA6A_002367"
     CDS             2492..2911
                     /locus_tag="ACHA6A_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8970530.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            2901..3809
                     /locus_tag="ACHA6A_002368"
     CDS             2901..3809
                     /locus_tag="ACHA6A_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8970531.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            3925..4962
                     /locus_tag="ACHA6A_002369"
     CDS             3925..4962
                     /locus_tag="ACHA6A_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8970532.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(5023..5328)
                     /locus_tag="ACHA6A_002370"
     CDS             complement(5023..5328)
                     /locus_tag="ACHA6A_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970533.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            5623..5796
                     /locus_tag="ACHA6A_002371"
     CDS             5623..5796
                     /locus_tag="ACHA6A_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8970534.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            5857..7410
                     /locus_tag="ACHA6A_002372"
     CDS             5857..7410
                     /locus_tag="ACHA6A_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8970535.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            7407..7823
                     /locus_tag="ACHA6A_002373"
     CDS             7407..7823
                     /locus_tag="ACHA6A_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970536.1"
                     /translation="MNNTIPYGYVIKNGEINVDEQQAQQIREFFNIYLEGVGLQEAGK
                     RSGINKPHPFMKRILMNEVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVP
                     DTKVIPTHFKSGKITEKYSDPFKQAQYAYSKIKEVK"
     gene            7825..9375
                     /locus_tag="ACHA6A_002374"
     CDS             7825..9375
                     /locus_tag="ACHA6A_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8970537.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            9498..>11700
                     /locus_tag="ACHA6A_002375"
     CDS             9498..>11700
                     /locus_tag="ACHA6A_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970538.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKN"
BASE COUNT         3885 a         1989 c         2596 g         3230 t
ORIGIN      
        1 actagtgatg caccagaaac catcagttgc tatatgttga aaacggatgt gacaaatggc
       61 tatgttgatt tatccgagat aagtaacgtc gtaaaactta cagcgaccac tgatgcctta
      121 ctgttaagtg tttcagaaac caaagaagca atcggcgttc catttaaagt caatcaatgg
      181 gaacaaggaa gtttgagtac cactgacggt tcagaagtgt ccgcaaccaa ttatctccga
      241 agtgagttcc ttgatgtcaa agtgggagat aagtatatcg ggcaactgcg tgacggaact
      301 caagctgctt tctactatca ttactacggc atggatttac cttacattga ttttgtccca
      361 acggcaaaac aatttagtga aaccctaacg gcgggaactt atactaatgc gaccattgcg
      421 agttatctag taagtaagaa cgttgaatcg atgagtatta caagtggcgt gacaacaaac
      481 ccctattcaa attcaggaga ttatctggtc atttacaaat tgccattgga aggcaaagtc
      541 gctccgagtg taatttcgtg gaatggtcgc aaggatacat cagataatat tgtcttgctt
      601 tatacaggtg gtgcttggga acagatttcg accgttacaa gtagcagcaa tgccacttat
      661 tcttggacgt taacggctga gcagatatca gggattacta ctgacagcgt gtatctagct
      721 ttcttttcag ttaagactgg cacatatgct gggatttata attcaagcaa cacacctttt
      781 actctaaacc cgtcggatgg ttcaagttat catcaaatct cttatactgg ttcgagcgga
      841 gcaattacag tcccgagtgg cacattgaaa atgcgagtcc gagtgaacac gagcatcaaa
      901 cccgatgaat atgaaggaaa tatttatcct acgaccacaa ggcaagacta taccaatgcc
      961 aacacattat attcagcaat tctcatgcag aaagacttaa tcaatttacg agttggtaaa
     1021 ggagacgtga ttaatcaaat caatatctca cctgaaagta ttttgattgc tggaaacaag
     1081 gtgcacatca ctggtcaaac aaccattgat agtgcagtga ttagttctgc catgatccaa
     1141 aatgcggcca tcacaaacgc aaagattgca gatgccacaa ttcaaagtgc aaaaattgca
     1201 agcattgatg cggcgaagat taccacagga actttgtcgg cagcccgtat tggcgcgaac
     1261 tcgattacgg ctgataagat tgctgctaat atcctaacag cattgactgc gagttcgtcc
     1321 attcgaatca caggaactac gattggatat tattcaggaa gcactcttgt gactgaaata
     1381 aactcgcagg gaatgaccat tcgacgtggt ggaacgacag tcggaacaat tggtgcaaat
     1441 aacatcagtg gtcactctga ttggcgtgga ttggtatttg atcttgagta tggaacagag
     1501 tatatgtcat gggctcataa agattcctcc tctgcgagtg tctacaccca gaagctttct
     1561 tggtatgcga gaacattgca gtctggtatg gagaaggggt tccactttga cgacgacatt
     1621 tatttaccat caatgcttcg tatagggaca caacatcaaa tatctgttgg tgggattaca
     1681 tttagtagtt ccctttatac taagattggc tcaagttccg gcagcgctgg tattgcattc
     1741 aacggttcta atctgatgct cggagatgat gggaagtggg ttgactttgg agtcatccgt
     1801 gaaatctgtg ccaaacttgc gggacgaaca atcgctttac caaccggttt cagtagcaac
     1861 ggtacagcga gtggttggta caatccgcag tcgtttaatt caatgaccac atacagttag
     1921 gaggccgcca tgaacgaaga aaaagaagaa gtgatagtaa ctgaacctat cgaggcagat
     1981 attccaaagc caatgaaagt tgggacggat atggttgatt tgcttgtgat ggtcgtggat
     2041 gatctttgtg tccagattgg tgatttaaag gagaagatag ataatgctca aaattagaaa
     2101 tgaagatttg gtggacgtga tgagtttctt aaaaggtttt gacttaccac caaagattag
     2161 ccgagtgcga acgaagcttg caaaactcat ccaggtgaag gttgatgaac tctatcaaga
     2221 cgaggtagac ttgcttcaaa aatacggaaa gaaagatgag cacggcaatt tggtccaaca
     2281 tgacggtgcc ttttcactca tcccagaaac cgcaactgaa tatcacaaag aaaaggcaga
     2341 cttgctcaac gaagaaaccc acatcaacgt ggatgaactt caagacaagc tgcctttatt
     2401 ggttgctgga tttgaaaata gtgaagtcac catttcaggg aaagatgctg aaatgctcga
     2461 tttaatttta gaacaattgg aggatttgta aatgaaagaa gtgtggaatt ggattcaact
     2521 cgccatcgca ggtggtggag gagtgattgg atggtatgtg ggaggaattg atggtttgat
     2581 ttatacgctt gtaacttttg tggtagtgga ctatgtaact ggtctgcttc gtgcaatcaa
     2641 cgaaaagaaa ctatccagca agattggggc aaagggcatc actaagaaaa tcatgatttt
     2701 tcttttggtt ggtgtggctc atatgcttga tgtggaagtc atcaaaggtg gaaatgtact
     2761 gcgtgatgcg gtcattttct tctatatctc aaacgaagga atttcactgc tggagaatgc
     2821 cgtggcgatt ggactgccag tgccggaaaa gttgcgtgaa atcttgcagc agttacacga
     2881 aaagaaggag gaaacagaaa atgggaatta gtaatctagc aacaaaatat ggatttcagt
     2941 catttagcca ttactcgaat ggtcgtagtg gtgccaaagt agataagata gtgattcatc
     3001 acatggcaac caccaactat gatattgttc ctgatatttg gaaaacgcgt gaggcgtcag
     3061 cacactacgg tattggaaaa aatggagaaa ttcgagcata cgtggacgaa ggaaacacag
     3121 cttggcacgc tggtaattgg aacgccaata tctcaagtat cgggattgag cactgcaata
     3181 gcactggatc accgaactgg ggtatcaatc aagcaactat tgatgcaagt gcaaagttgt
     3241 gtgcagatat tgccaaacgt tatggtctag ggaaattggt ggtgaacaag aatatcttcc
     3301 ctcactcaca tttttcggcc acatcatgtc cgggacagtt gcttggtaaa ctgcaggaga
     3361 ttgcggataa agccaatgcg attaataatg gttcatcaac cccaagcacg ccagctagtg
     3421 gtctgtatcg tgttcgcaaa tcatgggctg acgccaagtc acaaattggt gcctataagg
     3481 acttgaacaa tgcgaagcgg gctgcagata gccattctgg ttataaggtg ttcgaccaga
     3541 acggtaatca agtttatcca tctgggtcat caacatccaa cgtgaaaact cttgtggttc
     3601 aagtgagtgg tctcaacgtt cgcaacaagc caagtttaag tggtgcggtt gttggttcat
     3661 acaataaagg cgcaacttgg acgcttccga agtcagagaa gatgacgatt gcggatggtt
     3721 gggtatgggc tcgttgcccg attggatatt gtgctgtcgg taagaatact ggtaagacgg
     3781 aatctgacga ttatattttg atttcataaa tttaatcgga acaaaattat gtccggattg
     3841 ttgacttaaa atatcgaacg tgttattatt aattcaacca gaacataaat ttgttccgat
     3901 tgaattatag aaagaggtat cgatatggtt acgtttaaag atttgttacc agaaccaaca
     3961 gaaagccaac aagctttcat tttagatcac gggaaaacag aagatgatgg tcagttgaaa
     4021 tacgctgatg atgctaaaag ttacggttgg aacatgcgcc aatatggtaa attgaaagcc
     4081 ggcgcggttg ttttgaatcg tcatccgggg aaaattacta aagaccgtaa gtgggaaatt
     4141 tacgggggcg gatacgttga gagcatttct gaacctgatg aagctggaaa tgttacggca
     4201 gtaattacac acgcatttac aatcgaaccg gcaattaaac aaggtgattc atttattgaa
     4261 aacttcgatt ggaatacacc tagcaaaaag aagcgtaaaa agccgaattc atgggcctac
     4321 ttctgggatc agtatggaat gaatgaaatt acctatgccg attttgttgc attgattgaa
     4381 aatcgtcatc ttatcccaat cgatgataca caatcgttgc cgtctgaact ggatcttaca
     4441 aacgaggaag ttgaagaaat tgagcaagaa agttcaaaag gatttactgt tcttgttgat
     4501 gaagtgggac caaatcgcac gaatgggact cagaaacgaa aattcactgg acgccatact
     4561 gattgggagc gtgtcaacaa ggcaaaacaa aaaacgggtg ctctaggcga agaaatcgta
     4621 cttgatttcc tcattcagga agcagaaaag aacaaaacaa aacttcctga acatgtttct
     4681 aaaactgaag gagatggtca tggctatgat atttgtgcgt ttgaccaatc aggtaatgaa
     4741 atccatattg aagtgaaagc ttcaaaaaca aacttctctg acggatttga aatgagtact
     4801 aatgaggttg ctgcctccct tggagataca ccatataaaa tttactttgt tcacgacctt
     4861 gatgtggcgt caaaagtttg caaaatcaaa atttatgatg gcccaattac tgaagaaaac
     4921 ttcacgatgg ttccaacgag ctacaaaatt ttcaataagt agtttgacgt ctttataatt
     4981 caaaacagcc tcacaagttt tattgtgggg cctttttttc gtctagtttt ctttaaggta
     5041 ggtgtttaca gcattttgca ttaggattaa atgatttctt aactgaaata cctgctcttt
     5101 gtaattgatg cagaaaagag gcgattctat tgattccata cgattattca tcgcgtttat
     5161 attggcttgt aaaaactggt ataaagcttc ttcagcctct atggttttaa agcacttttc
     5221 aaattcttgt ttgaagcttt catcaaattc ctccagttcc tttgttgtcg cagatttgca
     5281 aaaatgttca tattcttttt ctaacccttc catatcaaaa ctactcataa ctttctcctt
     5341 cccacaattg atatttatat tgttatttat tggatgaagc ttgtaaaggc ttatttgatg
     5401 tttgggaagt aaaaagtcaa aatttacaaa gaataattcc tactatatat aaacaaactt
     5461 agaagcctgc aagagtgatt cgtttcattc ttgcaggctt ttttcgtttt gtgggttcga
     5521 gtcggttatt tctttcgcat atggatgaaa ggcaaaggaa aatttgtgat tgggtttact
     5581 ttcatggatt ttcttcgcct gtatcttgag gaggcgaaag caatgactaa caagaaaaca
     5641 gaaattaact attcagatga agcagcctat tacatttcac agcagatcct tctgaactta
     5701 cttgttaccg gtaaaatcaa cctggaagaa tacgtcaaaa tcgacaataa aaaccgggaa
     5761 acattcaatc cttttttatc aaggataatg gtctgaaacg ttgatataac ggcactttag
     5821 agctaacatg tcacctaatg gaaaggagtt gagctgatga aaacgataca taaattggca
     5881 cgtccagagt tgccggaagc ttccaaacta aaggtggcag catacgctcg tgtatcaaca
     5941 tcaagtaatg aacagctggc aagtttacaa acccagatta ctcactatga aaatcacatc
     6001 aagaaccatg aacaatggga ctatgtcggt gtttattatg atgaagggat ttctggaacc
     6061 aagactgtaa aacgtgatgg tttaaaacga ttgattaagg atgcagagct tggaaagatt
     6121 gatttgattc tgacaaagtc tatttccaga ttttcaagaa atacagttga ctgcttgaat
     6181 ctggttcggc agctaactga tattggtgtc accatctttt ttgaaaagga aaacattaat
     6241 actggtgata tggaaagcga gttgttgctt tcaattttat caagcttggc agaaagtgaa
     6301 tcctattccc attcagaaaa cttgaagtgg gcgattcgaa accggatgtc taaagggaaa
     6361 ttcaaagctt acccgccgta tggctacaag cggaaaggct atgattttca catcgttgca
     6421 gctgaagcaa aagttgttaa acaaatgttc cagtgggcat tagatggcat ttcgactgga
     6481 atgattgcca agcggttaaa cgatggaaaa caattcacta aacgtggggc aaattggcaa
     6541 gcaacatcag tcagaagtat tttgaaaaat gaagtttata ccgggacttt gacgatgcaa
     6601 caaacctttg ttgatgacca gtacaaacga cgcataaaca agggcgaatt gccaatgtac
     6661 cagatcaaaa acaatcacga acccatagta tctaaagaag attacgaccg agttcaagaa
     6721 ctcattcgga gaaaagctga ggccaaaggg aatactggtg acgggaaaaa gtacaccaag
     6781 cgatacgcat tttcaggcaa catcatctgt gataaatgcg gcagcaaatt caagcgggtt
     6841 cacacttatt ggaaaggtaa tacgaagaag gttcagtgga agtgtacggg gcatcttaaa
     6901 aataaagata gctgcgatgc attaccaatt acggatgaag atttgaaggt tgcttatttg
     6961 accatgttga ataagttgat tttcggacac aagttggttc tggaaccttt tatgaagtct
     7021 ttgaataaag caggtgcgaa tcaggaaggg ctcgaagtgc ttgctgatga aatcgacaag
     7081 attcaagaaa agcttgaggt tctggcaagt ttgactgctt ccagtatttt atcaacgaag
     7141 gtgtctttag aagaacaaag caaacttcaa caagagaggg ctgtcgcaca agaaaagcag
     7201 cgcctcatca ttgagtcagt caacggcaaa agcactcagc aaattgaact agaagcctta
     7261 tacaaattcg cgcggatggc agagatgttg acggactggg atgaagattt attcaagaag
     7321 ttcacaaaca gcattacagt ttacaaccgc caagaagtcg gatttgaact taagtgcgga
     7381 ctacttttga aagagaggtt accagaatga acaatacaat tccatacggg tatgtcatta
     7441 agaatggaga aatcaacgtg gatgaacagc aagctcaaca aatacgtgag ttcttcaata
     7501 tttaccttga gggtgttggc ttacaagaag ctggcaaacg ctctggcatt aacaagccgc
     7561 atccctttat gaaacgtatt ttgatgaatg aggtttactt aggaaatgag atatatccaa
     7621 aaatcattga tgaggatatt ttcttccaag cggatctgga gcaagaacga agaattaagt
     7681 tctttaaaaa acgcaaggag atcaaagtgc cagatacgaa ggtaataccc actcatttta
     7741 aatctggcaa gattaccgaa aagtattctg acccattcaa acaagcgcaa tatgcatatt
     7801 caaaaatcaa ggaggttaag tgaaatggca ggaaaacagg tgacggttat tccaatgaag
     7861 cctagacaat gggcagcaga gaataccgaa gagaaaccaa agttgaaagt ggcagcctat
     7921 tgtcgtgttt ctactgaaat ggaggaacag gcgagttctt acgaagcaca ggttcaacac
     7981 tatacggact acattcaaag aaatcccgat tgggagcttg taggaatatt tgcggatgaa
     8041 gggatttcag gaactgggac gaaaaagcgt gacggtttta accggatgat tgaggcgtgt
     8101 caaaacggcg aagttgagta catcatcaca aaatcaattt cacgctttgc ccgtaatacg
     8161 gttgattgct tgcaatacat acgacagtta aaagaactga agattgcagt gttttttgaa
     8221 aaggaaaaca tcaatacaat ggatgcgaag ggtgaggtac ttttgaccat tatggcaagt
     8281 ctggcccagc aagaaagcca gtcactaagt caaaacacca agatgggcgt acagtatcgt
     8341 ttccagcagg gacagctacg tatcaaccac aatcattttc ttgggtacac taaggacgaa
     8401 gatggtaact tggtgattga accaaaggaa gcagaagtca tcaaacgcat cttccgcgaa
     8461 taccttgagg gcagttcctt gcaagaaatt gctaatggtc taatggctga tggcatccta
     8521 acgggaggga agcgaaagct ctggcgagga gaaggagtca gattgattct tcgaaacgag
     8581 aagtacatgg gggatgcgct gcttcaaaaa acatatacta ccgatttctt gacaaagaaa
     8641 cgagtgaaga atgacgggag ttacgctcag cagtattatg ttgaagatag ccatccggcc
     8701 attatcccaa aagatatctt catgcaagcc cagcaagaac tagaccggcg taaatcaatg
     8761 aaaaacaaaa agtcacagtg cttttctggg aagtatgcgt tatctggtat cacagtttgc
     8821 ggtgactgtg gcaatgctta ccgcagagtt cattggaaga atcgaggaac tgtgtggcga
     8881 tgcaaatcca gagtggataa acgagaacat aattgcacgg gtcgaacaat ctacgaaaca
     8941 gacctgcatg gagctgttat caaggcaatc aatgaaacgt taatcgatcg tgatgctttt
     9001 cttcagcaat taaatgacaa tatcaactca gttatgactg atggattaac tgaccgattg
     9061 gcagaactag acagtcagtt gaaagagctt gaagctgaaa ttattagcct ggcagttggc
     9121 ggtcagggtt acgatgaatt ggcaagccaa atattttcac taagagatga acgagatgcg
     9181 gttgcaaaac aaatagcagc gaatactaat ttgcaacaac gggttgatga gatggtagtc
     9241 tttgtaaaag aacacgatgt catcaatgag tataatgaag tgcttgttcg aaggttgatt
     9301 gagaaggtca ccatttttga aaagaacatt gttgttgatt tcaaaagtgg ggtgagagtg
     9361 actgtagaaa tttaatttga atagatagga ctagacgtta atgtgggtag ccatattaac
     9421 gtctttattg attggatttc ataaagtgat tgatgatata atatattatg taaaaaatag
     9481 gctgggggcg aagttctgtg agccaatgga ataccattga tgaattaagg gatattttta
     9541 ttcgtaaata tacggattct gagttgagta atgaacttga aaaggcctgc agcgaagaat
     9601 atgaattgat gcgtgattat aatgggcgtc agattttaga gttattacag aatgtagacg
     9661 atgcctatgg agatataaaa tcagaaaatg atgctagcaa tgacgaagtg aaagttcaga
     9721 taacatataa aaataatatc ttggaagttg ggaatacagg cacttcattt tcaaaagata
     9781 caattgaaag attgtgctta ggaagagcga gtaataaatc atcacaaaat atcggaaata
     9841 aggggactgg ttttcgttcc ttgctaaacg atgctgagtg ggtcgaactg tattcgggag
     9901 aatttgctat tcgatttagt gaagaattca caaaaaagct ttttgagcag tatgtatcaa
     9961 gagattctga caaattcagt gaattaattt ttgagcagaa gaaaaattgg aaaaaagaag
    10021 attatgattt atgttttcca ataatgaact gtcctcaacc aataaacaaa attgattctg
    10081 gttttgatac attaattcga gtaaaactaa aagaaactaa tttaagtaaa ggcacgagtg
    10141 tttctaaaca acttcagcag ccattttata agtcactgtt atttttacca aatataacaa
    10201 aaattgttat tgaaacaact gatgatgaaa aaatatcaga gaaaattgtg gatggaaatg
    10261 atgtattgat agaaaaaagt gatgttactg gtcaatcacc gtctgaagaa tattttgttt
    10321 ttgacaaaga atctatgata ggagataaag ttgctaaaat tgcactagcc gtttctaaag
    10381 attctgatta cgattactcc aaagaaaaac tatattgcta ctttccagta cgtaattttg
    10441 gtacaccaat tcatgcactt ataaatgcac cgtttataac aaataattca cgtgatgacg
    10501 tcccggatga tagtgaacaa ataaataaga aaatatttat tgaggtgctc aaatttattt
    10561 ctgaagtagc agaaaaatta tctaagcctg aatatgaaga catagcatta aaaatggtaa
    10621 ctccttttaa agagaataaa ctatgggact tagatgtatt tgacttaatg aacgaattct
    10681 tgggtatttt atctaatgcc caaatactac cgactgtcaa ttggcaatat atttctatca
    10741 aagataatcc aaaacttttc cttcaagaat tccctgagga gctaataggt gagacatttt
    10801 ccaccttgtt gcaattatta gaaacaaacg aaaatgacca actagtacaa gaacttgcta
    10861 attataatga atacactggc ctgtcgtttg ttgtagatga actatctgat aaaatcaaca
    10921 ttctttcatc ttcttgggaa aatagaataa ggacaaaagt gttcttatgg tggagtgagc
    10981 aatataaaag ttctccgatt gtccctaagc tattaaagga caatagtgac aattggttga
    11041 tgaaatcaga taaggtattc ttaccgaccg atacaggtgt tactgtgctt ccaaaagagc
    11101 tgtcttcttg ggttagttta agcattttga aacaaacata tgttgatgag ttaatttatc
    11161 agattaagac ttcatcaagt tggaaaaata aatgggaaga aacagctaat gcatacaaag
    11221 ctgaacgtac aggagataaa cgtattctag atgcattcag tgaaaaatat cttgcaattg
    11281 aattcactga gcaaagcagt tcagatttga tcattggaac gattaatcga caaattgaca
    11341 ctgttgaaaa atctatcagt ttcattaatt ggttttatga taactacaat gaaaaactat
    11401 cagctggaag tgagctgtct aaagttccat ttaatcttcc aaacgttgct ggtgacgttc
    11461 aaccttccaa taaaatgtat attggaaaag agtatggcaa tgaattagga gataagcttt
    11521 ttcaagatac agattacacg gcattgaaaa aaatagattg ctctagtttt gctgaggaga
    11581 gagattttct gtcatttatt cttcgatgtg gtgtttccaa atatcctaag atttatgata
    11641 acgattcttt aaaaaatgat gttggattta aaaagtttat ttataacaaa tataagaatg
//
LOCUS       DAYYHS010000087        11697 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000087 DAYYHS010000000
VERSION     DAYYHS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.087"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..2026
                     /locus_tag="ACHA6A_002376"
     CDS             8..2026
                     /locus_tag="ACHA6A_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8970539.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2096..2302
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2453..4867
                     /gene="leuS"
                     /locus_tag="ACHA6A_002377"
     CDS             2453..4867
                     /gene="leuS"
                     /locus_tag="ACHA6A_002377"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8970540.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5215..6768
                     /locus_tag="ACHA6A_002378"
     CDS             5215..6768
                     /locus_tag="ACHA6A_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8970541.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7149..8933)
                     /locus_tag="ACHA6A_002379"
     CDS             complement(7149..8933)
                     /locus_tag="ACHA6A_002379"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970542.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8948..10669)
                     /locus_tag="ACHA6A_002380"
     CDS             complement(8948..10669)
                     /locus_tag="ACHA6A_002380"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970543.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10769..11530
                     /locus_tag="ACHA6A_002381"
     CDS             10769..11530
                     /locus_tag="ACHA6A_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8970544.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3812 a         2142 c         2376 g         3367 t
ORIGIN      
        1 atagaagatg gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt
       61 tcgaaaagca aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc
      121 tgccccatta gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc
      181 aagaatcaat acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca
      241 agccaacgat ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg
      301 cagtagtgcg ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca
      361 aggacaaacc gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa
      421 ggagcctttc cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact
      481 tagaaatact tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa
      541 atcctataca gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc
      601 aggcaaatta aataatatca tcgcaactta cggattgacc caatatgata caccagctag
      661 cggaaacgca ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac
      721 tggcggttca agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac
      781 tgtccaatct ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca
      841 attgatccaa tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat
      901 caaaggtagc caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc
      961 aggaaatgct ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc
     1021 tggtgattct gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga
     1081 atggaacaat atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg
     1141 cggttcttct aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag
     1201 ttcgtccagc tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg
     1261 agactctgtt tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg
     1321 gaacaatatc caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg
     1381 atcaacaaac agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc
     1441 agttaatact ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt
     1501 ttggagcgtg gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat
     1561 caaaaacaac ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa
     1621 tacaaataca ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac
     1681 gacaagtaca tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg
     1741 gagcgtagcg aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa
     1801 aaataatttc atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac
     1861 aaaccaacaa tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg
     1921 gagtgttgct gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa
     1981 gaacaacttt atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa
     2041 gaaataacga ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa
     2101 cacttgaaaa ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat
     2161 gtgcacggat gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa
     2221 gcggcggtga cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta
     2281 ccgcgtagat tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt
     2341 gtgaagtttg agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt
     2401 cgttatattt ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta
     2461 caatcataaa gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa
     2521 cacccatgat gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc
     2581 cggtcaaggg ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg
     2641 tgtgaaaaga agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct
     2701 gcctgctgaa caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa
     2761 tatcgaaaca ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga
     2821 agtgaataca actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta
     2881 tgaaaaaggt ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac
     2941 agttatttca aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt
     3001 cagaaaacca atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga
     3061 tgatctagaa ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg
     3121 tcgttcagta ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt
     3181 atttacgaca cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact
     3241 agatcttgtt agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga
     3301 agagacagct aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt
     3361 atttactggt gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc
     3421 cgattatgta ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga
     3481 acgtgacttt gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg
     3541 agatgttgaa aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga
     3601 cggcatgaac aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt
     3661 agggaaaaaa gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg
     3721 gggtgaacca atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga
     3781 agagctacca ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc
     3841 tccattagca aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg
     3901 ccgtcgcgaa acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta
     3961 tatcgaccct cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc
     4021 agttgacatc tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt
     4081 ctggcataaa ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact
     4141 atacaatcaa ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa
     4201 tgtagtcaat cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga
     4261 aatgttcatg gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag
     4321 ccgtaaattc ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga
     4381 ccgtatcact acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa
     4441 agtgacagaa gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt
     4501 tatcaatgaa gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca
     4561 actactcgca ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga
     4621 tggtggtatt tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga
     4681 agtagaagtt gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga
     4741 cttaggaaaa gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat
     4801 tgaaggcaaa acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc
     4861 taattaattg gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca
     4921 gaatgaactt cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca
     4981 aaaaggtttt gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct
     5041 tgtaagagat ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta
     5101 taaactacct gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag
     5161 tgattgtatg gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat
     5221 caagaactat ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa
     5281 aaaagagcga aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt
     5341 tttttcggag ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa
     5401 gggatgcaaa aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt
     5461 gttgtgctta ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat
     5521 ttcgatcaat ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa
     5581 gatgatgact atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa
     5641 agtgattgcc agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc
     5701 aaatcaaaat tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg
     5761 gaaaaaagcc agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact
     5821 ggttttttcc tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa
     5881 atgtcttctc atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat
     5941 atacaaaaga caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca
     6001 caaaagataa atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat
     6061 agtggtgcta attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac
     6121 accctgacaa cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc
     6181 gccgatttct catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga
     6241 gataatggta aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat
     6301 cgaaaagcgg tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg
     6361 tctgcagaaa ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt
     6421 ggaagtacaa tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt
     6481 tttgtttcga cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa
     6541 tcagttctgt cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca
     6601 aatgaagtta ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa
     6661 ggaccaatcg atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa
     6721 acatggcaat tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt
     6781 gtgagcctga cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat
     6841 ttgttgaata gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa
     6901 gtatagcttc tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca
     6961 ccgtttattc gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact
     7021 gaaggagcta tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta
     7081 gtacatttaa aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat
     7141 aatgtttgtt acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat
     7201 tggagtagtt cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc
     7261 atgtctgcat tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt
     7321 gtcacagctt ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt
     7381 gcttcatcta aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat
     7441 tgttgctgtc cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct
     7501 ggcagtgttc gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct
     7561 cttgtggcat ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag
     7621 gtattttgta aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata
     7681 tcaagaccgt cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg
     7741 actaaagtcg tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt
     7801 actgaaaaat cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt
     7861 ttgaaagcta tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt
     7921 tcatctgttt catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc
     7981 gagttgatca cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga
     8041 taagcttgta agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc
     8101 gcacttatga tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc
     8161 ataaattgag cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct
     8221 tctgcatgag cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc
     8281 attcgattat ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg
     8341 ttcgcaatct tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta
     8401 agcttgcttt cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta
     8461 aaaaattgat tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc
     8521 cgactaatga tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat
     8581 ttttttgtga tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc
     8641 cgttcctgaa taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca
     8701 gggaacaaca atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt
     8761 atttctgata agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat
     8821 aggttcccga ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc
     8881 attttccaaa aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc
     8941 cttctttcta tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta
     9001 gttcttcatg agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg
     9061 cttgttggat tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt
     9121 tatgaagggc tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat
     9181 cgaatacata gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct
     9241 ggccccctga ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg
     9301 tgtccactag ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg
     9361 catattcatt tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct
     9421 gagggacata actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac
     9481 caccaagggt tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac
     9541 tactttttcc agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga
     9601 aatcaatatc ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa
     9661 aagcagctgc atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct
     9721 caatttcgat ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag
     9781 ggataatgac caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt
     9841 cgactacagc agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa
     9901 accacaaaat agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag
     9961 caaatattcg gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc
    10021 gcttttcttc atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt
    10081 ctctcaatgt gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc
    10141 ctcgtttcat cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc
    10201 atgttagtaa tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg
    10261 ctggtaagat catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg
    10321 tcattcttgt caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt
    10381 ggaatttacg gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg
    10441 aaaagtaact agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa
    10501 ctccaatttt ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg
    10561 ctactaattt tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa
    10621 ggagagcgag tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc
    10681 ttttttcctg atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa
    10741 gaataaactg aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc
    10801 ggaactattt gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg
    10861 ttcaagtaga caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct
    10921 ttatgatatc gttttttata gaaaattaga tataccgatc aaacagatga agaatttata
    10981 tggaaaaaca ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa
    11041 agaactggtg gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa
    11101 attgatgatc gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat
    11161 gatctcaacg gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag
    11221 ctttgggatg atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga
    11281 ccctagtgaa gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt
    11341 ttatcgtcga ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga
    11401 aatcagagaa agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct
    11461 attgacgaat acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat
    11521 gaaaaaatag gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa
    11581 gaaaaatttt ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata
    11641 gaattttagt tttttagagg aaatatgtct ttaaaaatgg aatggttgca attctta
//
LOCUS       DAYYHS010000088        11642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000088 DAYYHS010000000
VERSION     DAYYHS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.088"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..140)
                     /locus_tag="ACHA6A_002382"
     CDS             complement(21..140)
                     /locus_tag="ACHA6A_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8970545.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            319..1980
                     /locus_tag="ACHA6A_002383"
     CDS             319..1980
                     /locus_tag="ACHA6A_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8970546.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2050..2412)
                     /locus_tag="ACHA6A_002384"
     CDS             complement(2050..2412)
                     /locus_tag="ACHA6A_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8970547.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2480..3421
                     /gene="opp3b"
                     /locus_tag="ACHA6A_002385"
     CDS             2480..3421
                     /gene="opp3b"
                     /locus_tag="ACHA6A_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8970548.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3422..4465
                     /locus_tag="ACHA6A_002386"
     CDS             3422..4465
                     /locus_tag="ACHA6A_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8970549.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4471..5541
                     /locus_tag="ACHA6A_002387"
     CDS             4471..5541
                     /locus_tag="ACHA6A_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970550.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5531..6484
                     /locus_tag="ACHA6A_002388"
     CDS             5531..6484
                     /locus_tag="ACHA6A_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970551.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6496..7506
                     /locus_tag="ACHA6A_002389"
     CDS             6496..7506
                     /locus_tag="ACHA6A_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8970552.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7791..8612)
                     /locus_tag="ACHA6A_002390"
     CDS             complement(7791..8612)
                     /locus_tag="ACHA6A_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8970553.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8709..8987)
                     /locus_tag="ACHA6A_002391"
     CDS             complement(8709..8987)
                     /locus_tag="ACHA6A_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970554.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9255..11264
                     /gene="metG"
                     /locus_tag="ACHA6A_002392"
     CDS             9255..11264
                     /gene="metG"
                     /locus_tag="ACHA6A_002392"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8970555.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
BASE COUNT         3876 a         2106 c         2348 g         3312 t
ORIGIN      
        1 ggcttttgaa caccgtttat tcagataagg gactgtgaca agacttttgt cacagtttct
       61 ttttgtttat tcttttttat ctttgtatgg caataataga caatataacg aaaattattt
      121 aatggctttt ggaagaccat ggaaaacctt gtacaaagct attttttctg aaaaattcct
      181 tgaaacctca cgaaaaaatc attattttat gaaaaagaca aaaaaaagtg ttgatttttt
      241 aataattatt cattaaactc ttaaacaact tcagaaaata ttaattattc tgaaaattcc
      301 aataaagggg tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc
      361 cttgtacttg ctggatgtta tggcggcagt tcagccacaa gcagtagttc agcaaaaagc
      421 ggttcagcag atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct
      481 gacttatcag ttgcaacaga tacaatcagt tttacagcat tgaataacgt ttatgaagga
      541 atttaccgtc ttgatgaaga cagcaaacca cagccggcag gagctagcga gttggcagaa
      601 gtcagtgaag acggattaac ttacaaaatc aaattacgag aagacgcaaa atggtcaaat
      661 ggtgatcctg ttacagcagc ggattacgtt tatggctggc aacgaacagt agacccagct
      721 acagcttctg aatatgctta cctgtatgcg ccagttgaaa atgcagaaga cattacagct
      781 ggaaagaaag ataaatcaga actagggatc aaagcagttg gtgactatga attagaaatc
      841 aaattgacga aacaaacacc atatttccaa tatctgcttg ctttcccatc atttttccca
      901 caaaaacaat ccgtagtgga agaaaatggg gatgcctatg catctgcaag tgataaagct
      961 gtttataatg gaccattcac attagaagat tttgatggac caggaacaga tacagaatgg
     1021 acttataaga aaaatgatca ttactgggac aaagatacag taaaactttc tgaaatcaaa
     1081 gtcagtgtag taaaagaatc ttctactgca ttgaacttat tcaaagatgg ccaagcagat
     1141 gacgtgatct tgtctggtga gttagcacag caaaacgcaa acgatccagc ttatacttct
     1201 gttaaagaag cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc
     1261 aacaatgtga acctaagaaa agctatttct tattctatcg atcgtgaagc tttagtaaaa
     1321 caagtactag gtgacggatc agttgtctct acaggattga tcccatcaga tatggtaaaa
     1381 agtccaacaa cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa
     1441 gataaagcaa aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa
     1501 tttgatttga tggcttctga tgatgattca tcgaaaaaag taatcgaata tgttcaaaac
     1561 tcaatccaag aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc attctctgta
     1621 cgtcttgacc gctcaacttc gggtgatttt gacgcagtac taggcggatg ggctgctgac
     1681 tatgcagacc caagcagctt tactgatttg tttgtcacag gtaattcata caaccgtggt
     1741 cgctggagca atgcagaata tgacgaagca gttaaagcag cttcatcaac agacgctggc
     1801 gatgaacaag cccgttggga tgatttccaa aaagcaatca agatcatctc tgatgatatg
     1861 ggtgtcgttc cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt
     1921 atcgttcacc atgcggcagg cgcttcatgg gattacaaat ggacatacgt tgaagaataa
     1981 tcgttaatac cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca acctcgttct
     2041 catattcact tatcctaaca aagaaaaaag catgaaaata gcagaaagag caattagtat
     2101 tccggcaatg atcagccaaa agctgtacat accgtagctg gcagacgaga gcgtggaaaa
     2161 ttctggtttt ttctttcggt tccttgttcg tgcaagatgg accgatcgct gaaaatgatc
     2221 gaagaaaccc gaagaaaaga cccataaaaa accgccgata atcagaaacg gcaatgacca
     2281 taagaataga tcattagata attgaatgag tgtaaaggaa tggttgatga gattttttat
     2341 taaaataacc agaatcagta gaaaagctac tgcgtagggg atagattttt tttcatttga
     2401 ttgctccttt atttttggtt cattatctaa taatgtagcg aaacatctgt cagaagtcaa
     2461 aagctagggg gaaacattag tgaatagtta tatcaaatat gtattaaaac gtgtcttttt
     2521 catgatcatc acgctttggt taattgcaac aatcaccttt ttcttgatgc aattgctgcc
     2581 gggcacgcca tatacgaatc aggaacgttt gagtcctgaa acaatcgaga tgctaaataa
     2641 acaagtcggt ttggataaac cagttatcgt acaatatggg atttatcttt ctaatttgct
     2701 tcaaggagat tttggaattt ccttccaatt taaaaaccag ccagtcgcac atttgttagc
     2761 tggaagaatc ggaccatcct tgcagttagg gttacaggca attatctttg gaacagtcct
     2821 agggacgatt ttaggaacga tctctgccat gaagcaaaat acatgggcag atacatcttc
     2881 tacactcgta gcgatattag gtcgttccat tccaaacttt gtatttgctg ttttattaca
     2941 atatatcttt gcaatcaaat tacaagtttt gccaatcgca aaatgggatg gctttgtgta
     3001 cacaatctta ccaacaattg cgttggccat gtcgccgctt gctgactctg ctcggtttat
     3061 ccgtacggaa atggtggaag tactgcatag tgattatgtg gaattagcaa aagcaaaagg
     3121 attgagccgt tgggaaatcg cctttaaaca tggtcttcga aatagtttga ttccattgat
     3181 gactttatta ggaccgttag ctgtagcatt gatgaccgga tcactggtgg tagaaaatat
     3241 ctttgctatt cctggtatcg gggaacaatt cgttaagtca atcacaacaa atgattatcc
     3301 tacgatcatg gctgtaacga tcctttactc atttatgctg atttttgtta ttttgatcgt
     3361 ggatctattg tacggtctag ttgatccaag aattcgtgtt tcagaaggga gccgaggata
     3421 aatggaaatg ataccaaagc gttatgaaac agttgcagat atccctgcgg atgaatttct
     3481 tccattacaa aaaaataccg aagaagaacg tgaacagatt actgcacctt ctttgaattt
     3541 tatccaagat tcatggcgcc gtttaaagaa aaacaaagct gccgttattt caatggtttt
     3601 actattggtc atcattttga tctctgtgat cacaatcttt gtttctcctc atgatccaac
     3661 tgcccaaaac gtggcttata tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg
     3721 attgaatgga aaagcaatgg ttggtggtga acttgttgat aaatatgctc aagcaaacgt
     3781 cccttcaaac gtaaatttct ttttaggaac agacggtttg ggacgtgatg tattaagccg
     3841 cttatttatg ggaatgagaa tctcgttgct gatcgcattt attgctgcat tactagatgt
     3901 aacgattggt gtagcttacg ggttggtttc tggtttgtta ggcggacgtg tcgataacgt
     3961 gatgcaacgt tttttggaaa tcttatcagg aattccaaac ctcgttgtca tgattttgat
     4021 gttggtcgtt tttgaaccag gaattttttc aatcgtggct gctatggcca tcacaaactg
     4081 gataccaatg gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt
     4141 tttagctgga ttgacattag gtgaatcaag aatgaaaatt gcattcaaac atattttgcc
     4201 gaatatctct agtgtcatta ttattcaaat gatgtttagt attccatctg ctattttctt
     4261 cgaagccttt ttaagcttta tcggtttagg tcttacgcct ccgtcagctt cattaggaac
     4321 gatgttgagc gaagggtaca agacattcct gtatctgcct tatctattat ggattccggc
     4381 agtaacttta tcggttatca tgattggctt taacttactt gctgatggat tgcgtgatgc
     4441 ctttgatcct aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt gaaagactta
     4501 gaaatctctt ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt caattttcat
     4561 ttgaataaag gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg
     4621 accagaagca tcatgcgctt gctgtcaagc aacgccaata tagatgctgg tcaaatcctc
     4681 tttaaaggta acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa
     4741 gaaatagcga tgattttcca agatccaatg acctctttag atccgacgat gcccattggg
     4801 aagcaagtcg ctgagtccct tagaaagcat aataaagttt caaaaaaaga gggacaaaaa
     4861 gctgcattag aattgcttaa attagttgga atacccgaag ctgagaaacg tatcaacagt
     4921 tatccgcatc agttctcagg aggtcagcgt caacggatcg tgatcgcgat tgcactggta
     4981 tgttatccgg aaatcctgat tgctgatgaa cctacaacag cgcttgatgt gactattcaa
     5041 gcacagattt tggagctgct gaaagaaatt cagacaaaaa tcgatacatc aattatcttt
     5101 atcactcatg accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga
     5161 ggaagaatcg ttgaagttgg aacatcggaa gaaattttct acaacccgca acatccctat
     5221 acttggggac tgcttggttc aatgccgaca ttagatagtg cgaacgatcg attgtatgct
     5281 attcctggtt ctccaccgga tttattgaac ccgccaaaag gagacgcatt ctatccaaga
     5341 aatgaattcg cgatgaaaat cgatgcagaa caagaaccac cattttttga actatcaaaa
     5401 acacacaagg cagcaacttg gctacttgct ccgcaggcac ccaaagtgac accgccagaa
     5461 gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa aacatactta cggtcaagga
     5521 ggaattgctg atgccaaata aactacttga agttaaagga ttgaaacagt atttcaatgt
     5581 aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc tttcacattt atgaaggaga
     5641 aacattcggt ttagttggag aatctggaag cggaaaatcc actactggcc gtacgatcat
     5701 tcgcttgaat gaacctactg atggtgagat attattcgac ggtcaagata taacgaagat
     5761 caaagataaa aaaggtttga cgaaattccg gcatgatgtt cagatgatct ttcaagatcc
     5821 atacgcttcg ttgaatcctc gtatgaaagt cagagatatc attgccgaag gaattgatgt
     5881 taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac
     5941 tgttggattg aatccaagcc acggtactcg ttatcctcat gaattttcag gtgggcaacg
     6001 ccagcgtatt ggtattgcac gagcattggc agtaaaaccg cgttttatca tttgtgatga
     6061 accaatttct gcactagatg tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct
     6121 ccaaaaagaa catcaattga cctatctatt tattgcacat gatttgtcga tggttaaaca
     6181 tattagtgac cgaatcggtg taatgcataa cggcttgtta ttagagatgg gaacaagtga
     6241 tgaaatctat aatcacggtg ttcatcctta tacagaaagt ttgttatctg ctattcctct
     6301 accagatcca gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg aaccagatga
     6361 cggacaagta cgtcaacttc gtgaaatcgc accagaacat tttgtctatg caactgagca
     6421 ggaagtccca tattacgcaa aaaaactaaa acgacaaaag gaatctcttc tggttgctaa
     6481 ctaggaggga catagatgct aaaagctttt aaattccgta tgtatccgac agaagaacaa
     6541 aaacagcaat tgattcgtac ttttggatgt gctcgattta cctataacca tttgctgaaa
     6601 aagcggcaga aaagttggca acaaacagga gtagccaatt tttcgctcac acccgctaca
     6661 ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc ttgctttagc aaatgcacag
     6721 cttaatttgg atcgggcttt tcgcaattat ttcaagggac gtgccagctt cccaaagctt
     6781 aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca accaaacccg tacgatctat
     6841 ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac taatcaagat aaatcagcat
     6901 cggccagttg aaggatcgat tcgctccgct acgatttctg ctcgatataa tgaagaattt
     6961 tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag aatctttagc taaatggatc
     7021 gggatcgcct atcatccgaa gacattgatc caaacatctc gacctctaga agtaacttta
     7081 ccaaaatttc accagacaga agaaaagctg caacatgcac agcggaaatt gaatgtgaaa
     7141 gtccgctccg ctcaccatag aaagatcagg ttagatcagg caagcaatta tcagaaacaa
     7201 aagagaaaag taatggatct ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa
     7261 caattatctg ggaaattggt caaacaatat gactacttat ttgtagaatc ttttccaaaa
     7321 gaagaggcac atgctgattt ttctattcac gactggcata aattaataac aaaattgcgt
     7381 tataagtccc aatggtataa taaaaaattt cttttgatca ataccgacgg agcagaagaa
     7441 agcaattccg taagaaagag ccaagtagta gaaaaaatgg gcagacattc ggttatcaag
     7501 ggataaaaaa tgaatgaaac tgataggagc gttagggtta gccttgttaa taaaaagtct
     7561 taatgacttt tgtttctagg aatttttctt ttgaagaaaa gctcactaat tatgacaaac
     7621 caataaaaca atagcagaaa aagttcgtac gagctttttc tgctattgtt aattttttat
     7681 tcagaaagtt tacgaataca tcaaaattct gaaacaaaaa acgtaactca caaagagttg
     7741 tttttgcgtt ttttcttgaa tgattaggtc tgaatctttt gctcacaacc ttattcatgg
     7801 tgccccggct tgagctgata gatttttgaa ggacggccga ttcctacagg acgttcaccg
     7861 atctcatcga aatattggag catagctttc ttaaaattgg aatgatcgat cgttttataa
     7921 tcgatcccta agaatttagc aaatactttt cgagcttccg taatcgtgaa atctttacct
     7981 aaaacttgta gaacttgcgg ctcgtgttcc attttattga ctacacggtt gaatgctttc
     8041 aagatgattt gactatggtc aaaagccaaa gtatctttgc cagaagactc tccagtgact
     8101 aaatcaagcg agatagccac ttctccgcta gataaatgga tcttgttact atgacgttct
     8161 agtgtgaacc agcgtacttc cttggcatca tctccggcga ttagcggttc ttctccaata
     8221 aaggctaagt agctgactgt gacgacccag cctcgcggat ctcgattagg cgtactgaat
     8281 gtatgcagtt gctcaatatt ttcttttgaa ataaccacac cagtttcttc ctttgtttct
     8341 cttaagacgc tttctccagt tgactcatcc ttttggacaa agccaccagg caatgcccat
     8401 gaatttcgat aaggatgccc ttttctttgg atcaacaaaa ctttcagctg atcttcttct
     8461 ttgttatagc acatcagtac gatatcgaca gtcaaagagg gcttgtcata ttctggacgt
     8521 tcctgttttt gataccagtt aagaaattct tcttcacttg ctttatgttc ataatataat
     8581 ttttcttctt ctttagaagc aaaacggttc atctgaccac ctccattgac agtaaaagta
     8641 ctttttacct tattgtagca gactctgaaa aaaaaccaag gatttacttg aaaaatctag
     8701 gttatcattt aaatatgaaa aataaatgga agtatgagat tgaataaaaa gaaaagcaga
     8761 taaaacggca atactttata ccgctcttca cgaatggcaa agaagccaca aataacgata
     8821 tatacgtcaa tgataaaacg caaaatccaa gctgctgtca taaaactgtt caaatgccca
     8881 tggaggttaa aataaccata catacgaata taaaaatagc tgaatattaa atgtgctaat
     8941 gaaagaaaag caatactaag tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc
     9001 ccttactaag atagatagtc tttattatac cacgtgtgag gggagaaata aaaagccgtt
     9061 ttttagtaga tatgaaacaa aacaaaaacc cgcatttatt caacctattc atccttttaa
     9121 aggatagatt gaacgtagat agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc
     9181 taacagcatg ctttttttat ggtatactag caaggaaacg aattcgcatc atgcgataaa
     9241 ggaggaagag ccccgtggca gaaaaggaaa ctttttatat cactacgcca atctattatc
     9301 caagcggcaa acttcatatc ggcaactcgt atactacgat tgcatgtgac gcaattgctc
     9361 gttataaacg gttgatgggc tttgatgtgt tttatctcac tggagtggat gaacatggtc
     9421 aaaaaatcga aacaaaagca gaagaactag gtgtaaaacc gcaagaatat gtagataaaa
     9481 tggcggctga tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat aaattcattc
     9541 gcacgacaga tgattaccat aaagcagctg ttcaaaaaat atttgatcgt ttattagaac
     9601 agggagatat ttacttaggt gaatatgaag gctggtactc tgtttctgac gaagaattct
     9661 ttacagaaac acaacttgca gaagtgtaca aagatgacga aggtaacgtg atcggtggta
     9721 aagcgccaag tggacacgaa gtagaattgg taaaagaaga atcatacttc ttccgcatga
     9781 gcaaatacgc agaccgtcta ttggaatact atgaagaaca tcccgaattc atccagccag
     9841 aatctcgtaa aaacgaaatg atcaacaact ttatcaaacc aggattagag gatcttgcag
     9901 tttcacgaac aacttttact tggggagtac cagtcaagag tgatccgaaa catgtggtgt
     9961 atgtatggat cgatgcatta tccaattata ttactgcact aggatatggc tccgaagatg
    10021 aaagtctatt cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc
    10081 gctttcatac gatctattgg ccaatcatgc tgatggccct tgatttgcct ctacctaaaa
    10141 aagtatttgg gcatggctgg ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa
    10201 atgtcgttta tcctgaaatg cttgtggaac gttatggttt agatgcactc cgttattatt
    10261 tgcttcgtgc tgttccgttt gggtcagatg gtgtctttac gccagaagat tttgtttccc
    10321 gtgtcaatta tgatctggca aatgatctag ggaacttatt gaatcgaacg attgctatga
    10381 tcaacaaata ttgtgatgga ctcgttcctg actatgcctc acttgtcact ccatttgata
    10441 gtgaactgtc tacgacggct gctaacgttg tcggacgtta ccatgatgca atggaaaaaa
    10501 tggaattcaa tacagcattg gctgaaatct gggtgctgat ttctcgtgca aacaaataca
    10561 ttgatgaaac agaaccatgg gtattagcaa aagacgaaga aaagaaagct gaattgaata
    10621 gtgtcatgat ccatttagct gaatctttgc gtatcgcagc gatattactt cagccaatca
    10681 tgacagaaac gccagagaag atcttcaatc aactaggatt ggactctgaa acaatgaatc
    10741 ttgaaggatt acactttggc gaatttcctt caggaacaaa agtagttgca aaaggtacac
    10801 caatcttccc acgtttagat atggaagaag aagttgcttt tattcaagaa aaaatgtctg
    10861 aaggtactca aacaaacgaa gatacagtca aatgggatcc agaagaaaca gaacttgttt
    10921 caacgaaaga aaaacaaatc aaatttgatg tatttgaaaa agtagaacta aaagtagcag
    10981 aagtcatcaa ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc cgactagatg
    11041 ctggagacag ccaagaccgt caaatcttgt caggaatcgc tgaattttat cctgatccaa
    11101 gcgaattgat cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg
    11161 gacaaatcag tcaaggaatg atcctttctg cagaagcacc agatggctct ttacaagtaa
    11221 tcgaagcacc aaaatccatg ccaaatggct cagaaatcgc atagaaattc aaagggggta
    11281 tgacactagt tgtgtcatac cccctttttc cgaaatttga gaagcaattt tcgtaaattc
    11341 cttcttatta cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc
    11401 ctagaaaaag aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa
    11461 tcgtaaaaac agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc
    11521 tggtactctt ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat
    11581 ctatatttat atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt
    11641 ca
//
LOCUS       DAYYHS010000089        11469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000089 DAYYHS010000000
VERSION     DAYYHS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.089"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..1883)
                     /locus_tag="ACHA6A_002393"
     CDS             complement(117..1883)
                     /locus_tag="ACHA6A_002393"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970556.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1843..3597)
                     /locus_tag="ACHA6A_002394"
     CDS             complement(1843..3597)
                     /locus_tag="ACHA6A_002394"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970557.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3707..4276)
                     /locus_tag="ACHA6A_002395"
     CDS             complement(3707..4276)
                     /locus_tag="ACHA6A_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8970558.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4294..5709)
                     /locus_tag="ACHA6A_002396"
     CDS             complement(4294..5709)
                     /locus_tag="ACHA6A_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970559.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5720..6388)
                     /locus_tag="ACHA6A_002397"
     CDS             complement(5720..6388)
                     /locus_tag="ACHA6A_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8970560.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6518..7102)
                     /locus_tag="ACHA6A_002398"
     CDS             complement(6518..7102)
                     /locus_tag="ACHA6A_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8970561.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7433..7666)
                     /locus_tag="ACHA6A_002399"
     CDS             complement(7433..7666)
                     /locus_tag="ACHA6A_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8970562.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7681..8631)
                     /locus_tag="ACHA6A_002400"
     CDS             complement(7681..8631)
                     /locus_tag="ACHA6A_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8970563.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8628..9470)
                     /locus_tag="ACHA6A_002401"
     CDS             complement(8628..9470)
                     /locus_tag="ACHA6A_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8970564.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9472..10191)
                     /locus_tag="ACHA6A_002402"
     CDS             complement(9472..10191)
                     /locus_tag="ACHA6A_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8970565.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10739..11041
                     /locus_tag="ACHA6A_002403"
     CDS             10739..11041
                     /locus_tag="ACHA6A_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970566.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            11113..>11469
                     /locus_tag="ACHA6A_002404"
     CDS             11113..>11469
                     /locus_tag="ACHA6A_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8970567.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEY"
BASE COUNT         3920 a         2056 c         1524 g         3969 t
ORIGIN      
        1 atcatactga ttctttcgtg tattcaaaat tagatgctgt ttaataaaat tattcttttt
       61 gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct atttcatcat
      121 cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga
      181 tttttgtata tttctgacct tcgatacaac tcattatgag taccttgatc aactagtttt
      241 ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata
      301 attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt
      361 tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa
      421 gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg
      481 accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga
      541 gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa
      601 atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa
      661 gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca
      721 tacagtctag acactaattt caagatacta gttttcccgg aaccactagc acctactaac
      781 gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta
      841 tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat
      901 ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa
      961 aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct
     1021 agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct
     1081 actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa
     1141 aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt
     1201 ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt
     1261 tcttgaaatt cttccgagtt ctctctcaaa gtatcgtaat atttagtatt ggctttcgtt
     1321 tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct
     1381 aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc
     1441 ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac
     1501 atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt
     1561 gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt ctttataagt actattatat
     1621 aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac aaacgatacg
     1681 gcaaaataaa gccaacgggg ctttgtattt tctaaaacta attcgtctat cagcatcatt
     1741 ataatgaatg ctggtaccat attaatgcag tatacaaaaa aactagaaat agaagcttta
     1801 gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa acttttcatt
     1861 gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat acacttgatg
     1921 tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag
     1981 aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc
     2041 tttcatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc
     2101 actggcttca tccataataa caatctttgc atcttttaaa attgctctgg caatagctat
     2161 acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct
     2221 ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt
     2281 acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag
     2341 tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt
     2401 taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag
     2461 tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg
     2521 ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt
     2581 ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg
     2641 cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg
     2701 catacttaca tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa
     2761 catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat
     2821 aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt
     2881 tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa
     2941 ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc cgcggatata
     3001 ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt
     3061 attctctgtc attttcttta acaagaatac accaattact aacatggcga ttagaaccaa
     3121 tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc
     3181 ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc
     3241 aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt
     3301 taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga
     3361 aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac
     3421 cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa gaaatttgta
     3481 aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata aagtggctaa
     3541 ccatccgtag tatttcattt tagggacata actaaataat tttttgtata ctttcatttt
     3601 ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg ctttccgtaa
     3661 tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat tttttgtatt
     3721 tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct acaactaaca
     3781 ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt gttgcagcag
     3841 attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat ataggaatcc
     3901 actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga ggaattgctc
     3961 gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta atcgaactga
     4021 taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa acaattgtgt
     4081 aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat atgattccag
     4141 ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca atactactaa
     4201 ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca ataaaaatat
     4261 aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat taaaaatctg
     4321 ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc caccattttt
     4381 taaataaata attctattgc atgttttata aagaaattcc ggatcatgag tagctataac
     4441 tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca tattaatata
     4501 atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca atgaagctat
     4561 agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt gatctttcat
     4621 tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa ctatttcatt
     4681 ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca tttcttgcat
     4741 tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt tggtagggcg
     4801 aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt tccctaccaa
     4861 accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat tttcataagt
     4921 aatttcttca acttttaata catctgcact taccttttcg atatagggaa cttcaatcga
     4981 actattaaaa atgtcaaatt tccttaatgc gttattgttt tcagttttta acaactgaat
     5041 agttgtagtt tttacagttc tatttttaac aaatagaact tgatcaatta attctttcaa
     5101 atagtaaaaa cgatgttcag ctataacaat agtaaatcct ttatttttta aatcacaaag
     5161 taagtcagca agtttcttag tcgctttgaa gtccaaattt gaagacggtt catccaatat
     5221 aattaattta gagtttagcg ataaactaca tccaatagaa attttctgcc gttcaccact
     5281 tgatagcgaa aaaatgtttt tatctaatat atttgaaaga tctaataatt cagctaattc
     5341 attgattctt ttatccatat gctcttttgt aaatccaaag ttttccatcg aaaatactaa
     5401 ctcactcgtt gtatttgttg taaagaattg tcctctagga tcttgactta cgtagccaat
     5461 attttttgat atactttctt gtgttaagga ttgtagcgag ttattttcaa ataaaatttc
     5521 cccaaccaat tttccgtcat aaagtcttgg aataagtcca ttcattaact ttaatagtgt
     5581 ggatttccca ctcccagaag aaccagtaat caatgtaaga ctgccttttt ttattgaaaa
     5641 attaaggtca acaagcgctt gttccttaac atccttatat gaaaaagaaa cattgttaaa
     5701 ttcaaacaat tctttcacct caaatcaaaa tacttaagaa aaaaatagta acggaaaaca
     5761 ggatgatata gtctaataag ctcatgtttg ttttatagta agaacttctt ttcacaggag
     5821 attctatccc ctttgttatt gcattagcgg tcatttcttc agcaatcata gttgcacgta
     5881 taagaacggg aactaacaga aattccaaat attgcagagg ctgaaaaaaa gacaagcgaa
     5941 tgtttctcat ttttaaagat tctttaatca ttcttatttc agaacgataa gtcggaaaga
     6001 atctgatcac tactgtaata cttaacatta tttttttcgg taaatgtatt ttttctaaag
     6061 aggataataa ttcattgcta gtatagacac taaccaacaa ccatgagatc attacgaccg
     6121 gcaacattcg aaatacaata tataaggatg tcgcaaaaaa tatagtaaaa aactctatct
     6181 tgatagcatc aataattttc attactaaaa acagaactga ataaatcaag atgaaataaa
     6241 tacatgtctt tattttacga aacatcaata ctaaaactgt agcaataatg aaacacccca
     6301 cttgaaatat atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata tttaaagtaa
     6361 tcaaggttaa cggattgatt tttgacactc tttcacctcc aaaaaataag atcgcactgt
     6421 tatcttaagc tgataaaaat gcaccgttat tcagataaca gtgcgatctt atcaaaaagc
     6481 ataactgaga acaattctca atgcaagctt tttttaatta actcagtaat tcaatccacc
     6541 caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct tttgaatagc
     6601 tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag tggatttctt
     6661 cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta tctagacaac
     6721 agactgtctt tttcgtcaca aatgatgtaa ttttttcaac aatatcttga tatttggttc
     6781 cagctgattg gaataaaatc aaatcgaata tacgtttatt ctcaaaaata taattaataa
     6841 atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata ttttctaagt
     6901 ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta atatgcggct
     6961 gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa gcacccgtag
     7021 ttacgcctgc ggtcaggcac agttgcctta tcttagtttg ctgaaatcct tctttttcaa
     7081 aatggcttag tccactttct attaactttt ggtgagttat aatataatct ctggtttcta
     7141 tgggtacttc ctcctaataa gatgaattaa ctttattatc ttacacagga cttgaagttt
     7201 caatcgttaa cgtttattac tgaactcatt ccttagagct aaactaatga gaaagagcag
     7261 aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa tcattcacct
     7321 tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag gataagtcct
     7381 atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc ttttacttaa
     7441 aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga aatcctatat
     7501 ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct ggactaacac
     7561 ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat aattcctgaa
     7621 ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct accctccaaa
     7681 ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg catagtaact
     7741 atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat agataggaat
     7801 attggtatct tttgatactg ttttcattgg tctatcatcc acactgcttt ctacgaataa
     7861 ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat ctggtgttcc
     7921 ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg ctttcgagaa
     7981 atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa ttgaagcaaa
     8041 tttagattta gcttctttgt ctagagaatc caatttttca atatacttat ctagattttc
     8101 tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa tatttttagc
     8161 gtaaataata ccgttttcta aatttaacca agcatgagga tcttccttcc ctttctcact
     8221 ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt ctttgttttc
     8281 ctctttgttc gcatttttaa ctaattttgt aaaccaagca tttcctccag tttccaagtt
     8341 aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt caggcaaagg
     8401 ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa ttttatcttt
     8461 tgctatattt tcggtaatat ctgctaaaat cgaattagta actactactt ttaatttttc
     8521 gttcgaagcc actttatctt tgtctttact atttattgaa catgccccga atataaaaag
     8581 agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca taacgatctc
     8641 tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac aaataagaat
     8701 gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc aataaacaat
     8761 ccaagcaatg atgaacttgc tccgactgtt gcagaaataa atatcatagt tttcaagcta
     8821 tttgcatata aatatgctgt agctgctggt gtaattaaca ttgcaacaat taatatagtt
     8881 cccacacttt gcatagctgt tactgctact aaagttaata gaaccatcaa tagataatga
     8941 taaaaactga cattcatccc tattgctttt gccattaatg gatcaaaaga agtgattaat
     9001 aattctttaa aaaatagaac tactataacc aacactaaaa ccgaaacgcc aattgtaatc
     9061 cacttatcaa tatcttgtac agctaaaata tttccaaata aaatatgaaa taaatccgtt
     9121 gagctatttg caactcctat aaggataact cctagtgcta aaaaagaact gaaagtaata
     9181 ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat aatgatagaa
     9241 gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat ataagaaatt
     9301 gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat tcctcttaaa
     9361 ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt aattagagca
     9421 ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat attattcccc
     9481 ctctccaata aatatagtat ctccataggc tttttttaaa ttttcttcat taaatacaga
     9541 ttctacactt ccgtgagcaa ttagatttcg attcaaaaga ataatattat caaaatactt
     9601 ctttaccttg cttaaatcat gatgcacaat tagcactgtt tttccctgat gtttcaattt
     9661 ttttaacgta tccataatta ttcgttcact aactaagtct atccctacaa atggttcatc
     9721 taaaaatatg aaatccgcat tttgagcaag acatcttgct aaaagtacac gttgaaattg
     9781 tcctccagat aattctccta tttgatgatt agaatatttt cccatatcga ccttttccaa
     9841 agcctttgaa actctttgcc attctgcatt tttaattctt tgaaaaactt tcattccggc
     9901 atatgttccc atagatacac attcttttac ttttattgga aaagtaaaat caatatcagc
     9961 tttttgctct acatatgcaa tcttcaccag ttttttattg atatcttcat tattaatgga
    10021 gacctctccc tgatgaggga taatatttaa cattgcttta agcaatgtgg attttccagc
    10081 accatttgga ccaataatac ctgttaaagc accttcttgt atttttaaag aaatctcgtt
    10141 caatgctaaa aaatcgttat aacaaacatt aagctttttc acttctaaca taactttctc
    10201 cttctaaatg ataatgatta tcacgatcat atggtataca aaaattatat tttagtcaac
    10261 cctaaatttt atagtttaga tttttttctg ttgctgaaga ttagaatgac aaaactattt
    10321 gaaactacgc gtatagtaag tagctttcaa aatatataaa aaaggagcgt tctcaaatga
    10381 gaacatgctc ctaaaactac tttattggcg tatggaaaaa ttatacttga agtggatagc
    10441 gggttgaata agttgataag catcaaaaca tttataaaaa ttattcaaga ctatgtctgt
    10501 tccatataca aatatgaaaa tcagtaaact ccatttaact tttttcgtag tgtgtctttt
    10561 tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa ctatttttga tcattgacaa
    10621 ttaaatacaa tttttgaata acgatccctg ctattcgagc tagaaactca agtacgccat
    10681 gacctgatta gcgacaaata cttagagatt tctttttgtt ctgggaaaac aaaatgccat
    10741 gacaatagca gcaaataatg atacttccgt aaggctcgcc gtaaactggg gagaggtgaa
    10801 attcctatgt gcttacccaa agcaattcaa aaatttcata actactattt taggagatga
    10861 ttttatagaa tccagagtct acctgaataa aaaacaaacc tgtgcatttc taggaatcga
    10921 taataatact ctagataagt ggattcgtat atataatttc ccagtaataa gagttggtgg
    10981 tgtagttcgc tttgacgtag aagatattaa agactttatg aatacattta aaataacttg
    11041 aaattttctt ctcgccgcat atactaaaat tagtgaaata tactgcctta ctttttttaa
    11101 ggagtgagga ttttgcctac aaagctttca aatgggaaat ataaaactaa tctgagatat
    11161 ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa aacatttcca
    11221 aataggcaat tggcgattaa agccgaaaat gatatgaaga agaaaataga aaaagttttg
    11281 agagaggaga atgcaaactc attagaatta aaaggaaaaa taacgtttaa aaaattttac
    11341 gaatctaaat ggcttcctag atatgaatta ggtcaaacaa ttagatcaaa caggcctcct
    11401 agtgatataa ccattagtaa tacaaaagat atatttcggt tgcatatttt acctatgttt
    11461 ggtgaatac
//
LOCUS       DAYYHS010000090        11267 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000090 DAYYHS010000000
VERSION     DAYYHS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.090"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHA6A_002405"
     tRNA            12..84
                     /locus_tag="ACHA6A_002405"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHA6A_002406"
     tRNA            88..160
                     /locus_tag="ACHA6A_002406"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHA6A_002407"
     tRNA            168..249
                     /locus_tag="ACHA6A_002407"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHA6A_002408"
     tRNA            267..339
                     /locus_tag="ACHA6A_002408"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHA6A_002409"
     tRNA            351..422
                     /locus_tag="ACHA6A_002409"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHA6A_002410"
     tRNA            439..524
                     /locus_tag="ACHA6A_002410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHA6A_002411"
     tRNA            535..608
                     /locus_tag="ACHA6A_002411"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHA6A_002412"
     tRNA            618..691
                     /locus_tag="ACHA6A_002412"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHA6A_002413"
     tRNA            711..783
                     /locus_tag="ACHA6A_002413"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHA6A_002414"
     tRNA            811..884
                     /locus_tag="ACHA6A_002414"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHA6A_002415"
     tRNA            898..971
                     /locus_tag="ACHA6A_002415"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHA6A_002416"
     tRNA            995..1084
                     /locus_tag="ACHA6A_002416"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHA6A_002417"
     tRNA            1097..1170
                     /locus_tag="ACHA6A_002417"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHA6A_002418"
     tRNA            1189..1264
                     /locus_tag="ACHA6A_002418"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHA6A_002419"
     tRNA            1266..1336
                     /locus_tag="ACHA6A_002419"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHA6A_002420"
     tRNA            1350..1423
                     /locus_tag="ACHA6A_002420"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHA6A_002421"
     tRNA            1448..1535
                     /locus_tag="ACHA6A_002421"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHA6A_002422"
     tRNA            1557..1628
                     /locus_tag="ACHA6A_002422"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHA6A_002423"
     tRNA            1651..1740
                     /locus_tag="ACHA6A_002423"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHA6A_002424"
     tRNA            1753..1826
                     /locus_tag="ACHA6A_002424"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHA6A_002425"
     tRNA            1845..1917
                     /locus_tag="ACHA6A_002425"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHA6A_002426"
     tRNA            1921..1993
                     /locus_tag="ACHA6A_002426"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHA6A_002427"
     tRNA            1998..2068
                     /locus_tag="ACHA6A_002427"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHA6A_002428"
     tRNA            2081..2154
                     /locus_tag="ACHA6A_002428"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHA6A_002429"
     tRNA            2166..2237
                     /locus_tag="ACHA6A_002429"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHA6A_002430"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHA6A_002430"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8970568.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHA6A_002431"
     CDS             3826..5298
                     /locus_tag="ACHA6A_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8970569.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHA6A_002432"
     CDS             5381..6169
                     /locus_tag="ACHA6A_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8970570.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHA6A_002433"
     CDS             complement(6236..6904)
                     /locus_tag="ACHA6A_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8970571.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHA6A_002434"
     CDS             7019..8731
                     /locus_tag="ACHA6A_002434"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970572.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACHA6A_002435"
     CDS             8734..10506
                     /locus_tag="ACHA6A_002435"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970573.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11267)
                     /locus_tag="ACHA6A_002436"
     CDS             complement(10588..>11267)
                     /locus_tag="ACHA6A_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8970574.1"
                     /translation="YGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQK
                     SSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTG
                     SYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKEL
                     NLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVE
                     KLGKIEI"
BASE COUNT         3241 a         2018 c         2548 g         3460 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggttcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa gaaaccccat ttacgggatt atattttaat ccatatc
//
LOCUS       DAYYHS010000091        11219 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000091 DAYYHS010000000
VERSION     DAYYHS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.091"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..885
                     /locus_tag="ACHA6A_002437"
     CDS             37..885
                     /locus_tag="ACHA6A_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8970575.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1531..1737
                     /locus_tag="ACHA6A_002438"
     CDS             1531..1737
                     /locus_tag="ACHA6A_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8970576.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1939..2940)
                     /locus_tag="ACHA6A_002439"
     CDS             complement(1939..2940)
                     /locus_tag="ACHA6A_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8970577.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2945..4858)
                     /locus_tag="ACHA6A_002440"
     CDS             complement(2945..4858)
                     /locus_tag="ACHA6A_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8970578.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5026..5532)
                     /locus_tag="ACHA6A_002441"
     CDS             complement(5026..5532)
                     /locus_tag="ACHA6A_002441"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8970579.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5692..6132
                     /locus_tag="ACHA6A_002442"
     CDS             5692..6132
                     /locus_tag="ACHA6A_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8970580.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6158..7315)
                     /locus_tag="ACHA6A_002443"
     CDS             complement(6158..7315)
                     /locus_tag="ACHA6A_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970581.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7318..7674)
                     /locus_tag="ACHA6A_002444"
     CDS             complement(7318..7674)
                     /locus_tag="ACHA6A_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970582.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7972..8946)
                     /gene="sgrA"
                     /locus_tag="ACHA6A_002445"
     CDS             complement(7972..8946)
                     /gene="sgrA"
                     /locus_tag="ACHA6A_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8970583.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9142..9981)
                     /locus_tag="ACHA6A_002446"
     CDS             complement(9142..9981)
                     /locus_tag="ACHA6A_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8970584.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10166..11053
                     /locus_tag="ACHA6A_002447"
     CDS             10166..11053
                     /locus_tag="ACHA6A_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8970585.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         3428 a         2198 c         1709 g         3884 t
ORIGIN      
        1 aaaatgataa tcttattatg aaaatgagga attctcatga gcgtctccat aagactacaa
       61 atcaaaacaa tgtacaacga cttcagccca aaagaacagg ctatcgccga ttatatttta
      121 gaaaatccaa gcaaagtaag tcatagtcct attagcaatt tagcaagtga actgggcatt
      181 gctgactcta ctttttttca atttacaaaa aaacttggtt ataatggatt taaagatttt
      241 aaaatggcca tgttgatcca agaaaatgat ctatctgcta tttctatcca cgaaaatatt
      301 cagaaaacag atactgagct cactatggcg caaaaggtgt tcgagtctag tatttcaact
      361 ttaaatgaca caaaaaagtt gttacaacaa aaggatctaa aaaaagcggc tactattatc
      421 aatgaatcta aacgtgtcta ttttttcggt gttggcggtt cagagattgt agcaactgac
      481 gcttatcata aatttttgcg ttcccctatc gcaacttctc acagcacgga ttaccatatt
      541 caattgatgg aagcttcttt attgacggaa aaagactgtg ctgttttgat ttctcacaca
      601 gggcaatcaa aagaaacaat ccatattgct gaaaccgtaa gaaaaactgg agcaaaaaca
      661 atcgttatta ctagccaagc taattcttcc cttgctaaac ttggcgatgt cgtttttatt
      721 tctatttctg aagaaacaga attccgatct gaagctttag cttcccgtat ttctcaacta
      781 agcattttgg actctctata tgtgattttg atgttttaca acgaaaaaga atcacgtcgt
      841 tctatcgcta aagttcgaca tactatttct gaaatcaaag aatagcacca taacagaaaa
      901 agcgaaaaat ccataaaaca aaaaaagcca ctaccaaata tttggtagtg gcttttttgg
      961 ttctgcaagg gtggtacaga acatcaatag tcggtgggtt agaacgtatg tcatcgcatc
     1021 tttggagaag gttgtattgg tattattcga gttacttgtt agatgcgata acaaagttag
     1081 tatacagtag taaaaaattt atcttttctt ttttttatta tttttgtttc ttattttcta
     1141 aaaaattaaa aaaaattttg gtgaattaag tatttccttg ataacactgg cttttggcct
     1201 cattttaggt tatcatttgt aattttattt cacagttgat atgattgtta tgtaaaataa
     1261 aaaagtttag gatacatcga atttttgggg aaattctttt gggggtatcc taaatctttt
     1321 ctattatatt cccatatttt ctattattgg tctagacgta tgatggtaaa ctttgtacct
     1381 atctgattgc tgaatcatta attgcttatc attagccatc tttgtttcaa gttcgctaga
     1441 tgatcatttt attcacatcc taacaaactt gtcacattta tagattctta atctatttat
     1501 catacaatta taaaaaagga ggtgctgctt atgaaagcta tccatatttc tttgcttttt
     1561 ttcgctagtg cattgatttt atttgctatt catctttgtt cccagcctta tatcaataac
     1621 aacggaatat tagtggaacc agcattcttt tgcttaccac tcggcttttt gtcccttctt
     1681 gcctccgcag gcttcggatt tgttgctttt tttaaacgat caaaacaaca aatctgactt
     1741 ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa atttcgaata
     1801 aaaggtgccc cttgccatct ctaactcctc agtcacaagt tacaatattc ataaaccatg
     1861 aaaagcttgt ttattgaaat tgctccttgt gacttggagc taagtgacag attacaacct
     1921 cttttgcatc ataagacttt actccttgaa aaacattact aacttttgat atgtttcttg
     1981 cgcttgttta ttgctgaaat caaactgata ttcatggttc aaaacagcat ttttctccgc
     2041 ataatctact tttgtgactt tcacccccgc tttctcaaat acagaagtta cttcttccat
     2101 ttgtttcgta aacgtatttg tatttccatc cgttaagaat gtgttttgcg tccaacgatc
     2161 cgcatgtctg accaaggcta aacttttgac cttctctgat tgttcaaagt ccttattatc
     2221 aaagtaagcc catccacatt tttcataaag aaagctagag attttatcac ttacttgagt
     2281 aagtgcagaa ggatccaaca gaccagacaa agaaataaac ttcgtaatct gcttgttctc
     2341 caaagtcgga tcaagatgat cagctttttt aatttcttca ttttgaagtg ctaacaaata
     2401 ttctccacta atttgtgcac ctgccgaatt tccaccaatc gaaatattcg accaatctgc
     2461 ttgatctttt atctcttttt tagctgcttg aatggcttcg ttcaattgga taactggtgt
     2521 aggatgtttt gcttccggtg atcgttcata atctatattt acgatgattt gctgcgtgtc
     2581 tgaacataga tattccaaat aatcacgaca atcattttta tcccctccta catatgcgcc
     2641 tccatgcatc cagaaaagaa tcggtaattt tttcttttcc actttcggtg tgtagatatc
     2701 catattatta tttttgtact ttgaaggata ggatatttct ttttttatat cgatttgttc
     2761 gattttttgc tggaaatcat ctggtcgggc atatacttgt tctttttcac ttgtgtcaaa
     2821 ttgttttcta acaagcaagc taatcggctt tggtgtgatt tgattaatca aaaatacagt
     2881 gactaaaata actacgatga taactggtac tgcaatcact attcgcttga accatttcat
     2941 tcatttaccc tctctttctt cgccaaatcc atgtgataat cgtgctaaca gataaaaatc
     3001 cacctgaata aatgagaagc gaatgtttct tttctccagt tttcggcaac attctttttt
     3061 ttgcatctgt ctttttttgt actttcactg tcatctgaat cggttgcgta tcatcacttg
     3121 attgatcctc tcgaggttca ttctttccct tattgtctgc attctctgtc ggatcttgct
     3181 tttttggtgg agctactggg atttttttgt aatagaaaat aatagttggt atcgtctcat
     3241 taaaaatccc ttcagtcggt ccttcaacat ttgctaattc gtatccttcg aatgtctttt
     3301 gctctgtcat ataattttct ccataagctc cgtaaagctg gatttcgtcc tggtctttga
     3361 gtagactttt attgttttca tccacatact ttacgtttat ctgatcatgg aacactactt
     3421 tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc aacttcctgt gcctttccat
     3481 gccctgctcc ttctactaat aaaagatctt tataccctcc tgataactca tataattttt
     3541 gcgcgttaga cacaggtact acatcatctg ctgttccatg gataatcaat ttaggcaatg
     3601 aatctgacat tccaatcgta ggtagagcag cttttgtatc caaatgcatc tcctgatcaa
     3661 aatagtattg tttcaaaagg cccataaata aatcttcatg ctcttgatcc ccaatgatgt
     3721 ctaactgatt ttttatggca tccggtagtg cgggggctac tgtctggctg tacgtaaaac
     3781 gtagttgttc atcaattgat gcaaatccgc aattttcaat aattcctttg acattgacag
     3841 gaatatcttt cgctaataca gacatcatcg tagctgctcc catcgaacca ccataaagta
     3901 aaatcttttg agatggccgc tctcgaactt cttgatttat ccagtaaagg acgtcatctg
     3961 actcatattg tccataggtc acataatcgc ctccgctatt accagtcgct ctggaatcaa
     4021 caaataataa attatacccg gccttataaa aatcattgta ttcttctgta acgatcccgt
     4081 tattccaatt ccctcgaaat ccgccatgga tcatgactgt tttatctgaa ccttgatcga
     4141 cataatatgc atatacatcc atcattttgc catcttgttt gtttagtaca gtttgatgaa
     4201 gttgttttgt atttggatag aagcctgtgt tttctgtagg cgttgtccca tcatctgtac
     4261 ctccaagcat cgacatcaac acactgtttt ttgcaaaagc gatatcgtac aatagataag
     4321 tagcaatcag atgtttgata atctctttat ctacaccata atcattaaca gcaagctcag
     4381 tgactaataa ctctattaat tttgacttga tgtcttctgt tggtccatta agtatttctg
     4441 tcatctgttt accgaagtaa ttcaaatatt ctttaccatt gaaattttca cctttaccga
     4501 tatcatcatt ttctgtccca ttctctttgt cacttacttt agcagaagag gattcgtcgt
     4561 ttgacatttt tgtagctgtt gcttcttctg tttgttggtt atctgtggta gcggtcgttt
     4621 cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc agtatttgtt tctgtttgtg
     4681 aagtactcga tttcgtttca tttccagcat cagtagtttc tgtggtttct acctcccgag
     4741 attccgattc ggaagaaatg ctctctccat atactgtcca ttgcgggacg cttgtactta
     4801 cagcaaataa gatgaccaac gctcccttta atacttgttt tgttctcaag ctattcactc
     4861 ctttttatgt ttctaggaga attatatgta aacgcaaaca ttttttatac aaaaaataaa
     4921 tggtttatcc cataaaaata gaacaggatt attttctcat tttacgtaat aaagcagaat
     4981 ggataaacca gtatccattc tgcttttttt aaacgaagaa acaacttatt gctccaaaaa
     5041 tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg attgtttcct ttgctttcat
     5101 aaaaggatta tcataagttg tcgttgctcc tctacacatc ataagctggt atcctaatcc
     5161 atgagcaaac ttcacagtcg tatctataca atactctgct tgtgccccac ataattcgat
     5221 cgaatgacag tccaattcag ttaataaaac actcaaatta gtcttataaa atgaattcgc
     5281 atgggttttt ctcacaaaat aactgtgctc aggaatagat aattccgaca atagctccca
     5341 tggttttgtt tgtggcacta attgagcatc ttcgtgttga acgaaaataa ctggtttccc
     5401 aatcgtttcg tatgttttga ttcttttatt cactaatgct gtcactgttt ctaattcgtg
     5461 tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg atcactacta ttgcgtcggc
     5521 tgtgtttgac atacttgtgc cttctttctt ttttcttgat ttaatagtat ttcttatcta
     5581 gctagttcca ttatacgtga caacattttc ttattttaca cctctaaatg gcacttttcg
     5641 actatttctt catgttatta tttcataaat acttagctat tggaggaatg tatgatgatt
     5701 aaattgtata caggttctgg ctgctcgtct tcaagacgcg caaaacgttg gcttatcgtc
     5761 catgatatac cgtttatcga acagaactta attcgtcaac cactaaccaa aaatgaattt
     5821 atccacttac taaaattgac aaacaacggc gtgaccgaaa tgctctctac tcattctatt
     5881 agttataaaa atcttgttca cagaatagat gaactcagtt tgagtgaact atatattgag
     5941 attcgtacat ccccttcttt attgaaaaaa ccgattatct acgaccaatc tgcaaaactt
     6001 ttgactggat ttagcgaaga taaaatcagg atgttccttt ctaaaactaa aagaaagcaa
     6061 gagctcaacg aactcttgct ttctggtcat cttgtcgatt ttaaagaaaa taatcttttt
     6121 gcaatgtctt aattttggca taaacaatct gcttttctta attaacgaaa tccaattttt
     6181 ccaatatgga tctatttttc tcattcctga tttttatgat ttcagctttc agacgcgccc
     6241 aatcattttt tgtaggttgt ccattccagt aaaatatctg tgtattcgtt tgatcatatg
     6301 cctttttgtc gataggataa tcgctaatga caatatcagg taactgatca gcggatgcac
     6361 tatattttat tgggcgttca gtcatagata atatcctttt ctttgcccat aattcctgtg
     6421 ttttaccata ctttgattga atatagaata tgacaggttg ttcgtgtttt ataaaaatat
     6481 ccctgactaa catacagtat tgaaaaatca accgtctgtt cttttcataa taagtattaa
     6541 gctctggaat tctgcgagct accccactaa aaaactgttg catgattgga tacatcactg
     6601 gaaatatatg ctgaaaatct ctgtcttctg ctgattttcc aaaagcatcc accttctttt
     6661 ttgtcccaaa aacccttgaa tacaaatgga tcattgccaa attgacatat agataatttt
     6721 tttctgaagt tgtaaaacaa atcttgaagt ttttttcaaa attttctaca atagacgatt
     6781 caatccagcc ccaactttct tgtactttat ctggagtatc caacaaatct tcttccaaat
     6841 aggtgttcat tacaccaaac ataaaataaa ggtaagcttg ctctgttgga gtgatgtgat
     6901 tcatcgacca gaagtttgct tcaaaaaaag gctctagtat gtaatgcgta aactcttctt
     6961 ttgttatcaa tggatgcaca aatttgttca tctcttctgg gacatacgta atcgagtggc
     7021 cttgtttcat tcgttgcaac gaaattctta gtaggctgat ccatttcttt ctatctatac
     7081 tacgcgtata tggcgcatgt ttcgcaaatt tttctaatag ttgacgttta ttttttatac
     7141 ttgataatat atcagaatga ttttcgtcga atgactgcca atacatcaag tgaaaaaagt
     7201 atcggatacg gtattcttct ccttctatcg gattaccttt tttcaagctg caagataaat
     7261 gatagttttt caaaatagta ttaagttctc ttaattttct ttgaatcgta cgcaaactta
     7321 tataattttt tgactaagat tttctaagga ttgataagta cctttgtaaa gtgaatccag
     7381 taaaatataa ccaatcgttc cctctaaaag tatagaataa actttactca gtaaaaactg
     7441 agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg
     7501 atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta
     7561 gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata
     7621 gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca
     7681 tactcctttt tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata
     7741 gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt
     7801 gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa
     7861 aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt
     7921 cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac
     7981 tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat
     8041 ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt
     8101 atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt
     8161 tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg
     8221 taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt
     8281 gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
     8341 ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     8401 gctcgtgttt tctgtgctaa tttcactgct cgtactctct gtactgcttt caccgctcgt
     8461 gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt
     8521 ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa
     8581 atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt
     8641 atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc
     8701 tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac
     8761 gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg
     8821 atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat
     8881 tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt
     8941 cttcatttct atcactctcc atataattac cttctatttg ataaaggaat tatataaaat
     9001 gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg
     9061 cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat
     9121 cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt
     9181 tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat
     9241 agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt
     9301 ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt
     9361 ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag
     9421 tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg
     9481 attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc
     9541 aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac
     9601 tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa
     9661 ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac
     9721 aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc
     9781 tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg
     9841 tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag
     9901 tgcattattg acaagaattc ctaaaacaaa accaacaaag actattaaaa cagatccaat
     9961 caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt
    10021 ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac
    10081 gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg
    10141 ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat
    10201 gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat
    10261 tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa
    10321 atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata
    10381 cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact
    10441 cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa
    10501 actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga
    10561 taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct
    10621 attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt
    10681 ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat
    10741 gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact
    10801 aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag
    10861 ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa
    10921 atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa
    10981 aacaatccgt aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa
    11041 tatattcaaa taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt
    11101 ttggaaattc cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt
    11161 tttttatttg taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaa
//
LOCUS       DAYYHS010000092        11201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000092 DAYYHS010000000
VERSION     DAYYHS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.092"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACHA6A_002448"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACHA6A_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACHA6A_002449"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACHA6A_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACHA6A_002450"
     CDS             1898..2059
                     /locus_tag="ACHA6A_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970586.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2543..3139
                     /locus_tag="ACHA6A_002451"
     CDS             2543..3139
                     /locus_tag="ACHA6A_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM8970587.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            3120..3566
                     /locus_tag="ACHA6A_002452"
     CDS             3120..3566
                     /locus_tag="ACHA6A_002452"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8970588.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            3909..4232
                     /gene="relB"
                     /locus_tag="ACHA6A_002453"
     CDS             3909..4232
                     /gene="relB"
                     /locus_tag="ACHA6A_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8970589.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            4552..4779
                     /locus_tag="ACHA6A_002454"
     CDS             4552..4779
                     /locus_tag="ACHA6A_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8970590.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4795..5961
                     /gene="hemW"
                     /locus_tag="ACHA6A_002455"
     CDS             4795..5961
                     /gene="hemW"
                     /locus_tag="ACHA6A_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8970591.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6196..7239
                     /gene="hrcA"
                     /locus_tag="ACHA6A_002456"
     CDS             6196..7239
                     /gene="hrcA"
                     /locus_tag="ACHA6A_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8970592.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7333..7896
                     /gene="grpE"
                     /locus_tag="ACHA6A_002457"
     CDS             7333..7896
                     /gene="grpE"
                     /locus_tag="ACHA6A_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8970593.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7947..9776
                     /gene="dnaK"
                     /locus_tag="ACHA6A_002458"
     CDS             7947..9776
                     /gene="dnaK"
                     /locus_tag="ACHA6A_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8970594.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9927..11093
                     /gene="dnaJ"
                     /locus_tag="ACHA6A_002459"
     CDS             9927..11093
                     /gene="dnaJ"
                     /locus_tag="ACHA6A_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8970595.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3945 a         1791 c         2288 g         3177 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     2401 taagaatagt aaaaattcac atagatgagg tataataatg atatttttat atattacaga
     2461 taacttgaca caaaatgagt ttttttcgat ttttcaagtt atctaatgca attatattga
     2521 tatcattagg agctgcattg ttatgaatcc aacattgatt actaagaaag aattactaaa
     2581 aaaattagat atttctactg gtgtattagc taatttaatt agaaatggca tgccaaaaga
     2641 gggagaaatg tttaatcttg ataagattat tacatggcga gaaaattggt ctaaaaacat
     2701 attaggtgag ttagaagttg gtagagtata tactaacaaa gaaatttcag agaagtttaa
     2761 atgttccaaa cagggtggaa tgagaagatc tcatcaaact aatactcttg tactgtttag
     2821 tgatcaaaca ggaagtaatg tttataaaga taagtggtta aatggtattt tacaatatac
     2881 cggtatgggg ctaaaagggg atcaagtatt ggataagaat caaaataaag ttttagccaa
     2941 ttcaaaaagt aattttgtaa agattcattt atttgaaaca tttaaaccaa aagaacacac
     3001 atatctggga gaagtatatt tagcaggaca aatatatact gttaatgaaa aagatagttc
     3061 gggcaacagc agaaaagtat ataaatttcc attagcttta ataaatcaag agcaattgat
     3121 tgaagataaa gatatataac caggaagaaa atcaaactag acatattcgt aatttatcta
     3181 atgctaaatt agaggaagaa gcaagaaaag ttagtaatta taataaaaaa atggctgcaa
     3241 aatatgttaa cgaagaggag ttttgcttct caaaaacaaa aatatataaa cgtaatccct
     3301 tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc caagaaaaag
     3361 ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa tatttgtcgc
     3421 aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt catgctagaa
     3481 ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt gaaaaaagag
     3541 aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc taatgtgagc
     3601 tatgccttct atataagact aagttctatt ataagtaacg aagtaattct cgttccttcc
     3661 tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct ccagtaaatt
     3721 ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt agttggctac
     3781 ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat ttactacaag
     3841 gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa aatcacatca
     3901 ggaaatgcat gagattcttt aagagaggca attatagggc tatgattacg gttcgtgtaa
     3961 gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt actttatctg
     4021 aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt caaacggctg
     4081 aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg gaagaatcct
     4141 taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga aattcagaag
     4201 cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat tcgttgttat
     4261 cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt atttttacaa
     4321 aaaaataata attggaatct agttaaaact cttgaagtga gagatttgac tagatttttt
     4381 tatgaataaa ataaggacaa aattgtttag gaagaatcat gagtgtaaaa aaatcatcca
     4441 agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta gtatgcgtat
     4501 atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc tgtgaaaata
     4561 gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc gattaccgtt
     4621 tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc atttttagag
     4681 ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct tgaacgcgat
     4741 tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat ttcaatgata
     4801 gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc attttgcgag
     4861 catatctgct attactgtga ctttaataaa gtttttttag aagggcagcc agtagatgag
     4921 tatgtggaaa tgctgctgaa tgaaatgaga ttaatgctgg aaagtcatcc tacagcgaat
     4981 ttggaaacac tttatgtagg aggcggaaca ccaacttctt tatctgctaa acaacttgat
     5041 cgactattat caggtgctag agagatcctg ccttttcaaa atggtaatga gtttactgta
     5101 gaagcgaatc ctggtgattt gacccgtgaa aaacttcaag tgatgaaaaa ctatggcgtc
     5161 aatcgtttat ccatgggagt gcagacattt gacaatcgct tattgaaaaa aattggacgt
     5221 aaacatactg cagaagatgt gtatcaaaca atgcaatttt tagaagcaga aaattttacc
     5281 aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg atatcgggat
     5341 actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc attgatcctc
     5401 gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact gcctgatcag
     5461 gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca tggacgtcac
     5521 caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa cctcatgtac
     5581 tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct tggagatatt
     5641 cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc aaatgaacgc
     5701 ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga aatgttctta
     5761 ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
     5821 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
     5881 gaagaagacc gtgtttattt gacaccaaga gggacttttt tggggaacaa tgtgtttgag
     5941 cgctttttat ttgaaaaata gtttgaagaa tatttgtttt ttatcgaaac ttgaaaagaa
     6001 aaagtttaga aagtttgaca atgaggtatt gaagtattga tataactgga tttacatcaa
     6061 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
     6121 ggagaagggc ttcttgctat atttataagt gaattagcac ttagaatata tgagtgctaa
     6181 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
     6241 cactatacga gtagtggcgt gcccgttggt tccaaaacat taatgtctga gggagtcaag
     6301 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
     6361 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
     6421 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
     6481 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
     6541 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
     6601 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
     6661 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
     6721 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
     6781 cttcgtacgg agattccgat gattcttcat aaatatttcc agacgacaga agggatgtct
     6841 cagttatttg acaccgttct tggtcatgtt tttgaagaaa aggtttatgt cagtggacaa
     6901 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
     6961 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
     7021 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
     7081 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
     7141 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
     7201 gctgattact accgctcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
     7261 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
     7321 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
     7381 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
     7441 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
     7501 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
     7561 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
     7621 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
     7681 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
     7741 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
     7801 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
     7861 gtgttaagac caactatggt cattgttgca caataattgt gcggatataa aacttacact
     7921 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
     7981 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
     8041 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
     8101 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
     8161 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
     8221 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
     8281 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
     8341 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
     8401 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
     8461 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
     8521 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
     8581 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
     8641 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
     8701 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
     8761 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
     8821 gtcaagcgtt aaaagatgca ggtctgtcac aatcagaaat cgacgaagtt attttagtcg
     8881 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
     8941 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
     9001 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
     9061 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
     9121 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
     9181 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
     9241 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
     9301 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
     9361 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtgaaa gatgcagaag
     9421 caaacgctga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagacg
     9481 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
     9541 aagtgaaaaa agcagaaaca gctcgtgacg aattaaaagc cgctgttgaa gcaaacaaca
     9601 tcgaggaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
     9661 aactatacga acaagctgca caacaacaag ctcaagaaaa tccagaagca gctcaaggcg
     9721 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
     9781 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
     9841 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
     9901 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
     9961 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    10021 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    10081 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atacgaccaa tacgggcatg
    10141 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    10201 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    10261 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    10321 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    10381 aaatctgtca tacttgtggt ggaaacggtg caaaaccagg cacacaacca actacttgtc
    10441 ataaatgtca tggtgcaggt acgatcaatg tagaacgcca aacgccactt ggacgagtga
    10501 tgagccgtca aacttgtgat gtttgtcatg gcacagggaa agaaatcaaa gaaccttgtc
    10561 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    10621 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    10681 gcggaccatt tggtgatttg tatgtggttt ttcgtgtaga agaaagtgat atctttgatc
    10741 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    10801 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    10861 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    10921 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    10981 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    11041 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    11101 agtaaaaata taaagtagac ttgcccaaat tttctatttc tagtaaaaac gctaagtcat
    11161 taataattaa aaaccagacg aacaatcata cgttcgcctg g
//
LOCUS       DAYYHS010000093        11081 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000093 DAYYHS010000000
VERSION     DAYYHS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.093"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACHA6A_002460"
     CDS             4..2376
                     /locus_tag="ACHA6A_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8970596.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACHA6A_002461"
     CDS             2437..3897
                     /locus_tag="ACHA6A_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8970597.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACHA6A_002462"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACHA6A_002462"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8970598.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACHA6A_002463"
     CDS             6128..6277
                     /locus_tag="ACHA6A_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970599.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACHA6A_002464"
     CDS             6352..6945
                     /locus_tag="ACHA6A_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8970600.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACHA6A_002465"
     CDS             6958..7857
                     /locus_tag="ACHA6A_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8970601.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACHA6A_002466"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACHA6A_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8970602.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACHA6A_002467"
     CDS             8723..8983
                     /locus_tag="ACHA6A_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970603.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACHA6A_002468"
     CDS             9428..9634
                     /locus_tag="ACHA6A_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8970604.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACHA6A_002469"
     CDS             9655..9885
                     /locus_tag="ACHA6A_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970605.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACHA6A_002470"
     CDS             9901..10338
                     /locus_tag="ACHA6A_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970606.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACHA6A_002471"
     CDS             10331..11038
                     /locus_tag="ACHA6A_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970607.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
BASE COUNT         4188 a         1845 c         2323 g         2725 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taaggacaaa a
//
LOCUS       DAYYHS010000094        10800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000094 DAYYHS010000000
VERSION     DAYYHS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.094"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..557
                     /gene="rpsN"
                     /locus_tag="ACHA6A_002472"
     CDS             288..557
                     /gene="rpsN"
                     /locus_tag="ACHA6A_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8970608.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            572..751
                     /gene="rpmF"
                     /locus_tag="ACHA6A_002473"
     CDS             572..751
                     /gene="rpmF"
                     /locus_tag="ACHA6A_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8970609.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            783..1160
                     /locus_tag="ACHA6A_002474"
     CDS             783..1160
                     /locus_tag="ACHA6A_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970610.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1181..1330
                     /gene="rpmG"
                     /locus_tag="ACHA6A_002475"
     CDS             1181..1330
                     /gene="rpmG"
                     /locus_tag="ACHA6A_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8970611.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1354..2337
                     /locus_tag="ACHA6A_002476"
     CDS             1354..2337
                     /locus_tag="ACHA6A_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8970612.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2367..2489
                     /locus_tag="ACHA6A_002477"
     CDS             2367..2489
                     /locus_tag="ACHA6A_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8970613.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2507..4030
                     /locus_tag="ACHA6A_002478"
     CDS             2507..4030
                     /locus_tag="ACHA6A_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8970614.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4054..4293
                     /locus_tag="ACHA6A_002479"
     CDS             4054..4293
                     /locus_tag="ACHA6A_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970615.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4486..4947)
                     /locus_tag="ACHA6A_002480"
     CDS             complement(4486..4947)
                     /locus_tag="ACHA6A_002480"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8970616.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5142..5360)
                     /locus_tag="ACHA6A_002481"
     CDS             complement(5142..5360)
                     /locus_tag="ACHA6A_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970617.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5478..7094
                     /locus_tag="ACHA6A_002482"
     CDS             5478..7094
                     /locus_tag="ACHA6A_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8970618.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7335..8675)
                     /locus_tag="ACHA6A_002483"
                     /pseudo
     CDS             complement(7335..8675)
                     /locus_tag="ACHA6A_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9113..9259)
                     /locus_tag="ACHA6A_002484"
     CDS             complement(9113..9259)
                     /locus_tag="ACHA6A_002484"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970619.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9298..9492)
                     /locus_tag="ACHA6A_002485"
     CDS             complement(9298..9492)
                     /locus_tag="ACHA6A_002485"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8970620.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9402..9734)
                     /locus_tag="ACHA6A_002486"
     CDS             complement(9402..9734)
                     /locus_tag="ACHA6A_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8970621.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10248..10439)
                     /locus_tag="ACHA6A_002487"
     CDS             complement(10248..10439)
                     /locus_tag="ACHA6A_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970622.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10533..>10800)
                     /locus_tag="ACHA6A_002488"
     CDS             complement(10533..>10800)
                     /locus_tag="ACHA6A_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8970623.1"
                     /translation="DQLPPYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPE
                     QIFLIEKFIFDKTDKEIGKVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3585 a         1709 c         2099 g         3407 t
ORIGIN      
        1 tgccgtttta agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata
       61 aaagtgaatt cttatttttc agagcctaaa ctgcatattc ttttaaatga tttgattatc
      121 tctatgtacc gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg
      181 ttatttattg ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga
      241 ttacgatttg aggtgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt
      301 cgaaaattgc taaagcaaag aaacaaatgg cgatgattga aaagtatgca gataaacgtc
      361 aggaattaaa agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc
      421 cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt
      481 tcggtatgtc ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa
      541 aaaaagcaag ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa
      601 ggcgaagaaa cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga
      661 cgaagcgaat ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg
      721 aaagaaagta attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca
      781 aaatggaaat tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga
      841 cccagcaatc gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta
      901 ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg
      961 aggaccaagg cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg
     1021 acgaggagtt atttcaatgt atcgatggtt attgggagat acctgttgat atgaaagaaa
     1081 atttagtttc acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag
     1141 catatcttga agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt
     1201 ggaatgtgtc gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc
     1261 tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa tttttaaaga
     1321 agtgaaatag ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct
     1381 gggtttttag gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac
     1441 cccagggaag aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa
     1501 ttattagttc atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt
     1561 cttcgcagtg atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa
     1621 caaccaattt cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt
     1681 caaacacttt taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact
     1741 gttattgata gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt
     1801 gtcatggcag atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa
     1861 aaattacaga gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat
     1921 agtgaaacga ttcaaaaaaa ggatttttat aatttgaaaa aatttaatca tcccttagtt
     1981 tttgaaagtg attcatcaga aaatcactat aatggcaaat actatcatca acaccatcaa
     2041 gtacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa agatttttta
     2101 ctccaatgga ttgactggct gattttaacc aaccaaggga gcctgtatcg ttttaaagga
     2161 ttgattgctt tacaagagca ggattttata gttgccatgc aagaggtaaa cgaacaggtt
     2221 aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat cggaaaaatg
     2281 ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag caaataatga
     2341 taagtagtga ttgggggtaa tttctaatgg agaaaacgga tatatctagt gcatatcgac
     2401 gacttaaaag cccgaatatt aaaactagaa aaagggcatt aaaaattatc aaagaacata
     2461 aacgaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga agaaaatcag
     2521 tgtaggactt attgggatag cagctctagg attattggga gcttgctcca gcactaatga
     2581 tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat tctatccaat
     2641 gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa tggttccagc
     2701 gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag ctcatgatgc
     2761 tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag
     2821 ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt tattgccagg
     2881 tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac tagatcctca
     2941 tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg atcagttagt
     3001 taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt atttgactaa
     3061 actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac aaaaaagttt
     3121 tgtgacgcaa cacgcagcct ttggatattt agctttggat tatggattga aacaagttcc
     3181 tattgctgga ctaagtcctg aagaagagcc atcttctggt cgattagctg aattaaaaga
     3241 gtatgttaaa aaaaataaga tcaattatat ttactttgaa aagaatgcta atgataaaat
     3301 tgcaaaaact ttggccaacg aagctgggat aaaattggaa gtactgaatc ctcttgaaag
     3361 tcttacaaaa gaacaaatgg ataatggtga agattatgtt tcagtaatgg aagacaatct
     3421 aaaagcgtta gaaaaaacaa ctatggttgc tggtgaagag gtagttccag aaaaagaagc
     3481 gaaggatgaa aagacagttg ctaatgggta ctttaaagat gccgatgtga aggatcgcga
     3541 attgagtgat tatacaggcg aatggcaatc tgtttatccg ttgttaaaag atggaatttt
     3601 ggatgaagtt ttcgattaca aagcaaaaat caataaagac atgacagctg ccgagtacaa
     3661 agattattat actactggct ataagacaga tattgatact atcaacatta aagacaacac
     3721 cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg ttggatacaa
     3781 aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc
     3841 aggtgctggt cgttttaaat atattcaatt tagtgatcat ggaattgcac caagtaaggc
     3901 agagcatttc catattttct ttggtggaga aagtcaagaa aaactctata atgaattgca
     3961 taattggcct actttctatc cagcttcatt gtctaaacat gaaattgctc aagaaatgat
     4021 ggctcattaa aattcaattt ttggaggaaa tacatggtaa gaaaagtatt attgtctatg
     4081 gattccaaga atattgatgc ttatcggcgt tatcgatttg caaagcaaat gagattaaat
     4141 gggagtacat tagctgatat tgctgcaact ttacatgtta gtattgacac agttcataga
     4201 gtattaaaac gggatcctga ttgttgtttt atcaaggtca atactggaag aagtagacgt
     4261 ttagcaaaac ggcataatag cttggccaat taattgataa taccagagta gatcattttt
     4321 cccttatttg gatttgatct gctttttatt atgtcttgaa ttgtcaaatc aagcgcaaca
     4381 ctttatcaaa gaagacttca taaggtgttc tccaatctaa acactttcta ggtctgttat
     4441 tcagtttttc aatcatctga tttatcgttg agttatctac cgtagctatc tctttcgatt
     4501 ttggcaagta ttctcgaatg agtctattcg tattttaatt tgttcattgt tgccaagggc
     4561 atgaggatct ggaaaataaa agggcatatt attcaatgct tctgtaactg cgaaatgatt
     4621 ggagaattct tttcctctat ctggagtgat cgttttgacc gcctcatttg gaatactgct
     4681 taaaagttca atcattttct cgctcacata agcggctttc tttctgggga ccttctttcc
     4741 tgctaaaagg aagcatgatt ttctgtctgt caaagtcact aaacaagtac caccagtttt
     4801 gccagctaca atatcggctt tccaatgacc aaaatcactt ctatcatctg cggattttgg
     4861 acggccatgg atcgaatgag taaggcaaat ctttccacga gactcgacat gattttttgt
     4921 atgtcttgtt tttcctcgat ggtgcaatga acggatcagg cctttattgc cgtgtgagag
     4981 gggttctgta tcgaaaagat cattgcaaat tgcacgttag atcgtgataa aactaattga
     5041 aaagtccata tgttcatgct tcaaacgatt cgagatttcc tctggcgacc actgaaaatc
     5101 gacaaataaa tgacgaacaa ttgaccaaac tcacggatca tttagaatgt gataacgtcc
     5161 gcattttttt cttcgataag cataattttt ttccgctttg gaaggtgaat agtttttttc
     5221 ttgtttcttc tcagttcatg taaaatagta aacgggctat gctgaatgtc gttgccaatt
     5281 atactaatag aaaatccttc ttcgtgaagc agaaagattc tttctcgttc gagtatagta
     5341 agatgacgat actggctcat aattattttc cttctaggag tgttctcgac aaacttatac
     5401 tagttggata tcgattatga ctcattttta tgtgttgcac ttaaagtgta aattcaaggt
     5461 atagaaagag gttccctatg aaaaaaatac ctattacaat cttaactggc tatcttggtt
     5521 caggaaaaac gatacttcta aatcatattc tgtacggtga tcacggaaag aaaattgctg
     5581 tcattgtaaa tgacataggg gaagtaaatg ttgatggggt attagtcgac aagaaaggtt
     5641 ttaaaagaac tggagaaaaa cttgtttcga tgccgaatgg atgcatttgt tgtacattac
     5701 gcgaagattt gattgttgaa ctagatcaat tagtgcaatt ttctgacttg aatcaaattg
     5761 ttattgaagc ttcaggaatc agcaaaccaa ttccaattat tcaagcaatt atattagcta
     5821 aaaacccttt agacattgat ttgacccaac gattagagat tggtgccatt attatagtag
     5881 tggatgtaaa acgtttttgg gataatttcg aaacgggtga aaagattcag gaaagaaaag
     5941 ctgaaggtaa tgtagttgaa gaagaacagg atattcgtgg cttgttaatt gatcaattcg
     6001 agtgttgtaa catattactt ttaaataata gtgacttagt gtcaaccaag acgatttaca
     6061 aaattaacgg tctaattgct aagttacagt ccaatgccaa aataattagg acaattaaag
     6121 gtgaagtaga cttatcgaaa attctaaata caaatctttt taatatagat gagacttcct
     6181 attcagtggg ctggttcaaa gagttagagc ttggctatca aaatcatatt cctgaaacag
     6241 aagaatatgg tatctcaagt tttatctatc gaaatcggtt actccttgac gcacaaaaat
     6301 ttgcccatct tacggataaa aattttcctg gcaatattac aagagcaaaa gggtttcttt
     6361 ggtttgccca acatagtgat ttttcgacga tctttgaaac atcgggtagc tataaaagta
     6421 ttgaatcatt aaattattgg gtagctagtt tacctaaaat tgagtgtcaa atgattatac
     6481 aaaaaaatcc cgaattgaag gaagcctggc atccgttgtt tggtgaacgc attaataaac
     6541 tagtttttat tggtatgaag atggatcaac aatgtattaa ggaacaactt gagggttgtt
     6601 taactgattc tcttaatgca gataatttcc gaaaaatttc accatttcca tggaaaaagt
     6661 ctaatatgtt tagccagata tcgttaatga aatttttaag aaaagctggt gtcaagatag
     6721 gcgatgtcat aattattggc attttgatta ttacctcttt tattccctta tttgtattct
     6781 ctatagaaaa ggagaatgaa acaactgaag atgctatcaa atatgccatc gttcgaattg
     6841 atggcaagga aatggatcgc ttcgatttgg ataagatcaa tcataaactc gtgaattatc
     6901 atcctgcgaa gggacaatat aatattgttg agattaaaga tggaagaatt cgagttaaag
     6961 aagataatag tcctgatcaa atcgcggtta gaactggatg gatctcaaaa cccggtcaaa
     7021 cgagtatttg tttaccccat aagttagtta ttagtattga gaaaaaagaa agcacagatt
     7081 actacattta ctaatctgga aatagatgag gaggttttta ataagaaaaa gatattatta
     7141 ttaataatag caggggcttt attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg
     7201 gtgacaacag gcacaagtat ctttaacggg acctgcaatg gtttagttaa cagtcggtta
     7261 gattacttat tttggctctt cgtcaatgag tgttggtgaa gaaattcaat agaataatcg
     7321 agatactcac acggctagac agctatcttg tctagccgtg ttgttgttct ggctttagtc
     7381 agttgggtag tattcttagt tttcttaaaa tattttcctc gatataaaaa gattcgttct
     7441 cggtagtttt gaaagtttct atagccatag gcaattcgtt ttagtacttt gatatggtta
     7501 ttcaaacatt ctatagggcc gttggaatag ggaacgcgca acgctcgttt gatttctgtt
     7561 tggtatttct tgaaggtcgt gaacactggt tggtagtgct ctggcaactc cttgtagtct
     7621 tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgataacgga aagaaaatcc
     7681 tggtagactt cgtagcctcg ctttaaggaa tcatcataag ataatagccg atcgactatc
     7741 tctgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg acctttcaat
     7801 tcccacgtat tcttttgaag tagtttccag tatttcttta agtgcttcga ccgacgatcc
     7861 ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaagcccg accaatgtgt
     7921 tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat
     7981 gtgcgatagg gtgtatagaa atctgaaacc actagacgca cttgttcacg gacttttata
     8041 gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc
     8101 agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac atttttcact
     8161 gatttgaatt catcgaaaca caccacttct ggtaacagct ggctcaaaga aatggtttta
     8221 ggccgaaggc tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt catacgtgcg
     8281 atcaaagaca tagaaagagg ttcgcttaat agctcaagaa tggcttgttt gactctgcga
     8341 gcaatggagc agcgacgatc actaactgaa tcttctgcta aaaaagtctt tccacagtgt
     8401 ttacatttga agcgctgttt gttaatccgc agaatggtct tgtagttact gacatcattc
     8461 aataagatgg atactttttt ccagccctat ttaatgatcg tttgttcatt ttgatagtgg
     8521 cgatgaaagc attcctccgg ttgataggtc aatgtgccat caaaaacaaa acatgtttcg
     8581 tcattgattc gctctttgcg aaaggcatct tcatgaaaga taagatttag gtctagaata
     8641 tctagtaact ctttggtaaa ctctgaatag gacatcttga aacactcctt tgaatggttt
     8701 tcgtcgactt tattctaaag gattttaagg tgtctgtttg cgtttattag aaaaaagaaa
     8761 aagtattggc aacagaaggc gtcaccaaca ctagatatta tagatccctt attttcttcg
     8821 cttcaaattg tcaaggagtc atcataaatt ttgtatggat tcttttgttt gggtagtagt
     8881 cttctaaata atcttggatt aacaccttca tctctatttt acttttatat ttgttggagt
     8941 cgattaattc tcatttgatt gatcgatgaa acgcttcaat tactgcattg tcaacaggag
     9001 tcccagtttg gctcactgaa tgtgtgatgt ggtggtcgtt caacagctct tgataaataa
     9061 aagaccgatg ctggctacct ctatcggaat gaattaccgt aggcaattct ccttaataaa
     9121 tttgtaatgg acggacgaca atctcttttt tcatgtgtga atctaccata tagtaacgta
     9181 gaatacgagt tgctaagtca atataggtgg acaaatagag gcgaccgtca ttacatggaa
     9241 tgtaagtaat atccatcacc caaacagcat caatttggtt ctgttcaaat tatcgattca
     9301 acattttttt accatttctt tgattgtttt ttctctacca tgagaagatt tgcgtcgatg
     9361 aaatcctctt gtgcataaat tcatttcacg cataagaaca gctacaacac gtttattgat
     9421 ttcgattcct tcatcgtaaa gtttttgagt gatccctgga gatccgtatc gatctttgtg
     9481 ttcgtagaac actcgtcttc ctcttttagt cgtttatttt cctgttcaag aatgtagaaa
     9541 tctgctttat caggtaatct ttttccttgt ccagcaaaag catttttccc gtatcgacga
     9601 tattctgaaa cccatcggga aattgttgga cgagagatat tgtattcttc agcaattttc
     9661 ttggtagtta cttttttctc aaggatatcc tgtgaaatct ttagtttaaa tttacgatca
     9721 tatgtttttc tcaatagagt tcattccttc cggtaacgta actgatagtc aagaaaagtg
     9781 tatcatagcc caacttagta tcagggtttg tcaactaaac tgtggaagtt gatagataag
     9841 agtttttaac aaccaaaatt ctcttgacta tttttgaaat cagacaaatt ttgattggac
     9901 acatagttga ataaatctac cgtcgttacg gaaggtaaat cgcatgcttt tcaattctag
     9961 aggagaagtc ctcgttgaag taccatagcc cagttctgga ttcgacctcc tgcccacttc
    10021 atacgtaaat aaagtaattt taggagtgag actgtgacaa aagttatttt taagctaaaa
    10081 acgaattaaa aaacgttgat aattaaaatc attcgtttta gattctatga atcattatag
    10141 aacgctgtaa aatcgatact ttataattat aaagaagata gtttcaaatt tattgttcga
    10201 aatttgaaac tatcttttta cttatgtcac agactccctt agttgagtta ttctttaatt
    10261 tcgaatttcg agatagctag atatattgca agcatacatt cttgataaca atcttgatca
    10321 tattttccag ttctccagga ttgcttatga atcaaagagc taaatctttt gagaattgcc
    10381 tcaacagctt cttcatttcc agcctttgct aaaatgaaat cttcttttaa agaattcata
    10441 aatctacctt cttcccatgg acttataatc tatttctttg ggaaggagga gaacgagcaa
    10501 agaagcatac aaaattagtt attatgcaga tattaaacca gcattttttg aatttttttg
    10561 taaagtcgat gttttaaatt tgtcgcaccc tgtcttgtaa tccccagaac ctttccaatt
    10621 tctttatcag ttttatcaaa gataaatttt tcgataagaa aaatttgttc aggttctttc
    10681 aacaaaggaa ggatttttgc taattttata tcttcaacaa ggagagtcac atttaaaaca
    10741 ttaatcggtt gtttaaattt tatagtatct ttagtaaaca aatatggtgg gagttgatca
//
LOCUS       DAYYHS010000095        10493 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000095 DAYYHS010000000
VERSION     DAYYHS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.095"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHA6A_002489"
     CDS             complement(79..2199)
                     /locus_tag="ACHA6A_002489"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8970624.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHA6A_002490"
     CDS             2429..3607
                     /locus_tag="ACHA6A_002490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8970625.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHA6A_002491"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHA6A_002491"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8970626.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHA6A_002492"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHA6A_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8970627.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHA6A_002493"
     CDS             complement(4960..6213)
                     /locus_tag="ACHA6A_002493"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8970628.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHA6A_002494"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHA6A_002494"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8970629.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHA6A_002495"
     CDS             7790..9136
                     /locus_tag="ACHA6A_002495"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8970630.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHA6A_002496"
     CDS             complement(9444..9830)
                     /locus_tag="ACHA6A_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8970631.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHA6A_002497"
     CDS             complement(10098..10247)
                     /locus_tag="ACHA6A_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970632.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3346 a         1894 c         2106 g         3147 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agc
//
LOCUS       DAYYHS010000096        10460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000096 DAYYHS010000000
VERSION     DAYYHS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.096"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..635
                     /locus_tag="ACHA6A_002498"
     CDS             12..635
                     /locus_tag="ACHA6A_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970633.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1085..2134
                     /gene="trpX"
                     /locus_tag="ACHA6A_002499"
     CDS             1085..2134
                     /gene="trpX"
                     /locus_tag="ACHA6A_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8970634.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2131..3012
                     /locus_tag="ACHA6A_002500"
     CDS             2131..3012
                     /locus_tag="ACHA6A_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8970635.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3015..3785
                     /locus_tag="ACHA6A_002501"
     CDS             3015..3785
                     /locus_tag="ACHA6A_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970636.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4064..4918
                     /gene="aroE"
                     /locus_tag="ACHA6A_002502"
     CDS             4064..4918
                     /gene="aroE"
                     /locus_tag="ACHA6A_002502"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8970637.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5096..6259
                     /locus_tag="ACHA6A_002503"
     CDS             5096..6259
                     /locus_tag="ACHA6A_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8970638.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6376..6786
                     /locus_tag="ACHA6A_002504"
     CDS             6376..6786
                     /locus_tag="ACHA6A_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8970639.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6960..9188
                     /gene="topB"
                     /locus_tag="ACHA6A_002505"
     CDS             6960..9188
                     /gene="topB"
                     /locus_tag="ACHA6A_002505"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8970640.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9359..9643
                     /locus_tag="ACHA6A_002506"
     CDS             9359..9643
                     /locus_tag="ACHA6A_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970641.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9803..10039
                     /locus_tag="ACHA6A_002507"
     CDS             9803..10039
                     /locus_tag="ACHA6A_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8970642.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10036..10434
                     /locus_tag="ACHA6A_002508"
     CDS             10036..10434
                     /locus_tag="ACHA6A_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8970643.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3640 a         1763 c         2217 g         2840 t
ORIGIN      
        1 tagaaaagaa ggtgtttgtt atttgggaag tagttaaatc gagagtacaa gaaatgaatg
       61 aacatttctc agtgtcattc gcagaaaatc caagtaagta ttttcgacgt cttgtttttc
      121 tgctcatctt atcattgctg ttaacagttg caatgttttt attgccggaa aaaatccgag
      181 aagagcaagt aaatagtctc gtttattcgt ccataccaac cagcaaattg aaaccttttg
      241 attacagcaa agctgatcag aaaatcaaac aaagccgggc gatatcggtg atgttcagca
      301 aaccgaacgg gaaaacctat caggacatgc ttgatgtttt taatgatcct gagcttatgg
      361 aagaattgaa tcgaccgatc ttttattatc cgatcgttta tgatgtacat gaactggaac
      421 aaaaatataa catacgaacg gatgaagtga catttatttt ctttgatgga ggaaaagaag
      481 caaatcggat aactgcgggt aagggaggaa taactgattt tgataaggag ttgatccctg
      541 aactgaatcg acttccgtta gccaatatca aacaattaga agaagaattg gatagaacgg
      601 gagtaacttc ccaaaattcc gagattgcta attgagacat tggcgcgatg aaacggtggg
      661 tctatatagc gctattttta attatcagaa caagtgatgt ttgatcttca aaataacaaa
      721 taaaaaatta aaaaatatag ttgacgaaat tcaaaaattc gattagactt aaatcaattc
      781 ttaaggaaag ggtgacctgt aatgaactta tcaaaacgta tttcagcaga aaacaaaaaa
      841 aattggcgca gctggcgtag ataagttgta ttaatggtga gactttccca tagatacaac
      901 tttttagagt gcatttctaa agcttgtatc tatgctagac atgaacattg actgccgcat
      961 agattagaat ctatgcggtt ttttgtgtaa agacaatgac cgctttggcc attgtctttt
     1021 tttgtagact ggatacttgc ctgattataa taaataaaaa gattgacttt gcaggaggaa
     1081 aaaaatgaaa aacaaacgat taataacagt tgtagtaatt attttagttt atttagcagg
     1141 tgcatttatt tatggaaaag cctcaggcga aaaagaaagt acttcgacga accagacaaa
     1201 aaaaacaaca gttgttgggg tattgcaata cgtgagccat ccggcattag atgagattta
     1261 tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa atcttgagat
     1321 caaatttcaa aatggacaag ccgatcagag caagctagcc acaatgagtc aacagctagt
     1381 acaatctgat ccagatgcct tgattggaat cgcaacacct gccgctcaat cattagcaaa
     1441 cacgacttca tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg gggctggatt
     1501 agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc agccgcctgt
     1561 tacttcacaa atcaagcttg cttctgattt gttgccaaat gcaaaaaaag tcggaatctt
     1621 gtatgcttct tcagaggata attcaaaata ccaagtagca caagcagaag aagctgcaaa
     1681 aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc actaatgaga ttgcacaaac
     1741 ggttcaagtg atgagccgac aagtcgattt tatctatgtt ccattagata ataccattgc
     1801 caatgcgatg caaacagtgg taaaagaagc aaacaaagca aatatccccg tcattccatc
     1861 tgtggataca atggtggaac aaggtggact agcaacaatc gggatcaatc aatatcaatt
     1921 agggcttcaa agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa aaccggaaac
     1981 gacgcctgtt tatacatttg atcaagggga tacagtgatc aatcaatctc aagctgatcg
     2041 cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta cagatgactc
     2101 gcagcaagaa acatctaagg aggatgacaa atgatcgtat cagctatttc acaaggcatg
     2161 ctttgggcaa tattaggatt agggattttc atgacttatc ggatcttaga ctttcctgat
     2221 atgacgacag aagggtcctt cccattaggt gctgctgtct gtgtgacagc tattactcat
     2281 ggggtagcgc cgattgtagc tacattatta ggcgtaggtg ctggcatgat agcaggactc
     2341 gttacggggt tactttatac aaaaggaaag ataccggtta ttttagcagg gattttagtg
     2401 atgtcaggat tgaactcagt tattttgttt gtgatgcgaa caccaaatct ttctttattg
     2461 aatcatccga ttctacaaga cagcttccat cgctttgggt tacctgatta ctttgacaca
     2521 gtccttcttg gcttcatctc tttgggcatc ttgattgctg tactgctatt cttcttcaat
     2581 acagatttgg gtcaaggcta tattgcgaca ggagacaacg aaacgatggc tcggtcgtta
     2641 gggataaaaa ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg tgtcattgca
     2701 ttatccggtg cgctgattgc ccagaacgac ggatatgcag atgtaaataa agggattggc
     2761 gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag tgatcttcca agaattgacg
     2821 ctagcagaaa gattgattgc tattgttgtc ggcagtatca tttatcagct attgatttta
     2881 gtagtaatca aactagggtt tgacacgact tatctgaagt tgttctccgc tatcatctta
     2941 gctatttgtc tgatgatccc gcaattgaag aaagcactga acttaaaaac gggttttgag
     3001 aaggaggcat agtaatgact gatatattga caatcaaaaa tggcagaaaa gtcgtaaaca
     3061 atggaatgaa tgaagagaaa gtactattgg atgaaattaa ccttacaatc agagaagggg
     3121 acttcatcac tgtccttggc gggaatggtg ctggaaaaag cacactgttc aatacgatcg
     3181 ctggtacgct tcaactcagt caaggatcga tctcttttaa agatcagctg atcacaaaag
     3241 attcagaaga aaaaagagca cgattccttt ctcgagtttt ccaagacccc aagatgggaa
     3301 cggctcctcg gatgacagta gcagaaaatt tgctacttgc acaaaaaaga ggtcaaaaac
     3361 ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga ctatgtcaag
     3421 aagtcggtaa tggcctagag caacacttgg atactccagc gggtgaatta tctggtggtc
     3481 aacgccaagc attaagcttg ctgatggcga cgatcacagc tcctgagtta ctattattag
     3541 atgaacatac tgcagcttta gatcctaaaa cagctaaagc attaatgcga ttaacaaatc
     3601 agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg gaagacgcct
     3661 tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa gatatttctg
     3721 aaaaagaaaa gcaacatttg acaatgggcg accttttgca gttctttgaa gaagtagtgg
     3781 attgatttcg acagaaaaat tcctttcagt agaaggactt gaaaactcat tgacatcttc
     3841 ctttgattca tcctatactt aaaacaataa acaattaaac atatttgggt gttttgtagg
     3901 atcacgtagt caatgatttt aacaattaaa tagaatattc tttgctctga caagtaaacg
     3961 atgagaaaag aatttgctac tttgccacct agacgtttcg tactttttcg tatcaaacgc
     4021 taagtggctt tttttattga agtaagaaca aaggaggaat aagatgattt ctggacaaac
     4081 caaattagct gggctctttg ctgatccggc ttctcacagt ttatccccat tgatgcacaa
     4141 tactgctttt gaagcaaaca acatcgatgc ggtgtattta gctttccaag taaatccaat
     4201 gaatttgaag caagcagtcg aaagtattcg aacttttgat atgttaggtg taaatctttc
     4261 gatgccgaat aaaacagcgg ttattcctta tctggatgag gtaagccagg aagcgcaatt
     4321 gatcggagca gtgaatacga tcgtgcacga aaagggtcgt ttgattggct ataataccga
     4381 tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa agaaaaaaat
     4441 gactctactg ggagctggag gtgcagcaaa ggcagtcact gttcaagctg cgttagacgg
     4501 cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag taaaagaaac
     4561 attcgaacaa ctcagctatc gaacttcttg taagataaaa gttcgtgact ttgccgatac
     4621 gcatcaatta agaaaagact tgaaagaaag ctgtcttcta gtcaatgcaa cagatatcgg
     4681 gatgggaaag aaaatcaatc agtcaccgct cttagatata agcatgatgc atgagaatct
     4741 agcggttttt gatttgattt atgcacctag agaaacacgt ttattaaaag aagcaaaaaa
     4801 agtaggagca aaaggataca atggacttgg catgttgatc taccaaggag caatcgcgtt
     4861 tgaattatgg acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt ttgattaaaa
     4921 aaggataata gataaagatc ttttattctc gcataaacgg tctcaagaaa agtaatcctt
     4981 tatgtatcga agcatttatt tcgttatttt ttattaaatt atcaaaaaat gataggcttt
     5041 tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa ccacaatgag
     5101 tcggatggat cgctataagg acatccataa gaaggcgaaa cctctgaagc gggatacggg
     5161 gagtattttt gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg aagatgctac
     5221 ccgtatttat gaaagagagc agcattcatt tgaacatttg aaccaatacg agcaacagat
     5281 atacagagat gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt tcaatcggca
     5341 aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa tcattctatc
     5401 gattttgctt gttttgatcc tttattcagg tatctcattt tttattggta aatatgtagc
     5461 tgaacatgac acaagcttag aggcagcgac ttcagaaacc tttcatgggc aaaaagcgac
     5521 gaatggtgcg cataatattt tgatccttgg aagtgataca cgcggagaag atgccgggcg
     5581 agccgatacg atcatggtct tacagttgga tggtccttcc cataagccga aactgatttc
     5641 ttttatgcgt gacagcttcg ttacgatccc aggggtagga caaaataaga tcaattcagc
     5701 ttatgcatac ggaggagccg atctagtgcg tcaaacttta gtagaaaatt ttggaatcga
     5761 ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg atgccttatt
     5821 catgaacgga gtgaagatcg atgcagaaaa agatctgaat ctggatggtg tagatattaa
     5881 aaaaggggtc cagaagatgg atggacatgt tcttttgcag tacgcacgtt tccgaatgga
     5941 tgaacaagga gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg ctattttcag
     6001 ccaattgaag aatccattga atctgatccg tgcaccttat gcagctggta aagctttagg
     6061 atatacatcc acagatgtca ctgcttggtt cttgacgaaa aacctctttt cgattgcacg
     6121 aggagctggt ggcattgatc gaataagtgt gccagtagac ggttcgtgga attatgggca
     6181 aagtgattat gctggcagta ttcttgaaat tgataatcaa atgaatcaat ctgctattac
     6241 agattttttg aataaatgat cataaaaaag atcaagaaga agctgcgcaa ggcttttgtc
     6301 atgaatcaat caaacgattc actcgtaaaa aacgctttct tatggtagat ttaaccaaga
     6361 gaggagctgt ttacattgga tatctatctg aacttcaaaa atgagtcgcg tgaagccatc
     6421 gctttttatg aaaaagtttt tcagactgaa gcatcaggta tcaccacata cggtgaaatg
     6481 cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac gaatgcacgg
     6541 ctgaacattt atgatacatt agttatgttt gcagatacgc cagaaggaat tggtcctgaa
     6601 ttagttgtgg gaaataatgt ttctttagtc atccagacaa gaaatgaaga agaaacgaag
     6661 cgcctatttg aaagcttagc tgagggtggt aagattgaga tgccactgga tgctgttccg
     6721 tgggctcagt tgtatgggat ggtaactgat cgatttggca tcaactggca gattaaccaa
     6781 gagtgacagc taaagatgaa tattttctgg aaacatcaag cagcaaacgg atcatcgttt
     6841 gtttgcttga tgtttttgtg tctatttcga ctatacggta tctttttttt attgccagat
     6901 gcgaaaatat gttccataat agagaagtcc gcaaaaaatt aagtaacgga ggtcagaaga
     6961 tgaagacggt gatactagct gaaaaacctt cacaagcaaa agcctatgcc gaaaccttcc
     7021 aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt gctggtgaag
     7081 taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga gcttatgaag
     7141 agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc ttccgttacg
     7201 aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg cagtcagccg
     7261 ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg tggtcgatta
     7321 ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg atcaattctc
     7381 ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag aactattttc
     7441 caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga atgaatggaa
     7501 gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt tctcttggtc
     7561 gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc aaacacttca
     7621 aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg cagttttctg
     7681 gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag gaaatagaaa
     7741 aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg aaaaaggaaa
     7801 agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag atgaatcagc
     7861 taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac gaaagcaagt
     7921 ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat cagtatctgg
     7981 ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag acaactgaga
     8041 tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat gcaattattc
     8101 cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta cagcaagcaa
     8161 tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat atctacgaag
     8221 agacgaccgt aatgacgggc gtacagcaat taaagctaaa aagcatcgga aagatcccat
     8281 tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa gctcagactc
     8341 ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg gagaaagaaa
     8401 cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa actgcgggaa
     8461 aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga atagggacag
     8521 aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc gaagtcaata
     8581 aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta gccaatgaac
     8641 cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga aaaattggta
     8701 aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc ctacatttat
     8761 tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt agcgggacac
     8821 gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca aaatgtaaaa
     8881 agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa tgcgacttca
     8941 aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga gatttgcttt
     9001 caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg aaattcgatg
     9061 cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg atcgaaaaca
     9121 gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat gaaaacgaag
     9181 tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga gaatataaat
     9241 accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag gctgatttta
     9301 tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa attatcttat
     9361 gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg attacgggac
     9421 gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga ttttatcgac
     9481 tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa gcatgctaaa
     9541 gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca ttatttattt
     9601 tctaccagtt attacacaag tatttcatca atattttggt taagaatacg agagtttgtg
     9661 acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa agcaaaataa
     9721 aagtatatac attgattaaa agtatatact tggatataat gtacttacta tagaatgaga
     9781 atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta gaaaatctgg
     9841 caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg tgcagacagg
     9901 tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca aagcacaaga
     9961 agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag acgcgttaaa
    10021 agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat caacgcgaaa
    10081 gttattttac gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa tgcattagat
    10141 atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc tgaaatctat
    10201 gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca taatggcaat
    10261 aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg tccagttgct
    10321 ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc attggatttc
    10381 gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa atgaacgaat
    10441 taaaaacttt atttctcaat
//
LOCUS       DAYYHS010000097         9718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000097 DAYYHS010000000
VERSION     DAYYHS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.098"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..3349
                     /locus_tag="ACHA6A_002509"
     CDS             71..3349
                     /locus_tag="ACHA6A_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8970644.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3890..5797)
                     /locus_tag="ACHA6A_002510"
     CDS             complement(3890..5797)
                     /locus_tag="ACHA6A_002510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8970645.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5927..6802
                     /locus_tag="ACHA6A_002511"
     CDS             5927..6802
                     /locus_tag="ACHA6A_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8970646.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6882..7856
                     /locus_tag="ACHA6A_002512"
     CDS             6882..7856
                     /locus_tag="ACHA6A_002512"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8970647.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8036..9181
                     /locus_tag="ACHA6A_002513"
     CDS             8036..9181
                     /locus_tag="ACHA6A_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8970648.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9318..9662
                     /locus_tag="ACHA6A_002514"
     CDS             9318..9662
                     /locus_tag="ACHA6A_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8970649.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3171 a         1797 c         2055 g         2695 t
ORIGIN      
        1 attgacagta aaaataaatt aggtaatact ttaagtaaac gctttctaat ttgttacggg
       61 aggaatacac ttgttaaaaa aacgtataaa atacagtgcg ctaatgatga atctccttat
      121 gctcagtacc ccggttttaa tttctgtcgt tgaagttgct gctaacgaac aacagtctgt
      181 gaacgaagaa aatgcacacg tgaacattga agggatgtca acaatggagg aaataccaag
      241 aactagtgaa gatatggata cggaagaagg aaatgatcct atgatgaaag aatcagaaaa
      301 tgaaaagaca aaaaagcaat ctgaagaagc aaatgaagag tggaaagggg ttgttttcgg
      361 agaaagcaca agtgctagta ggtatgccat tgaaccatta gaagacggcg tgcggctctc
      421 ttccacgaat aacggcggaa aattccagtc aagcggatca gatggaatga cctattatta
      481 tcaagcaatc cccaaagaca tcaactttag gatgcgggcg acgatcgaaa tagaatcttg
      541 gacctatacg aatgcccaag aaggatttgc tttaatggta agagacgcag taccaaaaaa
      601 tcatttgttt gggcaggcat tttattcaaa ttcatttgcc atactaggca gtcggatcga
      661 atatgtctgg gactcggata gacaagaagt ggtcaacacg agcggcagca aatatacgat
      721 gcgacttgga ttggggacac gggcaatcac aggaatatca tctgaagatc cgcaagctgc
      781 gccagctcct gggagtgtct cagtggaaac aacaccttta gagacttcag cagggttctt
      841 ggaaaaagaa acaggttctt ataatatatt cggaaacggt catgggaatc tgtttccggc
      901 cacgaactcg attactaaac tggacgtaga gatgaaaaaa acgaatactg gtttagaggc
      961 atattattac gatcctgaaa caggagaaga aatggcttct gacatcatgt atgactggga
     1021 aaaattatac gcttcagatg aatcggttat ttatgcagga tttgctgcag caaggaatat
     1081 gacgatcaag gtagaaggaa tcgaagttgc ttactcagat cctgcgacag atcctcctgg
     1141 gcaagaaaga cctactcgct tgattgatcc gatttacact gtgacctcta gtaatcattc
     1201 aggaaaccaa gatcatacta tgtcctttag agcaaatgct gacggattat tgacaattaa
     1261 aaataaagca acaggagagg tgcttaagaa cgcaaaaaac ttggctatca ctgcgaatcg
     1321 ggaatttgat tatcagacaa aactgatgga aggaacgaac gaatttactt tcagttttac
     1381 gccagataaa aactatccgc cagaagaata cgtagagatg actagttatg aaacaaaaga
     1441 gattcttcat acagtggact accgtaaacc taaatattcg acagtttatg taacaccaga
     1501 agggtcagat gcaggaaacg gaagcagaca aaatccatta tctctgacac aagcattgag
     1561 atacgcatat gctgggcaga caatcgtcat ggcgcctgga acttattcct tcgaaagagg
     1621 attgacgatt ccaaaaggcg ttaacgggac aagagaaaac ccgattcagt tgattgcaga
     1681 gaaaaatcca gaaaacaagc gtcctgtact tgattttaaa ggaactggaa atgggatgac
     1741 actgttcagt cactactggg gtttacgagg gtttgacatt acaaatagtg cagctatgca
     1801 aaaaggatta cagatttctg gaaatcataa tttgatcgaa gagattcatg cttatcggaa
     1861 tggaaataca ggaatccaaa tcagcggtag ctcaaatgat cctttcgaag catggcccgc
     1921 gcataactta gtcaagaact gtacatcttt tgaaaatgcc gatccaggat ttgaagatgc
     1981 cgatggattt gcggccaaac tgacaatagg agaaggaaat gtatttgacg gttgtatcgc
     2041 gtatcataat gcagatgacg gttgggacct ttttgctaaa aatgaaacag gttcgatcgg
     2101 aaaagtaatc atcaaaaact cagttgctta tcgaaacggc tgggtaccag gaattgaggg
     2161 agaaggtaac ggtaatggat tcaaaatggg cggttcttct ttaacgggcc ctcatgaatt
     2221 gatcaatagt ttgtctttcg aaaatcttgc gaaaggaatc gattccaata gcggaacaga
     2281 tattatcgtc aatagcagta caagttttaa taacggttca tataatgtgg ctctttatac
     2341 ctcttcagca ggaaacacag actaccatgc ttctggtatc ttatctttcc gaacagagaa
     2401 tcttgagatg agagagcaga tccgcccatt gaatcaaaac gaagatcaaa tctaccactc
     2461 gttgaactac tattggaatg aacgagagaa acaatcagaa aatactcttg gacataccat
     2521 atcaaaagat tggatcgagt ctctaaatac atgttcgaaa gaaggtcagc agcctgttac
     2581 gcgctatgaa aatggaagta tccatgttca tggactgatg cagattaaac cggggaatcg
     2641 tcaaacagaa aaggaaagag tggatggtct tccattatct gtaggtgcta tttttgacgg
     2701 agaaacctca ccgtcttcgg aatatccaga gtatgcaatc gtacaagaac caaatcagac
     2761 aaaaccaaat gaacaaaata atccgattat tatacaagaa gcaaatggtg tggcggagaa
     2821 tgcagaagga aatatcattg aactgaagcc ttttacagtt tttgtgtcaa ctttcacacc
     2881 aaatcaagga acgcctgtct ttgaagatat gcgttatttc ccagaaaaga tccaaatcgt
     2941 tgaaacacag tcaagtgctc aattttctaa tcataccgct attttttcag caccttcaaa
     3001 aggagaatat acgctagaag tcacttatcg ttcggaaata tttgatgggg aaaaatggtc
     3061 tgctcataca gaatctgtta cccagcaaaa agcgatcact gtgcgagaaa ctgccgatgt
     3121 gcccccggga aatgacaaac cagaaagaga aaatccagga aatgaatcac caaatggaaa
     3181 accaaatgga aaaccttctc atccggatag gacagcaaaa gaaagtgaaa aaaatggcac
     3241 tcaaggactc ccaaaaacgg gagaatttaa taacccgttg ctagcattag ctggaggaat
     3301 tttgttaatc ggtgtagttg tttatgtcat gaaacagcga aaaaaataag tggaatttct
     3361 ccttttatga tgtaaaacaa ttgactgagg aaagtaaaaa tagaaggaaa tgagaccaat
     3421 agatgtctca cttccttcta tctttttata gatgaggttc tccttgctgc ttttgattgg
     3481 ctgttcttat tacttgatgc aggacagctt tttcacaatc tctccactaa acggtgttca
     3541 atcagctgac cctcggtatt agctcaacaa acggcaaaat atgaggaact atttttccgt
     3601 ttttttctct aatactcagg tcaggacgct gatttcacaa cctctcgata aacggtgttc
     3661 aaaagccagt ttctcggtag taagtcaaat ttatgcaaaa atgtggaaag ccattttcac
     3721 aaaatttttc ttactacttg aaactaagca gctttttcac aacctctaat acatggtatt
     3781 ccagaagtaa cttcttcgaa aaataagccg aaatgtcaca aaaatttgag aagcgatttt
     3841 cccaaattcc ttcttatttc ttgaagctga ccacttctgg cacaacctct taccagattc
     3901 gcactctttt ttctggcgct agatacatag cgtcttgttc cgtgatacca aatgtttcat
     3961 agaattcgtc aaggtttttt ggttgcacgt ttgctcggag tttagcaggg gcatggacat
     4021 cgatttgcag taaaagttgt tggtattctt tttttgcttt tgtacgccaa atcgttgccc
     4081 aattgacaaa gaattcctcg atagaaagat ctgattccgt ttttgctgct tccaatgcac
     4141 agcttagacc gcctgcatca gcaatatttt ccgaaacggt taattttccg tttacttttc
     4201 cgtcagcaaa aggcagacca tcgaattctt cgatcattgc ttgtgctttg ttttgaaatt
     4261 gtgtaagatc ttcttctgtc caccagttgt tcaagttgcc atattcgtca aataaagcgc
     4321 cgttattatc aaatgcatgg gaaatctcat gagcgataac agctccgatt ccaccataat
     4381 tagcagagct tgattgttct agactgtaaa atggtgcttg aaggatcgct gccggaaaga
     4441 cgatcacatt ccgaaatgga tgataataag cattgaccgt gtcagcactc atttcccatt
     4501 ctgaacgatc cactggtttg ttccatttgc tgaaacgatc ttttaatgtg agtttgctga
     4561 aaacaagagt atttgaaagc aaagtaccac cctgatctgc aggtactgtt ttgaactggt
     4621 cgaataatgc tgggatctta tcggggtagc ctacttggat acctaagtta tttaatttta
     4681 tgacggcttt ttcttttgtt gcatccccaa gccagtcatt ttcttctaga cgttttttat
     4741 aaacggcgat catttttttc accatttgtt ctacgtcttt tttagcagct tcgccaaaat
     4801 atttttttcc gtagtaatcg ccaactactt gatcgaattg tccagaggca aggtaatacg
     4861 ctgctttttt ctgaggcatt gcttcgtctg ttccagacaa tacgcgagaa tagattccgc
     4921 tgacttggcg gaattcttct gatagataac cactaagaga acgtactgtt ttaatgatca
     4981 tccagctttt gaataacgga aaatgagctt ctgtcaggat gtcattcaaa tgatcgaagt
     5041 acactggatc agttacgatc actttatctg gagttgtccc aatcaaaccc gtgacaagtt
     5101 ttttcaagtc cagtttatct gtgtatttaa taaatgtatc aaaagattgc ggattataca
     5161 ttttgctgta atctgcgttt tcttctgcac tttttacatg aggagcaatc aatttatcaa
     5221 attgcactgc ttgttccgtg attgcttctg cctgactttt ttctttgccg gcaagctcta
     5281 ataactgaac agccatatca aagaaaacgc ctattagttg tgcaccgttg ggatggtctg
     5341 atgcataata cgtcttatct ggaaggaata aagaaggcgg ataagcaaag aaggcgttcg
     5401 tttgcgcatt cttcatatct gcatctacat ccagattaaa tggcaatggt aatgtatcca
     5461 acagccattc tggaaattgc ttattcaatt cgtcgaaaga ctgcaatgat tcaacttttt
     5521 cgattatagg ttgaagcggt tgagcatgca actcatttct tcggtcataa tcgtttgcca
     5581 gttgataata tgcaaggaaa tgtttcattt tttctgaaga cactttttct ggatgggcaa
     5641 gcagatcgtc tgtttctccc atcaaaagtt ggtcgatccc gtcaactaaa tcttggaaac
     5701 cgcctgtagc tggtttatct gcagggattt ttgctgtttt ttgccattct tcattgactg
     5761 cttcatagaa atcttgtttc aatcgttctt tgttcattca tcgatcgctc cttttttgtt
     5821 tcgtgtgccc attatcttac aaaatgaagg aattaacaaa tagaacataa aaaattgaaa
     5881 attcgaacaa tcaaactgat ttgttttttt caatccttca cttataatga aaaaaaggag
     5941 gcgatcccaa ctgaatcaag tagtggataa acctttatat ttcaaagtag ataaaaaaat
     6001 taaaaaatta gccagcactc agcagctgca gtcaagaaaa agtaaattgc tttttttagc
     6061 tctggttttt gaagatcaaa gttatgtgat tatcgatcaa tcaggacatc cggtagaata
     6121 ttctccagca aaatatactt atcaagaggg tctttcttgt aaaaaatgga agctgatcaa
     6181 tgaagagcca atcgagttat ctagatggat caacagaaaa gaagatattc ccgttttgat
     6241 cgaagaaaaa aggtcaggaa aggaactcgc aaattgctgg gtgggattgc cagaagaacg
     6301 ttttttgagg tataagaaat gggctacccc ctcaggctat ctttgtggta cttacgcggc
     6361 agcagttctc cttgcctatt atcaagacta tcgtaagggg tggatgcttc caaatgagat
     6421 tcgcaagaaa aatacggctg attcgttggt tttgacgaag gcattgcgta gccagatcca
     6481 accactagga cttcctacga ttccttttca ggttagtaca gggatttcta gttttttaaa
     6541 gaagaatggc aatcatgagc gtgcacgagc aactcttcta ggttcatggc agcgagctac
     6601 aaaacgtata cgtgaaggca aacctgtgat gattggtatt ttgaaagtac ttggcagtac
     6661 atatggcaat cattgggtga cagcgtacgc ttattttgaa actgaaacag gagaacgtta
     6721 ttacaaagta cacgacaact ggggcgatta tcataaagtc attcctgcca gttggagtaa
     6781 cggaacagtt tcacttcctt aagaatttgc tgaaattaac agaaaatctt ggaaaaatat
     6841 gttaaactat cgaagaatag agaagttttg gggaggacta catgagtaat aagtatcagg
     6901 atgacacgtt gaagattttt agtttgaatg gcaaccgtcc attagctgaa aaaatcgcga
     6961 aagtgttcgg aacagaatta ggtaaaagcg tcgtgaagca atttagtgat ggcgagattt
     7021 ccatcaatat cgaagaaagt attcgagggg atcatgtgta tatcgttcaa tcaacgaatg
     7081 cacctgtcaa tgattactat atggaactac tgatcatgat cgatgcaatg aagcgagcaa
     7141 gtgcgaaaac gatcaatgtc gtattgcctt actatgggta tgctaggcaa gaccgcacgg
     7201 cgaaacctca tgaaccgatc actgcgaaat tgatcgctaa cttgatcgaa gaagcagggg
     7261 caactcgagt actgacttta gatctgcaca cggttcaagt acaaggattc tttgatattc
     7321 ctgtagataa tttatttacg atgccgttgt ttgctcatta ttaccgtcaa ttaggtctga
     7381 cgggagatga catcgtagtt gtttcgccaa aaaacagcgg tgttcagcgt gcacgtagtc
     7441 tttcagaata tttgaacagc acattagcca tcgtagatca tgcagatgat gcttcaactg
     7501 agggtggtta tgtgatcggt gatgttcaag gaaagacatg tatcatggta gatgatatct
     7561 tgaacacagg agcaacgctt gcacgagcag caaatgtatt aaaagaaaac ggcgcaaaag
     7621 aagtatatgc ctgtgcgtca catggattat tgtccgatca tgcgaaaagt attttagaca
     7681 atgcaccaat caaagatatt tgtatcactg attctgtttt cacagaagaa aatcgtcatc
     7741 cagaaaactt aacaatcgtt acatgttcag aattaatggg tgaagcggtc aaacgcatcc
     7801 atgaaaatac accgatgagt ccgttattcc gtctggaaga aaaagaattc gaataaacaa
     7861 aaaaaagagg gaaagaagtt gtgacagaag tgagcagctt caaggagctg cgacaagact
     7921 tttgtcacag ctcctttttt ctatgcccat gaaacgtcct ctagacaaaa atgagctctt
     7981 ctttggtttg ttcgctattt tatgctagaa tgtgcctagc aataatttgg aagggatgaa
     8041 tgaagtgaaa actatttatc tggatcatgc ggcaactacc cccatgcatc cacaagtaat
     8101 tgaagcgatg acggcaatca tgcaagacac gtttggaaat ccttcaagca tacatggatt
     8161 tggtcgccac gcgcatgaaa aattggaaac agcaagacaa atcattgcag acagtctgca
     8221 agcaaaacct cacgagatca tctttaacag tgggggaaca gaaggggata atacagctat
     8281 cttagaaaca gcgttctcgc gtcaaaaaga aggcagacat ctaatcacta cagctatcga
     8341 acatcctgct gttcttaata ccatgaaata tttagagaca aaggggtttg acgttacata
     8401 tctgcctgta gatgaaaaag ggaatctgtc gatcgaagag gtcaaaaaag cgttacgaga
     8461 agatacgatc ttggtctctg tgatgtacgg gaacaatgaa atcggaaatt tgatgccaat
     8521 caaagaaatc ggtgaacttt tagcggatca tccagcttat ttccatacag atgcggtaca
     8581 agcgtacgga aaccaaacga tccatccaga agaattagga atcgatttat taagtatctc
     8641 tgctcataaa atcaatgggc caaaaggcgt cggtttctta tataaacgag acggagtaaa
     8701 tatccctgca ttgcttttag gcggggagca ggaagaaaag cggcgtgcag gtacagaaaa
     8761 tttagcaggg atctggggta tggcaaaagc agttgaactg ctgtcacctg aagaacgtga
     8821 agcacgtaac aacaaatata aaggattcca gcaattgatt cttgatacat tagaagcaaa
     8881 cgaaatccct tatcaagtaa atggtgattt aacgaataaa ctccctcatg tgttgaatct
     8941 tcgcttaatt ggcgaagcaa atgatttgct actgatgaag cttgatctta atggggtcgc
     9001 tatttctact ggttcagctt gtacagcagg aaatatcgag ccttcacacg tattagaagc
     9061 aatgtatgga gaagatacac cgatactaaa agaatccatc cgtatcagct ttggtttggg
     9121 aaataccgaa gacgacatcc agtatttcct tgatcaatta gttcgtgctg tcaaaaaata
     9181 actagtgaag tcattcttat atgaacagat gtctgagaga acgccttcat tttttaggga
     9241 acttaactga aaaacctagt aaaaatcagg ataaactttt ataataaaga gaaactaatt
     9301 ttatcgaggt gaaaatcatg gcatttgaaa caacagcaac ggtactgggc gcaccaatta
     9361 gctatcgttt gcatcctgat gcaaaaagat atacattgcg cgataatgga tttacagaaa
     9421 caaacggagg caattatcaa ttgatccgac cattagatgc cacgccgcaa agcaaagaag
     9481 gattcaaatt aaaaatcact gtatcaaaag atattcaaac attgaaaatg tcgatcacta
     9541 cagcaaatgg attgaaagca gtcaatattt tcaaagatcc aaaacagaaa atgagccaag
     9601 ataaatttta ttttctgatg gatgggatga ttagccgtgg gttattcgaa aaagtcgaat
     9661 gatctgtgga tataaaaaga caactagttt tgaaacggcc tattgttaga tgactaac
//
LOCUS       DAYYHS010000098         9544 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000098 DAYYHS010000000
VERSION     DAYYHS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.099"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..977
                     /gene="gnd"
                     /locus_tag="ACHA6A_002515"
     CDS             75..977
                     /gene="gnd"
                     /locus_tag="ACHA6A_002515"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8970650.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            979..1980
                     /locus_tag="ACHA6A_002516"
     CDS             979..1980
                     /locus_tag="ACHA6A_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8970651.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1977..2261
                     /locus_tag="ACHA6A_002517"
     CDS             1977..2261
                     /locus_tag="ACHA6A_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8970652.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2294..3091
                     /locus_tag="ACHA6A_002518"
     CDS             2294..3091
                     /locus_tag="ACHA6A_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8970653.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3105..3938
                     /locus_tag="ACHA6A_002519"
     CDS             3105..3938
                     /locus_tag="ACHA6A_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8970654.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3956..4813
                     /locus_tag="ACHA6A_002520"
     CDS             3956..4813
                     /locus_tag="ACHA6A_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8970655.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4835..5344
                     /locus_tag="ACHA6A_002521"
     CDS             4835..5344
                     /locus_tag="ACHA6A_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8970656.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5381..5782
                     /locus_tag="ACHA6A_002522"
     CDS             5381..5782
                     /locus_tag="ACHA6A_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970657.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5894..6247
                     /locus_tag="ACHA6A_002523"
     CDS             5894..6247
                     /locus_tag="ACHA6A_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970658.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6281..6724
                     /locus_tag="ACHA6A_002524"
     CDS             6281..6724
                     /locus_tag="ACHA6A_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8970659.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6741..7826
                     /gene="uxuA"
                     /locus_tag="ACHA6A_002525"
     CDS             6741..7826
                     /gene="uxuA"
                     /locus_tag="ACHA6A_002525"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8970660.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7819..8532
                     /locus_tag="ACHA6A_002526"
     CDS             7819..8532
                     /locus_tag="ACHA6A_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8970661.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8739..8888)
                     /locus_tag="ACHA6A_002527"
     CDS             complement(8739..8888)
                     /locus_tag="ACHA6A_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970662.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9284..9484
                     /locus_tag="ACHA6A_002528"
     CDS             9284..9484
                     /locus_tag="ACHA6A_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8970663.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3210 a         1497 c         2114 g         2723 t
ORIGIN      
        1 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
       61 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
      121 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
      181 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
      241 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
      301 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
      361 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
      421 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
      481 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
      541 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
      601 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
      661 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
      721 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
      781 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
      841 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
      901 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
      961 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
     1021 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
     1081 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
     1141 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
     1201 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
     1261 caccagacaa tcgtatctat catacctgct ttaattgaac gaaatgctgt agcagaaaat
     1321 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
     1381 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
     1441 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
     1501 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
     1561 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
     1621 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
     1681 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
     1741 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
     1801 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
     1861 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
     1921 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
     1981 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
     2041 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
     2101 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
     2161 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
     2221 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
     2281 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
     2341 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
     2401 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
     2461 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
     2521 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
     2581 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
     2641 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
     2701 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc
     2761 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
     2821 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
     2881 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
     2941 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
     3001 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
     3061 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
     3121 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
     3181 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
     3241 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
     3301 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
     3361 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
     3421 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
     3481 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
     3541 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
     3601 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
     3661 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
     3721 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
     3781 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
     3841 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
     3901 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
     3961 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
     4021 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
     4081 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
     4141 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
     4201 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
     4261 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
     4321 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
     4381 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
     4441 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
     4501 ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc
     4561 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
     4621 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
     4681 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
     4741 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
     4801 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
     4861 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
     4921 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
     4981 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
     5041 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
     5101 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
     5161 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
     5221 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
     5281 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
     5341 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
     5401 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
     5461 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
     5521 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
     5581 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
     5641 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
     5701 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
     5761 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
     5821 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
     5881 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
     5941 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
     6001 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
     6061 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
     6121 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
     6181 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
     6241 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
     6301 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
     6361 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
     6421 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
     6481 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
     6541 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
     6601 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
     6661 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
     6721 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
     6781 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
     6841 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
     6901 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
     6961 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
     7021 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
     7081 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
     7141 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
     7201 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
     7261 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
     7321 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
     7381 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
     7441 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
     7501 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
     7561 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
     7621 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
     7681 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
     7741 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
     7801 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
     7861 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
     7921 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
     7981 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
     8041 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
     8101 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
     8161 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
     8221 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
     8281 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
     8341 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
     8401 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
     8461 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
     8521 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
     8581 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
     8641 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
     8701 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
     8761 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
     8821 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
     8881 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
     8941 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
     9001 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
     9061 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
     9121 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
     9181 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
     9241 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
     9301 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
     9361 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
     9421 agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc
     9481 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa
     9541 aaat
//
LOCUS       DAYYHS010000099         9452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000099 DAYYHS010000000
VERSION     DAYYHS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.100"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..851)
                     /locus_tag="ACHA6A_002529"
     CDS             complement(183..851)
                     /locus_tag="ACHA6A_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8970664.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            985..1272
                     /locus_tag="ACHA6A_002530"
     CDS             985..1272
                     /locus_tag="ACHA6A_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970665.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1688..3226
                     /locus_tag="ACHA6A_002531"
     CDS             1688..3226
                     /locus_tag="ACHA6A_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8970666.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3223..4188
                     /locus_tag="ACHA6A_002532"
     CDS             3223..4188
                     /locus_tag="ACHA6A_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970667.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4175..5497
                     /locus_tag="ACHA6A_002533"
     CDS             4175..5497
                     /locus_tag="ACHA6A_002533"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8970668.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5560..5664
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5738..6481
                     /locus_tag="ACHA6A_002534"
     CDS             5738..6481
                     /locus_tag="ACHA6A_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8970669.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6496..7488
                     /locus_tag="ACHA6A_002535"
     CDS             6496..7488
                     /locus_tag="ACHA6A_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8970670.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7500..8237
                     /locus_tag="ACHA6A_002536"
     CDS             7500..8237
                     /locus_tag="ACHA6A_002536"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970671.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8227..8949
                     /locus_tag="ACHA6A_002537"
     CDS             8227..8949
                     /locus_tag="ACHA6A_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8970672.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9033..>9452)
                     /locus_tag="ACHA6A_002538"
     CDS             complement(9033..>9452)
                     /locus_tag="ACHA6A_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8970673.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3109 a         1619 c         1982 g         2742 t
ORIGIN      
        1 tttaagcgaa ttttttctcc caataagtaa gaaaataatg gcaaaataaa aaaacaaact
       61 attttcgcag atcgtttgca aaaatagttt atttttttat aaagaagatt ttactgaata
      121 atatcatttt agtttacata atgatatttc tgtaaattaa ataaagtagt gaaaaataag
      181 aatcagtgct ctaactgttc aagaggatca agtggttcaa atgtttctgt agtctgtttt
      241 gcttccaatt ggggcaaaac agatccaatt tccagatact tcactttatt caatgtataa
      301 gatagaatag aacagctaag taatgtatac agaaaatgtg tgagatccaa ataaagcaag
      361 gagacaacta aaataccaaa agtaatcaag aaaagacttt tgccaatctt aatatgaaaa
      421 tatttttcaa tggcttgata aatgatatct gttccgcctg tgcttccgcc gaatcgaaag
      481 acgagaccgc aaccaatgcc agaaagaata cctgtcatca aactattgat cagtagacta
      541 tctacagcaa aatgtatatg aaggctctcc cataaatgta aaaagaaaac tagcgcaaat
      601 gttccgacga tcgtccggag aaaagcgtgt tttccaaaaa gaaacaatga gataagcaat
      661 aaaggtaaat taaaaagcag tgaagtgaca gaaggagcga atccaaaaag attatgcaaa
      721 agcaaagcaa taccagtaac cccaccatct gccagcttag atggaatatt gattcctgtc
      781 acagctaatc cgtagattcc tgcgcctaaa atgattccgc acaattctgt aaataaattt
      841 aattttttca tttctagcct cttttctttt gaaaattttc agcaacttct ttattatact
      901 gacttatact cttacttcaa gaggaaaatg aaaaaaaatt atttttggct gtttcttttt
      961 atttatcaaa taaggagtgg aaaaatgcga gcagcagact tattgacaat tcttacaaaa
     1021 tcacccaaaa aaacagagta tcaaggagtt tttttggctc aagaacaaaa actggtgcct
     1081 gtgacaatgg tccagataaa taaagcaaat caatttatct ttttatttga acacggcaaa
     1141 gcaccacttg ctatgaaaga catctttctt tttttgatga agaaccgtga gaaagctgtt
     1201 tattatcagt caaaagaaga actcattcct ctttatggag tgaaagagat agaaaataaa
     1261 gtggtgatat aagtaaaaat aagaaaacaa gacgtttatg atgtcaaaac aaccattttt
     1321 tacatttttg gcaaaaaagt gtcatgatac agtataatat tcgttgaaca ggccaataaa
     1381 ggtgttctcc cccacgagat ttctacttta ttggctgttt ctttttatta aaataaaagt
     1441 tgtgtgtgta aaaaagatat agctgattcc aagtgacaat ttcgtataga tttttatggt
     1501 attcgtagtg aaaaaacgag aaccaaaaag ttgatccgac tttgttgctt ggattctttt
     1561 tttgtcagca tatttagagg tatttttttc ttttttttgt tcgtgctgta aggttaaggc
     1621 aagacaagtt agttgggaaa aaggaaagaa atgtttatac ttaaagaata acagaaagga
     1681 aggtcgcatg aataatttta tacaaacatt aagtgaaaga aaaggcgagt tgctggaagc
     1741 tgcatttcaa catctgtcca tttctctcat ttctttgctg attgcagcac tgattgctat
     1801 tcctttagcg atttgggcag ccaatcataa aaaaatggca gaagtcttat tgcagatcac
     1861 gggtgtgctc caaacgattc cttcacttgc cttattaggt ttacttatcc catttgtagg
     1921 gatcggaact gttccagcat tgattgcact tgttatttac gctttgcttc cgattttcca
     1981 aaacacgtat attggtctag ctgaaattga cccgtctatt gaagaagcag cggtagcatt
     2041 tggtatgtcc agaatgagac gcttgctgaa agtagagctt cccatcgcat tacctgtcat
     2101 tatctcaggt atccgaactg ccttagtttt aatcatcggt acggctactt tggcagcatt
     2161 gatcggagca ggtggattag ggacatttat tttgttaggt attgaccgaa atacacctgt
     2221 tttgacattg attggtgcga tcagttctgc cttgcttgca attatattca gcggactgat
     2281 tcgctggttg cagcataaga aacctcgata ttccatcatt actttagcag cagtggtgtg
     2341 tttatttggc ggcgtttctt tatatcagtc tgccttgttt aaaaatgaaa ccatcacgat
     2401 tgctggaaaa ttaggagcgg aacctgaaat cctgatccaa atgtataaag aattgattga
     2461 agacaacaca aaaacagaag ttgaattaaa accgaatttt ggcaagacga gttttttgtt
     2521 caatgcactg gatcaaggtc aaatcgatat ctatcctgag ttcacaggta ctgtcctaga
     2581 aagcttggtg aaggtacctg aagcaatgaa aagtgctgat ttgacaaaag aaaaaacata
     2641 tgacgaagca gaagaattat tgaataagca attcgatatg acattattaa aaccaatggc
     2701 ttaccaaaat acgtatgcct tggcagtcaa gagatctttt gcgaacgaac atcaacttaa
     2761 aaagatttcc gatttggcag ctatctctga tcagatcaaa gcgggattca cactggaatt
     2821 tattgatcgt tcagatggat ataaaggaat ccaagaagca tatggcttga atttttcaag
     2881 tgtacaaagc atggagccaa gtttgcgtta ccaagcaatc aataatggag atgtcaatat
     2941 tgtggatgcc tattccacag atagccagct caaggagtac gatctagtga ctctagaaga
     3001 tgatcgaggg ttattcccag cttatcaagg cgcaccgctg atgaaaaaag attttgctaa
     3061 agaacatcct gaaatcgtta aagcactgaa taaattaagc ggaaaaataa cagaggatca
     3121 aatgattgat atgaattacc aagtcaatgt cgaaaagaaa aagccgacag aagtagcacg
     3181 aatcttcctg aaaaatgaag gattagtaaa ggaggaagat cagtgactaa attaatcgag
     3241 ttcaaacatg tgcaaaaaaa gtatgatgga aagtatgtca tcgatgacct aaatctggca
     3301 atcaaaaaag gagaaatctt tgttttggtt ggtccttccg gtagtggaaa aacaactact
     3361 ttgaaaatga tcaatggatt atctaaacca tcagcaggag acatctactt caaaggaaaa
     3421 tcgttaaatg aatataatct gcagaaaatg cggtggaata tgggatatgt gctgcagcaa
     3481 atcgctttgt tcccgaccat gacagtcaag caaaatattg aagtcatccc tgaaatgtta
     3541 ggttgggaaa agcagaaaag agcagaccgt gtagatgaac ttcttcaaaa agttggctta
     3601 tctcctgata tttatcgaga tcgtatgcct agagagttgt ctggaggaga acagcaacgg
     3661 atcgggatca tccgagctat tgctgcaagc ccagatgtga ttttgatgga cgagccattt
     3721 agcgcattag atccgatttc aagaaactca ttacaagaat tggttttgtc cttacacgaa
     3781 gaattgggca cgaccatcgt tttcgttacg cacaatatgg aagaagcaat caagctaggt
     3841 gatcgtattg catttatgaa ggatggagaa attatccaat gtgacacacc agagcagtta
     3901 ttgatgaatc ctaaaaacga ctacgttcgg catttctttg atgaaccgaa acagacgaaa
     3961 gagtggcgag tagaagattt agtcgtcaat ggatatttct taaatgaaat accagaaaat
     4021 gctcgtcaac aagtttgctt tgatacgcct atgaaagaag tatattcgct tctttcggtg
     4081 tatccaagca ttgtgatagt agaaaagcaa agaagtattg ggtcgctgac aagtaaagaa
     4141 atctttgctt ttttaagtcg agaggaggaa ggaaatgaaa acatatgatg caatcatcat
     4201 cggttcaggt gtgagcggct taagtgctgc ttatggattg aaagaagcag gtaaaacagt
     4261 actagtggtc gaagaagatc tatggggcgg gacttgtcct aaccgtggat gtgatcctaa
     4321 aaaagtcttg ttaagtgcag tagaggcacg taatcgggta aaacaattga gtggaaaagg
     4381 ttttaacgag atcccaactg ccaactggga agaacttcaa aaattcaaac gaacatttac
     4441 ggatccagtc ccagaaagcc gaaaaaaaca attagcagaa gctgaaatcg atcatctttc
     4501 aggaaccgct cgttttttgg atgattcatc tattgaagtc aacgaagaag tcttccatgc
     4561 ggattactta gttttggcaa ccgggcaaag acctacgatc ttaccagtgg agggaaagga
     4621 atacttgaaa actagtgcag actttttatc attgcctgta ctgcctaaag aaatcatttt
     4681 tatcggtggt ggttatattg cttttgagct agctacgatt gccaatgctg cagggagcaa
     4741 agtaaccatt gtccatcata atcaacggcc attaaaagaa tttgaggcgt ctttggtaga
     4801 ggaagccgtc catcaaatgg aagcatctgg cattcagttt gcttttggtg ttgaaaccca
     4861 aaagatcatt tcagaaggta ctcgctatcg tttagtcgga aaagagacgg aattagtagc
     4921 agatatgatt ttttgtgcga ctgggagaca gccgaataca gaatctttag cacttgagca
     4981 agcgaatatc gtctttgata aacacggaat tgcagtcaat gattatctgc aaacgagcaa
     5041 tccaaaaatt tttgcgtgtg gagatattgt ttcaaggaaa accccaaaat tgacaccagt
     5101 cgccactttt gaaggaaatt atgtagctaa gcggataacg gatgcaacaa gcgaacctat
     5161 caaatatccg attataccta cgatcgtcta cgccagtcct aaacttgctg aagtaggagt
     5221 tacaaaaagt cacgcctctt cgtcagatca agtggtagag atggatctta ccagttggtt
     5281 cacctatcat cgtgtcaatg aaccagttgc taaggctgaa ttgacctttg accagcaaaa
     5341 ttatttgatt ggtgctgcag tgattagcga gcaagcagac gaattgatcg atgatttgac
     5401 actcgtcatc aatcaaaaat tgactaaaaa agagttggat agctatatca tggggtatcc
     5461 gacattggca agtgatctct cctatttatt gaaataatca gcatgataac taaagcgctt
     5521 gttgcttttt ccttcttgtg ctacacttta aaaaatgaat acctgtttct ggggatgtcg
     5581 tagacgacat gagagtaagt tgtgaactta gacccattga acctgtcagt taatactggt
     5641 gaagggagaa acatagcaga cgcttgaagc gggttactat gtcgtgccca tttcaagcgt
     5701 tttttgttat tcatattaga agaaaggagg taacaaaatg aagaaaaatc tttatccagc
     5761 agtcagtatg gcattcattt taattatttg gcagattgtt gtcatagttt ggaaaatacc
     5821 tgcctttatt ttacccggac ctgctgctgt attttctgca ttagcggcag atgcacaagt
     5881 attactttcc cattctgtca cgacattaaa tgaagcagtg cttggtttgg taatagctag
     5941 cgttctttct tttattactg ccttggcaat ggattattgg aagtttttag aaatgtcttt
     6001 ttatccgtta ctagtgattt cacaaatgtt gccaatcatg gtgttagggc cattacttac
     6061 cctatggttt ggtttcggta tgcttccaaa agtgatcctt gttgtcttga taagctattt
     6121 tccgatcgta gtttcttttt cggatacatt gaggaaagta tcaaaagagc aaatcatctt
     6181 tttgaaaacg atgggggcag atacagggaa gatttatcga atatataaaa taccagcggg
     6241 tttatccggc ttcttttccg gtttaaaagt ggctgctact tattgtgtca gcggagcagt
     6301 cgttggagaa tggctaagcg ctcaagcagg cttagggtat tacatgatac gcgtaaaaaa
     6361 cagctaccag atcgataaag tttttgctgc gatcatttgt gtcattctat tgagtttatt
     6421 gctgaatggc gcttgttcat tattgaaaaa agggtattat cgtatgcttt atatttttta
     6481 agagaagggg atcaaatgaa aaaattatgg cttttattac cgctactgtt gcttttatcc
     6541 gcttgtggca ctgcaaaaga aacaagtagc aaacaagaaa taaaggattt gaaaaaagtg
     6601 acacttgttt tggattatgt accgaatact aatcatactg gcatctacct agcaaaagaa
     6661 aaaggctatt ataaagaggc tggtttaaat gtccaaatca tcgaaccagg agataattcg
     6721 acaagtattg gtctagtagg tgcggacaaa gcacaatttg gtgtaagtta tcaagaagat
     6781 gtcacttatg ctcacgcaga tggacagaat ataccagtca aagcaattgc tacagtgatc
     6841 aaacataata cttctggatt cgccacccta tcagacagta acatccattc accaaaagat
     6901 tttgaaggaa aaacatatgc aggttggcaa agtccaagtg aagaagctgt cttaaaagct
     6961 gtgatggaaa aagacggcgg ggacttttca aaactgacaa tggtaggaag caatggagaa
     7021 gggccagaaa gcttaggaaa atcctcagat atccaatggt actttgaagg ttgggatatg
     7081 atcaaagcaa aagaagcggg tattgaacta aactatatcc cattgaaaga attagatgaa
     7141 cggttagatt actatactcc ggtaattatt acgaatgatc aactgatcaa aagtgatcca
     7201 gaattggtac agtcttttat ggatgccacg aaaaaaggct atcaggaagc tattaaggat
     7261 ccaaatgaca gtgctaaact gttgcaaaaa tatgcaaaag aaaatgatcg gacgttttta
     7321 gaagaatcac aagcattctt gtcaaaaaat tatacggatg atcctgagaa ttggggattg
     7381 atggaagaaa aagtttggcg caattataca gcttttatgc aggaaaacgg attgatcaag
     7441 caagatgtac cttcgcaaga actattcact aatcagttta taaaatagga gtgatagata
     7501 tgtcaatcca aatcagccac ttgaatgtag tcattgaaaa cctgaaaata ttagaagaca
     7561 tccatttcac tatccctaaa gataccttta catcgattgt cgcgccaagc ggagccggta
     7621 agtcaacatt gctgaaaact ttaacaggag tccagcctat gacttctgga acgatcaaag
     7681 tagacgatca aaaagtaact ggtctccata cagcatttag ttatatgccg caagaagata
     7741 tgttacttcc ttggctgagc gtctatcaaa acgttacgtt atatcaaaaa atcaatcatc
     7801 taacaatcaa tgaagaacaa gtaagggatt atctggatat ctttgggtta aacggctatg
     7861 aacatttttt gccggaacaa ttatctggcg ggatgaagca gcgaacggcc ttgttacgaa
     7921 caatcatgaa tccttcttct tacttgcttc ttgatgaacc atttggagcg ttagatgcta
     7981 tgaccagagg gcagatgcaa gactggatgt tgaagctgcc taaaaaagca aagcggacta
     8041 ctttacttgt gacgcatgat atcgaagaag caatttattt atcagacagg attcttgtgc
     8101 tttccgcccg tccggcacat gtgattgctg agattcaagt gcctgaaaaa cgacggtcac
     8161 gtgaatggct actgcagcaa tcagagttga agcaaacgat ttatcagtta ttggcaggtg
     8221 aattagatgt taagtgatgc tcattgtcat ttagatggga gtcagagttt agcactcctt
     8281 cagcaggaac acacgatttt gacgatcatc aattgtgatt cgccagaaga atggaaggaa
     8341 aacagacagc ttgcagcaag taagactcaa gccttaagtt acggtatcca tccttggaaa
     8401 gcagatagct atacgtttga acaagttgag ccttttttga aaaaagcaag gatcatcggt
     8461 gaaatagggt tagataatat ttggacgaat gtaccgatga ctacacaaaa aaaagtattt
     8521 gagcgacagc ttgcttttgc agccataaat gagaaacctg ttgtactgca cacaaaagga
     8581 tgcgaaaaag agatcttaaa ttatatccaa gcttacccta atcactattt gatccattgg
     8641 tattcttctt tggactatca acaagactat attgatttag gctgttattt cagcattggt
     8701 cttgatttga aaaagaatcc agcagtctgg caactagcta aagcagtccc aatcgaccac
     8761 ttacttattg aaacggatgg actacatgca gcagagtggg ctttaggggg gattttttca
     8821 gaaaaagatt atgtctcact gcttgaagaa cagttaaaga caatcgctga gatcaaagag
     8881 ctatcagttg aagaagttaa aaaaaatact tatcaaaact tggaacgttt cattagatta
     8941 ggtaaataaa aaagagatgt gacacaacta gtgtcgcacc tctttttggc tttctttgta
     9001 taaaagtaaa ctgtttgatt aataaatgaa tcttagtacc aaccgtttgc ttcccagaat
     9061 gctttcgccg cttcccaaga accataacgt gaagtaacat attgttctgc tactctttct
     9121 tggtttgcag cagagtagtc accatttaag taagaagaat ctaattggta acgtccgatg
     9181 taacgtccgt tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat ccattctttt
     9241 gcagagcttg tgcttgctgg agtagcagca gtttctgtta cagcaggagc aggttgtgtt
     9301 tcagcaacag gcgctgcttg tttttccgtt tgaaccgttt cttgtacaac aggttgttca
     9361 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
     9421 tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DAYYHS010000100         9384 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000100 DAYYHS010000000
VERSION     DAYYHS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.101"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..254
                     /locus_tag="ACHA6A_002539"
     CDS             <1..254
                     /locus_tag="ACHA6A_002539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970674.1"
                     /translation="CIKKYRKDTLKSFRINLPNKFIGGFKMSHTLLIKDTLNILDLNI
                     NIHFEENCLTKEKYKGQICLIYKGTLQYTPEEYPHLPIY"
     gene            314..547
                     /locus_tag="ACHA6A_002540"
                     /pseudo
     CDS             314..547
                     /locus_tag="ACHA6A_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(595..714)
                     /locus_tag="ACHA6A_002541"
                     /pseudo
     CDS             complement(595..714)
                     /locus_tag="ACHA6A_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
     gene            complement(1534..1677)
                     /locus_tag="ACHA6A_002542"
     CDS             complement(1534..1677)
                     /locus_tag="ACHA6A_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970675.1"
                     /translation="MENAITVITNSFFSSIKYLKTLFVCQADQCSFRLGRTLISSQVM
                     IEV"
     gene            complement(1930..3096)
                     /locus_tag="ACHA6A_002543"
     CDS             complement(1930..3096)
                     /locus_tag="ACHA6A_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8970676.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            complement(3184..3612)
                     /locus_tag="ACHA6A_002544"
     CDS             complement(3184..3612)
                     /locus_tag="ACHA6A_002544"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFM8970677.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            complement(3698..5122)
                     /locus_tag="ACHA6A_002545"
     CDS             complement(3698..5122)
                     /locus_tag="ACHA6A_002545"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8970678.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            complement(5490..6500)
                     /locus_tag="ACHA6A_002546"
     CDS             complement(5490..6500)
                     /locus_tag="ACHA6A_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFM8970679.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            complement(6752..7690)
                     /locus_tag="ACHA6A_002547"
     CDS             complement(6752..7690)
                     /locus_tag="ACHA6A_002547"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM8970680.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            complement(7727..8818)
                     /locus_tag="ACHA6A_002548"
     CDS             complement(7727..8818)
                     /locus_tag="ACHA6A_002548"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM8970681.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSINMLASTS
                     IVFLLISNNFIRIIQNIFVLNYIIYASVLMSNYKRTKVKNYIYLLAYFFFIIVCLIFI
                     NIRGTAFVNGILPLFRENIIFKAVYYFFE"
     gene            complement(8790..>9384)
                     /locus_tag="ACHA6A_002549"
     CDS             complement(8790..>9384)
                     /locus_tag="ACHA6A_002549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970682.1"
                     /translation="LEEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKK
                     HFASTSGSMKIAFANSSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYV
                     TNQVYFYHHVGDGENLKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMN
                     KRLLKFFIAIAFETDKLSRFRAAIRGVRDGYRGVIGD"
BASE COUNT         3271 a         1706 c         1215 g         3192 t
ORIGIN      
        1 tttgtataaa aaaatataga aaagacaccc tgaaatcctt tagaattaat ttgcccaaca
       61 aattcatagg aggattcaag atgtctcata ctctgcttat caaagatacc ctaaatattc
      121 tagacctaaa tatcaatatc catttcgagg aaaattgttt aactaaagaa aaatacaaag
      181 gacaaatttg cttgatttac aaagggactc ttcagtacac accagaagaa tatccacatc
      241 ttccaatata ttaaaagaac cttcttgaat cattgagtga aagaaaagag attcgtcact
      301 atttgaggac tgtttaacta gagacattag cgatctgcct gaaacctata caaccacttt
      361 acgaacgttt aaaaattttc tgccacagat tcaaaatgcg cttaactgca gctattcaaa
      421 cagaccattg gaatgtttaa acaaccacat caaagtatta aaacgcaata cgtatggctt
      481 tcgcagcttt tacaatttca aactacggat catgattcgt catggcaaaa catttttaac
      541 caaataaaca gaagaatgtg ggataaatct aaaaatcagt tttgctacag actctcgagt
      601 aaacggcggg agcagaagca actccttcgg aaataagccg aaattcacaa aaatttgaaa
      661 agcaattttc gtgaatttct tcttatttct cagagttaaa cacttctgtc ccagcctctt
      721 ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga gcttcttttt
      781 tgaatactat attcgttctg tttttcatct tcaaatacga actcatccgc agaaatattt
      841 gtcacaactc catgaatctt cttttcatta ccacctatat accacaggat tcctacacag
      901 aaacaacaaa aaaatagcat aaaataggtt ttctttattt tatatccctc cttttagatt
      961 aaaagctcac agaaaacgct ttctaaaaag acaatagaat aatcataatt tttcattttg
     1021 tattatttat acaaataata cgcatgttac tgaaaaatga tatggttccc tactttttga
     1081 gataagttaa ataaaatctg tcatcgataa aacagctagt acagtaatga taaatagtaa
     1141 gaacttaaaa tattttggat ttttattttt gaaataatcg aagaaaagta aaagtgataa
     1201 cgaaatagca aacaagatta tatctataag attattcatt gagaaatcgc cccatatgga
     1261 actctatttt gacctgacac accgagacat aaagcaatca tatcctctgc attgttttat
     1321 actattagaa ctatagctac acctattgga tgctactggc tctaagatta atgctaatac
     1381 agtaacatca atacctaaac tagcagaaac tctatttgca tcttcaattt cagatgcctg
     1441 gactgtcgtg actagcgccg tagtagaaaa atttactggt actaacaaag atgcagacac
     1501 gacttttatt tcacttatcc tttatcccat aacctaaact tcaatcatta cttgtgagct
     1561 gatcaatgtt cttccaagtc tgaaagaaca ttgatcagct tggcaaacaa ataatgtttt
     1621 caaatacttg atcgatgaaa aaaagctatt ggtaataaca gtaattgcat tttccatttt
     1681 cgtgtcagat acttcgctta cagctggtaa acgatcaatc ccattgtcaa attttggatt
     1741 tttttctgat gatgtcttgc tttagttcat aaaataatcc agcaataaat tcctcgcaag
     1801 gatagccaac ttcattttcc taatcggaat ttttaaactt atcggtatag atacagatgc
     1861 aatgagaaca aacatttatt tcgagtcacc atctaaaata ttttcctcca tgtacccgtt
     1921 cattattatt tagttatcac caaagatatc tctggtataa actttttctt caacatcctt
     1981 taaaatatct tcaaaccgat tagatacgat cacatcagaa atatttttaa attcattaat
     2041 atctttcaat acttgtgaat gataaaattc atcttcatcc aatgtaggct cgtaaacaac
     2101 cacttcaatt cctttggctt taatccgttt cataattcct tggatcgacg aatatcgaaa
     2161 attatctgaa ccggccttca tcgttaaacg atagattcca acaacatttg gttttttcgc
     2221 caaaatctgt ccagcaataa aatctttcct tgttgtattt gcatcaacaa ttgcactgat
     2281 gatattgctt ggtacttttt catagttggc tcttaattgt tttgtgtctt tgggtaaaca
     2341 gtatccacca tagccaaaag aaggattatt ataatgcgaa ccgatccgtg gatccaaacc
     2401 gacaccttca ataatttgct tactatcaag cccacgaaat tctgcatacg tatctaattc
     2461 attgaaatac gccactcgca atgccaaata tgtattcgag aataatttaa tagcttctgc
     2521 ttcagttgag tgagtcaaaa gaactgatgc atctttgtta aatgcgtttc tttgaaacat
     2581 agttgcaatc tcgcgaccac gagcagaaca ttctccaact ataattcttg atggatataa
     2641 gttgtcgtac aaagcttttc cttcacgcaa aaattcaggt gaaaaaatga tgttttctgt
     2701 accaaatttc gctttaattt catttgcata gccaactgga actgtcgact taataatgaa
     2761 tgttgaatct ggacgaattg ctaaagcctt ctcaattaca tcttctactg ttgatgtatt
     2821 aaaatagttt ttcttttcgt cataatctgt tggagttgca ataatcaagt aatcaccgaa
     2881 gtttactgca tcagtaaaat ttgacgtaaa ttcgacattt aagtcatccc gttttaaaaa
     2941 ttcatgaact tctttatctt ctattggtga gatgccattt tgtaacatcg tgattttttc
     3001 ttccacgata tcataagctt tgacctgctc tttttgtgct aataatagtg aatttgctag
     3061 accaacatag cctaatccga aaactgaaat tttcatattc ttccatacgc tccaatcaaa
     3121 tttcatttta tagtatttta gctgttatcc ttttgaacat attccttatt caatcaatat
     3181 tatttaatag aaaaaatttt tttgcttcaa aatcatcgag gttcgatatt taataaattc
     3241 atttttattt ttctcataat gctcctctat agttgaaatc actttacatg aactacccgc
     3301 tacaacacta ttatttggga tatctttatt tactaaagtg ttcgctctaa taattacatt
     3361 atcgccaatt gaaactcctg ggaaaacaat agagtaaata ccaataaaaa tattatttcc
     3421 caatttgatc ctacctattt ttgaagttga ctcgctcaca gaactcatct tccttaagac
     3481 ccagactcca ccatcgtgtg ttataaattt aacaccaaat gaacatctga aattatcccc
     3541 tatttcaatt aaatatggct cgctcccaaa ttcaggaacc tctactaaac tatgtttttt
     3601 tccgaatatc aaaccttcat taatacattc tcgcagattt tttccaaata gttttctctt
     3661 tattcttcga aagattctat tcttacttcg tataccctca ccttttagcc atttttattc
     3721 tgaaaaattt ttttagatac gttataatta attgtttata cgtaaaaacg aaaatagcac
     3781 taaaaataat cgttaagaaa agattaaaat agaagcctct atgaatgcat acaaaagcga
     3841 ttgcggaata gagaactaaa agtagcgata ttcgcttcca atcaaatgca taataagcaa
     3901 ttttccttcc aaaataatat ctaagtccaa ataatactaa agtaccgagc aaagtagcaa
     3961 atgagacacc aattactcca aaagaatagc ctaataaata ggtgccaaca acactcaata
     4021 gacctgacac tactgaaatc attaataact gatttgtttt attgtatact gcaaatatat
     4081 ttccgatatt tgttgtcatc ccactgaaac atgccataat aactaatatt ggaactagaa
     4141 ctaatgattt tttataatca gggttggtta taaaagagta tgcacagcaa atgataggaa
     4201 tagaaagagt cgctaaccta atgtacattt taaaaatata ggtatttttc tcaacgaatc
     4261 tttcgatgaa atcatcattc tcagacgtga taaccgcatc ctctatcata gacatactaa
     4321 caatacttgt aagagttaaa attatagatg aaaatttcat cgcaaaagaa aaaataccat
     4381 tttctgttac tccgatgtac gttccaatat aaaatcttga aaaaccaact attagccatc
     4441 caaaagtgag attcaaagtg agtgggatag agtatttcag catggacttt aaaaataaaa
     4501 aatcaaaaga actcgttgcg aaatattgaa agatctgccc cctgaaaatc aaaaaaatca
     4561 aatttataac attagaaatt atgatagaga gtaagagacc aagtatcccc atacccattg
     4621 taacaacaaa aatagtaagg agtaacaggt tgatacccgc agcgattata ccactcatca
     4681 caaaaatctt tgattcacct agtgctctta caatatattg aaaaacactt aggataggag
     4741 tgaaaattaa ttgaaaaaca aacaattgat aatactctaa attcgggaaa atcattctat
     4801 atattattgg taaaaaaatg gctaatatca ctgtatttgc tataacaaaa caaatactgt
     4861 tcgtcactac ttttttctga atattttttt ctttgcctaa tcccatgcgt aaaattgctt
     4921 cccatattgc tccaaaaata actggagaaa gaaattgagc aattgtattt tgataatcaa
     4981 agcttcctaa atcggcaaca gaaatatata ctgcatagat agggattata atgacagata
     5041 tcatttttga tgataatgtt cctattgcgt aaataaaagc ttttttagct attgaatgtg
     5101 atttattact gctactatcc aaccgtatga cctccctcaa ctcacatcta tagatgactt
     5161 tctggtttaa ttagaactac ctaaattcct ctgtatcgtg tgagtcctcg tagtaaaaat
     5221 ttttttgaat atgtgaatag ctaagatctt ttcaaataaa gatatccatg atcaactata
     5281 tatttcttca atctcatcaa tcaatattgg cagtcagaat ccagttcaca catcttgata
     5341 cacaattttg ttttatagaa attcttttag ttatagaaaa tatctattcg gcgagaaagt
     5401 tcgtcacgtt tgaaagtaga atttcctaaa atacctaaca aagctaaaaa aatcgtatga
     5461 attacccatc ttcaatattc ctttcaaaat taactcaaat aatacactgt ataccattca
     5521 acgaattttt taatgccctc atccaaggtt atattcggtt taaagttgat tttttcctct
     5581 aaatcttcaa tatcggcata agttctaacg acatcacccg gctgcatgtc caaaaaaatc
     5641 ttgttcgctt taacacctaa atacttttca attgtttcaa caaactccaa caatgcaacc
     5701 ggcttattat ttccaatatt gtatatctta tatggtgccc aactttcact actcaaatca
     5761 tcgatgtctg tccagtcgga gttgggttca ggtactttat tgattaatct agtgattccc
     5821 tctacgatat catcgatgta agtgaaatct ctttccatct ttccatggtt aaaaatctta
     5881 atttcttttc cagataaaat attcttggta aaagagaaat aggccatatc tggtcgccca
     5941 aatggaccat agacagtgaa aaatctcagc ccagttgttg gtattttata aaggtggcta
     6001 taagtatgag ccattaattc attcgatttt ttggtagccg cataaaggct aactggatga
     6061 tccaccgaat cttctgttga aaacggtact ttcgtgttcc caccatatac tgaactagat
     6121 gatgcatata atagatgctt tacagggaag tttcggcagg cttcaagaat attaagaaaa
     6181 ccgataatat tagaatcaat ataagcataa gggttttcaa tagaatatct aacacctgct
     6241 tgagcagcta aatgaattac tatgtcaaaa ccttcacttt caaatagaga atctaatttt
     6301 tctttctcta atagatccat tctgataaat ttaaaattat tataatcgta cagtatcgtt
     6361 aatcgatctt cttttaattg gacatcataa taatcattga gattatcaat cgctacaatt
     6421 tgattttcag tttccttgag taattttcta gacaaatgaa aaccaataaa tcctgctcca
     6481 cctgttatta atattttcat ttttattcat ccaccttttt ctgaaaggaa ccaatagggt
     6541 taaacccaaa attgctttct tctttatact cgtattacat ttattattta ggcatacccc
     6601 ataatttaag atataacgaa gtttatgata ctatgcgtct tctccattca ctcaaaaaat
     6661 ataacttaat tttttatgac tttaaaaagg aaatcttcta tctccaaata tttactagga
     6721 tttgaaagaa tagtttagaa atcatttagg gctaataatg acttatgatt cttaatttag
     6781 cgcttttata tacattattg cctaaggtag tcacttcctt cattgcatgt ccggacttcc
     6841 acaacacgta aggaaatgcg agctgatctc gcccactttc tgagttcaaa tactctctcc
     6901 accacaaatc tagaagattc tcagcgattg aattatttaa atcaactaaa ataaccgtgg
     6961 cttcagccaa tccaaaatta tcaggaaatc cttcttctct gtacttacta atttgctcta
     7021 taatactttc cttattacct ttgccaactg caattaatgc atctgcctca gaatataaag
     7081 attttctttc actatgttta tgcatactta acccaagttt ggaagaactg ttcagtaatc
     7141 cggtaatatc agaagttatt gttacattac catcaacata caaagtaaaa tcgtagtcag
     7201 taaatagctc attaggatga aatttaatat atctattgat caaaatatta ttttttaatt
     7261 ccgatatata tttaggtatc tctctaatat tccaatgctc acttgtaatc acattatctg
     7321 tatacaaata gtaatctacc aatggatgag aaaaatccgg ttctttaata gaatcataat
     7381 taccagtgat acaactatac acagcaatcc ttttaggtgt tccttgatta gttaactctt
     7441 tttttcttag ttcatcactg cgattctctc tgtttgaata gtctttaata ggccccttac
     7501 cggtcatcga tttaagggta tattttgctg gcaacagatt atctcgtttt ttcatttggt
     7561 taagttgatt aattttattt cgtattttca tgcctaaaat agaattattt ttttgattta
     7621 attcatgtat ttcaagattt cttttttcca attcattgat agctaaacta tataattctt
     7681 gtcttcccat aaatccctca tctctatttt taagtgctat ttattcttac tcaaaaaaat
     7741 agtaaacagc cttaaaaata atattttctc taaataatgg taggattcca tttacaaaag
     7801 cagttcctct tatattgatg aatatcaaac acacaatgat aaaaaagaaa tacgcaagca
     7861 agtagatata attctttact tttgttctct tataattact cattaataca ctggcataaa
     7921 taatataatt taatacaaat atattttgaa taattctaat aaaattatta cttataagta
     7981 agaaaactat actagtagaa gctaacatat tgatagaaaa tatatatgag cttaagctga
     8041 taactttttt ttcgtaaata ttcatttttg ataatttatg aactaaaaaa agatttgctg
     8101 aaaaaaagag agcaaacatc actacgtgag aaatcgatgt actgtttcta tcaaaatatg
     8161 catcatactt cgtatttata aatattgaac tgatataact ttgtccaata gagaatagga
     8221 gcaagctaag gattgtaaca tatccagtaa tctttttatt aatttttaca ataaaaaaag
     8281 ggagataaaa atatgcaaga ttatggaaca gtccagccac taagataaaa aaaatatact
     8341 taaatatagc tttgctgcct cgcaatttag ttagatgata aaaagctagt aatatgaata
     8401 tcaatgcgct aaacatacgt acttgtattg cttgtgaagt aaaaccaaaa ataaaaaaca
     8461 agaacaaaac taaaaaaaca ttagttgtga ataatctgat gacatacaat aaagctgaga
     8521 gtaagacagt agaaataatc aataaaaaaa tctgatactg actggaaata aaggtacata
     8581 cgtacataac agctctgaat cccggttcaa tactatctga actaaagtcc ataccaaatt
     8641 gtgctatccc attatacaag acagaatatg cataataatc agcgttttgt gtgttaaatg
     8701 ccatgatgat ccacattatc aaaagtagca ccccagacaa aatcttgttc gatgtactcc
     8761 attttttttg tgaaacactc ttttcttgat taatctccaa tgacgcctct gtatccatct
     8821 cgaacccccc ttatagccgc tctaaatcta ctgagcttat cagtctcaaa agctattgca
     8881 ataaaaaatt tcaataatct tttattcata aggcgggaaa attccttatc acttttatac
     8941 attcttttca ccaatgttga atttcttgca ataaaatact ttcgtgttga attatgattg
     9001 tctattttaa ttttttttga tcctattttt aaattttctc catctccaac gtgatgataa
     9061 aaatagactt gatttgttac ataaatgtta tagccattat ttgcaaccct tttacaaaat
     9121 tcaacatcaa tctgatcaat aaaaaattca tccttcatat ttccaacatc gtcaattaat
     9181 tcacttttga aaaacattcc agaggaattt gcaaatgcta ttttcatgga accactagtt
     9241 gaagcaaagt gtttttttac gtatctccct ctctcatttt tgcaggtaaa agagacagat
     9301 tcatcattgc tattctcaaa gtaattggga acaataatcc caatgtcctt atctttcgct
     9361 tcaatattgg taaactcttc taat
//
LOCUS       DAYYHS010000101         8940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000101 DAYYHS010000000
VERSION     DAYYHS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.103"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHA6A_002550"
     CDS             complement(13..1152)
                     /locus_tag="ACHA6A_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8970683.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHA6A_002551"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHA6A_002551"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8970684.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHA6A_002552"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHA6A_002552"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8970685.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHA6A_002553"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHA6A_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8970686.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHA6A_002554"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHA6A_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8970687.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHA6A_002555"
     CDS             complement(6166..7257)
                     /locus_tag="ACHA6A_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8970688.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHA6A_002556"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHA6A_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8970689.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHA6A_002557"
     tRNA            8800..8871
                     /locus_tag="ACHA6A_002557"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DAYYHS010000102         8798 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000102 DAYYHS010000000
VERSION     DAYYHS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.104"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            624..1487
                     /locus_tag="ACHA6A_002558"
     CDS             624..1487
                     /locus_tag="ACHA6A_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8970690.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1616..3238)
                     /locus_tag="ACHA6A_002559"
     CDS             complement(1616..3238)
                     /locus_tag="ACHA6A_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8970691.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3487..4008
                     /locus_tag="ACHA6A_002560"
     CDS             3487..4008
                     /locus_tag="ACHA6A_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8970692.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="ACHA6A_002561"
     CDS             complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="ACHA6A_002561"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8970693.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6089..6907
                     /locus_tag="ACHA6A_002562"
     CDS             6089..6907
                     /locus_tag="ACHA6A_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8970694.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7010..7207)
                     /locus_tag="ACHA6A_002563"
     CDS             complement(7010..7207)
                     /locus_tag="ACHA6A_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970695.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7376..7954
                     /locus_tag="ACHA6A_002564"
     CDS             7376..7954
                     /locus_tag="ACHA6A_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8970696.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            8049..8186
                     /locus_tag="ACHA6A_002565"
     CDS             8049..8186
                     /locus_tag="ACHA6A_002565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970697.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2681 a         1567 c         1717 g         2833 t
ORIGIN      
        1 aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca
       61 ctaagcccaa ttagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
      121 gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc caactcctcg
      181 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga
      241 ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct
      301 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa
      361 tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc
      421 tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
      481 atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat
      541 aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat
      601 gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc
      661 cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg
      721 atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc
      781 tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt
      841 tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa
      901 gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc
      961 ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg
     1021 aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa
     1081 gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag
     1141 gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta
     1201 ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt
     1261 cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg
     1321 tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag
     1381 agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca
     1441 caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca
     1501 ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg
     1561 agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg
     1621 cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg
     1681 tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt
     1741 ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt
     1801 cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta
     1861 cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata
     1921 aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc
     1981 ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg
     2041 ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca
     2101 caccccaacg tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg
     2161 ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa
     2221 aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca
     2281 ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag
     2341 aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt
     2401 ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt
     2461 cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc
     2521 aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg
     2581 tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat
     2641 tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt
     2701 ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt
     2761 gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt
     2821 gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt
     2881 ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat
     2941 ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag
     3001 tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca
     3061 tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt
     3121 tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca
     3181 catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt
     3241 tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa
     3301 ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa
     3361 ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata
     3421 atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga
     3481 ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt
     3541 gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta
     3601 gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta
     3661 aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta
     3721 gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt
     3781 gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga
     3841 gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga
     3901 attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc
     3961 tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac
     4021 aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt
     4081 atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg
     4141 tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac
     4201 ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat
     4261 gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat
     4321 gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca
     4381 tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct
     4441 tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta
     4501 tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt
     4561 ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca
     4621 ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc
     4681 acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata
     4741 ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg
     4801 tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct
     4861 cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg
     4921 taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt
     4981 gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata
     5041 tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta
     5101 ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca
     5161 cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg
     5221 gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt
     5281 cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca
     5341 gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag
     5401 aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg
     5461 cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt
     5521 gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc
     5581 tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc
     5641 acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca
     5701 gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg
     5761 ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca
     5821 tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt
     5881 tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt
     5941 agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc
     6001 aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac
     6061 atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg
     6121 gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt
     6181 ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc
     6241 tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga
     6301 agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt
     6361 tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga
     6421 aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa
     6481 cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc
     6541 gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc
     6601 agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt
     6661 tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact
     6721 agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat
     6781 ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca
     6841 ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt
     6901 aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac
     6961 cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt
     7021 tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga
     7081 ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga
     7141 cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt
     7201 gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt
     7261 gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca
     7321 attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc
     7381 taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt
     7441 tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg
     7501 attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca
     7561 tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt
     7621 ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct
     7681 ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt
     7741 ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa
     7801 taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta
     7861 tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat
     7921 cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag
     7981 aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag
     8041 ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc
     8101 ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga
     8161 cattattcac agcaataaaa aaataaacat ctttatgtaa tctgatgttc tatctattta
     8221 agaaaagcat gtttacagca gtatgattct gttgtaaacg tgtttttatt tgtggaaaat
     8281 ttaattttta gaaaagtgta tcgaaaaata gatagttagt ttttccacag tggataactt
     8341 cgtaagagat aaaaataaaa aattagtcaa agaagggcgt atagatttct tttgatagga
     8401 gatatacaac agaaatgaat aaatattaac gtgttttcaa acagtataaa acggtgtggt
     8461 ttttggaata aaaaaataac atatctcatt ttacactttt gttattttat acttaaaaaa
     8521 taacgacaca gaaaataccg tgtctcagat ttctctacta cttctaaaaa ctactgtttt
     8581 tttcctttga aatgccttca aaaagtagga tcaggcagaa tatactggca gaatcaagga
     8641 gaagggagcc taaataagga gaaaaaccat agaaaatata aagtaattac ttataaaaca
     8701 agctttttat agaaaaataa tatacctgag tagggtataa attaagacgg aattcctttc
     8761 tagattgaga tattgactag gaagaatgtt aagatttt
//
LOCUS       DAYYHS010000103         8782 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000103 DAYYHS010000000
VERSION     DAYYHS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.105"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHA6A_002566"
     CDS             <1..1087
                     /locus_tag="ACHA6A_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8970698.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHA6A_002567"
     CDS             complement(1305..2447)
                     /locus_tag="ACHA6A_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8970699.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHA6A_002568"
     CDS             complement(2452..2637)
                     /locus_tag="ACHA6A_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8970700.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACHA6A_002569"
     CDS             complement(2991..3236)
                     /locus_tag="ACHA6A_002569"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970701.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACHA6A_002570"
     CDS             complement(3230..3631)
                     /locus_tag="ACHA6A_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8970702.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACHA6A_002571"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACHA6A_002571"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8970703.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="ACHA6A_002572"
     CDS             complement(5649..7415)
                     /locus_tag="ACHA6A_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8970704.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="ACHA6A_002573"
     CDS             complement(7428..8222)
                     /locus_tag="ACHA6A_002573"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8970705.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..>8782)
                     /locus_tag="ACHA6A_002574"
     CDS             complement(8232..>8782)
                     /locus_tag="ACHA6A_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8970706.1"
                     /translation="LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLI
                     LFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYS
                     FARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILF
                     FLYPLSKDKVLENCELLKQKHK"
BASE COUNT         2850 a         1682 c         1169 g         3081 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aa
//
LOCUS       DAYYHS010000104         8737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000104 DAYYHS010000000
VERSION     DAYYHS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.106"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..216)
                     /locus_tag="ACHA6A_002575"
     CDS             complement(<1..216)
                     /locus_tag="ACHA6A_002575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970707.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNI"
     gene            complement(209..838)
                     /locus_tag="ACHA6A_002576"
     CDS             complement(209..838)
                     /locus_tag="ACHA6A_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8970708.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(831..1949)
                     /locus_tag="ACHA6A_002577"
     CDS             complement(831..1949)
                     /locus_tag="ACHA6A_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8970709.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1951..2583)
                     /locus_tag="ACHA6A_002578"
     CDS             complement(1951..2583)
                     /locus_tag="ACHA6A_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8970710.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2594..4411)
                     /locus_tag="ACHA6A_002579"
     CDS             complement(2594..4411)
                     /locus_tag="ACHA6A_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8970711.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4424..5188)
                     /locus_tag="ACHA6A_002580"
     CDS             complement(4424..5188)
                     /locus_tag="ACHA6A_002580"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8970712.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5216..5914)
                     /locus_tag="ACHA6A_002581"
     CDS             complement(5216..5914)
                     /locus_tag="ACHA6A_002581"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8970713.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5926..6705)
                     /locus_tag="ACHA6A_002582"
     CDS             complement(5926..6705)
                     /locus_tag="ACHA6A_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8970714.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6721..7665)
                     /locus_tag="ACHA6A_002583"
     CDS             complement(6721..7665)
                     /locus_tag="ACHA6A_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8970715.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7711..8490)
                     /locus_tag="ACHA6A_002584"
     CDS             complement(7711..8490)
                     /locus_tag="ACHA6A_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970716.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2367 a         1799 c         1471 g         3100 t
ORIGIN      
        1 tatatttttt ttatatccga tcgtttttat cacatgacga tttttaatca tatgcctttc
       61 tttactggat agaaattgtt ttcttatatg tgagtaatca gatacaacat taaaatattc
      121 gatactgttt tccgtcagaa tttcctctaa tatattcgtt cgactgatat aatcaaaagg
      181 atgaataagc agccactttt ttttattctc actcatctat cttcgcaccc tcaaatttct
      241 cagtagtccc acttgctact gatgttattc catcacggac aataactttc tttagcgtta
      301 aaaaaaatat ttttatgtct ttcatcattg aaatgttctc cacatattca atatcttttt
      361 caaatttttc tttccaacta attgcattac gaccactgac ttgagctaat ccggttaaac
      421 ctgggcgcac aaaatgtctt tttcgttgtt cattactgta tagtgaaaga tactcaacta
      481 acagaggacg cggtccaata atactcatat ctccttttaa aatattccat aattctggta
      541 gttcatcaag actagtcgaa cgcaatattt taccaaattg agtcaatctg atatcgtctg
      601 ataataaatt accttcttca tctctttgat ctgtcattgt tcgaaattta tacatcagaa
      661 aaatcttttc atgttttcca ggtcgttgtt gtttaaagat cactggcgat ccaagcttta
      721 ttcgaactag gattgcaaca aacgtcatca gtggccataa cattattaaa cctaaaattg
      781 aaaaaagaat atctatcaaa cgtttaaaga acttttcgta gatacctcta ttagacattt
      841 tgcatcactc gcttaattgt attagttact cgatacaaat catcatcggt cattttagaa
      901 tctgaaggta aacagatacc tttttcaaac aatgtctgag caacatctgt accaatatac
      961 tcatacttat cgaaaactgg ctgtaaatgc attggtttcc atatcgggcg ggcttctata
     1021 ttatttttat ctaactcatc aataagatga ttcgtattta ttccctttac tgttatgaca
     1081 cttaaccaat aatttggttc attccaatta ttttctggca tgaattgtat atttgacaat
     1141 ttagacaatt cttttttata aaaactatga atatatcgtt ttttctcaat tctttcattt
     1201 aaaactttca attgtcctcg accgatacct gcaacgatat tgctcattcg ataattatag
     1261 ccaatttcat tgtgttgata atggcgtgcc ttctcacgag attgcgtagc ccaaaacctc
     1321 actttttcca ccttttcttt gtcattagac acaagcattc cgccacctga agtcgtgatt
     1381 atcttatttc cattaaaaga ataaattcca tagtcgccga atgtaccagt atattttcct
     1441 ttatatgttg ttcctaatga ttctgcagca tcttcaatta aaacaacatt atgttctttg
     1501 catattttag taatttcatc tagttcagct gataaaccgt acaaatgcac tactaataca
     1561 gcttttgcgt taggatattt ttctagcgct gttcgcaaat gttcagggtt catattccaa
     1621 gtttcgtaat cactatcaac aaatactgga atagctcctt gatagatgat tggattggca
     1681 gtagctgaga acgtcaaaga ctgacaaatc actatatcat ctttaccgac cccagccgct
     1741 tttaaagcta gatgtatagc tgctgttcca gaagatagtg cagctgcatg actagcaccg
     1801 acatattctg ctaattcctc ttcaaaacca ttgacattca tacccaaagg agccacccaa
     1861 tttgtatcaa atgcttcttt tacatactct tgttcatatc cttcatcact catatgagga
     1921 gatgctaata atattcgttt attttccaac ttaatcccat ctttctatga ctttggtagg
     1981 tacgccaaca tttttagtct tattattaat attttttatt actacacttc ccgcacctat
     2041 aatgttcata tttccaattc ttagattttg tattatttta gtacctactc caatttcatt
     2101 tttatctcct aatagaacat gtcctgaaat atttacactt ggaaaaattg aattataatt
     2161 accgatttga gtatcatgtc cgatagtaga gccgatattg atcatattga aatttcctaa
     2221 aactacgtca gcagtgtaag tcgtataggg catcaaaata ttgccaattc ctaactgaac
     2281 atttatgccc actagtgcac ttgatgcaac taaattaggg aaaatcaatt tattattttt
     2341 atttatcttt tgatatattt tctctttgat atctggcgaa gcgattccca gaaaaatagc
     2401 agtttcttct tctaattcta ataacgagtc tattgttccc aatacagttt ttcccactaa
     2461 ttcttgattt tgcattttta acgaatcatc gacaaaacca atcaattcat atctagagaa
     2521 actttctagt agaaaagcaa tctctttgcc aaatccacca gctcctacaa taattgcttt
     2581 cactttactc cccctactta ttcgaagcat tcgcaaaagt gacaatgtct ttagccaatt
     2641 gttcatcgtc ttgcggcagg ctcttgacaa attccatcac tgtttgcatg gaataacctt
     2701 tgatgtttcc cacaaagatc ttctcgtaaa ctgcttcatc gtttctttcc ttatctaaca
     2761 atagttcttc atatagtttt tcccctggac ggatacctgt ttcaatgatt tcgatctcgt
     2821 cttcgctata tccgctcaat cggatcatgt tttttgctag atctacgatt ttcactggtt
     2881 cactcatatc taaaataaag atttcgccgc cttttgctaa cgccccagat tgaataacta
     2941 aacgacttgc ttcaggaatc gtcatgaaat atcgggtcat acgaaagtct gttacagtga
     3001 ctggccctcc ttgagcaatt tgttcccgga atactggaat cacactaccg cgagaaccaa
     3061 ggacattccc aaatctgaca gctgagaatt tcgtacatcc ttcttcattc aacccagtca
     3121 cgatcatttc agcgattcgt tttgttgaac ccatgacgtt tggcggattg ttggctttat
     3181 ctgtcgagac catgacgaag ttttttactt tcgctgcttt ggctgcttct gctacatttt
     3241 ttgtaccgaa aatattgttt ttgactgctt cttttgggtt atattccatc aatggtacat
     3301 gcttatgagc ggctgcatgg tagacgatat ctgggtggta ttgttccatg atctcattga
     3361 ttttcttgcg atcttggata tccgcaatga tcggcacgat ttctgtagga cacttttgat
     3421 gatgcgtgcg taattcacgg tcaatcaagt aaatcgagtt ctccccatga cctaaaagta
     3481 atagttttgc agggtcaaat tggatgattt gccggcagat ttctgaaccg attgatcctc
     3541 ctgcaccggt gacaagaatg actttgtttg tgatctggtc tttgatggat tcgatatcca
     3601 actctacctc atctcttccc agtaattcca ctacgtcgat ggtttttaat tttgatacgc
     3661 tgattttccc tgaggcaagt tcttccatag aaggcatcgt attgactttc acatgagcag
     3721 attcgactaa ctcgaagatt cttcgtaatt tttttcttga taaagaaggg atagcgatcg
     3781 ttaccatttg gatgtcatat ttttcgatca attccggtat atcttttaat gaccccagta
     3841 ctttttttcc tgaaagatag gtatttcttt tattcggatc atcatccacg aatccaatga
     3901 catggatatc ttgagccgtt ttagaaccaa gaaagctgtt gtataaaata cgtccacctt
     3961 cgccggcccc aacgatcaag gtatttttag cggaatctgt tgatagatac ctcatatttt
     4021 ttgtttcgat atagatacgc cagatcaatc ggctgccaat gatcagtaat agcgaaagta
     4081 ggtatgcaaa gatcactaaa cgtaaactgt attgtttatt tatgaaaagc aatatgatga
     4141 tacttgaggc cgcacttgca gataacgaac caaaaatcgc aatgatctct cttaaatttg
     4201 tataacgatt gattcgagta aaaactttga ataaactacc atagaataaa taaaaaccaa
     4261 tcgacaaacc gatagagatt aaaataaggt ccattggtat agctacaaac ggcttcatga
     4321 atttagctgc tgcaatattc gcagcaatca acaacaagct gtccacaaat accaatgtgg
     4381 caatttttct tcttcttgtt aatacaaaca ccctacattc cccctaaaat aaaccaaact
     4441 tcttcttttt tacctcttca tagactggat aactgatctc atccccgttg atcaggtcct
     4501 ttgcgacttg tttcatctgt gctacttttt ccttaccaaa gtccttggcg atttgctcat
     4561 aacattcttt taagtaaaag gtacgtttag agaccccatg ggcatcggaa gcagccattt
     4621 gcacgaggtt atgtttgacc atcattttgg ctgttttctg gatgtctttt ccaaatacac
     4681 cgacataact tggtgcggtg agttgagcca agcatcccat atctaggaat ggcagcaaat
     4741 gatttgggtc ttctcgaaaa tgcgcgttgc gttctggatg gacgatcact ggtgtctttc
     4801 ctagttgtaa caactcaaaa aagagccatt ctgcatataa cggcacttcc agtgtcggaa
     4861 attcgattag aagatatgta tcatctaaat ctgtaaataa aatttcatct cggtggatgt
     4921 cttcaatcag tgttccggta atacgtactt cttgtccttc caataacgtc aaaggcaatt
     4981 gtcttttctc taattctgct tgtaacgaag cgactagaga aatgacttgt gatttttcat
     5041 tgctgtattt tccattattg tgatggggtg tgcataaaat atgtgtgatc ccttgttgaa
     5101 tcgctttttc agccatggcg atactggcat ctatgttttc tgctccgtca tcgattcctg
     5161 gcaggatatg gcaatgtaga tcgatcattt ctcttgtctc ctctcttttt atttcttaat
     5221 tcccgtagta atagccagca tctctgctgt gttctgctcc attgtataca acaccaagga
     5281 ctcgcgcttg taccatatct aacagttctt ttgctttagt caatgattct tttcgtgacg
     5341 tattttcccg tacgaccaat aaggtgccat ccgtttttga agacatgatc tgtgcatctg
     5401 ttacggcaac gactggtggc atgtcgaaaa tcactacatc aaatagttgt cttgcttctg
     5461 ctaaaatctg atccattctt ggcgaactta gtagttctga tggatttggt ggtttaggtc
     5521 cacttggcag aatcgacaaa ttgtcgactg gtgttcgttg gatctcatct gctacacttc
     5581 ctgatgaact aagaacagta cttaatccgc ttgcgttgtt caacttgaac gttttataga
     5641 tgacgggctt acgtaagtct gcatcgacta gcagtacccg ttgaccagat ttggcaaaaa
     5701 caacggcaat gtttgctgca gtggtggatt ttccttctcc aggacctgat gaagtcacga
     5761 caatcgtttt gatttgctga ccagctgcgg aggcaaactg gatattcgtc cggatcgtac
     5821 gatattgttc ggcgattggt gaagatgggt ttgttaatgt gatcaaactg actgcatgag
     5881 tttgagttgc tttttgtttc tgtgttcgtg ccatttttct tcctcctaga ctcttgagcg
     5941 gcttctgcgt gatggggctg ttttatcgtt tgtagctttt ggtcgtgcag gagctgatcc
     6001 ttttttacgg ataccagcgt tcaattcttt tgctgacatt tctggaactg ttcctaagat
     6061 tgggaatcct aatgtatccg tcacatattt ttcatctttc actgtccgat caagtaattc
     6121 gaataagaat gccagaccta cgcctaccat cattcctaac actaagccaa tcactagatt
     6181 caatttatta tttggcgaaa caggtgtcgt attggcaact gcacctgaaa taatcgagat
     6241 tttatcgaca ttcatcacgt ctttggcgtt ttcttggaaa acttgtgaag ttgtattagc
     6301 gatctgttga gcagtcactg cattcgtgct tattgcctgg atcgagaaca tttgtgaatt
     6361 ttgtgactga ttgacggaga tcgcatcttt gatttgtcca gcagtcattt tcacattgtc
     6421 ttctgtttct aaacgatctt tgacttcatt tagtactaaa tcactgacga tcatatcttt
     6481 atacgtattg atcatttgca ggttcgtatt gacatcattg acatttgctg tgtctgattg
     6541 tggcagtgtc acgatcaatt gtgcttgtga actgtatttt ggtgtcaaga tgaaaaatgt
     6601 tacgattcct gagatggcca agccagcaaa cattgagatg atgattgtta ccaagtgttt
     6661 gcgtaatgtt tcaaaaattt ctttcaagct gattgtctct tccatttttt ctccactttc
     6721 ctatttattc tctagattca tttgatcttt taattctttt ttcacacgat caagttcttg
     6781 atcgctgact ttttgatagg agatattatc ttgcatgaag ccttctcctt gcagctgatc
     6841 ttgcttgatc gtaccaaatg cctcccgata atctaaagcg atttttttca tttcatcaaa
     6901 gctcatgtct gttttcatat tcgactctag agcattcaaa acggattgat agttgctgac
     6961 accgtttaaa ctcaatactt tttttgctac accttctacg atctttcttt gacgctcttg
     7021 tctaccatag tcaccatttg gatcttcata acgcatacga gaataagata atgcctgatc
     7081 cccgttcaat gtaattttac cgattggaaa gtcatagcca tcttgtgaga aagtgaggtc
     7141 attattgact tcgataccac caactgcatc aacgagttcc tttagccctt tcatattgat
     7201 agagacatag tgatcgattg ggatgtctac atatttttgt agggtatcca tcgccatggc
     7261 agctccgccg aatgcgtaag cgtggttgat cttgtctttt gtgtcacgac ctacgatgtc
     7321 gacatacgta tctcgaggaa tgctgacgat cgttgtctgt ttgtcttcag gactgactgt
     7381 ggcaaccatc atcgtatctg atcttccttg atcgacccga cctaaatctc cagtatcaat
     7441 gcccatcaat agaacagaga aagattcttg tttgtccaga tcaactggtt tcgttcgttc
     7501 tccttgggtt cgttccactg attcgtaagt tttctcaata gaatgtgaga gatccagata
     7561 taattttgct cctactccta caattgctaa tactaatacg ataagaactg ctaaaataat
     7621 cagaaatatt tttttccctc ttgattttct tcttcgtctt gccatagttt cctccgtttg
     7681 ctaaccttgc ttgaatcaac gttttaactg tcaataactt atattgctat agcccatttg
     7741 ctgagcaaca gtttgtgctt ttgttgcgta tgctgcgctt attttttcat tccggatctg
     7801 ctctatttga ctgagcgccg tcaaatattg atcgtaagta gcggtggcag tcacttgacc
     7861 atccttcaac atcgtatcga ataactgatc tagttgcgtg acttgcgcga tctgagtatc
     7921 agcgaatcct agatattgca taacaaccgt tttccaagga ctatctttaa aaaagctcat
     7981 attttcacgt acacgagcga catctgcact tgtcaccttt tctttgatga cgacatcgtc
     8041 tacagcttgc tgccagtttg atacattttt agtaaataaa tcgttgacag ctgtctggat
     8101 cttcagtttt gattctgcac tatctaactg tgtcacgatt gctttcttct catcttggat
     8161 tgcaacagct tctttaggca attctgattc tttgatgtcg taatcatctg ctgaaagttt
     8221 gacagaggat agcttatttg acaaacttgt gattgtttct tctgttattt cagggtttag
     8281 aaaatttttt tcttttgtac tatacagctt gtctacttct tttgataaag aagtcaaggt
     8341 gtctttttga ttggtgatgg cagattctgc ataattgatc cgttgctgat gttgccgctg
     8401 attattgata tggtaaccaa acaaacagat cgcaatagca cctgcaccta ccgccacagc
     8461 agtcaatgac ttgacgaatg gcttttccat agattcactc ctaagtattg atgatagata
     8521 aattaagata taattcacat acataaaaat gacagctttt tattttaaaa aacataatta
     8581 aattaatata ttcacagaaa tatttgtaaa caagttttcc ctatataatc ataccacaaa
     8641 ttgttttctt gactagggca atttacgatt taaaagtaaa taaaacatta ataagataac
     8701 aattaatcga tttcattttt atttccttaa ttttcat
//
LOCUS       DAYYHS010000105         8675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000105 DAYYHS010000000
VERSION     DAYYHS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.107"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHA6A_002585"
     CDS             <1..1101
                     /locus_tag="ACHA6A_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8970717.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACHA6A_002586"
     CDS             complement(1280..1615)
                     /locus_tag="ACHA6A_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8970718.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACHA6A_002587"
     CDS             complement(1599..1862)
                     /locus_tag="ACHA6A_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8970719.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACHA6A_002588"
     CDS             complement(2322..3125)
                     /locus_tag="ACHA6A_002588"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8970720.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACHA6A_002589"
     CDS             complement(3103..4227)
                     /locus_tag="ACHA6A_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970721.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACHA6A_002590"
     CDS             complement(4243..5058)
                     /locus_tag="ACHA6A_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970722.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACHA6A_002591"
     CDS             complement(5048..5920)
                     /locus_tag="ACHA6A_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8970723.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACHA6A_002592"
     CDS             complement(6005..7294)
                     /locus_tag="ACHA6A_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8970724.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7950)
                     /locus_tag="ACHA6A_002593"
     CDS             complement(7438..7950)
                     /locus_tag="ACHA6A_002593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970725.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACHA6A_002594"
     CDS             complement(8017..8160)
                     /locus_tag="ACHA6A_002594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8970726.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAYYHS010000106         8645 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000106 DAYYHS010000000
VERSION     DAYYHS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.108"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..2624)
                     /locus_tag="ACHA6A_002595"
     CDS             complement(243..2624)
                     /locus_tag="ACHA6A_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8970727.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2614..3261)
                     /gene="recU"
                     /locus_tag="ACHA6A_002596"
     CDS             complement(2614..3261)
                     /gene="recU"
                     /locus_tag="ACHA6A_002596"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8970728.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3325..3864
                     /locus_tag="ACHA6A_002597"
     CDS             3325..3864
                     /locus_tag="ACHA6A_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8970729.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3954..4373
                     /gene="gpsB"
                     /locus_tag="ACHA6A_002598"
     CDS             3954..4373
                     /gene="gpsB"
                     /locus_tag="ACHA6A_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8970730.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4395..4762
                     /gene="rnpB"
                     /locus_tag="ACHA6A_002599"
     ncRNA           4395..4762
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA6A_002599"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4971..6137
                     /locus_tag="ACHA6A_002600"
     CDS             4971..6137
                     /locus_tag="ACHA6A_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8970731.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6183..7679
                     /locus_tag="ACHA6A_002601"
     CDS             6183..7679
                     /locus_tag="ACHA6A_002601"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8970732.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7760..8443
                     /locus_tag="ACHA6A_002602"
     CDS             7760..8443
                     /locus_tag="ACHA6A_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8970733.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8452..>8645
                     /locus_tag="ACHA6A_002603"
     CDS             8452..>8645
                     /locus_tag="ACHA6A_002603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970734.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2717 a         1569 c         1836 g         2523 t
ORIGIN      
        1 ataaaagact agtgtgtatt tagccgattt aattataaac aagtaaatgg ataaagtata
       61 tgtaatgata aaagaaaaat ggattttcta atttttaagc taaatgttag atcacacatg
      121 tatatgctgg atagtcttat aaaaaatcat gtaatacagc ttggtacatg atttttattt
      181 gtagctgttt tttacataga cacaaaaaag aaaccacgct tgtagacgtg gtttcttttt
      241 tactatctgt ttccagaagc tgaggtgctg ctggatggtg tatggtcatt tgcagcatcc
      301 gctccgctag ttgctggctc actggaagaa gcaggaggag cagatgaaga cggtgtcgag
      361 ctttcttcgg atgaagaaga aggaaccgtt gttgaagacg ctggctcgct tgtttctgct
      421 gtcgtgcttt cttttgagga ctcagcagtt gattcgcttt gactagtaga gctagagctt
      481 tgagtcgttg tctctgttgt tgagcttcca ggagtctgga catagctgga agatggaata
      541 gttgttgatg gtgtaattgc attcgatcta gctgtatatt gatctttgta gtacagttca
      601 ccgcccacac gaattaatcc gcttggcatc tcccagtctg tatttgtcac gttagctgaa
      661 acatactgca taagctcgcg ataaacatca gaggctacgt gtgacgattc tgaagtgaca
      721 ggcgtcattt ttttattgta acctgtccac acggaaatcg aataatttgg cgtatagccc
      781 gcaaatagga tatctggata caccccagaa gaaataccaa gttttgcata ctcgtcatct
      841 gtgtaattgg atgttccggt cttaccggct tgatataacc cagcgatttg cgcattcgta
      901 ccagttcctt cagtgatcgt atcttttaaa atgtctgtga tcatataagc tgtttctgga
      961 gacatggctg tttttccatc tggctcataa gtttcttctg ttccgtcttg gaagacgatt
     1021 ttattaacat attgaggttt gtaataagtc cctccattgg cgaaagccgc ataggctgct
     1081 gccattttta aggaagaagc tccgtatttt gtcccatctt gttcttccgt attactagag
     1141 atcgcattcg attgatggat cgtactgtat tcgatcccaa gattttttaa gaaagaagcc
     1201 actttatctg aaccgacttc attgaataat ttgactgctg gaacattacg ggataagtaa
     1261 agtgcttggc gtaaagtaat ggtgcccata tattgattat cccagtttcc tacaggagtc
     1321 gaagtccctt catagttata cggtgcgtca gtaatagttt ttccagtaga atatttcaaa
     1381 tattcaaaag ctggtccgta atctgtgaca ggtttcatcg tagaaccaaa atcacgggaa
     1441 gtattcactg ctaagttgtt gcctaaagtt acgtcttctg cgatgtgacg tccgccaatc
     1501 tgggctttta ctttcccggt attggtgtcg ataagggtgg aggcaacttg catctcgtcg
     1561 tctgggtaag agacatactg atccgtgttg acgatatcat atagtttctt ttgggcgtcc
     1621 agatccaaat tcgtataaat ctctaaaccg tctgtgtaga cattcttatc tgttttttct
     1681 tgtacttcat tgatgacttc tttgacataa ttatcgacga tcttagtatt gtcatcggat
     1741 tgagttagtt cttgcagacc atccgttacc ggtacattaa cagcttgatc atattctgtt
     1801 tgagagattt tttcgttttg aagcatcgta tacaaaacag tatctcgtct tttcttcgcc
     1861 tgatctggat aaacataagg atcataggcg gaaggggctt gaggcatccc agcaagcaat
     1921 gctgtttgcg gtaaagaaag ttctgataat ttcttaccaa agtacatctc agaagctgtc
     1981 tccatcccgt acaagccgtt tgacatatac actttattta cgtagtaggt caagatctct
     2041 tgtttcgatt ttttttgttc caatcttaca gccatccacg cttcttgggc cttgcgtttc
     2101 agagtctgat cctcggcact ggtggagaag aaggaaagtt tgatcaattg ctgtgtcaaa
     2161 gtacttccgc cttgcagtcc gccagaggta aagttagaga gggcagaacc gatgatccga
     2221 atagggtcaa ccccgatgtg tttataaaaa cggcgatctt ctaccgacac gatcgcatct
     2281 tctaatgttt tcggtaattc attcgcagaa attttttcac gcttttctgc gcctaaatct
     2341 tcgaatagtt cgccatcttg tgtatatagt ttggaagata cggtagcgtc taatttttta
     2401 tccgtcaatt cgggcgcatc ttttgcgtaa taccagaaaa gtcctacacc tgctaaaaag
     2461 aggatacaga aaaatgagag tattcctaaa aggatcttga taaataatcc ttttttatgt
     2521 gttccagagg actttccaga acctttgcca gaattcttct tcacactctt tttaggagta
     2581 ggctgtttct gtcgtctaga gcttctagtt tgttcatttg ccattcttgc tttctccttc
     2641 aatagataat gttgataaat agaagtcaac cgcgtccaaa taaggaatcc gcggagaaat
     2701 accagttttg atctctattc ctagttcttc gatcaaagat aaaggtagtg atttgcgtcc
     2761 tttttccttt tgggcgtccc agtaagcaat caaatgaaca ctattcaaat aaaaacagcg
     2821 cttcaaagac gaaaaccata acaagacaaa gcagacccca ttttgttcga ggcatgcttt
     2881 catatgttcg atttgatggg catggaagtt tttaaaggga aaagatgttt tgttcttggt
     2941 ttcttttgct tcaaagtcca agtaaaagcc tcgatagacg ccattatagt ctgtagttga
     3001 ggcttgggta aagtaggctt ccttgatcac agcagcactt ctcttaggat agtctacttt
     3061 cacgatttgg ataggggtag gctttttatg gataaccgcc aacccgcggg aaagataata
     3121 tttattactt tcattgattg cttcttcaaa gcgcatcccc cgattgccga attcagtgat
     3181 ctgatttctt ttttcttgaa ttttttcgac gggagaggca ttcttataat aaggttggcc
     3241 atttggatag cgcaatacca tctttaatca cactcctagg ccacattata gcaaaaaagc
     3301 ggatagaaag aaaaggaatc tgagatggat aagtcaaaag taatctatgt gtctgggtat
     3361 cgaagtttcg aactcggtgt ttttaaagaa aatgatccta aaatccaagt tgttaaaaat
     3421 gttttaaaaa aagaactcca tcagttagca gaagaagggc tggaatgggt acttgtcagt
     3481 gggaatttgg gcgtcgaact atgggctgtg caaatagtag cggaattgaa agaagaatat
     3541 ccggaactat ctcttggtat tttgtatcct ttttctgatt ttggtaataa ctggaatgaa
     3601 gggaaccaag aaaaaaaaca attagcagaa caattagcag attatgtcga agctgttagc
     3661 catcaaccct accaatcacc tagtcagcta aagaatcaca ctcgttttat acttgagcat
     3721 acagtgggct gtctgctgat ctatgatgaa gaattccctg gaaaaacgaa gtattttttt
     3781 gaagatgcac agaattacca agcagatcat ccctacgaaa ttcggctgat cagtatggac
     3841 gatcttcaaa attttagtga ataatcgata ttgtgtttga tttatcaact tttttcctat
     3901 acaatgaaaa aggtagaaat gttattacgt aaatgaaaac tagaggtgta aatatggcga
     3961 atttggttta tagtcctaaa gacattttac aacaagaatt caaaacaaaa atgcgcggct
     4021 atgatcccgt tgaagttgat gaattcttag acaatatcat caaagattat gaaacttaca
     4081 gcaaagaact ccttgcttta caagaagaaa acgatcgatt gagtgcaaaa gtagctcaat
     4141 tatctaaaac tcaaggagca gctcaaacac gcgtgcaaca aacagaggca ccaaagagcg
     4201 cagcagtgac caattttgat atcttaaaac gcttgtcaaa tcttgaacgt gaagtatttg
     4261 gtaaaaagct tgatcaacaa gcaagtgcag tcaaaccagc acaacctaat ccaaataact
     4321 acacaaatgc agacacaagc ttagatgata atgaaaaaac acgtcaattt taatttttga
     4381 ttaatgaatt tgcggttttc gggtaatcgc ggtctgactt gatcaggctg aggaaagtcc
     4441 atgctcgcac aggctgtgat gcctgtagtg ttcgtgctta gcgaaaccat aagctaaggt
     4501 actctttgga gtaacggcag gaaaacaagc taaggcttcg gctatgctta agtatccttg
     4561 aaagtgccac agtgacgaag cggctgggga aacccatccg gtggaacgcg gtaaacccct
     4621 cgagcgagca acccaaacaa tggtaggggc gctttttgtc tggaagaaga acaggacaaa
     4681 aaggcagtat aactgcagat agatggttac cgctttgttt tttctccctg gaaaagcaaa
     4741 gtacagaaca tggcttatag aaaaccgcaa acatcaggga attaggtcgt gacaaaagta
     4801 atgcgttata agaaacaaga aaaacatttg aaagctattt ttcaatactt cagatgaatc
     4861 gttcttatta caaaacaaac ttttgtatca ccggttattc ggaatttgag taaaagtgtg
     4921 gggtaggact gatgtcccac ctcctttttt tataaataag gagattgtgt atggaaaaaa
     4981 acaggaagtt caacctcgtc gcaactgcgg caagcgggct agaagcactg gttggtaaag
     5041 aattaagaga tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt tttgaaggaa
     5101 caatggaaac gatcgctaca gctaatttat ggttacgtac agcagatcga gtaaaaatcg
     5161 tggttgggga attcgatgcg tatacgtttg atgaattgtt tgaaaacgta aaagcattgc
     5221 cttgggaaga ttttcttcca ctagatgcag cttttcctgt agctggaaaa tcgatcaagt
     5281 cgaaactata tagcacacct gattgccaag cgatcacaaa aaaagcaatc gttgaacgtc
     5341 tccgtaccta ttatcaccgt ccagcttctg taccactgac ggaaactggc gcacattttc
     5401 agttggaagt agctttgctg aaagaccatg tgatggtaac tctggatacg acaggaccaa
     5461 gtttattcaa acgtggttat cgcttagaaa aaggtggagc accattgaaa gaaaacatgg
     5521 cagcagcact tattatgttg acaaattggc gaaaagaccg tccattttat gatcctgtat
     5581 gtggttcagg tacgctttgt attgaagcag ccttaatagg ccataatatc gcaccaggat
     5641 tcaatcgtga gtttgcttgc gaatcttggg attggttcag tcaagaaatc atggaaaatg
     5701 ttcgatcagc agccgaagaa aaagcagatt atgatattga attagatatt actggttcag
     5761 atatcaatgg acggatgatt gaaattgcca aagccaatgc agaagaaatc ggattaggct
     5821 cttcgattac ctttaagcaa caagcagtca aagacttcaa aacggataaa gaatacgggg
     5881 tcatcgtggc taatccgcct tacggagaac gtctaggaga agaagaaact gttcggaaat
     5941 tatataaaga aatgggcgat gtattccgtc cattaaaaac atggagtaaa tatatcttga
     6001 ctagcgactt agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa agaaaattgt
     6061 acaatggtgc attgcgtacg gatctattcc agtattgggg aactcgtccg cctagaaaaa
     6121 aagagaaaga cgaataatct tactaattga ttcattcaat tggatgtttg gaggaatagg
     6181 gaatgaaaga aaaagagttt ttacaagaag taaaagaaat caatcttcta caacaagctt
     6241 taggaatcct cgattgggat acacaaacag gtatgcctga aaaagctagt gcataccgaa
     6301 gtgaagtaga cagctatctt tacagcattt atttttcaaa aaaaatcggt ccaaaaatca
     6361 aagaagccat tcgctatttt tctgaacatc cggaagaatt gtcggaagtt ggaactgccg
     6421 tttttgccaa agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa gatcttatga
     6481 gaaaatatac agctgccgtt tcttcagcac acggacaatg gcaaaaagct agagaaacac
     6541 aacaattttc tgattttcaa cacgcattga atcaaaatat tgaattaaca aaacagttga
     6601 taccttactg gagaaaagat gaaaagacga attatgatgt tttgctgaat caatacgaac
     6661 ccgggatgac tgtcgaaatc cttgataagg tatttgatca gcttaaagaa ggaattcttt
     6721 cgatccgccg tacgctccaa gaaaaaggga ctgaacctga aacagacttt ttaaatcgga
     6781 gaatgacgaa aaaacaacag aagagatttg tagtgaaagt aatcgaacaa cttggttatg
     6841 attttagtcg tggtcgttta gatgacactg tccacccatt tatgactgga attaatccga
     6901 atgatgcacg aatcacgaca cgatggaacg aaacagattt caaaatggct gtttttggag
     6961 tgatccacga ggctggtcat ggtatgtatg aacaagacat cgatgataaa tatgcgtata
     7021 caccggttta tgaaggtaca tcaatgggta tccatgaatc acaatcgttg tttaacgaga
     7081 tcattattgg aagtagtcgc agcttctgga aaaagcaata tcctttcttt caagaatgtg
     7141 cagaaggaac attcgatgat attccttttg acaaatttta tcgttcattg aaccatacaa
     7201 aatcaagttt ggttcgtatc gaggcagaca gtttgactta ccctctccat atcatcattc
     7261 gttatgaaat cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa ctacctgaaa
     7321 tatggaatca aaaatatgaa gagtatctgg gaatacgacc aacaaatgat gtggaaggca
     7381 tcctacaaga cgttcattgg tcaggtgcaa gctttggtta ttttccctct tatgcattag
     7441 ggtacatgta tgcggcacaa ttacttcatt ctttgcagca agaaatagat gtggaaaatg
     7501 ttctagcgtc agacgattat tctccaatca aaaactggat gtcagagcat atccatcaat
     7561 atggtgcatc tagaaaacca aatcaattaa tcatggatgc aacacatgaa ccgcttaatc
     7621 cgcaatacct gatcgactat atgaaagacc tgtattttaa tgtatatcag gtaaaataaa
     7681 atacaattta caaagatagc tgaaattcat ctaatctata gatgttttca gctatttact
     7741 taagaaagaa gtggaacgaa tggataaatt acaagtgatc gctgattata gtatggacca
     7801 attagcgcat gatcaaacag gacatgggag cgatcatacc aaaagagttg tcaagttagc
     7861 tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg cagcagctta
     7921 cctacatgat acgattgatg ataaagtcgt taaaaatgag aacgaggcaa agcaacagct
     7981 tcgtgtattc cttcgctctt tgccaatagt agaggaacag atctcgatga ttttttccat
     8041 tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg gccgaactat ctctggaagg
     8101 taagatcgtt caagacgctg atcgtatcga agcattagga gcaatcggca ttttgcgtac
     8161 tgcttatttc ggcggtggac atgggcaccc gatttttgat cctgaactgt atccgcaaac
     8221 attcaaggac aaagaaaatt accgaaaagg cacgacagtg atcaatcatt tttatgaaaa
     8281 actgttcttg ctgtctgata aaatgaatac tgattacgga tatgaagaag ccaaacgaag
     8341 agaaaccttc atgcgtgaat tcttagaaga attttttctg gaatggtcta ttgacgatga
     8401 aaattttact ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt tgtgacagaa
     8461 gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa gttacttctg gaacactgtt
     8521 taccaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaat
     8581 agcttctcgt gtttgatgaa tattgtaact tgtgattcgg aattggcagg agaacaccgt
     8641 ttatt
//
LOCUS       DAYYHS010000107         8587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000107 DAYYHS010000000
VERSION     DAYYHS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.109"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1512)
                     /locus_tag="ACHA6A_002604"
     CDS             complement(46..1512)
                     /locus_tag="ACHA6A_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8970735.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1899..2495
                     /locus_tag="ACHA6A_002605"
     CDS             1899..2495
                     /locus_tag="ACHA6A_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8970736.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2553..3035)
                     /locus_tag="ACHA6A_002606"
     CDS             complement(2553..3035)
                     /locus_tag="ACHA6A_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970737.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3141..3338)
                     /locus_tag="ACHA6A_002607"
     CDS             complement(3141..3338)
                     /locus_tag="ACHA6A_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970738.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3633..4391
                     /locus_tag="ACHA6A_002608"
     CDS             3633..4391
                     /locus_tag="ACHA6A_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8970739.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4451..4879
                     /locus_tag="ACHA6A_002609"
     CDS             4451..4879
                     /locus_tag="ACHA6A_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970740.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4925..4997
                     /locus_tag="ACHA6A_002610"
     tRNA            4925..4997
                     /locus_tag="ACHA6A_002610"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4958..4960,aa:Gln,seq:ctg)
     gene            complement(5147..5518)
                     /locus_tag="ACHA6A_002611"
     CDS             complement(5147..5518)
                     /locus_tag="ACHA6A_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8970741.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5733..5918
                     /locus_tag="ACHA6A_002612"
     CDS             5733..5918
                     /locus_tag="ACHA6A_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970742.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6001..6189)
                     /locus_tag="ACHA6A_002613"
     CDS             complement(6001..6189)
                     /locus_tag="ACHA6A_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970743.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6213..6476)
                     /locus_tag="ACHA6A_002614"
     CDS             complement(6213..6476)
                     /locus_tag="ACHA6A_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970744.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            complement(6464..6607)
                     /locus_tag="ACHA6A_002615"
     CDS             complement(6464..6607)
                     /locus_tag="ACHA6A_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970745.1"
                     /translation="MIQNNTLCEMAQAVLWIYPVKSVSILKLEEKLAIIKVKEGVGFS
                     WIM"
     gene            6634..6891
                     /locus_tag="ACHA6A_002616"
     CDS             6634..6891
                     /locus_tag="ACHA6A_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8970746.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6962..7153)
                     /locus_tag="ACHA6A_002617"
     CDS             complement(6962..7153)
                     /locus_tag="ACHA6A_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970747.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7294..7614)
                     /locus_tag="ACHA6A_002618"
     CDS             complement(7294..7614)
                     /locus_tag="ACHA6A_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970748.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7637..8071)
                     /locus_tag="ACHA6A_002619"
     CDS             complement(7637..8071)
                     /locus_tag="ACHA6A_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8970749.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8406..>8587
                     /locus_tag="ACHA6A_002620"
     CDS             8406..>8587
                     /locus_tag="ACHA6A_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8970750.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2719 a         1695 c         1361 g         2812 t
ORIGIN      
        1 caggaactgc tgcaaataag caattcctgt ttttatgttc attgattaat gaatagagcc
       61 cattagtttt ttgattggtt ctttcacagt aaatagcaag ataccaccga cgattgctac
      121 tgcaccgata ataccgaagt aagcagattc tgttcccggt gtgtagaagc gtgcaatttg
      181 tgcattgatt gcttgtgaag aagcatcagc taagaaccaa atagcgattg tttgtgattc
      241 aaatgctcgt ggtgctaatt ttgttgtcac agacaaacca actggtgaaa gacacaattc
      301 agcaataacc acgatgaaga aacttcccaa caaccacaat ggacttactt ttccacttgt
      361 accataaagc atacccggca acatcaatag caagtacgat aatcctgcaa ataaaagtcc
      421 aagagagaat ttcactactg tagatggttg acgttcacca agtttcgtcc acatcgttac
      481 gaatactggt gtcagaataa agatgaaaat tggattcaat gattgatacc agcttgcttg
      541 gatcgttaca ccaaatagcg tagattgtgt acgttcacta gcaaataaag ctaagataga
      601 tgaaccttgt tcttgtaatg accagaaaat gattgctgca aggaataatg ggatataagc
      661 ccatactctt ggtttttctt cagcagttac atcttttgaa gtcagcattt tgataaagta
      721 atacaacggt aagataatcc ccaatacact gattgtattg acaaaaaagt caattgtcag
      781 atgaccagtt agttgtgccc cgccgaatac tgcagcgata acaacaagtg cgatgatcaa
      841 gttacggatg aatcctttac gttcttcagt tgtcaaagga ttcggcggtg ttgtacccac
      901 tccgtctaat gtcttgcgtc cttggatgat gtattgtaat aatccaaaga acatcccaat
      961 agcagctaaa gagaacccta aatgatagtt atattcttga ccgattgtac caacgattag
     1021 tggtgctaat aatgccccta agttgacacc catgtaaaaa atcgaaaatc ctgcatctct
     1081 tcgcaagtca gtctttgaat aaagatgtcc gaccatgcca gaaacatttg gtttcaaaaa
     1141 ggcggttccg ataataatca atgccatcga aacaaacaat gtacttacac caaatggtaa
     1201 agccaagacg atgtggccaa gcataatgaa aactccaccg aagaatacag tgcgttttgc
     1261 tcctaacact cggtcagata cccatccgcc gacgatacta gacatgtaga tcaaagatcc
     1321 gtagatcgac atgatagcta atgctgttgc tcgtggtaat ccaagtcccc cgttggcaac
     1381 tgtatcgtac atataataga taagtaatgc acgcataccg taataactaa atcgttccca
     1441 catctcagtg aagaacaaag ttgacaatcc cttcggctgc ccgaagaagc ccttgtcttg
     1501 ctgtttttcc ataatctata ccccaattct ttaaattttc agaaaatatt atacatcttt
     1561 accgaaaaat ttaactaaaa aacttattta agaaatataa actaattttc tataaaagca
     1621 agtaattttt aatggcaata tcataaaaaa aagaagatag agatttaata attatatatc
     1681 agctgttttt ttcaaataag caaaaattcc taaaacacaa caatgaaagc gataactaag
     1741 aaaatcctta cataacgatt tttcttatta aaattgtttt ttgaacttcg cttatttttt
     1801 gtgagcatat ctgtttactt actttttgta aggtgctata cttcatttcg taatcaatta
     1861 aaaaaatgca aaacgcaaat caaaggagaa attttattat gacaactttt actgacactt
     1921 tgaaaaaccg ccgttcaatc tatcatctag gacgtaatgt ttcactttct aatgaagaat
     1981 taacaacttt gatcaaagaa gcaattaaag aaagcccaac tgctttcaat gctcaatcaa
     2041 cacgcgcagt tatccttttc ggagatgcac atgaaaaact ttgggaaatc actgaagaag
     2101 cacttcgccc attgactcca gcagaagctt tcccaaatac acaaaacaaa ttagctggtt
     2161 tcaaaaatgg ttatggtaca gtattattct tcaaagatac tgacgtggta aaaggtctac
     2221 aagaacaatt tgaattatat gcagacaact tcccagattg gtcagaacaa tcaaacggaa
     2281 tcgcaacagc taatacttgg gtagcattag ttgataaagg attgggcgca aacttgcaac
     2341 attacaaccc agttattgac gaagcagttg caaaagaatg gaatatccct tcaaactgga
     2401 aactacgttc gcaattagta ttcggttcac cagaaacacc ggctgaggaa aaagaataca
     2461 tgaacgatgc cgatcgcttc cgcgtatttg gataatttta tttgaaataa aaaacagagg
     2521 ttgtgacaaa catcttgtcg caacctctgt ttttattctg gatcaatcat aatatcaatc
     2581 tctgcctcat cgtttaagcg ttgggacaaa aatggaacta attcttctaa tgatgcagaa
     2641 aatttcagca tcggtcctcg ccatccgttt cggaaaaaat tcatttttac gattttagaa
     2701 ccttttggct gatataatac aattttattg atctcagaaa aaggaacacc ttttttgtca
     2761 aagctattca aaacaagtcc ttcttctgtg attcctctac tgtccaacaa aaaactaata
     2821 aaaatcattg ctgacaagat cccttttgcc aattcatctg ctgtaggatg actgatgagt
     2881 gtataccaaa tgactagtga cactaagaag ggtatcacca agtagaccca tcgatttcgc
     2941 acagtccgat aaataatttc ttttcgaacc agccaaagaa ataaaaacat cacaacaacg
     3001 aataaaaaca atagaaccgt aaagaatgtt accatgacaa ctcgcctcgc tttttcttct
     3061 ccagaaaata ggaaaagaat actgatttac cttctcaaag aaattggact tgcagcacgc
     3121 cgcacgtcca attgttttct ttattcaaat gtatcatatt ttgcaacaac catgcttgct
     3181 ttttcatcta ataattcttg gtcctctgca gcacattggg caactttgac cacggcgcag
     3241 tttgtcattt ttgatacaac ttcaccaatg accgtttttg taaaaccaat tggtttgcat
     3301 gcatattgtt ctcctaaatt aatcatttct tccgtcatca aaatcctcct aaactgctgg
     3361 aacttttctc cacattacca ctgctatttc tttggagatt taactcattg atttttattt
     3421 acagttaaaa atcaacctta caggcattat agcgtactgt tttcgttttc acaattaaaa
     3481 cagctgttgt atttagaaga atttttttca aaaaattaaa acatattcct taaaaactaa
     3541 ttatttccta catattaaaa tataatactt ccatttccaa taaaaataca tttataatag
     3601 gaaagataaa cagctgaaaa gggagatttc aaatgaagaa aaaaacgttc aaaaaactat
     3661 tgtttctagg gggcacagct gctcttttat taagcggtac aagttattac ctacatatcc
     3721 attctccaga acatacaatg aacactgcac aaaaaagttc aacacaaaaa acgaaaaaga
     3781 caaaagagaa agcagaagtt ttactagatg ttccattaga gagccagttt gattctcctt
     3841 ctttagaaaa tggctgtgaa gtcacttcgc tttctatgct tttatctttt tatggttatg
     3901 aaacaactaa aaaccagtta gctgagcaat tggattacgt ccctgttttc aatgcagatg
     3961 ggactcatgg cgatcctaat gaaggatttg ttggtgatat cagtggcgga gactgggcta
     4021 tggtcgtcta tgttccccca gttgcctccc ttgctcagca aatcgtccaa acggattatc
     4081 acacttttcc aaaaaccgat gctcaactcg atgatatcaa aaaagcacta tctaaaggaa
     4141 atcctgtctg gacttctgtg actatcaatt ttgaagtccc agatgaaact gattttatga
     4201 cttggaccac aaataatgga gaagtaagag tcactcctct tgttcatgct tgtgtagtca
     4261 ctggatatga caaagaaaat atttatgtca acgatcccta tggtgtaaaa aatcgagcag
     4321 taccgatcaa cgatttttcg gcgattttca ctgcaatggg tggacagatg ctaactttgg
     4381 agaaaagcta gtaattcaaa caaaaatccc ttatactcaa gataccaaac ctttgaaggg
     4441 agcgtagata atggaaaaat tcacacataa aaaaatggat ccaaatgaaa ttccgattat
     4501 ttttgtccgt gactgcaagg gaaatgttca aggtaaagtc tctattaatg agtggaacga
     4561 gcgtcgcaga cctgctaccc tcaatgaact ggaaatcaaa ttgtatcgtc aatctcttgt
     4621 ttattacgca gatcaagagt acgagaaagc caccgattta ttgaaatttc taattgctcg
     4681 aacagagtat actcgctttg aatatattga acgtttagca aacatctatc atattatgaa
     4741 tgaacctgtc aaagaatatc agttattgga caccgtcttg tcagtagcag aactgattgc
     4801 ccttcctgct ggattagaaa agaaactagt acgccgcctt ttacgagtaa agcaacaact
     4861 atccgatcaa gaaaaataac ttgcgttttt ttaggaaagt gctatgatta ttttgttgaa
     4921 taaatgggat gtagccaaac tggtaaggca cttgactctg aatcaagcaa ttgtaggttc
     4981 gaaccctgcc atcccaattt gatgtgcttg ctgaaaaaat gaaccccctg taaaagaaca
     5041 atctttttgc agggggatta ttgtattatt tttactactt gtatttagtt tacataatta
     5101 aattatgaat aataaaatcg ggcgtattct taatacaaaa actgatttac ccaataattg
     5161 ctaatgcttt ctccggaact ttttctctag gaacttcctc atacgtttct atgtgtgctc
     5221 ctttattgct aattttcaaa taatgatttt cttttaacac agaaagacca ttaaattcaa
     5281 ccgttcgttc tttcccattg gcatcagcag ctttctgcac ataccttgct gtaccatatt
     5341 catttacttc ctctggtttt tgtgtcttta catagacctc tcctttttca actaatggat
     5401 ttagctgatc aagtatacca gggatttctc cataacttcc ttcagtataa tatttcagtc
     5461 caaatagcaa aataccacta atcgccgcca gtccaatcaa atatttaatc actttcatct
     5521 ttctacctcc ttgttatata ttcagtatat gggtcagtta aagaaattcc atccgtccca
     5581 agttggagat ttttctccta ctttagaagc gagaatcgag tgttttttta gtaaaaatga
     5641 tcgttatttg atattattga cgtgtggata aatcagtttt tctcgttttg tccatgtaaa
     5701 aaatcatgtt gatagcaaag gatgtgttca aaatgaatca agaagaatta tttcaaaaag
     5761 tcaaggaaat gattaagaac ggcaatttcg atggcgcaaa acgatttatc gaagaacata
     5821 aagaacaatt aggaccttat aaagagaaag cccaaaatct tttaaaagat gtaaatatcg
     5881 acagtgtcaa aaacaaattt aaaaatctat tcaaataagt aaaactttca cttttgtaaa
     5941 atcaaaggag gacaatgctg ctgcatcgtc ctcctttggt tcttttctga atacttcata
     6001 ctaaaataaa cgtgtcggat cgtaatgata agtcacattg ccgttcagtg aggcttttcc
     6061 caccacatat ttacgattct ccaacttcag ccacgcttct cgaaaagcca ttaattcttc
     6121 tctttttaca tcaatctttt cgatttttcc atcgatcaaa tcttgaataa gttgttcata
     6181 tggattcatt tttttcctcc atctgctgtc aatcaataag ttgtaaaaat aggttcatat
     6241 gcttttttgg tatctagatc ttctctgact ttgactgtcg tataaataat tttttctact
     6301 gggatactat aataacttcc ttcaccatta ttgaatcggt gaacgtcttt ctttgctaaa
     6361 ataccattta ttttattcgt cagtacacgt tgttcgacac tgttacttac aacatagtaa
     6421 atcacccgaa tattattgat caaatgcaaa tcgatttcta acactacatg atccatgaaa
     6481 atcccactcc ttctttcact ttgattatag caagtttctc ctctaatttc aaaatcgata
     6541 cagattttac cggataaatc cacaaaactg cttgcgccat ttcgcacaaa gtgttatttt
     6601 gtatcatgga ctttatttat cggaggcaat agtatgatcg cgatcggtca atttgttttt
     6661 tatattcctt tttttattat gataagcatt cttttctatt acataaaatg gacaaaaaag
     6721 aagttttcag ttttactggc ctctttacct gctgtatatt ttacttatca aattttttct
     6781 tttaggcatt gggaaacaac gtctgtatta ataacacaca tcatcgaatt gacgctttca
     6841 gtaatttttc ttattatttg gatctatttt ttatataaga atcagaattg aatccctcgc
     6901 atgtttaaaa agaaaacgat taaagatgca gcaaatttct gccgatcttt aatcgttttt
     6961 tctattctgt tataccaaaa aaatgtttga atcggtgaag gatcgttcct tgttttaatt
     7021 ctataaattc ttgtgcgatc gggtactttt cttccaaatt ttctttgttc ttttcgatca
     7081 ggcttttcgc ttcttcatat tttttatctg cacatagtgc ttttacttcc tgtaatactt
     7141 ttcctgcttc catttgcctc accctcttgc cattttattg ctttaagttt tatttaagtt
     7201 atacgcatta ttctagtttc atcccaaata accttttttc atttttactc ctcgaactaa
     7261 tcagttttct aaaggctgat tggttcgtct tttttatttt tcctccattt cattctgctc
     7321 ttctgttaat ttatctaatg ctttgattga tgctgcggct cttgcataat catagctgta
     7381 taattccata aatttatcag gcagaacaaa ccacgctgga tgataaccgt tagctgtgac
     7441 agggaatcct ttatcttgtt ggttatctcc atcccatctc agagcaacac ctttttttcc
     7501 tttccacttt actaaagcga tcgagtatcc cttctttgtt tgtgtgccgt cttcaataat
     7561 tgctaaaatt tctatttttt cttttggcgc acggacttct tgaggacgaa ccattttcta
     7621 ttcctccctt atctcattac ttcacgacca acacactgca aggggcatgt gatacgacat
     7681 aagaagcggt cgatccaact aatgcttgtt gtatagcacc ttttcctgtc gctcccataa
     7741 cgatcaaatc aatattttct tgtccaggga tgacattggc aatcaaattt cttggatctc
     7801 cagtttcaac tattgcgtgg atctcagcaa tgccgtactc tttggcatca ttgatttttt
     7861 tcagcatggc tacttctgtt ctttgttttt catctgcata gatttttgaa aaggcatagg
     7921 cactatgggt cagctcaact ttgttgatga ttgacacgat aaataagtgg gcttcattgt
     7981 cctttgctaa gtcaagggct tcagcgaacg ctttgatcga ttgtcttgat ccgtcaactg
     8041 ctaccatgat gttctggtat cttttactca ttggaaccgc ctcctttttc ttttattgta
     8101 acatctcaag caaaaataca aaaaatatac gcaatatgta aataaaatga tgaacaaaag
     8161 caatttcttt caagttattt atacatttct tttctcatca gaacacctgt tctaatccca
     8221 ttatttctgc attatgtgaa attcctcatc atttttcaca tcgagcgaac atcattctgt
     8281 tacaaaaaac gtggatttgt cataaattgt catgcttctt ttacctatga aatattttca
     8341 cgtgttaaag tattcctcgt tgaacacaac aaataaaact ttttaacgaa taaaggagat
     8401 ttttcatgac atcacttaaa actttacttt ttggaacaac tttggctgcc ggcgctgcat
     8461 tcttcatggg aacgactgct catgcggacg aagcttatac cgttcaatca ggtgatactc
     8521 tatcaacgat ttctcaaaaa tacgttggtg ataactcctt gatcaatgcc attgcagaat
     8581 caaattc
//
LOCUS       DAYYHS010000108         8232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000108 DAYYHS010000000
VERSION     DAYYHS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.110"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACHA6A_002621"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACHA6A_002621"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8970751.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACHA6A_002622"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACHA6A_002622"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8970752.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACHA6A_002623"
     CDS             2594..3379
                     /locus_tag="ACHA6A_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8970753.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACHA6A_002624"
     CDS             3392..4243
                     /locus_tag="ACHA6A_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8970754.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACHA6A_002625"
     CDS             complement(4367..4819)
                     /locus_tag="ACHA6A_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970755.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5284..5652)
                     /locus_tag="ACHA6A_002626"
     CDS             complement(5284..5652)
                     /locus_tag="ACHA6A_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8970756.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5802..6284
                     /locus_tag="ACHA6A_002627"
     CDS             5802..6284
                     /locus_tag="ACHA6A_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8970757.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6321..7331
                     /locus_tag="ACHA6A_002628"
     CDS             6321..7331
                     /locus_tag="ACHA6A_002628"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8970758.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7446..8159)
                     /locus_tag="ACHA6A_002629"
     CDS             complement(7446..8159)
                     /locus_tag="ACHA6A_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970759.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1668 g         2442 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga
     5221 acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa
     5281 gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg
     5341 ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata
     5401 tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc
     5461 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
     5521 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
     5581 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg
     5641 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
     5701 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
     5761 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
     5821 aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg
     5881 aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat
     5941 tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg
     6001 atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca
     6061 tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga
     6121 ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta
     6181 gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg
     6241 agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc
     6301 atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg
     6361 gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt
     6421 taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag
     6481 ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag
     6541 atgaaaatat ccaaatgatc tttccgcctt ggggaggaga attattgatt gagattctcg
     6601 attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta
     6661 cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga accaacctta
     6721 ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa
     6781 caagagaagg agaatcgatt ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc
     6841 atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca
     6901 gcggagatgc agtaaggatg aagggacgat tattaggtgg ctgtattgat acgatcagtc
     6961 atttaatcgg taccccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa
     7021 agccgatctt gtggtttcta gaaaattgtg agctatcgtc agttgattta agaagatcac
     7081 ttgttcagat gaaactggca ggatggtttg atggcagctc ggggatcatg ttcggtagaa
     7141 gtgcagcaga ggatttgtta gattataaaa tgaaggatgt atatgaagat ctgtcaaagg
     7201 aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat
     7261 tgatcaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa
     7321 cgtttattta aaaaattttt tcctgccttg gagtttatca aagaccaaca aatctaaata
     7381 gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt ctttttgtat gatctattag
     7441 aaattttaat tatccataac tatcacattg atcagctgtt tcacatcatt tggattacct
     7501 gtaggaatag ctactacaga tgcattatca ataatttctt gagttgtttc tctaatgaac
     7561 gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg
     7621 attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg
     7681 agcataacca catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt
     7741 cctgcaggat aatgaataac atgtagttcg aaatcacccg atgtatctct aaaagtatta
     7801 cctctaattg ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac
     7861 ggatctattg actgagaaga ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt
     7921 gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt
     7981 gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg
     8041 gtttgtttga ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga
     8101 gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata
     8161 taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt
     8221 aaaaccttgt ct
//
LOCUS       DAYYHS010000109         8153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000109 DAYYHS010000000
VERSION     DAYYHS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.111"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            387..575
                     /locus_tag="ACHA6A_002630"
     CDS             387..575
                     /locus_tag="ACHA6A_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8970760.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="ACHA6A_002631"
     CDS             653..1666
                     /locus_tag="ACHA6A_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8970761.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1733..2767)
                     /locus_tag="ACHA6A_002632"
     CDS             complement(1733..2767)
                     /locus_tag="ACHA6A_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8970762.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2882..5107
                     /locus_tag="ACHA6A_002633"
     CDS             2882..5107
                     /locus_tag="ACHA6A_002633"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8970763.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5125..7008
                     /locus_tag="ACHA6A_002634"
     CDS             5125..7008
                     /locus_tag="ACHA6A_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8970764.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6989..7963
                     /locus_tag="ACHA6A_002635"
     CDS             6989..7963
                     /locus_tag="ACHA6A_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8970765.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2644 a         1480 c         1712 g         2317 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1741 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1801 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1861 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1921 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1981 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     2041 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     2101 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     2161 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2221 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2281 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2341 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2401 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2461 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2521 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2581 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2641 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2701 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2761 caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tattgacaaa attgtttagt
     2821 aaacagtata caggaattat aaaaaaaagt aaacggtttc cgttttgttg ggagtgaaat
     2881 gatggatatt acgcatagaa aaacagataa aaatttcatc ttatttgatg aaaagagccg
     2941 agtcttccat ttacgaaata catttctttc ttatctgata aagatagaag aaagcaatgt
     3001 attggcgcat atttattttg gcaaaccagt caagcagtac aaagacaata aaaattatcc
     3061 ccgaagagat cgtggatttt caggaaatgt tcccttaaat cctgaccggt ctctgtcgaa
     3121 agatacacta ccgcaagaat actcttctca tggcagcatg gactttcgaa cgcccgcatc
     3181 gatcattcag cgcaagaatg gatcagactt actggatctg agatatgaca gccattatat
     3241 tacagatggg aagccagaga tcgaaggctt gcctcaaact tatgtgatgg acaagtcaga
     3301 ggcacaaacg ttggtgattt cacttaagga tagagaaaca gcgatttatt tcgatttgtt
     3361 ctataccata tttactgata gagcagtgat cacacgatct gtaaagataa gaaatgaaac
     3421 cggagaaaca atcaaactag aaaaagcggc tagtttccag ctagactttg cccatactag
     3481 gcgatttgat gaagtcattg ctttgccagg agcacatgtc aatgaaagac agatttcacg
     3541 gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt cgaggaacga gcagtcacca
     3601 tatgaataac tttattgctt tagttcatca tcatacgaca gaaaatactg gagaagcgat
     3661 cggacttcaa tttgtttatt ccggcaacca ttctttcgaa cttgaaaaag atcagatcaa
     3721 tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt tcgtgggaat tgaatgcagg
     3781 ccagtctttt caaacacctg aaatgatcct ttcttactca agtcagggat tgaacaaaat
     3841 gagtcagatc caccatgagt tgctacgtga aagaatcgca agaggtcgtc atcaatttgc
     3901 tgaacggccg attctggtca ataactggga agccacttac tttgacttca atagtgaaaa
     3961 aatcaaggcg atcattgatg aagcaaagga gctaggcatc gaaatgttcg tattagatga
     4021 tggctggttt ggaaaaagag atgcggacaa ttcttcatta ggagactggt tcgagtatga
     4081 aggcaagttg acaaatggat tacgtgaaat agcagattat gctcatagca aaggattgaa
     4141 atttggttta tggtttgaac ctgaaatgat ctcggttgat tctgaactgt atcgaacaca
     4201 tcctgatttt cttatgcagg agccaggtag aatgccttct gcttccagat cacaacatgt
     4261 actggatttt acaaggttag atgttcggca gacgattgag aaacaaatga gaaagatttt
     4321 agatacgatt cctcttgact acatcaaatg ggatatgaac cgttcgcttt cagatgtcta
     4381 ttcgattacc cttgacccac agagacaagg agaagtagcg catcgttata tgctcgggct
     4441 ttatgaacta cttgaacatt tgacaactga ttatccggaa atcttatggg aaggctgttc
     4501 tggtggaggt ggacggtttg atgccgggtt tatttattat atgccgcagt catggacaag
     4561 tgataacacg gatgcagtgg agcgtatgaa aatccaatat ggaacaagcc ttgcttatcc
     4621 gatttcgtcc atcacagctc atgtatcagc tgtaccaaat catcagaccg acagatctac
     4681 ttcgttgaaa acaagaggag aaacagctat gagcgctgtt tttggttatg aactggattt
     4741 gacaaagctg tctcctgaag aaaaaaagca agtaaaagac caaattatat cctatcaaac
     4801 gattcgtccg gtgatccagt atggccatta ttatcgtttg gcgagtcctt ttgaagaaaa
     4861 tattgccgca tggatgtttg tgtctcctaa acaagatgaa gctattgtgt ttttagggag
     4921 gatactagct tcagctcaac cagcgttcca tgaagtatat ctgatggggt tagatgatga
     4981 ggcactttat caggaacaga cctcgaagcg gatattttcg ggggccgaat tgatgacagt
     5041 tggactttac ttccccgatt ttcaaggtga tttccaaaca gaactgcttc atttcaaaaa
     5101 gttatgagag agaaggaaaa aagtatgaaa gcaaatataa aatggttaga tgatccacaa
     5161 gtttttagag tgaaccaatc aaatgcacat agcgatcatg tttgttatgc aacaagggaa
     5221 gaagtagaaa ccacatcctc ccttaaacag tctttaaatg gatcatggcg tttttgttat
     5281 tcgccaaatc cttcggtacg accaaaaaca ttttataaaa aagattttga tgcctgttct
     5341 tttgatgaaa tcactgttcc tagtcatatt gaattagcaa attatgacaa gatccattac
     5401 atcaatacga tgtatccgtg ggagggacat gtatttcgcc gtcccccttt cacgacaagc
     5461 cctgagaaag aggcggaagg gatcttttca gctgctaaag acaatccggt tggttcgtat
     5521 ttgacgactt ttcgattaga agaaggtcta aagggaaaac aaatctcgat tcattttgct
     5581 ggtgtagaac aagcaatgtt tatttggttg aatggtcatt ttatcgggta tgccgaagat
     5641 tcgttcactc cgtctgaatt tgatttaaca ccttatttgg aagaagaaaa tattctagct
     5701 gtggaggttt acaaaagaag tacagctgca ttcttggaag atcaggactt tttccgattt
     5761 tttgggatct tcagagatgt cttcttagtt gctcgaggac aaaaccattt agaagatctt
     5821 tggatcaaac ctcgcttaga tgacaaatgt caaaatggca aacttgaagt agatttgtct
     5881 tttgagcaag tagccaaaaa cagtcagctg aatgtagcga tactagatcc tgatgggaag
     5941 gaaatctatc aacagcaata tccgttaaaa gagcaaatga cgattcgcat cgatggattt
     6001 gaaaatgtgg cactgtgggg gcattttcag cccaatttat ataagcttga attggagacg
     6061 atagacgaag aaaaaagagt aacggagtat attgactatt ccttcggttt tcgtaaaatc
     6121 gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga tgattatcaa cggggtaaat
     6181 cgacatgagt ggcatccgaa aaaaggacgc gccatcacta tagaagatat ggaaaaagat
     6241 ttggctatca taaaaaatgc aaagatcaac ggcgtacgga catctcatta ccccaaccag
     6301 atcacttggt attatttatg cgaccaagca ggaatccatg tgatggcgga aacgaacctc
     6361 gaatcccatg gctcatggca aaaaatgggc gcatttgagc cttcttacaa cgtaccaggt
     6421 gcagtacctc agtggcaggc ggctgttctt gatcgagcaa aaacgaattt cgaaacatta
     6481 aaaaaccatc caagtattct tttttggtct cttggaaatg aatcttatgc ggaagaaaac
     6541 attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg atcgacttgt ccattatgaa
     6601 gggaattttc caaatccagc attcgaagat tcgatttcag atgtaaaaag ctgcatgtat
     6661 gcgccaccag aagagatcat tgactacctt gaaaataaag cggaaaaacc gttcatctta
     6721 tgtgaatata tgcatgacat ggggaattcg ttaggtggaa tggattctta catgacgttg
     6781 cttgatcgtt atgaaatgta ccaaggaggc tatatatggg attttaaaga tcaggcgatc
     6841 caagtgactg atccaatcac aggcagagaa gtgatgcgct atggcggtga ctttgacgat
     6901 aaaccaagtg actatgaatt ctcagggaat gggatcgttt ttgcagatgg acaagaaaaa
     6961 cccgccatgc aggaggtaag atattattat gaaaaataca gtaaataaaa gtcatatgga
     7021 tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta ggtgtcaaat cggggaatac
     7081 gcattatatt ttttcttata caagaggcgg actggaatcg ctcaataaaa acggaaaaga
     7141 atggctatac cgggaaacac ttccagtttt ttggcgagca acgaccgata atgacagagg
     7201 tagcggattt ggatttaaat ccggtatgtg gttaggagcc ggattgtatc caaaagtcaa
     7261 agaaatagaa gtactggtgg acaataaaaa gatcgatttg ccgattgctc cagtaaataa
     7321 tcaatattca agtaatgaat acgctgaaac ggttgaaatc atttttacac tggaaatcaa
     7381 cacagtacca aaaacccaag tgacaatttc ctaccaaatc gctggagagg gtaaaatgct
     7441 tgtcaatatg cattattatg gacaaaaagg attaccggaa ttacccgcat tgggtttaag
     7501 gttcattatg ccaacgaaag caaaattttt tgagtatgaa ggcctatcag gggaaacata
     7561 tccggatcgg atggctggtg gtgtcgaagg tatttataaa gtggcaggac tccccgtttc
     7621 cccatacctt gttccccaag aatgcggcat gcatatggaa acgaaatggg tagaactaac
     7681 aagaaatcaa acgttggata atacggataa agatcaagaa gatttttcgt tgcgatttga
     7741 acagcaggat acgccatttg ctttttccgc tttaccattc aaagcagaag aactggaaca
     7801 ggcaacacat atagaagaac taccgccaga aagacgaacg gttcttacga tttttgcaaa
     7861 agtaagagga gtcgggggta tcaatagctg gggagcggat gttgagtcac cttaccgaat
     7921 cagcagcgaa gaggaccatg aaatcggctt tattattcag tagcaatctt tagattatta
     7981 tttgaaaaac ggagtttaca agacaaagct gctttattct atatgtcatg aacaagagag
     8041 taaaaaagtt ctcagatact tttgttcatg actattttct tcaataaaac caattgttat
     8101 ttttctgaaa aacaaagtta actatcagaa aattaaaaaa agtttcataa aaa
//
LOCUS       DAYYHS010000110         7882 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000110 DAYYHS010000000
VERSION     DAYYHS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.112"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACHA6A_002636"
     CDS             complement(220..972)
                     /locus_tag="ACHA6A_002636"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8970766.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACHA6A_002637"
     CDS             1085..2005
                     /locus_tag="ACHA6A_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8970767.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACHA6A_002638"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACHA6A_002638"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8970768.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACHA6A_002639"
     CDS             3311..4789
                     /locus_tag="ACHA6A_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970769.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="ACHA6A_002640"
     CDS             5066..7126
                     /locus_tag="ACHA6A_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8970770.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="ACHA6A_002641"
     CDS             7209..7778
                     /locus_tag="ACHA6A_002641"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8970771.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         2805 a         1318 c         1505 g         2254 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagaaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcagtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaatca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg cc
//
LOCUS       DAYYHS010000111         7719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000111 DAYYHS010000000
VERSION     DAYYHS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.113"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHA6A_002642"
     CDS             <1..107
                     /locus_tag="ACHA6A_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8970772.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHA6A_002643"
     CDS             complement(176..904)
                     /locus_tag="ACHA6A_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8970773.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHA6A_002644"
     CDS             complement(917..2197)
                     /locus_tag="ACHA6A_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8970774.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHA6A_002645"
     CDS             complement(2338..2940)
                     /locus_tag="ACHA6A_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8970775.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHA6A_002646"
     CDS             3185..3391
                     /locus_tag="ACHA6A_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970776.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHA6A_002647"
     CDS             3703..4422
                     /locus_tag="ACHA6A_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8970777.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHA6A_002648"
     CDS             complement(4503..5186)
                     /locus_tag="ACHA6A_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8970778.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHA6A_002649"
     CDS             5405..7591
                     /locus_tag="ACHA6A_002649"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8970779.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2770 a         1300 c         1454 g         2195 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttatt
//
LOCUS       DAYYHS010000112         7712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000112 DAYYHS010000000
VERSION     DAYYHS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.114"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..1045
                     /locus_tag="ACHA6A_002650"
     CDS             122..1045
                     /locus_tag="ACHA6A_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970780.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            1228..3903
                     /locus_tag="ACHA6A_002651"
     CDS             1228..3903
                     /locus_tag="ACHA6A_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8970781.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            4085..4324
                     /locus_tag="ACHA6A_002652"
     CDS             4085..4324
                     /locus_tag="ACHA6A_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970782.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            4340..5713
                     /locus_tag="ACHA6A_002653"
     CDS             4340..5713
                     /locus_tag="ACHA6A_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8970783.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5717..7351
                     /locus_tag="ACHA6A_002654"
     CDS             5717..7351
                     /locus_tag="ACHA6A_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8970784.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7514..>7712
                     /locus_tag="ACHA6A_002655"
     CDS             7514..>7712
                     /locus_tag="ACHA6A_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970785.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2761 a         1175 c         1522 g         2254 t
ORIGIN      
        1 gaccagtttt tttttaagaa taaagtgtga attgacgtat cttttattct tttttctagt
       61 ctaccggtaa attttgagag gagcgtcata tgaaatttat tatgaataag gagatgtatt
      121 aatggctaca aattatgcaa aaaagataaa ttttgatgtg gataaacttt ataaaagttt
      181 gatagttgtt cccaaaaata aatcagaaga tgggaattat gagttagaag ttttgcaatt
      241 taagattgaa caaaagtcca gtgaaagatt tatgagcaat caattagtga aaatcaaaaa
      301 atggctccct atgattgatc aattagtatc taataataag gaagctaaaa atttatgtat
      361 ttttgtctta ttattacatg gaagagttat taacttagtt aatcgaaaaa gtacgactaa
      421 atttgcaaac tcattctggt ttttaaaaaa tattaataaa acagtggaaa agaatggagt
      481 ttataaaggt gattttcatt ttcaaaatgc tgagactatt gaacgagaac atcattattt
      541 cgatttatta gtgaatacag tggtagaatt agcgaaatgg gcaaagtcaa acgaaactta
      601 tagtcaagta cttaaaagaa ttatgagtca agaaacttca tttatctatg agttaagcaa
      661 gtcaaatttg aaatatactt ataatgaaga tgaacttgaa attttagata atccagaaaa
      721 ttcacaatgg attaaagagt tgaagagaga agactttatt gagacactaa gaattctaaa
      781 atcgttattt gatttgaaat tagccaaagc taccatcatg ggaattgaaa gagattcata
      841 ttattgggag gaaattggac tatatgctcc ccagattgaa ggtgaagata aagagaaaag
      901 gatagacaac attattaact atattaatag tttaagtgaa cctttaaaat gtagaggact
      961 tgattttata ggaacattag gatggacaga aacatactat gagaaaatat ataaagaaat
     1021 aattgaacaa tattttccta aataatttaa taaggattga tttatcgtaa aagaaaaaag
     1081 ttgtgatttc caaaatcaca acttttttct tttgtcaaga ttttgagtgg gatatatttt
     1141 tgtagaactg aagatcatga aattatgaga ggttggaggt aatgtagtca tacaaataaa
     1201 aattttttag aaagtgagtg gtaattaatg aaaatgaatc acacttcaat tcagaagcat
     1261 ttacaactgt taaatacact ggggatttct tatgttaatt tgagaacttt ttctacatta
     1321 aacaagaaaa ccgtttctca tattttttct actgcagatt ctcaagcaat tattaactat
     1381 ttagaaaaac agaaattgtc tgaaggtaca gtaaacataa cttataatcc tattaaacaa
     1441 gaggtgatta cacaaaaaaa gcattctgtg agagacaatg atattgaaaa aattcggttt
     1501 gtatttattg atattgaccc taaaaggaaa gagggaagtg caacagatag cgaaaagaaa
     1561 aaggcagaaa atgttatgga acaagtagaa cgttatttga aagaaaagag aatagaatca
     1621 tttgtaaaag ttgatagtgg gaatggctac catatttttc ttcccataaa tgaacaacca
     1681 aataatcacg agacgattct tactattcag aattttcttc aattacttca ccgaagattt
     1741 ggagtagaag atgaagttga tattgatact tctgtttata atccttcaag attatgcaaa
     1801 ttagttggta caccagcggt caaaggaaag tccacaaaag agcgtccgca tcgtttatct
     1861 agtttccttt ctattcccga taacatagat agaagtaatt gttttaaaat tcttgaacag
     1921 atagttgaag aaaattccat gatgtcaacc tctagagtaa aaagccatcc atcttttaga
     1981 gaagtgttac cttttattca agcagactat aaacaatggt tagctcacta tcctgaatta
     2041 acttatacca taaaagaggg cgatcataaa ggagttacct tacttatatt tgacgaatgt
     2101 ccattaagaa aacacacgaa taattctaat ggttcttgtt tgtctgttac ggctgataat
     2161 aagattcgat ttcattgtct gcatgcttcg gataaaaata aagatattca agactttgta
     2221 aaaaaatatc cgttgccaaa acctgtaaat ccttctgtag aatcattaga atccaatcaa
     2281 gaatattatt ttgaccagtt taaactgaat caagaaggag tatttttggt gaaagaaggt
     2341 caagaagaaa aagttgcttg tccgatgttt attaagcaaa tacgcaaaaa tatcgaacaa
     2401 aaagtcatta cacttgttct tgcctattat gtcgatcatt gttgggatga aatagaaata
     2461 gaaggtggat gtttacaaac taataatttt aaacaattag caaaacatgg cttaatcatt
     2521 aaagctagaa gagaagctga agtcgttgac tttttacaga ttcaacggga gaatgctcca
     2581 atcttttttg aacatgaatc tcttggctgg aaaaaagaaa atgaagcaat gatttttcag
     2641 ttacaagatt cttttggacg tgaaattcct tctgtattaa ctaaaaactc atgctaccaa
     2701 ttaacaacta atggtacaga agcggaattt gacgcattgg ttcataagta tgttttagga
     2761 acgaacttag aacttgcttg gtcgattggt attgcttcta ttgtgttagg ctatttaaac
     2821 gttacgaagg ctaatacttt tttgagttta ctaattaacc tacagggcaa gagcacaacg
     2881 ggtaaaacca ctacgttaca ctttattgcc tctttatttg gaaatccaac tacggttctt
     2941 agaaatatga atgctaccaa taaagccatt ataaagctag catcaaacaa tcagggaggt
     3001 gttccactaa tactagatga gttaggagca gcaactcaaa ataactttag ctcgttgatc
     3061 tatcaattag cttcaggaga agaaaggcta agattagatt ctaattgtca gcttatgcca
     3121 caagagactt tttctgtttt gacccttacc tcttctgaag agcgtttgga aaattatttc
     3181 aatgagaaat taactggttt gaaagttcga tatatcgaat tttctgatat caagtttaca
     3241 aaggatgcga atcacgcaga gaaagttaaa agtctttctt ctaatacttt tggttggggt
     3301 ataaagttat ttcttttgaa actctttgaa caagggatta caaaaattga agcaactttt
     3361 gtcgaagctc aaaaaaaact atgggctgtt ttacctgaac attctttgaa gcaacgcatt
     3421 ggtaataatc tagctttgat ttatagtgga gccctcctaa ttcaagatat actgggctat
     3481 cctttacgat tagagcatat acagacggtg cttattaatt cgtacgaaca gttaattgta
     3541 gatactagtt taggaagtga gcaaacggta tttgaccgag tgaaaacatt gttggtgatg
     3601 aatagccata aatttttaac aaacagtgac aacaaacata cgcccagtac gatttgggga
     3661 aaagtatcaa ttcaaaaaaa tggggatatt aaggtaaata tttttcctat cccgtttgag
     3721 gacttgttaa ggcgagaatt tcaagtaaag gatctaacct atctttttag gcaattgctt
     3781 gattcgggta atatgtgtgc agaaaaaggg agaagaacga aacgtattca catgaatcgt
     3841 caagttcttc caacatacga gattctatta ccttcttcac tcaaatcgta ttttcgattg
     3901 taatttagaa taaaccaaag tatgaaagtg aggcaccatg ttatggaaat atttcttatt
     3961 gatttagcaa aacaactgat tacttatctg tatgatagcg agcaaaccca aacggaagta
     4021 aatgaagctt gatctatgct tcatttactt cattcaatca aacatgaaag aaaggaaaat
     4081 tctaatgact aaaaataaaa tgacactaaa agcagaggtt cttctgtata ttcaagaaca
     4141 tttttcaaat caagcctttt ttacaaaacc aatttacttg gatttcgaaa taagaggggt
     4201 atctgctgga tctatcgggg gtactcttca agcgttgaag aacgaaggat atttagaaaa
     4261 tcactttgtg caacgttcgt tcaatagaag agtcgtgaaa aaatggtatt tagttcattc
     4321 atagagaggg gaatcaagca tgcgtgtggc ttgttatgat cgaacgtctg tttatgaaga
     4381 agcgaaaatt agtaatgacg ctcaatatga tgcagtgaaa gagtatgttt tagagaaata
     4441 tgaacaaatt ccccaacgat tttcagacaa aggagaatca ggggaaaaag cggagcgtcc
     4501 agcttttcga cagcttagag cttgggtttg tgcgtcagaa gaagaggggg tgatctatgt
     4561 ttggcgttat gatcgattgg cacgtcaagc caaaatagcc ttagaattta ttgagttgtg
     4621 ccaacaacat caggttcaag tgtattcgat ttctgaacct cttcctgctt ctttctcaca
     4681 cgcgtcaacg aataaactat ttgtgcaatt attgttcact tttgcagaac aacaacgaaa
     4741 cgtcattatt gagaatgttc gtgccggact ttcctataaa aagcaacaac ggcaatattt
     4801 gtcttctgcg gtaccatatg ggtatcgttt agtgaatggg aaaatccaac aagaagtaca
     4861 cgaagcaagg attgttcgct atatttttca actttatttg accaaaaaat atggctacaa
     4921 aaagctttgt caacgattga cgcaacaaaa gcttttcttc cgcgaacgtc cttttcagcc
     4981 ctatcatatt tatagtatcc taaaaaatcc attgtactat ggcgaaatca aaggaggatc
     5041 tttggggaag tatctaggaa cctttgaacc gattctctcg aaagcaacct ttttacaagc
     5101 ccaagagatt cgccaatcaa ggtgtacagc taaaaaagat acgtatctgt acttattacg
     5161 tcaaaaaatt cggtgtccct tctgtggtcg tcatctgagt tcgaagtatc aatggaatac
     5221 aaagaagacg aagacgcttc attattatca ttgtaccgac cgaagctgcc aaggaatttt
     5281 tcttcgggca gaaagggttg agtcagaagt attggaaaag atacaggctt tttttcaata
     5341 cgaaggcgtc tatcaagcct tgattgccca attaacgcaa caattagccg aaaagcgaaa
     5401 agatgaaaaa cgcaaagtaa tccaacaaaa gaaacaagaa gcacaatgga ttaaggcatt
     5461 tgaagagggc aattgttcac tggaagaact caagcaacaa ctgcaagctc ttcaaaagcc
     5521 gatgtctact tcttcgtttc tttcactcca acagtgtgaa gcaaaagtga aagaatggct
     5581 tcaactaaaa gacctctcta gtcgacaatt attatgtgac tatgtttgtc ggattgaggt
     5641 aacaaaggat aaaacagttt gtgggattta cttccgacaa ctgacacaaa atattttaga
     5701 aaggaacgaa tgatctatgg aaatgataca aaccaaacga gtcgcattgt atattcgcgt
     5761 gagtacacaa gaacaagctt tagaaggaca tagtattcaa gcacaagaac aaaatggacg
     5821 acagtttgcc gaacggatgg gctatgaagt ggtagaagtt tatgccgatg aaggaatcag
     5881 tgggaaatcc acaaaaaatc ggttcgccta tcaacgaatg atgaacgatg ctcggaacca
     5941 tcgttttgat ctactagtga tttggaaatt gacccgtctc ggacgcaata tgttagatgt
     6001 cttacaaaca gtcgaggaat tattgcgata tgacgtagga ttacattcga tctcggaaca
     6061 atttgacgtg accacttcga caggtaaatt gatgttacaa ctccttggga gtttcggtga
     6121 atttgaacgc aaccaaattg cggagaatgt tcaaatgtca atgcaaagct tagtgaaaga
     6181 taaaaagcgt tatgcaggag gccgacgttt aggctatgtc agtggtattg acacgaatgg
     6241 tcaaaaacaa ttggtcattg aaccagaaga ggcgaaaatt gttcgtctga tctatgcaaa
     6301 atatttaggc ggagcagggt atcgagcgat tgccaatttt ttgaatcgtc aaggatatca
     6361 aacagtcaaa aagaatgctt tttctaccac ggcagtcaaa gatatcttac gcaataaaat
     6421 ttatgcaggg tatttagaat atgcgcgcta catcaattgg gaaaccaaac gacggaaggg
     6481 gaagaatccg agaccgattt tagtgaaagg aacccatgaa ccgattattt cggaagaaga
     6541 ttatcaaaaa gtacaagaac gccttgcttt agaaggaaaa caacccaaat ggaaccatac
     6601 aggtgaaaat gtattgacag gcttgttacg ttgtccagaa tgtggcgcac cgatggcagc
     6661 gagtaatgtc acaaataccc taaaggatgg cacaaagaaa cggattcgat attattcttg
     6721 ttcagtcttt cggaataaag gagcaagtgt gtgtcatgcc aattcgattc gtgccgaaca
     6781 agcggaaaca tttgtcgccg accgattaaa ggagatcgtt cagctaccag aagttcttcc
     6841 ccgattggtg gcagcgttaa atgaagaaat agtgcgacaa agccaaccat tggaacaaga
     6901 actcgtggtg ttactggaac gaaaggaaga attgaagact aaaattgaaa aatgggaagc
     6961 tgctttggaa gatagtcctg aattgtttcc gatgttaaaa gaccgcttag acgaattgac
     7021 tgaaaaacga cgacagttgc acattcgaga aaatgaaatc ttagggatct ttcaacaagg
     7081 agagccgatt caagtcaaag atgtccaacg gatattgact agtttggatc gttttcttgc
     7141 ccagagtgag aagaagcaaa tcaaagcctt gtatcgtacc tttatagaga aaataacctt
     7201 tgatcctaac cataaggaga tgttggaaat tacgatgaaa ttcacgcatg ccgtggtaca
     7261 acaattgaat gagcaatatc aaatagccgt atccaaaaca aaggatacgg ctatttttgt
     7321 gttaaaaaca ccctttacgt taacgatcta gtctaggtta tatcaaacaa cgttagaggg
     7381 gcgctaacgc ttctctagcg atttgaaatc gttagagaag tagttaggga ggtccttatc
     7441 ataaataggg atatttatta atgaatattt gaaatataat tgtatataag aattaaaaag
     7501 agtggggata gatatgaaaa agatgggtaa gtgtatttta tgtgaaaaat acactgaact
     7561 tactaaagaa catgtaccac ctaaaaaatc agggaatact ggaggtaaaa agaccagaac
     7621 tggtaattta gatgatttcc ttaagagcga ttttacaaaa ggtgattttc cgaaggggat
     7681 taaaaggaaa cctcaaggaa atgtttatta ta
//
LOCUS       DAYYHS010000113         7449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000113 DAYYHS010000000
VERSION     DAYYHS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.115"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..277)
                     /locus_tag="ACHA6A_002656"
     CDS             complement(<1..277)
                     /locus_tag="ACHA6A_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970786.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFF"
     gene            complement(395..655)
                     /locus_tag="ACHA6A_002657"
     CDS             complement(395..655)
                     /locus_tag="ACHA6A_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970787.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(792..1205)
                     /locus_tag="ACHA6A_002658"
     CDS             complement(792..1205)
                     /locus_tag="ACHA6A_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8970788.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1661..2146)
                     /locus_tag="ACHA6A_002659"
     CDS             complement(1661..2146)
                     /locus_tag="ACHA6A_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970789.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2285..2500)
                     /locus_tag="ACHA6A_002660"
     CDS             complement(2285..2500)
                     /locus_tag="ACHA6A_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8970790.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2724..3524
                     /locus_tag="ACHA6A_002661"
     CDS             2724..3524
                     /locus_tag="ACHA6A_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8970791.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3543..5411
                     /locus_tag="ACHA6A_002662"
     CDS             3543..5411
                     /locus_tag="ACHA6A_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8970792.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5404..5895
                     /locus_tag="ACHA6A_002663"
     CDS             5404..5895
                     /locus_tag="ACHA6A_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8970793.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5883..6437
                     /locus_tag="ACHA6A_002664"
     CDS             5883..6437
                     /locus_tag="ACHA6A_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8970794.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6455..>7449
                     /locus_tag="ACHA6A_002665"
     CDS             6455..>7449
                     /locus_tag="ACHA6A_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8970795.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2648 a         1197 c         1423 g         2181 t
ORIGIN      
        1 aaaaaaatag cacaatggca aagatcgtaa gagagatcat aagccccaat gaatgactaa
       61 aaagaaaagc agaatcacat gccatatcat cccatatttc ttcaagaaaa taaataagcg
      121 ggaaaaaggg aaggaatagt agaacatcga tccagcacca tttttttctt cttttgaaat
      181 aacataaaac aataaacatg aacgtgcagg gaagtaaaaa caaagcttta ctcataacta
      241 acaaaatacc agctcctttt atttttttct cccccacatt caaagagtaa caatggtatg
      301 tacttaataa tagaaaaatg gatcaacttg tatgaataac agctgaaaag atctgcacca
      361 aataaaaaac ttccagccaa aactgactgg aagtttattt ttttctgaaa tattctaaca
      421 caaatatgaa aaagatggcc gaagctaata agccgatttc gtagcaaaac aatgagatac
      481 cagagtcgta ataataaaat cctctaatct tatggataaa atagatcatc ggacaagcag
      541 ataagagtaa gtatttgtta ggccttttga ataagtaaaa gaaagcactg gtcattatga
      601 taaatggtat ccaaaacaaa agtttactta agacaatcaa atgatcaact cccatatttt
      661 ttaccaagag agatcataac atctttatcg taaaaacaaa atattttttt gctcattttg
      721 aaaatttaaa catactcgtt cttatccaaa tatataaaaa tcgcccctca ttcatcgaga
      781 ggcgataagt tctaatttaa ctcaattttt tttgtttctt ttggctgata agctgtttta
      841 ggtaatacca atttcagcac accgttttta aaggttccag tgattttttc ttcatcaaca
      901 ttaggaagat agaatgaacg tttgtaagag accgaatgtc tttctttccg tacatagttg
      961 ccatcttctt tatcttctgt atgggactca tgattggcgg agatcgtcaa agtattatct
     1021 gaatattcaa ctgtcgtgtc ttctttgtca cagccaggca tatcagcagt tagtttgtat
     1081 tctttttcat tttcgactaa atctactttt ggataacttg aaacacctaa aaaatcattg
     1141 aatgctggac taaacacgtc attgaaatca gggaacatgt cgcgaatgct aggtacattt
     1201 gccatttttg atcatccttt caataagtat tttttattta tccctattta cgtaataact
     1261 ctattttgaa aaaaatgcaa gtataatggt tagctttgtg aatatttcat ctaatacgat
     1321 acaaaggcta tctttctaat ttgaaaaagg gatcattaaa ataataagaa gaaaatatgt
     1381 caaaaggtaa aaaagtaaat tcttctatcc aaatatagaa aaattgaatc tagaaaaccg
     1441 cccgacaata aatttcatta actcagttga ccgaaaatga agtagatacc aaaaatttat
     1501 caagagagca agtagagaag tgagtatatt cataagagaa atagctgtaa cgaattatta
     1561 ggttaatgca aatgcttatt tatactccgt ataactttct cagaaaaaat gataccaata
     1621 atgacttgct agtactgtaa atccgtataa tatcatctaa tcaaaatggt gttccatttg
     1681 catctgtcat cgtaatcgat ccttcgggtg acactcgata aaaaccaacg cttccagatc
     1741 ctccttggga acggatttgt ttgctggaag ctgtgaacaa ataatctcga tcaatttttt
     1801 gataaaagct aataacaata tcgtcacttt gtaattcgga acggtgggca ataagaaata
     1861 gaagtgcttt gtcagagttt tctaatggaa gagaaggtac agtgctttct tgcatttcag
     1921 atgttgaaga aatgctaaac gactgaatac ttgattggct atgttgcgaa tctacattac
     1981 cagtagaagt attagtaatg gaagtagtag aagtactttt agttgaacca acctcagttg
     2041 ttgaaatggc tgctgaagat tctttaacag aattctttgt tttatatcca caggcagaaa
     2101 aaagtattac agttcccaac aagcctgcgt aaatcccttt atgcatgata gctcctccta
     2161 aaatagatta tctttattca gtatatcact taaacaaaga aaagcagatg gtttaaaatg
     2221 aaaaaacctt cccaataaag ggaaggttca tgaagataat aactttttta ataattatag
     2281 ctttctaata tctttcatca acttttctag taatcattgg gaaaaatttc aagcagtaga
     2341 tcgcacaaat acctacgacg atataaatga tcttcgcaaa aatagttgtt gaaccaccag
     2401 caatcatagc tactaaatca agttcgaaca agcctaccaa cagccagttc aaccctccca
     2461 caatcaataa agttaatgca atagcatcta aagttttcat tagaattctc ctttcttaaa
     2521 tgataaaaca attatatatc aaaaagagaa acattgctta tataacgctc aggaatttta
     2581 gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac attttttagc
     2641 tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg gatttgtaag
     2701 aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga aaagtagtaa
     2761 ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga
     2821 atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa aacctactat
     2881 ctttaaaagt cgatgtgtct tctaaaacgg acatagaaga agggatatat aaagtaatga
     2941 aacatttcaa gacgatcgat ggtctagtca ataatgcagg aataaatatt ccttctcttc
     3001 taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt tttgataaga
     3061 tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga agaatcttgg
     3121 tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg gaaggatcag
     3181 aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact cgttcttggg
     3241 caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg atactggaag
     3301 aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga ggtataagcg
     3361 tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga aggagtggta
     3421 agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca agttatatca
     3481 ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata ggggggacgc
     3541 acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact cacaagaaag
     3601 tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata aaaaaagata
     3661 ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa aacaaacatt
     3721 ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga aaattgaaaa
     3781 cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat aggctgatcg
     3841 aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc agcagaggaa
     3901 cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat gtaaagatta
     3961 caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta cgactacttt
     4021 tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt ctagaagaaa
     4081 aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa atcacccttt
     4141 taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg ttgaagaagc
     4201 ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa ctgatcaatc
     4261 agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt atcagttttc
     4321 ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct gatttgcgct
     4381 tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat ctggatgagt
     4441 ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg gtaatttttc
     4501 gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct ttttcttacc
     4561 aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag atctctaaag
     4621 tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa aaagagcatt
     4681 tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca ttaatgatcc
     4741 aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt tcagaagaac
     4801 aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa
     4861 atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac tcttggttaa
     4921 gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct actgtttttt
     4981 tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc
     5041 ctttgatcag aggctattat gtggatgaag aatttgaaaa aaggatcttg aaaagagaat
     5101 atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc aaccagctat
     5161 ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca gaaacaggag
     5221 gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa gttgagaatg
     5281 aattatttga gctatacgat atcctatttg aactcataaa aatgggaata gatgagttta
     5341 ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa ggagagagac
     5401 ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt gctgcaagca
     5461 gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact gcgaagaaaa
     5521 ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat tgttatgcta
     5581 gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt tacatctttg
     5641 gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat cgatcgttac
     5701 catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa tgccagagaa
     5761 gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt gccttttatt
     5821 gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag gagtgacaaa
     5881 cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa acaggtgcag
     5941 aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg atggttgcaa
     6001 tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc aaattatcag
     6061 cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt atgttgggaa
     6121 atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca agctattatg
     6181 ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt aatcctggag
     6241 aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta aattcgatgg
     6301 agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt ggttggataa
     6361 ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc aaaaaagtag
     6421 agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta agatcgtaag
     6481 aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata aacacaagtt
     6541 catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag aagaactgac
     6601 aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc
     6661 aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag ggataccaac
     6721 catcaatatc gtttccacag gaaaaagtgg cccattagca caatatatca ctgaagatat
     6781 ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg atgataacaa
     6841 agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt ctaaaaagat
     6901 aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa
     6961 agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga tccggacagt
     7021 gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt ctgggatcgg
     7081 taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat taacaatcat
     7141 tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg cagttattag
     7201 ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc ctccccaatt
     7261 agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg
     7321 tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat cagttcttta
     7381 ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa gttttggatt
     7441 atatagctg
//
LOCUS       DAYYHS010000114         7310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000114 DAYYHS010000000
VERSION     DAYYHS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.116"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..383
                     /locus_tag="ACHA6A_002666"
     CDS             <1..383
                     /locus_tag="ACHA6A_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970796.1"
                     /translation="RLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKRKLSH
                     GYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGNICPG
                     FMDQIIENESIESIENYVPVDLPK"
     gene            complement(556..759)
                     /locus_tag="ACHA6A_002667"
                     /pseudo
     CDS             complement(556..759)
                     /locus_tag="ACHA6A_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            725..922
                     /locus_tag="ACHA6A_002668"
     CDS             725..922
                     /locus_tag="ACHA6A_002668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970797.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(965..1540)
                     /locus_tag="ACHA6A_002669"
     CDS             complement(965..1540)
                     /locus_tag="ACHA6A_002669"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8970798.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1695..2221)
                     /locus_tag="ACHA6A_002670"
                     /pseudo
     CDS             complement(1695..2221)
                     /locus_tag="ACHA6A_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2359..2631)
                     /locus_tag="ACHA6A_002671"
     CDS             complement(2359..2631)
                     /locus_tag="ACHA6A_002671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970799.1"
                     /translation="MENRLFELALFCLPLFYKTKYSKQNSSPLPEFFIFIFRKVKKLT
                     TKACFFHSINIFSEKARFISYSSSECTSHKSKKSCEKESTSVPTFS"
     gene            2678..4189
                     /gene="gadC"
                     /locus_tag="ACHA6A_002672"
     CDS             2678..4189
                     /gene="gadC"
                     /locus_tag="ACHA6A_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8970800.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4202..5602
                     /locus_tag="ACHA6A_002673"
     CDS             4202..5602
                     /locus_tag="ACHA6A_002673"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8970801.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5767..7202)
                     /locus_tag="ACHA6A_002674"
                     /pseudo
     CDS             complement(5767..7202)
                     /locus_tag="ACHA6A_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2313 a         1221 c         1304 g         2472 t
ORIGIN      
        1 atagattgac gtgttctaat tgtggtacat tattttacta tgaggaagtt agaaatattg
       61 taaaacatag aaaaaaagaa atgcctatac gttgtccaaa ttgcaatgaa gttattaagc
      121 gaaagttgtc tcatggttat tttgtcagct acgaactata tgaagaaagt aaaataaatc
      181 tgaaaaaaaa accaaaatac tataaaaatt tgaaaactgg agctattata actgaaaaag
      241 aatttaaaaa tttagtgact gaagaagaaa gcaggtttta taatttgtct ggtaatattt
      301 gccctggatt tatggatcag attattgaaa atgagtcaat tgaatcaatt gaaaactatg
      361 ttcctgttga cttaccaaaa tagaaaaaat aatcctgttt ttacagtgaa ttattttaat
      421 tttgattttc aataaaaata tattacaata ataatcgtaa tagctaatga aacaatttct
      481 caatcttttt accactccaa acagggagcg tcaataattt tgtgtaaata actcgtcctt
      541 ctgcaaaata atgggttact cagtaaacat tgaagctaat gtatccgtca cttgttggaa
      601 gcctttatgg cttctattta agaatttttg attgtatgta tcaaagatgg atactaggaa
      661 acgctctagt gattcttcat tttgaaactg ctcttttctg cggctgtatt tcttgatttg
      721 tttattgaaa gattcgattg tagaaccaga aggaatggag aacacttttg aactttacaa
      781 caaaactact gttgataaat taattccaat tttagttcca actgatttaa atggtcatga
      841 tgttttactt cgttttaacg gatttttagg tgaggattat agatacctaa aaatttctga
      901 tcctcaacct ttaacagaat aataaaaaac gtgtaatcaa ttatttgatt acacgttttt
      961 tatttcaatc ttcgttgtag tagtggttgc gacataccga aaggtaatct tcattacctc
     1021 ctactacaat ctgttcacct gaataaactg gaataccatc aacaatacga gcattcatta
     1081 cagccttttt attgcaaaaa tggcaaatag ttttaatttc ctcaatttta tcagcaaatg
     1141 tcaataatgc ttcggatccc ttgaagagat tattttgaaa atcattcttc aatccatagc
     1201 aaattactgg tacttcctca ttcattacga taagtgatag atcaataata ttctctctac
     1261 ttaaaaattg agactcatcg accaaaatac aatcaacttt tccgtactga tcaataatct
     1321 ccactaattc tccgtacaca tccatttcag aagtaattgc aatagctggt ttcgaaacac
     1381 ctatacgact ctcaactttt ccaaatcctg atcgagtatc tattgcgctt gtcattatga
     1441 taactctctt cccttgttct tcataattat gagccacttt cagtagctca atactctttc
     1501 cactattcat tgttccatat ctgtaaaata attgtgccat taaatttatt cccttctttt
     1561 acaattcaat tattccttaa ttccatctgc ttttatgcag ctgctcgttt attccttgat
     1621 atgtctataa catctactat ccctaaaacc aatttataag ccattagaga tggtattcct
     1681 gaacatctct catattgttt tttgagtggt atatcatctg aaaaactgaa ggaatcatga
     1741 aaatagcgac atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca
     1801 gcactttagt catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa
     1861 tcaagtctgc gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct
     1921 ctaatgtgtc attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccattgc
     1981 ggtcgcgcgg aatctggaga tgaagttccc catactcggt cttgaccgta cggtcatagg
     2041 agccgttacg agaattaccc gtgttaaaac aaatgcgatc gtacttttcg taatcgagga
     2101 aagccgttaa ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata
     2161 atccattcaa atcgcctttg ttcgctagag tctttaggat ttctgtagta aaatcgttca
     2221 tatggaagtc ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc
     2281 cttttttcta tttacacaaa atattttaca ctctcaacaa aagtatcttc gattaatact
     2341 tgctcaagtt atgtgcagct acgaaaaagt aggtaccgag gtactttctt tttcgcagct
     2401 ttttttggac ttatgagaag tacattcgct ggaagaatac gatataaaac gagctttttc
     2461 actaaagata ttaatagagt gaaagaaaca agcttttgtt gtcagttttt tcacttttct
     2521 aaaaatgaaa ataaaaaatt ctggaagtgg agaagaattt tgcttgctat actttgtctt
     2581 gtagaaaaga ggtagacaaa ataatgcaag ctcaaataag cgattttcca taaccttggt
     2641 aggaatgaca aattaaatag gaaagggaag taataatatg aataaaaaaa agttatctct
     2701 attcggtttt tttgccctga cagcttccat ggttttgact gtttatgagt atccgacctt
     2761 tgcaacatca caattacatt tggttttctt tcttcttttg ggaggtattt tatggtttct
     2821 accagttgct ttatgtgctg cagaaatggc aacagttgaa ggatggaaag atggtgggat
     2881 tttcagttgg gttagtcaaa ctttaggaga acgttttggt tttgcagcta tttttttcca
     2941 atggtttcaa attacagtag gttttattac aatgatctac ttcatactag gagcgctgtc
     3001 gtatgtaatt aattttccag agcttaatac agatccattt atcaagttta ttggtgtatt
     3061 agtcattttc tgggtactga ctttttcaca gttgggcggg accaatcgta ccgctaaaat
     3121 tgctaaagca ggatttgtca ttggaattgt tattccttcg atcattctct ttggtttagc
     3181 agcagcctac tttatcggcg gtaatccgat tcaaatggct attaatgcga aagcttttat
     3241 tcctgacttt tctcagcttt caactttagt agtatttgta tcttttatat tggcttatat
     3301 gggcgtagaa gcttcagcat cacatattaa tgagctggaa aatccccaaa gaaactaccc
     3361 attggcaatg tttttactag tgattttggc catcgcctta gatgcaatcg gcggtttctc
     3421 agtagcagcg gttattccac aacatgactt gtctctcagt gctggtgtaa tccaaacatt
     3481 tgaaacgctt attttacatt ttaacagtca tttacattgg ttggtaaaag tgattgctct
     3541 catgattgcc tgtggcgtta tgggtgaagt tagttcttgg gtagttggtc catcacgagg
     3601 gatgtttgca gctgctcagc gaggtctgtt accagttttc ttccggaaaa ctaataaaca
     3661 agacgttcca gttccgttga ttatgataca aggaattatc gttactattt gggcagcagt
     3721 cttaactttt ggtggaggag gcaacaattt atcattctta gtagcaatct ccttaactgt
     3781 tgtcatttat ctcgtaggat atttactatt tttcctaggt tactttgtat tagtttttaa
     3841 gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa actgttggca aaacgattat
     3901 cggtggaatt ggttttattt tatcagtgtt cgcattgctt atctcttttg taccaccagc
     3961 ttcaattgca gctagtgagc accgaacata ccaaatcata ctacttatta gctttgtagt
     4021 gactgttctt ttacctttta tcatttatga agtacatgat aaaagtgctc acacaactat
     4081 tgaggaacct actcacgtga aagctgaaga cgttaatccc gctgtttatc cagctgctag
     4141 aggagagcac cacgttacaa aaaatgatga acatatttta aaacattagg aggaattcat
     4201 tatgttatac ggaaaagata atcaagaaga aaaaaactat ttggaaccaa tttttggctc
     4261 tgcaagtgag gatgttgact tgcctaaata taagttaaac aaagaatcca ttgaaccacg
     4321 aattgcttat caattagtac aagacgagat gttggatgaa ggaaatgcgc gattaaactt
     4381 agctactttt tgtcaaacgt atatggaacc tgaagcagtg aaattgatga cccaaacgtt
     4441 agaaaaaaat gcaattgata aatcagaata cccacgaaca acggaaattg aaaaccgctg
     4501 tgtaaatatg attgctgatt tatggcatgc tccaaataat gaaaaattca tgggaacttc
     4561 aacgatcggc tcttcagaag cctgcatgct gggtggtatg gccatgaaat ttgcttggcg
     4621 taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa aaacctaacc tggtgatctc
     4681 ttctggttac caagtttgtt gggaaaaatt ctgtgtatat tgggatgtgg aactgagaga
     4741 agtcccaatg gatgaaaaac atatgtcaat taatctagat actgtcatgg attatgtgga
     4801 tgagtacaca attggtattg taggtattat gggtattact tacactggtc gttatgatga
     4861 tatcaagggt ctgaatgatt tagttgaagc tcacaataaa caaactgact ataaagtata
     4921 cattcatgtt gacgctgcat cgggtggctt ttatgcacca tttactgaac ctgatctagt
     4981 ttgggatttt caattgaaaa atgttatctc aattaattct tcaggtcaca aatatggttt
     5041 ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa caatacttac cagaagaatt
     5101 agtatttaaa gttagttact taggtggaga aatgccaact atggctatca acttctctca
     5161 tagtgcagca caactaattg ggcaatacta caactttgta cgctatggct ttgatggtta
     5221 tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat ttagccaaag agatcgaaaa
     5281 aactggtatt tttgaaatca ttaatgatgg atcagaattg ccagttgtgt gctataagct
     5341 gaaagaagat cccaatcgcg aatggacact atatgattta tctgatcgtc tgttaatgaa
     5401 gggatggcaa gtcccagcct acccattacc taaagacttg gatcaattaa ttattcaacg
     5461 cttagttgtt cgagcagact ttggaatgaa catggctggt gattatgtac aagatatgaa
     5521 ccaagcaatt gaagagttga ataaagctca tattgtttat cataaaaaac aggatgtgaa
     5581 aacgtacggc tttactcact aaaattcttc agagaaatat aaaagaagtt aattaactaa
     5641 taaaaggagt tgtgattacc acacagctcc ttaaatctat ttgaataaat atatgatatt
     5701 catttgttac actctcaatc ttagctgttg ttctattcat cattactctt attgtaagta
     5761 gttcagtcaa atttgcttgt tcaattgatc gataacagta tgaattgttt gaaaagtaat
     5821 cggatatata attgttttct cactacctaa gccttcttgg tataaagaac tatcgcaaag
     5881 tataatccgc tgagtacaaa agaaagtgtc gtctactatt tctctaagtt gtttacaaat
     5941 gatattacta atagaaaagt gttctcccaa aatgtcttct aactgttgtc gatagtataa
     6001 atacctaaag tctgacggag ctactatgaa cacttcaatt ggagatttcc gtttatgctc
     6061 atttaaaaga cttaaactaa tggttagttt acgaatagaa ttctcattcc aacgcagaaa
     6121 taaattgttt tgttttcgcc attgttcaag aatttcatga acttctaagt acaaagcgcg
     6181 ttgctcttca tttaacaagt aagtcttatc aggttgaaat atctgaatat cagcccaaag
     6241 tgagcggata aacgggagaa gtgctttatc aaaaagtatc tgattttctt cagatatatt
     6301 cagagataag ataagttttt gactgagctc aatcaccaaa gggttatctg ctacatggtt
     6361 ttgataaaca acgttcaagt ctttttgtaa caattcaaag ttatcgcata gataatttct
     6421 agagttgaat agaaagtaga tataaaggca ttcatcttcg tcatgacata aagagaaaga
     6481 aagttgctct aatcctttct gtattagctg aaacaaggga gtatgtcggg caactctttt
     6541 ttcttcctga ctaaaatata ttgggttagt cttagatctc tgcaaaccga tagcgatacc
     6601 taataaaata aattgttttt caactgtagg gtaacttctt gaaaaaaatg tttttctaca
     6661 taatcaatca attgttgacc gctttgaata attgatttat ttagctggat caactctgtc
     6721 cgatttgtct catagattat cgttagtaac aaacttcgat aatccttttc aggaatttca
     6781 aaatggccat cagcatttaa gtactgcatt tgtttaaaaa aattaatcaa ctcatttcgt
     6841 aagttgtaaa ctttgcttaa cgaaatgaag gtagtatcag ctatattact ccaattgtgt
     6901 tcccctgata aataatgtcc tagtacattg aaaaagtcag agcgctgata gatgagttga
     6961 gtgagtgcca acctcgaaaa cgaagagtcg aatgcgactg taaagctact tctagatttc
     7021 tccactgaaa taatttgctg atggagtatg tcggtaatgc attccaagtc gttagtgaca
     7081 gtgagtgggc aaacgttcaa ttgatctgct atttcgcgaa tctgaatggt ccctttttcg
     7141 tgtaattgct cacaaatgta aacttgacga aaaatacttt tctccagata ggtttccaac
     7201 atagtactcc tcctagtcat tttatacata actattataa ctctaccaat cggaatagtc
     7261 ctgtaatata ttgaatcaaa aatataaatt ataaaataat tagatggggt
//
LOCUS       DAYYHS010000115         7262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000115 DAYYHS010000000
VERSION     DAYYHS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.117"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..2710
                     /gene="clpB"
                     /locus_tag="ACHA6A_002675"
     CDS             101..2710
                     /gene="clpB"
                     /locus_tag="ACHA6A_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8970802.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2861..3547)
                     /locus_tag="ACHA6A_002676"
     CDS             complement(2861..3547)
                     /locus_tag="ACHA6A_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970803.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3525..4019)
                     /locus_tag="ACHA6A_002677"
     CDS             complement(3525..4019)
                     /locus_tag="ACHA6A_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8970804.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4039..5259)
                     /locus_tag="ACHA6A_002678"
     CDS             complement(4039..5259)
                     /locus_tag="ACHA6A_002678"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8970805.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5406..7241
                     /gene="lepA"
                     /locus_tag="ACHA6A_002679"
     CDS             5406..7241
                     /gene="lepA"
                     /locus_tag="ACHA6A_002679"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8970806.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
BASE COUNT         2394 a         1387 c         1485 g         1996 t
ORIGIN      
        1 atgtgtatat tttgaccaaa attgaccaaa gcgatataat agtttttgta aagatcaaag
       61 aaataagtga tcttaccttt aattaggagg gtatacctgt atgaatattg agaaaatgac
      121 tacgacgctt caagaagcga ttgctgaagc tcaacagatt gcggtcactc gaaaacatca
      181 agatatcgac attgcccatg tatggaaaat ctttttacaa tctaatcatt ttggtcgaaa
      241 tttctatacg gatgccggat tggatgtcga atcttttgaa catgaaattg atcgtctact
      301 ggatgagtat ccagttgttt ctggtggaaa cgtccaatat ggacaaaacc tcagccagaa
      361 tttctttcga ttattgaatg aagcagacca aatacgtgaa agcttcggcg acgagtttct
      421 ttcaacggaa gtcgttattt tagcgttgat gaaattaaaa aattatccgt taactgttta
      481 tttgaataaa aacggattat ctgaaaaaga attgcgtaaa aacatcgaag agatgagagg
      541 aggagaaaga gtgacctcac aaaaccaaga agaacaatac aaagcattag aaaaatacgg
      601 cgttgattta gtacagcaag tcaaaagcgg aaaaatggat ccaatcatcg gtcgtgatga
      661 agaaatccgt gatgtcatcc gtattctttc acgaaaaaca aaaaataatc ctgttctgat
      721 aggagaaccc ggtgtaggga aaacggcaat cgttgaaggg cttgctcaac gtatcgtacg
      781 caaagatgtg cctgaaaatt taaaagataa aacgattttt tcattagata tgggtgcatt
      841 gatcgctgga gcaaaattcc gtggtgaatt tgaagaaaga ctaaaagcag tcttgaaaga
      901 agtcaaaaaa agtgatggac gtatcctctt gttcatcgat gaaatccaca atatcgtagg
      961 ggcaggaaaa acagaaggaa gtatggatgc cggaaatctg ttgaaaccaa tgcttgctcg
     1021 cggtgaatta catttgatcg gtgccacaac tttagatgag taccgtcaat atatggaaaa
     1081 agacaaagcg ttggaacgtc gctttcaaaa ggtattagtc aaagaaccaa cagtggaaga
     1141 tacgatcagt attctgcgtg gattaaaaga acgttttgag atccaccatg gtgttaacat
     1201 ccatgacaac gcattagtag cagcagctac tttatctgac cgttatatta ctgatcggtt
     1261 cttaccggac aaagcaatcg atttgatcga tgaagccagt gcaacgattc gggtagaaat
     1321 gaactctatg ccaacggaat tagatcaagt tactagacgt ttgatgcagt tggaaatcga
     1381 agaagctgca ctgaaaaaag aatcagatga tgcaagtaaa aaacgcttga aaaatttgca
     1441 agaagaatta gctgaacttc gtgaagaagc aaatgcgatg aagatgcagt gggaaacaga
     1501 aaaagaagaa gtcaattctg tttcagcaaa acgtgctgaa atcgataaag caaaacacga
     1561 attagaagat gcagaaaata actatgattt agaacgcgct gctgtattgc gtcatggtac
     1621 gatacctcaa ttagaaaaag aattgaagga attagaagct aaagcaaaag acagcgagat
     1681 taagatggtt caagagtccg ttaccgaaaa tgagattgct caagtcgttg gcagattaac
     1741 gggtatcccc gtaacgaaac ttgtagaagg tgaacgagaa aaactaatta aattgaatga
     1801 aacacttcat aaacgagtga ttggtcaaga tgaagcagtt gatgcggtca gtgacgcggt
     1861 aattcgttca cgggctggat tgcaagaccc aaatcgtcct ctaggttctt tccttttcct
     1921 aggaccaact ggtgtcggaa aaaccgaatt agcgaaagcg ttagctgaga acttgtttga
     1981 ctcagaagat catatggtac ggatcgatat gagtgaatat atggaaaaac atgctgtgtc
     2041 tcgtctagtc ggagctcctc caggctatgt aggttacgaa gaaggtggtc aattaacgga
     2101 agctgtcaga cgaaatcctt atacaatcgt cttgttggat gaaatcgaaa aagcgcatcc
     2161 cgatgtcttc aatatcttgt tacaagtcct agatgacgga cgcttgactg attcaaaagg
     2221 gcgagtagta gatttcaaaa atacggttct gatcatgacc agcaatatcg gttcacagtt
     2281 actgctggaa ggcgtgactg ctgatggaac gattccagaa gcagtggctg aacaagtcaa
     2341 cacgctatta cgtggaaact tcaaaccaga attcttgaac cgaatcgacg acacgatttt
     2401 atttacacca ttgagcctag acaacgtgaa aggaatcgtt gacaaaatgg tggcgcaatt
     2461 ggcacaacgt ctggaacatc aagaaatcct gctgacaatc agtgatgaag ccaaaacgtg
     2521 gattgctgaa aatgcttatg aaccagcata tggtgcaaga ccattgaaac gatttatcac
     2581 taaggaagta gaaacaccac tagctaaaga aatcgttgca ggacatgtta tgcctaaatc
     2641 aaaagtaacg attactttac ttgatggaca acttcatttc aaaacagaag agctagaaga
     2701 aatcgtctaa tcttccattg agactataaa agaaaaaagg ggtctgacac aactagtgtc
     2761 agacctcctt ttttcaaata aacagtgttc cagaagtagt ttcttcgaaa aataagccga
     2821 aatttcttga agctgcccgc ttctgtcaca acctctttta ttaggtattt ttcacttttt
     2881 ctaatttata actttcaaag taacgctcat cttttttact ttgagaccat gcaaacaatc
     2941 ccattaaggc agcacctatc gtatttgtca gcaagtcgcc catcgtatcc atcaatgctg
     3001 ctcgtccgac aaacagtgtg ccatcagaag cagcaaaacg ttgaagattc atgcctaaga
     3061 actggtcgca taagaattcc cagaactccc aaaacacgcc gcaaagtcca gcaaacgcaa
     3121 agccgaataa gaggaacaac caaggactgg tttttgatat ctcagcatct ttcatcaagt
     3181 aaccaatcaa tccatagcca agagctgtca ggaccattgg actcaccgca tgtaagattt
     3241 tgtcccaaaa agagatgata ctgatcaaat gcatccctgt gcctaaaaag acagagatga
     3301 acaagaaaaa ccagtaaaac aaaacgattg ctgctggtat tttaagtttg aaaactttca
     3361 aaatgacttg tggcatgaaa atcaatgcaa tccctaataa agattccacg ataaatgtga
     3421 ttccttgaga agtcgaatat ttttgtgtga taaattcata aaaattatag cagacaacga
     3481 atacaccaaa agcaagtaaa gctatttgca cctttttgat ttctttaatc actgaaagtc
     3541 cctccatcta aaaatctttt tacttctgaa agcaactcaa tttctgaaga aaccactttt
     3601 ccgttcaatt tgatcaaacc tattgtatat aagttcaggt agtgaaattg attttcagct
     3661 gtttcttgta gtgcagcgat ttttttcggg tgggttccgc cttgctgtcg agtatcagta
     3721 tataagccga tcactgggat gcctttcgca taagccacac cgatttccga tgctaccccg
     3781 gcatcgatcg tcagcccatc tagtaaagca atcattaaat cactagccaa cactttttct
     3841 gtgtcagcaa gagcaatcat tcgactgtct gcataagcag ttttgtcatt gattgcttca
     3901 ttttcttgag gaagatagat cgaaagctca gaagagagct ctctgatttc tttaactaac
     3961 tgttggttat ataacaaatc acttttagca aacattggtg cggcaaaata aattttcatc
     4021 taaacatcat cctttttatc aaaaataatc gctaaagtaa gcttccatgt ttggaatgat
     4081 acgctctagt gacttcagtg tttcttcatc tgtttctaac cgttcgattc ttgccaaata
     4141 agaagcacgg cgataattca tcatcaagca agtcaatgtt tggatatcca gtcgaacgga
     4201 ttttcctata ggttcattta agacacggac ttgtccctgt tcatcccatg ttaaagcgaa
     4261 gacaccatta ttccaaggag cgacaggatc ttcaatgatg aaatgaaaag catcagctgt
     4321 tccaacaaac ggaaaccgtt gaaggaattc ttttacatca acgatccgag ccataaaata
     4381 tggttcgatc tgctctttga tctgactatc ttcaatcaaa aatgctaaag gctcattttt
     4441 ataaatatct ccatgtaccc agtagatcat ggaaaaatgt gcggaaataa agttccataa
     4501 gccatttcga gcttcttgat caagatagaa catttctttt acgtgaaata tttcttcagc
     4561 tacccagtag aacagaacac ctcttggttc atggttgcta tcgtagtaga cagcggctgt
     4621 gcgctcatct tcattttcga aacgccaata ctcttcccaa tgaaaatcac tgcggaataa
     4681 agcaccatgg ttttgttggg caaatttgtc ataaacggaa aatacgtctg gatgatcaac
     4741 tggtaatcgc tcaaccatac cacttactgg gatttgttta ggtagctgtg tatctctgat
     4801 tttaaaggaa agtttatcag acatgatttc ccatccttta cgccgatagt aggggatact
     4861 gtaaggatac aaataagaga tccattgctt attcttgcgc atattatcca atgcaacgat
     4921 aattagatct tgcatcaatc catggttagc atattctgga taagttccaa ctccagtcac
     4981 accacccatt ttataacgtg caccgtgaat attgacttca caaggataga tagcgatttg
     5041 agagatcagt ttattttcgt ggaaccatcc gaacactttc gatacttcca aaatcggttt
     5101 tttggaacgg ataaaagctc gtttattctc aaacccgctt tcctcgatgt ctgcttcagt
     5161 cacttggaac acataggaaa gcaattcatt gaattgatcg atatgttctt gagtgaccgg
     5221 ttttaattca agattttctc taaaatcttt ttgatccatt ttttcatctc catttattag
     5281 cttgataaca gtatagcaaa attctacaaa aaaaacttct atagctgact aattaaaaac
     5341 tctttaaatt actgcttttc gttggtataa tggaaagtgc tgattatatg aaaagaggag
     5401 acgctatgga tataaagaaa atgaaagaac gccaagagca gattcgtaat ttttcgatca
     5461 tcgctcatat cgaccatggg aaatctacat tggccgatcg tattttagaa atgacgcaca
     5521 ctgtgacgtc tcgcgaaatg caagatcagc tgttagactc gatggatttg gaacgtgaac
     5581 gtggaatcac gattaaatta aatgctgtag aactcaatta cacagcgaaa aatggagaaa
     5641 catatatctt tcacttgatt gacaccccag ggcacgtcga cttcacctat gaagtctctc
     5701 gaagccttgc tgcctgtgag ggtgccgttt tagtggtcga tgcagcacaa ggaatcgaag
     5761 cacaaacatt ggccaatgtt tatttagcgt tagacaatga tttggagatc ctgccagtca
     5821 tcaataaaat cgacctaccc gcagcagatc ccgaacgtgt ccgtcaagaa atcgaagacg
     5881 taatcggaat cgatgctagt gaagctgtat tagccagtgc caaatcaggg attgggattg
     5941 aagatattct cgagcagatc gtggaatatg ttcctgcacc aagtggagac ttagatgcac
     6001 cgctaaaagc tttgatcttt gactctattt atgatagtta ccgcggtgtt gttctaaatg
     6061 tccggatcac ggacggtatg gtcaaacccg gagacaaaat caaactgatg agtaatggca
     6121 aaacatttga tgttacagag gtcggggtct tctcgccaaa agcagttgct agagactttt
     6181 tgatggtcgg tgatgtcggt tatatcacag caagtatcaa aactgtccaa gatacacgag
     6241 taggggatac agtaacgtta gcagacaatc ctgccgctga agctttacca ggttaccgaa
     6301 aaatgaatcc gatggtttat tgtggtcttt atccaattga tacttctcgt tacaacgatt
     6361 tgcgggaagc tttagaaaaa ctgcaattga atgatgctgc tttacaattc gaaccagaaa
     6421 cctctcaagc attaggtttt gggttccgct gtggattcct cggacttctt catatggatg
     6481 tcgtccaaga acgcttggaa cgggaattca atctagaatt gatcacgact gcaccatccg
     6541 ttatttacca tgttaataaa acggatggta cgactgctac agtagataac ccggcagatt
     6601 ttcctgaacc tgtaacgatt caggatgtag aagaaccatt cgtcaaagca cagatcatgg
     6661 tcccaaatga ttttgtcggt gcagtaatgg aactttctca acgaaaacgg ggagaattca
     6721 ttacgatgga ttatttagat gattaccgag taaatgtcgt ctataatatt ccgctttctg
     6781 aaattgtctt tgactttttc gacaaattga aatcaagcac gaaaggctat gcctcattgg
     6841 attatgaaat gtctggttat caaaaaagca agttagtgaa gatggacatc ttgctgaatg
     6901 gagaaaaagt cgatgcatta agctttatcg ttcaccgaga ctttgcctat gaacgtggaa
     6961 aagcaatcgt tgaaaaactg aaaaaactaa tcccacgcca acaatttgag gtgccaatcc
     7021 aagcagctat tggacaaaaa atcgttgccc gttcagacat caaagccttg cgtaaaaacg
     7081 tcttggctaa atgttatggt ggagatgttt ctcgtaaacg taaactctta gagaaacaaa
     7141 aagaagggaa gaagcggatg aaacaaatcg gctccgtcga agttccgcaa gaagccttca
     7201 tggccgttct gaaaatggat gagcaagata gtgtgaaata acttataatc aatcgaaaaa
     7261 cc
//
LOCUS       DAYYHS010000116         7139 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000116 DAYYHS010000000
VERSION     DAYYHS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.118"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..415
                     /locus_tag="ACHA6A_002680"
     CDS             59..415
                     /locus_tag="ACHA6A_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8970807.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="ACHA6A_002681"
     CDS             405..671
                     /locus_tag="ACHA6A_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8970808.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="ACHA6A_002682"
     CDS             830..1831
                     /locus_tag="ACHA6A_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8970809.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="ACHA6A_002683"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="ACHA6A_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="ACHA6A_002684"
     CDS             3055..3300
                     /locus_tag="ACHA6A_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970810.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="ACHA6A_002685"
     CDS             3398..3619
                     /locus_tag="ACHA6A_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970811.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="ACHA6A_002686"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="ACHA6A_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4761..5300
                     /locus_tag="ACHA6A_002687"
     CDS             4761..5300
                     /locus_tag="ACHA6A_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970812.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            6320..>7139
                     /locus_tag="ACHA6A_002688"
     CDS             6320..>7139
                     /locus_tag="ACHA6A_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8970813.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT         2612 a          979 c         1261 g         2287 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt ttgatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt tttcagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac
     6001 tatattttag gtttcatatt atataaatta ataatatact accatttgtt ggtagtataa
     6061 tttaaaggta ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt
     6121 aataagagtt tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag
     6181 tgatataacg aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga
     6241 tatttagggt agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat
     6301 tttataggag gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa
     6361 taaaggtgat ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt
     6421 gcaggctgaa ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc
     6481 aggtaattcc cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa
     6541 tttggtaatt cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa
     6601 aagaaccaat gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat
     6661 gtctgaaatc tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat
     6721 ttcaaatatg actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga
     6781 atccagatac tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt
     6841 ttcaaaagaa gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct
     6901 tgggtttagt cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct
     6961 aaaagaccgt ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa
     7021 cgatagtatt catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc
     7081 tagaaatatc gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttca
//
LOCUS       DAYYHS010000117         6770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000117 DAYYHS010000000
VERSION     DAYYHS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.119"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..781)
                     /locus_tag="ACHA6A_002689"
     CDS             complement(<1..781)
                     /locus_tag="ACHA6A_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8970814.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSM"
     gene            complement(901..1548)
                     /locus_tag="ACHA6A_002690"
     CDS             complement(901..1548)
                     /locus_tag="ACHA6A_002690"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8970815.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1632..3059)
                     /gene="uxaC"
                     /locus_tag="ACHA6A_002691"
     CDS             complement(1632..3059)
                     /gene="uxaC"
                     /locus_tag="ACHA6A_002691"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8970816.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3133..4212)
                     /gene="uxuA"
                     /locus_tag="ACHA6A_002692"
     CDS             complement(3133..4212)
                     /gene="uxuA"
                     /locus_tag="ACHA6A_002692"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8970817.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4215..5846)
                     /locus_tag="ACHA6A_002693"
     CDS             complement(4215..5846)
                     /locus_tag="ACHA6A_002693"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8970818.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6156..>6770
                     /locus_tag="ACHA6A_002694"
     CDS             6156..>6770
                     /locus_tag="ACHA6A_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8970819.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     RKALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRW
                     LRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLN"
BASE COUNT         2185 a         1359 c          933 g         2293 t
ORIGIN      
        1 tcatactcat aattaataat tgagcaagga gtaagaaaat agccgcacca attatagaaa
       61 gtaatgccct atttttaact aaccctttta aagattttaa caatgaaact ttgtattgag
      121 attggtcaat tcttactcgt tcagtagtta gtttataaca tattaaataa caaataagtg
      181 cacatatcga aaatattccc gcaacaattg taaaagttga tccaccacga actatttgag
      241 cgcctgaatc atctcttgta taaataaata atggtgttaa tacaccaatg accaaacttg
      301 ctaaagtagc tccaacattt cggaatgtcg atagcattgt tctttcttct gtaatagaag
      361 taatagctga tgccattgat ccataaggaa tatttataga tgtgtaaaaa atactccccc
      421 ataatatata cgtaacatac atgtatacta ccttaacgct catagaagct gaaatcatag
      481 aagtttgata cattaaaaaa cttgctaaag cgactggacc actcattctt aaaatccacg
      541 gtttaaactt accctgctta gttgctttcg atctatcaac aatacgcccc atagttacat
      601 cagtaactgc atcgataaat cttgctacta aaaataatgt tccaacggca gctccgctta
      661 tccccaatac ttttgtataa aagatcatta aaaaagaact agcaaaaatg aaagtaaaat
      721 cgtttccaaa atctccaaac atatatccta ttttgtctct tataccaaat tttctttcca
      781 tacaattctc tcccttctgt tgttaagcgt tttcattctg atgaaagtac gtactatact
      841 ctgaagcgta aagagtcagt atttattttt tacatttcag agtattattt gttttttctc
      901 ctaataattc tttatctcat aaaacttatc tatatattta ctagctactc gacttacttc
      961 ttcaaaatca cctttttcaa cagaagccaa taaattccca cctacaccaa ctgcaacaac
     1021 tcctacttca aaccaatctt ctaagttctc tatacttact ccgccagtag gcattatatt
     1081 tacgtgtggt aatggagctt gataggcttt tacaatggat ggaccaaaag cagaacctgg
     1141 gaatagttta atgatatcta cgccagcagt aagtgcctcc tttatctcag taattgtcat
     1201 acaacctggt aaataaggaa tttgatataa attacaaatc tcagctgttt gtcgatcaaa
     1261 agatggacta acgatatatt ctgccccagc cataattgcg atacgagcag taaacgcatc
     1321 taaaaccgta ccggctccga ttattacatc tttttgatca gcatattttt tagcaatttc
     1381 tttgattatt tcttcagcat taggaactgt aaaagttagt tcaatccctt taattccacc
     1441 cgcaataatt gaatttattg ctttaatggc ctcatcctta tttttcccac gtacaaccgc
     1501 aatgactcca gccgcttcta acttacttaa aatttctact tttttcatat ctatctcctt
     1561 cctttatgga tacatacttt gtctagctgt actaacttac aaagaataaa tttaagcttt
     1621 tatttcaagt attattttct acttccgatt tcaaaaaaat taaaataatt atacgcatta
     1681 cgataacaaa tattctccac tatctgacta agatacattt catcatctgg aattcggcct
     1741 gctttaaccc actcgcttag taattgacat aatactcgac gaaaatactc atggcgagga
     1801 tagcttagaa aacttcgaga atcagtcaac atacctgtaa aattcgacaa caaactttgt
     1861 tgtgccatcg tagtcaactg ttgacgcatt ccttcagctg tatcattgaa ccaccaagca
     1921 catcccaatt ggattttttg aatcataccc ccttggaagc tttgcattgc tgttgctagt
     1981 ggaagccaat cagttggatt taatgaatat aaaattgttt ttggtaagca cccactcttt
     2041 tcagcggtat cgaacaatgc tacaacattt tgtgtcaaac tagcttgatc ccccatcgaa
     2101 tcaccaccga catctactcc aatttgttcc tttaatatac ttgaattatt tcttaaagca
     2161 tttaaatgca tttgcatcgt ccaaccatat cttgtatact cagacatcaa atgtaattga
     2221 atacctgtaa caaattgtgc aatttctgat aaatggaaat ccgtttttcc tgctaaagct
     2281 tttattaaca gattgtcgag tattttttca tctgctattt cataaaaata agtattcaaa
     2341 ccgtgatctg ctaatcgaca gccaatttga tgaaaatgat ccactcgttg gctcaatgct
     2401 tgcttcaacc cattaaagtc tttgattggt atagccgctg tttgactaag ttttaaaata
     2461 tagttcgaaa agcctgatga tttgatactc cagaccttat ctggacggaa agtaggtaat
     2521 accttgaaag aattatcctg tttagaaagc tcagtatgat accacaagtc atctgtagga
     2581 tcatccgttg tacagatggc tttcacatcc atcatctcca acaattcttt cgggcgatag
     2641 gaatccgttt gaattttttc gttagcacgt cgccaaattt ctggtgcatt cttctcagaa
     2701 attgtcaaat caatatcaaa gtaacgttgt aattccaaat gagaccattc aaaaatagga
     2761 ttacctggcg ccttctccac tgtttttaca aaagctaaaa atttttcata atcatcacct
     2821 ggaccactga tcaactcttc ttctatacca ttagctcgca tcaatcgcca tttataatga
     2881 tctccagctc ctttatcatt taaccatacc tgagtcaaat tcttgtattg cttattttct
     2941 agcaaatatg ctgggtctaa atgacaatga tagtcgataa ttggttggtg ttttgcaaat
     3001 ttatgaaaaa gtcttttagc tatttcattc tctaataaaa agttcttatc taaaaacata
     3061 tttaaacact cctttccttc cgttagtttt tagaagagaa aatttttcct ctaaataaac
     3121 tcccttatat aattatttac tcgccccctt ttcaagcgct tcccaaatac catttaaata
     3181 aactgcacct agagctcggt catataatcc ataaccaggt ctaccggttt ctccccaaat
     3241 catccgacca tgatctggac gaatatagcc attaaatcca ttatcgtaca ttgtttttaa
     3301 tatcttatac atatctagag aaccatattc tgatttatgc ggcgcttcgt aaaaatcgag
     3361 tccattgtca cttaaatatt tgatattacg aacatgtgca aaaggaatcc gatcccgttg
     3421 acaaaattca gatagaattt catagacatc attatctggt cgttctgcaa tacttccagt
     3481 acataatgta ataccattag aaggagaatc cactacatta cacaaccaat ctaaatcgtc
     3541 acgattttta acaatacgag gtaatccgaa aatggaatat ggtggatcat ctgggtgaac
     3601 tgccatttta atcccaactt cttcacatgt cgggataatt gctttgagaa aatatgcaaa
     3661 gttctctcgt aatttctctt catcaacttc tttataggct tctaataatt gcttaatttg
     3721 tgataaccga tgaggttccc aacccggcaa ttcaaaatta ttagtagaat tttctacttc
     3781 tctaataatt tcctccggat cagtagggat attttttgaa atataggcta acgtagaaga
     3841 cccatcatct aaacgataat ccaaatccga ttttacccaa tcaaatactg gcataaaatt
     3901 ataacagata acctcaatcc caaattctgc caaattgcga atcgtctgtt tataattctc
     3961 aatgtattga tctcgtgttg gtaaaccaat tttgatatca tcatgaacat taacactctc
     4021 aataactttc aaggtaagtc cattggcttc gacttcatct tttaaagatt taattcgttc
     4081 tctcggccaa acctctccgg ctggtatatc cattaatgtt ccaacaattc ctttcattgc
     4141 tgggatctga cgaatttctt gtagtgagat tttttcttca tttgccccat accatctaaa
     4201 agtcatttcc atagttactt tcctccaact tctgttatat atttattgat tgttttacga
     4261 actgcttgag gacctactag catttcgcaa aagatttttt cagttttctt tcctacccca
     4321 acttgatata aatctaaacc aaagatttct ttattttcta aaatttcctg taccgcacaa
     4381 tgaatctttg aaggatcttg ttcacctaat ttcaatactg ataatttatc ctgcaactct
     4441 accaataacg gatcaggact tggcacaaaa ttttcccctt gatcatcgac tgccagtaaa
     4501 taacgtaacc aagcagcaat agcgatagga ataaatgtta gattttctgc atttccgtct
     4561 ttttcgacat actttttaat tgtttcacca tagcgaatcg ctactttttg agaagtatct
     4621 gttgcaattc gttgtggtgt atctggaata tagggattag gcaaacgttt attaattact
     4681 tcatcaataa attgttttgg atcaataatg cctggatttt caactactgg taaaccttct
     4741 aaataaccta ttcctttgat taaaccaacc aaatcatcat cctgcatctc tgctgaaatt
     4801 gattgatatc ctagcagaca acctgttact gccaaagcag tatgaagtgg attcaagctt
     4861 gtcgtaacct tcatgatgtc tgctttatca accgtctctc tatctgtcat aatcacacca
     4921 gccttttcca atttaggccg gccatttgga aaactatctt caataactaa ataatgaact
     4981 acctcagtat tagtgaaagc agcaatattg gtatgttttt ttgtgtgaat gatatctaaa
     5041 tcctcgagcc ccatcatcaa tagatcttcc tttacattct ctgaaggatt tggcgtaatt
     5101 cgatcaatca tactccaagg aaaagtcact ttttctttat cctttaaata agcaataaat
     5161 ttttcagaaa catatccttc tttttgccaa gcttctgcaa tcgtaaaaat actatcttga
     5221 aaacgttgac cattctgtga gaaattatca gtagaaacca tcgcaatcgg caactctcca
     5281 gcattaaatc tgctatataa caaggatgtt acaatactca tcgtatgttg aggattgcta
     5341 ggtccagatt tgatatcagc tttgactacc gaaaagaatt gccctttaga atcttttaaa
     5401 ctgtaacctt tttcagtaat agtaaacgtt gtaaactgta atttaggatc ttcaaaatat
     5461 ttaaccactc tttgatagtc cgctatattc ttcggattgc aatataaact atcagccgtt
     5521 actgccaaaa gtcttttttt gagttcacca tctccactca taacaatttc caaaatgtca
     5581 ttgttatagt catgataagc tttttccact acttcttcat caaatgtttc acacacaact
     5641 actcctgttt ctaattcatt ttgatctgct aagcattgag caatttctgc atgaaatcct
     5701 cgataaagat ttcctccacc aaaatgaagc catactggat gttgctggcc ttcttgctta
     5761 acttctttga catcaaaaca cggcagtaga atctgcattt cattgaattc ttcgatagaa
     5821 ttttgccaat ttttaatcga aaccataatt aacctcctca aactgatatg ttagttttga
     5881 tacattttat attttaaaac ccattgtaac cgttgtcaat ataaaaaggg aataaacttg
     5941 cttttttcta ccatatcaga atgattgtat tttttattca gaaaaataaa atactccttc
     6001 tttttggact gattttatcg aaacttattt tttctttttt tcatgtattc ttttattttt
     6061 ataactagtg tattacttca aaatttgata ttatatggca tgaacagaat taaaatatca
     6121 gttaaaataa aagttaagga gcattgtgta tgacaattaa aactagtctc caaaatcaag
     6181 cttaccaatc tattcgccga caaattatct atgctgactt agaacctgga aaaaaagtat
     6241 cagaaaaaaa attagaagaa acactttcta ttggtcgtac accaatacgt aaagcactga
     6301 ttcaactccg acaacaaggt cttgttgata caattcctca aagcggaaat tatatctcat
     6361 taattgattt agattcggcc aaaaatgctc gatttgtcag agaacatttg gaacgacaaa
     6421 ttatgttaga gtgttgtgcc aaaatgaata accaaaaaaa acggatttta gaaactataa
     6481 tttcagaaca agaaaaagct gttagtcaaa aaaatgctcg tagttttttt cataatgata
     6541 atttattcca taaagcttgt tttgaaattg cagagcgaga agaagtatgg caatggttag
     6601 aaaataacaa tacacactta gaacgatttc gatggctacg agtaaaaacg gaaggattaa
     6661 agtgggaaaa aattatggaa caacattatc aactatttga ggcattaaca aaacaagatc
     6721 cagaagaagc taactttcta acatcgttgc atcttcattt aatgcttaat
//
LOCUS       DAYYHS010000118         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000118 DAYYHS010000000
VERSION     DAYYHS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.120"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHA6A_002695"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHA6A_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA6A_002696"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA6A_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8970820.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHA6A_002697"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHA6A_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHA6A_002698"
     CDS             complement(2143..2373)
                     /locus_tag="ACHA6A_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8970821.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHA6A_002699"
     CDS             complement(2603..3421)
                     /locus_tag="ACHA6A_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8970822.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHA6A_002700"
     CDS             3582..4271
                     /locus_tag="ACHA6A_002700"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8970823.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHA6A_002701"
     CDS             4285..5787
                     /locus_tag="ACHA6A_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8970824.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANRDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHA6A_002702"
     CDS             5800..6276
                     /locus_tag="ACHA6A_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8970825.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHA6A_002703"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHA6A_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2173 a         1032 c         1203 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaataga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAYYHS010000119         6637 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000119 DAYYHS010000000
VERSION     DAYYHS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.121"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHA6A_002704"
     CDS             complement(1273..1938)
                     /locus_tag="ACHA6A_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970826.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="ACHA6A_002705"
     CDS             2118..4100
                     /locus_tag="ACHA6A_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8970827.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHA6A_002706"
     CDS             4104..4742
                     /locus_tag="ACHA6A_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8970828.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHA6A_002707"
     CDS             4770..5198
                     /locus_tag="ACHA6A_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970829.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHA6A_002708"
     CDS             5655..6566
                     /locus_tag="ACHA6A_002708"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8970830.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2284 a         1005 c         1221 g         2127 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaacta
//
LOCUS       DAYYHS010000120         6563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000120 DAYYHS010000000
VERSION     DAYYHS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.122"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA6A_002709"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACHA6A_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHA6A_002710"
     CDS             complement(1878..3080)
                     /locus_tag="ACHA6A_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8970831.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHA6A_002711"
     CDS             complement(3140..3343)
                     /locus_tag="ACHA6A_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8970832.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACHA6A_002712"
     CDS             complement(3485..4549)
                     /locus_tag="ACHA6A_002712"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8970833.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHA6A_002713"
     CDS             complement(4610..4717)
                     /locus_tag="ACHA6A_002713"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970834.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHA6A_002714"
     CDS             4880..5692
                     /locus_tag="ACHA6A_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970835.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHA6A_002715"
     CDS             complement(6066..6257)
                     /locus_tag="ACHA6A_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970836.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1989 a         1329 c         1026 g         2219 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tctgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aag
//
LOCUS       DAYYHS010000121         6387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000121 DAYYHS010000000
VERSION     DAYYHS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.123"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACHA6A_002716"
     CDS             330..1175
                     /locus_tag="ACHA6A_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8970837.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACHA6A_002717"
     CDS             1456..2676
                     /locus_tag="ACHA6A_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8970838.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACHA6A_002718"
     CDS             2673..5393
                     /locus_tag="ACHA6A_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8970839.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACHA6A_002719"
     CDS             5377..6321
                     /locus_tag="ACHA6A_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8970840.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DAYYHS010000122         6325 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000122 DAYYHS010000000
VERSION     DAYYHS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.124"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..958)
                     /gene="pplA"
                     /locus_tag="ACHA6A_002720"
     CDS             complement(23..958)
                     /gene="pplA"
                     /locus_tag="ACHA6A_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8970841.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1260..3194
                     /locus_tag="ACHA6A_002721"
     CDS             1260..3194
                     /locus_tag="ACHA6A_002721"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8970842.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            3651..4067
                     /locus_tag="ACHA6A_002722"
     CDS             3651..4067
                     /locus_tag="ACHA6A_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8970843.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            4081..4647
                     /locus_tag="ACHA6A_002723"
     CDS             4081..4647
                     /locus_tag="ACHA6A_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8970844.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            4670..5656
                     /locus_tag="ACHA6A_002724"
     CDS             4670..5656
                     /locus_tag="ACHA6A_002724"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8970845.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(5811..>6325)
                     /gene="scm"
                     /locus_tag="ACHA6A_002725"
     CDS             complement(5811..>6325)
                     /gene="scm"
                     /locus_tag="ACHA6A_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8970846.1"
                     /translation="SDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDT
                     TSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHA
                     SVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRI
                     TFMSYKKSKR"
BASE COUNT         1844 a         1168 c         1345 g         1968 t
ORIGIN      
        1 ttttattagt atgattatgt gattattttt ctgttacgat attatctact tcgattgttt
       61 gagtgtcgcc tttttcagct gcattcacta attgttgtgc gtacatgatg aatgaatgtg
      121 agctgtgtgt agcacctgta acaacttcta cattagcagg tgagccatct tcttcgccca
      181 ttgctttgac gaattcgtcg ttcagagtag ggatgtaagt ttttggttct gtaccgcttt
      241 ttgctttcat attttcatta tattctgtat catcttgttt tgatttaccg tctttgtcga
      301 tataatcaaa gtttgattca gttactttgc cgccagcaac tgtcatttcg aattgcacac
      361 ggtagccgtt agagtagttc ttttcttcaa gcttgtaagt gccgtctttc aaatcagcac
      421 cattgtcgat ttcgattgta tctgtgttac cagcttgagc agcttggatc aattgttgtg
      481 cgtagttttg gaagctttca gatgagtgag tagctccagt tacgacttcc acaccgcttg
      541 cgctttgtgc ttttaagaat tggtcattca actcaggaat gtatttttct gggtttgtgc
      601 cagattttgc ttccatgttt ttgttgtatt ctgcatcttt tgtttttgat tcgccgtttt
      661 cattgacgtt atcatatttt gattcagtga ttttgccgtc ttttacaacc atttcaaaga
      721 ctgcacggta accatttgaa taattttttt cttctaattt atatgtgccg tcttgcaaat
      781 ctccgccagc tacaactttt gcagatgatt ctgttgtgct ttttgcagtc gaagattgtg
      841 ctgtagatga ttcagaagaa gagtttgagc ttccgttgtc atctgcgccg caagcgccta
      901 acactagagc tgaaaatgct aatacggcaa atccagttac aaaacgtttt ttcttcattt
      961 taatacccca ccattccatt tctagtccac aatttttctt tgtgattcat tatacattct
     1021 aatcttttta gcctataagt caactatttt tgataaataa cccatgtaat atttcaaacg
     1081 ttttctttca gcaaatttcg tgaaaaaacg ctttatttta aaatttgaaa aaaatccaaa
     1141 aggagttaga caaaagaaca gaacatctat ataatatatg gagattgtgt aataagtatc
     1201 aaactaaaag aattatcata tattcattat atagatagtc tataaggagc ggatgatcaa
     1261 tggcaaagca aaatattgtc gttgtaggag caggatatgc cggtgtttca gctacaaaat
     1321 acttggcaaa gaaactgaaa aaagaagatg tagcaattac tctgatcgat cgtcattcgt
     1381 accatactat gatgaccgag ttgcatgaag tagctggcgg acgtgtagaa ccagaagcaa
     1441 tccaatacga tctgcagcat ttgtttgctc gtcaaaagaa tgtacaactt gtcacagaca
     1501 ctgtaatcgg tatcgataaa gaacgaaaaa tcgtcaagac caaattaggt tcctatcctt
     1561 ttgatcagct gatccttgga atgggcggag agccgaatga ctttggtaca cctggtgtca
     1621 aagcacacgg attcaccctt tggtcatttg aagattctgt aaggattcgg gaacatattg
     1681 agaagacagt agcaaaagct gcattggaac cagatgatga actgcgtaaa gccatgctga
     1741 ctttcgttgt ctgtggttct ggatttaccg gtattgaaat gattggggaa ctgatcgact
     1801 ggaaaaatcg cctagccaaa gattataaat tagatccaaa cgaaatcaca ctgatggtcg
     1861 tagaggcaat gccgacgatc ttgaatatgt tggatcgaaa tgatgctgca aaagctgaac
     1921 gctatttgcg taagcacaac gtggaattat tattgaatgc accaatcgta gaagtggctg
     1981 ctgatcatat caaattgaaa gacggacgca acattccgac tcacacactg atttggacag
     2041 ctggtgtcaa aggaacttct gacgctgctg actttggatt agaatcagca cgcggacagc
     2101 ggttgatcgc caatgaatat atgcaggcaa aaggctacga agataaaaac atctatatca
     2161 ttggtgattt agtcttttat gaagaattcc caggaacacc gacaccgcag atcgttcaag
     2221 ctgctgaaca aacaggacac acagccgcag cgaatatcgt ggcttcgatc aaaggcggag
     2281 aaaagcataa atttaaagga aactatcaag gatttatggt ttctattggt gccaaatggg
     2341 gggtagctaa cctatttgat aagatccatt taagcggatt tttagctatc gtaatgaaac
     2401 atatcgtgaa tttgaaatac ttctttgata ttcgttctgg ttattatatg ttccaatatt
     2461 taatgcatga gtttttccat atcaaagatg atcgcaacgt gactcgagga cattcttctc
     2521 gttacggcaa tgtcttatgg agtgtgccac ttcgtatttt ctatggttta gtttggttcg
     2581 ttgaagcttc caaaaaaatc attgggaatg gtgaagtgtt caaaccaagt acttggtttg
     2641 gtgaaggatc atggttcact ggaaacagtg tgttcccttg gacatgggaa tacaatgtaa
     2701 cagatgttgc cagtggtgct tctgctgctg gtgatgcaac cacagctgcc agcggagcag
     2761 gggcaagtgc cggagaagca gcgactcaag ctgcacattt tggtttgagt tatgcatacg
     2821 gtgaagaacc aatggctgtg attgagaagt caccagactg gttcacaaag atcatgcaag
     2881 tgatcatgcc aaacaatgat gtagcaatgt tcttccaaaa attcatggta ttctttgaaa
     2941 ttgccttggc attagcattg attgctggat tattcacatg gctatgcagt gcgacaacga
     3001 ttgtattggt gatcacgttc tgcttatcag gcatgtttta ctgggtaaac atttggttca
     3061 tttttgttgc tttcgcattg atgaacggtt ccggacgtgc gattgggttg gatcgctggg
     3121 taattccatg gctacaacgt acactaggta attggtggta cggtagacca agttcccagt
     3181 acggcaagaa ataaatagat aaagaacgtt atttgaatgc aagatttcaa tagaaagggg
     3241 ctgagacaaa ccgtcacagc ctccttttta tgtaaaatta aaggaataag atgcccgaga
     3301 taagcctcca tgagcaagac acaggtgtca caacctctgt tacaacctct tgttataaac
     3361 ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa cttgaaaagt
     3421 aattttcgta aattccttct tatttcttga agttgcccac ttctgtcaca acctcttgtt
     3481 ataaacggtg ttccacctgc tgcttctcgg taataagccc aaagaattag aaaacatgga
     3541 aagctgtttt ctattcttcg ttcttattac tcaaagcaag acacaggtgt cacaacctct
     3601 tgtttttatg ctaaaattgg gtgagttaat ctggaaaaag gagaacaaaa atgagcctta
     3661 aagaatttgc tgaaaaaagt ctgctcaaac cttgggatgt ttttattatt ttgcttttga
     3721 ctgttctttc ttttttacca gtcatgattt ttggtcttca gcaaaacacc gcaacagcag
     3781 aaaaagaagc ggtactgcgt gtggatggca cagaaatcaa aacattccct ttgatggctg
     3841 gagaagagag caacacatat acttacaaag atgaacacgg agattataac ttgatcgaga
     3901 tagatggcga tcgagtacgg ataaaagaag cagattgcga tgaccagatc tgcgtacgtc
     3961 gaggctgggc aacgaaaaat ggcgaaacca tcgtctgttt gccacataaa ttagtgatcg
     4021 aagtccgttc aacagatgga ggtgacgaag acagtctgat ttattagacc ttcacacacc
     4081 atgagtaaat tacaaaaaat gatttatatt tcgctacttg ttgcacaagg agtgatcatt
     4141 ggtttattgg aaaatatgat cccttatcct ttttcttttg cgcctggtgc aaaactaggc
     4201 ttagccaatc taatcacgat cgttgccatt tttaccatga aaaaacgaga cagcttctta
     4261 ctactttggc tacgactatt cctgactact ttgctaggcg gaacgatttc cacattcctt
     4321 tatagtatga gtggtgcttt gcttagttat tttggaatgt tattggtcaa gcaattagga
     4381 ccaaaacgtg tcagtatcat cggaattagt gcggccggcg gtttcatgca taatgtcgga
     4441 cagcttttga tggcttcttt cattgcacaa tcatggacag tcatgttgta cttgccagtc
     4501 ctttcagttt tagggatttt atcagggatt gcaatcggga tcgcagcgaa ttatctttta
     4561 cgacatgtgc agacacttca acgattccaa aaagattatg agcagggaac caaacaaaaa
     4621 tggagcccaa tgtttaccaa aaaataatcg tcacacaagg agttttttta tgaagctaca
     4681 tccaatgtgg gaaaactatc ctgctttaag taaagaattg acagaaacac taaaacggat
     4741 ggaagaaact gtccgcctga aaaacaaacc tgtcgaacaa gcaatcaaag aaacgatcca
     4801 tgccggcgga aagctgttgc gtcctgccta tcagctattg ttctcacaat ttggaccaga
     4861 gcaagatcga gacaaagcca tcgctttggc agcttctatc gaaatgctcc atacggccac
     4921 actgatccat gatgatatcg ttgatgaagc ggatctgcga cgtggtcagc ctaatatccg
     4981 tagtcagttt ggtaatgtcg tttctgtcta tgcaggagac tatttatttg tctgctgttt
     5041 taaactattg tctgattact ccacttcctt gaaaagtctc caattgaatt ctcgcagtat
     5101 ggaaaaagta ttgaatggcg aattagggca gatggatgat cgctataatt atgaactttc
     5161 tgtaaaagaa tatttggaaa atatttcagg taagacagct gaactgttcc aattaagttg
     5221 ttctgttggc gcttttgaaa gtggaacaag cgagcgcttc gcaaaaaaag caggagatat
     5281 cggactttct atcgggatgg cctttcagat tatcgatgat attttagatt atacaaaaga
     5341 gagccaggaa atcggcaaac ctgtactaga agacatgcgt caaggggtct attctttacc
     5401 gttgatctac tctttgcaaa aaaacaaacc aaagctacta ccttatttag agaagaaagc
     5461 ggcaatgaca gaagaagatg tcgacgccgt acgcaaaatc gttgaacata caggaggcgt
     5521 ggaagaagct aggaagttag cggcctctta cacagaaaag gctttgaagg aaataaagaa
     5581 attacctgcc acctctcttc gaacaaaaga gaatctcttt tcccttaccc aactgatttt
     5641 ggatcgcaaa gactgaatag ggaaaggttc gatacgtaaa taaatttctc agaaggaaat
     5701 ctacgtgttt tttgacaaat tgaattgtag ttcttgtatt tatgataaaa agcaaaaata
     5761 aagagcaacg tccgttagcg acgttgctct tttttatctt gaatattttt ttaccttttc
     5821 gatttcttat aactcataaa agtgattctt aaaccgatta caagtaatat aacgcctgcg
     5881 agtgttatcc aattattcga ttgacttcca gttttaggaa gtgctctttt tgatttgttt
     5941 ccattgtcat tgcttgtttt attagttaag ttaaagttcg tcctgttatc cgaacttgtt
     6001 tttgagaagg agacacttgc atgacgtgta tcgcttgtgg tagacgacgt gtctgtacta
     6061 tcgcttgaag ttgatgaatc tgagctatca cttgtggtag aggatgtatc tgtactgtca
     6121 cttgaagctg acgtgtccgt gctatcactt gtagtggtag atgaatctga gctatcactt
     6181 gtggtagagg tagtatctgt actgtcactt gaagctgacg tgtccgtgct atcacttgtt
     6241 gtggtagatg tatccgtact gtcgcttgaa gctgacgtgt ccgtgctatc acttgtggta
     6301 gaggtagtat ctgtactgtc acttg
//
LOCUS       DAYYHS010000123         6294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000123 DAYYHS010000000
VERSION     DAYYHS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.125"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHA6A_002726"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACHA6A_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8970847.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACHA6A_002727"
     CDS             500..862
                     /locus_tag="ACHA6A_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8970848.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACHA6A_002728"
     CDS             914..2596
                     /locus_tag="ACHA6A_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8970849.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACHA6A_002729"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACHA6A_002729"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8970850.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACHA6A_002730"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACHA6A_002730"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8970851.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACHA6A_002731"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACHA6A_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8970852.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2093 a         1094 c         1435 g         1672 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaa
//
LOCUS       DAYYHS010000124         6221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000124 DAYYHS010000000
VERSION     DAYYHS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.126"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACHA6A_002732"
     CDS             complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACHA6A_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8970853.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1383..1901)
                     /locus_tag="ACHA6A_002733"
     CDS             complement(1383..1901)
                     /locus_tag="ACHA6A_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970854.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1917..2243)
                     /locus_tag="ACHA6A_002734"
     CDS             complement(1917..2243)
                     /locus_tag="ACHA6A_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8970855.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2256..4235)
                     /locus_tag="ACHA6A_002735"
     CDS             complement(2256..4235)
                     /locus_tag="ACHA6A_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8970856.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4256..4570)
                     /locus_tag="ACHA6A_002736"
     CDS             complement(4256..4570)
                     /locus_tag="ACHA6A_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8970857.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4738..5031
                     /locus_tag="ACHA6A_002737"
     CDS             4738..5031
                     /locus_tag="ACHA6A_002737"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8970858.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5109..6221)
                     /locus_tag="ACHA6A_002738"
     CDS             complement(5109..6221)
                     /locus_tag="ACHA6A_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8970859.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
BASE COUNT         1994 a         1270 c         1004 g         1953 t
ORIGIN      
        1 tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc
       61 tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca
      121 gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc
      181 actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg
      241 tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa
      301 gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc
      361 attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga
      421 tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt
      481 tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc
      541 catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc
      601 tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt
      661 caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc
      721 aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc
      781 agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct
      841 cttgacacac atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat
      901 tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc
      961 tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat
     1021 acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt
     1081 ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga
     1141 taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt
     1201 tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa
     1261 catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac
     1321 gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa
     1381 cattagttat tctcctttcc taaatataac gctttttcat attgtttaat acggatatac
     1441 tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa
     1501 ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa
     1561 ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca
     1621 ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc
     1681 tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt
     1741 cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag
     1801 gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc
     1861 tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat
     1921 ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc
     1981 atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca
     2041 tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt
     2101 cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt
     2161 atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt
     2221 tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta
     2281 ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca
     2341 actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc
     2401 gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc
     2461 aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact
     2521 gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct
     2581 cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct
     2641 tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa
     2701 atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt
     2761 atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg
     2821 acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg
     2881 tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta
     2941 atatttttac cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc
     3001 gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc
     3061 atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc
     3121 ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc
     3181 tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac
     3241 aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc
     3301 tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt
     3361 tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga
     3421 aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct
     3481 actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc
     3541 cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt
     3601 aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct
     3661 gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa
     3721 aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga
     3781 ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg
     3841 tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga
     3901 atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa
     3961 aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca
     4021 aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta
     4081 cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca
     4141 gataataaca attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc
     4201 aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata
     4261 tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt
     4321 ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca
     4381 aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat
     4441 tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga
     4501 aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg
     4561 cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt
     4621 tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa
     4681 taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg
     4741 agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa
     4801 gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat
     4861 gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat
     4921 gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt
     4981 aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca
     5041 catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc
     5101 gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc
     5161 accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt
     5221 actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa
     5281 actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc
     5341 cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca
     5401 atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca
     5461 atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca
     5521 acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac
     5581 atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt
     5641 ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta
     5701 aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag
     5761 ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt
     5821 agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt
     5881 gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa
     5941 ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta
     6001 tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg
     6061 aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct
     6121 tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc
     6181 tgttgtcgtc ctttcttttt acttttattt tttacagtca t
//
LOCUS       DAYYHS010000125         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000125 DAYYHS010000000
VERSION     DAYYHS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.127"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHA6A_002739"
     CDS             725..811
                     /locus_tag="ACHA6A_002739"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8970860.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHA6A_002740"
     CDS             complement(932..1621)
                     /locus_tag="ACHA6A_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970861.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHA6A_002741"
     CDS             complement(1628..1906)
                     /locus_tag="ACHA6A_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970862.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHA6A_002742"
     CDS             complement(1866..2780)
                     /locus_tag="ACHA6A_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8970863.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHA6A_002743"
     CDS             complement(2762..3142)
                     /locus_tag="ACHA6A_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8970864.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHA6A_002744"
     CDS             complement(3592..3879)
                     /locus_tag="ACHA6A_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8970865.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHA6A_002745"
     CDS             complement(3899..4123)
                     /locus_tag="ACHA6A_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8970866.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHA6A_002746"
     CDS             complement(4578..4748)
                     /locus_tag="ACHA6A_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970867.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHA6A_002747"
     CDS             complement(4763..5296)
                     /locus_tag="ACHA6A_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8970868.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHA6A_002748"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHA6A_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8970869.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYHS010000126         5904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000126 DAYYHS010000000
VERSION     DAYYHS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.128"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1143)
                     /locus_tag="ACHA6A_002749"
     CDS             complement(139..1143)
                     /locus_tag="ACHA6A_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8970870.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(1214..1510)
                     /locus_tag="ACHA6A_002750"
     CDS             complement(1214..1510)
                     /locus_tag="ACHA6A_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970871.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1503..1796)
                     /locus_tag="ACHA6A_002751"
     CDS             complement(1503..1796)
                     /locus_tag="ACHA6A_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8970872.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1942..2493)
                     /locus_tag="ACHA6A_002752"
     CDS             complement(1942..2493)
                     /locus_tag="ACHA6A_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8970873.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2667..2966)
                     /locus_tag="ACHA6A_002753"
     CDS             complement(2667..2966)
                     /locus_tag="ACHA6A_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8970874.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2993..3301)
                     /locus_tag="ACHA6A_002754"
     CDS             complement(2993..3301)
                     /locus_tag="ACHA6A_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8970875.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3418..4377)
                     /locus_tag="ACHA6A_002755"
     CDS             complement(3418..4377)
                     /locus_tag="ACHA6A_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8970876.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4521..5297
                     /locus_tag="ACHA6A_002756"
     CDS             4521..5297
                     /locus_tag="ACHA6A_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8970877.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(5395..>5904)
                     /locus_tag="ACHA6A_002757"
     CDS             complement(5395..>5904)
                     /locus_tag="ACHA6A_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8970878.1"
                     /translation="TASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILI
                     EQLEWLKKKKIKMAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLK
                     ASEQLRIPVEECLVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLR
                     QEVQVKFGI"
BASE COUNT         1784 a         1083 c          881 g         2156 t
ORIGIN      
        1 ttaaaaataa tagatatttc gaacatcatc actctcatct acttaatttc gcatttttac
       61 tttataaaaa aactgattct aagaaaatta aataattttt ctcaaaatca gtttttcttt
      121 gataaataat attttcaatt atttaatcat ttgaaaattg ttagtcattc caatacgttg
      181 agaaattctt ccatctgtat aaacacggaa ttgttttcca ccagccattg ttgtatttcc
      241 attatcatcc gttacaatat taattgaata gtagcttcca ttttcatctt gcattagatc
      301 tttcatatat tcatttgtgc taattaaatt agtatttgat gcatttttat caggagaatc
      361 atacaaccat aaagctttat tatttagtac agtttcttta gcagcttccc aacttgaaat
      421 tgctgagcta cttgtagatt gtgtcgttgt agttgttgct tgttgagtag attcttctac
      481 tactggagtt tgaggagttt gtccattcac aatatcaatg aaaccttgtt ttaactgttg
      541 gttctgagtt tcattgaagt acatgtagtt tttatcattg ccttgatttt gttgcgttaa
      601 ataaacttta ggttgtccat tttgaaagcc aaagaaatat acatgctgcc ctaaatattc
      661 tccagtttct acatcagaat aaactgctac taaattgaaa tctgcatgac cagttccatc
      721 ttctgaccat tgtacggtaa ctggtgcttc attgattgcc attttccaat ttccatcaat
      781 aactgcactt ggtaaaggcg taccatacag acttacattg ttctgaggat tgtagctttt
      841 atattcttga cctaatgtct ttccccattg tgggacaaaa attgctagtt gagtagattt
      901 ctcactatcc cacaaagtat ttttaacctc ttcagaagaa gattcaatga ttgaactttc
      961 acttgtactt gcttccacag attgtttcat ttctgttgta gtttgttcag ttgattgcgt
     1021 tttacttttt ttagcctcaa ttgttgtatc agtttctaca gtctctttag tcctcgtatt
     1081 ctcagatgta caaccagtga ataaaatccc cattccaatt agtaataaca accatttttt
     1141 cattttattt ccccctttca atttctccct cgcgtactta ttttatcagt caattaatct
     1201 caatggtgtt catttagtta atcctttccc aagaatgtcg atagccagca tgacaacgat
     1261 ctcttccttg agggctttca gatggattaa aactagtaga aatttccctt ccacatcctc
     1321 tacagcgata agttctcatt atttttgggc tattagtatt atttttctta gttagagcta
     1381 tgagtccaac tatgattacc ccaactgtct taattacttt tcctttatct atattcggat
     1441 gcgcaagcat atatttatat aaccaatatc caccgtaaat taaaccacct aatacaatca
     1501 gcttagccat gtactcgtct cctttctttt tgtttaatta ctttttgaat ccatcttgca
     1561 gagaaaccat aaacttctgt caattgacgg atatctacag ttttttcctg ttctatcatg
     1621 ttttcgattc tttttctaac taactcacga tcgtacattt tcattggaaa agaaatctgt
     1681 tgccctcgaa aagcaagata aaattgatag agcaattctg aatccagttc catttcctca
     1741 aaaaagtcat tcatactttt ataagagtta ctcaaacaag tttcatcaat ctccatctac
     1801 acaccccctg ttatttttta cataatgaaa atagcatatt actgggtaca ttgataattc
     1861 atataacatg tatcatcaca tgtattttta aagtcaaaaa gcatgtaaaa ctacttataa
     1921 gtaacataag aaaataaaat gttatttttc ttgaaataat aattgactat ctgccaattg
     1981 ccattcatta cgaacatgaa tacttagtgg gtgggataac ttaccttctg catttatcaa
     2041 tactttggtc atcttttttt gtttcgcact caattgatcg taaatggtct tcgcttgttc
     2101 ataagccact tgactcgtcg ctgtaatcga accacaagca aaaataatgg tgttgatttt
     2161 cttttctgtg aaaacagttg ttaatatttc caaagattgt tcatcatttc cacgaggaaa
     2221 tacccgagga cttttcgctt ttgtatacga aaaaagattc acagcaataa aaccagtaaa
     2281 tcctaattgt cggatattct tttcgattaa taacatactc aaatcattcg taaatggttc
     2341 tgtatctgtt ggacgaatag taatgactac cgctaattta tcttccgctt cttttccttt
     2401 gatcgcccat tgtcgtttga aaacatagcg atgggtatga tgaggatctc gccaaacttc
     2461 tgtttgtgtg gtcactaatt ctttttcaat catctctttt tctcctatgc taatttttgt
     2521 tcaagcaatt caatcaccat tgccgttata agtgtcaata ctagttgaaa atatttcata
     2581 ttctttctcc ttttttcttc agaatttgat gaatcgctgt aaggatatct tctaaacatt
     2641 tcgcaatcat tctcaaataa tcgatcttat tcattcgttc ctccaatctg actccaaacc
     2701 aatcgtcctt ctaccagtaa cccacgaaca aattctcgct ggctggtaat atctgtaatg
     2761 aaataaacta catagtcttt tccatcttct ggtgcgcaat aaatataaag atgtgtcatc
     2821 actaaacgtg gtagagtaac atcttcagga agcataccca tttcaatttc ctctttttcc
     2881 atcgtaacgg attcatagcc ccactcttca ggagtagtga ctagctgtgc acacttttcc
     2941 agtacttcga tagccgtttc taacaagtcc tttcgttcct ttcttttaga tatcagtatt
     3001 cttctggtaa taacatcgtt tgatactcac ccgaatcaat gacccatatt ttttgagaca
     3061 ctgatttcaa acgaagctta ggtgaaatca gatactcttt tcttgcttct ggttcttctt
     3121 gacgatgaac caacttttga tttccttttt ccgtagaaaa ttcaaagatt tgaagatagt
     3181 ctaaatgaaa ttgcttttga acgttttgat cgattagatt ccaacataaa gcttgtaatt
     3241 ccaccggcaa actgtccatt actccctttg tgacataacg ttgtccttta ttttgaaaca
     3301 tttcttttcc ctcctttgcc ataaaaatag cccaatcttc tattaaagaa gattgggctt
     3361 catggatata atatatattt gatattcaat ttgattcatt ttatactatc tgatttgtca
     3421 aattaagcta gacaatatgt ctttatctat gcagctcaaa aatacttcca atggtgttcg
     3481 ataatttaat gattttcttg gaatatggtt tcttttcgag gcaacggatt gaataaaggt
     3541 ttcatccgtg tccctaaagt ccattgtttt aggtaagcca tctcgtctaa gcaagccgtt
     3601 tgagtgctca tttagccctc tttgcgaagg tgttcctgga tctgcgaaaa agatggcaat
     3661 atcgttcgag ttacttatag atcgccagtt ggaaaattct tttccacaat caaatgtcat
     3721 ggatttaaac aagtgtttag gcatggattt gaaccaatca tttaacctgt tttcgatatc
     3781 ttcggctcgt ctgcctgtcg gtttcaaagt aataataacc ttggataaac actcaaccaa
     3841 tgtaataaca gcgctcttgt gtttaactcc cacaatggtg tcgacttcga gatgaccaaa
     3901 ttctttagtc aggtgtggat agagactgtt ccgttcatga atcgttctgc gaaaggcctg
     3961 ttttccacgc ttttcttgat gattattcgg tttacgtttc cccttcatag gtaacgactg
     4021 tgtgtcaaaa aggccttttt taaacatccg atagagtgta cgaacagaac aatcaattgg
     4081 aaactcgcca cggccgataa tcacatcggg tgaccatcct tgagctacct tttcttgaac
     4141 atatgttgat tgttcatcag ataaaacggt tgaacgtcgt ccacaacgag atttattttg
     4201 tttgtattga cggtaataat ctagcacttt ttttccttgt ttgagaaacg aataaacatt
     4261 ataaatggtt tgtctagagc gatttaaact ttgtgcgaca agagcgacag gtttctcgat
     4321 attgaaataa gactctatga ttactagttc gtctgttgta agatgggtgt aagtcatttg
     4381 tactcactcc ttattaattc tttggtcgga actattttga gtgtagcaca aatgatcttt
     4441 ttagttgtct agcttaattt tacaatcgac gttttaataa attgtgatat aatgaagtat
     4501 aactttttag ggagtgtact atgaattttc aagataatag accattatat ttacaagtag
     4561 aagcttctat acgtaacgat attcgtgaaa aaaaatatct tccaggagag caattaccta
     4621 cagaagaaga attatgtaaa atttatggag taagtaagat taccattcga aaagctttta
     4681 aacttttagc agaaagtgga acagttgaac gattaagggg gaaagggacc tttgtaactc
     4741 aaaaaaaaga aagtttaaaa ttagctggag ttcatggatt tagcgattct ctttctaccc
     4801 gaggacataa aattagatat actgttttac attctaaaat tataaaggct gatgagtttt
     4861 tagctaataa attacaaatc aatgtaactg atagagttta caatattaaa cgtttaatgt
     4921 gggaagacgg tgccccgatg ggaattgata attttttcgc atcagaagaa aaatttccaa
     4981 ctttatttga aaaaggtagc caatatactt cattatataa acttctagaa gatgactatg
     5041 atgtcaaagc tacgagctct attatggaaa taaacggttt atctgcaaca cctgagcttt
     5101 ccgacttatt gcaatgtata gtaggagatc cgctatttat tttagaaaag gttggcaaag
     5161 ataaaaataa tcaaccattt cattattctc aatccacagt tcgttgtgat agaattagtt
     5221 atgttattga agtcacagaa aatatcactt tagatacggt gcataaaaat gattcagatc
     5281 ctaaagtaat agattgatat taaaagaaat catacccaaa gcaatcaatt ttgattagat
     5341 ttgggcatga tttttatttt tactaatatt ctctaaatac atcaaaaaac ttatttatat
     5401 tccaaatttc acctgtactt cttgtcgcag atatttaata tcgtaaataa catcatctgc
     5461 tagacttaca tcttgagaaa aacgtttatc tttaattgca agaaccttca tatttgctct
     5521 tttaccagct tttataccat tataagaatc ttctatcacc agacattctt caactggaat
     5581 tctgagctgt tctgaagctt taaggaatat agtaggatta ggtttacttt ctggcaaatc
     5641 tcttccgcta attaaacaat caaaatatga ctctatttta caagtggtaa caacttcatt
     5701 tatagtatcc attggactgg aagaagcaat tgccattttt atttttttct tttttaacca
     5761 ttcaagctgt tcaattagta tattaggaaa aatatccaag taatcaatct tttgcgattt
     5821 ccaatattta gtaaattttc ccttcaattc taaactgtta catccctcat tgtacttttc
     5881 tatatcatca aaaatacttg ctgt
//
LOCUS       DAYYHS010000127         5779 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000127 DAYYHS010000000
VERSION     DAYYHS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.129"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHA6A_002758"
     CDS             <1..813
                     /locus_tag="ACHA6A_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8970879.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHA6A_002759"
     CDS             786..1229
                     /locus_tag="ACHA6A_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970880.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="ACHA6A_002760"
     CDS             2363..3331
                     /locus_tag="ACHA6A_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8970881.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="ACHA6A_002761"
     CDS             3341..3829
                     /locus_tag="ACHA6A_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8970882.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="ACHA6A_002762"
     CDS             4125..4406
                     /locus_tag="ACHA6A_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970883.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="ACHA6A_002763"
     CDS             4408..4824
                     /locus_tag="ACHA6A_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970884.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="ACHA6A_002764"
     CDS             5438..>5779
                     /locus_tag="ACHA6A_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM8970885.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2156 a          982 c          943 g         1698 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag attgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcccttggt
     5281 acaggcgtta cttggtagta tacacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DAYYHS010000128         5727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000128 DAYYHS010000000
VERSION     DAYYHS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.130"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACHA6A_002765"
     CDS             196..921
                     /locus_tag="ACHA6A_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970886.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACHA6A_002766"
     CDS             911..1420
                     /locus_tag="ACHA6A_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970887.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACHA6A_002767"
     CDS             complement(1490..2938)
                     /locus_tag="ACHA6A_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8970888.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACHA6A_002768"
     CDS             complement(2938..3654)
                     /locus_tag="ACHA6A_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8970889.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACHA6A_002769"
     CDS             complement(3635..3985)
                     /locus_tag="ACHA6A_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8970890.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACHA6A_002770"
     CDS             4129..4902
                     /locus_tag="ACHA6A_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8970891.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHA6A_002771"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHA6A_002771"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAYYHS010000129         5698 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000129 DAYYHS010000000
VERSION     DAYYHS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.131"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..967
                     /locus_tag="ACHA6A_002772"
                     /pseudo
     CDS             119..967
                     /locus_tag="ACHA6A_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1320..1514)
                     /locus_tag="ACHA6A_002773"
     CDS             complement(1320..1514)
                     /locus_tag="ACHA6A_002773"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8970892.1"
                     /translation="MAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFIL
                     SILGVLLVFISIKFRKKRNI"
     gene            complement(1581..1646)
                     /locus_tag="ACHA6A_002774"
                     /pseudo
     CDS             complement(1581..1646)
                     /locus_tag="ACHA6A_002774"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
     gene            complement(1649..4441)
                     /locus_tag="ACHA6A_002775"
     CDS             complement(1649..4441)
                     /locus_tag="ACHA6A_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFM8970893.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLALGAY
                     QLVETQAPIGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEILQE
                     LFLSYRTEKEKRYKPV"
     gene            complement(5113..5381)
                     /locus_tag="ACHA6A_002776"
                     /pseudo
     CDS             complement(5113..5381)
                     /locus_tag="ACHA6A_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1594 a         1231 c          920 g         1953 t
ORIGIN      
        1 tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa aaaaacatat
       61 cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga tatgctttat
      121 gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga ttacagaggt
      181 ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc tcttccctgc
      241 tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga aattaatggt
      301 ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc atatgccaat
      361 ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc attggaacgc
      421 tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac ataaaatcac
      481 ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt aggtatcttt
      541 gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt cttccaaaag
      601 aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt tgaggataaa
      661 ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat ttacctacac
      721 aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa gaaatagaat
      781 tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt ctaccaaatg
      841 cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag gcgtaacctt
      901 tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt tctccttatt
      961 gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta tttgtagaaa
     1021 gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact gcgaaactca
     1081 agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact acaaaggttt
     1141 atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt tgggttcaac
     1201 cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg tagtttccat
     1261 tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt tattttagct
     1321 catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac acccaatatt
     1381 gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt atttccactg
     1441 cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt ctcttttgtt
     1501 aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt cgcatccaat
     1561 tcatacccta ttggcgcttg agtttctacc agctgatatg ctcctggtgc taaaccgtca
     1621 attgccagtt ttccatcttc tcctgtagtt agaccggttt gtagcgtttc tccttctcgg
     1681 ttctgtaact caaaaacagc tcctgcagga tttccccgct ttggtcatcg atctttgtca
     1741 acacgacacc gcctggtgta agtcggtttt ctttcgttaa ttctaccacc gctgtctgac
     1801 ttctttcgat ctcaaactcg attggtgtcg catctaattc ataccctatt ggcgcttgag
     1861 tttctaccag ctgatatgct cctagtgcta aaccgtcaat tgccagtttt ccatcttctc
     1921 ctgtagttag accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc
     1981 cttgcaggat ttccccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa
     2041 gtcggttttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga
     2101 ttggtgtcgc atctaattca taccctattg gcgcttgagt ttctaccagc tgatatgctc
     2161 ctggtgctaa accgtcaatt gccagttttc catcttctcc tgtagttaaa ccggtttgta
     2221 gcgtttctcc ttctcggttc tgtaactcaa aaacagctcc ttgaaggact tcaccactgc
     2281 cttcagcatg tttaattagt tcaacaccac ccgtatcaag gatgttttca atggttaacg
     2341 tcacttcttc tatggagtat tcatttataa aaacatcatg taccgtcgca tctaaaagat
     2401 acccttccgg tgcttctgtt tctcggactt gatagctacc tacaggaagc ggttcactta
     2461 aaaattctcc gttttctgcc gttatacctt cttgtatcac ttgtgaatcg cttgataaaa
     2521 tttcaaattt tgctcctgcc agcctttggc cattctcatc agttttaatt actttaatac
     2581 gcccatcgat tttccttgtt cttacagtac tcgtattcaa tcgattgtca ctattcacaa
     2641 gcgttgcaaa gtttaaaatt tgatcgccga ccgcatacgc ctctacattg accttaaaag
     2701 agagggtaaa acttgtcgga ccaccaatcg tattattcgc actgaaacga actattccat
     2761 tattgacttc ggcatcaatg aaagagggaa tatctgatag aaatgacaca aattccatgc
     2821 cttcttccaa aacatcttca ataacaagcg tatttcttgt tccagaaaac atcgagacat
     2881 gcagagtata cgttagttca ttttcattga gattgataac atttttgtcc acactttttc
     2941 ttaaagagat tgccggaaga ttactttcag ggattctcaa gcggtagtct cggctcatta
     3001 cctgattatt gtaggtcata ctcgctcgat tccgagaaaa cccatcctct tgtcttcctt
     3061 cattgaacaa gtcgacatcg gtcacttcaa gagcatattc aataaaatac gattgaccct
     3121 ctgctagatt accaaagtcc acaacaagtg tattcgtttc cttattaaaa gatactttgt
     3181 ccacaaattc ctcggaaaca taatgagttc taacgtttgc ttcacctgga ttcgttactt
     3241 ctgcgatccg aaccccacct gaaataggtt ctctatttcc agtggaactc catgtagtcg
     3301 gattcggtgc caaagttgtt ccttgcggta gtacatcggt aaccactaca ccgtcttttc
     3361 gtgatccttc ataatttacc tgcaaaataa atttattact gttggtcacg tcttgcccgt
     3421 caatcagcag attcccaaga tttccttggc tgtcgatttc tctagagcca tagtataatt
     3481 tcgaaaaaat gggtcttgat gaaggaatag gcgtaattga aaattccgtg tttgctgctc
     3541 gctcagcata agtaagactc acttgaaagt cattatttgg ctcagtaccg cctgattgaa
     3601 agaaagcaac ttcaaacgtg atactgaatg tatacgactg agcggcttca tttaatcctt
     3661 cataatcaaa ggtaaatgga aaaacaacgt ctgtgccttc ttggtaaaat gccagtggct
     3721 ttaaaggtga atcatcagcg acctccactc taatgaactc aattaagcca atcgatgctc
     3781 taggaactcg tacgaccatc tccccatatt cttcaagaat atcgccagcg cctccttgaa
     3841 tgtatccctt taacgtttgt gtatgcctgc tcggcaaagt ggggcgctca gcagagatgc
     3901 cgatgtcata attcaagagc tcctcttgtc tttcaatctg agattcctgc gttatagatg
     3961 gctcagatga tctggcttca ttaaaagatt cacttaagct ttcttctggt atattaaaag
     4021 attcacttgt gctttcttct ggtacattaa aagactcact tgtgctttct tctggtacac
     4081 tgatttctat tgagaccgta tttgattgaa tatcaccgct tcttgcggtc aattcgtatt
     4141 tacctgaatg ctgggaagaa taagtaatca gacgttcgta atcattgagt tgcttcactt
     4201 ccccttcaat caaatcctca ctcttatcaa tgaccaatgc atttagcagg tctggtgttc
     4261 ctctgactcg caactggaac tgctcaccca aagcagcctc ctcttgatca acttcgatcg
     4321 tcagcgaacg ctctggctgt tctgctaaag actcagctag aaccgttatg ttcccaaatt
     4381 gattgatcac aataagcata actagaaaca tacttaacag tttatgaaat ttgttcctca
     4441 ctattactcc cccttataat tttgactaaa caacaaggta cccgataatt ttctaaaaag
     4501 attccccaac tcaaaatggc tgcgttacct ccctcgtatt ttcggttcaa ctcaagcgtt
     4561 gcaatgtaag ctgtaaacaa agctaatgat tgccgattgt cttctgttga ttgattcgat
     4621 agctttgtat gacttttagt tgtccgtgtg gcttaaccat aattatgatt tcacgattcc
     4681 tcctgccagt gccttgcaca agctggagcg taaccctaat agttattttg ttaatgtact
     4741 tatatacttt tatacttttc cattagaaac aatcactaaa tcgttgatac caaggtcaga
     4801 ctctagaatt tcatcagtga gtccctgtat tggttaacct tcatcaatcc caaaaaaata
     4861 tatcaatgct tagcatcaaa atgaatatat agtttagaat acttgggatt catcgataac
     4921 tgatcgctta tttggccata ttatttggca cttggttcaa ctaaacgcat ttaggatgtt
     4981 ccttcattct cgtcatggat gttctcaaac cgtcattact aataaactgt ccaccgaatc
     5041 gatggttcat ttcttgcatt ttccacgtcc aattactctt tttgctcttt atttgggcgt
     5101 agtttcactt tcttaacttg tatcgtcttc catcaactcc ttaatcatct tcttagcttt
     5161 attggctcgg tttccttgta aaaggtatct acaaatagtg acaatctgta ttaaatcttc
     5221 atcattttct gtaatgtcaa taatttctat attctgtacc atatattgca aagggtttca
     5281 ctaagttcat aaccgaagcg aattaaacaa cttttgtata gaataactac cttttctatt
     5341 tttccattgg taattctatc tattaattgg tttaatcctt ttgttgtagt tgattccact
     5401 accaagatga ttctctcgaa ttttaaagct tgaagatgta ttgctatttt tgctaactgg
     5461 cgtatttgca actatttttc ccttctattc ttccttcatt gattcccctc taaaattact
     5521 atccataaaa aattgcataa atgacaatag tatttatatt agtcatactg ctttttaaca
     5581 cagcaaattc cagaaaaaca ttcgttacat atttgtgaat ttttccaaaa aataacattt
     5641 ttataaaacg acgaaaaata aatcactacc agtcaacttc tataatatta ccaaataa
//
LOCUS       DAYYHS010000130         5567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000130 DAYYHS010000000
VERSION     DAYYHS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.133"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      103..269
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            417..974
                     /locus_tag="ACHA6A_002777"
                     /pseudo
     CDS             417..974
                     /locus_tag="ACHA6A_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1032..3071
                     /locus_tag="ACHA6A_002778"
     CDS             1032..3071
                     /locus_tag="ACHA6A_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8970894.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3180..4331)
                     /locus_tag="ACHA6A_002779"
     CDS             complement(3180..4331)
                     /locus_tag="ACHA6A_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8970895.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4485..5015)
                     /locus_tag="ACHA6A_002780"
     CDS             complement(4485..5015)
                     /locus_tag="ACHA6A_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8970896.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5114..5464)
                     /locus_tag="ACHA6A_002781"
     CDS             complement(5114..5464)
                     /locus_tag="ACHA6A_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8970897.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2021 a         1022 c         1042 g         1482 t
ORIGIN      
        1 ttctaaaaaa tattaaagat tgttaaattt cactatttca tttcaaattc cattgcctaa
       61 tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc ttgttaggtg
      121 aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca atgggtaaaa
      181 cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt acgttgtata
      241 gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga aaaggtttct
      301 gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg ctcctcacct
      361 gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt ggtggaggtc
      421 gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca aaactgatct
      481 ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag ataaatgcag
      541 cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat ttcaaatttg
      601 aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt aatgaactgt
      661 ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc gtatatataa
      721 acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga tttcgggaag
      781 acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa gctatctctc
      841 agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg aaattacttg
      901 gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg gatttcctca
      961 aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac aagcaagaga
     1021 aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc gcttatattg
     1081 ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac cagcactatc
     1141 aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc caagctttga
     1201 acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca aataaaagtg
     1261 cattatctga aaaagagatc ttagatcaat accagaatat atacggtgct gctgatgtca
     1321 aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact tacagttttt
     1381 catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct tataaaggaa
     1441 cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg gtttttccag
     1501 aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga gggaatatta
     1561 tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta ggagtcgttc
     1621 caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt gcttcctcct
     1681 tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta caacccgatt
     1741 actttgtccc attgaaaatc attgatggag caacgccaga acttccagct ggagctacca
     1801 ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag ctgattggtt
     1861 acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc agtaatggaa
     1921 aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg acaacaggtg
     1981 agaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc gaacatgaag
     2041 ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa acagcttttg
     2101 acagtctagg aggaaaagct ggatcaactg ttgcgacaac gccaaaaacc ggtgatcttc
     2161 ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg atctcacaag
     2221 aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga tttgcgacag
     2281 gttatgctcc tggatctacg tttaaaataa ttacagcagc aatcggtctc gacaacggca
     2341 ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa gacagttctt
     2401 ggggatcttt taaaacaacg cgtgtcagta gtgatgtatc acaagtagac ttaaaaactg
     2461 ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg ggtgagaaaa
     2521 aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg ccaattagta
     2581 tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg ctagctgata
     2641 ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca atgtattctg
     2701 tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa gagacaaaag
     2761 ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat ctgagagaag
     2821 ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt ccgttggcag
     2881 cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa gagaacagtt
     2941 tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg ttggaaaata
     3001 aagatgatga tgattcagca accaaacgag catcggaact attgcaatac ctcaaccaaa
     3061 attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt atcgtcacag
     3121 tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt ttgatttact
     3181 cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat aagtgtactt
     3241 aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg actttgctgt
     3301 ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc accgaccatc
     3361 aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc aagcaatttg
     3421 gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact aaagtcagaa
     3481 aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc atatcctaaa
     3541 gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc tatcccagca
     3601 aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga cattcctaaa
     3661 caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc tgcaaatgaa
     3721 agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata aattagctgt
     3781 tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag aaaagatagg
     3841 ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc caaaattgtt
     3901 gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga aatactgaca
     3961 gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac ttggaatgct
     4021 ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc aacgaacata
     4081 ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc taaaaacatt
     4141 aataagaaca caccaatctc cgaaaagtca tggactagaa tatcaggttt gacccaattc
     4201 aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc tgggatacct
     4261 aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca taaaatacca
     4321 ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag cataacatac
     4381 attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa agattttttt
     4441 ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg tatagtcatt
     4501 tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa aataaagccg
     4561 aatatttgga atagaagcat caaacttttg aatggattga acaataagac gaatccgcca
     4621 atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt catgctgaag
     4681 aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg caggatagaa
     4741 acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa aagaccactg
     4801 aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc aaaaagattc
     4861 aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg aaaaacagca
     4921 gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct taataaggcg
     4981 tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct ttgacttatt
     5041 ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa ggagaagaat
     5101 atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt gctagctcat
     5161 cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg tcttctactc
     5221 gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca ggggcttccg
     5281 caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct gtttgggaat
     5341 aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct tttctctccg
     5401 cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata cgattttgcg
     5461 ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat ataagaaaaa
     5521 gagaagaaaa agcgattatt caaaaaaaca aaaaggattg tgacaaa
//
LOCUS       DAYYHS010000131         5527 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000131 DAYYHS010000000
VERSION     DAYYHS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.134"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHA6A_002782"
     CDS             complement(<1..300)
                     /locus_tag="ACHA6A_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8970898.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHA6A_002783"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHA6A_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHA6A_002784"
     CDS             complement(1014..1304)
                     /locus_tag="ACHA6A_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970899.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHA6A_002785"
     CDS             1706..1942
                     /locus_tag="ACHA6A_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970900.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHA6A_002786"
     CDS             1949..3901
                     /locus_tag="ACHA6A_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8970901.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHA6A_002787"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHA6A_002787"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8970902.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACHA6A_002788"
     CDS             complement(4797..5489)
                     /locus_tag="ACHA6A_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8970903.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1795 a          965 c          896 g         1871 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttccagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaa
//
LOCUS       DAYYHS010000132         5448 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000132 DAYYHS010000000
VERSION     DAYYHS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.135"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1068
                     /locus_tag="ACHA6A_002789"
     CDS             <1..1068
                     /locus_tag="ACHA6A_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970904.1"
                     /translation="NDYLKNKEIIIPEFDSRNILLALVRYVNWEGYENKNPYVEEACN
                     ELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKSAETKPLFQLVC
                     QGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLNDLSLNEL
                     ILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYGKIVEI
                     LEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFYRTQF
                     HLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKIYPISLK
                     GANIHLYHIAQELHLLKKHIS"
     gene            1289..3052
                     /locus_tag="ACHA6A_002790"
     CDS             1289..3052
                     /locus_tag="ACHA6A_002790"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8970905.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3136..3594
                     /locus_tag="ACHA6A_002791"
     CDS             3136..3594
                     /locus_tag="ACHA6A_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8970906.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3703..4386
                     /locus_tag="ACHA6A_002792"
     CDS             3703..4386
                     /locus_tag="ACHA6A_002792"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8970907.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4533..5147
                     /locus_tag="ACHA6A_002793"
     CDS             4533..5147
                     /locus_tag="ACHA6A_002793"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8970908.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5163..5369
                     /locus_tag="ACHA6A_002794"
     CDS             5163..5369
                     /locus_tag="ACHA6A_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970909.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1952 a          856 c         1113 g         1527 t
ORIGIN      
        1 aatgattatt tgaaaaataa agaaatcatt atcccagaat ttgactcgcg gaacatcttg
       61 ctagctttag tacgttatgt caattgggaa ggctatgaaa ataaaaatcc gtatgtagaa
      121 gaagcttgta acgagttgat tgattatgta gagactcatt tttttaaaag aagatattcg
      181 gaagatgaaa agtcctttat catacgagga atagagatcg caatcggaag aaaggcattt
      241 ccccttggac tgaaagaaga agataagaaa agcgcagaaa cgaagccgtt atttcaatta
      301 gtctgtcaag gattggaaga gcaaaaagga attctagact tacaagaaga agatgtctat
      361 tatatttttt ctttgttcaa cagtagaaat tataccaatg agaacatgga gctgttaaga
      421 aaagatttcg aagttgttta taaaaatttt gttctcaatg acctatcact gaatgaattg
      481 attttacaga ttattagtat cgctgaaatc gacgatgcag aaaattttat tttcaagcaa
      541 tcctttttac aatttgtccg tacgttgtgg gcagatggac aagtattttt accggaaagg
      601 atctatcttc tatcagaaaa agaacaactt ctgtacggaa aaattgtaga aatattggaa
      661 gagtggaaaa agaaaaatca aatccaacta agatggaatg aaaatttcat tcgaaaattc
      721 gtcaaaagtc tttctttgat caacagtgaa caagcgggga gacaggaaat agaaattttt
      781 gttgtttcag aaagttcagt caaacaattt ttttatcgaa cccaatttca tttgtatgcg
      841 gaagaaggca tcgctgaaat aaatcctatt ttatttcgtt cattagaaga attgcctgat
      901 gaatgtctat gtgcgaaaaa aagagtggtt ttgtgcgatg ctgctagcta tcaaaatgga
      961 ttagaaactg aaaagacaaa gatctatcca atctcattaa aaggtgcaaa catccatttg
     1021 tatcatattg ctcaggaact tcacctatta aaaaaacata tttcttgatg agaccttaat
     1081 taggagacct tctacaaagc tttttaattg tctgtagaag gtttttattt gagtcctgat
     1141 tcgtgcgaag atctacttat ataacaagct ttctctgaaa tttttttaaa atgctattga
     1201 cttagagtca actctaagta gtagaataga taaagtgtaa caaatctatt ttcattaaaa
     1261 gaagatagta cggaggagag aatgtattat gagtacagta ttacatgtat tggatgcaat
     1321 caatagcccc gcagatgtca aaaaattatc tgttgaggaa atgaaacagt tggcagaaga
     1381 gatgcgccaa aaaatcttgc aaaaggacag cgatgtaggt gggcacgttg gtccgaatct
     1441 aggtgtagta gaattaacga ttgcgtttca ttatgtattt gattcaccaa aggataaaat
     1501 cgtttgggat gtttcccatc agtcttatgg tcataagctt ttaactggtc gaaaaaatgg
     1561 ttttgaaaaa ggacattacc gtgaagtgag cggatattcc aaccaacatg aaagtaaaca
     1621 tgactatttt acagtggggc acacatctac atctatcagc ttagcaacag gtttagctaa
     1681 agcgagagat ctgaagaaag aatcaggaaa tgtggtagca tttatcggtg acggctccct
     1741 aagtggtggc ttagcttttg aaggattgaa caatggtggt gttttacaat ccaatctaat
     1801 cgtattagta aacgataatg agatgtctat tgatgagaat catggcagtt tatataaaaa
     1861 cttagctgag cttcgtcaaa ccaacggtaa aagcagcaat aacctattta aagcaatggg
     1921 atttgattat cgttacgtgg ataatggaaa cgatctggaa acggtgatcc gtttatttga
     1981 agaagtaaaa gatattgatc atccgattgt acttcacgtc catacggaaa aaggtcgcgg
     2041 ttatcagccg gcaatcgata ataaaatgaa atatcattgg catgtaccat ttgatctgtc
     2101 caccggggag gcaaagcaag taacttctgg agaaacattt aatatgatca tcatggatta
     2161 tttggataag aaaatgcaaa caagtacagc tcctattgcc gcagtcaatg cagcgatacc
     2221 aggcgccttt ggactgaaaa cattccaaga aaaacatcca gatcattatt ttgacgtggg
     2281 gatcgctgaa cagcactcta tcactttcgc ttctgctttg gcttctcagg gagtacgtcc
     2341 agtcgtcttc caatcaagta ctttcttgca acgggcatat gatcaattat cacatgattt
     2401 agcgatcaac gagaatccag tgattatttt agtcggtggc ggaacgatct cagctagtga
     2461 ccgaacacac caaggaacat ttgacattcc ttacttatca agtatcccga acatcaaata
     2521 tcttgctcca gcaacaaaag aggatatgat ctctatgcta gattgggcgt tcgaacaaac
     2581 agcgcagcct gtagcaatcc gaattcctac acatggtgta gaacatggta cagctatcgc
     2641 aaatgattat agtattccag cctatcaaac agtaaataat ggaggaaaag tagcaatttt
     2701 agctttaggc ggttttcttg aattaggaaa agaaacagta aaagctttta gaaaaaaagg
     2761 aatcgaagca acgttgatca atccaggaac gatcacagaa aatgacgtag aaacattaga
     2821 taaattaaaa gaaacacacg aattagtcat cacattagaa gatggtagtt tgtcaggagg
     2881 atttggcgaa aatatcagtc gttattatgg tgattcttca atgaacgtat taaattttgg
     2941 tgcagagaaa gaattcacgg atagcgttcc tgtagggcag ctttatgaac gttatcattt
     3001 aacaccaaaa caaatcgttg aagatagtct gcgtgtcttg aatattcaat aattcattat
     3061 gctcaccatc cagataattg tttatactag tatctggatg gttttttcat tataaaaagc
     3121 aaaggggtgg atgttatgta tcagcaagta gaagaatatg tcaaaaagtt ccaagaaaaa
     3181 tatcctagtt atcaatcaga aaaattaggt ttcttttcat ttggggatac agaaaaaatg
     3241 gcagacgagt tgagcgaact agttgtaaaa aagataaaga ctggtacatc aagtggttac
     3301 gatctgtata aacaagagaa tgagccttta ccaaaagttg gtcaattaga cgtggtcctg
     3361 aatggaaaaa atgaaccagt ttgtatcatc aaaaatataa atgtgaccat cttgcctttt
     3421 agtgaagtca cgaaagagca tgcttggaaa gaaggagaag gggatcggac attagcttat
     3481 tggcggaaag tccatgctga gttttttaaa catgcttata aagaagcgtt gaacatagaa
     3541 tttacagaag agaagctggt cgtatacgaa gaatttgaag taattttctt ttagtaagaa
     3601 aggtctgctg aaagaaatag ctcattattg tctcctgaat agaaaagggt tacattttta
     3661 tatgaagctg tatttttata taacattact ggaggagata ttgtggatac actggaacaa
     3721 atcaaaggcg gagtcgtcgt ttcttgtcaa gctttggaaa acgagccgct acacagttct
     3781 tttattatgg ggagaatggc tttggctgca aaagaaggcg gagcagttgg tattcgagca
     3841 aatacgacag aagatatcaa tgaaatcaag cgaatcgttg atttaccggt aatagggatt
     3901 atcaaaagag attacgatgg gtcagaagtg tttattactg cgacagaaaa agaagtggaa
     3961 gaacttttgg agacgcaagc agaaatcatt gcactggatg caacttctcg taaacgtccg
     4021 acagaaaaaa caactgctga acttgtccaa atgattcacg aacacggtcg gttagctatg
     4081 gcagatattt cgacttttga ggaagcgctt caagcgcaag aagatggttt tgatcttttg
     4141 tctacgactt tagcaggcta tacagaatat tcacgaaaga cgcagacacc tgattttgat
     4201 ttattgaatc aaatcataga acaagtcgat atcccagtaa ttatggaagg acatactgac
     4261 tctcctgaac aagtagagaa agcatatgaa ttaggtgctt tttcagtcgt agttggatct
     4321 attatcaccc gtccgcaatt gatcactaaa agatatgtcg aagccgcaag taaagcaaat
     4381 aactaaaaaa cacgcacggc acagattctt gacaaaaaag aaaaattgat agatagttta
     4441 aagcagattc taagaagatg aaaaaagcat tacttttcat tttgtcttca agatctgctt
     4501 tttaacgaat tttatagaaa gaaggcgaaa ccatgtttga acggagtcct tcagaattga
     4561 cagaacgtga aaattacaag ttgctgattg gttcagtcat ccctagacca gtagcagtag
     4621 taacgactaa atcagcaaaa gatatagtga atatcgcacc cttcagttac ttcaatatcg
     4681 tcagttcgaa tcgacccatc ttatccttgg cgatccagag aaaacaagat gcagtaaaag
     4741 acacagcaaa gaatattctt gagacaagag aagctgttat ccatatcctt gatgaggaca
     4801 acgtagaaga agtaaataaa acagcagcca atctgcctaa tgaggaaagc gaactgtacc
     4861 tgactgactt gactttaact gattcagtaa tcatttcagt accagcattg acagaagcaa
     4921 aagttcgttt tgaaacatcc ttatatgaac atgtcgagat acggaataaa aataaagtaa
     4981 cagctgattt atttttgcta gaagtacaga aatatcattt aaatgaaaca gtctatgatg
     5041 ataagacagg aaggatcgat ccaagaaaat tgaatgctgt aagccgtttg gcaggaaatg
     5101 attatgcagc aataggagaa attttcacga ttgcacgtcc cgaataaaaa ggagcactag
     5161 ttatgttaaa aataagctat aaaccatcga cagattcaaa agaaatgaaa aaagaatatg
     5221 aaacagtgaa tgattttctg caagggcagt acttagaagt tccgccattg caagaccatt
     5281 ttgtcgttac tactgttact ttagatggta aggaaataga aatgccagat caaacgatta
     5341 gcggattgtt taattacttt aacaaataaa tatttattcc gaaaagtgaa aaaacatata
     5401 aaaaaacaca taaaactaaa aaattatcaa agaatcctgg tgcaactg
//
LOCUS       DAYYHS010000133         5285 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000133 DAYYHS010000000
VERSION     DAYYHS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.136"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACHA6A_002795"
     CDS             32..388
                     /locus_tag="ACHA6A_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFM8970910.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACHA6A_002796"
     CDS             404..754
                     /locus_tag="ACHA6A_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFM8970911.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACHA6A_002797"
     CDS             783..1562
                     /locus_tag="ACHA6A_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFM8970912.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACHA6A_002798"
     CDS             1573..2235
                     /locus_tag="ACHA6A_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFM8970913.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACHA6A_002799"
     CDS             2254..3360
                     /locus_tag="ACHA6A_002799"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFM8970914.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="ACHA6A_002800"
     CDS             3350..4441
                     /locus_tag="ACHA6A_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFM8970915.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="ACHA6A_002801"
     CDS             4454..5194
                     /locus_tag="ACHA6A_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFM8970916.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1698 a          865 c         1258 g         1464 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taata
//
LOCUS       DAYYHS010000134         5251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000134 DAYYHS010000000
VERSION     DAYYHS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.137"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..732
                     /locus_tag="ACHA6A_002802"
     CDS             <1..732
                     /locus_tag="ACHA6A_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8970917.1"
                     /translation="TNSEIANKLGVSEMTIRRDVTELAKKNKVKKLYGGVERKEPYNR
                     ELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGTTILYALPFIQKKNLFVITNS
                     LIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSFESLNVDISFAATNGIFDNNV
                     TTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTFRKLTDFDYLITDNHLSEQDF
                     NHYSKYLTILKEPKNDTDNHDEPIH"
     gene            698..1651
                     /locus_tag="ACHA6A_002803"
     CDS             698..1651
                     /locus_tag="ACHA6A_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFM8970918.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2049..3023)
                     /locus_tag="ACHA6A_002804"
     CDS             complement(2049..3023)
                     /locus_tag="ACHA6A_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8970919.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3138..3572
                     /locus_tag="ACHA6A_002805"
     CDS             3138..3572
                     /locus_tag="ACHA6A_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8970920.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3697..4719)
                     /locus_tag="ACHA6A_002806"
     CDS             complement(3697..4719)
                     /locus_tag="ACHA6A_002806"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFM8970921.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5111..>5251
                     /locus_tag="ACHA6A_002807"
     CDS             5111..>5251
                     /locus_tag="ACHA6A_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFM8970922.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRA"
BASE COUNT         1644 a         1012 c          940 g         1655 t
ORIGIN      
        1 actaattcag aaatcgctaa caaattaggc gtatcagaaa tgaccatacg ccgagatgtc
       61 actgaattag caaaaaaaaa taaagtaaaa aagctatatg gtggcgttga acgaaaggaa
      121 ccttataatc gagaactatc gactcaagaa aagattgata cgaatgtagc cgaaaaaaaa
      181 tatattggtc aagtcatgaa cagtatgatt cccgataatt ctactattta tgtcggtgct
      241 ggaacaacga tcctatatgc acttcctttc attcaaaaga aaaatctatt tgtaattaca
      301 aacagtttga ttgctttcaa ctatttaaag aatcatacaa gttatcggat tttacttact
      361 ggtggcgaat tttctcctat tacagaagag ttcattgggg aagttgcaga aagatctttt
      421 gaaagtttga atgtcgatat ctctttcgct gcaacaaacg gcatatttga taacaatgtg
      481 acaaccgctc aatttattga gggaaatatt cagaacgcag cgctcaatgc agcaaaaatc
      541 aaatgcattg ttgcagatta ttcaaaatta aataaatcag acgtctatac ttttagaaaa
      601 ttaaccgatt ttgattatct aataaccgat aatcatttat cagaacagga tttcaatcat
      661 tattcaaaat acttaacaat attaaaggag cccaaaaatg atactgacaa tcacgatgaa
      721 cccatccatt gattccattt attttacaaa acactttgaa ttaggtacga tgacacgctt
      781 tgattctccg gtacgctatg taggcggcaa gggaatcaat tgcggacgag cttctgctct
      841 actaggttca tcagttattt taacagggtt tttgggcggt tattttggca gtatgattca
      901 agatatgctg aaaaatgaaa atttgtttga tttgcatttt ttacctattg cagaagaatc
      961 aagaggtgcc gtcacgatca tgcatgatga tgaaacgcag acagagctag tggaagctgg
     1021 tcctgaaata gcagatacta ctatcaaaaa attaatgaat gatatcaaac taatcagtaa
     1081 aaataattac ctttcagtca ttacaataaa tggttctgtt aacaatccag atcccttttt
     1141 gtatgctaaa ttattacaat atatcaaaga acaaatagat ccttccacct atatattaat
     1201 ggatatatct ggacaacatc tccgtgaagt cttagcaagt aagtataaac cgatgttcat
     1261 caaacctaat atccatgagt tttcagatat catccatcaa aaaattactc aaaaaagcga
     1321 cataaaacac catctaaaaa caaatccact atttaaagat atcgatgtta tcatgatttc
     1381 ttgcggctcg tatggtgcag tagtcaaagt aaaagaagaa ttttggaatg ttgagatccc
     1441 taagattcct atcattaata caacaggctc tggggatgcc actgttggag gttttgctca
     1501 ttcattagaa cagaattatc ctttaaaaga atgtgtaagg tacgcaatgg cttgcggcat
     1561 gtcgaatgca cagcaatcag aagtcgggaa agttattcca gctgatgtaa aaaatttagc
     1621 aaaaagcatt aaaatcacag aaatgaaata aatagtacaa agtaaataat tgtttatatg
     1681 aaatgagaca caaagagact gtgacaaaaa acgtgtgtac cgacccccaa aagttagatt
     1741 ttttggtcta acttttggga gtcgatacat tgtcacagtc cttttttcga ataaacggtg
     1801 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctatttt
     1861 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacgacctct tggtataaac
     1921 ggtgttcaaa agcttgcatc tacgctaatg agtccaacca agtcaaaaat gaggagcttt
     1981 tttggctgtc cttattactt gatgcagggc agttttttca taatctcttt tagattaaat
     2041 ccttttggtt acacaaggtt ctcttcaaag aatgttgcca ttttttcaaa tggaatgatg
     2101 tctgtctggt cgtatagatc tgtatgggat gctcctggaa tgatcatgag ttccttgttg
     2161 tcacctttta gttctttgaa agcgtcctcc ccaaagtaac gtgaatgagc tttttccccg
     2221 tgaatgacta agactgcatg acggatttct ggaatataat gcaatagttt tgcattcata
     2281 agtgacaacc ctgatgtcct attccaacca tcattggagt ttaaagaacg gacatgatat
     2341 ccgcggtctg ttttataata cgcatgatag tctttgacaa attgaggagc atcttcaggc
     2401 aacggatcaa ccacacctcc agcacgtgcg tagtcccctt gtttgaaatc aatgatgcgt
     2461 tgtgcattta attcttcacg catttcatat cgggcatttt catccataac atcgaagtag
     2521 ccatttgctg ttactcgagt catgtcatac attgtggaaa caactgttgc tttgacccgc
     2581 gtatcgatag ctgctgcatt caatgccata cctccccatc cacaaatacc aatgattccg
     2641 attctctcag ggtttacatt tttttgtaca gataaaaagt ctactgccgc ttggaaatct
     2701 tccgtattga tatcaggcga agcaacatat cgaggtactc ctccgctttc tcctgtaaac
     2761 gatggatcaa acgcaatcgt taaaaagcct ctttcagcca tcgtctgcgc atatagacca
     2821 gacgattgtt ccttcactgc accaaatggc ccgctcacag cgattgccgg caatttcttt
     2881 tcacttactt cttttggttc atataaatca gcgactaatg tgataccaaa acgattcggg
     2941 aagctcacct ttcgatgatt gacttgattg ctttttggaa atactttgtc ccattcttgt
     3001 gtgatgttta attctttatt catattctag actctccttt gatcaaaatt gacatttgtt
     3061 tttgaatgta ttattatgat aaaacttaaa cttgacttta ggtcaaggta aaaattttat
     3121 tttaggagga tttttttatg tattccattg gacaagtgtc tgaaatgttt ggattgcctg
     3181 tttctacttt acgttattac gacaaagagg gattatttcc tgatatccag cgtgtttctg
     3241 gtattagaaa attttcagag aatgagttag aggctttaag agtcattgac tgtttgaaaa
     3301 aatctggttt agaaataaaa gacattaagc aattcatgga atggagcaaa gaaggcagta
     3361 agacatttga ggttcgtaaa gaactatttg aaaaacaaaa agaagtagta gaaaaagaaa
     3421 tagcaaaatt agaaagagta ctcgctatgc taaactacaa aagctggtac tatgaagaag
     3481 caataaaagc cggcaatgaa gaagccgtat tgacaatgat tcctgatgat ttaccccaac
     3541 atgtaaaaga agcttatgta cattctcatt gaacaaacgg ataactcact ctccttctct
     3601 atgaacttca tgaaaacatg aataaacacc tctaactgat tgttagaagt gtttattttt
     3661 tcactcaagt atcctgcact tacaaaaata tcctttttac cgccatccta attctggagc
     3721 gatatctttt acgattgttt caagcaagtg catgttatat tctaccccca aagtattagg
     3781 aatcgtgaca agtactgtgt ctgcttcttt gattgcttca tcttcagcta gctccttgat
     3841 taattgatca ggttcaccag caaaagtttt tccaaaaatc gttgggtttc ggtgataagc
     3901 aagatagccg acagcatctt gttgcggact accgtcttgt ccaaataagc gtcgatccaa
     3961 atcagtggtg attggctgaa tcgagcgagt aactaatgtt cttggttcaa aatcatgacc
     4021 tgcttgcttc cacgcttctt tatacgcacg taattggttt gcttgttgga tatggaatgg
     4081 ctcgtgggat tcgtaattga ctaatgtaga tgattgcaga ttcattccat gttgtgctgc
     4141 ccagattgcc gtttgctgcg aaccagctcc ccaccagatt ctttgtcgta atccttctga
     4201 ataaggttct atccgtaatt ttcctgatcc ttgtgggaac attggttgag gatttggttc
     4261 ttcaaatggt tttccttcaa gtaattccaa gaattcaagg gtgcgttccc gtgttacctc
     4321 tttgatttct tcttcactgt aatcataacc aaagtatttc cagccatcta gaacttgttc
     4381 aggtgaacca cgccccactc ctagttgcac tcttccttgc gtgatgatat ctgtcaatcc
     4441 ggcattttct gccatataat aagggttttc atatcgcata tcaataagtc ctgtaccgat
     4501 ttcaatctta cttgtctttg cgccgattgc cgctagcaaa ggaaacggcg acccaatctg
     4561 aggggcaaaa tgatgtacgc ggaaaaacgc tccgtccact ccgatttttt ctgcttccac
     4621 cgctaaatca atcgattgca aataagcatc ttgagctgat ttcacaagcg atccttgctc
     4681 catccaatgt ccaaagctta aaaagccgat ttttttcaag gtaatgacct cctttaaacc
     4741 tacaaaaatg gaaggttttt tatttttaat atgaactgat tctactactt tttttcttaa
     4801 aagacgaata attgttctcg cctcacaatc aagaaatgta tcggcttact acacccctac
     4861 tttcttcatc atctgccgtg tcttaatctc ttttaacagt ctgttacatc gttgattttt
     4921 gactcaaaat gattgtttct aacatgtatt tatggtaaaa aagacaagat tagttctttg
     4981 tacacatcga tttatacgct gaacaaaaag aaatatataa actaagaaac ccattgtttt
     5041 ttcgctccat cacatagagt ttactttaga taaacagtga catttactaa tccataacgg
     5101 aggaaatcaa atgaaaaaaa tctatttctt atgtacgggc aattcatgcc gcagtcaaat
     5161 agcagaaggc tacgcgcgta aatacttgtc tccttcaatg tttgagataa gaagtgcggg
     5221 gatcgagaca catggcttga atccaagagc t
//
LOCUS       DAYYHS010000135         5067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000135 DAYYHS010000000
VERSION     DAYYHS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.138"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..354
                     /locus_tag="ACHA6A_002808"
     CDS             55..354
                     /locus_tag="ACHA6A_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970923.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            619..1494
                     /locus_tag="ACHA6A_002809"
     CDS             619..1494
                     /locus_tag="ACHA6A_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970924.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1491..1709
                     /locus_tag="ACHA6A_002810"
     CDS             1491..1709
                     /locus_tag="ACHA6A_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970925.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1696..4323
                     /locus_tag="ACHA6A_002811"
     CDS             1696..4323
                     /locus_tag="ACHA6A_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8970926.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4338..4556
                     /locus_tag="ACHA6A_002812"
     CDS             4338..4556
                     /locus_tag="ACHA6A_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970927.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         2003 a          628 c         1004 g         1432 t
ORIGIN      
        1 tacttataaa ggtagaggag ttatagaata attaattaaa aaggagaggt ttttgtggaa
       61 gcaataaaat taatgatttc agaagaacag gaggaaatgt ttctaaagca gttttatcaa
      121 attgctacaa aagctattga aaaggctcaa gaaaatgcgg ggatgacaag ggaatatctc
      181 aatcaaaaag aaatggcgga atacttaaat gtgagtgaaa actttttaaa aaatgctgtg
      241 agaaatgagg gattgcccgt agttgaatta ggggcaagaa aattttattc gaaaaaggca
      301 gttaatgagt ttatgtttgc tcgaatgaaa agaggggaaa atattggaag atgatgaagt
      361 agaaattata aaaaaatgct ttaaaaaaag aaaaaaagtg ttcccaaaag tttgcagact
      421 gggggaacac tcaataataa agtgctgaga aaactttatt tatagctatt atatcaaaaa
      481 agaaggtgaa aaggtgtttt cttctggaaa tgttgtagtt gacaaactta gagaaataaa
      541 ttttgacgga aatgtaatcc acacacatgg tacgatcaat taagaaaaaa aacgaaaaaa
      601 ggtgttgaga agccctattt gaatgcaata gttatcttag cagaaattac ctattggtat
      661 agacctaaaa aaatttttaa cgatgatggt cagctacttg gttatcaaaa aaagtttctt
      721 gaagatattt tgcaaaaatc atataagcaa ttaagcaaaa aaacaggatt atcagaacga
      781 gctataaaag atgcaattgt gtacttagag aaaaagggga ttatcaaaag ggtttttaga
      841 aatatttatt atgagaaaag agcagttaca ttatcaaatg ttatgtacat tgttttaaat
      901 gtacataaat tgatagaaat tacttttcct actttggatt gcgaaaaaaa ggagtatatg
      961 ccgaaaattc gtcagagggg tatcgaaaaa aagagatatg tctctttaaa aaaaggtaga
     1021 ggtatgtaca aatttgtata tacgtataca aagactactt cacagaacat atcaaagact
     1081 actctcgtta tggaagaaga ggatattaat ataaagagga tttacagtaa gattagtgtt
     1141 ctttatgacg caaataacga agatggtcga aaattatttc aagtgtatca ctatttgaga
     1201 aacgctaatg tagatacaga agatatcaga aaaatcattg aatatttatc tgaaaatact
     1261 cagtatctta ttgctgagtg tattataaaa caacatgaag catgcttttt tcaaagaaaa
     1321 actgaagaag ggttatacga ttatgtaaca tattttttga atggacttca gaaaagatat
     1381 aaaagtcaat ttttagaaaa tgtagagcct atagatcagt tcaaaaaagc tttcgaaatc
     1441 aaagagttac ctgaaattac tatgcataat tggttgaaag gagaagaatc atgattaaaa
     1501 atcgcttaat agatcaaatg tggtgtcttg atcaaaatac agatactctc gaattaatcg
     1561 agagtatctg cttcaacaca gtagttctcg atttaagagc tgaaaatgac aattgtgtaa
     1621 ctcatatcat aattaatcaa aaaatggctc agcagctctc tgagagccta agaaaatggg
     1681 ctgaaggaga aaactatgaa agcaattaaa gaaaaaatcg ttagtagaaa aataaactgg
     1741 ttagcactca gtgtacttgt tttaatgatt agcgttgttt ttacaagtaa tattgttgaa
     1801 gctagcattt tcaattggtt tgatgacaca aaaagtcaaa cagagttttt gaataattct
     1861 ctttatagac cttacctaaa aaaatacaat ggagatttcc ttagtcagtt cgggatttgg
     1921 cttggatggg ctgtggtaaa aggcatgttt actgtgactg attcgattca aaatatgatt
     1981 ccagatgtgt tggatttgtt caatttcatt gaaagtacag ggctaaataa tgtttatcaa
     2041 tcagtcataa atacaattgt cgttggatta atgatccttt cattgatgtt tgttggctac
     2101 aagatgatta ctggaaaagg aacaatagat ttaagatctg ttggaatgaa tatagtgatg
     2161 tctgtagcgt taattctttt aatgccaaca atgatttcta gcggtattca atttgccaaa
     2221 atattttaca atgatgctac tacgattaca aattcagatg acggagtcgc ttggtcattg
     2281 ataaaacaag gagtaacaga tctggcatat attaataaaa cagatcaata ctcaagcatt
     2341 gataaaacgg aggatcgaaa taagttaaca cgaaaaaatt ttcaacagac agatttaaca
     2401 caagtgttga cggataaagt gattgataaa ttggaaaaag aaaatccagc tgcggataat
     2461 cttcgctatg aattagttga aaattctaat aatgaatttg ttgccacaaa gttttctgat
     2521 aacttcttat caacattttc tgatagcttg aaatcaggtt attatcgata tcaagcaaat
     2581 ctttggggga tttcgattgg gttgacagcg ttggcaatcg cctatgtgtt cagcgcgttt
     2641 gtgatcatta cagcaatatt agaacttgca tttaaacgtg tgttaggtgt tcttgttttt
     2701 gcgacagata ttgaaacagg acaacgttca aaagttgttt tatccgatat tttacaatgt
     2761 tacttaacag ttggatttca agggtttgga ctatctatgt ttgctatgtt tatcaatttt
     2821 ttaaatgctg gacaaggtat ctccacaaat atttttataa aaacaattgc ttatatctgt
     2881 gctgtgtttg tgctgataaa aggcagtgga acagtcatga gatactttgg tgttgatatt
     2941 ggactcaaag aaggctatgg gcaacttgct tctgcttttg gtatgggcgc aatgctgttt
     3001 agaaaaggat caactggatt taatcgtgct aaaggaagcg gaaatggaaa tggatcagac
     3061 agtggagaag gagaagatcg aaaaccagaa aagaatttcg gcgaaactct atcaaaaaaa
     3121 gctggcaaaa ctggaagagc tttgggatat gcccatgaac gtggattatc aggtttagct
     3181 tcagatggag ctacaatggc tagtgaaaga gcaataaaac catttaagag tatgcgtgat
     3241 atggctaatg ataccaagaa taagttcaaa gaaggtttag atgatggtac agtgtccgct
     3301 attaataaaa atagcaaacc tatgttagct aaaaataaag aagatgaaac tggaaaatat
     3361 gctgattcca tgccaacacg tctttcggat agaaaagatg gtgctaaggt tgaaaatgcc
     3421 gatagaatta tgtctagttc tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg
     3481 aataataatc ctgtaagtgc aattcagcaa aaagttcaac aagatattga agaacgtaaa
     3541 aatgctatgc acggtcaagc taagtctgct gaagaattaa ttaatcagaa gcgacaagaa
     3601 gctaaataca cgcctgaagc aatgaatcgt gaggaaatgt taaggaaacg tgtagaaggg
     3661 cgtacaggag ctaatacgga cgaaaaagaa gcgttgacca aagaaagaat ccaagaagct
     3721 aaaaactcaa ataccggatt agaaaaattg gtcaaagaaa atctacaaaa ttctacaagt
     3781 agtcaaggtg gccgatcagt agatgtccga gaaaatattc aaggttcatt gaatggtcat
     3841 gtcggacgga atgttgatgt aagagagaat ttacaaagct cttcaaatgg tcaaggtact
     3901 aagacaattg atgttcgaga aaatgtacaa aattctacaa gtagtcaagg gtttaaatca
     3961 atggatgtaa gagagaatgt acaaaattct acaagtagtc aagggtttaa atcaatggat
     4021 gtaagagaga atgtacaaag ttctacaagt agtcaaggat tcaaaacagt agatgttcgg
     4081 gagaacgttc aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat caatatcgtt
     4141 gaagacagaa agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat tattgatagt
     4201 gaaatccgtg aaaatgataa gggatcaaaa ccaagacgaa gatttaccta tgaagacaac
     4261 gagttattca gagatacact taatgatcct aatccgcttt tcgataatct attaaaaaaa
     4321 taaaaggggg aattgtcatg aaattaattc taaagattag tacgcatgat ttcttagaac
     4381 ttgacgaatt atgtgaatca gtaaagaacg caacaaatga aaaaatcacg gtcactaatt
     4441 atattgaact gatgattcaa gaagaattaa agatgaaaca tgagataaaa aacaacttga
     4501 aatattgtta tctagaaaaa agtgaagaga taatccatga attaaatggc ttttaaaaat
     4561 tgtatttgac atgaatttac gacaaggaat tagtggggat taagcgatta ttctcttcca
     4621 ctttagagaa ataatgggtt aaaaagccag aatacgtaga gcaatattga caacctataa
     4681 aggagctatt gttgaaaatt gaagtaacaa aaagataaag ggaggtttac atctatcaga
     4741 ttaaaagtga tagtacaccc atgaaacctg caaaagagtt gaacctaatg tatatcggtt
     4801 taactctttt tatttgcaaa ttaaaaaaat atcaccgaag tgacgaatga aactatgacc
     4861 aaattaagca agaaaatata tcttgcaaat aaagtagtat aaaaagatat tgataaagcc
     4921 aatcaaaact ttgaatttca aacgttagat aatcaaacag atacaaaaaa tgaaatcagt
     4981 tctacgcgta cgattattaa aaataataaa ttttcactag aagaaaatgg aaataaaatc
     5041 aaataattag gaagaaaaaa caaagca
//
LOCUS       DAYYHS010000136         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000136 DAYYHS010000000
VERSION     DAYYHS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.139"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHA6A_002813"
     CDS             85..972
                     /locus_tag="ACHA6A_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8970928.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHA6A_002814"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHA6A_002814"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8970929.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHA6A_002815"
     CDS             2274..2720
                     /locus_tag="ACHA6A_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8970930.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHA6A_002816"
     CDS             2993..4345
                     /locus_tag="ACHA6A_002816"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8970931.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHA6A_002817"
     CDS             4516..4803
                     /locus_tag="ACHA6A_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8970932.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYHS010000137         4831 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000137 DAYYHS010000000
VERSION     DAYYHS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.140"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA6A_002818"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA6A_002818"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8970933.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHA6A_002819"
     CDS             complement(1297..2133)
                     /locus_tag="ACHA6A_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970934.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHA6A_002820"
     CDS             complement(2317..3534)
                     /locus_tag="ACHA6A_002820"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8970935.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHA6A_002821"
     CDS             complement(3683..3844)
                     /locus_tag="ACHA6A_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970936.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHA6A_002822"
     tRNA            3917..4000
                     /locus_tag="ACHA6A_002822"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..>4831)
                     /locus_tag="ACHA6A_002823"
     CDS             complement(4240..>4831)
                     /locus_tag="ACHA6A_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970937.1"
                     /translation="VFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIY
                     FGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCL
                     IVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLM
                     AVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1474 a         1005 c          777 g         1575 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac g
//
LOCUS       DAYYHS010000138         4620 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000138 DAYYHS010000000
VERSION     DAYYHS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.142"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..959
                     /locus_tag="ACHA6A_002824"
     CDS             153..959
                     /locus_tag="ACHA6A_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8970938.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            977..1684
                     /locus_tag="ACHA6A_002825"
     CDS             977..1684
                     /locus_tag="ACHA6A_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8970939.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1681..2652
                     /locus_tag="ACHA6A_002826"
     CDS             1681..2652
                     /locus_tag="ACHA6A_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8970940.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2645..3673
                     /gene="vanB"
                     /locus_tag="ACHA6A_002827"
     CDS             2645..3673
                     /gene="vanB"
                     /locus_tag="ACHA6A_002827"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8970941.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3679..4287
                     /locus_tag="ACHA6A_002828"
     CDS             3679..4287
                     /locus_tag="ACHA6A_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8970942.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4353..4526)
                     /locus_tag="ACHA6A_002829"
     CDS             complement(4353..4526)
                     /locus_tag="ACHA6A_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970943.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1300 a          972 c         1230 g         1118 t
ORIGIN      
        1 attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga aaactacaga
       61 ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca gccacaaatc
      121 gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat cattccaatg
      181 cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt ctatgtgcgt
      241 tcattatttc gttcacagtc tgcacgctgt ttttggggtg gagactggct tccgtattgg
      301 aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact gacgtagtgg
      361 agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa caggaatgga
      421 gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg gagcttgagc
      481 aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa gatttgtttg
      541 atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga acaacagaaa
      601 aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc tacacctctg
      661 cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc gagcatcagc
      721 ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac gaggtttata
      781 gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca ccagacaaga
      841 cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc atcgaagctg
      901 ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca gaaaaatgag
      961 aaaaggatgt tatgttatgg acagaaaaag attgacacag cgtttcccgt tcctgcttcc
     1021 aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg acggctgtcg
     1081 ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
     1141 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
     1201 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
     1261 ctggttgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
     1321 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt cagagggctg
     1381 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
     1441 tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag ccccaagcat tatacaaaat
     1501 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
     1561 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
     1621 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
     1681 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
     1741 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
     1801 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taagccataa gtccgaggtt
     1861 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
     1921 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
     1981 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
     2041 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
     2101 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
     2161 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
     2221 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
     2281 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
     2341 atgaagcaag gcgcattttt aatcaacacc gggcgcggag cgcttgtcga taccggggcg
     2401 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
     2461 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
     2521 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
     2581 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
     2641 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
     2701 gtcggtaaaa gccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
     2761 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
     2821 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
     2881 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
     2941 caaatgcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
     3001 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
     3061 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt gataaaggtg acaagccgga
     3121 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
     3181 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
     3241 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
     3301 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
     3361 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
     3421 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
     3481 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
     3541 catcgttcta aatgaggtca ataccctgcc cggttttaca tcgtacagcc gctacccacg
     3601 tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
     3661 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
     3721 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
     3781 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
     3841 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
     3901 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
     3961 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
     4021 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
     4081 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
     4141 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
     4201 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
     4261 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
     4321 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
     4381 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
     4441 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
     4501 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
     4561 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaaagc cttgcccctc
//
LOCUS       DAYYHS010000139         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000139 DAYYHS010000000
VERSION     DAYYHS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.143"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHA6A_002830"
     CDS             186..2855
                     /locus_tag="ACHA6A_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8970944.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHA6A_002831"
     CDS             complement(2946..3560)
                     /locus_tag="ACHA6A_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8970945.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACHA6A_002832"
     CDS             3680..4219
                     /locus_tag="ACHA6A_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970946.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHA6A_002833"
     CDS             4216..4554
                     /locus_tag="ACHA6A_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8970947.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAYYHS010000140         4512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000140 DAYYHS010000000
VERSION     DAYYHS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.144"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..2021
                     /locus_tag="ACHA6A_002834"
     CDS             81..2021
                     /locus_tag="ACHA6A_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8970948.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2108..4030
                     /locus_tag="ACHA6A_002835"
     CDS             2108..4030
                     /locus_tag="ACHA6A_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8970949.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4310..>4512)
                     /locus_tag="ACHA6A_002836"
     CDS             complement(4310..>4512)
                     /locus_tag="ACHA6A_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFM8970950.1"
                     /translation="DLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSEEEILTE
                     FRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1493 a          766 c          933 g         1320 t
ORIGIN      
        1 tcagttatta cggaatcatt ttgtgcttaa taaaaagaaa ccgtttttat atactataaa
       61 tgtagaaagg cggtaggaaa atgaaaaaaa ttattgcaat cacatcctgt ccagttggta
      121 ttgcccatac atacatggca gcagaaaact tagagaaagc ggggaaagca gttggcgccg
      181 aggtaaaagt cgaaacacat ggttccatcg gtattgaaaa tgaattgact gcaagagata
      241 tcgaagaagc agctggtgta atcatcgcag cagatactaa aatagataaa tcacgattcg
      301 gcggtaaacc tttgataacc gtaggtgtgc aagaaggaat ccaccatgca gatgagttgg
      361 tgagagatat tttagcagga aaagcgccag tctataaaag tacggaggca attatttctt
      421 ccgaaaataa aagtgaatca gaaaatttag gtaaaaaaat ctataagagt ttgatgaatg
      481 gcgtttcgta catggtacct ttcgtagtta caggagggtt attgatcgcg atttctttaa
      541 cattaggagg aacagctaca cctgaaggga tcaagatacc agaaggaacg atttgggcaa
      601 cgatgaatag tatcggaagt atcgctatgg ggttgatggt tccaattcta tctgcattta
      661 ttgcgcaaag tattgcagat cgtccaggac ttgttcctgg atttgttggt gggatgttag
      721 cggcaaacgg agcattatat ggtagtgatg cgaatgcggg attcttaggc gggatcatca
      781 ctggattttt agcaggattc attgctttag gaatcaagaa agtgcgagta ccgaaagctt
      841 tacaatcgat catgccaatt attgttatcc caattttagg atcattggca gttggttttg
      901 tgtttatcta tgttattgga gaaccagtag ctcttctatt tagttcattg actcactttt
      961 tagcagggat gcaaggagga agtgaaattg ttctagctat gatcttagga gcgatgattg
     1021 cttttgatat gggggggccg ttcaataaag tagccttttt gttcggatct ggcttgattg
     1081 cagaaggcaa ttattccatt atggggccaa ttgctgtagc tatctgtatc cctccattag
     1141 cattggggct ggcatcattg attttaaaaa ccaaatttac gaaaacagaa cgagaagctg
     1201 gtaaggcgtc cttagcaatg ggactgtttg ggattacaga aggcgctatt ccgtttgctt
     1261 caacagatcc actacgggtg attccaagca ttgtaatcgg ggcaatgaca ggaagtgtga
     1321 tcgcgatgct ttcaggagtg acagatcatg tagctcatgg cggtccgatc gtagcggttt
     1381 taggagcagt ggatcacgta gccatgtttt ttgtagcagt gattgctggt attgctgtaa
     1441 ccgtgctaat gttaagaatc cttaaaccga cattacaaca aactgcggtt gctgcagcaa
     1501 atggtgtgac agtagcagat ctatcagaga ctgaaacaag tgatgtttat acgaaaactg
     1561 aaacttttga ttctatcacg gatattatgt cagatgaagc aatcgtttta ggtgaaacag
     1621 caacaacaaa agacggagta atcgaagatt tagtccaacg tttagaaaaa acagcggcga
     1681 tcacagataa aaccagattt aaagaagtca tctatgatag agaacatgaa agtacaacgg
     1741 gaatcggtat ggggattgct attccacatg gaaaatcagc ttatgttctt aaaccaacag
     1801 tggcatttgc cagaagtcaa tctggggtag attggcattc tttagatgga aaacctgcac
     1861 atatgatctt tatgattgct gtacctgaaa acagccaagg tgatatgcac ttgaaaatac
     1921 tgcaacgtct atcacgtaaa ttgatggatg atgacttccg tcaagcactg atggaagcgc
     1981 cagataaaac aacagttcac caattattaa gcgaaatgta acataagatg aaaaatcgac
     2041 cgtgatgaga cataatagat gtgttatcat gatcgatttt ttatttagaa gggagtttct
     2101 gcacattatg aatcagcgtc agcaatcttt gatctatcaa ttaagtgaga acaataatta
     2161 tataacaaca aaaaagctag cagagcattt ccatgtgtca gaaagaacga ttcatagcga
     2221 tttgaatacc attgaaactt ggttgagaga caaacatctg cctttaatgt ttgaaagaaa
     2281 aaaaggtaca ggtatcttgt tgaatggtct gcctcacgaa agggaacaac tgaaacgagc
     2341 gcttaatgaa gagactcaaa cagaaatgga taaaaaacag cttcgtcagt tattgatttt
     2401 ccatctgcta gtagcaaaag acggatttag tcttgaagaa ctttcagaaa aactatatgt
     2461 tggaaaacgt acgattcgta aagaattgac agagttgcaa agcttttttg agtatcacca
     2521 tttacagcta gtatccaaaa caaaactagg aacttttctt aaaggcgatg aacaagaaaa
     2581 aagacagttg cttgttaaga cattacgaaa tatgcagaaa gaagatccac aaagtccgtc
     2641 gttgaaagaa ttcttcgcta aagatacatt aaaagttatc caacatacat tacaagatgt
     2701 tttttatgaa aaccatttag aacagccgaa tggactggct agtgtggaga ttcacatcta
     2761 ctttatgctc gagcgaatga aacagtacca gaaagtgaaa ttaagtaaag acgaaaacga
     2821 agtcgtggaa cacacccaag cacaaaagtt aagtagtcaa atattagcaa aactagccac
     2881 catctatcca attgaatttt ctcctgatga aatcaattat ctggctttgc gtatcgccaa
     2941 tctttttaca aattcacaag cagccacacg ttttcaaaag gaatctagcg cacttgctga
     3001 tcatttgatc gaacaagtgg aacagtttct aggctattca ttaaaagaag atcagctact
     3061 aaaacaaaat ttaaggtctc atttatcttc aacttatttt cgactccatt atggtttgca
     3121 gatttctaat ccattgacga aaaatgtgtt tagtacgtac acacaacttt tcttagtttt
     3181 acagttaatt ttagaagatt attttgaaaa ggaaaatttt tatgttccac aagaggagac
     3241 agcttattta acgatccatt tccaagcagc gatcgagcga cacaaacatc agaaaagtag
     3301 aagatatcaa acggttttag ttagtgaata ttcaaaagct atggctactt ttctcgaagc
     3361 acggctgaac agagaacttc cagaattaac gattatggat ctagttgaat ataagccaga
     3421 attaaagaga caagattttc cctctgttga ttttatcttg gctacgttac catttaaaca
     3481 tataagcatt ccagtaatcg agatctcacc aatgatcaca gaaactgatc tagcctattt
     3541 gacaaaatat atgctggagc atgtgcctat caagaagaaa aaaacatttg atctagcaag
     3601 ctttactcat ccatttctga tctttcctca acttgaacgg acagacccag ccgatatttt
     3661 aaactttatg ggaaacgtct tagtggaaca tcattatgtg gagccggaat tcatcgattc
     3721 tgtcttggaa agagacagtc actccagtac tagagtagca ccatttgtaa caatccctca
     3781 cggtaatccg ctgtatgtga agcattcgat gatttctatt gctacaatga aagaaccagt
     3841 cctatggcac ggggaacaga tacgtatcgt actgctatta gcaataaaga aaaatgattt
     3901 gaaacatgca gagttcaaaa aaatattttc tgttattcac tatctagaaa agcagccaga
     3961 acaaatggct cagttgatgc aatccaatca tccgctagaa atcttaacgt tgttatctag
     4021 ttatgaataa gtagcgatta aaaaactgcc tttatattta taataattct atctatttat
     4081 ttggaagtag aaaactaaat acaaaaaaag aggatgtggc caaagttgtg gcacatcctt
     4141 ttattttgag tttcttataa ttttttatag ttgcttacga tgtcttgact actggtaaac
     4201 ggtgttcaag agacaatttg tcggcaataa ggcacaattt tgtaaaatat ggaaagctat
     4261 ttttttaaca ttgtttctta gtgcttgaaa aatcattttg aatacttctt tatacgatcg
     4321 atgtacgttg atttaagtct aaaacttttc ttttgattcc ttctctgact tttctgaatt
     4381 ccgttaatat ttcttcttcg ctcccgacag cttgtgcagg atcttttagg tcccagtgaa
     4441 ggtgttcgtg attgtttgga acggcagggc acttgtcttt ggcatcacca caaagtgtaa
     4501 tgatcaagtc tg
//
LOCUS       DAYYHS010000141         4466 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000141 DAYYHS010000000
VERSION     DAYYHS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.145"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..851)
                     /locus_tag="ACHA6A_002837"
     CDS             complement(<1..851)
                     /locus_tag="ACHA6A_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8970951.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQP"
     gene            complement(853..2292)
                     /locus_tag="ACHA6A_002838"
     CDS             complement(853..2292)
                     /locus_tag="ACHA6A_002838"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8970952.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2477..4423
                     /locus_tag="ACHA6A_002839"
     CDS             2477..4423
                     /locus_tag="ACHA6A_002839"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8970953.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1407 a          770 c          749 g         1540 t
ORIGIN      
        1 attggttgtc ttattgttgt caattgtggg aaaaatttct caataaaaga ggtaccatcg
       61 tatccaataa tttttaaatc ttctgggata ttaagtttaa gttgatgagc aattttcata
      121 gtgagaatgg ctgttaaatc atcagaaaca aaaataccat ctggtttgtg atttataata
      181 gttgacttta tttccatttc acgacggata gtagacagat tattaggaat tttgaaaatt
      241 tttccatttg gattttgaaa agaaaaacct agtgcacgta attctgtggg agaatccgtg
      301 ttatcatttc ctgtaatcat aatggtattt tgacaaccat ttttttgtaa agtcttagct
      361 gcaagttttc ccccttcaaa gttatctgaa gaaacaatgg aaacattagg agctaaattt
      421 ctatcaaaag caacaatagg tgctctcaca cgctcataat cagcaattcc aagattatgg
      481 cttgatgaga ttatgccatc aacttggttg gcttcaagca tttcaatgta ttctctctct
      541 ttagcaggat cattttgact attgcaaata atcgttttat atccatgttt aaataactct
      601 atttcgagat actcaatgag ttcagagtaa aagatattac taatattagg aaaaataaga
      661 ccaataagct gaggtgactt tccttgaagt ccacgtgcta aattatttgg tttataacca
      721 agagttttca tagcttcatg aactttttgt tttgtttttt ctgataaata tcccttatta
      781 ttaatgacgc gtgaaacggt tgttgggcta actccagcta attcagcgac atcagttaat
      841 tttgctacca ttttaatatt ttaattcaaa gtattttcca gtaggttcgc ctgatttgat
      901 ttgaatacca ttttggtgat tttcagggaa tacacgacta gtaaacactt tttctccttt
      961 attaataaaa atctcaatga ttgagttatc tacaaaaatg ttagcagttg tttctttagt
     1021 atcaattgag cattcacgaa tcgtaccaaa gtcagtggca tattgaatgc ctgctttgct
     1081 gcgatctaaa ataatttttc cttctttggt atctacaatc aagctaagtc catttccttt
     1141 actatcagag aaaagaagta actccgattt ctgatttgga ggaaaagtta gttcaagctc
     1201 ataagtatta tttgtttctg cttttgctgt aaattgttca gaactagcac gtaatgatgt
     1261 aatagcttct actggatatt ggtaaagttt accatctttt attgaaagtt cttttacaag
     1321 actcattgct ccctgataat catacttatc agttggataa tcgatatcag gtagtccaat
     1381 ccaactgaca atcagagcac gaccatcagg agtattgaat ccttgagtcg catacgcttc
     1441 aaatccataa tcaagatttt gaatttctga agcgcccaca agttttactt tttctatgtc
     1501 aagcgattga cagatcttaa aagtattcgg atagatattc ccataattta gctcagattt
     1561 atccaaccct tgaggacaat aaatgagaac aggttttttg tctacaaata ctagatttgg
     1621 acactcaatc atgtattctg aaccagtgcc gccaaaatca aggttgccaa cttcctccca
     1681 tgtttccacg ttattgtcat ccgctttgta aagtttgata aagcctgatt tttctaaatt
     1741 ttgtgctcca acaatcgcat acaattgtcc ttcataatta aacatttgtg gatcacgaaa
     1801 atgatcagtt acatcagcag gtttatgaat aagaacattc tcacctttcg taatgttgta
     1861 gtctttatcc atccatgctc cgatttgaag tgtatcacgt tcccaatttt catttcgaac
     1921 gtttcctgta tagagtaaga atagattccc ctctatttca tatgcagaac cggaataagc
     1981 gccgtggcta tctcgttttg tgtctggaag aagttctgct cctgttacat gaaaatgaac
     2041 taaatcatct gattcattgt gtacccattc tttcaaccca tgagcagctc caaatggcca
     2101 attttgataa aaaagcgtat attttccgtt aaaaaaagaa aaaccattag gatcgtttaa
     2161 caatcctgtt ttaggttcga tatgatagct agcacgccaa ggtgatcgtt ttacattatc
     2221 cttaatttta tccatctcat ctttagtcca atcagaataa gcacgatatc gtatttctcg
     2281 aggtaagttc atattttact ccttcaattt atctttttac aactagtatg gtaaacgttt
     2341 tccgttgaaa aatcaatcat ttcatttaaa aaaataaact atatgacaaa cgtttgacat
     2401 atttttaaga tatgctaaaa taaatttgca tttcagtgaa ccgttttcac gtaaaaataa
     2461 aaaaggagtg attcgaatga attacagtaa agtagcttcc gacgtaatac aagcggtcgg
     2521 aaaagataac cttattgcag ctgcacactg cgctacacgt cttcgtctcg tactaaaaga
     2581 tgacacgaaa gtgaatcaaa aagctttaga cgaaaatcca gatgtgaaag gcacatttaa
     2641 aattgatgga caatatcaag tgattatcgg tgcaggtgat gttaatttcg tttatgacga
     2701 acttatcaaa aaaactggac tttcggaaat ttcaacagat gatttaaaac aaattgctag
     2761 taaagataaa aaattcaacc ccattatggc attaattaaa ttattatcag atatttttgt
     2821 tccaattata cctgcccttg ttgcaggtgg tttattaatg gcattacaaa actttttaac
     2881 agctgctgga ctttttggac caaaatcaat tgaagaaatg tatcctgcga ttgaaggtat
     2941 ttcctcaatg atccaactga tgtccgctgc accattcatg ttcttaccaa ttttagtagg
     3001 tatttcagct gctaaacgtt ttggagcaaa ccaatttctt ggtgctgcaa ttggtatgat
     3061 catgacaaca ccagatcttg gtggctcaac tgaatattgg aatatttttg gttatcatgt
     3121 ttcacaaaca aactatgctt atcaagttat tccagtttta gcagctgttt ggatattatc
     3181 tatattagaa aaattctttc ataaaaaatt gccatcatct attgacttta cattcacgcc
     3241 attattatca gtaatgatta ctggattttt gactttcaca gttattggcc ctgtaatgtt
     3301 attgttgtct aatggaatta cagacattat tgtttggcta tataacacta ctggatttat
     3361 tggaatgggt atttttggag gaacatattc acttatagtc atgactggtt tacatcaatc
     3421 atttccagct attgaaactc aattactttc agcgtggaca aatggtatcg gacatggaga
     3481 ctttatcttt gtagttgcat ctatggcaaa cgttgctcaa ggtgctgcaa catttgccat
     3541 ttggttctta actaaaaatt ctaagacaaa aagtcttgct tcctctgcgg gtgtctcagc
     3601 acttcttgga attacagagc ctgccttgtt cggagttaac ttaaaatacc gctttccatt
     3661 cttttgtgct ttaattggtt caggaattgc tacagcagta gcaggattac ttaaagtagt
     3721 tgctgtttca cttggttcag caggtttctt gggatttctt tcaattaatg caacatctat
     3781 tccattttac gtattgtgtg aattgattag ctttgtagtt gcttttgcaa ttacttactt
     3841 ctacggtaaa acaaaagctg ctggtatctt taaagcagaa gaaattgctg aacaaacacc
     3901 agttaattct tcaaaagtag caacaacaga tccaatatct actgatgttg aatcaacaca
     3961 aacaaaagtt tcatcagaaa caattgttag tcccatttct ggtaaagtga ttagtctttc
     4021 ttcagtaaat gatccagtct tttcatcaga agctatggga aaagggattg ccattaaacc
     4081 aaatggaaac acggtccatt ctccagtaga tggtactatt caactcacgt ttgaaactgg
     4141 acatgcttat ggtattaaat caaataatgg tgctgaagtt ctaattcata ttggtattga
     4201 tactgtatca atgggaggaa aaggatttac acaaaaagtt actactaatc aaaaagttag
     4261 aaaaggcgat attcttggag catttgaccg ttcaatgatt gagaaagaag gtcttgatga
     4321 tacaacaatg attattgtga ccaatacttc ggattattca gaagtaaata tagtagctga
     4381 aaatacagtc actgagggta ctgatttact tgaaatcaaa taaatatatt tccctgagta
     4441 aagatggaat tttctttcca aaatca
//
LOCUS       DAYYHS010000142         4461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000142 DAYYHS010000000
VERSION     DAYYHS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.146"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACHA6A_002840"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACHA6A_002840"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM8970954.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACHA6A_002841"
     CDS             2298..3221
                     /locus_tag="ACHA6A_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8970955.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACHA6A_002842"
     CDS             3237..3776
                     /locus_tag="ACHA6A_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8970956.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAYYHS010000143         4335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000143 DAYYHS010000000
VERSION     DAYYHS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.147"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHA6A_002843"
     CDS             127..579
                     /locus_tag="ACHA6A_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970957.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHA6A_002844"
     CDS             593..1282
                     /locus_tag="ACHA6A_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970958.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHA6A_002845"
     CDS             1407..3758
                     /locus_tag="ACHA6A_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8970959.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHA6A_002846"
     CDS             3838..4233
                     /locus_tag="ACHA6A_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970960.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1394 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatt
//
LOCUS       DAYYHS010000144         4284 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000144 DAYYHS010000000
VERSION     DAYYHS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.148"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..338)
                     /locus_tag="ACHA6A_002847"
     CDS             complement(<1..338)
                     /locus_tag="ACHA6A_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970961.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
     gene            complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACHA6A_002848"
     CDS             complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACHA6A_002848"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8970962.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1495..2922)
                     /locus_tag="ACHA6A_002849"
     CDS             complement(1495..2922)
                     /locus_tag="ACHA6A_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8970963.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2953..4167)
                     /locus_tag="ACHA6A_002850"
     CDS             complement(2953..4167)
                     /locus_tag="ACHA6A_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8970964.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1349 a          914 c          764 g         1257 t
ORIGIN      
        1 ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa
       61 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
      121 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      181 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      241 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      301 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      361 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      421 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      481 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      541 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      601 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      661 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      721 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      781 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      841 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      901 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      961 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
     1021 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1081 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1141 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1201 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1261 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1321 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1381 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1441 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1501 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1561 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1621 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1681 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1741 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1801 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1861 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1921 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1981 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     2041 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2101 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2161 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2221 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2281 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2341 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2401 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2461 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2521 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2581 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2641 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2701 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2761 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2821 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2881 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2941 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     3001 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3061 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3121 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3181 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3241 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3301 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3361 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3421 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3481 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3541 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3601 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3661 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3721 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3781 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3841 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3901 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3961 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     4021 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4081 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4141 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4201 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4261 gacagacaat taataaaagc ttga
//
LOCUS       DAYYHS010000145         4253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000145 DAYYHS010000000
VERSION     DAYYHS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.149"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..758
                     /locus_tag="ACHA6A_002851"
     CDS             57..758
                     /locus_tag="ACHA6A_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8970965.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1330..2346)
                     /gene="tsaD"
                     /locus_tag="ACHA6A_002852"
     CDS             complement(1330..2346)
                     /gene="tsaD"
                     /locus_tag="ACHA6A_002852"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8970966.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2343..2807)
                     /gene="rimI"
                     /locus_tag="ACHA6A_002853"
     CDS             complement(2343..2807)
                     /gene="rimI"
                     /locus_tag="ACHA6A_002853"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8970967.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2814..3356)
                     /gene="rimI"
                     /locus_tag="ACHA6A_002854"
     CDS             complement(2814..3356)
                     /gene="rimI"
                     /locus_tag="ACHA6A_002854"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8970968.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3340..4062)
                     /gene="tsaB"
                     /locus_tag="ACHA6A_002855"
     CDS             complement(3340..4062)
                     /gene="tsaB"
                     /locus_tag="ACHA6A_002855"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8970969.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1255 a          912 c          735 g         1351 t
ORIGIN      
        1 aattctttaa aaaaagtgac aataaatata gtgaacaata aggagaggac tttttaatgc
       61 gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat tttgaacatt
      121 tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct tataaaaaag
      181 gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg accaaaggac
      241 tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac tatgtgaata
      301 caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact tatacagctc
      361 aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa aaattagcac
      421 gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta gttgaaatac
      481 atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta aaacaaaatt
      541 tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc accattcctt
      601 atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa actgctggac
      661 aagtaatcaa acaactacgt gattccggac tgttagatta tgacaaaaag cgttttactt
      721 tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa taagcagtga
      781 ccctgatatt tatcagggtc actgcttatt ttcctcccta aaacttctga aagatccggc
      841 agttttagat cactgggcaa gactcaaaga ttttctatgt gtgtttcagt tatgtaatat
      901 tcactgtttg caaaaaatac tgatagatta actagaggaa gcgtcttgtc ctcatccgac
      961 ctagattatt cgatattcct cgacaataaa cgattaatca gtgagggcaa tcagaggaac
     1021 ggataatgat gaataagctg taaaaaatag ggctttgaca aaagcgtgtc acagccctta
     1081 tttcgattaa acggtgtgta cctgtcaact cttcggtcac aagtcataat gttcataaac
     1141 atgaggagct gttttttgaa attgttcctt gtgaatcaga gttaaacgac aggcttacga
     1201 actcttgatt aaacggcgtt caaaagcttg catacgcggt aataagaaca gccaggtcaa
     1261 aaacatgaga agccgttttt cctgggctgt tcttattact tgatgcaggg cagatttttc
     1321 acaacctctt tattcagata aaaccaagct tggatcagca ttcaactgat agctagcaaa
     1381 atttcttttt tgcatttcca catgtgctgc tgctccaatc attgcagcat tatctccaca
     1441 aagtcgaaga gggggaatca ctagttctac ctcagggagt ttagttgcca aagcatcctg
     1501 caatccttca cgcaaccctt gattggccgc tacacctcca gctacaatca gttgcttgac
     1561 tggataattt tgacaagctc gaagtgtttt agtaatcaat acatcgatca cacttgcttg
     1621 aaagctagct gctaaatcat ttttgtctag atcttctcca cgctgttgtg cattatgcac
     1681 caaattgata aatgcacttt ttaatccgct aaaactgaaa tcataattat cttcatggat
     1741 catcgctcgt ggaaaatggt aattatcttt tccctgatga gctagctgat cgatttcttt
     1801 tccgcttggg taagaaagac ctaatactct tccaactttg tcataagctt ctcctgctgc
     1861 gtcatctctt gtctccccaa tgatttcata tgatccatct tcttgcatat agactaattc
     1921 tgtatgtcct ccgctaacaa gaagtgccat caatggaaac tggaatggtt taaccagacg
     1981 ggctgcatag atatgccctg ccatatggtt tacaggaata agcggcaatt ggtgtgccca
     2041 agcaaaagcc ttagctgcag aaataccaat cagcaacgag ccgacaagtc ctggtccata
     2101 agtgacagcg actgccgata aatcttctgc cgacacacca gcttctacca gtgcatcttc
     2161 caagcataag gtgatttgtt ctacgtgatg gcggctagct acttcaggaa ctacgccacc
     2221 aaaacgttta tgactgttta tttgagaagc aacgatgtta gataagatct ctgttccatt
     2281 tctaacgacc gctacactcg tttcatcaca acttgattca atcgccagaa tcaattcttc
     2341 attcatttca tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga
     2401 ttgcgataat atgatttccg tttacctaat atacgaaact tttctgattc ataaagtttt
     2461 cttgctgctt cattagatag acgtacttct aaaaaaacat gcagcgaatc ttttatttgt
     2521 tccgtaatca gtaattgtaa caaatgccgt gcatgccctt ttccttgttc tgaaactgca
     2581 acagcaatat ttgtgatctc gacttcatct attactttcg agtatccaat aaagccacag
     2641 atctggttat cttcaactaa aagcagataa tcagagtgag gctgttggat gtctgccaaa
     2701 aactgcattt tcgtccaagg tgaaccatgt ggataagcat tttcgctaat catccatacg
     2761 cattctgcca agtacgcttc atcaaaatct ttttttctgc aaatcaacta atctcactca
     2821 ttttctaaaa gttttatcat atccaacgca tcttcttttg tttcagtgta atatcttttt
     2881 ttgatcgttg ttgcttgaaa acctaacttc cgataaagtc tttgtgcatc ttgattactc
     2941 atacgcactt ctaatgacat cgtctcacag cgattcatta tagcaaagtt ctcgatttcg
     3001 tcaataagca gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg
     3061 atatggcaat ctaaaccgaa cgctcttgaa ccgataaaac caatcattct tccattatca
     3121 aagataccta gatatagatg aggaagcggt gacttcaatt ctgatataaa ggcacttttt
     3181 gtccatggca attcgccatt atagacttct cgttcgacat ccagcagctc tttgatatct
     3241 tctgtagtca attctcttag tacgtaacga cgatcgattt gttcgattgt tttttctgta
     3301 taaatcattt tatcttgaaa aaagcttctg gcatacgctc taaatttttt caacataact
     3361 ttcgtctcca ggtgtgtgcg tctccaacca tttttcttct gcttctaccc gttttaaata
     3421 gcgaggaaga aaagcttgtt tctctgcaac tggttcagct aaagcaccta attgagccac
     3481 agttacacca cttggaatat cccatgctgg aactgtatta atcatcgcac taggcaatat
     3541 ttcctttatt tcatagaaga atttgtgaca atcactcccg acgaaataaa tagcttcatt
     3601 cagcttttgt agttgtgtga acaaatcaga agcagaaata tgtacatctt caagtacaat
     3661 ttctaatcta ttttctttcc agcggtatgc cccagcgtat acattttttc ttcgagcatc
     3721 aaacatgggc acaataattt tatccacacc gatgcaatta gccgcgatgg ttttcagact
     3781 agatactcct actagctctt tatctaatgt ataagcaatc gtttttgctg tcgttacacc
     3841 tatacgaagc cctgtataag aaccgggacc atcagaaacc gcgattcggt caatgtcttt
     3901 tggacttatt tgaagatctg caaacaattg gtcgattgca ggcatcagtg ttacactgtg
     3961 gttgcgtttg atatttgttt gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat
     4021 cgccaaagtt tggtttgcag tatctattcc taatgtaatc atatatttga tcctttctgc
     4081 tatgacattc tcgccttcca ttgtagcata cttttttatt ctgaggatga aaataccaaa
     4141 ggcaaaacta gtacaatgat acatgattat ctgctggcgg gcaaattttg ccccttcctg
     4201 aaacacaaaa aagctgcggt atcacttcat ttccccacag cttttccatc ttt
//
LOCUS       DAYYHS010000146         4174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000146 DAYYHS010000000
VERSION     DAYYHS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.150"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            336..926
                     /locus_tag="ACHA6A_002856"
     CDS             336..926
                     /locus_tag="ACHA6A_002856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970970.1"
                     /translation="MNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLF
                     PHEDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEI
                     QYEGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMEN
                     NINYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            1042..1362
                     /locus_tag="ACHA6A_002857"
                     /pseudo
     CDS             1042..1362
                     /locus_tag="ACHA6A_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1540..1899
                     /locus_tag="ACHA6A_002858"
                     /pseudo
     CDS             1540..1899
                     /locus_tag="ACHA6A_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            1963..>4174
                     /locus_tag="ACHA6A_002859"
     CDS             1963..>4174
                     /locus_tag="ACHA6A_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8970971.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFS"
BASE COUNT         1528 a          571 c          768 g         1307 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tacgttatgt agtaaatgta atagtttttt tggtagtgag tatgttgagg
      241 aatatattag atttgcagaa gacaataaaa atttccttta taacaataca agtttaaaga
      301 atggtaagtc tgatttaaca cattcaataa agaaaatgaa ttcacttaga gttgcaaaag
      361 caattgtagg aatgtttttt agtttaaatg gggatgaaga ttcaatggat aagccattct
      421 tagattcggt acgagaatat cttagcaatc ctaaaagtac tttatttcct catgaagact
      481 acaaaattat aatgaattat tatcttccaa taggtgctcc acagaattct aaaatcaaaa
      541 ttcctaatga cattgtaaat gatggtgaca ctcacaatgt aataaaaggt tttctgcccc
      601 ttgatctatc aactgggaga aaaattcgat tttcagaaat tcagtatgaa ggaattggtt
      661 ttactcttat agatttaaaa aattcaactt ttgaaataaa agaaggatta gatttaaaaa
      721 atctcctttg tgcagatgat aaaatcaaaa aaatacaatt gtacaaagtc cctatattaa
      781 caccaacagt acttacaatg ttggagatgg aaaataatat taattatatt gactcaggtt
      841 cagatttgct gattaggtgg caggtaataa ctaattttct tatggaatta aaaaaataca
      901 gtaatactca aacgaagttc tcttgaagtt ataaagaata caataaatat aaatttcctt
      961 tagactatat taaaatcagc aatcgaagga aaataaaaga agaaaatggt acgcaaaatc
     1021 taactattag agcaaaaaca ggaagctgct ttggaagata gtcccgaatt gtttccgatg
     1081 ttaaaagatc gtttaaatga attgactgaa aaacgaagac agttacaaat tcgagagaat
     1141 gaaatcttag ggatctttca acaacaaggg gaaccgattc aagtcaaaga tgtccaacag
     1201 atattgacta gtttggattg ctttcttatc cagagtgaga agaagcaagt taaagctttg
     1261 tatcacacct ttattgagaa aatcaccttt gatcctaatc agaagtagat attgccgatc
     1321 ccgatgagat tcacacctac tgtgatttga taattgaatg agtaatatca aatagccgta
     1381 tccaaaccaa aggatatggc tatttttgtg ttagggatat acgtcacgta gcttgttatc
     1441 aagaataagt ttctttattt ttttgctttt tctaaaactg agtatgctaa aataagaggg
     1501 tatatagcaa ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcag
     1561 aagttattta gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat
     1621 tatttattgg ggctgatttt ctataaatat ttatccgacc gtttgttgga acaagtggtt
     1681 cttttagcag atgaatcctt agaggaatat gacacagtaa gcaaacaaac gatgttgtat
     1741 cgtgaattac tttcagatga agaatcaaaa gaagatctga ttgcaacaat cgttgatatt
     1801 ttggggtatg cgatttcgcc agagtatctt ttcaacgtct tagcggatca agcaaaacaa
     1861 gcaacgttcc aattgaatga tctaaacaaa gcctttttag caagtcatat ttatggaaaa
     1921 atactgtcgg caaaattcga atagtgtcga ggaggtgctg tgatggaaga attaaaagaa
     1981 cgcattgtca attatgtcat agagaactct gagattaaaa ataaatgggg gaacaataca
     2041 gcacagaaaa tatctgaaaa attaaatgtt agtcgtaata ttgtctctca atatttgaat
     2101 gaattcgtaa aagatggaga atttggaaaa atcaatactc gtccagtaat cttcttttct
     2161 ttaccggaga aagttccaaa tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg
     2221 tatgatagct cttttagggt tggaatagaa agggttatcg gaagtcaagg aagtctgcga
     2281 aatgtaattg ctcaaattaa agcagcaatc tactatccgc caaatggctt acccatttta
     2341 cttcatggcg agactggaac tggaaaaagc tatttagcaa attatgtgta taaggatttg
     2401 ttggccaata attatataga agagaaggca tctttcattt cagttaattg tagtgaatat
     2461 gccaataatc cagaactttt cttgtccaat ttttttgggc atgtaaaagg tgcatataca
     2521 ggagccgaag atggaagaaa gggtttagca gaattagcta ataatggagt cttattttta
     2581 gatgaaattc atgcattgag caatgaatgt caggagaaac tttttcagtt tatggatacg
     2641 aggaaatttc atcggatggg tgataatgaa aactggtaca catcaagttg ctggattata
     2701 tttgcaacat cagaaccacc tgaggataaa ctattatcaa ctttgttgag aagaatacct
     2761 gtaaagctaa cacttccaac attagacagt cgttctcttt atgaaagaaa acagttaatc
     2821 aagatatttt ttaaatcaga agaagaaaga ctagcaaaga aaatactgat atctcaacaa
     2881 ttattagatt ttttattaaa ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc
     2941 attcaattga cctgtgccaa tgccttttat ttcttagatg atttggaaga actttatatt
     3001 cagatgtatc atttaccaca atgggtgaat ttcaacggca tgaaggttat gaaaagacag
     3061 aatgaattaa aatatattca aatggatgat ttggaaaaag tagacgaagt ttttaatatc
     3121 aacgaactta tagagtatct ttatcaaacg tggaagaaag acactgataa gcagcagctt
     3181 tttgagaaat atgatagata ttttagacaa aagaatgttg atatccaaaa taattcagct
     3241 ttaacatatc ccacctatca agagattcga tttagacagt atgttaagga aatagagaga
     3301 gattttcaaa tagaatttga ctattattat cttcaaataa cgtctatctt tttgaattct
     3361 tggatttatc aaatccaatt aacatcggac gataaattat attccttcat caatgatatt
     3421 aatagttggt gtagtcgaga agttcaatta gtcaatcgtt ttattcaaat aggggatatt
     3481 tatttaacaa aaattcaaaa aaatgttttg gttattttat ttaagttaat gtgtagtaaa
     3541 gaatttcagc aaaaacgttt agcactgatt ttggctcatg gagatctaac ggcaacctgt
     3601 atagcaagta ctgctaatag gatcgttgag agatatatat acgacgccat tgatatgcca
     3661 ctaatgactt caagtagtga gattgcagac aaaattaata gatacttaca agagtgtacc
     3721 ggatatgaag aaattatttt acttgttgat atgggttcac tggagaaaat ttatctaaat
     3781 ttggaactag acgctcaaaa aaaattcgga ctactaaaca atgtttcaac aagaatggct
     3841 attgatgttg caaatcagat attattagat ttaccgctta aacagattct tgaacaagta
     3901 aaagaaaatc aaaatgtgga ttatatttat gaatcatcaa atattaagaa ggatatgatt
     3961 gtctgttctt gtgcaagcgg attaggaact tcgtttaaat taaaaaggat acttgaagat
     4021 agctttccaa gggatactga tcttgtcata gaaacgtgtg actattactc tttagtgaat
     4081 gaaaactatt tatcggagct tcgtaaagag aacaatattc tttttattgt tggaacacta
     4141 aatccacatc tagaagatgt agaattcttt tcaa
//
LOCUS       DAYYHS010000147         4148 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000147 DAYYHS010000000
VERSION     DAYYHS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.151"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..759
                     /locus_tag="ACHA6A_002860"
     CDS             13..759
                     /locus_tag="ACHA6A_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM8970972.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            835..1041
                     /locus_tag="ACHA6A_002861"
     CDS             835..1041
                     /locus_tag="ACHA6A_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM8970973.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            1144..1797
                     /locus_tag="ACHA6A_002862"
     CDS             1144..1797
                     /locus_tag="ACHA6A_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970974.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            1933..2568
                     /locus_tag="ACHA6A_002863"
     CDS             1933..2568
                     /locus_tag="ACHA6A_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8970975.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            2687..3898
                     /locus_tag="ACHA6A_002864"
     CDS             2687..3898
                     /locus_tag="ACHA6A_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8970976.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
BASE COUNT         1592 a          681 c          780 g         1095 t
ORIGIN      
        1 aggaggtata agatgaagca aacgactcag caacgactta atcagttaat gtctgaaaga
       61 aatttaaagc aagttgatat tttgaatatg tctttacctt tgcaaaaaga aactggaata
      121 aaaatgtcaa aaagtcatct atcgcaatat gtcaatggta agtcatcgcc cgaccaacac
      181 aagctttatt tgttggctaa aactttaaat gtgagtgagg catggttgct tggttacgat
      241 gttcctaaag aagataagaa aaataacatt ccatccattg aaacaatcta caatcaatta
      301 gaaaaaccaa gacaaacaaa agtctacaac tttgctgaac agcaactcga agagcaaaat
      361 cgaggaaacg tggtgcactt tcctaaaaag gaaaaacttc cgacaatcaa aaattcagct
      421 tctgccgcca acccgactga attagtttat ggcgatacag ttgtagagga agaagaattt
      481 gaaagagttc caaacaatgc tgattttgct gttcctatta taggcgattc aatggaacca
      541 gttatcagga atggacaatt tgtatttgtt aaggaacagc cagacgtgga agacggtgag
      601 attgctatcg ttgaaataga tggtgacggt gtgacgtgta aagaagtata taaggattat
      661 gagagtcaaa cgattattct acgctctata aacgaattat atgaagatag agtcgtctca
      721 ccggaacaga ttagaataat cgggaaagtc gtattctaaa aaatacccca gtcgtagttg
      781 gcgctccggc taggggttag tatttattat catagtagaa agaaggaaaa gaatatgcca
      841 agttacgttg tattgcaagt tgtattaaaa gaaaagttta taggaaaagg gtcgcaaaac
      901 ctatcagaac ttgaaaacac tataaataga cagtgttcta aaggttatcg attacacact
      961 atttccacta caaacggtgg tagtaaaggt ttcggtggtg gcgatagaat ccaagctact
     1021 ttagttttcg aaagtctata aacaaaaaaa cacgcccacc gaccaaagtt tgtggacgtg
     1081 ggatgttaaa cctatagtag gcttctttac aacttattgt atcaaagaaa agaggagtat
     1141 aaaatgaaaa aagttagcgt tatgttgttg ttaagtactg ctctgctact ttcagcttgt
     1201 tcaaataata aaaaagctga atcaacagat gccacttcta accaagaaac aaaaataagt
     1261 aaaacaaaag aaacaactga aaccagttca tctactagca aatctacatc taaaacagat
     1321 tctagttcaa cagttacaag ctccaaccaa gttacggcgg aacctagccc aacagttata
     1381 agctccagtc agagtacaat ccaaaccgca cctcaagaag aaacatatga acagatgaaa
     1441 caacgcactt tacagtcaac tccagctgat cgtgcaaatt ggtccaacaa agagtgggaa
     1501 gctttcggcg tggcccttta tgaaaatgga ttgactacag atgatgctgg caatattatc
     1561 agtcaagatc agaaagaaca acaagcagca tctcaacaaa atccagaaga ccaacaaaca
     1621 agcgctcagc aagacgctga cactttatca cttactgatt ttgttaacaa atacgggatg
     1681 tcgcctgttg catggaaagt acagaatgga atgtctgaag aagaagcatt gcgtacaaca
     1741 cagcaaaaga cttccggtga agttcaatta ggatttttta aatacggaat tcaataatat
     1801 atttttatgc cctactattt tgcctataat ctctaaaaaa gttataaata aaaaagcccg
     1861 tgctgcaaca cggactcata cctcatttct gagatcacaa atatattata acaagaaatg
     1921 aggaatattt aaatggcaaa aaaagttatg ggtcaagatg ggaaaatgta taaggttaaa
     1981 aaaccgtttt ataaacgggt atggttttgg gtattaatag tacttttgtt ttttggtatt
     2041 ggtggtgctc taagtggcgg tgaaaacaaa gattcagcta ctgcaacatc aacaagtact
     2101 aataaagaaa ctacaaaaga aactacaaca aaagaagaaa agacttataa aatcggtgat
     2161 gacgttgctg tagggaaaat ggaatacaaa gtaaactccg ttgaagtagt aaaacaagtt
     2221 ggcccatctg tacttcctac taacgcaaaa gacactttct tagtagttga cttatctgta
     2281 aaaaatgcag gtgataaggc tgtgactgtt gatagctcat tcttcaaatt aaaagcagat
     2341 ggaaaaactt tcgaagctga ttcggctgca tctatgtcgg caaatcaaga tgaaaatggt
     2401 aatatcacta actctttctt tatggaaagc ttaaatcctg atatgcaaca aactggtaaa
     2461 atagtttttg atatctctga agcccaagca aatgcaaaaa ataatgtatt gcaagcacaa
     2521 acaggttact ttggaacaga gacagtttca attgctcttc acaactaatt agtataaaaa
     2581 acacgcccca ccgaccaaag cgagcgtgtt ctaagaaaga aacaaaccta tacgataggc
     2641 ttctttatag tacctattgt atcagagaaa gagagctgat tcaattatgt cagaagtaac
     2701 aggatactta gaacaagtag acaacgaaac atatagacta agagctgtat taggatacaa
     2761 acctgatggc tcggcaaaaa gaaaaagcaa aactataaaa gcaagtagta aaagagcggc
     2821 atataaagaa ttgaacatct ggttagagca attcgaagga atgaccgatg attcgttgga
     2881 tttgttcaat ataacttttg gtgaatttta tagaaaaata tggctttcag aggccgaaaa
     2941 aaatttagag ccaaagtctt atcataacta taagcgaatg atcgagaata gatttcttga
     3001 taaatttgat tttatccctc taatcgatat caaaccatac atgataaaga aaattgttgt
     3061 taatgctcaa agaatcaata caaaagatcc tgggagaaac tctgataaac ctttatcaag
     3121 aaatacaaag cttcgtatgt tatacgccgt taacaactta tttttgatgg ctaaaaatga
     3181 gtacggagca attaaagaaa atcctgtgga aaatgtaaaa ataccaaaag aaaaaggcgt
     3241 aaaaaagaat atcgaagaac cttattcgga agaagaaatt catgcaatgc taaaagcagc
     3301 atttgaggaa agcatagaaa ttaaaacttt aattgttcta gcatttatta caggagcaag
     3361 acaaggagaa attgcagctt tagaagaaaa agatattgac tttgacaaac aagaaataag
     3421 atttcatcag cggatttcag aagtggatgg aaaatcagat attcggttgc tgccaggttt
     3481 gaaaaatgat gatgatgaaa aaatagtaac cggtcctgct tatctatttg atatgttaga
     3541 tgaattaatc aaagagaata aaaagattcg ttggaaatta aatataaaga aattaaaaca
     3601 ctattttata tttgatacaa aacaagatgg aactttgcca cgtggaagtt atctgtataa
     3661 gaagttcaaa cgattcacca aacgtcacaa tttgcgtcac attcgttttc acgacattag
     3721 acatacatca gctacctatt tgcttagcga tcctaatatg actccaaaag agcttcaaaa
     3781 acgattggga caccgagatt ttaatactac aatgaatgtt tatggccatg tcttacgcaa
     3841 agaaaaagat acagcaacta cagcgtttga aaacctatta aaaaaagata taaaataaca
     3901 ctaaaaaatt atattttcgg gtataaactc gggtaggata tacaaaaact tgggataatt
     3961 tgcggtatat aagcatttaa aaatgctgta aattcgctta tcttcattta aaataccatt
     4021 aaataacgaa ctcttaatca gcgggtcgcg ggttcgagcc cctcacggcc cattgggtgc
     4081 caaacccacg gaacaggtaa atgttggtgc gaaagcattt cgaaaacatt tacgctgttc
     4141 ttttttta
//
LOCUS       DAYYHS010000148         4080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000148 DAYYHS010000000
VERSION     DAYYHS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.152"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..686
                     /locus_tag="ACHA6A_002865"
     CDS             <1..686
                     /locus_tag="ACHA6A_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8970977.1"
                     /translation="DPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            703..1137
                     /locus_tag="ACHA6A_002866"
     CDS             703..1137
                     /locus_tag="ACHA6A_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970978.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1754..>4080
                     /gene="esp"
                     /locus_tag="ACHA6A_002867"
     CDS             1754..>4080
                     /gene="esp"
                     /locus_tag="ACHA6A_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8970979.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1495 a          568 c          776 g         1241 t
ORIGIN      
        1 acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat gttaaaaatg
       61 gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata attccaagtg
      121 ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt gaaaagttag
      181 cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt agagatgctt
      241 tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg agggattctg
      301 ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt tctcccttaa
      361 tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact gtaaaggaaa
      421 tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag aatgaaatgc
      481 atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa ataatgttga
      541 aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat tcttcacact
      601 tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa ttaggtttag
      661 tggacaattt caacaacatt agctgagagg ttttatttta ttatgagact atatcattta
      721 cgtagtttgg aggagttgag gaaattggat tttaacaaaa aatttttttg ggaaatttct
      781 gatttgagtt gtttcgtaga atctgagcgt ctaagtaatg gtaataatag tgtgttaaat
      841 gaatatgaca aagtagtaat taataaagta aaaagtttat gtgaagaagc tagagaatct
      901 atcagtcata ttaatggtag caattttaaa caaacattat ttaatctgat taagctagat
      961 gctaaaatat caagttattt attctttttg atacatgatg aatttatgga ttatcaaaaa
     1021 ttggatcagt taattgaaag agaaagctgg gaagattatt acgtgggtct aactttttca
     1081 gaaaagttaa acaactacaa gttaatagat tataagtatt tgagtgaaag caattaatta
     1141 ttcatgtaga gtactagaca ctttaaatat ggacaattca attataaaga aaaagaatca
     1201 ataatttaat gaggctgtga caaatgtcat tttttgtttc gctaaaaacg agtgaaaaaa
     1261 tgttgataac tccaatcatt cgtttaagat tctaggaatc attcagaaat gctgtgaaac
     1321 cgatacttta taattataaa gaaggtgatt tcaaattccg aagaaggaat ttgaagtcac
     1381 ctttttactt atgtcacagt ctcattcagt aatgcgggat aaggctcctt attccgttat
     1441 ttgaatactc tgaagtttaa tctaagaaag ggtaatattt tgaaaacatg cttcatcgca
     1501 tagaaatgaa cttaatttca gaaagaatac atacatatct ttttatatgt tttcagctgc
     1561 tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact ttttttgttg
     1621 taatgttcat gaaattctct aagtaaatta gttaacataa ggttagtaag attcctattc
     1681 tgtgaataat acgtatcaga tttatctttt attcttacta ggaaagtaat tattaaggga
     1741 gttgatttga ataatggtta gcaagaataa taagagagta tttctagaaa aaacaaagaa
     1801 acgagtattg aagtacagta tcaaaaaatt aagtgttggg gttgcatctg ttttgattgg
     1861 tgttggtctt gtcttgggaa caactgaact agttaaagca caagatgaaa tttctccaag
     1921 cagcccactt gaaacagcga taagctccgt acaagtaggt gacaaagtag cttcggggaa
     1981 tacttttcag gagaatcctg gttatacgaa aaattataac ttttcggatt tacaatttag
     2041 tcctcaagaa ctaactggag acacactaaa aggcaataca attggatttg aagtttacgg
     2101 aaaacataat attgcagcat caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg
     2161 attagctaaa tatgtagaaa aaattcaggt cgatccaaag aaaggaattg gatcttctag
     2221 acggaccttt gtaagaatta atgattcgct gggtagacct acaaatattt ggaaggtcaa
     2281 ttatattcgt gcaagtgatg gattatttgc tggggcagaa acaaccgata cgcaaaccgc
     2341 tcctaatggg gtaattacgt ttgaaaagag cctagatgaa atttttaaag agataggcat
     2401 agacaatctc aagaccgatc gtttaatgta tcgaatctac ttggtaagtc atcaagacga
     2461 tgataaaatt gtaccgggta ttgatagtac gggctacttt ttaacagatt cagatgattt
     2521 ctataatagc ttagatgtat ctgaaaataa tcctgaccag tttaaacatg gatcagtaag
     2581 tgcaaaatat gagaaaccta atactcaaac aaaagatggt tcaggatcca caggtgctaa
     2641 tggagctata atcttagatc ataagttaac gaaaaattat aatttctcat attcatcttc
     2701 cgcaaagggc acgccttggt atgctaatta caaaattgat gaacgattag ttccatatgt
     2761 agcgggtata cagatgcaca tggttcaggc tgacaaagta acatatgatg tttcgtttga
     2821 atccggaaca aaagtagctg atttagcaat cgagcgacgg gaaggtcatg aaaattatgg
     2881 cataggttca ataactaata atgatttaac taaacttatc gattttgcta atgcaagtcc
     2941 acgtccagtc gttattcgat atgttttaca attgactaag ccattagatg aaatcttaga
     3001 agatatgaaa gcaacagcac aagttgaaga aaataaacca tttggtgaag atttcatctt
     3061 tgattcttgg ttgtcggata cgaataaaaa attaatccag aacacttatg gaacaggtta
     3121 ttattatttg caagatattg atggtgatgg aaaccctgac gataaagaag agagcggaga
     3181 cacgaatcca tatatcggga aacctgaatt agaagaagta tatgatgttg acacaacagt
     3241 taaggggaaa gtattcatcc acgagttagc gggaacaggt cacaaagccc aacttgttga
     3301 taaagaaggt actgtattag cagaaaaaac tatcgctcca aatgaaaaag atggggctcc
     3361 aatttcagat actgtagaat ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa
     3421 agatgaatta aaaatccaaa tcgtttctcc aggttttgat aaaccagaag aaggttcaac
     3481 cgttattaag gaatcaccaa aagcggttga taaacaaacc gtggtagttg gatttaaacc
     3541 agatgctaaa gaatcaattc ggaataataa aaacttacct gaagatgcag agtattcatg
     3601 gaaaacagag cctgatactt ctaacgttac tgatagtacg aaaggtattg taactgttaa
     3661 gatcggaaat cgaactttcg acgtggatgt agagtttgct gtaaaagctt ctcaagctat
     3721 ggaaaatgat gcaacatacg tacctataac aacaactcca gaaacgacag ttcaaagtgg
     3781 aaaacctaca tttgataaac cagatgttcc tttagctaaa gatgcctttt caatttcaga
     3841 tgtctataat aagtacttcg gtaatgcaag tgttgatgca aacactggta ttgttacatt
     3901 cactccagct aaaggtgtag gagaatcgga gccgattact ggaacaattc ctattaaaat
     3961 tgtttaccaa gatggttctg taggcacgac cgatttagca gtaactgtaa gtaaagatat
     4021 ttatgaaaat ccaggagaaa acattcctgc aggctaccac aaagtaacct tcaccgcagg
//
LOCUS       DAYYHS010000149         4075 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000149 DAYYHS010000000
VERSION     DAYYHS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.153"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..1658)
                     /locus_tag="ACHA6A_002868"
     CDS             complement(153..1658)
                     /locus_tag="ACHA6A_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8970980.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="ACHA6A_002869"
     CDS             complement(1655..2341)
                     /locus_tag="ACHA6A_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8970981.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVKTNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ACHA6A_002870"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ACHA6A_002870"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8970982.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1123 a          859 c          699 g         1394 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa agggatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgttttaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat attttggctc tataatttat gggactgatg aatca
//
LOCUS       DAYYHS010000150         4067 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000150 DAYYHS010000000
VERSION     DAYYHS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.154"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1157)
                     /locus_tag="ACHA6A_002871"
     CDS             complement(<1..1157)
                     /locus_tag="ACHA6A_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8970983.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="ACHA6A_002872"
     CDS             complement(1160..3409)
                     /locus_tag="ACHA6A_002872"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8970984.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..4009)
                     /locus_tag="ACHA6A_002873"
     CDS             complement(3506..4009)
                     /locus_tag="ACHA6A_002873"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8970985.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DAYYHS010000151         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000151 DAYYHS010000000
VERSION     DAYYHS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.155"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHA6A_002874"
     CDS             85..1434
                     /locus_tag="ACHA6A_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8970986.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHA6A_002875"
     CDS             complement(1562..2152)
                     /locus_tag="ACHA6A_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8970987.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHA6A_002876"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHA6A_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8970988.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAYYHS010000152         4053 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000152 DAYYHS010000000
VERSION     DAYYHS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.156"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1075
                     /locus_tag="ACHA6A_002877"
     CDS             152..1075
                     /locus_tag="ACHA6A_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8970989.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="ACHA6A_002878"
     CDS             1081..2334
                     /locus_tag="ACHA6A_002878"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8970990.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="ACHA6A_002879"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="ACHA6A_002879"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8970991.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1381 a          644 c          883 g         1145 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaa
//
LOCUS       DAYYHS010000153         3966 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000153 DAYYHS010000000
VERSION     DAYYHS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.157"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..482
                     /locus_tag="ACHA6A_002880"
     CDS             138..482
                     /locus_tag="ACHA6A_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8970992.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            483..902
                     /locus_tag="ACHA6A_002881"
     CDS             483..902
                     /locus_tag="ACHA6A_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8970993.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(994..1344)
                     /locus_tag="ACHA6A_002882"
     CDS             complement(994..1344)
                     /locus_tag="ACHA6A_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970994.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1310..2641)
                     /locus_tag="ACHA6A_002883"
     CDS             complement(1310..2641)
                     /locus_tag="ACHA6A_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8970995.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2896..3462
                     /locus_tag="ACHA6A_002884"
     CDS             2896..3462
                     /locus_tag="ACHA6A_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8970996.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3607..3694
                     /locus_tag="ACHA6A_002885"
     tRNA            3607..3694
                     /locus_tag="ACHA6A_002885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3639..3641,aa:Ser,seq:cga)
BASE COUNT         1435 a          686 c          762 g         1083 t
ORIGIN      
        1 atatcgactg aataaactaa gcattggaca ggctgaaaga ctacatttaa aatgaaatgc
       61 gaagaaacaa tagaaaataa agttgcctta gaaccatagt tgaatcacct aacagtcgtg
      121 aagggagaat aagagaaatg gcagaaaaac aaaccgcagg acgagatcaa ttaggagatt
      181 ttgcaccaaa atttgcagaa ttaaatgatg atgttttatt tggacaagta tggtccagag
      241 aagaattatc tgctcatcag cgtagtatca tcaccatatc tgcattaatt tcaggaggaa
      301 attttgaaca attgccagct catctgaaga ttggcaagca aaatggcatt actagtgaag
      361 aaatagcaga aatcattacc catttagctt tttatgtaga atggccaaaa gcttggtcag
      421 cttttagttt agcaaaagaa atctatttag aagaaaatag cgaagataag aaagaggcgt
      481 aaatgatgac aaaacacgaa gaaatacaca atggcgtgat ttttccagca ggtgaaaaaa
      541 acgaagctta cgctcaagta tttaccgggc aaagctatct gaatatgtta gtgaatgatc
      601 ccgaagtttc tgttggtgta ggtaatgtaa cttttgaacc aggttgtcgc aatcactggc
      661 atgtccatac agacggctat caaatcttac ttgtgaccgg aggagaaggc tggtatcagg
      721 aagaaggaaa gccagcaaga cgattaatgg ctggagatgt cgtcgtaacg cacaaaggga
      781 ttaaacattg gcatggtgcg acaaaagaca gttggtttga acatttggcg atcactgctg
      841 gtaattcaga gtttttagaa cctgtaacag atgaagtgta tgaaggattg gaaaatcaat
      901 aatgtcaagc ttgaacagct cctgaaagta gattttaatc gaactttcag gagctgcttt
      961 tttgattcag tcttttataa atagaaaata gggttaataa taggaatgag aagcacgtcg
     1021 aaagatctta atattgataa tcggctgggc atacatgatt tccacgtcat caatcaaact
     1081 acctaacaga tgataatctt cccgatcatg tcggggatta cctaatagtt cttgatagta
     1141 gatttccata ctagggtccc gaggattgtt cagcaaatca gcaagtacat ctaaatcttc
     1201 tggctgagtt tctgccgtac cctgtatctc tatgaaaagc ccttcctcag caaattctac
     1261 tccaatacgt atttcagtta tttcatggga atataaataa gtaagaagct catcgatgat
     1321 tctttttgat aattcttttt ccaagtgtct ttttttcctt tctgacagcg gtaaagttat
     1381 ctagtatcgg tacaaggata cctgcaacga aaccagttga aaaaccactg ttatatagat
     1441 ttagtcctcc gtgcaaagtg ctgacgttat ggacaagagc aatatgtagc acaccagcta
     1501 ttacaccgta aaaactgccg taaaaaccgc ttatgggcgc aagacctgtc ccaaacacag
     1561 ctgcaacaag catgccagta ctggtgatct catgaaaagt tccaaacaaa gaagcaataa
     1621 atattccgac tagtatcgga aaactgtttt ttaaatgaca accaaaagca ctgaaaccca
     1681 ccgcagataa aatagcaccg atgacaggtc catttaactg accgccaacc agcaaaacat
     1741 aagaagacag catcagacca actaagccca tgttgatcaa agtagcacca ataccagaat
     1801 tagcaatgaa atcagttgtc aattttccag atgaatcgaa aatttctcga atccccgaaa
     1861 gccgaaagga attataatag aaaccaattc cgactgtcag cgaaaaaaag ccaaaaataa
     1921 tccaaatgag aggaaagtga tattcattga aaaccaatgt attttcgacg attgaatagc
     1981 tgaataacct caaaacagca gtaaaagtca tcgcaatcaa cccagaggtg aatccaatat
     2041 tataaagtgt gaatccacga tggaaaacta aagtttgagc agctaagggc ggaagaagta
     2101 taccaattgc tatgccagct aaattcccca ataaaatacc tatcagtaaa ggaaaacgca
     2161 taccaaaagt gatataacta acgaccggtg ataatacgct accaaacagt ccgatcatga
     2221 tgtactgaga aaaaggttta ttgacaaata cagcgtatag atacacacct aaaatgattg
     2281 agatagaatt ataaaggttt ttgccaaaaa aagagaaacc ggtaactgtt agaatcgaag
     2341 caaataaggg accgtttaaa atcaccttgt gccgataagc taaaagcaca gaaagtaagg
     2401 tcaaaatccc tacatttatg aatgcgcttc cgaaaacacc taaagcaaaa taatctgtca
     2461 aaagattgct tggcgaggta agaatagtta gcaatccact ccaattatca gtaggagtat
     2521 tagaaagcaa gccaataaaa agaaaaaaaa cacctgtaaa accaagataa aaatactttc
     2581 ttttctgtga aacgattcgt ttttgaggat gaatataaat aacgtcagtt tcgttttcca
     2641 ttacacacct tctttttcat tttctttaac tataacttga aatgaaagat ttggacaact
     2701 atcatttagg tcaaaactaa aaatgtaagg gaacccattg aatttaacca aattttttta
     2761 attttgcaaa aaaaagttaa agaagactga aaaaaattta aaatataacg ttttgagtaa
     2821 aatgtttgca aaaaatgcta aaaatagggt acaataaaac aacatatact gcagtaaaga
     2881 agtgggggag ttttaatgag aagaaaagtc tataccaagg atcaaattct aaaagctgct
     2941 tatgatgttg ttgcaaagga agggtttaaa ggatttactg cgagaaatat tgctaaaaag
     3001 atggggattt cgacacagcc gatttatctg gaattcaaaa acatggaaga tttaaaaaat
     3061 acgttgctgg attctatctt tgaacaacta aaagaagatg ttttccctat tgaacgtact
     3121 ggtgataaaa tcattgatct cggtttgaac tatatctact ttgcaaaaaa gcatcatagt
     3181 ctttacatgg cattgtacat tgatgaacat ggggacggaa agaaaatgca tgatttttca
     3241 tacaattatt tccgtgaaat agttaaagaa gatgaaagat acaatcaatt atcggatgaa
     3301 tatgtcgatg ctctgcatac tggtacatgg gtagcagtga ctggcttagc gtcattgatg
     3361 tcatcaaaca tcatgcatcc aacagaagaa caaatcattg cactgatcaa acggacaatt
     3421 gactcgattt tagcattaga taatcctgaa ataatcagat agtgttctat cagtatgaat
     3481 ttgatcggta agtccgttgg ctgcaaactg tcaatggact tttttgctaa aaaaagcgat
     3541 ttctgttatt tatctattag aaataggctt gacatttcgt gaaaaaagtt taaaaatagt
     3601 acttacggaa ggttggcaga gtggtaatgc accggactcg aaatccggcg aaccggctaa
     3661 taccggcgcg caggttcaaa tcctgtacct tccttatatc aacgattaga ggttgtgaag
     3721 aagctgtcct gcatcaagta ataagaacgg ccaaacaaaa atagcttttc ctattttttc
     3781 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaataagg
     3841 ggctgtgaca agacttttgt cacagcccct tattgttgta cttttaacct acaatatttt
     3901 ttgattcccc ctgagtacta ttttttataa atgctagtaa atcaatcttc tttggccttt
     3961 tttttg
//
LOCUS       DAYYHS010000154         3906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000154 DAYYHS010000000
VERSION     DAYYHS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.159"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..606)
                     /locus_tag="ACHA6A_002886"
     CDS             complement(64..606)
                     /locus_tag="ACHA6A_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8970997.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            741..1130
                     /locus_tag="ACHA6A_002887"
     CDS             741..1130
                     /locus_tag="ACHA6A_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8970998.1"
                     /translation="MKTVNRVFCASHVLSFLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1134..1898
                     /locus_tag="ACHA6A_002888"
     CDS             1134..1898
                     /locus_tag="ACHA6A_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8970999.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2027..2974
                     /locus_tag="ACHA6A_002889"
     CDS             2027..2974
                     /locus_tag="ACHA6A_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8971000.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3104..3844
                     /locus_tag="ACHA6A_002890"
     CDS             3104..3844
                     /locus_tag="ACHA6A_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971001.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1295 a          665 c          799 g         1147 t
ORIGIN      
        1 aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt ttaagtttgt
       61 gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg aaatatattt
      121 tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc gatacagttc
      181 acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt ccagccccat
      241 tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca aacttttatc
      301 ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg ccaatttgtc
      361 ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga caaataaatt
      421 cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa tcgtgatctc
      481 gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca tgaaacgtaa
      541 cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca tttttggatc
      601 aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa tcgtaagtga
      661 agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat agaattataa
      721 agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc agccacgtgc
      781 taagtttcct tctcagtata tacttaggtg tctattcgat tcaacagcca attgaggtag
      841 agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt tttttctttt
      901 tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat ttttctattt
      961 atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc ttatttcccg
     1021 agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag acaactgttg
     1081 gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa caattgaatc
     1141 caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt ctgaaagaat
     1201 taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca agtaaggagt
     1261 tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc ctgccagatg
     1321 cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat acattttggg
     1381 atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta gaaaaaagag
     1441 gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg acaacatgcg
     1501 ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt tcccctgcac
     1561 ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat gtcgtaccaa
     1621 aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg cagccggaaa
     1681 agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa atcccttata
     1741 tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa gaaattgttt
     1801 ttcgttcctc aaaaacacag cgacatatgg tacaaataca actaatggaa gaagcttcca
     1861 cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa atcactcatg
     1921 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
     1981 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
     2041 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
     2101 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
     2161 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
     2221 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
     2281 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
     2341 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
     2401 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
     2461 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
     2521 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
     2581 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
     2641 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
     2701 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
     2761 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
     2821 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
     2881 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     2941 tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg
     3001 aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt
     3061 gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg
     3121 aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa
     3181 gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt
     3241 tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg
     3301 tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg
     3361 aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa
     3421 actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca
     3481 tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa
     3541 atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt
     3601 aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc
     3661 gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt
     3721 agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg
     3781 ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa
     3841 ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttcc
     3901 ttattt
//
LOCUS       DAYYHS010000155         3885 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000155 DAYYHS010000000
VERSION     DAYYHS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.160"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..327
                     /locus_tag="ACHA6A_002891"
     CDS             127..327
                     /locus_tag="ACHA6A_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971002.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            343..522
                     /locus_tag="ACHA6A_002892"
     CDS             343..522
                     /locus_tag="ACHA6A_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971003.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            847..1110
                     /locus_tag="ACHA6A_002893"
     CDS             847..1110
                     /locus_tag="ACHA6A_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8971004.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            1104..1454
                     /locus_tag="ACHA6A_002894"
     CDS             1104..1454
                     /locus_tag="ACHA6A_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8971005.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1478..2092)
                     /locus_tag="ACHA6A_002895"
     CDS             complement(1478..2092)
                     /locus_tag="ACHA6A_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8971006.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            2406..2828
                     /locus_tag="ACHA6A_002896"
     CDS             2406..2828
                     /locus_tag="ACHA6A_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971007.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2838..3041
                     /locus_tag="ACHA6A_002897"
     CDS             2838..3041
                     /locus_tag="ACHA6A_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971008.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            3252..3836
                     /locus_tag="ACHA6A_002898"
     CDS             3252..3836
                     /locus_tag="ACHA6A_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8971009.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1328 a          585 c          712 g         1260 t
ORIGIN      
        1 acaaaaacct tgaaattaag ggaatggttc ttatttcgca ccaatcgctc caaccgcccc
       61 cgccgcttag gtggtgcgaa ataagcgggc aaagcccgat aacctaagag aaagaaggag
      121 atccatatga ataaaatcat tcctctgttg ttcgtactgt gtattggcga gatacttatg
      181 cgtcatggtg cagctgtaca tttaaacttc gtagtattct taggaatact agtattttgt
      241 ttgatttttt ggcgctttgt gttagccctt gtgagtctac tattcaagtt gattgcagcc
      301 atatttttca tagggttatt tcgatgaaag gagagagtac taatgccaat tgcaatagga
      361 aataaacggt tacccgtgac attagatgaa aagagacaaa aagaattgca gcaactaaag
      421 cagaagtacg gaaaaagtga atctaagatt atgtgtattg cgttagatct attgatcgcg
      481 caagaaaaag caggatttga tataccagca ctaaaaaagt gacgtcactt ttcatttgaa
      541 gaaacaaaag tgatagcact taattgttcc tactatttag aatattttct atgtcctttt
      601 tattccattt tgtatcaata actatgaaat ctttaaagta tccagatcaa tttttttctt
      661 acctgattct aaagattttg ttttcttaaa catattatga tttcctttcg actattttca
      721 tataaatgtt aacatagttt atggattcat ggaatgttaa tctaatttaa tagaaaagga
      781 taattaagta tatacgtttt ttcttatgta tgttatacta tgtatataca gaaagaggtg
      841 tctactatgg aaatgttacc tataaaaaaa tggggaaata gtaatggcct tagattgcca
      901 aagtatatta tggaatatct tggaattcat acagaagaca aagtgaagat aatacaagaa
      961 gaagtaaacg gtcagaagcg gctaattatc gaagcggcta atatagaaaa tgaattaaca
     1021 attgaacagt tatttgaaaa ttacactgat gaaagaaatc atgtgacgat tcaaaattta
     1081 ggtgaagcag taggtaatga aaaatggtga gaaaacctag acaaggcgat attctattat
     1141 tgaatacagc acctcgatct ggacacgaac agacaggtaa aagaccttat attgttttaa
     1201 gtcatgatat tattgcggat tatagtaacg ttgttacagt tgctccaatt tctagtacaa
     1261 ctagaaacta tccgctatat gtaaatataa actcagaata tgaaatgaag acaacaggaa
     1321 aagtattatt agatcaacta acgacaattg attatgaagc cagagagtgt atttttttgg
     1381 aaaaagcgca tgatagttta gttgaggagt tattgatgaa agttagaaca gtttttcaaa
     1441 aagtaagcaa ataaaataga aacagcagca tgatcaacta ttcgtgattg accatgctgt
     1501 ctttttttat tctatatacg gtttgtctcg taacattgta gtcttttgca atcttagcga
     1561 tcgcaacacc tttttttaga tcctcaacga tgtttttata aacaagacga cgttggggat
     1621 cttttgcatt ggcactgtat agcttaggac gtcctttata aacaccgttc gcttttgcaa
     1681 tcttaatgcc ttgtgcctga cgtcgtttag attcggtacg ttcttgttca gcgatcatgg
     1741 ccaaaatttg aatgatcaaa tctttaatga atttatccag gagaggattg ccaattgctt
     1801 cggccatgat cggtagactt gtgatgatca gctgcacatt tttcttcttc aagtaattga
     1861 ctgaagcgat gatctcgtcg taatttcttc caagtcgatc aatagcttcc acaataaaaa
     1921 tatcttgatc tcggacgaaa tccagtgctt cttgaaaaac aggtcgatga gtaatcgttg
     1981 cgccagattg tttctcaatg aaaattctct ctgcaccgaa ttttttcatt tcttcaatct
     2041 gccgttgttc attttgatcg atggatgaga cacgtatgta tgcaactctc aaaattttta
     2101 cctactttct gcaactattt aaaaaacacc ctaatcatac acgagaaaac cagatttttc
     2161 aatgtcgcgg tcgtgtctag ttagggtata ccttatatag acaaaaacta atgatggagg
     2221 aaatagtatg tgtcactttt ctttttaaat ggcaatattt tttgtaaata aatgtcaatc
     2281 aagaaaattt aaacgattat gacattaaaa atatatttta tgaattttct agtatataac
     2341 ttgatttatc tgttaagatt tattaagaaa acgttttctt atttttgatt tcgaggtgat
     2401 agacaatgaa aaataagttt attttatctt taggtatttt aactattgga attagtgtaa
     2461 ctggaactac aattcctaca tttgctgata gtacaaatga accaactact gtagtagata
     2521 ataaccaaga attaactcct ttagatagga acggaatagc cattggtaac ccaactaacg
     2581 acttaactgt tactgaaaat tatttaatag tttctccatt acttgctcga gcagcttcta
     2641 aaacgatacg agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc
     2701 ctcaatcacg gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt
     2761 tacaaagtta taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta
     2821 gaaagtaggg attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg
     2881 atattctaag taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt
     2941 tacaggagac acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa
     3001 gaaaaaaaag agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg
     3061 tctagtcgac attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca
     3121 ctttcttttt ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa
     3181 caatatctat gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag
     3241 gagtgatttt tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt
     3301 tacaagaaga cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg
     3361 gggcaaaaag taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata
     3421 cacttgtcgt ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg
     3481 tcaatcgact gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg
     3541 acaaatcaag ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg
     3601 agcgtaattt gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac
     3661 ggtttggtgg tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag
     3721 tggatagcgg tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca
     3781 tttatcgtta tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt
     3841 tgtcatttaa tgctgaaaat ttttgccatt tatctagaaa agtga
//
LOCUS       DAYYHS010000156         3878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000156 DAYYHS010000000
VERSION     DAYYHS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.161"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(718..3867)
                     /locus_tag="ACHA6A_002899"
     CDS             complement(718..3867)
                     /locus_tag="ACHA6A_002899"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM8971010.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DAYYHS010000157         3847 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000157 DAYYHS010000000
VERSION     DAYYHS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.162"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHA6A_002900"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHA6A_002900"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8971011.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACHA6A_002901"
     CDS             complement(2261..2512)
                     /locus_tag="ACHA6A_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8971012.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACHA6A_002902"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACHA6A_002902"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8971013.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACHA6A_002903"
     CDS             3462..3680
                     /locus_tag="ACHA6A_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971014.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1064 a          817 c          714 g         1252 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtcttt
//
LOCUS       DAYYHS010000158         3836 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000158 DAYYHS010000000
VERSION     DAYYHS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.163"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..331
                     /locus_tag="ACHA6A_002904"
     CDS             <1..331
                     /locus_tag="ACHA6A_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971015.1"
                     /translation="LLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSIS
                     LCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNF
                     YFYRNKL"
     gene            complement(457..1134)
                     /gene="nth"
                     /locus_tag="ACHA6A_002905"
                     /pseudo
     CDS             complement(457..1134)
                     /gene="nth"
                     /locus_tag="ACHA6A_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1141..1839)
                     /locus_tag="ACHA6A_002906"
     CDS             complement(1141..1839)
                     /locus_tag="ACHA6A_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8971016.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2107..3291
                     /locus_tag="ACHA6A_002907"
     CDS             2107..3291
                     /locus_tag="ACHA6A_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8971017.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3297..3740)
                     /locus_tag="ACHA6A_002908"
     CDS             complement(3297..3740)
                     /locus_tag="ACHA6A_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8971018.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          998 a          759 c          635 g         1444 t
ORIGIN      
        1 acttttaagc actactctta aaggaaaatt tatggaaaga agctctcatg caatgattct
       61 cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc tgaaaccgcc
      121 caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac ttatcatttt
      181 tattacacct ttagatagat acgtcactta tttatataat cctttggaat actggactta
      241 ttttataggt atattggaag gcattatttt ctacatcggg tattttccca ataaaaataa
      301 caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta ctgagtatgt
      361 acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata ataacaagat
      421 aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt tcttttttag
      481 cgccttttct tttagacgca tccgattttt cccatcttga catattgata gcaaaggaca
      541 tacctcgcac ttgggatttc ttgccgtgca atgatatcaa ccaaaaaaaa tcagtgtatg
      601 atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt ctacttccat
      661 gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca cgtgggtatc
      721 tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg tctttctgcc
      781 tacaccagga agagacatca attcttctct tgacgttggc acttgcccat cgaaacgttc
      841 aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca aaccaatcgt
      901 ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg gtgtcggaaa
      961 agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct gggcacttaa
     1021 gatcactgca atcaataact caaatggatt attgtgcttg agttctccat gtgcttcggg
     1081 aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta acatattccg
     1141 ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt gagctgactg
     1201 ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt ccacttgttg
     1261 ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt aattaaaact
     1321 ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg gctgataatg
     1381 gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg ggcgtccaaa
     1441 ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt tttcttcgta
     1501 ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt aaagattata
     1561 tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc catttgttac
     1621 gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca tagcaatcat
     1681 ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta gaacaaataa
     1741 aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat tagatatgac
     1801 tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc cgctcaatca
     1861 cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt tacttcactg
     1921 atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt ttgatgcgta
     1981 tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga atgttgacaa
     2041 ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc ataaaggagt
     2101 ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt ctctgtctta
     2161 ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac gctgccttat
     2221 tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt ttatgctcta
     2281 gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg gcgtcatccg
     2341 atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg tttaggaaat
     2401 tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg agaaatctcc
     2461 accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa agtttttgga
     2521 tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg gggtctatta
     2581 gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt cttaaatgcc
     2641 gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc agtagattta
     2701 agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat ttcatccgtt
     2761 attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct tcaatctatc
     2821 ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg tttaagcttc
     2881 tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg attgctcaaa
     2941 aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct cgcctatcta
     3001 ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg gattatctgt
     3061 tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc caattctgtc
     3121 tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc
     3181 gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct tccgacggta
     3241 ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata gtagcttcat
     3301 ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt gtcaaattct
     3361 tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg atggagttgc
     3421 tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta
     3481 ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt tgttagctta
     3541 ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg
     3601 attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc aaattctttt
     3661 tccatttctg gtttattacg atgaataatc tcattagcta aatccagata ttcttctatt
     3721 agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt gtttttttgt
     3781 ttcctattca acaaagataa aaatattttg taggaaaacc caatctgaaa ccaaga
//
LOCUS       DAYYHS010000159         3740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000159 DAYYHS010000000
VERSION     DAYYHS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.166"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1108
                     /locus_tag="ACHA6A_002909"
     CDS             134..1108
                     /locus_tag="ACHA6A_002909"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8971019.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="ACHA6A_002910"
     CDS             1090..1551
                     /locus_tag="ACHA6A_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8971020.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVCIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="ACHA6A_002911"
     CDS             1601..2065
                     /locus_tag="ACHA6A_002911"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8971021.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="ACHA6A_002912"
     CDS             2079..2828
                     /locus_tag="ACHA6A_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8971022.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="ACHA6A_002913"
     CDS             2828..3658
                     /locus_tag="ACHA6A_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8971023.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT         1259 a          488 c          721 g         1272 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa atttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtattgtaaa gttatatgac tttataatac ttattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtatcaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttgtat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgacttc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt
//
LOCUS       DAYYHS010000160         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000160 DAYYHS010000000
VERSION     DAYYHS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.167"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHA6A_002914"
     CDS             complement(<1..353)
                     /locus_tag="ACHA6A_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971024.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHA6A_002915"
     CDS             complement(613..1161)
                     /locus_tag="ACHA6A_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971025.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHA6A_002916"
     CDS             complement(1162..2019)
                     /locus_tag="ACHA6A_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8971026.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHA6A_002917"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHA6A_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHA6A_002918"
     CDS             complement(2581..2910)
                     /locus_tag="ACHA6A_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8971027.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHA6A_002919"
     CDS             complement(2987..3259)
                     /locus_tag="ACHA6A_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8971028.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHA6A_002920"
     CDS             complement(3259..3522)
                     /locus_tag="ACHA6A_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8971029.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYHS010000161         3717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000161 DAYYHS010000000
VERSION     DAYYHS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.168"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHA6A_002921"
     CDS             72..155
                     /locus_tag="ACHA6A_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8971030.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHA6A_002922"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHA6A_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8971031.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHA6A_002923"
     CDS             complement(1280..2143)
                     /locus_tag="ACHA6A_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8971032.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHA6A_002924"
     CDS             complement(2145..2417)
                     /locus_tag="ACHA6A_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8971033.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHA6A_002925"
     CDS             complement(2434..2643)
                     /locus_tag="ACHA6A_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8971034.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACHA6A_002926"
     CDS             complement(2721..3617)
                     /locus_tag="ACHA6A_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8971035.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DAYYHS010000162         3671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000162 DAYYHS010000000
VERSION     DAYYHS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.169"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHA6A_002927"
     CDS             64..972
                     /locus_tag="ACHA6A_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8971036.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACHA6A_002928"
     CDS             972..1592
                     /locus_tag="ACHA6A_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971037.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="ACHA6A_002929"
     CDS             1596..2045
                     /locus_tag="ACHA6A_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971038.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..2539
                     /locus_tag="ACHA6A_002930"
     CDS             2045..2539
                     /locus_tag="ACHA6A_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971039.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            2553..2984
                     /locus_tag="ACHA6A_002931"
     CDS             2553..2984
                     /locus_tag="ACHA6A_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971040.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2986..3123
                     /locus_tag="ACHA6A_002932"
     CDS             2986..3123
                     /locus_tag="ACHA6A_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8971041.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            3160..3453
                     /locus_tag="ACHA6A_002933"
     CDS             3160..3453
                     /locus_tag="ACHA6A_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971042.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            3450..>3671
                     /locus_tag="ACHA6A_002934"
     CDS             3450..>3671
                     /locus_tag="ACHA6A_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8971043.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT         1320 a          546 c          813 g          992 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa ttcaagttat ggatcagctt attcatttgt
     2521 tatcacttat tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc
     2581 cttacggatt cgagcaaatc attttgaaca atcaagaaaa tatccctgaa aactgtacag
     2641 agattaaacc accagttcct aactggagac caagatttga ttttgataaa aaacagtggg
     2701 ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt tgatagcgta gatgaattgg
     2761 ctaatattaa ggcattatac gaaacgctga aggcagaaaa tgatgaattg aaacagctaa
     2821 attctaaagc aatgctaaac aatgtagcaa tcaagcaaga aaatgtctta ttgaaagaaa
     2881 agtcagaaag tttagcgcag ttgaattcaa aaacgatgct tgcttctgta caaaatacca
     2941 aggaaatcga agaaatcaaa aaacaattac aaggtgggaa gtaacatgta ttcatatgaa
     3001 gatatcaaac taatgtatga ctggggctgt ttcactaacg aacaagtaat ggttttcgtt
     3061 ccattatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
     3121 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
     3181 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
     3241 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
     3301 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
     3361 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
     3421 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
     3481 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
     3541 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
     3601 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
     3661 gaggaaacaa a
//
LOCUS       DAYYHS010000163         3554 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000163 DAYYHS010000000
VERSION     DAYYHS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.170"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            371..1234
                     /locus_tag="ACHA6A_002935"
     CDS             371..1234
                     /locus_tag="ACHA6A_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8971044.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1334..1816
                     /locus_tag="ACHA6A_002936"
     CDS             1334..1816
                     /locus_tag="ACHA6A_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8971045.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2149..3450)
                     /locus_tag="ACHA6A_002937"
     CDS             complement(2149..3450)
                     /locus_tag="ACHA6A_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8971046.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         1148 a          594 c          754 g         1058 t
ORIGIN      
        1 gaaataaata attgttccaa ttgataaaag aggtatgatg tatcgcaaag aaaaatccaa
       61 gcagcgttct gatcagcagt ttgaacaaca atttgctttg tacagtaggt agtaagcgga
      121 ttaactaagg gaataaaaga tatttttacc gctttgttta cttagaatgt gtcttcccca
      181 aaatttattt ttctaaaggt aaaacataga taggcagata ataccaatta tccgttacga
      241 aacgctgctt gagaattttc tttagatcca tacaacattt tctacatctg actaaattgg
      301 ttcctgattc atcaagtaat tgactttctt tgataatatg ataaaaaggc aagcaaggag
      361 gaagataaat atgcttaaag atctgaacca attgatcgat tacatcgaag aacatttgac
      421 agaaggactt tcctataaaa taatagccga aaatacagga atctctgaat atcaattaaa
      481 acgcatcttt acgtttattg cagggatgtc gctaatggac tatatcaaaa accgtagatt
      541 ggctttggct aatcgcgatc tcctagatgg atcaagagtg acagaaacgg cgtttagata
      601 tggatatgaa acagtcgaag gtttttcaag agcttttcgt gattggtcag gatatctgcc
      661 ttcagaaatc agcagatacg gcatacaaaa atcttttcca cgcttgacat tttttattga
      721 tgtcagagga ggaaaatcga tggaatttcg tatcgaaaag aaagaacagt tttatgtggc
      781 aggtgtggcg aaaaaagttc ctgtcaaatt tgaaggagtc aatcaagaaa tacaaaagtt
      841 agcgcagacg atcacggaga ctcaaaggga agagatgcat aagttaggag atttatatcc
      901 aaatcaagta atcaacgttt cttatggatt tgatggagcc cgcttggaag aaaagggcag
      961 tttgatgcac atgattggct ttgctacgac aaaagaaaat ccgtacgctg atttagaaat
     1021 gcttgctata gaagaacaca cttgggccat ttttcctaat aaagggccat ttccccaaac
     1081 tttacaagat acttggggga aaatctatgc agaatggctt ccttcttcag attatgagtt
     1141 agtcgaagct cccgagatct cttttacaaa atggggagag gatttcagta atgtttacag
     1201 tgagatttgg attgcagtga aagcaaaaaa atagttaaaa aataaaaata tccctagaaa
     1261 aaagagcgta atcgtgtcat ttccctttaa ttttatgtaa gatgaactta atataataga
     1321 aagggtggga gaaatggcaa agtataggat cgaagaaaaa ccagatttta cggtgacggg
     1381 tattggtgtg gaattgaaga gtagcaatac ggattttgtc ggtttggctg cagagaaaca
     1441 agcatttgag caaaaagtta tcggtaatgg aacaatagat gcattgaaac aagctgctga
     1501 aaatcattat ttctttacag tcaacgaacc ctataacggg aaaatgatgt actatgtcgg
     1561 tgttcaaacg agtcaggatg ttccaaacac tacccgaatc atcccattcc caaaaggaac
     1621 gtatcttgtt gtaatcgctt tagcagaatc gcctgaagaa ttaagaaatg atttgacgaa
     1681 tacggccttt gggcatgttt taagagaagt agaagattac gcctatgttg ggggtccaaa
     1741 tgctgtagtc gaaatgggag ttaccaacgg gaaatatatg ggagaaatct ggataccggt
     1801 agtcaataaa ccatgataat atagaggtcg tgaaagtagc gctttgacct gagcattaga
     1861 gaggaaaaat gggaaatagc tttccatatt ttgcattttt cagaactaat gccgagggtc
     1921 agctacttga acaccgttta tcaagacgtc atgacttcag cgctttgacc tgagcattag
     1981 agaggaaaaa ggggcatgac aaggcttttg tcccccggag aataaaagaa tttatgtaga
     2041 cgattccgat cagaaaaatg caggcacagt agagagaatt atggctcttt gtcaaatagg
     2101 actgatagag tgagataaga acatctaagg tagaattcct tttctacttt agatgttttt
     2161 tgattcatag gtaatcagat tatttagtaa gaaaattctg gtcttcattt tccgaaagct
     2221 tcgaaagcca taagcgactc gtttcaatgc tttgatgtgg gtatgcagat tttctatttt
     2281 ggcatttgaa taaggcaatt cgagggctaa acgaatgccg gattcatagt ttataaaagc
     2341 tttttttata gaatgcagtt cttttggaac ggaattgggc atcgttctta caagtttaaa
     2401 gaaagtgtcc gcctctttgt cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc
     2461 gattcgtaat tcgtcagaga tcgaaagcag acgatccacc acttcacttt cggtgagata
     2521 cgcccaacca aaattgcgcc atttggtcca tttgtcataa acaagcttgt cctgtcgttt
     2581 gatcagaagc cgagacaacg cctttaattg tcgataatat ttggcttgtg ttggatcgtt
     2641 tcgatcaaag cttttcatga ttcgtacgcg aatatcctca aatttacgaa tgagatgttt
     2701 gacaatgtga aaacgatcca caacgagttt tgcctttgga aaacattcct ttatcagaga
     2761 ggcataagaa acattcatgt ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc
     2821 aaattgcata aaataagaaa cgagagcctt tttcttccgt gaaggaagaa tatccagtaa
     2881 ttttccagta tcgccatcca tacaagaaaa ggagtaggga ccgacttgat ttttcagtga
     2941 acgaaattca tccattaata aacaacgcgg taaccaattt ctttttactt ttgtcatggg
     3001 gatcagagaa gcgagtgtcc gagaaacggt cgaccaagaa acgccgcaat gcttagcaat
     3061 tgtcgtcatt gcttgttttt cagttagctc ttctaaaatt ttatagcgca cttgtttagc
     3121 gataaagcag tttctatcga caatgtaggt attcgccgta aaataggtgt ggcagccacg
     3181 acagtggtag cgttgtttcg ctaaagcaag ataagtcagt tggttcgcac atttattaag
     3241 ttgaatgagc gattttttct ttccgttctt aacgatcatg ccttcatgat tcatctgttt
     3301 acacaaagga cagcgacgaa tttctttact taacgtcgca gaaacaacaa gactcttgat
     3361 attcttaatg ctcttcgatt ccacatttgt gatgaaaatg tttttatctt tgattccaag
     3421 tagtagtttg atatgatgaa gatcaggcat acagaacgct cctttgattg tgttgtggtg
     3481 attcaattct aacggagctt ctgtatgttt tgttttttca tgctcaaaag ggaccgatag
     3541 aatgattttc tatc
//
LOCUS       DAYYHS010000164         3529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000164 DAYYHS010000000
VERSION     DAYYHS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.171"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..488
                     /locus_tag="ACHA6A_002938"
     CDS             <1..488
                     /locus_tag="ACHA6A_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8971047.1"
                     /translation="ILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYI
                     TNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLN
                     ENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIA
                     Y"
     gene            461..1138
                     /locus_tag="ACHA6A_002939"
     CDS             461..1138
                     /locus_tag="ACHA6A_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8971048.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1089..2270
                     /locus_tag="ACHA6A_002940"
     CDS             1089..2270
                     /locus_tag="ACHA6A_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8971049.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2283..3260
                     /locus_tag="ACHA6A_002941"
     CDS             2283..3260
                     /locus_tag="ACHA6A_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8971050.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3260..>3529
                     /locus_tag="ACHA6A_002942"
     CDS             3260..>3529
                     /locus_tag="ACHA6A_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8971051.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEK"
BASE COUNT         1328 a          438 c          666 g         1097 t
ORIGIN      
        1 aaattttagc tttgatcaat tcaatggaac aacaatctca accaattatt tgcattttac
       61 ctaatagtga tgctggtagc gaaattatca gaaaaatgta ccagaaatat ttaaaaacgg
      121 atattattta tattacgaat ttagagccgg aggaatacct gactatatta aaagagtgca
      181 aatttatgat cggaaattct tcttctggga taattgaagc tgcatcattc aaaataccag
      241 taataaattt aggaaccaga caaaatggac gagaacaatc taaaaatatt ataaatgcaa
      301 atctaaatga aaatgaaatt gaaactgctt tgaataaaat acaagatcct cagtttataa
      361 aaagcttgaa aaatgttaag aacatctacg gtgacggcaa cagttctaga agaataacaa
      421 aaacattgaa tcaaatattg aatgatgggg aagatattaa gtggattcaa aaacaaatag
      481 cttattaaaa tatactagtg atccatacat tattattggg gctggcggac acgcaaaagt
      541 cattattgaa accttaagat tagaaaataa gttaatatat ggattttttg atgataagca
      601 aaataaaatg caagatctga aatatcttgg gaaaataaac gaattgaata gctgggcgga
      661 tagcaaaaaa aaaatctata aatttattgt agctattggt gactgtgtag taagaaaaaa
      721 gattataaaa aaattgaata tgccaagtga gcaatatgga atagctgttc atccaagtgc
      781 tatagtggca aaagaaattt caattggtta tggaactgtt atttttgcga atgctgtaat
      841 taacccagat gctgtaattg gagagcatgc tataataaac acaggtagca ttgttgagca
      901 tgattgtaga attaataact atgttcattt atctccaggt gtatgcttag cgggtggagt
      961 acatgtaggt gtaggaactc aaataggaat tggttctcaa tgtatccaga tgaagaaaat
     1021 tggatcttgg tgtatgatag gcgcagggag cactattgtt aaggatattc cctcacattc
     1081 cttagcttat ggcaatccag ctaaaattaa gaaagaagga attaattttg aattctagaa
     1141 ttttattatc aagtcctcat atgggaggaa cagaagaaaa atatgttgct aaggctttta
     1201 aagataattg gatagcgcca ttaggtgaaa atgtggataa atttgaagaa agtattacaa
     1261 aatatactgg ttctaagggc ggagcttctg cgttaagttc aggtacagct gccatacatc
     1321 ttgccttaag actgcttaat gttggaaaaa acgatattgt ctttgctcct tcatttactt
     1381 ttatagctac agtaaatcct attttatatc aaggggctgt accagtgttc attgactcag
     1441 aacctaatac atggaatatg agccctgttg cactagagag cgcattagaa gaagctgaaa
     1501 gaaacaacca attacctaag gcaatcatcg tagtgaattt atatggtcaa atggctgatt
     1561 atgaacgaat tttagctatt gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg
     1621 aatcgttagg ttctacttat aagggaattt cgtcaggctt atttgggaca attgggatct
     1681 attcatttaa cggtaataag atcatcacaa cctcaggtgg aggagctcta gtgtctagtg
     1741 ataaaaaata tgttgagcat tcaagatttt tagcaacaca agcaagagat caagcaccgt
     1801 attatcaaca tagcgaaata ggttataatt accgtctaag caatatttct gccggtatcg
     1861 gacgtggaca aatggaagta ttgaatgata gagtagctaa acgaagagaa attttcaatc
     1921 gatataagga agctttttct ggaattgata gaattgaaat gatgccagaa gtggatggca
     1981 tggtgccaaa tcgttggttg tctactttaa cgatcaatga ttataattct attgatcaag
     2041 tagttaacgt aataaataaa ctaaatgaaa aaaacattga aacaagagct ttgtggaaac
     2101 cactacattt gcaaccagta tttcaagggg taaaaatgta ttaccatgat aataatctta
     2161 gtgtgtcaga aaagttattt aatagcggct tatgcttacc ttctggaagt aatatgacag
     2221 atagtgaaca agaatttgtt attgatgagt ttaaaaaaat attcaagtag gtgtgatatt
     2281 ttatgaacat aggaattatt ggtttaggtt ccatggggaa aagaagactt agactcattc
     2341 aagaacactt taatgatatt ttactatgtg gtgttgatac taatgaggcg agacgaaagg
     2401 aagttgaaaa ggaatttgga ataccaactt ataatagtct aacagaggca aaaaatttgt
     2461 ttgaaataaa ttcagttttt gtttgtacat cccctataac ccatgaattt attataaaag
     2521 aggctttaaa tttagattgt aatatcttta cggaaatcaa tttattaaat gaatattatg
     2581 atgaaataat cgaagtagct aaaaagaaag atagattact ttatctatct tctacatttt
     2641 tgaagagaaa agaaattcaa tatattaagt ctaaagtgac gcataataaa aaaatttctt
     2701 atcaatacca tgtaggacag tatttacccg actggcatcc ttgggaagat tatcgcaatt
     2761 tttttgtttc aaacaaaaaa actaatgggt gtagagaaat attcgctatt gaattacctt
     2821 ggattataga tacatttggg aaagttaaat cgtttactgt aatgacccag aaattatcca
     2881 atcttgatat tgattattcg gacagtttca ctcttattat tgagcatgaa actggtatag
     2941 ttggaacttt aagtgtagat gttgtttcaa gagtagctaa aagaaattta actataattg
     3001 gtgaaaagat tttaatagaa tgggaaggta acccggattc tctattttca tggaatgaag
     3061 aaacttctag tatgacagat attcaattgt acgatgaggt tatgcatgat aataattatt
     3121 cacatactat tattgaagat gcatatttag aggagattaa agaatttttc acgcttttgg
     3181 aaaagaaaat ggaaccccaa tatacttttg aaaaagataa gtatattatt aacttaatta
     3241 acgacattga ggtaaattaa tgatgaaagt aatttttttt gggttagggt caattggaaa
     3301 gagacatcta aaaaatctaa taagctatgg aaaaaagaat tctattaatt ttgatataac
     3361 agcttatagt agcagtggaa aaaaagagtt tgaagaaata acgtatattc aaaatagaga
     3421 cgatttggat acacattatg atatagcttt tattacaaat ccgactttta agcatctgga
     3481 aacactaaat gtaattgttg aaaaagttga ttacatattt ttagaaaaa
//
LOCUS       DAYYHS010000165         3356 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000165 DAYYHS010000000
VERSION     DAYYHS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.172"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..493)
                     /gene="greA"
                     /locus_tag="ACHA6A_002943"
     CDS             complement(14..493)
                     /gene="greA"
                     /locus_tag="ACHA6A_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8971052.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(629..1804)
                     /gene="mltG"
                     /locus_tag="ACHA6A_002944"
     CDS             complement(629..1804)
                     /gene="mltG"
                     /locus_tag="ACHA6A_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8971053.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2008..3177
                     /locus_tag="ACHA6A_002945"
     CDS             2008..3177
                     /locus_tag="ACHA6A_002945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8971054.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         1043 a          649 c          529 g         1135 t
ORIGIN      
        1 tctttcagtc agactacgct tgttctactt tgacgatttt aactaacata tcgccacctg
       61 gagtagcaat agttacttca tcatttttac gtttgccaat caatgcttgc gcaataggag
      121 aatcattgga aattttgcca gaaaacggat ctgcttcagc gcttccgaca atcgtgtact
      181 cttcttcttc tccatctggt agttcgatga atgtaactgt tttaccaatt gatacctcat
      241 ctgcatctac accgtcgtta tcgatgattt gagcaaaacg aatcatattt tcaattgttg
      301 tgattcttcc ttcaacaaat gcttgttcat ctttcgctga ttcatattca gagttttcgg
      361 aaagatctcc gaaaccgcgg gcaattttga ttcgttcaat gatttctccg cgtttgactg
      421 tttttagttc ttctaattct ttttctaatt tttctttacc ttctagagtc attggataaa
      481 ctttctctac catgtgtaac gtctcctttt ttcgataaaa taccgcaaaa ggtaatcttt
      541 tgaggaatat agtccaaaaa agattaccac gcttttcttc aaatgtaaat gtttttctaa
      601 aaatgatcaa tgtctagagc ctgtttttct aagaattatt tacatatttt tccactaatt
      661 ccatatgttc ttcataagtt ttagcgaaat aaacatttcc agtgctcgta tctgctacga
      721 aataataata gtcattttct gctggttcta atactgcctt gattgcctct tcgcttggat
      781 tgtcaaaagg tccaggtcca tagccggtat tggtatacaa attatatggt gaatctacag
      841 ctgtatcttc atacgtcact aattctttgt gttcacctaa tgcgtataaa acagagatat
      901 ctgactgaag tggcatattt gctttgattc ggttaaagaa gacttgtgca atgtttttac
      961 gatcattttc ttttacgcct tctttttcaa ctagagaagc tagagttaaa acttcttgta
     1021 cagtcaaatt tttctgcttg atagctgaat aatagttttg catgaccgta ttcgtcttgt
     1081 tcaccatttc aatcactaga tccttaagcg aagtactcgt gtaataatca tatgtggctg
     1141 ggaataagta accttccaaa cggtattttg tgttctcagc tttagatgca ctttccaaca
     1201 gttctgggaa tttttggtgc aactcgttaa agaaagtctc atcattcact aaatctaaaa
     1261 attctttctt gtctttacca gttaccttcg ctacccgttc agcaatctga tcgatatcgt
     1321 acccttctgg aatggcaatt ttcccatctg caacttttgt tggctcagaa gtaccgcctt
     1381 cttgcaactg tttgccaatc tcgtcaagag tcatattagg cgctaactga taatagccag
     1441 cttgaaagcc agttaaattt ttaaattttg tataataatt gaaaacaatc ccgcttttaa
     1501 taatattgtc cttctccaaa atctcaccaa tctgcttgtt agaagagcca ctcgggatct
     1561 cgacttggac taattggtca tctgatttat ctaaaggctt caacccgcta tctacataac
     1621 gatacacggt aaatccaaga aatccgccaa taatcaataa tgtaagagca atcactaaaa
     1681 tgatcttcct cacaatctta tcttcttttt tacgcagatt tctccgcttg tcgattatat
     1741 ctgctgtagc tgccttactg gcttcttttt tatctttact tgggtgttgt ttgtttcctg
     1801 acaaaacaac tcctccttac tatcaagttc gtaactttat tcattataca tgatatttga
     1861 attggcgtga atgattttat ttaaatattc tatcaaaaat gtaagtagta aatccattac
     1921 tataagatta aaataaatgt tatcaagata caagctattt tagctttatt ctttctgtaa
     1981 aaaagaaagg agactaaata aactattatg aaaaaaggaa aacatgctaa aaaaagaaaa
     2041 tattcatttt taatgatcgt tgtcgtgttg tttagcctta gtgcgttctc tacaggaact
     2101 attcttggat ggcaatttta caataatgaa cactctcaga tagttgccca tgctcaatca
     2161 gctcaaaaaa agttgatact gactaccaat gaacaacagc tcgttgaaaa aatcgatcaa
     2221 ctattaagaa aaaatgatta tattggctca atttacgtaa agaaaaataa tcgcatcatc
     2281 atggaaaaag gctatggcta tgcgaataaa ctcacgcaac aaccaaatga tccttctctt
     2341 tattatcaga ttggttcggt tcaaaaagca ttgactactc ttttgatttt aaaacaagtc
     2401 gagcgtgggc acctttcttt agatactaag ctggctcaat tctatccaca aattgctggc
     2461 agccagcaaa ttactatccg tgacttgctg tatatgcgct caggcttgca ccgaactgct
     2521 tctccaacca taccgatgac tgatgaagaa gtcgttcaat ttgcgattca gcacttaaaa
     2581 tttacagatt atcagacctc tcattatgat tcattgaact ttaccatatt gactggaatt
     2641 ttgatcaaat taacaaatca accgtatgaa aaattattga ccgatgatat catcgaacct
     2701 ttaggattac agcatacaaa attttatgac caagtcaaaa attcatccaa tcatgcacta
     2761 tcctatcaaa tgtctaaaga ggatgattac tttaaaccac tgacagaatc tgaaacagag
     2821 atccataacg aattagggac tggtaatatc agtatgtccg tctatgattt aaatacgctg
     2881 tttacacaag ttttatctgg aaaactagtt tccaaagact tactcttctc attatggcgt
     2941 gaaaatgcga atggacatcc atatagtggt ggggtatata gcggagataa ttatatcctg
     3001 tcgcaaggaa atatcgactc ttttgataca tctgctatat ttagcaaaga ttcacaggat
     3061 gctatcatca tggaaagcaa tataaaagct gacaaaacta tcaaactgcc tactgctgat
     3121 ctaagaaacc aaatctatga cctgatggaa ggaactacta agttggcaaa tagctgatat
     3181 gatgaaaaga tagcaaatac tacttctttg aatagcattt gcttaaaaaa agtactcgac
     3241 taaacgtagg aaagagtgtg ggacaaatct aaaaatcaat ttttgctaca gactttaatt
     3301 ccaaataaac agaacagaaa gaggtgttct tctttctgtt ctgtccaaat tattct
//
LOCUS       DAYYHS010000166         3210 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000166 DAYYHS010000000
VERSION     DAYYHS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.173"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACHA6A_002946"
     CDS             complement(139..1338)
                     /locus_tag="ACHA6A_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8971055.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACHA6A_002947"
     CDS             complement(1468..2844)
                     /locus_tag="ACHA6A_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8971056.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..>3210)
                     /locus_tag="ACHA6A_002948"
     CDS             complement(2844..>3210)
                     /locus_tag="ACHA6A_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8971057.1"
                     /translation="IENIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIA
                     RALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADII
                     LELSGKKLKKVNKMNLEVE"
BASE COUNT          982 a          613 c          454 g         1161 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt
//
LOCUS       DAYYHS010000167         3191 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000167 DAYYHS010000000
VERSION     DAYYHS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.174"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(551..2164)
                     /locus_tag="ACHA6A_002949"
     CDS             complement(551..2164)
                     /locus_tag="ACHA6A_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8971058.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..>3191)
                     /locus_tag="ACHA6A_002950"
     CDS             complement(2305..>3191)
                     /locus_tag="ACHA6A_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8971059.1"
                     /translation="QSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANLYAEFAHKS
                     IELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSI
                     ITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSL
                     PRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFF
                     ISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSS
                     AFKKYTGLSPSKYYLSLK"
BASE COUNT         1029 a          548 c          568 g         1046 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg t
//
LOCUS       DAYYHS010000168         3162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000168 DAYYHS010000000
VERSION     DAYYHS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.175"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHA6A_002951"
     CDS             complement(63..2555)
                     /locus_tag="ACHA6A_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8971060.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHA6A_002952"
     CDS             complement(2562..3029)
                     /locus_tag="ACHA6A_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8971061.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAYYHS010000169         3048 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000169 DAYYHS010000000
VERSION     DAYYHS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.176"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1801
                     /gene="alsS"
                     /locus_tag="ACHA6A_002953"
     CDS             137..1801
                     /gene="alsS"
                     /locus_tag="ACHA6A_002953"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8971062.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1814..2524
                     /gene="budA"
                     /locus_tag="ACHA6A_002954"
     CDS             1814..2524
                     /gene="budA"
                     /locus_tag="ACHA6A_002954"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8971063.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2654..>3048
                     /locus_tag="ACHA6A_002955"
     CDS             2654..>3048
                     /locus_tag="ACHA6A_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8971064.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGV"
BASE COUNT         1019 a          510 c          686 g          833 t
ORIGIN      
        1 ttttgaaaca tatggctaaa caaatgaaat aaaacatgct atacttttga atgaaacagg
       61 tttagaaact gtatcactta tttgtgatta tttctaacta ataattaaaa ataaagcata
      121 aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt tcgaaatctg tagtagaaag
      181 tttgattaat catcatgttg attatgtatt cggtatacca ggtgcaaaaa tcgatggtgt
      241 atttaatgaa ttagaggatc aaggtcctga attaatcgtg actcgtcacg aacaaaatgc
      301 tgcatttatg gcacaagcaa tcggacgtat tacaggagag ccaggagtag ttatcgcaac
      361 aagtggtcct ggtgctagta acttagctac aggtttggta acagcgacag cagaaggtga
      421 cccagtttta gcaattgccg gacaagtaaa acgaagcgat ctattgaagt tgacccatca
      481 aagcatggat aatgcagcac tgttccagcc aatcacaaaa tacagtgcag aaatccaaga
      541 tccagaaaca atttcagaag tgattgccaa tgcttatcgt atggctaaga gttcaaaaaa
      601 aggcgcgagc tttatcagta tcccgcaaga tgtagtggat gcacctgtcc aaggtaatgt
      661 catcaagcca ttgagcgatc ctaaattagg ttctgcttct gcagatgata tccgttacct
      721 agctgaacgc attagagaag caaaactacc ggtactatta gtaggtatgc gaggatcaag
      781 tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag acagcattgc ctgttgtaga
      841 gacattccaa gcagctggag tgatctcacg tgaattagaa gcgcatttct ttggtcgtgt
      901 gggtttattt agaaaccaac caggagatat gcttttgaaa cgaagtgatc tagttattgc
      961 aattggctat gatccaatcg aatatgaagc aagaaactgg aatgcggaaa aagatgcgcg
     1021 gatcattgtg atcgatgaag cgccagctga aatcgatcct tttatgcagc ctgaaagaga
     1081 attgatcggt gatatctcag ccactcttga tttattgaca ggttcattag agcctcaaca
     1141 agtgtcagaa gatgcaaaag aatatttagc atctcttcaa gcaaaattaa cggagcggga
     1201 cattgttcaa tcaaaaggag aagcaggtat cttgcatcct ttagaagtta ttaacacgct
     1261 tcagtcaaaa gtaacagatg atatgactgt taccgtagat gtaggtagtc attatatttg
     1321 gatggcacgc cactttagaa gttatgaacc tcgtcatttg ttattcagta atggtatgca
     1381 aacgcttggt gttgcattgc catgggcgat ttctgctgca ttggtgcgtc caaatacaca
     1441 gatcgtatca gtttccggcg atgggggatt tttattctct gcccaggatt tagaaacggc
     1501 ggtacgtaaa aaattgaata tcgttcattt gatttggaat gatggacact acaatatggt
     1561 agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt gtagactttg gtccagttga
     1621 ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc cgagctacaa gtgtagagga
     1681 attagaaaaa gctttagaag aaggatttgc gacagaagga cccgtaatta ttgatattcc
     1741 gattgattac cgtgataatg agaaattggg agaaacaatc ttaccagatc aattctatta
     1801 aggaagtgtc gacatgactg aaaaaatctt ataccagcac gggacattag gagctttgat
     1861 ggccggcttg atggatggaa cagaaacgat tgctcatata ttagaaaaag gaactctggg
     1921 attgggaaca ttgcatgggc ttgatggtga agtcatcttt ttagatggcg tagcttatca
     1981 aggaagatca gatggctcag ttgcccaatt agatggaagt gaacttaccc cctatgcagc
     2041 aattacagat tttacgccag atactagttt ttctgtctca aaaactgcag atggagaaca
     2101 attgaaaaaa gacatattag taagagaagc cggggaaaac ctatttttgg cagtgaagat
     2161 aactggatta ttcaaaaata tgcacatccg tatcatgccg aagcaagaaa aaccttacag
     2221 aagactagca aaaatttcag agtctcaacc agaattccaa caaagcaatg tccaaggaac
     2281 attagtcggg ttctttacgc ctgaattatt ccaaggagtt gctgcagcgg gatttcacct
     2341 gcattttatc gatgatacac atacatttgg cggacatgta atggattttg aagtggctga
     2401 aggaaaagtt gagattagcc gcatctcgtc ccttgtacag cattttccag ttgatgatcc
     2461 gacttttgtt cacgaaacga ttgattatgc tgatttagca gcagaaatcc aacaagcaga
     2521 atagaaaaag acagcgagat gcgttcaagg tgattaacct gacgtatctc gtttttttga
     2581 ggagaaaacc tagcgtttac aaaaaaactg agctataatc ttctccatag atcaaaaagg
     2641 agcggttttt atcatgtgga cagaaaaaca gaaaaaagat gcatacgaaa ttttattatt
     2701 acagcaacaa ggattattag aagcagaaga taactggtta gcaaatttag caaattcatc
     2761 tgcattgttg aatgagactt tgcctgagac tgtctttgca ggatattatt tattcgatgg
     2821 taatgaattg attttaggac cgttccaagg gcgagtttct tgcacacgta tcaagatggg
     2881 aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca ttgatcgttg atgatgtcaa
     2941 aacccatgca aattacattt cctgcgattc agccgcacaa tcagaaatcg tagtgccaat
     3001 ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat tgcggagt
//
LOCUS       DAYYHS010000170         2831 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000170 DAYYHS010000000
VERSION     DAYYHS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.177"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..191
                     /locus_tag="ACHA6A_002956"
     CDS             <1..191
                     /locus_tag="ACHA6A_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM8971065.1"
                     /translation="NKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTSLLIIS
                     LVIYFLKIKNRIIDDYNS"
     gene            204..1211
                     /locus_tag="ACHA6A_002957"
     CDS             204..1211
                     /locus_tag="ACHA6A_002957"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8971066.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1223..2227
                     /gene="neuB"
                     /locus_tag="ACHA6A_002958"
     CDS             1223..2227
                     /gene="neuB"
                     /locus_tag="ACHA6A_002958"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8971067.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2224..>2831
                     /gene="neuC"
                     /locus_tag="ACHA6A_002959"
     CDS             2224..>2831
                     /gene="neuC"
                     /locus_tag="ACHA6A_002959"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8971068.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKK"
BASE COUNT         1049 a          354 c          542 g          886 t
ORIGIN      
        1 cgaataaaca attatttgac acaaaaaaaa tatgtatcat aataattttt gtatcaattt
       61 tttctttgat acttcatatt ttaatcaatc aaatgttgat tcgttacgtt ttgacatcat
      121 tattgataat tagtttagtt atatattttt taaagattaa gaatcgaatt atagatgact
      181 ataattcata ggaggagata taagtgttta aaggaaaaac aatattagta actggcggaa
      241 ctggttccat tggaagtgaa attgttcgtc agcttttaaa atatgaacca aaagctatca
      301 gaatttatag tagaggtgaa gaaaaacaat tttatatgca acaagagctc tcaaatcaca
      361 ctaatttgag atttttaatt ggtgatatca gagactctca aaggattgat tatgcatgtc
      421 gtgatgtgga tattattttt catgctgccg caatgaaaca cgttccagca agtgaatata
      481 atcctatgga agctgtaaaa acaaatgtga tgggaactca aaatgtaata gatgcagcga
      541 tcaaaaataa tgtaaaacat tttgtaggta tcagtacaga taaagtagtg agtcctacta
      601 acaccatggg tgcgacaaag ttattgagtg aaaaattaat aatagctgcc aatcagtata
      661 aaggaaaaac agaaacaata ttttcttgtg taaggtttgg aaatgtcatg gggtcaagtg
      721 gatctgttat tcctcttttt agtaaacaaa taaaaaacaa taaagatatt actttgacag
      781 atgttgatat gacacgattt atgatgtcaa taccggatgc tgcaagatta gttctgtctg
      841 ctgctatgaa ttcaaaattt ggagaaacat atgtcttaaa aatgccggca atcaatattt
      901 taactttagc tcaatcatta ataaaaatat ataatgatac acatgatgaa aaatatagag
      961 gtgaaataaa agttgtaggt atcaggccag gagaaaaatt atatgaagag ctaatgacta
     1021 cagacgaaat tgaacgctca tatgaaaatg ataaaatgta tatcattcca ccacactatc
     1081 atgctactga cgaactgtac aaagattatg aaaaaattaa aatcactgca tactcttcag
     1141 atgcaacaga attaatgagt ggacaggaat ttattagaat cgcaaaagag aataatgtcg
     1201 tgacttggta aggagtttta tgatgaaatc agaagtatat gtaattgcag aaattggtgt
     1261 aaaccataat ggaagtctag atttagcttt agaaagtatt gataaagcta aagaatgtgg
     1321 agctaacgcg gttaaatttc aaacatttaa aacgaataag ctagtttcca aaaaggctcc
     1381 aatggcaaaa tatcagaagg ataatcttaa agaggacagt tctcaatttg aaatgctaaa
     1441 aaaattagaa ttatctcaat ctgattttat tagaataaaa gattattgta tacaaaataa
     1501 aattgatttt ttgtctactc cttttgatga agaaagtgca gactttctaa aacaaattgg
     1561 tgtacaagga tttaaaattg gctcaggaga tttaacaaat ttaccttttc ttagaaaaat
     1621 agatatgtat gggttgccag ttttattatc tacaggtatg gctgatattc atgaggtcag
     1681 tgaggctgta gattgtttca tagatagtcc agttactatt ttacattgta cttcaaatta
     1741 tccggctagt tctgaagata ttaatttatt ggcgattaac acattaaaag aaatgttcgg
     1801 gatacccgtt gggtactccg atcattcatt gggatatgat gttggtattt gtgctgttgc
     1861 gatgggtgct aaggtaattg agaaacattt taccttggat aaggaactac ctggtccaga
     1921 tcataaagct tctttagatc ctcaggaatt tgcagatttt gtaaagcata ttagaaacac
     1981 tgaaatattt ttaggtgatg gtgttaagcg agcaatgcct tctgaactta gtacaaaaga
     2041 agtatcaaga aaaagcctag tggtcactga gaatttaaat gtgggagata ttcttacaga
     2101 gaaatctctt acaataaaac gtcctggaaa tggaatagct cctaagtttt tatatcaata
     2161 tattggcaaa aaagttaaaa gaccaataag tgcagatagt gttctgcttg aggaagatgt
     2221 tgaatgaaaa aaatacttat agtaagtgga acacgagctg attatggaat atataaacct
     2281 gttgctgaga atttactaca agataattgg gatgtatcat ttgttgtttc tggaatgcat
     2341 ttatcccata aatatggatt tactaaagaa ttgatcttag ctgacggatt tagaattagt
     2401 ggagaagttc aaagtttgtt tttagagaac aataagggaa acatggctcg gtctgtggca
     2461 ttagagataa atggtttcac aaatatattt gaacaaacta gccctgattt tgtattatta
     2521 ctaggggata gaggagaaat gttggcagct gcgatagctt gtttgtatct tggaatcaaa
     2581 acagcacata ttcacggagg cgaagtgtct ggtactgtag atgaatcaat aagacatgcc
     2641 ataacgaaaa tagcatctgt tcattttgct gctacggaaa aaagcaaaga gcgtttaata
     2701 aaaatggggg aagattcttg gagagttttt cccgtcggag ctcctagaat agatactatt
     2761 ttaaataata gattacctag ttttagagat ataaaagaaa aatatcatct aaaatttgaa
     2821 aagaaaaaat a
//
LOCUS       DAYYHS010000171         2773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000171 DAYYHS010000000
VERSION     DAYYHS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.178"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA6A_002960"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA6A_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8971069.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            661..915
                     /locus_tag="ACHA6A_002961"
     CDS             661..915
                     /locus_tag="ACHA6A_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8971070.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            916..1233
                     /locus_tag="ACHA6A_002962"
     CDS             916..1233
                     /locus_tag="ACHA6A_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8971071.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1792..2727
                     /locus_tag="ACHA6A_002963"
     CDS             1792..2727
                     /locus_tag="ACHA6A_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971072.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          985 a          394 c          514 g          880 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatg gatgttaacg
      481 gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt
      541 ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg
      601 gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga gtgtgataac
      661 atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc
      721 tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa cttattcata
      781 ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa
      841 gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac
      901 ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt tcagaccagc
      961 cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa
     1021 agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat
     1081 gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa
     1141 agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt aggacgtatt
     1201 ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag tcgctcatcc
     1261 tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt actcgaagat
     1321 tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa ggtctaaaga
     1381 tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat cgcttggtga
     1441 ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat tcccaaaaat
     1501 ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat tgacgtaact
     1561 tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa gaggaagtcc
     1621 aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat acatccctgc
     1681 tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa tgggataaaa
     1741 tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat agtgaacata
     1801 cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac tttcaaaaga
     1861 ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata caagtttaat
     1921 tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc tgagagtagg
     1981 ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat atatacgttc
     2041 cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga atatctcaag
     2101 gggaatagct atctcaagat tcgattttca catatattaa ttttactacc atatttgaat
     2161 gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa tcaaggaaat
     2221 atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg ttacggaatt
     2281 ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg ttcattatta
     2341 atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga aaacatgcgt
     2401 ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt tttgaaaatt
     2461 caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc acattttggt
     2521 gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa aatcaatgct
     2581 ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat aacagatatc
     2641 tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact tgttaataaa
     2701 aataaaggaa cccgtggtgc tagttaattt tttcctagag aaaaatgtct aaacatgttg
     2761 gtaaatcaac aaa
//
LOCUS       DAYYHS010000172         2700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000172 DAYYHS010000000
VERSION     DAYYHS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.179"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            724..810
                     /locus_tag="ACHA6A_002964"
     CDS             724..810
                     /locus_tag="ACHA6A_002964"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8971073.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1112..1336)
                     /locus_tag="ACHA6A_002965"
     CDS             complement(1112..1336)
                     /locus_tag="ACHA6A_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971074.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1702..1986
                     /locus_tag="ACHA6A_002966"
     CDS             1702..1986
                     /locus_tag="ACHA6A_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971075.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1989..2405
                     /locus_tag="ACHA6A_002967"
     CDS             1989..2405
                     /locus_tag="ACHA6A_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971076.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2542..>2700)
                     /locus_tag="ACHA6A_002968"
     CDS             complement(2542..>2700)
                     /locus_tag="ACHA6A_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8971077.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          891 a          500 c          434 g          875 t
ORIGIN      
        1 ttccgttttt tcatccattc ctttcttccc cccttacaaa taaataataa cctttaacaa
       61 ccaactcgta aataggttgg ttgttattat acaaaaggat attagtactc agataaaaag
      121 ttgttagtac tcagataaaa agttgttagt actcagataa aaagttgtta gtactcagat
      181 aaaaagttgt tagtactcag ataaaaagtt gttagtactc agataaaaag ttgttagtac
      241 ccttctgtat ccgttgggag agtaaggctc aactgccccc taaaaagggt tctaaaaaat
      301 ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa aatttcccgc caaaaaatca
      361 cccacttttt aaaataaaca ggttcgcgca tgatattaaa ctgccaatct agctccctat
      421 ggtcgctgta agaaggagtg atcgctacgc tctcacattt tacttgcagc agtcgccttt
      481 aggctccttc gccaaaaata attcattagg gactcctttg tggcgtcctg agagctcgtc
      541 taagccgttc taagacaaga ttcgataaaa actatgtcct tcgttctaaa gcgccttaca
      601 ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa aacttaggct tatttttgct
      661 tttttattgt ataattgatt gtcgcctatc cgacagtctt gcgactggaa ggaggtgtgt
      721 ttcgtgttca ctaactttat ttgctccctt cttgttgggc ttttaagcgc tttgttcagt
      781 tactggttga ataaccgaaa caagaagtaa aacttaatta aggcgactca gcccacaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgtg tttcgtgttc acttatttgc
      901 aatcaaatta taacagaaat aaatatttat ttcaaaaata tcctacaaaa gtctaaaatc
      961 cgtaattatt tcttatatgc aacatttaca aataaagtcg ctctgagagc tcgtattttt
     1021 cgatttaaga cgttttattt agtttttggt gtatttcatt ggtccaagct aaaaaactct
     1081 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
     1141 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
     1201 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     1261 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     1321 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     1381 ttctttgccg tctatactct caaaaaaaat gtcgtttgaa aaactaaata aatttttgat
     1441 cgtgcatttt taaatttggt tatcgattac acgtgcagca ctcccccccg aattttaaag
     1501 agcataagga aacgtgtacc cactttctaa aaaaagtggg tagataggct gtgcctatca
     1561 cgtttctcag aaaaaaattt gatatcactt tttcgccatc gtgggtagct tatgtatagc
     1621 attttggcaa aaaacagcta tacaaatata gtatttgtat ggtatacata aattatacat
     1681 aattgcaaaa ggagattttt tatggacatt ttagttcgga atattgatcc aaagtttgtt
     1741 gcaacaatag acaaacgttg ccaagaactc acgaataaaa caggagaaaa atggagtcga
     1801 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
     1861 aaagaccaat tcgatttatt gctcgataaa tttattgcta tccaaacaga aaatactcaa
     1921 atattaagcg agtatgtcta cacaaataat caaatcattg aaatgcttat cggaggaatg
     1981 atataaccat gagaaaaact tctgtccaaa aacatgttac tttgtaccct agtcatgttg
     2041 acgttttaga ttccatcatc gaaaaaaatc cagatatcaa taactactcg gaagccattc
     2101 gctttttatg tatgagttat gaacccgata ccaacacgac cactaccagc aatcaggcaa
     2161 aactaaatgc tatgggaaaa gaaatttcca tcatgactga aatcatcgca gaatttgctg
     2221 atttatatct aaaagataca ggcgttctta ttggcacaaa ttctaaagta tatcaagaag
     2281 tgaaagaaaa aatcgaagct aaactaaatc catcaaatcg aaatcgacca aagaatcatc
     2341 aaaaagaaac cagtcaaaca gtcacttcta gaaatttcga cccaacaaat tttctaaaac
     2401 gataaaaaac agataggagt tattcctatc tgttttttat ttagaagccc catacaatat
     2461 gctagaatgt actagcaatc ttagtcggtt gtaagggact tgtctacttg cttaatgtaa
     2521 ggggggcgag ttctttttta tttattcttc taaccagtta cgcagagaaa tctttggcaa
     2581 atctgacgtt aggcttgaaa aagtaaattc taatttggcg attttattgc ccttttttgc
     2641 ttttattttt gtgactttaa gatcattgaa gtattttttt agttctcgta atgcaggatt
//
LOCUS       DAYYHS010000173         2683 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000173 DAYYHS010000000
VERSION     DAYYHS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.180"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..1496)
                     /gene="menA"
                     /locus_tag="ACHA6A_002969"
     CDS             complement(543..1496)
                     /gene="menA"
                     /locus_tag="ACHA6A_002969"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8971078.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1509..2612)
                     /locus_tag="ACHA6A_002970"
     CDS             complement(1509..2612)
                     /locus_tag="ACHA6A_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8971079.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
BASE COUNT          833 a          561 c          470 g          819 t
ORIGIN      
        1 aaaaagtgtg actttgtata aataaattag aagaagcaaa cgaggatgtt aaaagaatac
       61 catatagtta ccaaaccaag aaagttctcg tcaagttcgc tggtacgaat caaatcaagt
      121 gaaaaaaggg actgtgaaaa aaattgtgtc atagttagtc actttttccg aacaaatagt
      181 gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
      241 tccatttgtt tctctaatac tcaggacaaa acactgattt tacaacctct gataaacggt
      301 gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg aaagccattt
      361 tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc ctgataaacg
      421 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
      481 tttttatttg gctgttctta ttacttgatg cagggcagtt ttttcacaac ctcttcatgc
      541 tgttaaatgg cttgcataat gattgttagt aatagcccca atacataact ggcattgaat
      601 aagaccatat ttttgatgga atagctgaaa ctgataggct ggggtaacgt ttgcttgaat
      661 tgatccaaat gcttcttcac aattggcaag gtcaagaagg tgaccaagat cggccattca
      721 tagataccgc tcaacaaccc cattaaaatc acgaaatagc aagcatacat caatagctgg
      781 aataagactg ttcccgcagg acgtccgata taaaaaacta gtgtatagcg atgattttca
      841 atatcacggt caagatcccg caaattatta gccaacatga tattggcaat cgtaaacacc
      901 aagggtaagg aagctaatac gattgctgct atagcccaaa gatttcctac caaagcaaat
      961 tgtccagatg aaaagttgac tgtcagatca tagacatctg aaccgaatgt attgacataa
     1021 acgaccatgg caaaaattcc aaggcccata gtcaccccac tgaaaatttc ccctaaaggc
     1081 attcgagata aaggaatcgg tccgaatgta tagaatactc caataaaaca acagataaat
     1141 cccatcaaaa gtaaaagcca acccgtattg atcgttagaa taacgccgat aacaaaaaca
     1201 aatgcaatca tagctaaaat catgtttcga atcaatacag gagatagatt tctgcggcca
     1261 atgacatttt cttcatactt atatacttca gaatgagcct tttcataatc catgtaatta
     1321 ttgatagcgg tagtcgctaa atcaaaaact agcattccaa taaaaaataa aaaagtgttt
     1381 agccaatgta tctcatgaaa aaaagcgata gagaataata cccctaccgc aaaaggaaat
     1441 acactagcca cctttgttcg tatttcaact acttctaaaa agatttttag tgtcatggtt
     1501 cgtactcctt attttagttc agaacggtca cccatcgtgt aatgggagtc ctccgataat
     1561 ttgaaggtgt ctttgattgg atcggtcaca tatactttat cgtcttttgt cacgaagatc
     1621 gcttccgtcc cgttattcaa ttcgctttcg acgtattcca gtcctttttt cacacccatt
     1681 gaaaagactg ctgtagacaa cccgtcacca tcaattgatt tatctgttat gatcgtgaca
     1741 cttgcgatat cattgtcgaa cggataaccc gtttttggat caaacaaatg atggtatttt
     1801 ttgccatcta cttctaaata acgctcgtaa attccagagg taactaatgt tttgttccgt
     1861 tctttaatag aacctaaaac agatccgcga gccatgttcg gatcttggat gccgactgtc
     1921 cagtcttggt tttcgcctct tgggctgtgg cctaagacat agacattacc gcccaagtca
     1981 acgatggcag tggtcactcc ttgttttttc aagactttca ctacttcgtc cgtaatgtaa
     2041 cctttggcga ttgcacctaa atcgatgatc attccttttt cttttagata aacagttttt
     2101 tctttgtcat ttagttcgat cttatgataa tccactaatt tcaatgcctg atcgatttct
     2161 tcttgggaag gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt gattgcacca
     2221 atcgccatat cgaatccgcc ttgagaatct tggctatatt cgtaggcccg cttgactaac
     2281 gtgtagacgt catcagaaac ttttactggt ttgatcccag cttgctcatt cacttcatcg
     2341 atctcagaac ctttttgatt gatcgtgatt ttatcaccaa gttcttttac tcgatcgaag
     2401 gcaggtttca atgctgattc tttgccttca tcatacacac ggatacgaac gtaagtccct
     2461 aacaagaact gttcatcaga atatggatct ttcaatatct tggtagctgc tgaactttca
     2521 gtggttgttt tatttgctga gttgctgcag ccagttagca ccataaaaga gatgcccagt
     2581 gtgattaagg aaaatagtag ttttttccgc atctttttcc tctcttcatt ttgaaaggtg
     2641 agacgactgc ctaatctggc tatcgtccca ccctccaact ttt
//
LOCUS       DAYYHS010000174         2673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000174 DAYYHS010000000
VERSION     DAYYHS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.181"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(194..1585)
                     /locus_tag="ACHA6A_002971"
     CDS             complement(194..1585)
                     /locus_tag="ACHA6A_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8971080.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1798..2184)
                     /locus_tag="ACHA6A_002972"
     CDS             complement(1798..2184)
                     /locus_tag="ACHA6A_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8971081.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2240..>2673)
                     /locus_tag="ACHA6A_002973"
     CDS             complement(2240..>2673)
                     /locus_tag="ACHA6A_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8971082.1"
                     /translation="LFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTHRLTEST
                     WGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDRRLDIEA
                     GKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
BASE COUNT          798 a          582 c          460 g          833 t
ORIGIN      
        1 atgaggaact atttttgttt ggctgttctt attacttgat gcagggcagc tttttcacaa
       61 cctcttattc acggtgtttc agaagtagct tctcggaaat aagccgaaat ttcacaaaaa
      121 tttgagaaac tatttttagg aaacttcttc ttatttctta aagctgacca cttctatcac
      181 aacctctttt tgactatgca ttcacatcga tcactttaga atcatcattg atgacatcgc
      241 gatctaaatc tttgatgcgt gcacttgtca cgataccagc taccatgcta ccgctgacgt
      301 ttactgctgt acgtgccata tcgattaatg gttcaacaga gataactaac ccaacgattg
      361 ctactggcaa gttcattgcc cctaaaacga tcagtgaagc aaatgttgcg cccccaccga
      421 ctccagctac accaaaagaa ctgattgtta caattgccac aagcatcaaa ataaattcca
      481 aactaaagac attcactcca gctgctggtg cgacgatcgt tgccaacata gctggataaa
      541 cgccagcaca gccgttttgt ccaatagaca aaccaaaact tgctgagaaa ttagctgttg
      601 catcatctac tcctaaagct tttgtctgag tctcgatatt caatggcaaa gctccagcac
      661 ttgaacgtga agtaaacgca aagctcaata ccggaaaggc ttttttgaaa tagttgattg
      721 gattgacttt tgcaccaatc aaaatcagtg agtggacaat caaaacagca agcaatgcta
      781 cgtaagaagc aacgacaaat ttacctaaat tgacgatagc tgcaaaatca cttgttgcca
      841 aaacgtttgt catcaaagca aatactccat aaggtgtcaa acgcaaaaca agcgtcacga
      901 tccgcataat gattttatac agactgtcga ctaaatctga aaagaatgct gcttcttttg
      961 gcgcttttct tttcacgcct agataagcga ttccgacaaa tgccgcgaaa ataacgactc
     1021 caatcgtact tgttgcacga ctacctgcta aatcagcaaa aacgttttta gggataaagg
     1081 ataaaatctg ctgtggaatc gataagtttt caactgtttc ctgacgtgtt gccagttcag
     1141 agattcgtgc tgtttctgca gctccttgtg tgaatttagc tccttggagt ccgaacagca
     1201 ttgttgaacc aattccgatc aatgctgcaa tagctgttgt tccaagtaaa gaagtcaata
     1261 cagtcgcact aattttccct agcttttcag aaactttcat ttttgtgaat gcaccaatga
     1321 tcgaaataaa cactaaaggc ataactagca tttgcaagaa tgatacatat ccttgaccta
     1381 caatcccgat ccattccatt gattgagtaa cgactttgtc gtctacacca aagatcaatt
     1441 gaagcagacc gccaaagacg ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt
     1501 ttgtatggcg cttttgcata cgatagaaca catataataa tgccgcaaaa actagaacca
     1561 ctaaaactgt gatgagtgtt gtcatttttc tcctcctcat attattaatg acaagtccat
     1621 cgaaggaagt aaaggttccc gagaatgatt cttcaaccat cctcctgaaa aaattttttt
     1681 cagtgacttt tatattttag acgattttta tcgaaatgac attctttttg cttacaatta
     1741 gtaataaaaa aacatacctg tgatagcttt aatctatcgc aggtatgttt ttactgtcta
     1801 ttcttgtttt tctgattgaa aattcaattt taccccaggc tgatctaagg cgatctctgt
     1861 cacctcataa gacattcggc gtacgatatc gaataacggt gctactagct cgtccacttc
     1921 ttcttgtgtg acatcatttt gaccttcaat tttccgattg aggatatgat tgatctgact
     1981 cacacttcca gataaaacat atgtatcaaa cactaaacgg aactctaagc gtgctcctac
     2041 gatgctatgt tctttcggat agttttcgat cgtttctttt aaaggcgaga agcctacttt
     2101 caaatctgtt tctacttctt ggttcggcaa tctcatatca taatgaaatg cttctacaac
     2161 ttctttttgt cttctgattt ccatataatt ttatcactcc tttatgaaaa ttataacata
     2221 tctcctttga aaggggatac tactttttaa tgatgctctg caaggtaagg atttcattca
     2281 gatttcctac cacataatct gccgggatct cacccagaaa atgatcgatg tcgtaaagac
     2341 aagtagcgac tcctgcattt ttccctgctt cgatatctaa acgacgatcg ccgatcatca
     2401 tcgtgtctgt tctttctaaa tgaaaagtat cgatcaaata atttaaagat gctggatcag
     2461 gtttacgagg aaagtcctga tcgatcccta ctacttcctc aatcagatgt gccaatcgat
     2521 gttcttttaa caggccccat gttgattctg tcaaacggtg ggtcaggatg aaatgacgac
     2581 cgccgttgtc ttttagcatt tccagtactt gtttcgtttc tggaaatgga cgggaaactt
     2641 ccgtactttc tttttcatat cggtgaaata att
//
LOCUS       DAYYHS010000175         2656 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000175 DAYYHS010000000
VERSION     DAYYHS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.182"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(184..750)
                     /locus_tag="ACHA6A_002974"
     CDS             complement(184..750)
                     /locus_tag="ACHA6A_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8971083.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(750..1706)
                     /locus_tag="ACHA6A_002975"
     CDS             complement(750..1706)
                     /locus_tag="ACHA6A_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8971084.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1706..2362)
                     /locus_tag="ACHA6A_002976"
     CDS             complement(1706..2362)
                     /locus_tag="ACHA6A_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8971085.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          825 a          543 c          488 g          800 t
ORIGIN      
        1 ctgcatcaag taataagaac agccaatcaa aaacagcttc tcatgttttt gatttggttg
       61 gacttattac cgcagatata agcttttgag caccgtgaat aaagaggtta tgaaatcaac
      121 gctctaacct gagtattaga gaaaaaaacg caaaacatat catttatgtt ttgcgttttt
      181 tatttatctt tttttctcaa tacaatataa aataggcggc tggttctttt ggttgataaa
      241 ccgataattc agcacattat atgtttcctg tggcagtttg gaacaaaaag aatcaaccat
      301 atccagttct ttttctccac cttcatgacc atagtagacg acaaggatca atcgtccgcg
      361 aggcacaagt cgtttcaata tttcttccat agctgtgcgt gtagtctctg gcatagtgat
      421 gattgcttta tcactttttg ggagataccc tagattgaaa atccctgctt tgagattttg
      481 ttgttcagct atcacttctc ctaacgtttc gtggccttgt agaaacaatg agacacgttc
      541 ttctaactga cgctgttcta gcctttcacg agtagcatcg attgcttgtt gctggatatc
      601 aaaactatag acatgaccag tttttccgat gtgttctgct agaaaaaccg tatcgtggcc
      661 attgcccatc gttgcatcta cgacgtgatc ccctggttgc aagatttctt gcagtaatgt
      721 atggctgaaa tgcaaagcag tttgcaacat cagacgctca cctttttccg tacatcatat
      781 tttccttgga agctatctct gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat
      841 tttttcaagc tccacattgg tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga
      901 tggatgatga tctcttttgg tatcatttcc agctggtcgc aaatgacatt gacatagtca
      961 ggacggctca ttaattgaag gcgaccttca tgatagtccc gcaacattct cgttttgcgc
     1021 atcagatgca tcaaatggag cttgattcct tggatatctg aatctaaaat ggtgcgtctg
     1081 acattttctt tcatcatttc taaactttcg cctggtaatc cgttgatcaa atgcgtacaa
     1141 acacggatat tatgtttgcg taatttagcc acgccatcta aataagtttg ataatcatgt
     1201 gctcggttga tcagttttga agtttcttca aaagttgttt gcaatccaag ttctacccat
     1261 aaatacatcc gctcgttcag ttcagcaagg taatcaacta cttcatctgg cagacaatcc
     1321 ggtcgtgtac cgactgataa tcctacgaca cctggtaaat tgactacttg ctcaaatcgt
     1381 tctttgatta tttcaactgg cgcatgggtg tttgtaaaat tttggaaata aacgatgtat
     1441 tgttggacat gaggccattt ttgatgcatc atgtcgattt ctttctggaa ttggattgga
     1501 agagggtctt caggtgcaac gatcatgtct cccgaaccag atacactgca aaaagtacaa
     1561 ccgccatggg caacagtccc atctcgattc ggacagtcaa atcctccatc gatcggcacc
     1621 ttgaaaattt tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg atatctttta
     1681 tttccttctg aataaggaaa agtcattaaa agtccttctt tctgtaagag ttatctattt
     1741 gttttctttt ttctctatac actggataag tcaggcacag ccaagaaaga ctgagtaagt
     1801 aaccgccgat gatatcagta ggaaaatgaa cgcccagata aatgcggctt atcccgacac
     1861 ttactataat aaacatcatc agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa
     1921 aagctggaag cagaaatagt aaagaaccgt aaagaatcat actgcctgct gcatgtccac
     1981 tagggaaact gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc tctcgcatga
     2041 aaaatagttt gatcaccggg ttgatgattc cagcaattac aataaatcca gaaacgaacc
     2101 aaaatgtttc tgcccttttt ttcttatacc acatccataa agccgcacaa gcaaataaaa
     2161 tgatgacggt aagaggatta ccgaatttag tcataaagag ttggtagctg ttcagatcag
     2221 gataagaagc tctgacaatg cctgtaaacc actgatcgaa accagaaagc cacgacagat
     2281 ggaactttac gatccagccc aaaatgaaaa acaggacaag aaagaagcta ccagccacct
     2341 gaaatgatgt tttatatttc atacaattcc tcctttgaag caatgactta ttatagcatt
     2401 tttggtccag cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc tctttgaata
     2461 accaatattc ttataaaaaa aataactgta atcggaaaga cattttttgg taatgcaaat
     2521 gaaatgttag ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg
     2581 taggaagaac ttgaaaatga acagaagttg ttcattacgg taaattacaa tatgattaca
     2641 aaaaacaaca aagctc
//
LOCUS       DAYYHS010000176         2629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000176 DAYYHS010000000
VERSION     DAYYHS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.183"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..622
                     /locus_tag="ACHA6A_002977"
     CDS             116..622
                     /locus_tag="ACHA6A_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8971086.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            639..1538
                     /locus_tag="ACHA6A_002978"
     CDS             639..1538
                     /locus_tag="ACHA6A_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8971087.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1625..2509
                     /locus_tag="ACHA6A_002979"
     CDS             1625..2509
                     /locus_tag="ACHA6A_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8971088.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT          958 a          489 c          515 g          667 t
ORIGIN      
        1 gtcacacctt cttttttgta tataaaaatc ccaagtaaat acataaaaaa taaaaaaatt
       61 gaaactttta gcgcaccttg tacgtcatat taagtgaggc agaaaggagt ggagaatgat
      121 ttttactgga tataagaaaa aacggcagat cgaaaaatac cgtcacctgc tgaatggact
      181 catcgaaact gattatcaaa aaatgttccg tatcacgatg aattacgtac atgataaaga
      241 agaggcctta gatgtgatgc aagatagttt tcataaagcc ctgaactatt ttgaaaaaga
      301 aaaagagatc gagcatttta gtgcatggtt ttatcggata ttgatccgca cggcattaga
      361 tagttggcgc agacaaaaaa ggacgcctgt ggacttattt gaaattcaag atatccccca
      421 acagttacct ttagaaactt ccgttgcaga gctccatatg attttagata aaatagacag
      481 tccggaaaaa gagattctga tcctctactt ttttgaagga tttcgtttga aagaaattgc
      541 gactatcttg gatatgaatg aaaacacagt taaaacgaaa atgtaccgct cattgaatgc
      601 tttacgtcaa atcctagaat gaaggagaga agagtgccat gtattcccaa aaagaaaatt
      661 taaaaaaact aaagaaaact tataaagaac aagcaattcc caaagggctt aaagaggaga
      721 tccgccagcg ttttattatc gaagagcaat catttataaa agcaaagcgg agaaagagac
      781 gtctccaagg gttcggagta tctttggcta tcgtaatgat tacaacaagt agccttcttt
      841 ttaatagtca agttcgaagc tttgcagaag atttacccat cttaggatca gtcattgaat
      901 tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat cgatattcag gtaccgaaga
      961 tcaatacaca aaatgagaaa gaaaataaaa caattcatgg attaaatcaa aaatactttc
     1021 gcgaaggaca agcagatttt gaaaaagctg aaaaagaata tggaaatttt gaaacggatc
     1081 attaccaagt tttgggagat tatcagaaaa tcgtagacga taaccggttt ctcgtgattg
     1141 aaaggcagat cacccaaaca gcagcagata gccatgttga aaaaagatac gacacaatcg
     1201 acaagaaaaa cagtgtccag ctctcacttc cacttttatt caaagatgac acctatctat
     1261 ctgttttgac aaaagaagtc aaacggcaga tggccgaaca agtcaaggaa gatccatcta
     1321 agtactattg gactgagcaa gatattcaag aagggacgat agaaaaacca acacttgtta
     1381 cgcctacacg aagtttttat ttgaacaaag agcatcaatt agttttgact ttttctcaat
     1441 acgaaattgc tcctggttat atgggtacac cagaatttgt cattcctaag tccgtgacaa
     1501 aaacgatttt ggcatcggaa gattatttgg atcattagac gagaagtagt tgcgactagc
     1561 agtttcaatt ttcaagtaag tacaagtaaa taataagaag agaaaaagag aaagaagaga
     1621 tatagtgaaa agaaattatt tagttatcag tattattgct ttatccgtgc ttttgttttt
     1681 cgaaattact tcggtggcct ccttgatcac cgaagtaaaa ggacaaacac actcttcgga
     1741 gcattcgtca caatctttca aacaatcaga acaatcaacc attaaaagcc aatcaactga
     1801 aacagctaac gtcaaagaag ccattcaaga atcagcggaa aaggtacaaa gtcagtcatc
     1861 acaatcaaca caagacagta caaaaacgag ccgagatccg gctgtcaaaa gcgtcgcaat
     1921 cagctttgat gatggaccag gagcgaccac aacaccgcag cttctgcgaa tcttaaaaga
     1981 aaaaaacgtg catgccactt ttttcgtctt aggagaaaat acggcgcagc atcctgaaat
     2041 cgtgaagcag acagctgaag cagggcacga gattggcaat cacacatacg atcatcagaa
     2101 tctggccact ctttctgccc agtcgatgac ggaggaagtg acgaaagcag acactgagat
     2161 caaaaaagtc actggcaaaa cccccacttt tgttcgtccg ccctatggtt ctgtcacgaa
     2221 tgtaggagca acgatcatcc agcgaccgat catcgaatgg tctgttgatt cagaagattg
     2281 gaagacaaga aatcctgatt tgattttgca gaagatccaa gcaacagtat acgatggggc
     2341 aattatttta ttccatgata tctatcctga aacgattcgt gccgttcctc aagtgatcga
     2401 ttatctgaaa gagcaagggt atcgcatcac gacagttggg gatttattag ggcatccaac
     2461 ggctgtcgag aactattatg gcagaaacga tcatcggcca gttcagtaag tgagacaagt
     2521 atctttaata aataacacat tttaataagt agttgaacac ataactacct tacttgcaaa
     2581 ggcttaacaa tatctataca ggtacaacaa aaaactgtcc accaatttt
//
LOCUS       DAYYHS010000177         2583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000177 DAYYHS010000000
VERSION     DAYYHS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.184"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2262
                     /locus_tag="ACHA6A_002980"
     CDS             <1..2262
                     /locus_tag="ACHA6A_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8971089.1"
                     /translation="LIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYF
                     NSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIV
                     EQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSV
                     LAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLE
                     TFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQ
                     SIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIE
                     LLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIF
                     EIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAE
                     HYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPEL
                     SVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDI
                     LIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRN
                     SANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPEN
                     GNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQAS
                     SYLFKFKQKRSDG"
     gene            2397..2525
                     /locus_tag="ACHA6A_002981"
     CDS             2397..2525
                     /locus_tag="ACHA6A_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8971090.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          987 a          339 c          495 g          762 t
ORIGIN      
        1 ttaatagaca aattagatac gaaagacatt gtcaattggc tcaatcaaga cgaagaattg
       61 acagatttat tatcttcggt aaaaaaagaa tcaagtgcaa gtcagcaatc gaactggagt
      121 ggattgtact ttaattcaaa tgagtatgtg aaatacattt tgaataaaac tgcatggatt
      181 aagattgatg gagaaaaata tagcccgaga caaattgtgc tatatgaaaa gctccaggca
      241 cacgttccgg gattgtatgg agtttctgaa caaagcttaa tggaactctt ggggcaacgc
      301 attgttgaac agttggattt cagaaaaaat atggcgatgt tttcggatga tgaaataaga
      361 gctatattag ttgagttacc aaaattcgat aaaggcgaaa taagtcgcag attatataat
      421 gaacttataa gaaataaaaa aggaatgaat cctacttatt ctaccgaagg attatcagtc
      481 ttagccaaag atggcttctt ttatccaaac aaagaattaa ggtatgctga taagcgactg
      541 cctaaatcaa tagaaaaaca aatgcgtttt atttacgtgc ctgaaaaagc tagtacaact
      601 acgatatatg aatggcttgg ggttgaacgc ttcaaaacaa atttaaaatt ggaaacattt
      661 gagttattgg aagattattc tgaggatttc aaaaaagaga ttgatgatat caaaactgcg
      721 gtattatcta cgatagatga caatagtaag aatgttagtt cattaaaacg tattcagatt
      781 attccatgca gcaaaatatc tgctaaagat gttgagcaag atggtcagtc aatcgttttg
      841 gaagattatt attttgtgga aagtaaagga aaattctact taaaattacc tgctaattct
      901 attgaaatta atcaattacg tttgattgat acgttttctt caacaatcgt agacatattc
      961 aagcaggtgt taaatctaag cttagatttg aatttgattg aattgttaat ttcccgagat
     1021 acggaacata aacaaagaaa aataactgaa gaattcggtg tagataagtg gaatgaatcg
     1081 tttgagctat tgtttagcga aaatgcgtta aataaaaaag ttaatgactt tttgttatca
     1141 aatggactaa atgaagagga atatcctacc atttttgaaa ttgactattc atatgcatta
     1201 aaagaagaag agtatcaatt gttggcgata gctctagaaa gcatttcaaa agatattgag
     1261 gatttgaatg gattgtcaga attgattcat ttggatattc gtgaattttg gaaactgaaa
     1321 ttaagatctt atctagataa gaaagcggaa cattaccgcc agattttata tagcaatgca
     1381 aataaaagcg gtgatgaaat actcaaagaa acattcctag aaaaaattga tagatacaaa
     1441 gattatcaat ttgagatttc tgaaattcca aattcagttc aagtcgacat tgagaaagaa
     1501 agtaagaaga gatttccaga gctaagtgtg tcaattactg atgaaattga cattgatggc
     1561 ctgtatgata ataatgtcga agagttagtc acggttgcag ctattacaat agatgatttt
     1621 gattattata ttagaggtca tcaaaaagtt tcttccttac tgtattttgg tattccgtcc
     1681 cgtttaagag aagatatact gatattcgtt actaaagaaa atgatgttga tttgaacgga
     1741 gaaaaatctg gtatctctat tgaaggaaca tcaactgttc aaaccattct tgaaccatcg
     1801 aatagtgtta gtaggaaagg taagaacaca cgaaatggag aaagaagtaa acgagattat
     1861 gataatagaa actccgcaaa tgatgatgct ggtgaaacag cagaacaaat tgcttacgct
     1921 gaattgactc agaatcaaaa acttaatgta atttggaata cgaaatattc tagtaaatct
     1981 tcagatcgta ataagcagcc accaaacgga attgtttgtg atatgtgggt tcatgaccca
     2041 gagaatggga atatgtactt tgaggtaaaa tcatcaatta cagaatttga aatgtcgatt
     2101 aatgaatata attctatgtt agataaccct gactcttacg aggtcgtttt agtcaatcgt
     2161 gataccaaag agataagtcg tcataaattt gatgaacttg atggattgaa acaggcaagt
     2221 agctatcttt tcaaatttaa gcaaaagaga agtgacggtt gagtgatttg tttccacaaa
     2281 cctattcaat ctatcaactc aaaaatcata aacaaccatt attatagtag gaagatacag
     2341 agtgtactct caacgcatat cgaattagtc gcttcattcg ttaaaggaga ataaaaatgg
     2401 accaaaaaga aaaaatgaaa gaattgatca aaaagaaaca aggtggaggc cgatccaaac
     2461 aaatggaaac acctaagaat gatcgtaaaa acatgagaaa aggccctaaa atctttaata
     2521 aatagacgtg ctaaagaaag actaagaagt gttaactttt gttattgttt cttagtcttt
     2581 tta
//
LOCUS       DAYYHS010000178         2565 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000178 DAYYHS010000000
VERSION     DAYYHS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.185"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..400
                     /locus_tag="ACHA6A_002982"
     CDS             188..400
                     /locus_tag="ACHA6A_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971091.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(678..2477)
                     /locus_tag="ACHA6A_002983"
     CDS             complement(678..2477)
                     /locus_tag="ACHA6A_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8971092.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          927 a          455 c          468 g          715 t
ORIGIN      
        1 ataggaatat aaacaaacgt ttttgtcaca acgtctaaga tcgtgttaaa aaatagttat
       61 ctaaaaagca gaaagatact caaaataaaa aataaaaaaa tgaataaaaa cgttttcaaa
      121 tacaaggtaa tttgttatta taaaataaag aaaaaacaaa ataaaaataa agaaaagagg
      181 gaatgaaatg gtaaaagcag aagtaggaag atcttatata tgcaaaccga tcggagtatc
      241 tcatgaacta atcggctgta ttgaacgtac atatatcaac acggctgtcg tgtatgttga
      301 aagttatgac caacgagact atcaacttat caatgaaagc aagtatcgca tgctcgtaaa
      361 attctgtgat attagcgcac ctactgaatt ggtttcttaa acagtatttt tgagaattcg
      421 caagaactcc cttataatat aaataaagac agaagggagc gagaagatat gccaaaaaga
      481 aaaagttcag ggaaaatggg aattattctt gcagtgatct ctgcaattcc tgctatttta
      541 gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag cacacattat gagcgtcaaa
      601 caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt ttgttgtata aagacttttt
      661 tcattaacga tttttgatta gccgactccc aaaacaaaat ttagcagttt atctgaataa
      721 gtgatttctt catctgtttg tttcacactt tcatttagta gtcccatctc atcggttatg
      781 ataccatcca caccataaaa catcatgcga ctcatggcat ctgaatcgtt gattgtccat
      841 acatatactt cttttccgtc ttggtgggca gaattaatga agttacggtt gatcgtacta
      901 tattccattg tcagaaaatc agcaggcgtc acagggggcc caacaatatt gaaaggtaag
      961 atgtagccca cataaaagtt tggttctgct tcttttaaat ctgaaacggt ttggtacgtc
     1021 aaactttgca aaatatgatg gttctctaag ataactttgc ggtatttatg gacaaatcgt
     1081 tcgacaaggt catccgaatc ttttttagag gtcttgatct caataagtaa tttttggttt
     1141 agcttatgtg ctttttccaa ataagaatca aaagaacaaa caggggcttg cactccgttt
     1201 tcagtaactg taagatttgt caattctttc aatgtaagat cctgtggtgt tccatttacc
     1261 cctgttaagt gacgaagatt tgcatcatgc atcactacga aatgaccatc ttttgtttcc
     1321 tgaacatcca tctctacata atcaggatga tagtctctgc ttgttttttc aagtgcttct
     1381 aaagtatttt gcgccccatt ttgggtactc acacctctgt gagagacagt cactggtttt
     1441 gtctgaacag ctgaagtcag ataatccata ttgtaaagac agacgccaat accgaaaaaa
     1501 actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga aacgcaagtt gggggcttgt
     1561 gggataaacc acttagggat ctcaggcaag aagccttcgt catccatgaa atcaacaatg
     1621 atataaaaaa tccctacggt agacatcacg atattgaata ataaaatccc ttgtaaaagc
     1681 gtcatagcaa acacagcgct gatcaatgca tagtctggga aaaattgttc aaccatactt
     1741 tgtgccaaaa taacaacgat atatcctaaa gaagaaagga gtaaaatcgt accgccaatc
     1801 acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg tcaatgacca gctttcccga
     1861 atggctgctc gaaaagaacg atctcgtaga atcatttccg gtaaagcaaa aatcagtcga
     1921 atcccaatat agccaaggaa aatataaacg agtaaaaagg aactgacgat gatccaacga
     1981 ttagcaaaaa taaaatccat aatgaaagcg gggattttta tttttgacag tagatcagaa
     2041 ttaaaactta atccgctgat cggtagaatc aataaaaaat aagcaaggaa aaacaaaaaa
     2101 gtaatgggtc tcactttctt cagctgaagt atcgtcaagt gtaagagttg cttcaaagag
     2161 attggttctt tctttttaat aaaaaaaaca ctcatcaaaa gaaaagtaaa ttcgaaataa
     2221 actaacagta aaatcgtcat caaaactacg attaacgaga gtagaacccc aggatgttga
     2281 gaaaaaattg aaggaatatt gtcataagac aaatagtcaa ttgcccctcg gtgtaaaata
     2341 aaacgggtcg tactgctcaa taagggtaaa caaataaata aaataaagcc atgcatcagc
     2401 aagacatcac ggaaataagc ttgtgttcct tttaaaaagt cccaagcatt acctaagcta
     2461 cgtttcaaat aattcatcct atcacctgct cttcacattg tagcgcaaat ctaatgaaaa
     2521 aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat ttaac
//
LOCUS       DAYYHS010000179         2507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000179 DAYYHS010000000
VERSION     DAYYHS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.186"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2360
                     /locus_tag="ACHA6A_002984"
     CDS             207..2360
                     /locus_tag="ACHA6A_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8971093.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          865 a          430 c          548 g          664 t
ORIGIN      
        1 taagaaaaca taattttcaa aaataagttt attttttaat gaaaaaaata gttggatatg
       61 taactttaag tgattgctgt gaaacaaaaa gttgatttta tcttgaaatt aggtaaagaa
      121 tttcttgctc cgcccatttt ttctttttta ttaagaagtt tttgggtaca atgaagcatg
      181 aatgtaaata ggcggaagga taggaaatga tgaaagattt tatgaaaaaa atattaaata
      241 cttcttggtt agaaaaaata agaaaaggtg cacaagaacc taagaacaaa acgtttcgcc
      301 ggtcccatgt tccttttcgc ttaaattttc tttttttcat tatatttacg ttatttgttg
      361 cgttgattgc tagattgggt tatttgcaga tcgtgaacgg cgaagagatg gaggcgcgag
      421 tgaagtctac ttctacgatt acggtccaag aaagtactcc tcgcggaatg atctacgact
      481 ccacaggaaa agctttagtt acgaataaag cgaatcaggc catcacatat acgagaggaa
      541 cacaagttac ggctaaagat ttgcttgaaa tcgcaacgaa gctgaactcg ctgatcgatg
      601 ttccggtaga tgaaaatctg acagatagag ataaaaaaga tttttggctg gctgatccca
      661 aacatttgaa agaagccaca aaaagacttt cggcaaaaga aaagcaatta gggaacagcg
      721 aacaatatag tgcgatggtc aataaagtca cagctgatga gattaattta aacgaggagc
      781 aattaaaaat tgctaccatc tttaaacgga tgaatagtgc atatgaatta aacactgtct
      841 atatcaaaaa ctctggtgtt actgatcaag agttagctgt tgtggcagaa cgcgcttctg
      901 aacttccagg agtttcgact ggcacagact ggacacgaga atataatgca gcaagttcac
      961 tgaaaagtat tcttggcagt gtgacgactg aaaagcaagg acttccagca gatgaagcag
     1021 agaaatacct agcaaaaggt tattctcgga atgaccgtgt aggacaaagt tatttggaaa
     1081 aacaatatga agatgtcctg caaggaacca aaacccaata tgaagtttcc cttgataatg
     1141 aaggaaatgt ttcgaatcaa aaagaaatct tctctgggga aaaaggttct aatttaatgt
     1201 tatctatgaa tgcagaattc caatcaaaag tagaagaaat tttgaaaagg aattatcaaa
     1261 cattgatcaa caacggcaaa gcacagtatt cgcctggtgc ttatgcagtg gctatgaatc
     1321 ctcagacagg agaagtgcta gcaatgactg gtttcagtca tgaacaaggt tccaaagaaa
     1381 tcaccgagaa tgctttaggc acgattacta gcgcctttga tccagggtct gtagtcaagg
     1441 ctggtacttt gacagcaggc tgggcaagta acgcgatcag cggaaatcaa gttttgattg
     1501 atgagccgat tcgtctgcaa ggtgcttcag aaaaaagctc tgtctttaat cgaagcggac
     1561 aagttgctct tgatgcagtc aaagcatcgg aattgtcttc taatacgtat atgatcaaag
     1621 tggcgttaaa gatgttagga ctagactata cacctggaat gggactacct agtttagatg
     1681 aagaagctaa agcttatcag caattgagag actctttcaa agagtttggt cttggtacaa
     1741 cgactgggat cgatttgccg aatgaatctc ctgggatctc acgttctgtt gattatatga
     1801 agaagttcaa tgcggataat ggcaaagaat ggtatgctcc aggtaacttt accgatttgg
     1861 catttggaca gtttgacaca tatacaccaa tccaattagc tcaatatgct tcaacagtcg
     1921 ctaatggcgg gaaacgagta cagccgcacc tagtcaaagc gatctatggc aatgacgaaa
     1981 atggaaactt gggtgaagtg aagaaagaaa ttggcacaac agtagaaaat acggtcaata
     2041 tttctgcaga aaatatggcg atcctccgtg aaggattcca ccaagttgtc catggtaccg
     2101 atccttacac aacagccaaa ccattagcca gtgcgaaaat ggacctttcc gcaaaaacag
     2161 ggactgcaga aacggttgcg gaaggtcatc cagatattgc gacagtcaat agtaatatcg
     2221 tggcatacgg accaacagac aatccggaaa ttgccatcag tgttgtcttg ccgaacttat
     2281 tagacgaaca agaccacata aacttgacga ttgctaaaga aataatggac gcgtattatg
     2341 atatgttcat ggcaaaataa aaaagaggtc gtgaaaaagc cgcttagttt cgagtatgaa
     2401 gaaaaatttt gtgaaaatag cttctcacat ttttgcttaa atttgacttc ataccgagaa
     2461 actggctttt gaacaccgtt tactcggcaa aaaaggttgt gacaaaa
//
LOCUS       DAYYHS010000180         2389 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000180 DAYYHS010000000
VERSION     DAYYHS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.187"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACHA6A_002985"
     CDS             197..2059
                     /locus_tag="ACHA6A_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8971094.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2389
                     /locus_tag="ACHA6A_002986"
     CDS             2059..>2389
                     /locus_tag="ACHA6A_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8971095.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          352 c          464 g          713 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggattt aaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DAYYHS010000181         2125 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000181 DAYYHS010000000
VERSION     DAYYHS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.188"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHA6A_002987"
     CDS             <1..963
                     /locus_tag="ACHA6A_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8971096.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ACHA6A_002988"
     tRNA            1152..1225
                     /locus_tag="ACHA6A_002988"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
     gene            complement(1740..2039)
                     /locus_tag="ACHA6A_002989"
     CDS             complement(1740..2039)
                     /locus_tag="ACHA6A_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8971097.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          708 a          375 c          379 g          663 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1441 taataaagaa gagtttaaag cgttccccaa aaaccactgc cccataagtg tgttacgctt
     1501 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     1561 aaaaaggctt ataattggag tggtagttaa ttagtgactt atttttgatt ttatagcact
     1621 gatactataa aatatagata tcatcatatt acacaatctt aatactaact taaaaaatat
     1681 ctcctttcat aagtatggtg ataaaatccg ttccgggcta cctttttagg tagcctactt
     1741 taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga
     1801 tcgcttcgag cgtctagcac atcagtcacg atatcaaaca aaaaatatac gtctttcttc
     1861 attctagttt tcgcagcagg gattttaaag tagttcttat tagaatagta gagattaatt
     1921 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
     1981 ttgacaaatc tattcgaagg atcaaattta aaccataatt gcgtctcatt aaaaagcata
     2041 accattactc cgatctattt ttaaacttag tctctacctc taatatatat cgagttttta
     2101 ttttgccttc agagaatact gtttc
//
LOCUS       DAYYHS010000182         2084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000182 DAYYHS010000000
VERSION     DAYYHS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.189"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..1031
                     /gene="traE"
                     /locus_tag="ACHA6A_002990"
     CDS             699..1031
                     /gene="traE"
                     /locus_tag="ACHA6A_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8971098.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1650..1991
                     /locus_tag="ACHA6A_002991"
     CDS             1650..1991
                     /locus_tag="ACHA6A_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8971099.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          730 a          278 c          435 g          641 t
ORIGIN      
        1 tattatgata aaaatcacaa acgtagcctt ttacgtcata agtgttaaaa gaaataatta
       61 atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga gttataataa
      121 tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata ttacttcttt
      181 tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg tctgctatgc
      241 ttgtgatgaa ttgattttcg ttttgcactt ttggatgata aatttggaca attgaaaata
      301 caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag cgttcataca
      361 cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat tacccgcaat
      421 ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg ttaaggttaa
      481 aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa tgtccgtgtg
      541 gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga tgcgttccct
      601 cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac
      661 ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca ggaattttga
      721 gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga
      781 aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag
      841 acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata attttaaaag
      901 atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg
      961 tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt
     1021 tgatcggctg aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa
     1081 atattgtcga gacttctaaa ggaacttaga gtcactagaa aaataaggag tagacttgat
     1141 ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt aacgtaaaaa
     1201 attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa
     1261 taagcgcaga gtaaacaaga gggaatttag aagttagaca agttagaata gcgtctagat
     1321 taatcatgag cagctactaa gggatctaga ctttgacgca aactcatgga ataaggagta
     1381 ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag
     1441 atacttgcga ggacgttgat tattggatca atcgagcatt taccgctgtc tactagggtt
     1501 tctcgtttcg gacgccataa acgagtcgaa attttttatg tatttttaag taaacgaaaa
     1561 acgataaaat atgaacaagt cctgaataaa gtgaaaaaat tgatataaaa catagagaaa
     1621 atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat atacgattgg
     1681 gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt gtgacgttta
     1741 gaaggggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat gatttatacg
     1801 atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat gatgaaggta
     1861 atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct gttgaagata
     1921 cttggtcata tttgagtgat tatattgtca gctttgaaat cattgaattt gtagcggata
     1981 aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag gagtcacgac
     2041 gattaataaa aaggtagtat taccttaggc attgagtaag gaat
//
LOCUS       DAYYHS010000183         1979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000183 DAYYHS010000000
VERSION     DAYYHS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.190"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..429)
                     /locus_tag="ACHA6A_002992"
     CDS             complement(<1..429)
                     /locus_tag="ACHA6A_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8971100.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKF"
     gene            complement(426..1079)
                     /locus_tag="ACHA6A_002993"
     CDS             complement(426..1079)
                     /locus_tag="ACHA6A_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8971101.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1367..>1979
                     /locus_tag="ACHA6A_002994"
     CDS             1367..>1979
                     /locus_tag="ACHA6A_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8971102.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          624 a          351 c          253 g          751 t
ORIGIN      
        1 aaatttaagg cttaaatctt tcgcatcttt ttgcatatca gctgtagaaa caaattgtag
       61 atttgttttt ttaccatcat taagctttat ttctctttct tcaattatca aagaattact
      121 atcactttct aaattaacat ttatattatc tttgatttgt atttcattct tattattttt
      181 ttcttttatg aatactttta tttcactact cttggaataa aactccaaag tttgttctat
      241 atattttatt tcttttccat tcatattact agaaatttca tttgccatat tatgaatctt
      301 ttcttttctc gtctccaaat atgttttagg aaagataaaa aaaattaatg aatgaactaa
      361 tataattatt attccaagaa cagaaaatat ttgtatgaac atttttggaa atatctttaa
      421 ttttttcatt ttctctccaa tttatatcct acatttctta tagtagttat acagtctaat
      481 tgtaattttt ttctaagttc ttttatatat acatctatta ctcgatcata aggagtttct
      541 tcgctatctt tccatacata atcaataatt tgcattcttg ttaacacttg tccattatta
      601 tccaataaac attttaatac ttctagttct tttgcattta catctatttc ttcaccattt
      661 attttagctg tatagctatt aaaattaacc gatagatcct tatattcaaa cttctctaaa
      721 tgtccataat tcttcttaat taaagaatct attctagctt ttaagaccgg caatgaaaat
      781 ggcttttcta catatccgtc tactaaatta gtaaatgcat caattttata ttcttcatca
      841 ctaaatgcag tcaagattag aattggtaaa ttgcttttct ttctaatttc tttcaacact
      901 tctaaaccat ttataaaagg tatctgaatg tctaagataa ccaaatttat atcgctatta
      961 aattttgaca aggcttctct tccatcttta gcttgaataa cagtatatcc aaattctgaa
     1021 agatattcac ttattccctc tcttatcata ttatcatctt caacagttaa tattttcata
     1081 tatacctcca cttaagccta tacactagat attatacagc atattatggc tctataatat
     1141 ctagtgttgg tgacaccttc tgttgccaat actttttctt ttttctaata aatgcaaaca
     1201 gacaccttga aatcctttag aataaagggt tctataatat tttgtgttgt tggaaaatcg
     1261 aaggcgattt tctactaaca ctttttcttc gtttcaactc aaaaagagac tcacttttgg
     1321 taaaatttag tcgaccaaaa catcacccta ccaaaggagt cgctctatgg ataatcatac
     1381 tagaaaatta ctcgtattaa cagataaaca tttatttttt gatgaagaat ggctgattga
     1441 aaaagaatat aaaggcgttc aagcgcagtt tattaaaggg agattggatg actcaccttc
     1501 ccattgtgaa cactgtggat cgattcgtat tatccgcaac ggttcctata cgacacgtac
     1561 tcagatattg aaagtcaaag aaaagttgac tattttagaa ttaaaacgta cccgttttct
     1621 atgctatgat tgtgggcaga ccttttctgc gaaaacggac ttagttgatg aacatcatca
     1681 actaacaaag gaactgaagc aggcgattct catagaactg tacgaaaatc aatcacgtaa
     1741 actaatcgct aagaagtact tcgtctcgga tgggacggtg acacggatct tgcgtgaagc
     1801 aacgaagcat taccagccac gtatgaactt ccttccgacg gtgttgtgta tggatgagtt
     1861 caaatcaatg aagtctgttt ctggttccat gagttttatt tgtgttgatg ggacaaccca
     1921 tcagctattt acgatccttg aagatcgcag actttacaaa ctaactcaat acttcctgc
//
LOCUS       DAYYHS010000184         1941 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000184 DAYYHS010000000
VERSION     DAYYHS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.191"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACHA6A_002995"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACHA6A_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8971103.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACHA6A_002996"
     CDS             852..1040
                     /locus_tag="ACHA6A_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8971104.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACHA6A_002997"
     CDS             1053..1610
                     /locus_tag="ACHA6A_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8971105.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACHA6A_002998"
     CDS             1642..1884
                     /locus_tag="ACHA6A_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8971106.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          714 a          300 c          400 g          527 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga c
//
LOCUS       DAYYHS010000185         1911 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000185 DAYYHS010000000
VERSION     DAYYHS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.192"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHA6A_002999"
     CDS             <1..969
                     /locus_tag="ACHA6A_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8971107.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACHA6A_003000"
     tRNA            1159..1232
                     /locus_tag="ACHA6A_003000"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          639 a          327 c          362 g          583 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta gactagacag
     1861 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat g
//
LOCUS       DAYYHS010000186         1906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000186 DAYYHS010000000
VERSION     DAYYHS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.193"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHA6A_003001"
     CDS             complement(<1..426)
                     /locus_tag="ACHA6A_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8971108.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHA6A_003002"
     CDS             complement(535..1566)
                     /locus_tag="ACHA6A_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8971109.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          586 a          320 c          328 g          672 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaa
//
LOCUS       DAYYHS010000187         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000187 DAYYHS010000000
VERSION     DAYYHS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.194"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHA6A_003003"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHA6A_003003"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8971110.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYHS010000188         1827 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000188 DAYYHS010000000
VERSION     DAYYHS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.195"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..459)
                     /locus_tag="ACHA6A_003004"
     CDS             complement(145..459)
                     /locus_tag="ACHA6A_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971111.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            745..984
                     /locus_tag="ACHA6A_003005"
     CDS             745..984
                     /locus_tag="ACHA6A_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971112.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1346..1606)
                     /locus_tag="ACHA6A_003006"
     CDS             complement(1346..1606)
                     /locus_tag="ACHA6A_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971113.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          640 a          310 c          238 g          639 t
ORIGIN      
        1 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
       61 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      121 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      181 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
      241 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      301 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      361 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
      421 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
      481 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
      541 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
      601 tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat
      661 aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac
      721 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
      781 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      841 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      901 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      961 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
     1021 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
     1081 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
     1141 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
     1201 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
     1261 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
     1321 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
     1381 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
     1441 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
     1501 caacacaatt ttctaattta ctgattactt ctccaatcac tttttgtttc aagccaatag
     1561 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
     1621 taatgttttt tcattattta aaatgataat aacattaaaa tatgattatg aaaatcaata
     1681 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctttc
     1741 cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta
     1801 ataaatgaaa aagcatttat attaaaa
//
LOCUS       DAYYHS010000189         1812 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000189 DAYYHS010000000
VERSION     DAYYHS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.196"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..667
                     /locus_tag="ACHA6A_003007"
     CDS             <1..667
                     /locus_tag="ACHA6A_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971114.1"
                     /translation="SKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVKNIKKIPK
                     IDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKE
                     TLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKT
                     INSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEY
                     GII"
     gene            654..>1812
                     /locus_tag="ACHA6A_003008"
     CDS             654..>1812
                     /locus_tag="ACHA6A_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8971115.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGII"
BASE COUNT          613 a          228 c          286 g          685 t
ORIGIN      
        1 aagtaaaaac gttcaaagag tttttaaaat attaaataaa aatagcatct tcaaaaaaaa
       61 aattgacgat tattttgtct attgtccgga attgttactt tatgatgtaa aaaatataaa
      121 aaaaattccc aaaatagatg aaaacagtaa attgatgaaa aacatacaat caatctttgg
      181 gatagaagaa gtttttgtcg gagataaatg tatctttttt gatcaacctt tattatctga
      241 tggttatggc atagatgaaa cagatatttt cgctaaagta aatagtatat tttctggaaa
      301 aaaagaaact ttaattaagc ttcatcctag agcaaggaca aatcgttatg gtgaaaaaaa
      361 catattgaat tccagtttac cgtttgaagt cttgctactt gaaaatgact tctcttcaac
      421 acttttgatt agtcctatat ctacaatttc tttttcacca tctatgatgt ttgaaaagac
      481 gatcaactct attatgttgg ctaaattaat tttaaatgaa tatacagcat tatctgaaga
      541 taaaaaagaa acattgacaa atattgttct tttctgtgaa cattataacc gtctatcaga
      601 agaaaaaatt ttactcccag aaaactgggc agaattaaga aagttggtag agtatgggat
      661 tatttaacac actttccaag tcaaaattag ttaagtccag tttctggtat actataggga
      721 atttttttat taagggaatt tcattcataa ctattcctat ttttgtaaat attactactc
      781 aagcagaata tggtttgatt aataatttca cttcatttgt atcaattttt tcgattcttg
      841 ttgggctaag cctaaattcg gcaattaata atgcaagttt tgactataaa gatgacataa
      901 atgaatttat gtcatcggta ctttctctag ctacactttc gttaattgtg ttcttattaa
      961 tagggaacat atattttttg ttttcaaatg ctttttttga tatgaaccaa cttgtattta
     1021 atttgatgtt attacaaagt tttgctagtt ttgtaattag ttatatccag tcttatttta
     1081 cgattaactt taaccatttt aaatatttga ttttatcttt tatatctact tttttgaata
     1141 ttgcaatctc tgtagctttg atgcagacat tatattttga tgataaatat gttggcagag
     1201 tagtcggcgg aacactgtca tttgtgttga taggtttagt aatttattta agaataatga
     1261 taaagggtgc tcattttttt gataaaaaac agtggagata cgctttaaaa atctctttgc
     1321 cgctaatccc acattcctta gcaaatatag ttttagcaca attcgataga attatgatta
     1381 attcttattt aggtcctgct gcggggggaa tatattccta cattagtaac ataggtatta
     1441 ttctaagcgt aatatggatg tctactaata acgcctgggt tccatggttc tatggtgaga
     1501 tgagtaaaaa taatgaaaaa aaagtgaaga aagttagtaa ctattattta gtagtcttta
     1561 ctttgattac gatgattgta atggtagttt cgattgattt agcaaaattt atggctccga
     1621 aagactatta ttctgggtta tctttagtgg ttccgataac cttaggctat ttctttcagt
     1681 ttttatatag tttaccagtt aatgctgaat tttatgagaa aaaaacatcg tttattgcga
     1741 ttggtacagt agcaagtgct atagttaata tactattaaa catgattttc atacctaggt
     1801 taggaataat tg
//
LOCUS       DAYYHS010000190         1777 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000190 DAYYHS010000000
VERSION     DAYYHS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.197"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACHA6A_003009"
     CDS             complement(37..993)
                     /locus_tag="ACHA6A_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971116.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1118..>1777)
                     /locus_tag="ACHA6A_003010"
     CDS             complement(1118..>1777)
                     /locus_tag="ACHA6A_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8971117.1"
                     /translation="VSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSR
                     GVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKA
                     VYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLI
                     ESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFT
                     E"
BASE COUNT          547 a          298 c          291 g          641 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aacttgggag cgtcaataat
     1081 tttgtgtaaa taaattgtcc tcctgcaaaa taattagtta ctcagtaaac attgaaacta
     1141 atgtatcggt tacctgttga aaacctttat ggcttctgtt tagaaatttt tgattgtatg
     1201 tatcaaaaat gctgactaga aagcgttcta gtgattcttc attttgaaac tgctcttttc
     1261 tacggctgta tcttttaatt tgcttattga aagactcgat tagattggtt gagtaaatgg
     1321 ttctacgaat gctaggtgga aaatcataaa aagttaataa gtcttggttt tctatgagtg
     1381 actgcgtcac tttaggatag tttttcttcc atttctcaat catgccggat aagaaggtat
     1441 tcgcttcttc ttttgagtta gcttgataaa cagccttaaa gtcatcacag atttcttttc
     1501 ggtctttgac acgtacttta tgagcgatgt tacgagatac atggatacaa caatgctgat
     1561 attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag ccgtccgtaa
     1621 taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc tcattccaaa
     1681 cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg ccgtcttctc
     1741 gtataccaat ggcaatataa atcgcttctt tggatac
//
LOCUS       DAYYHS010000191         1756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000191 DAYYHS010000000
VERSION     DAYYHS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.198"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1557
                     /gene="guaA"
                     /locus_tag="ACHA6A_003011"
     CDS             28..1557
                     /gene="guaA"
                     /locus_tag="ACHA6A_003011"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8971118.1"
                     /translation="MIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPKG
                     IILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKA
                     ELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMY
                     GIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGL
                     SGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKE
                     RFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTE
                     TAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGL
                     GIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTY
                     DYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEW
                     E"
BASE COUNT          537 a          364 c          373 g          482 t
ORIGIN      
        1 ccaacacgat ttgacgaaga gccaaaaatt atcgtacttg attacggtag tcagtataac
       61 caactgatca ctcgccgtat tcgtgagttc ggtgttttct cagagttgat gagccaccgc
      121 attaccgcag aagaagtaaa ggcaatgaac cctaaaggaa tcatcctttc tggcggacca
      181 aacagtgttt atgatgagaa ggcctttggt attgatgaaa agatttttga tttggggatc
      241 ccagttttag gaatctgtta cggtatgcag ttgattactt ataaattagg cggaaaagtt
      301 aaaccagcca aaaaccgtga atacggaaaa gcagaattgg aaatcacttc tgcagaagcg
      361 gcccttttcc aaaacactcc tgaaaaacaa accgtttgga tgagtcatgg tgacctagtt
      421 acccaagcac cgactgattt cgatgtcgtt gctaccagca aagattgccc catcgcatcg
      481 atccaaaatg cagaacgcaa aatgtacgga atccaattcc atgcagaagt tcgccattcc
      541 gaatatggaa atgacttgtt gcgccatttt acactagatg tctgccaatg tactggtgac
      601 tggacaatgg ataacttcat cgacatggag atcgcaaaaa ttcgtgaaca agtcggtgac
      661 aaaaaagttt tattaggcct ttcaggcgga gtagattcct ctgttgttgg tgttctttta
      721 caaaaagcga tcggcgatca attaacatgt atcttcgttg atcacgtatt gttgcgtaaa
      781 ggtgaagcag aacaagttat ggaaagtctt ggcggaaaat ttggcttgaa cattataaaa
      841 gtcgatgcca aagaacgttt cttatcaaaa cttgctggcg tgtctgatcc agaagaaaaa
      901 cgtaaaatca ttggaaatga atttgtatat gtcttcgatg atgaagcaac caaacttgcc
      961 ggtgaagaag gcgtttcttt ccttgcacaa ggaacccttt ataccgatgt gatcgaatca
     1021 ggaactgaaa ctgcccaaac catcaaatca catcacaatg taggtggatt acctgaagac
     1081 atgcaattca agctgatcga gccattgaac acattgttca aagacgaagt acgtgaatta
     1141 ggtacccaat taggcatgcc tgacagcatc gtttggcgcc aaccattccc tggaccaggc
     1201 ttaggtattc gtgtccttgg tgaattgaca gaagaaaaat tagaaatcgt ccgtgaatca
     1261 gacgctatct tgcgcgaaga aatcgccaat gccggtttag atcgcgacat ctggcaatac
     1321 ttcaccgtcc taccaggcat tcgctctgtt ggggttatgg gagatggccg tacgtatgac
     1381 tacactgtag gtatccgtgc tgtcacttct attgacggca tgaccgctga ctttgcccgt
     1441 atcccttggg aagtcttgca aaaaatcagt gtccgtatcg taaatgaagt cgcacacgtt
     1501 aaccgtatcg tgtatgatat taccagcaaa ccacctgcaa cagtggagtg ggaataaaga
     1561 gccaacataa tatggatgag gaatacctat aaatcaacat tattcaatta ctaaagttcc
     1621 tgattttcgt aaaaaataag atggtttcct accattttcc ctaccaaagc ccttgttaga
     1681 ttaactacta acaagggctt tgcttttatt cactaccttt attttcctat tgaattagct
     1741 ttttatctaa tcagat
//
LOCUS       DAYYHS010000192         1748 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000192 DAYYHS010000000
VERSION     DAYYHS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.199"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..481
                     /locus_tag="ACHA6A_003012"
     CDS             182..481
                     /locus_tag="ACHA6A_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8971119.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(517..888)
                     /locus_tag="ACHA6A_003013"
     CDS             complement(517..888)
                     /locus_tag="ACHA6A_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971120.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(890..1291)
                     /locus_tag="ACHA6A_003014"
     CDS             complement(890..1291)
                     /locus_tag="ACHA6A_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971121.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(1305..1712)
                     /locus_tag="ACHA6A_003015"
     CDS             complement(1305..1712)
                     /locus_tag="ACHA6A_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971122.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          583 a          293 c          237 g          635 t
ORIGIN      
        1 atcaaagcag taaaaaagat aaattccaga tggatgtttc aatcttagct atattagctt
       61 tttccgaaaa taaacaacct gtaactgcaa aaaaagaaac agtattctct gaaggcaaaa
      121 taaaaactcg atatatatta gaggtagaaa ctaattttaa aaacagatcg gagtaatggt
      181 tatgcttttt aatgagacac aattatggtt taaatttgac ccttcgaata gatttatcaa
      241 agatttttat aaagtatgga attcagaagt tttcttttta gcaatcgaag atagcttatt
      301 aatcaatctc tactattcta ataagaacta tttcaaaatt cctgctgcga aaactagaat
      361 gaagaagggt gtatactttt tgtttgatat cgtgactgac gtgccggacg cacgtagcga
      421 tcatcggcgt tatgactata taaagtatac tttcgttgat ccagaaagat acaaagatta
      481 aataaaaaaa taacaaacaa actcgcttga aattcattag gtgagtttgt ttattatttt
      541 ttctgcttct cgattcatca gcgccatatc tgttttcgta atggtttcat taggaatata
      601 gtcagcacat tttcttttct ctaacattct atacccacta ttatgtattc ctaaggtatt
      661 caattcctta actgttgaaa acaaattcca aacggtttgg tggcttcctt ttccgctact
      721 tccattataa ataatatcat aattttcttt caaatactca tcagattttt taaaagcact
      781 gtagtatgct cttgaaacag cagttcttct ataagattct ttagaattat atttatcatc
      841 tgattcaatt tgtttggaaa tagataaata ttcattaaag tcaaacattt tattccccca
      901 aagttgctac tacagatttc cccatatatt tatagtcaga taatacatta tcgagagtat
      961 ccaaaacatc gtaatctgag gaaaacaatt cagatgttgt tttgataaca aagaaataca
     1021 agtttaaatc ttcatcatat tttatattga tttcagcttt tctttttaaa accgtattta
     1081 tcacatcaaa aattttgcca tcattacttg aaattgtgtt aatctcattt tttgtagtaa
     1141 ttacaagttg attatcattt ttagttttcg gagtagaact tgcatccaca acaactggag
     1201 atgctagaat taccgatgcg caagcaaaaa tcgcagcaaa atttattaag ctaccactcc
     1261 gatgtgtatt tgatggtact atatcaagca tccgtatcgt ctccttaatt tttttccact
     1321 ggtttgttga ccttgatatc aaagatttgc cttccaatct cagttccatt aaatttaaca
     1381 acagcataat gctctcctgc ttcttcaacc aagacatttc tggcgtttat attaaagttt
     1441 atatcgttaa cttgaggagg caagcttacc gatacttcac ccgtagaaaa aatttgtttt
     1501 tcattcttgt ttaaaacttc aatttctact atttttatct tttctgtatc ctctatctca
     1561 gacaccacag ctgttacaga aaatgataaa gcagtaggaa ttaaaggcgc attaatagaa
     1621 agagctggat taattacttt attaataaca ttagtgccaa attgctgact ttctacatct
     1681 tctgaaatta ttaattgtac tctataagac attgatttct cctaattttt acatttattt
     1741 aataaatt
//
LOCUS       DAYYHS010000193         1694 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000193 DAYYHS010000000
VERSION     DAYYHS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.200"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1420
                     /locus_tag="ACHA6A_003016"
     CDS             <1..1420
                     /locus_tag="ACHA6A_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8971123.1"
                     /translation="KYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTI
                     DTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLT
                     DNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNR
                     SNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIV
                     VTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGT
                     TYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQI
                     VTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEP
                     TNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTIS
                     GGLILLGTLGLLGYKNRKKENE"
     gene            1554..>1694
                     /locus_tag="ACHA6A_003017"
     CDS             1554..>1694
                     /locus_tag="ACHA6A_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8971124.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSL"
BASE COUNT          669 a          254 c          364 g          407 t
ORIGIN      
        1 taaatatgaa ccaacagtag aaggcgaaaa agtagaagtt gggggcacag tagatctaac
       61 agataatgtt actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc
      121 cggcggtaca attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta
      181 tccagacggt acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga
      241 cgccgataaa tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga
      301 tttaacagat aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt
      361 tactcccggc ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt
      421 aacgtatcca gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg
      481 ttctaatgca gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga
      541 gccagatgca tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc
      601 ttggaaagag aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac
      661 atatccagat ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc
      721 cgatgcagat aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc
      781 agatgcatct gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg
      841 gaaagaaaaa gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata
      901 tccagatgga tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc
      961 gaataaacct caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga
     1021 accaaatgca gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga
     1081 taaaaatggt aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt
     1141 aactgcaact gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa
     1201 caaaaaagac agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa
     1261 cagcaatcaa gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg
     1321 agagagtgac agtaatatct ttactattag cggtggttta atactgttag gaacgttagg
     1381 attattaggg tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct
     1441 tctctgagaa gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa
     1501 gtgttagtta aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa
     1561 aaatagtaat tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa
     1621 gcgattcaaa ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat
     1681 gtgggacatc gtta
//
LOCUS       DAYYHS010000194         1686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000194 DAYYHS010000000
VERSION     DAYYHS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.201"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..358
                     /locus_tag="ACHA6A_003018"
     CDS             158..358
                     /locus_tag="ACHA6A_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971125.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            355..627
                     /locus_tag="ACHA6A_003019"
     CDS             355..627
                     /locus_tag="ACHA6A_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8971126.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            628..813
                     /locus_tag="ACHA6A_003020"
     CDS             628..813
                     /locus_tag="ACHA6A_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971127.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            875..1147
                     /locus_tag="ACHA6A_003021"
     CDS             875..1147
                     /locus_tag="ACHA6A_003021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971128.1"
                     /translation="MSKDERKILANFYLVLKIEQNVFSERRIVWEKQNQRLRRKNDVW
                     NKRQSRMERLRKNKKATPFEIAAQYYSTIRGGVCEISLVKRLPRTR"
     gene            1107..1583
                     /locus_tag="ACHA6A_003022"
     CDS             1107..1583
                     /locus_tag="ACHA6A_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8971129.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
BASE COUNT          654 a          229 c          335 g          468 t
ORIGIN      
        1 aacgagtttt actaaattgt tgatattcat gataaatcac ctttttaaat ttaatttcga
       61 tttgattaat cgatacaagt attatatcaa ataaaaaagc tgatttaggg ggtcttacaa
      121 aaacactaaa attttttcaa aaagaggagg aacggtggtg aaagtcagag aacaattcaa
      181 gatagtgagc gacgaaggta atgtttacaa aataaagatc gttaatatta atgaatatcg
      241 agaagcagat cacaaatatt tggttgactt atacatgaac gataaatatt tgggagaagg
      301 attttgtgga gattcacttt ttgatgatga gcgaattttg aaggaggaaa cagaatgatc
      361 cacgaactaa aaatactacc agaatatttt gaagcagtga cgagcggacg caaacaattc
      421 gagatccgca agaatgatcg tgacttcaaa gttggtgatc agttaatact acgtgaatat
      481 aaaaaataca ttaaacatga attacctgga ctaacgagac caacatttag ttacactggt
      541 gactcataca aagcagagat cacttatatt actgattaca tgcagaagtc cggatatgta
      601 gtgttgggga ttaaaggggt gaattaaatg aaagtataca tcttagctat cacagagggc
      661 acatggatgt tcccagtcgg ttccggaaaa atctataaat caaaaacagc tgcttataaa
      721 gcatttgaaa aatacaaaaa agaaaacggt ggcggcacta acgcaaagat tctagtggct
      781 gataactggc atgaagaagg tgaacgtaat tgatttacct gttcattttc gtggtactaa
      841 ctatttttat gctagtaatc ttattcaaaa aaagatgagt aaagacgaac gaaaaattct
      901 ggccaacttt tatttagtat taaaaatcga acaaaatgtg ttttcggaaa ggagaattgt
      961 atgggaaaaa caaaatcaaa gattaagaag aaaaaacgac gtttggaaca aaaggcaatc
     1021 cagaatggaa cggctaagaa aaaataaaaa agctacccct ttcgagatag cagcgcaata
     1081 ctattctacc ataaggggtg gcgtttgtga gatttcattg gttaaaagat taccaagaac
     1141 tagatgagca gattctttac ttaaagtgga atcttaataa aagtaagctt gaattgaatc
     1201 gatgggtctg tggtgattta gcaaacgtcc gcatcgaaaa gaattcgaga acttcttctt
     1261 tagaagagaa tatccaaaag atagaaaatg aattggaatt gttgatcgag caaaaagaag
     1321 aaatgttatt gctgatagat agcttttctg gtatcgataa tcaaattgtt aagatgaaat
     1381 atgttgatca aatgagctta gaagatattg ctgaatctgt tgggtatagc tcatcgtatg
     1441 tcagacagcg acatgcagaa attagaaaga cattgaactt tttagatgag tatgaacaca
     1501 gacaagctga ccgattgaaa aaagaaaacg aaattgattt ttacaatagc gagaagtaca
     1561 aagaacaact ctctttattc taaaattaca ctgttcacta aatgttcgta aacattgtta
     1621 gcagcatgat attctattag tgtcaaaaaa atatgaaaga gccaagatat cccaaccgtt
     1681 ttatta
//
LOCUS       DAYYHS010000195         1653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000195 DAYYHS010000000
VERSION     DAYYHS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.202"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHA6A_003023"
     CDS             <1..156
                     /locus_tag="ACHA6A_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8971130.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACHA6A_003024"
     CDS             175..1452
                     /locus_tag="ACHA6A_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8971131.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..>1653
                     /locus_tag="ACHA6A_003025"
     CDS             1462..>1653
                     /locus_tag="ACHA6A_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8971132.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILF"
BASE COUNT          684 a          146 c          281 g          542 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg ttt
//
LOCUS       DAYYHS010000196         1653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000196 DAYYHS010000000
VERSION     DAYYHS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.203"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHA6A_003026"
     CDS             <1..1604
                     /locus_tag="ACHA6A_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8971133.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          606 a          269 c          339 g          439 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tgg
//
LOCUS       DAYYHS010000197         1604 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000197 DAYYHS010000000
VERSION     DAYYHS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.204"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1604
                     /locus_tag="ACHA6A_003027"
     CDS             <1..>1604
                     /locus_tag="ACHA6A_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8971134.1"
                     /translation="IDTFLNTVTIYAQSRTNRLGRRVVTLVEVDSKTGQEAISIIETK
                     MDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLLQYWGGEIKREPFKLSLLKRR
                     GRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQSEVGTTNRIYGSPVDSAYINN
                     YPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGCDKPKVSITVEFDKLTDSEEA
                     KEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSLSEKTLKLEAGDIQVAFYQQQ
                     AVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYVVLWPKEKPSNIFIMDNPDLE
                     KAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANFIQTGLIKADIFQNSFNKTGD
                     VLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTIGTTDSVGNPFPGASTPTPIE
                     DNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGG
                     GPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          587 a          203 c          325 g          489 t
ORIGIN      
        1 atagatactt ttttaaatac agtaactatt tatgctcaga gtcgtacaaa tcgacttgga
       61 agacgggtag tcactcttgt ggaagtggac tctaaaactg gtcaagaagc gatatcaatt
      121 attgaaacta aaatggataa aaaatctgac atacgacttt attctgatat tacggctgtt
      181 tctagcacaa tctttgaagc gagaaatgtt ttaaattgta ttgctggtga acaaggatca
      241 ttgcttcaat attggggcgg agaaattaaa cgtgaaccat ttaagctttc tttgttaaag
      301 cgaagaggtc gcaataatgt tggaacgatt cggtatggaa aagatatgtc tggtttgaag
      361 gtcaaattag attggacagg tataaaaaca agaattattc catatgctga tcctcaaagt
      421 gaagtaggta cgactaatcg aatttatggt tcgccagtag atagtgccta tattaataac
      481 tatcctgatg tctatacaga acatgttcag tttacagaag aacaaggagt aaaagacatt
      541 aagagcttaa ataaaatagc taaaaattac tttaaaacga tcaatcctgg ctgtgataag
      601 cctaaagttt ctattacggt tgaatttgat aagttgactg atagtgaaga agcgaaagaa
      661 tttgcgaaga ttagaaatta tggtctgttt gatacattta aaatatatca taaaaaatat
      721 aatatttatc ttgaatcaaa ggttagtgga ctacaatacg attctttatc tgaaaaaact
      781 ttgaaattag aagcaggaga cattcaagtt gctttttatc agcaacaagc tgtaactatt
      841 caggataaat taaaagatta tgcaacgaat aattatatga gtgattttaa tgattatgtt
      901 tcttcaatga ttacaggtca aggcagtgca ggaggatatg tagttttatg gccaaaagaa
      961 aaaccttcca acatctttat tatggataat ccagatttag aaaaggcaag agaagtacta
     1021 agaatgaata aaaatgggat tgctttttct aagaaaggat ggcgggggcc ttttaattca
     1081 gcttggacat tagatagtat atttaatgcc aattttatac aaactggatt aatcaaagca
     1141 gatatctttc aaaattcttt taacaagact ggagatgtat tgaaattagt taatggacta
     1201 ctccaaattt ggaacaacaa aaagaaaata atggagttaa ctaaaaaagg aatggaattt
     1261 tggaattcta agggttcaat tggaacgatt ggaacaactg attctgttgg taatcctttt
     1321 cctggggctt ctactccaac ccctattgaa gataattctt tagttattcg tacaaatgga
     1381 gacggcaaat atattttgat ttctcctaaa gtaggaaaag gattagtttt attaggaaat
     1441 ggtaaagcaa tttattttgg agacttagat gtacaaggaa aacttacagt taaaggaaaa
     1501 gaaataattc ctggacaagg tggtggtcca tcgggaggtg gagaatctac tggtgggtat
     1561 cctagtgaat taaaaacaga tgcagaaaag agagcttgga gaat
//
LOCUS       DAYYHS010000198         1593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000198 DAYYHS010000000
VERSION     DAYYHS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.205"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACHA6A_003028"
     CDS             144..542
                     /locus_tag="ACHA6A_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8971135.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="ACHA6A_003029"
                     /pseudo
     CDS             526..1220
                     /locus_tag="ACHA6A_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          606 a          232 c          311 g          444 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag cat
//
LOCUS       DAYYHS010000199         1583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000199 DAYYHS010000000
VERSION     DAYYHS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.206"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..>1583)
                     /locus_tag="ACHA6A_003030"
     CDS             complement(179..>1583)
                     /locus_tag="ACHA6A_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8971136.1"
                     /translation="EKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKIAFAWGGII
                     VLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLLITLIVIFP
                     ADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGFI
                     EHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSS
                     RGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKD
                     FLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIR
                     PTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFI
                     CIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIV
                     FWYYLGSISYLYLKKEN"
BASE COUNT          628 a          267 c          180 g          508 t
ORIGIN      
        1 cacaaaaaca acatcattaa atagccctaa ttcttttata cgctcactat atcttctagc
       61 gttttcaaat tcagaataaa taaataaatc tccttttgat tctcggtaaa aattttgata
      121 aacatttaat atattaatca aatgtaaagg agtccaagca atgaaagctg tcttcatatt
      181 aattctcctt ttttaaatac aagtaagaaa tgcttcccaa ataataccag aaaacaattg
      241 aaatgacatc gcgcttattg aaaagtacat gagcctccac catttcatct ataaaaatag
      301 atagtataaa aattaaaaca ataccaagaa acgcataatt cactttactt ctaaaaaatg
      361 ttttgagtaa actaaagata tttaacacat agaaagcata gatacatata aaagcaatta
      421 taccacttga tacaagtact tgaagaaatc cattatgcat gtttccatgt gctcttttta
      481 gttcattcac atctgtagta tcaagatcta cttttttcaa aaattttgca tcgctattcc
      541 catagaaatc ggtctctcca taaccaaata acaaagttgg tcttataact ttaagcgcag
      601 ctttccaaat cgtaaaacga ccagctgaaa tttcggagtt atcactagaa tgctgtaata
      661 ttacttcttt ttctggaagt ggagttttat ctgattcatt tgactctata acttctactt
      721 ttctatcaat cgatacagga attgttgcta aaaaatcctt agcataatta gtaccaaaag
      781 aaaaatatac caatagaact aataatatgc ttaccatcca tttgattttt acaggtagta
      841 cttctctaat gctctttcta aattttgaat tggcaaacaa caaaagaaat aatactaaaa
      901 aagaacaaat tactacctgg gcacctctag acgaagaaag tgtaatataa aatatttgta
      961 cgataaaatt catcatataa aaaacacgaa tcgtttttgt tatttttgaa attaatacac
     1021 taattaaact taaaataata gaagtatatc caaacatagc tccaacattt ggacttgtat
     1081 agacaccaaa taaccgatgt tcaataaaac cttgtcttgc aagtaatcct ggcgttcctg
     1141 ttgggacatg ataagaaatc aaaaaaataa attgatagat agatattagc gcagctaaaa
     1201 aaattatgat aatgaaaaca ttattaaaat acaccatttt tttctttttt tcctcttcac
     1261 tttgtgaaaa gtctgcagga aaaattacta tcaaagtaat cagcaaatat ccaaaattat
     1321 aaatagatgg aattaaaaaa ttttctctgt taatgattat agttaagata aaactaatca
     1381 ttgctccaat cagtagccaa gaaaaacgca ttttaaaaaa atttttcttt ataaaaacct
     1441 catacaaaac tataattcca ccccaagcaa aagctatttt catcgggata ttagttacat
     1501 taaaagtact aggcataaaa taaagcagac aaatgattaa atttattact cgaaacgcat
     1561 ctcgacttaa taaactcttc tct
//
LOCUS       DAYYHS010000200         1545 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000200 DAYYHS010000000
VERSION     DAYYHS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.207"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            976..1290
                     /locus_tag="ACHA6A_003031"
     CDS             976..1290
                     /locus_tag="ACHA6A_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971137.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(1343..>1545)
                     /locus_tag="ACHA6A_003032"
     CDS             complement(1343..>1545)
                     /locus_tag="ACHA6A_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971138.1"
                     /translation="AGYVDYEYTADYLETPLQHDNNDINFRFIYNSKSKTWLVDQLST
                     INTGFHELPKPDNETALVIKTF"
BASE COUNT          608 a          194 c          258 g          485 t
ORIGIN      
        1 cgagatccaa tttttttaag gaagtttaac aaaaatatgt aattctatta tcactattaa
       61 agagaaagtt ggagcggaat ataaagaaaa gagaaaagta attaaacgca aaaaaatgta
      121 gaacttaaaa aatataaaga ggaacaaccg ttaaaattag gaatgatgtt gtgtaaacat
      181 tataattgct atgatataaa tgaagtgtta aagaaagcaa atttaaacca attttgtgaa
      241 cagaaaagcc ttagtcacta tgaccaaaac tacggacgaa actatgacca aaagtaggta
      301 ctaaattatg accgaaaata ggtccaaaat ggattttttt atgatatcaa aaattatgaa
      361 atgattttga aagaaaacca gttggcacag ccaacttagc caccttcctg taggaaggtg
      421 ctatcagttt tcccttatgc tcttttcttt cttttttgtt tcatatgtca aatgccattt
      481 tgaaatgtac catcttaaga aatttatcct taaaaacaat tgcgttattg gataaaaaga
      541 tgctacatta tcttaaaaaa ggaatcaaat atgatgaatt tagtggctag catatcgtgt
      601 gattaagata ctttatagtg taagttgaag gagaaaaaat atttaaaata tacttcccat
      661 ttcagtggga agtaaaatta atcttgttgt ttctactagt aatgtatctt tttggaaact
      721 atgtaacgat gtaaacaaga tttttttcag aagaaaaaac gataaaagtt gtgataaacg
      781 atttaaaaaa taggagaaaa tatatcaagc gcatggtatg aaacgcacat ttaaaaagat
      841 tcgactaact aaaaaaataa gtttacaaag cattgttgtg tattacaagt gatctataca
      901 ctgaaaaatg atagagaaaa tctgatacgt ccgcaacaat ggacaattga aaatgaaagt
      961 gaggaaattg gaagaatggc tgatgaaaat aaagttgttc cgcttgaaac actagtgata
     1021 aataaggaag agtttgagca actttttctt gatttttttg ttcaatatga caggatttat
     1081 gattttagtt cgtcactaga tttatttgaa ttagaggcag aaataataga aaacagtcga
     1141 gaggagaatg gtaaaatcat catttcagta gaaaaattca atgaattatt ctcagaaaca
     1201 tttgaaatag atacagatta ttatcaacca caagaagttt tccaatcatt tagaaatcag
     1261 attattcgct caggtaagac cgtaaactga aaaacaataa aagaaacgtt gaaagaataa
     1321 ttattccttc aacgtttctt ttttagaatg ttttaatgac caaagcagtt tcattgtcag
     1381 gcttaggtaa ttcatgaaat cccgtattta tagtgctcaa ttgatcaact aaccatgttt
     1441 tactttttga attgtagata aatctaaaat taatatcgtt attatcgtgt tgaaggggcg
     1501 tctccaaata atctgctgta tattcatagt caacgtaacc tgcta
//
LOCUS       DAYYHS010000201         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000201 DAYYHS010000000
VERSION     DAYYHS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.208"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHA6A_003033"
     CDS             64..972
                     /locus_tag="ACHA6A_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8971139.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHA6A_003034"
     CDS             972..>1480
                     /locus_tag="ACHA6A_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971140.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAYYHS010000202         1455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000202 DAYYHS010000000
VERSION     DAYYHS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.209"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1029
                     /locus_tag="ACHA6A_003035"
     CDS             <1..1029
                     /locus_tag="ACHA6A_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8971141.1"
                     /translation="MMNLYRKIDRTNYQFDFLIYEDYADVQDYHSEIERLGGRIFVVP
                     NPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDG
                     KSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNS
                     EAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSL
                     RNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLK
                     LVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQM
                     ESIYSRDH"
     gene            1062..>1455
                     /locus_tag="ACHA6A_003036"
     CDS             1062..>1455
                     /locus_tag="ACHA6A_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8971142.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKE"
BASE COUNT          566 a          153 c          260 g          476 t
ORIGIN      
        1 atgatgaatt tatatcgtaa aattgatcgt acaaactatc aatttgattt tttgatttat
       61 gaagattatg cagatgttca agactatcat tcagagattg aaagattagg aggccgtatt
      121 tttgttgtac ctaatcctaa gaaaaatatt tttaaatatt taatagaagt gaataagtta
      181 ctaaaaaaag aatctttttc tatagttcac aatcaggttt attttggcgg gggaattaat
      241 ttatggttag ccaaaaagaa tggaatcaga cagagaattg ctcatagcca tgcaactgaa
      301 gatggaaaat ctcaaaatat tgtaatgaat gtattgcgta aatttttaac caaattgttg
      361 ttacaaaatg caacagacta ccttgccgtt agtcaagaag ctggtgaaag tctgtttcaa
      421 aaccacccct ttgagatagt tcataatggt attgatttag agctttatag taaaaattca
      481 gaagctaagg ttaataagag aaaagaattg gatatctcaa tgagtacttt tgttgtggga
      541 aacattggac gattagagaa acaaaaaaat caatcttatt tgttagagat ttttttagaa
      601 atactaaaaa tgaattctga ttcttacttg attttagtag gtgaagggtc acttagaaat
      661 aaactgaaag ccaaagcagt agaattggat atagagaaaa gagtaaaatt tttaggagaa
      721 agaaacgata ttcccgagtt attatctgtg atggatgttt ttgtaatgcc ttctctttat
      781 gaaggtctgc caatggttgg attagaagca caagcagcaa aattaaaatt agtcttagca
      841 gatactattt ccaaagatac aaagttaaca aataatgtgg attttgtatc attagatgaa
      901 acaccacaag aatgggcaaa aaaagtaata aaagagccgt atgctaataa aataacacaa
      961 gaactaaaga gttatgatgt ttcgtacact actgaacaaa tggagagtat atatagtaga
     1021 gatcattaaa aatgttcaag atattttagg agtgattatt tttgaaaata tgttatttga
     1081 tattggcata caatgatatt ggcaacttaa agagattggt caatcgattg aacgaaaatg
     1141 cagtttttat tattcatata gataaaaaaa ctgatataga accatttatt gaagcattta
     1201 aatcatatga taatgttgaa tttttatgta ataaaaaccg tgttaaaatt tattggggtg
     1261 gtttttccat tgtacaagca gaaatcaatt tagtaaagag agcattacaa aatgagaaat
     1321 atttaaaata tgtattatta tctggtgctg attatccaat caaagataat gagtatattt
     1381 ataattattt taaaaagaat agttcagtgg agtttattcg tggaatagat ctagatcaaa
     1441 ttaaacataa agaat
//
LOCUS       DAYYHS010000203         1422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000203 DAYYHS010000000
VERSION     DAYYHS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.210"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..384
                     /locus_tag="ACHA6A_003037"
     CDS             <1..384
                     /locus_tag="ACHA6A_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8971143.1"
                     /translation="EELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENEVTIVNKS
                     RYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFKW
                     LEFLFPKFLEDSSELQDIYNYLYRR"
     gene            389..1336
                     /locus_tag="ACHA6A_003038"
     CDS             389..1336
                     /locus_tag="ACHA6A_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8971144.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          480 a          201 c          287 g          454 t
ORIGIN      
        1 gaagaattaa tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt
       61 tctgcaagac gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata
      121 gttaataaat caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc
      181 tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa
      241 tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga agaagatttt
      301 aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc
      361 tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg
      421 cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga
      481 tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga
      541 gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta
      601 tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac
      661 acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc
      721 tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt
      781 accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc
      841 tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg
      901 ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc
      961 tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt
     1021 tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact
     1081 tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc
     1141 aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact
     1201 tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat
     1261 ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa
     1321 aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc
     1381 aataaggact gatgagtttt gtaaaatcaa atctgggata ga
//
LOCUS       DAYYHS010000204         1421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000204 DAYYHS010000000
VERSION     DAYYHS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.211"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHA6A_003039"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHA6A_003039"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8971145.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          496 a          184 c          236 g          505 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatacta t
//
LOCUS       DAYYHS010000205         1411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000205 DAYYHS010000000
VERSION     DAYYHS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.212"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..443)
                     /locus_tag="ACHA6A_003040"
     CDS             complement(144..443)
                     /locus_tag="ACHA6A_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8971146.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            794..>1411
                     /locus_tag="ACHA6A_003041"
     CDS             794..>1411
                     /locus_tag="ACHA6A_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8971147.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQ"
BASE COUNT          478 a          226 c          199 g          508 t
ORIGIN      
        1 attaaaggat aaaatacaat ataaagaaac atctgtttct ttatttagaa taaaaaaata
       61 cagactctgt tcaaataaat gaacctagcc tgcattatga gattgactat actttttaat
      121 tcattgagat gaataactcg tttttataaa tgcccaaaag taatcggtgc cgaagccatt
      181 cctcgaccta attctttttc aaattttcct attgttgata tctccatata aaacggtgat
      241 actggtttag acaattctcc cctgattgct aacggacgta aactacgttc caaattattt
      301 atgactttag gataatactg acctttttct aagcctgtct ttgcgtctat caaaagtata
      361 cgttcctttt ctttgatctc tggatttaaa acgagatcat ataattttag taccatgttt
      421 tctatttctt cacttttaga catcacgatt tcctcccaaa aatatatttc ttcccactac
      481 attctataaa caaagtatac gctacatcta gattttttta aatcattact ggattgttaa
      541 aacctttcaa tacactgtta tggcaacgat ttttatttta aaaacattct aaattaaatc
      601 agtaaaaatt tttattctaa gatttttatt agaattacgc ctaatttgtg tctctcaggg
      661 caattctaag aacgtttcaa gagatagcga cactatcgtt atcgaaaaag gcgaaaacct
      721 atctcatttt ctgctaatgt aagttggttt agcttttgtt aatatgactt tatgaaaatg
      781 gaggtaatta aatatgattt ttcctaaagt taatgaagca agcttgaata aaggatcctt
      841 ttttcatatc tatgaaaacg aagattttga actgtataat atcctttcac aaaaagaggt
      901 catcgaaaac caaataatct gccatccaat tatttacaaa tctattttca agtatgcatt
      961 ttttgcttcc aatgattttc aaagaatgga gattaccgac tgtatattcg aaaactgtga
     1021 tttttcaggc tgtatattga caaaaagcat gttgcatcgc gtgaaatttg tcaattgtaa
     1081 attaacaggt actaaattga tggaaagtta tattgggaac acgttattcg agaattgtaa
     1141 aatggactat gtaaatttat ctggttcaaa cataaaagaa agtacctttg agcactctgt
     1201 cttatcaagc gcagattttg tagactgttc cctcactaaa ataatgtttt ctactaatga
     1261 tttagaaaat gctagctttt ttgatactga tttaaaaaat atcgatttaa gtagcaactc
     1321 ctttgaaatg attgaagtac gctcggaaca tctaaaaaat tgtacgctta atcagttgca
     1381 agcacttggt tttgctagaa aatttttaca a
//
LOCUS       DAYYHS010000206         1391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000206 DAYYHS010000000
VERSION     DAYYHS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.213"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..1044
                     /gene="scrK"
                     /locus_tag="ACHA6A_003042"
     CDS             172..1044
                     /gene="scrK"
                     /locus_tag="ACHA6A_003042"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8971148.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          443 a          237 c          314 g          397 t
ORIGIN      
        1 ttcatatatc aattgtatga aaaattatta gaattttaaa aaatgataaa aattcaaaaa
       61 gaaaaaatag tagttattta cttgtcatgt tactaaataa aaaaataatt taaatatgat
      121 atatagtcat ttatttagtg atttcattct aaaataaagg gagaaatgtc tatgctatac
      181 ggtgcaattg aagcaggcgg cacaaaattt gtgtgtgcca ttggcgatga agaaatgacg
      241 atcaaggaaa gagtgagctt tccgacaacg acacctgagg aaacaatgcc tttagtcatt
      301 gacttcttca aacaatacca agcagattta gcagggattg ggattggctc atttgggcct
      361 atcgatatcc atcgcgattc cgccacttat gggtacatta cctcgacccc gaaattggca
      421 tggcaaaatt ttgatttcat cggtaccatg aaaaaagaat ttccgatccc gatttcttgg
      481 acaacagatg tcaatgcggc tgcctatggt gagtatgtct tcggcagtgg caaaggattg
      541 agcagtgtcg tttactatac gatcggtacg ggtgttggcg gcggtgcctt acaagaaggg
      601 cgctttatcg aagggtttag tcatccagaa atggggcata tgctcgtcgt ccctcatcca
      661 aaagatgact ttgctggcag ctgtccattt catggcaact gtttagaagg gatggccgct
      721 ggacccgcca tcgaaaaccg cttagggaaa aaaggacaag aggttgcaga agatgatcct
      781 tattgggaaa tcgaagcctc ctatattgct caatgtgcct acaatacaac cttgatgttt
      841 tctccagatg tgatcatttt tggcggtggt gtgatgaaac aacgccatat gctgcaaaac
      901 gtccacgacg catttgcaaa attggtcaat gggtatgtcg aaacacctga gttaagcaaa
      961 tatatcgtga ccccagcatt agaagacaac gctggaacgc tcggttgttt ggcattggct
     1021 cgagaagcgg tcttacattc ataaacgaaa aaaggctctt cgacatttag tgttggtaga
     1081 gaaagataca actgaaaatt tagtcataac cagttaggca gagtgcttaa ctggtttttg
     1141 ttttacttgg aagatcaagc cttgaatgag ataaattctt gctcgaaagt tatggaaatt
     1201 tcgatagccg taggccactt gtattgtgca atagtttttt acaaaaaaaa gggggtgcgg
     1261 atattttttt ggaccttttt aacaaatagg ttctgttgca aagttttaaa tctactatca
     1321 gataaggtag aataatagaa aaagatagca ggaggaatga cgatgagtca ttttaaagga
     1381 aagcaatttc a
//
LOCUS       DAYYHS010000207         1362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000207 DAYYHS010000000
VERSION     DAYYHS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.214"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACHA6A_003043"
     CDS             82..>1362
                     /locus_tag="ACHA6A_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8971149.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAYYHS010000208         1357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000208 DAYYHS010000000
VERSION     DAYYHS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.215"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..382
                     /gene="spx"
                     /locus_tag="ACHA6A_003044"
     CDS             <1..382
                     /gene="spx"
                     /locus_tag="ACHA6A_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFM8971150.1"
                     /translation="TSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEGGVQ
                     DIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDEIRS
                     FVPREIRCVARNLIYLQNNNILNS"
     gene            396..752
                     /locus_tag="ACHA6A_003045"
     CDS             396..752
                     /locus_tag="ACHA6A_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8971151.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          497 a          202 c          253 g          405 t
ORIGIN      
        1 tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat caacttgaat ttgaagagaa
       61 gaatattttt gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga
      121 aggaggagtt caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga
      181 ttttaacgaa ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt
      241 gagacgacct attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat
      301 ccggtcattt gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa
      361 caataacatc ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag
      421 cgcactgtgt aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc
      481 aatttgttga cttagaagaa cgcgatttaa gtacagaaga agcagaagaa ttatgcagtt
      541 ttgaagaagt gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg
      601 cgattgacct ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca
      661 atctcctcag acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc
      721 agttgatgga acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga
      781 gcatttcata cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt
      841 ttgactttat ttttctacta tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta
      901 ggtggggata tagagttaaa aatatattga catgtgtaaa tttacatgat atattcattt
      961 ctgttgtttt aagtagcaaa gcaataaaat agtattttaa aaaacatata aaatagttgt
     1021 tgacatgtta gaaatgacat gataagataa ctgagttgtt aaaaaagaac cacttgtgaa
     1081 gcttgattct atcgaaaaaa acttcaaaaa agttcttgac aaataacaaa tgataagtta
     1141 tgataaatga gttgctgtaa gatttcgaaa aacgaaataa aaaaaacttt taaaaaagag
     1201 ttgacaatta cttgtctctc tgatatgata taaaagttgc tacggcagcc aaaaacgaat
     1261 cggggaacac tcctgatgaa gtagaccttt gaaaactgaa caaagtaaga caaaccaaat
     1321 gtgtagggcg tcttgattca attcaagaca acaaaca
//
LOCUS       DAYYHS010000209         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000209 DAYYHS010000000
VERSION     DAYYHS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.216"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHA6A_003046"
     rRNA            <1..1355
                     /locus_tag="ACHA6A_003046"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYHS010000210         1346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000210 DAYYHS010000000
VERSION     DAYYHS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.217"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHA6A_003047"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHA6A_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHA6A_003048"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHA6A_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          481 a          218 c          180 g          467 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt ttttta
//
LOCUS       DAYYHS010000211         1324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000211 DAYYHS010000000
VERSION     DAYYHS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.218"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHA6A_003049"
     CDS             278..820
                     /locus_tag="ACHA6A_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971152.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACHA6A_003050"
     CDS             976..>1324
                     /locus_tag="ACHA6A_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8971153.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAYYHS010000212         1232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000212 DAYYHS010000000
VERSION     DAYYHS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.219"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..879
                     /locus_tag="ACHA6A_003051"
     CDS             <1..879
                     /locus_tag="ACHA6A_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM8971154.1"
                     /translation="LILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIK
                     IFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKV
                     RREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVI
                     RENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDV
                     HAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTK
                     LAKAEQYRRSLELSKD"
     gene            951..>1232
                     /locus_tag="ACHA6A_003052"
     CDS             951..>1232
                     /locus_tag="ACHA6A_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971155.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSL"
BASE COUNT          543 a          144 c          201 g          344 t
ORIGIN      
        1 cttattttaa ataaacgaga gaaaaataat ttaaagaaaa aaatgctcat tccatttgat
       61 tcagaagtga aaggattact caataaacta actatttcta atatagactc gactagggaa
      121 aaaatcataa aaatatttga taaaattaat aatttattca agaatgacaa tgctgaatta
      181 aggaagggtc ttttagagtt taaacttcaa gaactagaac ttcattactc ttatttagtg
      241 aaaattaccg aagaaaaaga acaacaaaaa gcaataaaag aacagatgat agaagaggaa
      301 aaagttcgta gagaaattga tcgtgaaaag aaacgtattg ataaagaaca acgtcaattc
      361 aattctgaaa tttccaaatt aatcgcatat atgcaaaaag ctaatgcaga tgtggaaaaa
      421 gaattatatg caaataaaat tcaagaactg gaagaaaaat taaaagaatt agaagttata
      481 cgtgaaaatg tattacaaag agaattaaat actagagccg gttatgtgta tgtaattagt
      541 aatattgggt cttttggtga agacatatat aaaattggta tgactagaag attagaacct
      601 atggatagaa taaaagaatt gagtagtgca tcggttccat ttgaatttga cgttcatgcg
      661 atgatttttt cagaggacgc tccgtctttg gaaactaaat tgcataatca ttttagaaaa
      721 caagaagtta ataaaattaa tcaaagaaaa gaatttttca aagtttcact agacgaaata
      781 gagaaagttg ttttagaaaa ttataatgga acagtaacat ttacaaaatt agctaaagct
      841 gaacaatata gacgatcttt agaattgtca aaagattaaa tatataaaaa gccttcgggc
      901 tttttctttt aacaattaat aggaacgtat attcgaaagg aatagaaata atgtggttag
      961 aaaaaataaa tgacaaaaaa tttaagtaca ccgaacgtta tacggatccc ttaactgaaa
     1021 aaaagagaaa agtgtccgtt actttatcta gcaattcacg ccaagcttgg aatcaagcta
     1081 acttactttt gaatgaaaaa atcgcagaaa aaataaaacg aaatgaagaa ttaccactaa
     1141 cttttggaga attaaaaact aaatgggatg aaaaatacaa gccaactgta aaagaaagtt
     1201 cctatcgaac aactcaggtt tatctatcat ta
//
LOCUS       DAYYHS010000213         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000213 DAYYHS010000000
VERSION     DAYYHS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.220"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHA6A_003053"
     CDS             414..947
                     /locus_tag="ACHA6A_003053"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8971156.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYHS010000214         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000214 DAYYHS010000000
VERSION     DAYYHS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.221"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHA6A_003054"
     CDS             <1..1076
                     /locus_tag="ACHA6A_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8971157.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAYYHS010000215         1192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000215 DAYYHS010000000
VERSION     DAYYHS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.222"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1125
                     /locus_tag="ACHA6A_003055"
     CDS             85..1125
                     /locus_tag="ACHA6A_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8971158.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          483 a          174 c          197 g          338 t
ORIGIN      
        1 tgcatttttt atttatcttt gctattttcg aaaagagtag gtaattttac ataaataaaa
       61 caacgtgaaa gtagaggatc tacaatgact gattttaaat tttttaaggc ggatagagtt
      121 tacaacgaat tattttatca atttccaaaa gtctttattg tttctgacga atacaaaaaa
      181 atgaaagatt caactaagat tgcctatatg cttttaaaag caagattaga gatcgcaatc
      241 agcaaacggc aaatcgatga agaaggtaat gtttatttta cttatacgac aaatgaacta
      301 tgtagagtat taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga gttggaatcc
      361 tttggtttat tattacaaaa gcagatggga tttaacaaac agttagggaa aaataatcct
      421 aatagactat atctagcaga attaaaagtc tcagaaaatg atatctactt actcgaaaaa
      481 tttgatagag agaataggga aaacgttgat aaatcagagg gtatgaaaat catacccacc
      541 ctcgacgaaa aatcagacgc tgaatccctt ggggctcaag agggtatgaa aatcataccg
      601 tgccaaaacg ttgataaatc agagggtatg aaaatcatac cagaacttaa taataatata
      661 ttagacacta atagacacaa tatagacact gaaaaagacc gcctacaaga tcaattgttg
      721 ttagacaatt ttgagacaat tatgacaaac gacagcattg ctacgtttgt ccctgaacga
      781 gtattaaatt tgataaaaac attttcttca agttacagtg aagctcaaaa aaccgtccag
      841 actattcata atgcaaagaa aaaagctgaa atagaaagtg atatttcgat agtttttgaa
      901 gaactcgata gttattatgt caatgcagaa caagaattat acacgacact gttaaaagcc
      961 tatcaaaaat taaaaaccga aaaagtcgaa aatatccaga acctgatttt tgtctatgta
     1021 aaaaattggt ttatcgaaaa accaatagct gctaaagtat caagtgaaaa acgtttgaat
     1081 tatgcaagct ccccaagcac tattacgaaa gactggttag agtgaaactt tactgacaaa
     1141 gtgattgttg cttcatggag taacattaaa attaaaaatt ttttattatt ct
//
LOCUS       DAYYHS010000216         1110 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000216 DAYYHS010000000
VERSION     DAYYHS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.223"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(265..969)
                     /locus_tag="ACHA6A_003056"
     CDS             complement(265..969)
                     /locus_tag="ACHA6A_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971159.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          193 c          199 g          397 t
ORIGIN      
        1 tttttctcta tcagaaaata tgtttattat tagaattttt tatagttaag gttaggcatc
       61 ttaagaaatc aggcaactcc aagatatctt caggtagaag agaagtcgcc taacattgta
      121 ggatcatagg tgatttgttg gacaggtcaa gctgatattt gagcctgatg ttagaaggaa
      181 tttcttttta cttgctgtag aaattattat atgactaaaa aggctgggat tttaaatccc
      241 agccttttat gacaaataga atttttatcg atcagtaatc gctgtcgctg caaattgttc
      301 gatcaaagga atcaaagtca atttgacttt gtatttccgt tcgagtgctt tttcgataac
      361 gaactcattt tcagtagtca tagaaatagt tgctaattta cgaggttcat cagcattttt
      421 ttcattaata actacttcat gttcattttg ttctgtgtaa aatttaggat actcagcaac
      481 ggcttttgaa aaatcagcaa aacttaaagc ggcattcgta tcagttgagc gtgtattttc
      541 agttgtgtcc gcttttggtt tttccgtttt ttcttctggc acactgactg tttcttctgt
      601 aactggtgtc gcaacagctt gcgcagttgg ttcatcgttt ttcaatcgtt gatcattcca
      661 gtaagacacg atttcaataa gaagattgtc acggaatcgt tcgaaaatcc cgttttctcc
      721 tgaacgaatt ctctttaata cagtattgaa gttgtcatcg attggcacgt tgatccgtgt
      781 atcttcattt gttttcccag ctaatccgat cgttaagatt tgacggtttt tgggatcaag
      841 catgacttgt aacggcaaac cataacgtcg caagctctct tcttctagag aacggaataa
      901 ttgtcctctt acaccatcca atgaaggacg gaattgattt aataattttt gtttctgttt
      961 tttcttcata tgttctctcc ttcgctcata ccctttcgat tataagaaaa aaacagccaa
     1021 atgaaaagga gtaatgcaaa aaaacttgcg tatacgcaaa ttacaacaga aaaatttgcg
     1081 tattttcccg ttttttgaaa gaaaaataaa
//
LOCUS       DAYYHS010000217         1022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000217 DAYYHS010000000
VERSION     DAYYHS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.224"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..461
                     /locus_tag="ACHA6A_003057"
     CDS             3..461
                     /locus_tag="ACHA6A_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8971160.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            470..697
                     /locus_tag="ACHA6A_003058"
     CDS             470..697
                     /locus_tag="ACHA6A_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8971161.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          167 c          180 g          356 t
ORIGIN      
        1 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
       61 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
      121 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
      181 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
      241 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
      301 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
      361 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
      421 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
      481 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
      541 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
      601 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
      661 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
      721 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
      781 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
      841 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
      901 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
      961 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1021 gt
//
LOCUS       DAYYHS010000218         1016 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000218 DAYYHS010000000
VERSION     DAYYHS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.225"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..227)
                     /locus_tag="ACHA6A_003059"
     CDS             complement(<1..227)
                     /locus_tag="ACHA6A_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8971162.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMF"
     gene            903..>1016
                     /locus_tag="ACHA6A_003060"
     CDS             903..>1016
                     /locus_tag="ACHA6A_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8971163.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQE"
BASE COUNT          374 a          157 c          127 g          358 t
ORIGIN      
        1 ttaaacatgt atagaagaaa catctcatta aaatcaccag taaagaaatg gaaatcttta
       61 gtttttttaa tattatcagt tagtaacttt ttataatcat aaaaaaaata agtagttcta
      121 tctgagcgat actcttttat aatactgaat tcggaattaa aaaccacgat aggtaacttt
      181 aaacaaacat gaagagactt taataagact tctttatctg aaaacacttt tgtttctctc
      241 tttcctttct acatcattaa atagtatttt atacgttatc taaaatacta ttcctctgtc
      301 aaaataagct aacttacttc attataaaat atccacttat cgctttcatg tatattccac
      361 cgtaaaaagt acattttatc accgagaact catcattttt catcatgatg aatactattt
      421 acatatatct agttcaaaga caataattat tatgatccgt tattatctaa gcactataca
      481 gataatagga atttttcgtt tctaaatatt ccaatttggg aatccaataa aaattttcgt
      541 tcaaatacgt gtctattcga actcttttct agttgttaat tatgaagggg gaagaaataa
      601 aaaatacaat ctatacacga ataagaatat tatccaaaag gaggttttag aaaagattta
      661 ttatacgtaa aaagctcaag acattttctt agccataagg acatgtttga tatttaagaa
      721 gatatatgaa ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca
      781 tttttgaaaa taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc
      841 agcgcagcat aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct
      901 taatggagga ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc
      961 gattctttga aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaa
//
LOCUS       DAYYHS010000219         1005 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000219 DAYYHS010000000
VERSION     DAYYHS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.226"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1005
                     /locus_tag="ACHA6A_003061"
     CDS             <1..>1005
                     /locus_tag="ACHA6A_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971164.1"
                     /translation="LYLFPAFKNMEIFGFYDTAFHINRALSLESIFSSPINFETFRSY
                     GMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTS
                     SFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMT
                     LLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMI
                     EQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKK
                     LGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFI
                     S"
BASE COUNT          315 a          144 c          134 g          412 t
ORIGIN      
        1 attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg atacggcatt
       61 tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta attttgaaac
      121 gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc tatatccttt
      181 atttttattg atcaagttta ctaatttagc aataggctat aacctttttc tttatattgt
      241 tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca aaaaacatgt
      301 tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg tagaaatatt
      361 tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta ttttactcgg
      421 gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga ttagcatgac
      481 tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga ttataattgc
      541 tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta ttaaattaat
      601 aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta tgattgagca
      661 gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa gagcaattta
      721 tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat ttggtattgg
      781 cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat taggaatctt
      841 atctaaaatc acttatataa tgggtataat atctattcta ctttgtacaa aaatatttcc
      901 atggtttgcc ttacaaaata ctcctttaaa tattattcaa gctccttggc gttttatgat
      961 attttcaaca ttgttcatat ctttttcagt atctctattt atttc
//
LOCUS       DAYYHS010000220         1000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000220 DAYYHS010000000
VERSION     DAYYHS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.227"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1000
                     /locus_tag="ACHA6A_003062"
                     /pseudo
     CDS             369..1000
                     /locus_tag="ACHA6A_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DAYYHS010000221          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000221 DAYYHS010000000
VERSION     DAYYHS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.228"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHA6A_003063"
     CDS             <1..>986
                     /locus_tag="ACHA6A_003063"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8971165.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYHS010000222          956 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000222 DAYYHS010000000
VERSION     DAYYHS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.229"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHA6A_003064"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHA6A_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8971166.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          282 a          196 c          141 g          337 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaattat acagaaacac tttattcaaa ataggaaaga
      841 tcaggaaagc catcaattgg aggaaaaaac tctgtttgtt catcatgata tagattatct
      901 tttgctttaa aaaaaaccaa ttatgaattg acaaataaaa cctgttttca tataat
//
LOCUS       DAYYHS010000223          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000223 DAYYHS010000000
VERSION     DAYYHS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.230"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHA6A_003065"
     rRNA            <1..>943
                     /locus_tag="ACHA6A_003065"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYHS010000224          922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000224 DAYYHS010000000
VERSION     DAYYHS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.231"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..579
                     /locus_tag="ACHA6A_003066"
     CDS             <1..579
                     /locus_tag="ACHA6A_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8971167.1"
                     /translation="EKINNNLTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEV
                     SKQKVASLENDKNQLVSEKTNLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLN
                     SDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSS
                     LNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          412 a          112 c          126 g          272 t
ORIGIN      
        1 gaaaagatca acaacaattt aactatatta gacacagctc tagaaaataa agagcaaaaa
       61 attagtattt taaattcaca attgtcttca aacaaccaaa ctcttgaaca actaagaact
      121 aatattgaag tttccaaaca aaaagtggca tctttagaaa acgacaagaa tcaattagta
      181 tcagagaaaa ctaatttaga aaatcaattg tcagcaaaag actctgaatt gcaaggaaaa
      241 caaaatgaaa ttaatgcgaa aatagacgaa attaaccaaa aagtagctga aataaatcga
      301 ttaaatagcg attggaataa tcgtttacaa aatgaaaaga acaaatttaa tgaagaacaa
      361 tctactatta atagtttaaa ccagcaaatt aatcaaatgt tgagtgaaaa aaataacatt
      421 caaaatcaac tgcaagctac tcagcaaaag ctagaagaag aaaagtcaaa aaatagtagt
      481 ttgaataatt acattgaaaa gcttcaaaaa gctaaaaatg atgtagagga taccgcaaat
      541 aaagcacaac aaattgtaaa tgaacataca atccaataga tatagtttta atttaacaaa
      601 tggtatgttt ggagataatg agatagaata taaatgtcat aatattatac aatctaaaca
      661 ccgtttcctt ttctattttt gatgacatca gtatatagct actcgaaaga gtagtctttt
      721 tattattctc gcttattatt gatatgaaaa tttcgatttg acaaaaggta ccgaataatt
      781 ttgtaatatg aattattatc aagaagtaat tatgatgata acatatggaa actagttagt
      841 aataataaca ggtgattaaa cttttgaacg acttgtaatt ctatattttt tcttttaagt
      901 aaaaaatata ggtgattttt tt
//
LOCUS       DAYYHS010000225          901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000225 DAYYHS010000000
VERSION     DAYYHS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.232"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..>901
                     /locus_tag="ACHA6A_003067"
     CDS             72..>901
                     /locus_tag="ACHA6A_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8971168.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          316 a          134 c          164 g          287 t
ORIGIN      
        1 aatagaaaaa aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag
       61 aggacttcca tatgaacgat tttactacag aaattatgga aatactaatc aataaaggtg
      121 atttggatga tttgtttcgt cgtcacctag aattggctat taacgcttta ttacaagctg
      181 aattaacggt atttcttgac tatgaaaagt atgatagagc tggatttaat tcaggtaatt
      241 cccgtaatgg gaattattcg cgctctttta aaacagagta cggtgaattg aatttagtaa
      301 tacctagaga taggaatgga gacttttctc aacaaacttt acctgcttat aaaagaacca
      361 atgactcttt agaaaccact attattcagc tagttcaaaa aggaattact atgtccgaaa
      421 tctctgatct aatcgaaaaa atgtatggtc attattacaa accacaaact atttcaaata
      481 tgagtaaaat tgtgtctgaa gatgtttcgg catttaaaga aagaacttta gaagctaaat
      541 actcagtcat ttttatggat gctactcata ttcccttaaa aagacaaacc gtatcaaaag
      601 aagctgtcta tatagtaatt ggtatcaggt tggatggaac taaagaggtt ctaggattta
      661 ctatagctcc aactgaatct gcttatgttt ggaaagagat tcttcaagat ctaaaagacc
      721 gtggtttaga agaggttttg ttagtcgtaa cagatggttt aagtggtatt gacgatagta
      781 ttcatagtgt ttatccaaat gctcaatttc aacaatgttg tgtccatata tctagaaata
      841 tcgctcataa ggttcgtgtt agtgatcgac aagaaatctg tagcgatttc aaattagttt
      901 a
//
LOCUS       DAYYHS010000226          893 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000226 DAYYHS010000000
VERSION     DAYYHS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.233"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..650
                     /locus_tag="ACHA6A_003068"
     CDS             <1..650
                     /locus_tag="ACHA6A_003068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971169.1"
                     /translation="IKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLG
                     TINNIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIY
                     DNAEKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYNV
                     GINYNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
     gene            640..>893
                     /locus_tag="ACHA6A_003069"
     CDS             640..>893
                     /locus_tag="ACHA6A_003069"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8971170.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYIL"
BASE COUNT          373 a           94 c          151 g          275 t
ORIGIN      
        1 tcataaaaat ttccggagaa tcaaaaaata tgaccattta tccatgttct ttcaatgtag
       61 atgaccaaaa tgattatgga gagaaaatag tcaataaaag cttagagtct acgttgaacc
      121 ttgcttattt aggtacgatt aataatatcg tagatttaga agcgattaat gaattattga
      181 aaacatgcaa aagaaaattt ggaaacaaaa taaatcttta ctttattgga aaaggtgaaa
      241 gcaaaactaa attagctgat atttgtgaaa aaaatcatat tgaatttgtt gactgcggtg
      301 ttatatatga caatgcagaa aaaaaagtta tactcgataa atgtcatttt ggactaaatt
      361 tatttaagtc aaataccata attggtttaa gtctgaaatc gattgagtat tttaggtttg
      421 gattacctat aatttcaaat ttatcaggag atacgaaaga aatagttaaa agatataatg
      481 tcggaatcaa ttataatagt accgttgatg aaaatgagat gttgaaattt tatcagtgta
      541 ataaaaacat aaatgaaaat gttagaaaaa tttacgccac tatattctct gagcaagtat
      601 tgaaaatgaa atggaatgaa gtgtacaagg tgatgaacga tgaaaaataa aattgggata
      661 attattgtta cctacaatcc agataataac ataaagttat tagtggatac gttaatacaa
      721 gagaaacaga acaaacttat tatcgtggat aacggctcag ataatattaa ctcttttcaa
      781 tttgaaatga tggaaaactt aattttaaaa aaaatgaatg agaacattgg gcttgcgaca
      841 gctcaaaaca taggtattga aattgccaaa aaagagaaat gtgattatat cct
//
LOCUS       DAYYHS010000227          846 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000227 DAYYHS010000000
VERSION     DAYYHS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.235"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..643
                     /locus_tag="ACHA6A_003070"
     CDS             68..643
                     /locus_tag="ACHA6A_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8971171.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(712..>846)
                     /locus_tag="ACHA6A_003071"
     CDS             complement(712..>846)
                     /locus_tag="ACHA6A_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8971172.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          236 a          143 c          181 g          286 t
ORIGIN      
        1 gttcttttaa atgcacaaga agaaacgatt atgaaagatt tggaaaggtt aaagatgggc
       61 taacaaaatg aataaaaaca aaatggcaac tcgcgtactt acagtgatgg ctttgtgtat
      121 tgggatcaat tatattggag gaacgattgc tttatggctt cgtttgccaa tctatctcga
      181 ttctatcgga acgatttttg ctggtgcatt gcttgggcct gtacctggaa tgctgacagg
      241 attgtccagc ggtattttaa gtggagtgac tacagatatt ttttctctgt attattcgcc
      301 aatccaaatt ctcacaggct tgcttgctgg attgattttg ccaagatatc tcggcaaaga
      361 taaaaacagc ttgaaactgt tattactcgc tttgattttg tcggtacctg gaacgatcgt
      421 ttcttcgatc attaccgttc agttatttgg cgggatcacc tcctcaggat ccagtatgat
      481 cgttcagcta ttacatgggt taggtttaag tcagacaaca agtgtgatct tggtacaggc
      541 gggtacagac taccttgatc gcttgttgtc tatcgtcgtt gtttcattag tcatcactat
      601 gttgccccaa cgcttgatat attcaaaaag aaaaataggc tgaataaaca gagctggatt
      661 agcaaaataa tgctaatctg gctctgtttt ttcattttat tatttgtcaa attaagcgag
      721 acaaaacttt ccatttacgt aactcaaaaa aacttctaat ggtgtttggt aatttagtga
      781 ttttctaggg atatggtttc ttttggacgc aacggatgag atgaatcctt gattgacttg
      841 gttgaa
//
LOCUS       DAYYHS010000228          829 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000228 DAYYHS010000000
VERSION     DAYYHS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.236"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..249
                     /locus_tag="ACHA6A_003072"
     CDS             55..249
                     /locus_tag="ACHA6A_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971173.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            518..817
                     /locus_tag="ACHA6A_003073"
     CDS             518..817
                     /locus_tag="ACHA6A_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971174.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
BASE COUNT          347 a          101 c          143 g          238 t
ORIGIN      
        1 tcacttacat atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat
       61 tatgtagaag taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac
      121 gaatatcctt tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata
      181 aaaataaaga acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc
      241 gaaaagtaaa tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc
      301 taagattata gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt
      361 tgtataccat atgattattt caatatgtat cctggaattg gagatctcgt agagctatat
      421 caagatgaat caaatatact tgtcattcat catgaaatat agagacttat aaaattgttt
      481 atttttataa tgtatcagta tagaaaggaa ataaaacatg gattatactg ttgaatttga
      541 cagtgaacta cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc
      601 ccacaaagtg acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg
      661 ttcggcaaaa ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc
      721 atttgcttta aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat
      781 tgtatttgat aaagaggtga gtaaaaaaat aaaatagctg agcattgga
//
LOCUS       DAYYHS010000229          822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000229 DAYYHS010000000
VERSION     DAYYHS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.237"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..242
                     /locus_tag="ACHA6A_003074"
     CDS             <1..242
                     /locus_tag="ACHA6A_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8971175.1"
                     /translation="NASIGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNI
                     IDVSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(333..698)
                     /locus_tag="ACHA6A_003075"
     CDS             complement(333..698)
                     /locus_tag="ACHA6A_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971176.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          307 a          113 c          138 g          264 t
ORIGIN      
        1 gaaatgcaag tataggtttt agaactaaac gaagtctgtc ttctaaaaaa aattgggtat
       61 actcacaaac tattttttat ggaggaattg ttttaattag tttactttct tcaacacttt
      121 attctttgaa tattatagat gtttccacct caaattcaat atctattatt ggaatcatta
      181 ttgctgcaat tattacacaa ctgtttttag tgtttggaga gaagaaaaga agtaaaaaat
      241 agaaaaacta gtgatagttt tggtgatata agaaaaacat atatgtataa aaagaagaaa
      301 gtagcttctc tttgtacata tatggtttgt ttttatggat gccgagcttt tttataagat
      361 aaggctaccg cccataacga cccaaggatt tgtggaacta agtaaatacc tgcaagaagc
      421 aagatcagct gggtcggttt taaaggatga tagaagtaac gccattcagt aaaattggcg
      481 caaaataaat aggaagtgac agtagatata atattggcaa tgaataaaga tataatacgc
      541 ctgttccttt gaaaataata tccagcaaaa agagataaaa atatcaatgt gatgaacccg
      601 cttacggaat gataacgaaa atcaagcagt aaggcgaaat gcacataagg aacaaaagat
      661 aaaagcaacg gaaggttttt ccagcaagtt tttatcatct ttcatcacca cctaacaagt
      721 gttattattt tgttagtttt attgtaaatt gaatcctgca ataatcaatt actttttcat
      781 tatttctaga ttaaatgaat aaactctcat gccattatct ct
//
LOCUS       DAYYHS010000230          811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000230 DAYYHS010000000
VERSION     DAYYHS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.238"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACHA6A_003076"
     CDS             <1..672
                     /locus_tag="ACHA6A_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8971177.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAYYHS010000231          723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000231 DAYYHS010000000
VERSION     DAYYHS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.239"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..685
                     /locus_tag="ACHA6A_003077"
     CDS             89..685
                     /locus_tag="ACHA6A_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8971178.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          200 a          115 c          139 g          269 t
ORIGIN      
        1 tgttttctgt catatcaaaa aatggcgcag ttagttgctt ttctagtaaa gtatagttaa
       61 caaagaacct ctaaggagtt gatgaataat gaatattgct gcctctcgaa aactgataaa
      121 tttcatatcc attatcggat taggcctatc aatcgcgctg acgatttatt ttattaattt
      181 aggtgttttc aaagatttga atgcgttaag aggattagta ggtgattcca tcattttagg
      241 accgattatt tttgtcttca tccaaatcct gcaagtagtt attccaatca ttcctggagg
      301 gatcagtact gcagctggtg tcctgatatt tggcccttat gctggattta tctataacta
      361 tattggaatc tgcattggtt cgattatcat ttttctgcta gggcgtcgtt atggaaagcc
      421 attcatactt tccatgatta gcgataagac atataacaaa tatattggct ggctggataa
      481 tcaaaaccgt tttgagaagc tgtttgctct agctattttt cttccagttg ctcctgacga
      541 tgcgttatgt ttgatggctg gattgacaaa catgtcagtg aaacgatata cactaatcat
      601 tttgattgct aaaccactgt cgattttctt atatagtatg gccttgattt atggaggtca
      661 atatctaagt ggtcttcttg gataatattt attttgtaaa attagtcatt gattgacaat
      721 gtt
//
LOCUS       DAYYHS010000232          712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000232 DAYYHS010000000
VERSION     DAYYHS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.240"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..358)
                     /locus_tag="ACHA6A_003078"
                     /pseudo
     CDS             complement(245..358)
                     /locus_tag="ACHA6A_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          261 a          106 c          121 g          224 t
ORIGIN      
        1 aaagagtata cgaacaaacg ttctttttgt aaaggtggaa tttattatac taaataaata
       61 ggtgaacaag tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa taaaacacaa
      121 acaaaaagaa atcctattat gataacattt cttataataa caaacacaac agaagacgtg
      181 taatagttag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt
      241 ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa
      301 gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa
      361 acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg
      421 tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca
      481 gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg
      541 taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg
      601 ttaatactta caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc
      661 gaaataaagg atctaagcca atagcttaga tcctttattc tatgctttat at
//
LOCUS       DAYYHS010000233          686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000233 DAYYHS010000000
VERSION     DAYYHS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.241"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACHA6A_003079"
     CDS             complement(<1..448)
                     /locus_tag="ACHA6A_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM8971179.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          205 a          137 c          105 g          239 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tctatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaa
//
LOCUS       DAYYHS010000234          654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000234 DAYYHS010000000
VERSION     DAYYHS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.242"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..652
                     /locus_tag="ACHA6A_003080"
     CDS             <1..652
                     /locus_tag="ACHA6A_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8971180.1"
                     /translation="LALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIA
                     KNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLI
                     EEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIE
                     NFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          281 a           83 c          107 g          183 t
ORIGIN      
        1 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
       61 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      121 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      181 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      241 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
      301 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
      361 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
      421 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
      481 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
      541 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
      601 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAYYHS010000235          618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000235 DAYYHS010000000
VERSION     DAYYHS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.244"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACHA6A_003081"
     CDS             <1..231
                     /locus_tag="ACHA6A_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8971181.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            390..>618
                     /locus_tag="ACHA6A_003082"
     CDS             390..>618
                     /locus_tag="ACHA6A_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8971182.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
BASE COUNT          213 a          112 c          102 g          191 t
ORIGIN      
        1 ttaaaaaatc ttcctgaaac acttgatgca caacttcgca cgaaacttca aaacctttta
       61 acgtatgaag aaggtattta caatgctatg atttacccgt attctaatgg caaaatagag
      121 gccaagattc cccatatcaa gacactgaaa cgattatctt atggatttaa atcatttgag
      181 aacatgaaaa tcaggatttt tttgatcaat caattgattc aagtgaaata acaaaaaaat
      241 ctgagacaga aggtcagtcc ttctatcggc tctatacttt ttgatgttgg tgaacttcac
      301 caacatcttt tttgtataaa aaaatataga aaagacaccc tgaaatcctt tagaattaat
      361 ttggctacca attcatagga ggattcaaga tgtctcatac tcagcttatc aaagataccc
      421 taaatattct agacctaaat atctatttcg aggaaaattg tttaactaaa gaaaaataca
      481 aagggcaaat ttgcttgatt tacaaaggaa ctcttctgta caagccagaa gagtgttccc
      541 attgtctttg tgtcgtcccc tctaggatca ttcgttgggg gacgacaacc gttcggttgt
      601 tactgaatga tgtgtctg
//
LOCUS       DAYYHS010000236          613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000236 DAYYHS010000000
VERSION     DAYYHS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.245"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>613
                     /locus_tag="ACHA6A_003083"
     rRNA            <1..>613
                     /locus_tag="ACHA6A_003083"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          151 a          137 c          192 g          133 t
ORIGIN      
        1 ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt ggaattccat
       61 gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg
      121 tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta
      181 gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag
      241 ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt
      301 gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct
      361 taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc gggggcaaag
      421 tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca
      481 acgagcgcaa cccttattgt tagttgccat cattcagttg ggcactctag caagactgcc
      541 ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt atgacctggg
      601 ctacacacgt gct
//
LOCUS       DAYYHS010000237          603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000237 DAYYHS010000000
VERSION     DAYYHS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.246"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACHA6A_003084"
     CDS             complement(<1..450)
                     /locus_tag="ACHA6A_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8971183.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
BASE COUNT          177 a          105 c           73 g          248 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aat
//
LOCUS       DAYYHS010000238          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000238 DAYYHS010000000
VERSION     DAYYHS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.249"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..>588
                     /locus_tag="ACHA6A_003085"
     CDS             33..>588
                     /locus_tag="ACHA6A_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8971184.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFK"
BASE COUNT          243 a           95 c           88 g          162 t
ORIGIN      
        1 tactatcttt aaaaataaaa aggagaaaaa agatgaagcc aaatccttca gaagcaggaa
       61 aacgaatcaa acaattacgc ctttcatgtg gttttactat ggaagaactt ggaaggaaaa
      121 tagataattc accacgtgcg accatatcca actgggaacg tgggaccaat ttaccaaatc
      181 cacaaaagct gaaattatta tcaactatca ctaattcaac aattgactgg attaaatggg
      241 gaactttaga agaatatata acttcatacc ttatcgatat agggtatgag ctatatatta
      301 aggacttccc agaaatacct cacaaagtct ttaaagacat tcaagaaaga tatagtgata
      361 cgttttcgct agataaagac tatgagttat tgaatcctat aataaaaaat atatttacaa
      421 aatattactc aaaagatttt gaaatttatc taaatgagat acttgctaat aatatcaatc
      481 ctctgattga agagtacgct aaccaaagac actctatctc taaagagcta tttttcaaaa
      541 gagccaacag taaacttcaa gacaagataa gaaatggtga tttcaaat
//
LOCUS       DAYYHS010000239          564 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000239 DAYYHS010000000
VERSION     DAYYHS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.250"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..>564)
                     /locus_tag="ACHA6A_003086"
     CDS             complement(19..>564)
                     /locus_tag="ACHA6A_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8971185.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          162 a          104 c          100 g          198 t
ORIGIN      
        1 tgctctaaaa tgatattttt acacaactgt tacggttgtt ttacgaaatt ggacgggact
       61 ttgccagttt aatttctgtt ttatccgttc attattgtag taatagatat agctatcgat
      121 ggctgatttt aattcctcga aacagttata aactttcccg tgaaaaatct cttgttttaa
      181 taagccaaaa aaattctcca taagtgaatt atctaagcag ttccctttcc gagacatgct
      241 ttgaaagatt ttgtactcct tcaatttgtt tctataagct ttcatttgat acgcccaacc
      301 ttgatctgag tgaaacgtac gacgataggg acaatcgtta gtggtctgga tagcttcctc
      361 caaaccttcc attaccgcta atgcattggg cttttcagag atacgatagg ataaaatctc
      421 actattgaac atatccataa aaggatctag atacaatttc ttttgacgga taatgccttt
      481 ggcatccact tcatggtatt taaattcagt tgtatccgtc gtgattttct gatggcagat
      541 actggtgtag aaacgacgat ggat
//
LOCUS       DAYYHS010000240          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000240 DAYYHS010000000
VERSION     DAYYHS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.252"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHA6A_003087"
     CDS             <1..>534
                     /locus_tag="ACHA6A_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8971186.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAYYHS010000241          499 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000241 DAYYHS010000000
VERSION     DAYYHS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.254"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>499)
                     /locus_tag="ACHA6A_003088"
     CDS             complement(<1..>499)
                     /locus_tag="ACHA6A_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8971187.1"
                     /translation="KLDDSPSYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYK
                     LTQYFL"
BASE COUNT          144 a          101 c          105 g          149 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaattt
//
LOCUS       DAYYHS010000242          487 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000242 DAYYHS010000000
VERSION     DAYYHS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.255"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..208
                     /locus_tag="ACHA6A_003089"
     CDS             <1..208
                     /locus_tag="ACHA6A_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8971188.1"
                     /translation="FTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFEN
                     VVTGKENWNDLYHANEVLKIAEEK"
     gene            209..>487
                     /locus_tag="ACHA6A_003090"
     CDS             209..>487
                     /locus_tag="ACHA6A_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8971189.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRD"
BASE COUNT          188 a           54 c           98 g          147 t
ORIGIN      
        1 gtttacttta gatgaatgta tcaatgttga cttattaaat aataaaataa gttttgaaga
       61 aaacattgtt aaagaaatgc ctgaatctgc taatgatatg tatgagaagg agttgaaata
      121 tttctttgaa aacgtagtaa caggcaaaga aaactggaat gatttatatc atgcaaacga
      181 agtattgaaa atagctgagg agaaataaat ggaaatttta tttactattt gtggtagagc
      241 tgggtctaag gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc ccctagtcca
      301 tttcgcttta gcaatgatag atatctatca acaaaaacat cctgaacata attgcacgac
      361 ttgtttgaat acggacagta atgagttagt agaattgacg actaagttaa atccgtctgt
      421 gaaaattgta gaacgtaaac ctgaattagc tacagatact gtagggaaaa ttgaagtgat
      481 acgtgat
//
LOCUS       DAYYHS010000243          484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000243 DAYYHS010000000
VERSION     DAYYHS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.256"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>484
                     /locus_tag="ACHA6A_003091"
     CDS             <1..>484
                     /locus_tag="ACHA6A_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8971190.1"
                     /translation="VTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAK
                     WPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKED
                     LPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVE
                     G"
BASE COUNT          196 a           74 c          113 g          101 t
ORIGIN      
        1 aagtaacctt caccgcagga gaaggaacaa gtattgaaag tggaacaaca gtctttgcag
       61 tgaaagacgg cgtaagctta ccagaagata aacttccagt gttgaaagca aaagatggtt
      121 atacagatgc gaaatggcca gaggaagcaa ctcaaccaat tacagcagat gatacagaat
      181 ttgtatcaag tgcaacaaaa ctggatgata aatctgacgc tgacaaatat aatcctgaag
      241 gtcaaaaagt gactacagaa ttgaataagg aaccagacgc atttgaggga attaagaaca
      301 aagaagattt accaaaagat actaagtata cttggaaaga aaaagtagat gttagcgcag
      361 ctggaaataa aaaagggact gttgtagtga catatccaga tggatcatct gatgaagttg
      421 aagtagatgt tacagtgaca gacaatcgtt ctgacgccga taaatatgag ccaacagtag
      481 aagg
//
LOCUS       DAYYHS010000244          481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000244 DAYYHS010000000
VERSION     DAYYHS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.257"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..449)
                     /locus_tag="ACHA6A_003092"
     CDS             complement(<1..449)
                     /locus_tag="ACHA6A_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8971191.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYLK"
BASE COUNT          136 a           94 c           61 g          190 t
ORIGIN      
        1 ttttttaaat atgcgttctc tgctcttaaa cgaataactt cttcttcaag agactcatct
       61 ttaagttttt taggaatgtt tagcttggaa ttcatactaa tttttctgcc ccttttttgg
      121 ctctcaagtg aagaagcacc gccttcttca tattgctcta tccatttact tagagttcta
      181 tttgaaccga tattaaattt tttagcagtt tcttggatag aaagaccatt tgtttccata
      241 tattctataa catcaagttt aaattttgta gtgtagcttt tgccacctcc aaccaagcct
      301 tcccaaccat gatagttata aatccttacc caatgtctaa ccagtgtacg atttatttga
      361 tatttatgtg caagatattt gtagccgcct tcgttattta aatagtctga aactactttt
      421 ttcttaaaaa caaatgtata tttccgcaaa aaaagcaccc cttttaatta gatttctagt
      481 c
//
LOCUS       DAYYHS010000245          479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000245 DAYYHS010000000
VERSION     DAYYHS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.258"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..446
                     /locus_tag="ACHA6A_003093"
     CDS             <1..446
                     /locus_tag="ACHA6A_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8971192.1"
                     /translation="RPPIKIDLPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRK
                     ALEFLIKDYAISKNSEDEDKIKKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYV
                     RRHTNKDVHDMDAFIKSAAQFIAADYDADLADQFINGNNFMPTTN"
BASE COUNT          191 a           68 c           76 g          144 t
ORIGIN      
        1 accgtccgcc gattaaaata gatttaccag aaaatattga aaaagtttct cctacatttg
       61 ttgatattta tactcaagcc acacaagcta aatctgagca attggaacaa attgctggag
      121 ttggttatag aaaagctcta gaatttctaa ttaaagatta tgcaatatct aaaaattcag
      181 aggatgaaga taaaataaaa aaaatgttat taggtaaaac aattgaaaat tatttgaatg
      241 attttcctaa actccaaaga ttagccaaag ctgctacttg gattggtaat gatgaaacgc
      301 actatgttcg tagacatact aataaagatg ttcatgatat ggatgcattt attaaatcag
      361 cagcgcaatt tatcgcggct gattatgatg ctgatttagc tgatcaattt ataaatggaa
      421 ataactttat gccaactact aattaaaaga ataaaagagc cacagatacc aattaataa
//
LOCUS       DAYYHS010000246          475 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000246 DAYYHS010000000
VERSION     DAYYHS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.259"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..318
                     /locus_tag="ACHA6A_003094"
     CDS             <1..318
                     /locus_tag="ACHA6A_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8971193.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
BASE COUNT          185 a           69 c           64 g          157 t
ORIGIN      
        1 gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt
       61 aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga
      121 actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa
      181 agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac
      241 aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca
      301 ttagaatcaa tgttttaact tgatcaacag aatgggcttt tccatttaca caaaattatt
      361 gacgctcccg acaaaattat gatcttgcgt aaagttataa aatattaata aaaatatttt
      421 attaatgaaa agcttggtag cacggttatt catatgctaa tttatttcac tttat
//
LOCUS       DAYYHS010000247          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000247 DAYYHS010000000
VERSION     DAYYHS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.260"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACHA6A_003095"
     CDS             <1..>468
                     /locus_tag="ACHA6A_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8971194.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAYYHS010000248          437 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000248 DAYYHS010000000
VERSION     DAYYHS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.261"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>437
                     /locus_tag="ACHA6A_003096"
     CDS             <1..>437
                     /locus_tag="ACHA6A_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8971195.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGK
                     KWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV"
BASE COUNT          136 a           82 c          105 g          114 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 acctttacac aagaacagcc attggaagcc atcgttacgg atcttactta tgttcgcgtg
      301 gggaaaaagt ggcattatat ctgcttaata cttgatttgt ttaatcgaga aattattggt
      361 tattcctgtg gtgagaagaa agatgcctca ttggtaaaag aagcctttgg acggataccg
      421 tattctttaa cagacgt
//
LOCUS       DAYYHS010000249          425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000249 DAYYHS010000000
VERSION     DAYYHS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.262"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHA6A_003097"
     CDS             complement(<1..132)
                     /locus_tag="ACHA6A_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8971196.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          146 a           82 c           83 g          114 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaat
//
LOCUS       DAYYHS010000250          422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000250 DAYYHS010000000
VERSION     DAYYHS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.263"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>422)
                     /locus_tag="ACHA6A_003098"
     CDS             complement(<1..>422)
                     /locus_tag="ACHA6A_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8971197.1"
                     /translation="NNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSK
                     ECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKN
                     IEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEK"
BASE COUNT          134 a           61 c           60 g          167 t
ORIGIN      
        1 atttttcact ttcttttatt tgatctatat ccctttcatc aaaccatttg ttcatggaag
       61 gatcaataat atgaatattt gctaatttcg aagtttgtaa agtagtttct tcaatatttt
      121 tcaattcaag tggtaaatct ggtcctccaa taacattttt atttttgaac gaggaattaa
      181 aaaaaagtgt atgaaaaaat ttttcatctg gggcaaacat acctatttta aaattcaggt
      241 agtcttgttg attttgttca tacatttgta ttaattctgt taggcattct ttcgacaaag
      301 cccaccattg agaaccatga tacaaatcaa atttatggtg tctaattttt gggggaagtt
      361 taatcatcct cagacaacga ttaattatag ctctaaaaat tttaaatgca gttgtgttat
      421 tc
//
LOCUS       DAYYHS010000251          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000251 DAYYHS010000000
VERSION     DAYYHS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.264"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACHA6A_003099"
     CDS             complement(<1..339)
                     /locus_tag="ACHA6A_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8971198.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAYYHS010000252          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000252 DAYYHS010000000
VERSION     DAYYHS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.265"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACHA6A_003100"
     CDS             complement(109..>414)
                     /locus_tag="ACHA6A_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8971199.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAYYHS010000253          405 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000253 DAYYHS010000000
VERSION     DAYYHS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.266"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..243
                     /locus_tag="ACHA6A_003101"
     CDS             46..243
                     /locus_tag="ACHA6A_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8971200.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            245..>405
                     /locus_tag="ACHA6A_003102"
     CDS             245..>405
                     /locus_tag="ACHA6A_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8971201.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINR"
BASE COUNT          159 a           42 c           75 g          129 t
ORIGIN      
        1 gttagaataa tagtctaaaa acattaattt aggggtgatt agattatgaa acatttaaaa
       61 attttgtcta ttaaagagac acaacttatc tatgggggta ccactcatag tggaaaatat
      121 tatggaaatg gagtgtattg cactaaaaat aaatgtacgg tcgattgggc caaggcaact
      181 acttgtattg caggaatgtc tataggtggt tttttaggtg gagcaattcc agggaagtgc
      241 taaaatgaaa aaaaatgcta agcaaattgt tcatgaatta tataatgata tatctataag
      301 taaagatcct aaatattctg atattcttga ggttttacaa aaggtatatt taaaattaga
      361 aaaacaaaaa tatgaattag atcccagtcc tttaataaat agatt
//
LOCUS       DAYYHS010000254          345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000254 DAYYHS010000000
VERSION     DAYYHS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.267"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           65 c           56 g          111 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttct
//
LOCUS       DAYYHS010000255          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000255 DAYYHS010000000
VERSION     DAYYHS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.269"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHA6A_003103"
     CDS             complement(<1..>321)
                     /locus_tag="ACHA6A_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8971202.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYHS010000256          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000256 DAYYHS010000000
VERSION     DAYYHS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.270"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHA6A_003104"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHA6A_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYHS010000257          299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000257 DAYYHS010000000
VERSION     DAYYHS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.271"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>299)
                     /locus_tag="ACHA6A_003105"
     CDS             complement(<1..>299)
                     /locus_tag="ACHA6A_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971203.1"
                     /translation="TVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYW
                     VSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSL"
BASE COUNT          110 a           51 c           39 g           99 t
ORIGIN      
        1 caaagagaaa attaattctg ctaagcttat tattgaaaat actgataact tataattata
       61 tcgtccattg acaatcaaat ataaactaac tatacttgtt agaacaaaaa aacaggcgtt
      121 gacttgttct tgattagtta aaaatatcaa taaaggaata cttacccaat atatttttct
      181 atttatttct tctcctctta agagttgaaa aaacggataa aaagttagta aactaaatgc
      241 tactggccac tgatagttca aggtggttgc tctccaaccg gtttccccca ttactgtca
//
LOCUS       DAYYHS010000258          298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000258 DAYYHS010000000
VERSION     DAYYHS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.272"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          121 a           31 c           51 g           95 t
ORIGIN      
        1 tatattatta tttgttctaa atgattcctt taaggtgtca ctaaaaatta taaaaaatag
       61 tgttgacgca cttttgatgt catgttataa taacgaagtc gtttggatga gatagctaat
      121 aaagcttgat tttatcgaaa aaaacttcga aaaagttatt gacaaataac aataaagatg
      181 gtatgataat caagtcgctg ctaatgagtg gcaaagacgt ttagaaaaaa taaacaaaga
      241 agttgttgac attctcttct gaatctgata agattaaaga gttgttaaaa caaaaaga
//
LOCUS       DAYYHS010000259          291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000259 DAYYHS010000000
VERSION     DAYYHS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.273"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          106 a           36 c           39 g          110 t
ORIGIN      
        1 aaagtctgca tattcatcac tgatattttt tctaataaat tcttccatca taaaaatcac
       61 ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc gatatttgag gggcaaaaaa
      121 ggggcataat ttgactgata ttaagttagc tattgatata tgaatgacat acaaaaatat
      181 aaaatctttt aaaaatgctt atatatcaat gtttttgcca ttcttttata tatctgttat
      241 atgtcaaaaa aatgtgttca atttcaattt gtatttgaat aaagaggttg t
//
LOCUS       DAYYHS010000260          283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000260 DAYYHS010000000
VERSION     DAYYHS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.274"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>283
                     /locus_tag="ACHA6A_003106"
     CDS             <1..>283
                     /locus_tag="ACHA6A_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8971204.1"
                     /translation="ATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVW
                     NELLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT           89 a           57 c           54 g           83 t
ORIGIN      
        1 atgctactta tattccatta aaacggcaaa ccgtctccaa agaagcaatc tatattgcca
       61 ttggtatacg agaagacggc actaaagaag tactaagtta tgcgattgct ccgactgaat
      121 cgacctacgt ttggaatgag ttgttacagg acatttactc cagaggggtt catgatgtct
      181 tactgtttat taccgatggc ttaaaaggca tgaaagatac gattcaccag atttatccta
      241 aagctaaata ccaacattgc tgtgttcacg tttcccgtaa tat
//
LOCUS       DAYYHS010000261          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000261 DAYYHS010000000
VERSION     DAYYHS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.275"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHA6A_003107"
     CDS             <1..>282
                     /locus_tag="ACHA6A_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8971205.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYHS010000262          260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000262 DAYYHS010000000
VERSION     DAYYHS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.277"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           97 a           32 c           38 g           93 t
ORIGIN      
        1 accgaaaaaa ttatgtttag ataataccat agttaagttt ttccttgtga tgtttttcgt
       61 aaaaagatat tttagcagca ttatcgttac agtgcatagt taccatcggc gttatgacta
      121 tataaagtat actttcgttg atccagaaag gtatatagat taaaataagt tacctaaaaa
      181 ggacaaatac aaattttatt atcttattca cagtaagaag ataattttaa gattgcgaaa
      241 tacgatacta tttgtaatct
//
LOCUS       DAYYHS010000263          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000263 DAYYHS010000000
VERSION     DAYYHS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.denovo.278"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACHA6A_003108"
     CDS             <1..>253
                     /locus_tag="ACHA6A_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8971206.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAYYHS010000264         9784 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.guided.097, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000264 DAYYHS010000000
VERSION     DAYYHS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.guided.097"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..816)
                     /locus_tag="ACHA6A_003109"
     CDS             complement(<1..816)
                     /locus_tag="ACHA6A_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8971207.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQ"
     gene            944..1513
                     /locus_tag="ACHA6A_003110"
     CDS             944..1513
                     /locus_tag="ACHA6A_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8971208.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1580..2317
                     /locus_tag="ACHA6A_003111"
     CDS             1580..2317
                     /locus_tag="ACHA6A_003111"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8971209.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2548..3816
                     /locus_tag="ACHA6A_003112"
     CDS             2548..3816
                     /locus_tag="ACHA6A_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8971210.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4303..4767
                     /locus_tag="ACHA6A_003113"
     CDS             4303..4767
                     /locus_tag="ACHA6A_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8971211.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4751..5380
                     /locus_tag="ACHA6A_003114"
     CDS             4751..5380
                     /locus_tag="ACHA6A_003114"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8971212.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5734..7212
                     /gene="msr(C)"
                     /locus_tag="ACHA6A_003115"
     CDS             5734..7212
                     /gene="msr(C)"
                     /locus_tag="ACHA6A_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8971213.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7259..7705
                     /locus_tag="ACHA6A_003116"
     CDS             7259..7705
                     /locus_tag="ACHA6A_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8971214.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7904..8419)
                     /locus_tag="ACHA6A_003117"
     CDS             complement(7904..8419)
                     /locus_tag="ACHA6A_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8971215.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8536..9738
                     /locus_tag="ACHA6A_003118"
     CDS             8536..9738
                     /locus_tag="ACHA6A_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8971216.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3109 a         1725 c         2039 g         2911 t
ORIGIN      
        1 ctggtctttt ttccaaatga gattcagtga agcttgtaat ggcggatcga gtgggatgaa
       61 tactttttcc gtcccataag tattgatgat cccatctaga gctaatgcac caaaagtccc
      121 gttatcgacc atcaaagaag cattgtacaa taagttatat gttccgacga tcgataactg
      181 atctatgctg aaacctagcc attcagctag ttgactgtcc actgacgttt gccgggaaat
      241 gatcaaagga acagttttca gttcctgcgg atggatgact gtttgctttg ctaaaggatg
      301 attccgatgt atcaaaagtc cccaacgatc ttgggctggc agtcgaaatg actggtattt
      361 ttgtttgttt gtcggttcga ttacgagtcc aaaatcaagt aatccgctat ccagtttgtc
      421 tatgatatca tctgcattcc cgctatataa atgaaactgg atatcaggat gcgccttgat
      481 caacgaatga aggctatcag aaatgaaaga gattgcttta gtttcgcctc cgccaatgta
      541 gatttctcca gagatgattg cgtcatttgt taaattgttc gccgtttttt ctaccaacga
      601 caagatttct tttccttttg aataaagata tcgaccagaa tccgtcaact gtatctctcg
      661 ggagcctcga gtgaaaagtg tcacaccaag ttcttcttct aaatctttca gttgttttga
      721 gagagtaggt tgcgtgatat gcagagattt tgctgctcgg ctgatatttt tttctctagc
      781 tactgctaaa aaatacgtca gtatacgaat atccattttt tccctcctgt ttttaaaaaa
      841 gcataccact aagtatagtt aaaaggaata gttaagtatg ttttataagt attagacatc
      901 ataagccgtc cgttataaag taaggataaa aggagggagc aaaatgacaa gcctcatcga
      961 aaaacaaatc gtcaataaag agattcgagt aacggatccg ctctttgaag tgatccatca
     1021 gatccaagcg gaaaacgaag agcctttagc agcgctgaat actgggtacc atgaaccagc
     1081 agatatccgc aaacggctag aaaatatcat tagtgacaaa gtagatgata cggtgactgt
     1141 tctgctgcct ttttatacag atttcggcaa acatattagt atagggaaaa atgtctttat
     1201 caatcgtcaa gtgatgttcg ttgacttagg tggcatctgt ttagaagatt cagtattgat
     1261 tggtcctcgt gtcaacctca tcacggtgaa ccatctaacc gatccaaaag aacgaagagg
     1321 cttgtcagta aagccaatcc acatcaagaa aaatgcttgg atcggagcag gagcaactat
     1381 tctgccaggc gtaacgattg gcgagaatgc cattgttgca gcgaatgcaa ctgttacgaa
     1441 agatgtccca gacaatacca tcgttgcagg aatccctgcc aaaatcgtca aacctgtcga
     1501 acggattatt tgaacagtaa gatgaaataa gagatagtga gtataagcaa gagatttttc
     1561 aaaacaggag gagaatagaa tgaatgattt atcagaaaaa gtgattgtca tcatgggcgc
     1621 ttcaagcgga atcggtgaag caacaacgaa gctattagca gaaaaagggg ctaaactagt
     1681 tatagctgca cgccgagaag atcgcctaaa agcaataaaa gaatctttac cagaagctga
     1741 gctatatatt caaacagcag atgtaagaga ttttgcacaa gtccaagcag ttatcgattt
     1801 agcgatggaa aagttcggtc gaatcgatgt attgtacaat aatgcaggaa tcatgccaac
     1861 cgctccatta gttgaaggtc accgtgatga atggcaaaat atgcttgata tcaacatcat
     1921 gggtgtgtta aatggaattt ccgccgtact tccaatcatg gaaaaacaaa aatctgggca
     1981 tatcatttca actgattctg tggcgggaca tgttgtttat cctgattccg ccgtttattg
     2041 cggaacaaaa tttgctgtcc gtgcaatcat ggaaggatta cgtcaagagc aacggcaaaa
     2101 taacatcaaa tctacgatca tctctccagg agctgttcaa actgaattat atcaaaccat
     2161 cagcgacaaa aaggctgctc ttgaacttca tgaagcgcaa aaagaatggg gattaacatc
     2221 tgaagatata gcttctgctg tagcatttgc gatagaaacg ccagaccgga tgtctgtcag
     2281 caacatgatt atccgtccga cgacacagga agtataatcc ataacggata atatccagag
     2341 agaattactt gttaaatagt tattcaaacg accatcaatt taaggcgtga atggagaaga
     2401 atattcttca tttacgtctt tttatgcaga aaaagcaaat ttattaaaaa gaaacaaatg
     2461 ttatttaaat atggacattg acttgctttt acttttttag ttacactgtt atttaggaaa
     2521 agtttcataa ttcaaaggag aaaaaagatg aaagcaaaga atttattatt tagcgttatt
     2581 cttttaggaa atctagcagt tgccaacaca acagtgctag ctgaagaatc gacagaaact
     2641 gtcgcaactc agacatcgag ttcttcagaa acaggaattg aaaacgatac agaaagtact
     2701 ttgcagtcag ctactgtctc agaaaataca atttcaaccg aagaaccaac aactgaaaca
     2761 acagaacaaa cacaaaccga agacagtaca tcagcagaag ctcagaacaa tccagagaat
     2821 gcagaagaaa tcacgaacca attgacagat gggcaaacat tgacacatac ccatcaagca
     2881 gatttgtctg aattcgaaaa ctatactggc tatgcttttt atgctcgctc atctgctaat
     2941 tctcaacaag cgttcattga tagtattgcc tcaactgcac aaagtttggc aagtgccaat
     3001 gacttatatg catcagtcat gattgctcaa gccatcgttg aaagtggctg ggggaacagt
     3061 actttggctt ctgcccctaa ttacaatcta tttgggatta aaggaagcta taatggccaa
     3121 tctgttacga tgcctacttc ggaatatgtg aatgggcaat ggattacggt gaatgccgct
     3181 tttagaaaat atccttcgta caaagaatca ttgcaagata acgtaacggt attgaagacg
     3241 acttcttttc agccaggcgt ttactactat tccggtgcat ggaaaagcaa tacgaactct
     3301 tacaaagatg ctacggcttg gctaacaggt cgttatgcca ctgcgcctaa ttatggttct
     3361 acattgaaca atgtaatcga aacctataat ctgacacagt atgatacagc accaacttca
     3421 acttctccga tgtatcgtct gtataaccgt catacaggag aacatttata tacattgaat
     3481 gccggagaaa aaaattatct gccaactgta ggctgggaat atgaagggat tgcttggcaa
     3541 gcaccaaaca gcggacaacc tgtctaccgt ttatacaatc cttacagtgg agaccatcat
     3601 tacacaatgg cacaatctga gatcaactct ttaacaaaaa tcggttggcg ttatgaaggt
     3661 ctaagttttt attctggtgg ttccaagcca atttatcgac tatttaatcc aaatgaaaaa
     3721 actggtaccc atcactatac cttgagtgct aaggaaagag attggctaac accgatgggc
     3781 tggagatatg aaggaatagg attttatggt tattaaaaag aggtagtgaa aaagctgccc
     3841 tgcatcaagt agtaagaaca gccaaacaaa aacagatttt catgtttttt gtttggttga
     3901 acttattacc gcagatgcaa gtttttgaac accgtttact caaaaaaggt tgtgacaaca
     3961 tcttgtcata acttttttat tactgtttaa gcatactgat ccaatttcta atattcgacg
     4021 ggattcttgc aaaaggcgat ttcggaaatt tttggcggtg taaaattatc tactgcagaa
     4081 gagctatctt ctatagtttg ataagaatca ataatcttgg ttttagcgtt gtgctttctt
     4141 tatcattttt tccgttcatt ctacaaaaaa tttcttttct ctatactttt ctattgagag
     4201 cgtttttttt attacacagc tgattctttg acttttttct ttttttattc tattattgac
     4261 aagtcaatcg ttgacgagtc aattattaga aaggaggaaa ttatgtcttt agaagagatt
     4321 agtcaccttt tatatcaaat caaattagcc gatcaaaaaa tcgtccagat ctttgaagaa
     4381 aatatcggta taagtcttac tagatacgag atcatgatga gactgaaaga agaaaatcca
     4441 ttgctacaaa gcagtttgca agaaaagttg cagattgatc aaggagcagt tactcgtcat
     4501 ctgaaggtat tggaagcaaa tggctatgtt tcgcgtcaga gaaatcctca gaacaaccgg
     4561 gaagtgtatg tagcgctaac agaaaaagcc cagaagaaaa tcaagcattg tgaaatggac
     4621 cataaagata caggttccgt gctatccccg gctttcagcg atgtagaaat gaaacaattt
     4681 ctaacacttt tagataaatt gaatacccaa ctttgcaata taacaacaga aacgaaagag
     4741 gttaataata atggaacaaa aaaatgattt ttcaacaatt atgactggta gaagatccgt
     4801 tcgcgtatat gatgaaacat acaaaatccc acacgaagaa atgctggaaa tgatcaaaga
     4861 agcgacgact gcaccttctt ctgtcaatat gaaaccatgg cgctttgtgg tcgctgaaag
     4921 cgaggaagca aaagaaatct tgcgtccttt gatccgtttc aatacgagac aaaatgatac
     4981 ttcttctgca atggtcatga tttttggcga tatgcaatgt tacgaatatg gcgaagaaat
     5041 ctacaaccaa gcttacgaat cagggaagat gccaaaagaa gtcagagatc aacaattagc
     5101 tgcgatcatt ccttattaca agagtttttc aagagaagaa atgaacgatg tagtcaaagt
     5161 cgattctagc ttagcggcta tgcaattcat gttagttgcg cgtgatcatg gctatgaaac
     5221 aaatccaatt ggtggattcg aagcagatca gttggcagag gcatttggtt tggataaaga
     5281 ccgttatgtt ccagttatta ttttatcaat aggaaaagcg gtagaagaag gttacgagtc
     5341 tgttcgttta gcaccagaaa agatcactat gttcgaataa aaggttttca gttactattc
     5401 agtagctatt gctttttttg aatcgtgtgt ggtaacctaa taaaaaagga ggtcggaaaa
     5461 atgactgcat cgatgaaatt acgtttcgaa cttttgaata acaactaaat aaattcaaaa
     5521 gacatgtata aacaacaatt tcatggatgg agtatgtcca ttttttcgaa cgacagtaga
     5581 ataggtgaaa gctatgacag atttggtgat ttagtcatag ttatttcgtt cagagtagga
     5641 aaagatgtga tgcagttttt ttgcaaacag atttttctgc tcttttttag tatcgataat
     5701 tgttgtttta catgaaatag gagtgtaaaa catatggaaa atttagcagt aaatataaca
     5761 aatctacaag tcagctttgg caaccagcta gaactatcta ttgattctct tcgtgtctat
     5821 cagcaagatc gtatagggat cataggagaa aatggtgtcg gtaaatcgac tttgctcaaa
     5881 ctaatagccg gtgaactttt tcccgatcat gggaaaatcc aaacagagat caccttcaac
     5941 tacctgcctc aattaaccta tctcgctgaa gcaaaggacc taaatttgga attagccagt
     6001 catttccagt taagactgga agaaacttca gagcggaaat ggagcggagg ggaagaacga
     6061 aagatcgagt tgatacgtct tctttcttct tatgaacaag ggatgcttct agacgagcca
     6121 acaacccatc tagatagaaa aagtattgat cgactgattg aagagcttcg ttattattat
     6181 ggtacgctgg tttttgttag tcatgatcgc tattttctag atgagctagc atcgaaaatc
     6241 tgggaagtaa aagacggaga aatccgagag ttttcgggga attatagtgc ctatctcact
     6301 caaaaggaat tggagaaaaa gactcagcta cgagaagcag agtcgatcat gaaagagaaa
     6361 aaacgattgg aaaaatcgat ccaagaaaag aaaaaacaag cggaaaagtt ggaaaaagtg
     6421 tccagtaaaa agaaaaagca acaaatcaga ctggatcggt tgtcttcctc taaacaaaaa
     6481 gacagtgtac aaaaagccat ccaaaagaat gcgaaaacat tagagagaag actccaaaaa
     6541 ataggagaaa caaccaagcc gcagcagatg aaacaaatcc gttttccagt accaaaatct
     6601 cttgaactcc acagccgcta tcccatcatg ggacaaaatg tccaattgga aagaagtggg
     6661 agaacattac tggtaaatgg tgattttcag ttttctttag gtaaaaaaat cgcgattgtc
     6721 ggtgaaaatg gttcaggtaa gacaacctta ttagaacata tccgcaaaca aggagaagga
     6781 atccttctct ctccgaaagt aagctttcaa gtatatcagc aaaagggtta tcaaatgaca
     6841 tctgaagaat ccatcattcg ttttgtcatg agacaaacag agttttcaga atcgcttgtc
     6901 cgtagtttgc tgaatcactt agggtttgct caggaaactt tgacgaaacc gttatgtaca
     6961 ttaagtgggg gagaagcgac ccgtctgacg attgctttgc tttttactaa gccaagtaat
     7021 gtgttgctgt tagatgaacc gactaatttt attgatatgg caacgatcga agctttagag
     7081 aagctaatgc aaatatatcc gggaacgatt ttgtttactt ctcatgattc ttactttgtc
     7141 gagcgtacgg ctgatgaagt ttatgaaata aaagggcaga aaataaaaaa agtacttacg
     7201 agaaattttt aaatgtgcta cgatgatttt cgtgacaaat ttaaaggagt ggtacagatt
     7261 gcctacaaat aaaaaagaag gaataatttt tacaacaatg atgtgttttt tgatggtctt
     7321 ggggatgagc gcgtataatt tatggctgca tcaagatctt catttatcaa gtttgttgat
     7381 tggtctagtg cctggattcg tggtggcttt cattttggat gtcttcatca tcggtgtgct
     7441 ggcgaaaaaa attgccttta aactaccagt aaatccagaa agcaaggtcc aaatgatctt
     7501 aactatttca tgcttgatgg ttttaggaat ggtgacatgt atgtctttat tcggtgtgat
     7561 catggaacaa ggaatacctg atcaattatt ctctgcttat atgaatgctt ggaaaatgaa
     7621 tgtgattgcc gctttaccgt tgcagttgat catcgttggc ccgctttcaa gaagtgtgct
     7681 aaaagcaatt caaagtaatg aatgatggaa cactgttatt tgaaaaaagg ccttggcatg
     7741 ctttttgtca tagtctcttg aatgcagttc tagaaaaata gtgatccgaa cgataggttt
     7801 tggcctgtcg ttcggatcgc tttttctttt ccaactgtat tgctctttta ttgaagataa
     7861 tttgctcttc aatagatatt tctagaaagg ttctgtttat cccttactca aaaaactgct
     7921 gcatttgttc tcttacttgc tgacaccatt ggagttcctg ctgacaaagc ataattctgc
     7981 gctggatcac aacataacgt ccaaattctt gtttcttgtc tagcgagagg tcattcaatt
     8041 ttttttgatt tttttgcatg attttgttca gctgttgttc tctagtgaat agcagtccag
     8101 tagctgttgt tttatcgagc gtcgaaaaag catagatttt tgctagaaat tcatcttttt
     8161 gcgtaggagc gggagtttcc tctttgatcc attcctctac aagagcacgt ccttcatcag
     8221 tcaagtggta gatctttttt tgagagtgct cgtctttgtc caaaacggtc acataaccct
     8281 gctggtctaa cttggcaagc gttgtataga tctgactgtg atgggcttcc caaaaaattg
     8341 tgatcagctg cttcaattca tacccagaac gtggtttgga taaaagcacg cataataata
     8401 tataggctaa agtattcata aaaatcctac ctctcgtctt attttcttta ttatatcaca
     8461 gtaaagtttc cacgttttac atgtgcatat tgacatgtaa aataaatcat gtatgatgct
     8521 aatagagaga aagggatgga aatattggag aatcaaatag atatacaaga acaaacaaat
     8581 gaagaaacaa aggggaaaag ttcagcgcta gttgtagcag ctacctgtat gatcgctttt
     8641 atgggagtgg gtctagtaga cccaatctta aaaacgattg ctgcacagtt aaacgctacg
     8701 ccagctgaaa cgaccctgtt atttactagt tacatgttag tcactggtgt ggtgatgcta
     8761 tttacaggtt tcttatctag tcggatcggt ttgaaaaaaa cattgatgac aggattgttt
     8821 atcatcgttg tattcgctgg tcttggcgga ttatctggga atatcggaat gctgattggg
     8881 ttacgtgccg gctggggtgt cgggaatgct ttatttgtct ctactgcttt agcaacgatc
     8941 gtcagtattt tagttggtaa cacagaaaaa gcaatcatga tgtatgaagc agcacttggc
     9001 tgcggaatgg ctgttggccc tctagtcggt ggggtcttgg gcagtggttc atggcgttat
     9061 ccattttttg gggtagcatt tttgatgttt tgcggtttta tagccctagc ggtactttta
     9121 cctgaaactg agaaaccaaa gagaaaagta ggcctttggg aaggtgtcaa agcgttaagc
     9181 aatcataaac ttcgttcagt tggactaatc gcattgttat ataatttcgg attctttact
     9241 ttacttgcgt atgcgccatt tttattgatt ggctattctg aattagaagt cggttttgta
     9301 ttttttggct ggggtgtgct tctggctatt gcttcgattt tcgttgcacc ggctcttgag
     9361 cggaagttta cgacttatcg gacaatttta gcagcatttg tcttattcat tatttgtttg
     9421 ctgttattag gagctggaac ttctatgcca gtccttgtac cgatcagcat tgtgttagca
     9481 ggatttttcc aaggaattat caatacactg ttgacgacga ttgctatgga aataccaggt
     9541 ctgcaacgaa acgttgcttc gtccagctat agttttgtcc gctttttcgg tggagcttta
     9601 gcaccattta ttgcaggtgt gattggtgaa catattggtg aaagctatgc gttttatttt
     9661 gcaggtgcca ttgttcttat cagtgtatgt tgtatcattt atcaccgtaa ttattttgct
     9721 acaaatgaag aagaataaga aatcagaggt tgtgaaaaag ctgtcctgca tcaagtcata
     9781 agaa
//
LOCUS       DAYYHS010000265         9374 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.guided.102, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000265 DAYYHS010000000
VERSION     DAYYHS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.guided.102"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..245
                     /locus_tag="ACHA6A_003119"
     CDS             <1..245
                     /locus_tag="ACHA6A_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8971217.1"
                     /translation="KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILS
                     ELLEFYDAENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(395..1336)
                     /locus_tag="ACHA6A_003120"
     CDS             complement(395..1336)
                     /locus_tag="ACHA6A_003120"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8971218.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1462..2583
                     /locus_tag="ACHA6A_003121"
     CDS             1462..2583
                     /locus_tag="ACHA6A_003121"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8971219.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2841..4343
                     /gene="eat(A)"
                     /locus_tag="ACHA6A_003122"
     CDS             2841..4343
                     /gene="eat(A)"
                     /locus_tag="ACHA6A_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8971220.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4410..4937
                     /locus_tag="ACHA6A_003123"
     CDS             4410..4937
                     /locus_tag="ACHA6A_003123"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8971221.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5148..6209
                     /locus_tag="ACHA6A_003124"
     CDS             5148..6209
                     /locus_tag="ACHA6A_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8971222.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6212..7108
                     /gene="murQ"
                     /locus_tag="ACHA6A_003125"
     CDS             6212..7108
                     /gene="murQ"
                     /locus_tag="ACHA6A_003125"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8971223.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7122..9104
                     /locus_tag="ACHA6A_003126"
     CDS             7122..9104
                     /locus_tag="ACHA6A_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8971224.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9169..>9374)
                     /locus_tag="ACHA6A_003127"
     CDS             complement(9169..>9374)
                     /locus_tag="ACHA6A_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8971225.1"
                     /translation="EYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPFLA
                     DYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3065 a         1673 c         2047 g         2589 t
ORIGIN      
        1 agaaactagt gacggataat caagcgattg ctcattatca tttttatcgt cctgaaaata
       61 tttttttgct tcgagaacgt gatttagatg aattaccgga atttctgcat acaccattta
      121 agccaatcct atcagagcta ttggaatttt atgatgcaga aaattgggca aaacgattta
      181 tcactaacga ttccactatt tatgagcaat acgaatatga cctctcctct gttgaaaaca
      241 aataaaaaac ctacattttg aagagattat gtactgaaac aaacaagcac aattcttatg
      301 gcatcatgag agttgtgttt tctttttgat cttttattgg gcttaaaaaa agagaaccag
      361 ctgctgattc agctgattct catcagagag ccttttattc tgcgccaagg attccttctt
      421 ttgcatggac taattgggtc aaaaaggcac aatatggtgt agcaatatca tatttttgtc
      481 cttttcgcca aatggcacca ttgatgtagt cgatttcagt caaacgatgg tttttgatca
      541 ggtcttggta catagatggg taatgcaacc cgatgttgtc aggatcataa gtagaagcaa
      601 tatgttggta cacttcttct tgatctagta tgattccttc ttttgctgcg atagaagcaa
      661 attcagatac gattgttcga accatgctct ctgccgcttt ttgcgcgcct agttcagcaa
      721 tattacaatc caaaatcgtg cataaaccat tcaatgttcc atttacacaa gcttttcgcc
      781 aaatggaata acgaacgtta tggctataga tcggatttaa atttgcttct tgaaagactt
      841 ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa gttctctagt tcgatttctc
      901 catcacctaa caatgtgact tttccgggac cagctagacc agctgtccac atcgtgatcc
      961 caaataagat atttttcttt ggcacatatt tttccagtac atcttcatga cctaatccat
     1021 ttagtaaaca caaaacataa gtattgtcag taataatgga ttggatcgaa caaaacatat
     1081 catctaattg attggctttt gttaatgcta cgatcaagtc cacgtgttcg ttgctttcga
     1141 tgatttcttc tggtgaatag attggcagtt ttgcgaccac ttctttaccg ttaaagtcag
     1201 caatcaaccc atttgtacga atggcttcaa tgtgagcagg ccaacgatcg atcaagagga
     1261 cctcatttcc gctttgatgt agcatcaatc cgattcggct gcccattgct ccggccccag
     1321 ctattgcgat tttcatgttt tttctctcct ttattcaatg agttcccttt tagtatatag
     1381 aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat gaaaaaaagt gtcttactta
     1441 agaagaaagt gagggataca gatggatatt ttggcggcaa ttgattcatt taaaggaagt
     1501 gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg taaaggaaga
     1561 aaaatcaatg tgccgattgc agatggcgga gaaaatactg cagaagcaat ctattacgcg
     1621 ctcaggggaa catggcgtac cattgatggg atggatctct tattccgtca gcgaaaaatc
     1681 agctatttat tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc tgcacaagca
     1741 ttggggttag atctacttga taaaccaacg gatcaaacta tccgacttgc ttccagttat
     1801 gccttaggag aacttattaa agatgcactg cttcaagaag tagaacaaat tattgtcact
     1861 ttaggaggaa gcgggtgtag cgatggtggt ctaggattac tccaatcctt gggtgcttct
     1921 ttagaagggt acacagaagg aaatccttta ttatctacta aaaaacttca ttttgatcga
     1981 agaaacgttc ttgtttctaa agtaaaattg attatcgctg cagatgttac caatccatat
     2041 acaggtcaac aaggagctgc ttggcaattt ggaagacaaa aaggaggaat agatacgacg
     2101 ctagctttct tagataaaca agcaggaaag attgctcagc aaataaaaga tttctgggag
     2161 gtggatttag atgatattcc aggaagtgga gcagcaggcg gtattggtgg agcccttttt
     2221 ttgttgggag cgaagatggt ttctggtttc gatttagtca gccaaatcac tggattggaa
     2281 aaaaagatcg aacaagcaga tgtcatcatt acaggagaag ggcggatcga tcagcaaacg
     2341 atccacggaa aagttccata cggtgtagca gtgcttgcaa aaaaatatcg aaaaccaata
     2401 atcgctattt gcggaagtag agaccaagag ctagaaggac tttctgattt tctcccaatt
     2461 gtcttaagta ttcatttggg tccaataggt ttagaagaag caatggaaca caggcaaaca
     2521 ttagaaaaaa caaaaatctt aggacagaca cttagcagat ttttcaccat cctcccgaag
     2581 tagttgacaa aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg
     2641 atccgtgcat tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg
     2701 agacaaggaa gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct
     2761 ttttgtgtgt atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt
     2821 tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg
     2881 acagccaagg cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac
     2941 taggacttat cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca
     3001 aactacctta tcaagggcaa gtaatccatc agcaagaatt tgcctatttc ccgcaacaga
     3061 caaaagataa agaacgttta acctattacg tgttaaatga tattacggat tttgagatat
     3121 gggaaatcga aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat
     3181 tcagcacact atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg
     3241 acactcattt cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac
     3301 aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg
     3361 gatttatcga tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt
     3421 atcaagggaa tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg
     3481 ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag
     3541 ccgaatggtc tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata
     3601 ctgaatctag acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac
     3661 gatccaaagc aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa
     3721 aagatatcga atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac
     3781 ttttaagcgt agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt
     3841 ttacaatcga gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat
     3901 ccattatcca ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc
     3961 caaaacatct gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg
     4021 cggaatttgc agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc
     4081 ttgggatgga aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga
     4141 aaaaagtgga attggctaaa tctttatcac agccagctga actatataca tgggatgaac
     4201 cattgaatta tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga
     4261 aacctgccat gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga
     4321 ttatttctct tgagagaatc taacccttgt aatctcaaga acaacaaggt atggttaagg
     4381 ggaactttgc caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa
     4441 cttggaacgc acaaggatta gtttgcggac gggcagatgt gccgctgact gaaaaaggac
     4501 agatgcaggc acagaaacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa
     4561 tcatccattc ccctcttcaa cgagcaagag atacagcaca agcagttgct gatcgtctca
     4621 gcttgccgct gaccgttgat gaacgattgg tagaaatgga ttttggggat tatgatggac
     4681 tgccaagcaa agatgagaac tttcaaaaag cacgtttagc gttcgctgta cgattcccaa
     4741 atggggagtc agtcttagat gtgtatgcac ggatcgttcc tttattgaaa gaatgcatcg
     4801 aagacgaaga aaatgtctat ctgttagttt gccataatgc cttgataaga gtgatcaatg
     4861 cttattttca tccgatgcct aatgaggggt tctttacatt catggtagat aatacagaat
     4921 tggtttctta tgagtaatta tgtttttgtg ttatttaaat aaaatatttc aaggcactgc
     4981 ttttgcttag cagtgccttt tttaggttat gacaattctt ttgataatat aaactcaacc
     5041 tttagaaatg aaatttaatt attttaataa aagtattgaa attttgtttc tttagatata
     5101 tactctagtc atagaaatga agacggatac agaaaaagag ggataatatg tttggctttt
     5161 caatatttat gaacgattct ttgtcagaag agaagaaaca gtatatccgc caaatggcat
     5221 ccaatggctt tgtcggtatt ttcacttcca tgcatatacc agaagatgat gtttcggcat
     5281 atcaaaagcg attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta gaattgatgg
     5341 tcgatatttc aggagaggca ttggaacgag cgggtttttc attagaagat ctaacgcctt
     5401 tgaaagcaat tggtgtaaca ggtttaagaa tggattatca tatttccaat cagcagattg
     5461 ccgactggtc acatcaattg aaaatcagtt tgaatgctag cacgatcacg cctaaagata
     5521 tcgatgaatt gaaagaagca gaagctgatt tctcacagat tgaagcttgg cataattatt
     5581 atccacgtcc cgaaacaggg ctggacaaag aatggtatca gaaaaaaaat caatggctca
     5641 aaattcaagg tctccttgtc caaggttttg taccaggaga tacagagttg agaggcccac
     5701 tatatcaagg attgccaact ttggaagaac atcgagggat ccatcctctt gcaggcgctc
     5761 tagatctatt agcaagtaac acggatattg tttacatcgg agatgcaggt ctgtcagaaa
     5821 acgttcagga gcaatttgct tctttccaaa aagaacagac agtcttgctc catacggaac
     5881 ctgtagatga agaattttac gaatatatat tagggaagca tacgaatcgg caagacgatg
     5941 caagagatgt catacgaagc gtagatgcac gttttcgaga gattccgcct atcccagcaa
     6001 gaaatacagc tacgcgtatg aaagggtcta ttactttaga taacgaaaag tatttgcgct
     6061 atatgggtga gatccaatta accaaatatg atttgccagc ggatgaaaaa gtgaatgtag
     6121 tagctaaagt tatcaaagaa gagctaccat tgatcaacca aatcaaagct ggtatgaact
     6181 atcaatttat aagaaaagaa ggaagataaa aatgagcaaa atcaatttag agaacctaac
     6241 aacggaacgc agaaaccaag aaacttttgg attagatgag atgagtattt ctcaagccgt
     6301 tcaactaatg aacaaagaag atcagaaagt ggccgctgcg gttgaaaaag aactgacaag
     6361 tatcgaacca gtgattgcta acacgattga gtcctttaaa aaaggtggac gtctgattta
     6421 tatgggagcc ggaacaagtg gaagactagg tgtgttagat gctgctgaat gcgtaccgac
     6481 attcggtgta gaaccggaaa tggtcatagg tctaattgct ggtggagaag aagcaatgac
     6541 tgtagctgtc gaaggggccg aagattctta tgagctaggg caagaagatc taatcaagct
     6601 gaatttgaca aaaaatgata tagttattgg tatcgcagct agcggtcgta caccatatgt
     6661 gatcggtgga ttagattacg caaaatcgat cggtgctgtc acaggaacga tttcttgcaa
     6721 taaacaagcc gaaatcagta aacacgcgga tttcccaatc gaagtcgatg caggtccaga
     6781 atttttgaca ggctctactc gattgaagtc aggaacagca caaaaactta tcttgaatat
     6841 gatttctact atcagtatga ttggtatagg taaagtattc aataacttga tggtcgatgt
     6901 caaaccaaca aatgaaaaac taatcgaacg ctcaaaacga atcatcatgc aagcgacaga
     6961 agctgattac gaaacagctg aaaagtactt tgaagaagca gatcaaaatg ttaagctggc
     7021 tatcgtcatg attttgacaa atagtgataa agaggaagct caagcaaaat tagctgaagc
     7081 aaacggattt gtaaaaggga ccttatgagg gggaaaagac catggcaaaa aacgaactga
     7141 ctttggaaga actggcaaaa caaatttata aaggggcagg cggcatgggc aatgtagaaa
     7201 gcattgtcca ctgcatgact cgtgtgcgta tgaagatcat caatgatgat cttgtaaatg
     7261 tctctgaatt aaaagcgatt ccaggagtgc ttggtgtagt agaagatgaa cagcttcaag
     7321 tcatcatcgg acctgggaaa gtgaacaaag tagcacagca aatggttgat ttggcaggcg
     7381 tgaaattagg agaaaaactt tcttctgtaa gtacagctac agcaaattca tcagcatctg
     7441 gaaaagaccg tgtaaacgaa cgggcacaag aaatgaaagc ggcacaaaaa tcgaaacaaa
     7501 aaccatctaa aatcaaatcg gtattaaaag acatttctaa tatctttgtg ccaatgattc
     7561 ctgcatttgt cggtactggg atcgtggcag ggattgccgc agttttagca aacttagtca
     7621 cagccggaac acttgatgca gcaacatggc aacaatatat cgatgtgatg aacatcttga
     7681 aaaacgggat gtttagctac ctagttatct atacaggtat caatgctgca caagtttttg
     7741 gtgcaacacc aactcttggt ggagttatcg gggccgttgt gatgctgaca gggatgaatc
     7801 cggaagcacc actgaaaaac ttatttactg gtgaagctct tgcagctgga caaggtggga
     7861 tcctaggtgt catcttcgct gtttggctat tgtcaatcgt tgagaaaaaa ctgcataaag
     7921 tagtacctga tgcggtagat atcatcgtta caccaactct ttcattgatt gccatcggtc
     7981 tgattgaaat tttcttcatc atgcctttag caggtttcat ttctgatggc atggtcggtg
     8041 ggatcaactg ggtacttggc gttggtggag catttgccgg cttcgttcta ggcacattat
     8101 tcttgccgat ggttatgttc ggactgcatc aaattttgac tccaatccat gtgcaaatga
     8161 ttgatgaaac tggtcgtacg ctattgcttc caattcttgc gatggctggt gcaggacaag
     8221 tgggtgcagc attggcatta tggattcgct gcaaaaaaga taaagaatta acagaaatga
     8281 tcaaaggagc tttaccagtc gggatcctag gaatcggcga accattgatt tacggagtaa
     8341 cattacctct aggacgccca tttattactg cttgtatcgg tggaggtatc ggcggggcag
     8401 tgatcggaat gatcggaaat gtcggggcta tcgctattgg accttcagga gccgctttga
     8461 tcccattgat ctctgacggt aaatggttag gctacatcct tggattattg gcagcttatg
     8521 ctggtggctt cgttgctaca ttcttcttcg gtattcctaa agaccgttta gaaaaagaag
     8581 aattagcgac tgaaacagcc acagtaaatg aagtgcctga aaaagtagta cctcaaactt
     8641 cagctgaaag tgtgactatc ctctcatcag ttgctgacgg aacagttgtt tctttagata
     8701 aagcaagtga tccagttttt gcacaaaaga tgatgggtga aggctacttt gtagaaccag
     8761 aaaacggaca tatctattct ccagttactg gggtagtcag ctctgtcttc ccaacgaaac
     8821 acgctattgg gatcacaacg ccaaatggtt tagaaatctt gcttcatatg ggaatcaaca
     8881 cagttgactt aggcggtacg ccatttgatg tgaaagtaac agaagggcag caagtgacac
     8941 ctgatacgct tgtagcagaa gctgacttag aagcgatcaa agcagcaggc aaagaaacat
     9001 ccatgatcgt cttggtaacc aatatggatc gtgtaaaaaa ctttgtcttg gaaaaaacag
     9061 gtaaagcaaa agcaaaagca cctgtgatgg atgtcaaagc ttaatatgat agaaacaata
     9121 gaaacgaaag aagaaactga aatctatttt tcagcttctt cttttttatt atttattgtt
     9181 ttctgctaaa atctgataaa ttcgtgtcac atcagatggt gttccccgta attcataatc
     9241 tgccaagaaa ggaaagccaa atgtttgtgc atattgtttg gctgttagac aatactgatt
     9301 attgaagttt ttatttccgc taccgactac accaagacaa agtttatgat tgtcttcatg
     9361 ttccagatat tcac
//
LOCUS       DAYYHS010000266         5593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.guided.132, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000266 DAYYHS010000000
VERSION     DAYYHS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.guided.132"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..756
                     /locus_tag="ACHA6A_003128"
     CDS             64..756
                     /locus_tag="ACHA6A_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8971226.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1083..1580
                     /gene="dfrG"
                     /locus_tag="ACHA6A_003129"
     CDS             1083..1580
                     /gene="dfrG"
                     /locus_tag="ACHA6A_003129"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8971227.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1652..3604)
                     /locus_tag="ACHA6A_003130"
     CDS             complement(1652..3604)
                     /locus_tag="ACHA6A_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8971228.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3611..3847)
                     /locus_tag="ACHA6A_003131"
     CDS             complement(3611..3847)
                     /locus_tag="ACHA6A_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971229.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4249..4539
                     /locus_tag="ACHA6A_003132"
     CDS             4249..4539
                     /locus_tag="ACHA6A_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971230.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4811..5160)
                     /locus_tag="ACHA6A_003133"
                     /pseudo
     CDS             complement(4811..5160)
                     /locus_tag="ACHA6A_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5253..5555
                     /locus_tag="ACHA6A_003134"
                     /pseudo
     CDS             5253..5555
                     /locus_tag="ACHA6A_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1895 a          902 c          977 g         1819 t
ORIGIN      
        1 gacattgtgt ttgagatcac ttacttttag catgtaaaag tattggatta aggagctatg
       61 caaatgaaaa agaaatttat tactggtggg attgtcgttg catttctatg tctaattggc
      121 tacacaggca cgcggtacgc aaatgaaatt attttgtggg gtggagagac agatattgat
      181 acaatcaatg agaatctgac acagcttgat catacattga cagataaaga gcaagcgatt
      241 acagatctta ccgctcgact agcacaaaaa gaacagacat tgcaagattc aaaggcagat
      301 gtccaagaat accaaacaaa agtaaatgaa ttagaaaacc aaaaaaatca atcggcagca
      361 gaaaaacaaa acttagaaag tcaattagtc agcaaggatg cacaactaca aagcaaacaa
      421 aatgaaatca atgtcaaact agatgagatc aaccaaaaaa atgatgaaat caatcatctg
      481 aatgcagatt ggacagcaag actacaagaa gcacaagcaa aactttcaga attacaaaat
      541 actgtaaatg gtttgaatca acaaatcgca acactgacac aagaaaaaac aaatgtagaa
      601 actcagttaa cagatacaca aaagaaatta gacgaagcaa ctgctgaaaa ctcaagctgg
      661 aaacaatata ttcaaaagtt agaaaaagca aaagatgaag tggaagatac agctgataaa
      721 tctcagcaaa ttgttgaaga acatttgcgt aaataatctt tcttaacaag taaacctaaa
      781 ttagtaaaga agcaacagta atctatacta agtagttcag ttttggagta caaaatgaca
      841 aaatgtactt caaaatggac tacttttttc attttaactt acataccaaa ttacatacaa
      901 aatgatttat aaaatgcaat tgaaaatgat aattgaatga aaatttgtga ttttgtggta
      961 ttatgataat ataacttata gaaaaaattg cttattcaac taacgggcag gataatgaga
     1021 aaaggttttt tgatagaaat aagaaattag atggtaaatt agttaaaaag gctgtgttat
     1081 aaatgaaagt ttctttgatt gctgcgatgg ataagaatag agtgattggc aaagagaatg
     1141 acattccttg gaggattccc aaggactggg aatatgttaa aaatactaca aagggacatc
     1201 cgataatatt aggtaggaag aaccttgaat caatcggaag agccttacct gacagaagaa
     1261 atattattct gacgagagat aaggggttta cctttaatgg ttgtgaaatt gttcattcaa
     1321 tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat ttttattttc ggaggagaac
     1381 agatttataa tttgtttttc ccttatgttg agaaaatgta catcacaaaa atacatcatg
     1441 aattcgaagg agatactttt tttccagaag tgaattatga ggaatggaat gaggtatttg
     1501 cccaaaaagg gataaagaat gataaaaatc cgtataacta ctattttcat gtatatgaaa
     1561 gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa gaacagtaaa gataaagcac
     1621 aagagcttgg gattactcca agctcttttt gtcatttgaa ataaattata tctttccaat
     1681 caaattgttt atctgtaatc cctaatcttt gagcaggtgt caaaaccttt ttatccgctg
     1741 atttaaaagg gcgacaaaag ttgtagtaag ttcttagaat tgtaatcgca aattgagcat
     1801 acttcggatt gaagttagcg taaatatagc tcttcttatc accacgagct gttgttaatg
     1861 gtctttcaag agatgatata taacgtctta tctgttgaat aaagctattg gtcgaatggt
     1921 cattaacatt taatagcatc tttgcgattt cgtttggttc cagagcagat aaatccgttc
     1981 tacaatcaac tgaataaaat cctttatcct tagtggctaa tggatgtttg atagggttat
     2041 cggcatattt gcgatatctt tctccatctt tactaatagc ctcttcgtgg aattgatgac
     2101 ttgttagtaa ttcagttaga taatgagatg ccaaagtccg tagaaacttt gttttgaatc
     2161 ctctgatatc tccccaatcg agcagttctg cttttgcttg tccgaattct tttaagcatt
     2221 gttttctgct tttcgtcttg ttaacttggc aaataaaatg atgtgcatcg ctcaacctta
     2281 attctttcgt gaatactcta taaaaggcgt ttctaataga gaagtcatca tcactaatca
     2341 ttcgccattc gtcggcatta accatttgtt taattaacca atagtgagca gttgtagtat
     2401 aaggagcatc aacatttaaa ccatcgacaa atctactcct attagtaatt ttccctaatt
     2461 cgtgaagata agcattccta ttttcgctat cgttagcgga tggttcttgt ggataataag
     2521 accaatctaa cctatcattt ttacgactaa aagtattgag atggtcttct ttgaattttc
     2581 ttgtatcttc atttaattca tccattgaaa tattccaatc ataggcaaca tcagaacgga
     2641 acacatatct tgataagacc tcagcagata caactatata cgtttgcata ttaaggtctt
     2701 caaaccccgt atatttcttt gaaccttgtc ctttcttacg gacgttattt aggtagtagt
     2761 gcatcttgtc cgtattaagc cacatctcgt taaatttctt tgtttgaagg ggttgtgtct
     2821 catatcgttc taaaaactcc aaacaacgcc tatacagcca ctccagctta tgatagtagg
     2881 ttccaactcc aattcctaaa atatcacaag tacgactgac tgaaaccctt ccaacaacca
     2941 tcttagcgaa cattggaaga atggtgttct tttgttggtg ataagtagtg gtttcttctc
     3001 tttttggtag aacattagtg aacttcttac acgccttaca ttgatagcgt tgagatttgc
     3061 ccctactttt cccccttttg taaaattgct tcggctcatt gaatggggtt gattcttctt
     3121 ccgagcagcc ttctttatga aattgataat ctggctcaac atcttggatg ctattgattt
     3181 ctataaggcg tttaatttct tcaacaacag accaattaga taaaggggtt actgtacagt
     3241 tttgcgatac acctctgcca ataggattgt cattgcagta cataccttta tgacctttat
     3301 ctttggaact accagtcatt ttatatcgac ttggtttccc ttttacatct ttgaatttgt
     3361 gctgttcttt cccaaaatta caacaaaacg gattgatgca gtaattatat tgaatgggat
     3421 attcagaacc gttccattta aaagacgttg gcaagaacaa cattttgtta taccttgttg
     3481 caaatcgttt tggagttaat aaatcatatt gtttagccct atcttttaat tcttcgtttg
     3541 aaatcggaat tttaacatct acaatttcat cttccttcgt ggctaatctt ttaagcttgg
     3601 tcattgtcta tcaacccact ctatctttga tacccatttt ttcaacaatc cgcttattac
     3661 ttcgcttgtt ggcaatccat tcaataaact tcttatcgtc taaaatgttc aataagaatt
     3721 catccactgc ttcgctttca gtacgctctg catattcagc gtattgctta actatcaccc
     3781 ttacttgctc tgatatttcc caatccacag acttagcgtt cttatttttt ggctcaagaa
     3841 attgcatagg aaaatgcacc cctttttgct tagtaaattg taaccatttt aacacaccaa
     3901 tttgcattcc tacaataatt atttcacgtt cgcattttta tatacatttt aacttacatt
     3961 ttgtggtgta aattaacaac aaacgttgat ttaacaataa aaaacgagta tcaaattcag
     4021 ttgcaatttg atactcattt aggtttacat tttcactttt tgtactccaa aactgaacta
     4081 cttaaatcta tactgctgcc tctttttttt agtacatgtc cttcatcttc gcaggaagtg
     4141 ccaatatgat tttaagaaag gtaatagaca aaaagtcaaa aagataaaac agagtttctt
     4201 ctttacaaag ggaatgtatg ttcgtataat ggtattgagg tgaaggttat ggagaaaaaa
     4261 tatggaacag tagataagat ccgtactttg caagttagtg ccagaccgtt gatccgtttc
     4321 agtttaaatg gggtcaattg tttgattgct tcccatagtt tgaacttcct agctgaagta
     4381 gacgaaggga tgaagttagt cgttacaggt tattataata atagaaagca atttgttgtg
     4441 agatcctatc atgtgttagg gaaacccaaa attgtggtag aatatgagaa aagtctatac
     4501 ccaagaaaaa aggtccgaca agatgaaaga ataaactaga aaacaagcgg ctgagacaaa
     4561 aatgtcctag ccgcttgttt tggaaagatg gatgcagacg atgggatttg aacccacacg
     4621 agcatacgct cacaagaacc tcaatctata tataactaca tgtatttaag tatttgtgat
     4681 gaatcactaa actttccaaa ctcatttgag ggatattgct taaagattga acagatcaat
     4741 tcgaagccct attagtattc gctcaatggg gggatcagag gccataaaaa gagactagtc
     4801 attactagtc tcaggtagtg gaattgtagg attttaaacg ttcctggaga gtaccggtat
     4861 gtaactcgaa gaggtgatta tcatagtcgt aaaagtaaat tgaagaagcc tcgcctttca
     4921 cacgagaacg tcctggttcg acagttaaac ctaacatttg gatttttgag acaaacgaat
     4981 ctatatcttg ttcgtcaatt ttaaaagcaa tatgattgta tgtctttggc agtttattcg
     5041 acgaattctg cattactgca atccataggt ccttaactaa gaaaaatttt tctggatata
     5101 atgaaaattt ttttgttgac tatcgtagat ttcttttgca tcaaataagg tatttaacaa
     5161 ttaagtattt aaaagataac ttttaagtta tctttaagta atgcttaaag ctatgttata
     5221 ctctattagg aaatataaga aggagtataa aaatgaacaa acataataga aaaataacga
     5281 ataacaaaga actgatcttg ttactaatag ggatttgggg tatcttttta ctctttaata
     5341 tgtatatgcc gactatgaga gcggatgacg tagtttatgc aagtagatta gatgaacttg
     5401 gctatttggg tgcttcgata gaacattata agacatggag ttcaagaatt attatagaac
     5461 tattcttaat gttcttttct aaacacttta tgctatggaa attattaaat tctacgatta
     5521 tgttaggaat tgtagtatta ctttgcaagt atgttgagag tataaaatat tttgtgtaaa
     5581 tgaaaaaatc cat
//
LOCUS       DAYYHS010000267         4702 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000267 DAYYHS010000000
VERSION     DAYYHS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.guided.141"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..1000
                     /locus_tag="ACHA6A_003135"
     CDS             194..1000
                     /locus_tag="ACHA6A_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8971231.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1018..1725
                     /locus_tag="ACHA6A_003136"
     CDS             1018..1725
                     /locus_tag="ACHA6A_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8971232.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1722..2693
                     /locus_tag="ACHA6A_003137"
     CDS             1722..2693
                     /locus_tag="ACHA6A_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8971233.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2686..3714
                     /gene="vanB"
                     /locus_tag="ACHA6A_003138"
     CDS             2686..3714
                     /gene="vanB"
                     /locus_tag="ACHA6A_003138"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8971234.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3720..4328
                     /locus_tag="ACHA6A_003139"
     CDS             3720..4328
                     /locus_tag="ACHA6A_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8971235.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4394..4567)
                     /locus_tag="ACHA6A_003140"
     CDS             complement(4394..4567)
                     /locus_tag="ACHA6A_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971236.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1329 a          986 c         1245 g         1142 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata
       61 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
      121 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
      181 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
      241 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
      301 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
      361 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
      421 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
      481 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
      541 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
      601 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
      661 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
      721 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
      781 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
      841 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
      901 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
      961 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     1021 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     1081 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     1141 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     1201 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     1261 ttaaacggct tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg
     1321 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     1381 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     1441 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     1501 caggtgttcg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     1561 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     1621 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     1681 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     1741 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     1801 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     1861 ctggcaatca atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg
     1921 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     1981 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     2041 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     2101 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     2161 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     2221 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     2281 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     2341 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     2401 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     2461 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     2521 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     2581 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     2641 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     2701 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata
     2761 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     2821 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     2881 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     2941 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     3001 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     3061 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     3121 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     3181 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
     3241 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
     3301 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
     3361 attgtcggcg aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa
     3421 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
     3481 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
     3541 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
     3601 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
     3661 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
     3721 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
     3781 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
     3841 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
     3901 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
     3961 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
     4021 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
     4081 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
     4141 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
     4201 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
     4261 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
     4321 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
     4381 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
     4441 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
     4501 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
     4561 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
     4621 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa
     4681 gttgaactat ttaatggact gt
//
LOCUS       DAYYHS010000268         3932 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.guided.158, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000268 DAYYHS010000000
VERSION     DAYYHS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.guided.158"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(202..572)
                     /locus_tag="ACHA6A_003141"
                     /pseudo
     CDS             complement(202..572)
                     /locus_tag="ACHA6A_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            717..902
                     /locus_tag="ACHA6A_003142"
     CDS             717..902
                     /locus_tag="ACHA6A_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8971237.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1060..1227
                     /locus_tag="ACHA6A_003143"
     CDS             1060..1227
                     /locus_tag="ACHA6A_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8971238.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1845..2504
                     /gene="vanR-B"
                     /locus_tag="ACHA6A_003144"
     CDS             1845..2504
                     /gene="vanR-B"
                     /locus_tag="ACHA6A_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8971239.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2507..3868
                     /gene="vanS-B"
                     /locus_tag="ACHA6A_003145"
     CDS             2507..3868
                     /gene="vanS-B"
                     /locus_tag="ACHA6A_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8971240.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         1129 a          811 c          978 g         1014 t
ORIGIN      
        1 cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt
       61 gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat
      121 agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa
      181 aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat
      241 tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt
      301 cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa
      361 gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg
      421 aatggcccgt cgttttcgat gttggcaaga tagcgccagt caatatttat tttctctgcc
      481 attgctttgc gcgaaacgcg ctttgcttgc cgggccgctt tcacatccgc gccgaaagtt
      541 tcaaagccgg gacaatcttc aatattcgcc atatcgcacc tcccacttac attgtatatt
      601 tcatactctg cccgtggaat gttggtatat gcacctttct taaagtttat atttcacatt
      661 ttgtttcttt tattcttcga gcaaaagaat tataatgtac cctgtggagg tgtactatgg
      721 actacatgac gttgaaagag gcaggcgaga aatggggtgt gcccccacga tggataaact
      781 actattgtgc tgcgggacgc atccccggcg ctgtaaagat ggcgacgatc tggctcattc
      841 ccaaaactgc ggaaaagccg attgacaggc gtacaaagaa aggaaaggcg gttactgaat
      901 agtataatga aaccggcgca ttttggatat ggttttgaag agagagaaag catggtattt
      961 tgtcacggaa aaactataag atgatagatg gcaggctgct ccagaccgat aaaaagtatt
     1021 ctcatctgaa acagaaagaa aagatttaca catggatgta tgaagaagcg aaactttatc
     1081 atgacagaaa tggaaaatgc ccgaaacaaa gacgaggaag ctgctgctgt atcagccgta
     1141 tatggtagaa ttgaaaaggc cggcatctgg attctttatg gtgaagtctc cagacattgg
     1201 ataactgctg gtctaatgcg cctttgagta tggggtgatt gattttaata ccggggaggt
     1261 acggcttttt gaggaaaata ttctgggatt tacatttggc gattattaca tttcctgtaa
     1321 ctgcaaggac acggatgatg aagatatgag gaatatagac ttgctgattt tctactgtct
     1381 tgaacggtat taggactgaa tctgctgcgc ggagtgcggc aagagcgacc ggagggagta
     1441 cacacaggcc ctgcctgtgt gaaaagccat accgcaaggt gcattcgcaa aaaagaggcc
     1501 gcgcattttg ctgaggcttg gggtgtcccc aacaagcaaa acgagaacgc cgcaggcggg
     1561 caagtggtgg atcagaatat ccgtaaagga tattctgatc cattgcttgc cagtattatc
     1621 gggatgtttt gacaaccgct gtaatcgaaa catcccaaac attaagaagg tgttaattat
     1681 tattatcttg atttaattcg tttaaaccaa taatatagat acatacacca tgaaaagaag
     1741 cgagtatccc aatgacggat gattaaaatc cgaaaagaaa tcgctttgaa actacaggga
     1801 aactacagac tgttatgtta tcttcttaaa atggagggat ttttatgtcg atacgaattc
     1861 tacttgtcga ggatgatgat catatctgta atacagtaag ggcgtttctg gctgaggcag
     1921 gatatcaggt ggatgcctgc acagatggaa atgaggcata caccaagttt tacgaaaaca
     1981 cttatcaact ggttattctt gatattatgc tgcccggtat gaacgggcat gaacttttgc
     2041 gtgaatttcg tgcgaaaaat gatactccca ttctgatgat gacagccctg tcggatgacg
     2101 aaaaccaaat ccgggcgttt gatgcagagg cagacgacta tgtaacaaag ccattcaaga
     2161 tgcagatttt actaaagcgg gtggaagccc tgctgcggcg cagcggtgcg ctggcaaagg
     2221 aaatccgtgt cggcaggctg acacttctgc cggaggattt cacggtactt tgtgacggta
     2281 cagagctgcc cctgacacga aaagaatttg aaatcctttt gctgctggtg cagaacaaag
     2341 gcagaacctt aacgcatgaa atcattttgt cacgtatatg gggatatgac tttgagggtg
     2401 atggcagcac agtccacact catatcaaaa atttgcgggc caagctgccg gaaaatatca
     2461 tcaaaaccat tcgcggtgta ggataccgat tggaggaatc ataataatgg aaagaaaagg
     2521 gattttcatt aaggtttttt cctatacgat cattgtcctg ctactgcttg tcggtgtaac
     2581 agcaacactg tttgcacagc aatttgtgtc ttatttcaga gtgatggaat tacagcaaac
     2641 agtaaaatcc tatcagccat tggtggaact gattcagaat agcgataggc ttgatattca
     2701 agaggtggca gggctgtttc actacaataa ccaatccttt gagttttata ttgaagataa
     2761 agagggaagc gtactctatg ccacaccaaa tgccaataca tcaaatagtt ttagacccaa
     2821 ctttctttat gtggtacata gagatgataa tatttcgatt gttgctcaaa gcaaggcagg
     2881 tgtgggattg ctttatcaag ggctgacaat tcggggaatt gttatgattg cgataatggt
     2941 tgtattcagc cttttatgcg cgtatatctt tgcgcggcaa atgacaacgc cgatcaaagc
     3001 cttagcggac agtgcgaata aaatggcaaa cctgaaagat gtaccgccgc cgctggagcg
     3061 aaaggatgag cttggcgcac tggctcatga tatgcattcc atgtatgtca ggctgaaaga
     3121 aaccatcgca aggctggagg atgaaatcgc aagggaacat gagttggagg aaacacagcg
     3181 atatttcttt gcggcagctt ctcatgagct aaaaacgccc atcgcggcta caagcgttct
     3241 gttggaggga atgcttgaaa atatcggtga ctacaaagat cattctaagt atctgcgcga
     3301 atgcatcaaa atgatggata ggcagggcaa aatcatttcc gaaatactgg agcttgtcag
     3361 cctgaatgat gggagaatcg tacccatagc tgaaccgttg gacatagggc gcacggttgc
     3421 cgagttgctg cccgattttc aaaccttggc agaggcaaac aaccagcggt tcgtcacaga
     3481 tattccagcc gggcaaattg tcctgtccga tccgaggctg ctccaaaagg cactatccaa
     3541 tgtcatattg aatgcagttc agaacacgcc gcagggaggc gaggtacgga tatggagtga
     3601 gcctggtgct gaaaaatgcc gcctttttgt tttgaacatg ggcgttcaca ttgatgatac
     3661 tgcgcttcca aggctgttca ccccattcta tcgcattgat caggcgcgaa gcagaaaaag
     3721 tgggcgaagc agaaaaagtg ggcgaagcgg tttaggactt gccatcgtac aaaaaacgct
     3781 ggatgccatg agcctccagt atgcgctgga aaacacctcg gatggcgttt tgttctggct
     3841 ggatttaccg ctcacatcaa cattgtaaat atttaaaact taaatgattt tgggctcttt
     3901 gtcaaatagg actgatagag tgagataaga ac
//
LOCUS       DAYYHS010000269         3816 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.guided.164, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000269 DAYYHS010000000
VERSION     DAYYHS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.guided.164"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..674
                     /locus_tag="ACHA6A_003146"
     CDS             39..674
                     /locus_tag="ACHA6A_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8971241.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            810..902
                     /locus_tag="ACHA6A_003147"
                     /pseudo
     CDS             810..902
                     /locus_tag="ACHA6A_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            1016..1651
                     /locus_tag="ACHA6A_003148"
     CDS             1016..1651
                     /locus_tag="ACHA6A_003148"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8971242.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            1758..2996
                     /gene="mef(H)"
                     /locus_tag="ACHA6A_003149"
                     /gene_synonym="mefH"
     CDS             1758..2996
                     /gene="mef(H)"
                     /locus_tag="ACHA6A_003149"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8971243.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            3223..3642
                     /locus_tag="ACHA6A_003150"
     CDS             3223..3642
                     /locus_tag="ACHA6A_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8971244.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            3647..>3816
                     /locus_tag="ACHA6A_003151"
     CDS             3647..>3816
                     /locus_tag="ACHA6A_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8971245.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVD"
BASE COUNT         1305 a          534 c          735 g         1242 t
ORIGIN      
        1 agcatgatag acttataata gcgaagatat aacgagatat ggagggttat gcaatgaaaa
       61 taagccattt aggaaataat atacagacca taagaaaatt taggggaatg aaacaacagg
      121 aacttgcgga caaaatcggt atcaatatgc agagcctttc caagattgaa agagggttga
      181 actatcctgc ctatgaaacg ctggaaaaga taatggaagt gctggacgtt acgcccaatg
      241 aattgttgtc gggagaatgg aagtatgtca atcaatctga aaaggaagtc tgtcagtttt
      301 taagagccga agaacgtcta aatgcagaac tgaaacatgg acactatgat aacttctttg
      361 acagcgagga agaatggctg gaatatgagt tagaaaagtt acgggaatat attacagact
      421 atatcaacgg gaaaagcatt gaagcgtccg acctttaccc aatcaaagaa tttatccagc
      481 atttgaagtt tcaaaaatta ttagaccgct atgatgattt atacagtatg gatatgtttg
      541 gggaaagcat agagggacat aagtataaga caccttatca agtcgtgaaa atgataaacc
      601 cgaactcaaa agaagatatg gaactgttac gagaagtatt aaggaataac aattttgatg
      661 atgaggacga gtaacttttt tcctcttgcg agaagataca gcaaacaaaa agcccgatca
      721 agagtgcaaa gcaccaccaa tggtggcaaa gctcttgaca gggctttttc ttttctgttg
      781 tgggcaatca agaggaagaa agggataaaa tctgatttgt ggtaaatcag gcaaaggcaa
      841 cgcaggtatc acagtatgaa ttgacaccgc ataaggaaag caactggaag attgtagaat
      901 aaatgaaaaa ggcttgcatt ctgcattttt ataaaattgc aagtctttat attgacaagc
      961 ataaaatact aagatatact ttaatcagac caacggtttg tattggaggt aagttatggc
     1021 acgaaataaa tatccagaag aaacagtaaa actcatttta gatgtttcta caaaaatgtt
     1081 ttgtgaaaaa ggatatgata acacatcatt gcaagatatt atcaatgaaa caaaactttc
     1141 taaaggtgct atttatcatc atttcgattc aaaggaagat attttaaaag caatttttca
     1201 aagaattgga aatgaaaatg cagtaatatt tgctaaaatt agagataacg atagtttaaa
     1261 cggtttagaa aagttaagag aaatatttaa gacagcggta ttcaattcta accaaagcgt
     1321 tttattgaca gtttctcctc aactattaaa caatcctcgt tttcttgcta tgcaaataga
     1381 acaaatgtat gaaattgtag tacctgaatt tgttgaaccc gttttagaac aaggtatcaa
     1441 ggataagtct ttggagattg aaaattcaca tgaaattgca gaagttatca tggtattaag
     1501 taatgtatgg ctcaacccat tagttgaaat gacggataaa gagggtatgg aaaaacgttg
     1561 caatacattc aatgcccttt taaatggaat aggcatagac acattattag acgaagaaac
     1621 aatttctgca tttatacagt tttgtaaata actgtatttg ttatccctat acgatagact
     1681 gctctataaa agagcagttt atttaaacca ttatataaac cgacggtctg tttgattaaa
     1741 taaaaaggag agattttatg caagcagaaa aaaaactttt taatcgtgat tttatacttg
     1801 ttgttatagg acagataatt tctttatttg gaaacgctat cctacgattt gcattaccgt
     1861 actatttgtt aaaagaaact ggttcatcag ctttgtttgg tattgctaca gcctgctctt
     1921 ttttacctat ggttattttg tcgctggtag gtggtattat tgctgacagg acaaataaaa
     1981 gaaacattat ggtcatacta gattttgtaa cagcaatcat aattttagta ttttatttgt
     2041 cattgggaaa aattcctatc atacctttgt ttattgttgc tttgatgtta ctttatggta
     2101 tttctggtgc atatcaacca gcggtacaag caagtattcc tctattggta tcatcagata
     2161 aacttaccgt cggtaatgct ataataaatc aagtaaatac tttagcaaac tttatcggac
     2221 cagctataag cggagtagtt tttcaatttt atggtatcaa tcccatttta attgttagta
     2281 ttatatgttt tactttttca gccattatgg aaatttttat ccatattcca caccaaaaaa
     2341 gacctcgtga agtaggtgta attgcagttg ctaaaaacga tttaaaagat agttatacct
     2401 ttttgaaaaa ggagaaaccc atttttatat ccgtaatctt tttggtttgc attttcaatt
     2461 tggttttaag tgctgtttta attgttggtc tgcctatctt aatttctgaa gttttaaaaa
     2521 tgccagatac actatatggt ttttcacaat cagcattatc attaggtggt ttatgtggtg
     2581 gtattttaac agctcttgtt gcttcaaagt taaaactaaa aaacctttat accttgttac
     2641 ttatttgttc tgtttctgtt ttacttatgg gaatttcgct atttttagaa ttgcctgcaa
     2701 tagtttccta ctgggtagtt acagtgatgt gctttgtggc tatgggtgta tctactatgt
     2761 ttgttgttca aatttacact cttgttcaga cacaaacacc accgcaatta gtaggaaaga
     2821 taatggctac gctggtagca gttgctatgt gcggtcaacc tattggacaa atggtttacg
     2881 gtattttatt cgatgtattt atacaagaaa catgggttgt tatgtttgta gcttctattg
     2941 cttcaatcat gatttcaata tattcaaaaa gaatttttca aaagttagag atataataca
     3001 aggggttaaa tagtatgtta ggctaaaaac aatatataaa agaaaatttg aaatctgcta
     3061 aaaggatagt gttataaaag aaagagtaat attgcagtat ttaaggctcg cacaaatata
     3121 tgttttgtgt gggctttttt cttaccattt ttctgtattt ctaaagggta taaacggtat
     3181 tccgtttata tatttcagca ttagcagaaa gggggtgaga ttatgaggac atcttctttc
     3241 gagcatatcg tcagattaca gttcaattct ttaatgctga ttgtcattaa gaacaaagtg
     3301 aaaagtcgaa acagacagct tgcaagacat tctaaacgtg aagtattgtt ttgtgaaata
     3361 gccgaaacga aacaagttga acgtggaacg attgatacct gtttttatga ttgtgtatca
     3421 tttaaagtct tgcactttac aatttatgta tctgatgaaa cacttggtac agttttacat
     3481 agactgtcag aaaaacagcg tagtgcgatt ttattacgtt attttcaagg aatgaacgac
     3541 agagaaattt cagaactgta ccatgtatca cgttctgcta tttgcagtag aagaactaga
     3601 ggactaagga agttaaaaat gcttttgagc gaaaggaagt aaagaaatga gtaaagatta
     3661 tggctatccc tcttttgaat tgatttgcaa agcgtcaagc ggagatgaaa tggcagttaa
     3721 agagattttg aagttttatg acggttatat ttctaaatta tgcttgcgtc ctttttacca
     3781 ctctgaaagt ggtaaaatca ttatgcaggt tgatga
//
LOCUS       DAYYHS010000270         3796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000270 DAYYHS010000000
VERSION     DAYYHS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.guided.165"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHA6A_003152"
     CDS             110..193
                     /locus_tag="ACHA6A_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8971246.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHA6A_003153"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHA6A_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8971247.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHA6A_003154"
     CDS             complement(1318..2181)
                     /locus_tag="ACHA6A_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8971248.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHA6A_003155"
     CDS             complement(2183..2455)
                     /locus_tag="ACHA6A_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8971249.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHA6A_003156"
     CDS             complement(2472..2681)
                     /locus_tag="ACHA6A_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8971250.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACHA6A_003157"
     CDS             complement(2759..3655)
                     /locus_tag="ACHA6A_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8971251.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
LOCUS       DAYYHS010000271          867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7939
            SAMEA115709381-rid22597103.guided.234, whole genome shotgun
            sequence.
ACCESSION   DAYYHS010000271 DAYYHS010000000
VERSION     DAYYHS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709381
            Sequence Read Archive: ERR13199099
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290443.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290443.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:28:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,158
            CDSs (total)                      :: 3,095
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 34 of 87
            Pseudo Genes (incomplete)         :: 40 of 87
            Pseudo Genes (internal stop)      :: 29 of 87
            Pseudo Genes (multiple problems)  :: 13 of 87
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709381-rid22597103.guided.234"
                     /isolate="VRE7939"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..802
                     /gene="aac(6')"
                     /locus_tag="ACHA6A_003158"
     CDS             254..802
                     /gene="aac(6')"
                     /locus_tag="ACHA6A_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8971252.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          310 a          127 c          182 g          248 t
ORIGIN      
        1 attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt
       61 tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat
      121 aattttttat tttataagta agataaataa tgaaagaata gagatactga ttggtagcta
      181 aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca
      241 agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca
      301 gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga
      361 agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt
      421 agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt
      481 agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca attacttaga
      541 aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca
      601 tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat
      661 ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg
      721 tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat
      781 tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa
      841 ataagaagga atttacgaaa attactt
//
